CN101173221A - Wheat cultivation Cordyceps militaris method - Google Patents

Wheat cultivation Cordyceps militaris method Download PDF

Info

Publication number
CN101173221A
CN101173221A CNA2007101662786A CN200710166278A CN101173221A CN 101173221 A CN101173221 A CN 101173221A CN A2007101662786 A CNA2007101662786 A CN A2007101662786A CN 200710166278 A CN200710166278 A CN 200710166278A CN 101173221 A CN101173221 A CN 101173221A
Authority
CN
China
Prior art keywords
grams
cultivation
gram
bottle
cordyceps militaris
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CNA2007101662786A
Other languages
Chinese (zh)
Other versions
CN100575475C (en
Inventor
李景香
赵秀来
孙爱民
乔善成
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN200710166278A priority Critical patent/CN100575475C/en
Publication of CN101173221A publication Critical patent/CN101173221A/en
Application granted granted Critical
Publication of CN100575475C publication Critical patent/CN100575475C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method of cordyceps militaris cultivated with wheat with desquamatory wheat berry, silkworm egg powder and nutrient solution as the canned medium. The invention has the advantages: the invention adopts wheat as the medium of cultivating the cordyceps militaris, which not only saves large production cost of above 30% based on calculation, but also raises the yield and quality of the cordyceps militaris; the invention realizes high quality, high yield, low consumption and high efficiency, and lays a good foundation for popularizing the technology in large scale; the invention is pollution free and a green product which is good for the nation and the people, benefits mankind, has medicine and food function, and has remarkable economic and social benefits.

Description

The method of wheat cultivation Cordyceps militaris
Technical field the invention belongs to the fungus growing technique field, is specifically related to a kind of method of wheat cultivation Cordyceps militaris.
The background technology Cordyceps militaris is the abbreviation of Cordyceps militaris (L.) Link., and the formal name used at school Cordyceps militaris (L.) Link. belongs to Mycophyta, Ascomycetes, Hypocreales, Clavicipitaceae, Cordyceps.It is a kind of very rare medicine-food two-purpose fungi.Its main chemical ingredients, pharmacological effect are very similar to wild cordyceps, detection through authoritative departments such as Chinese Academy of Medical Sciences drug research analyzer room, Shenyang Branch of Chinese Academy of Sciences physical and chemical testing centers shows that main nutrition of some of Cordyceps militaris and pharmaceutical compositions such as cordycepin, Cordyceps polysaccharide, protein etc. are higher than Cordyceps sinensis.Tame northern Cordyceps militaris entity dry product color is orange to fragrance tangerine, that have uniqueness; Delicious flavour, contain rich in protein and amino acid.Uniqueness contains: cordycepin, cordycepic acid, Cordyceps polysaccharide, SOD enzyme isoreactivity material, the man-machine internal milieu of energy comprehensively regulating, antiviral, strengthen the function of scavenger cell in the body, suppress tumor growth, the prevention cardio-cerebrovascular disorder strengthens and the comprehensive adjustment immune function of human body, delay senility, raising human body resistance against diseases etc. has remarkable efficacy.At present, both at home and abroad the research of Cordyceps militaris is mainly concentrated on the initial analysis of cultivating method and technology, effective constituent and to the preliminary study of Cordyceps militaris pharmacological action.Modern study shows and contain ucleosides, polyose, cordycepic acid (D-N.F,USP MANNITOL), sterols in the Cordyceps militaris to also have main components such as amino acid and trace element in addition, and is basic identical with Cordyceps sinensis.Cordycepin is also arranged in the bibliographical information Cordyceps militaris than Cordyceps sinensis height, and the content of Cordyceps polysaccharide and adenosine is lower than Cordyceps sinensis.Amino acid and trace element are approaching with Cordyceps sinensis in the Cordyceps militaris in addition, and Cordyceps militaris protein is made of 18 seed amino acids after testing, contains 8 seed amino acids of needed by human, A wide selection of colours and designs and abundant amount, and ratio is suitable, accounts for 35.47% of total amino acid content.Amino acid is the fundamental unit of constitutive protein matter molecule, and protein is the basic substance of life, is the main moiety of cell, is one of indispensable material of body.Amino acid can participate in synthetic various enzymes, hormone, nucleic acid, protein etc., except that the growth of keeping tissue, renewal, repairing and supplying energy, also has physiologically active widely, regulates the metabolism of body, participates in the synthetic regulation and control of life genetic material etc.Pharmacological research finds that Cordyceps militaris has result of treatment to diseases such as immunity system, neural system and cardiovascular systemss, and has antitumor and antifatigue, calmness and hormone-like effect.Very similar to the pharmacological action of Cordyceps sinensis.In sum, Cordyceps militaris has good exploitation prospect and economic benefit.The cultivation of prior art Cordyceps militaris is culture medium raw material with the rice mainly, adds a small amount of auxiliary material of people plastic bag cultivation Cordyceps militaris again.Because rice cost height originates from south, so be not suitable for promoting in the northern area big area.
Summary of the invention the objective of the invention is to solve prior art cultivation cost height, is not suitable for the technical problem in the popularization of northern area big area, provides a kind of method of wheat cultivation Cordyceps militaris, to overcome the deficiencies in the prior art.
The method of wheat cultivation Cordyceps militaris of the present invention has been taked following technical proposals: operate by following step:
1, spawn culture: bottle is planted the Cordyceps militaris entity behind the separate tissue purifying, through the solid test tube 18~22 ℃ down cultivate 6~7 husbands after, insert the liquid Cans again and leaving standstill cultivation under 18~22 ℃ the temperature after 24 hours, every day early, evening, each manually shook bottle 0.5 minute, see that through 6~7 days cultivation a large amount of mycelia sheets are arranged in the substratum, promptly can be used for cultivation;
Solid test tube substratum wherein: potato 200 grams, glucose 20 grams, silkworm egg powder 20 grams, sal epsom 1 gram, ammonium citrate 1 gram, potassium primary phosphate 3 grams, vitamins B 11000 milliliters in 1, agar 20 grams, water, sterilization routinely;
Liquid can flask culture base wherein: 1000 milliliters of glucose 20 grams, peptone 20 grams, sal epsom 20 grams, citric acid tri-amonia 1 gram, potassium primary phosphate 3 grams, water, under 147 kPas of air pressure, sterilized 40 minutes;
2, Cans cultivation:
(1) preparation nutritive medium: peptone 7 grams, glucose 12 grams, potassium primary phosphate 1 gram, sal epsom 1 gram, citric acid ammonia 1 gram, vitamins B 11, water mix for 1000 milliliters;
(2) bottling sterilization: with 60 milliliters of wheat 35 grams, silkworm egg powder 1 gram, the nutritive mediums of decortication, in the 500 milliliters of Cans of packing into. seal with polypropylene film, 100 ℃ of normal-pressure sterilizations 8 hours;
(3) cooling inoculation: when temperature in the bottle is cooled to 25 ℃, can inoculate;
(4) cultivation management: enter the aseptic culture chamber after the inoculation and cultivate, the mycelial growth stage in early stage: require temperature to be controlled at 15~20 ℃, atmospheric moisture should keep about 65%; Mid-term the sporophore cultivation stage: when mycelia is covered with charge level, put bottle and see light, and on the film of each bottle, prick three to four eyes ventilations with toothpick, after charge level is seen the light annesl, scrape mycelium on the charge level with bamboo chip, scrape and spill till the wheat, scrape 5~6 days careless teeth in back and emerge, require temperature at 20~25 ℃, whole day illumination in 24 hours treats will keep after careless tooth is appeared the light application time of 10 hours every days, and atmospheric moisture is 85~90%, window every day and ventilated 30 minutes, cultivated altogether 60 days;
3, to gather:, when the Chinese caterpillar fungus surface has yellow powder shape thing to occur, can gather when the natural plant height degree reaches about 8 centimetres. when gathering, remove sealing film, with sharp knife substratum is cut and do two halves, take out in bottle, under hand getting, classification oven dry then is with the packed sealing of film.
The present invention adopts wheat to cultivate Cordyceps militaris as substratum, has not only saved the mass production cost, has also improved Cordyceps militaris output and quality.Realized high-quality, high yield, low consumption, efficient, promoted this technology for big area and have laid a good foundation.
1, be example with 50 square metres of cultivated areas, can place 260 bottles for every square metre, dry product 1040 grams of gathering, every gram is worth 0.5 yuan, takes in 520 yuan, can realize 26000 yuan of the gross output values, and behind the deduction cost, 50 square metres of cultivated area net return can reach 23000 yuan;
2, adopt the inventive method cultivation, invest 52 yuan for every square metre, save 16.4 yuan of investments for every square metre, reduce cost more than 30% than the prior art cultivation; As carry out big area and promote, economic benefit is very considerable.
3, this project does not have " three wastes " and produces, be one benefit the nation, green product favorable to the people, that bring benefit to the mankind, medicine-food two-purpose, economical, societal benefits are very remarkable.
One cultivation family introduction rice bottle carries the Cordyceps militaris entity to embodiment from Jiangsu, inserts Cans and make the grass experiment after separate tissue purifying and solid test tube enlarged culturing. and it is good to select grass, does commerial growing, and concrete operations are as follows:
1, solid test tube substratum: potato 200 grams, glucose 20 grams, silkworm egg powder 20 grams, sal epsom 1 gram, ammonium citrate 1 gram, potassium primary phosphate 3 grams, vitamins B 11000 milliliters in 1, agar 20 grams, water, sterilization routinely;
2, liquid can flask culture base: 1000 milliliters of glucose 20 grams, peptone 20 grams, sal epsom 20 grams, citric acid tri-amonia 1 gram, potassium primary phosphate 3 grams, water, under 147 kPas of air pressure, sterilized 40 minutes;
3, spawn culture: the bacterial strain of separation and purification, descend cultivation after 6~7 days through the solid test tube at 20 ℃, insert the liquid Cans again and leaving standstill cultivation under 20 ℃ the temperature after 24 hours, every day early, evening, each manually shook bottle 0.5 minute, cultivation through 6~7 days, seeing has a large amount of mycelia sheets in the substratum, promptly can be used for cultivation;
4, Cans cultivation:
(1) preparation nutritive medium: peptone 7 grams, glucose 12 grams, potassium primary phosphate 1 gram, sal epsom 1 gram, citric acid ammonia 1 gram, vitamins B 11, water mix for 1000 milliliters;
(2) bottling sterilization: with 60 milliliters of wheat 35 grams, silkworm egg powder 1 gram, the nutritive mediums of decortication, in the 500 milliliters of Cans of packing into. seal with polypropylene film, 100 ℃ of normal-pressure sterilizations 8 hours;
(3) cooling inoculation: when temperature in the bottle is cooled to 25 ℃, can inoculate; Inoculation can be taked dual mode: solid test tube inoculation method: will insert in the Cans under aseptic condition through moving the cordyceps species that the test tube that connects cultivates for twice, every test tube can connect 25~30 bottles, for making media surface allow the mycelia front cover reduce living contaminants as early as possible, postvaccinal bottle will shake bottle one by one, and method is; Hand takes Cans to rock back and forth, allows the bacterial classification piece rollback on charge level that inserts move, and rolls tight; The liquid-spawn inoculation method; With cultured Cans liquid spawn, under aseptic condition, pour in the little atomizer, in transfer room, take the can bottle on the other hand and raise one jiao gently, take little atomizer to spray into rapidly in the bottle on the other hand, even spray and seal bottleneck rapidly twice;
(4) cultivation management: enter the aseptic culture chamber after the inoculation and cultivate, the mycelial growth stage in early stage: require temperature to be controlled at 15~20 ℃, atmospheric moisture should keep about 65%; Mid-term the sporophore cultivation stage: when mycelia is covered with charge level, put bottle and see light, and on the film of each bottle, prick three to four eyes ventilations with toothpick, after charge level is seen the light annesl, scrape mycelium on the charge level with bamboo chip, scrape and spill till the wheat, scrape 5~6 days careless teeth in back and emerge, require temperature at 20~25 ℃, whole day illumination in 24 hours treats will keep after careless tooth is appeared the light application time of 10 hours every days, and atmospheric moisture is 85~90%, window every day and ventilated 30 minutes, cultivated altogether 60 days;
3, to gather:, when the Chinese caterpillar fungus surface has yellow powder shape thing to occur, can gather when the natural plant height degree reaches about 8 centimetres. when gathering, remove sealing film, with sharp knife substratum is cut and do two halves, take out in bottle, under hand getting, classification oven dry then is with the packed sealing of film.

Claims (1)

1. the method for a wheat cultivation Cordyceps militaris is characterized in that by following step operation:
1), spawn culture: bottle is planted the Cordyceps militaris entity behind the separate tissue purifying, descend cultivation after 6~7 days through the solid test tube at 18~22 ℃, insert the liquid Cans again and leaving standstill cultivation under 18~22 ℃ the temperature after 24 hours, every day early, evening, each manually shook bottle 0.5 minute, see that through 6~7 days cultivation a large amount of mycelia sheets are arranged in the substratum, promptly can be used for cultivation;
Solid test tube substratum wherein: potato 200 grams, glucose 20 grams, silkworm egg powder 20 grams, sal epsom 1 gram, ammonium citrate 1 gram, potassium primary phosphate 3 grams, vitamins B 11000 milliliters in 1, agar 20 grams, water, sterilization routinely;
Liquid can flask culture base wherein: 1000 milliliters of glucose 20 grams, peptone 20 grams, sal epsom 20 grams, citric acid tri-amonia 1 gram, potassium primary phosphate 3 grams, water, under 147 kPas of air pressure, sterilized 40 minutes;
2), Cans cultivation:
(1) preparation nutritive medium: peptone 7 grams, glucose 12 grams, potassium primary phosphate 1 gram, sal epsom 1 gram, citric acid ammonia 1 gram, vitamins B 11, water mix for 1000 milliliters;
(2) bottling sterilization: with 60 milliliters of wheat 35 grams, silkworm egg powder 1 gram, the nutritive mediums of decortication, in the 500 milliliters of Cans of packing into. seal with polypropylene film, 100 ℃ of normal-pressure sterilizations 8 hours;
(3) cooling inoculation: when temperature in the bottle is cooled to 25 ℃, can inoculate;
(4) cultivation management: enter the aseptic culture chamber after the inoculation and cultivate, the mycelial growth stage in early stage: require temperature to be controlled at 15~20 ℃, atmospheric moisture should keep about 65%; Mid-term the sporophore cultivation stage: when mycelia is covered with charge level, put bottle and see light, and on the film of each bottle, prick three to four eyes ventilations with toothpick, after charge level is seen the light annesl, scrape mycelium on the charge level with bamboo chip, scrape and spill till the wheat, scrape 5~6 days careless teeth in back and emerge, require temperature at 20~25 ℃, whole day illumination in 24 hours treats will keep after careless tooth is appeared the light application time of 10 hours every days, and atmospheric moisture is 85~90%, window every day and ventilated 30 minutes, cultivated altogether 60 days;
3), gather:, when the Chinese caterpillar fungus surface has yellow powder shape thing to occur, can gather when the natural plant height degree reaches about 8 centimetres. when gathering, remove sealing film, with sharp knife substratum is cut and do two halves, in bottle, take out, under hand getting, classification oven dry then is with the packed sealing of film.
CN200710166278A 2007-11-09 2007-11-09 The method of wheat cultivation Cordyceps militaris Expired - Fee Related CN100575475C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN200710166278A CN100575475C (en) 2007-11-09 2007-11-09 The method of wheat cultivation Cordyceps militaris

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN200710166278A CN100575475C (en) 2007-11-09 2007-11-09 The method of wheat cultivation Cordyceps militaris

Publications (2)

Publication Number Publication Date
CN101173221A true CN101173221A (en) 2008-05-07
CN100575475C CN100575475C (en) 2009-12-30

Family

ID=39421979

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200710166278A Expired - Fee Related CN100575475C (en) 2007-11-09 2007-11-09 The method of wheat cultivation Cordyceps militaris

Country Status (1)

Country Link
CN (1) CN100575475C (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940131A (en) * 2010-10-09 2011-01-12 镇江市食用菌研究所 Method for culturing cordyceps militaris by using drink bottle
CN102823806A (en) * 2012-09-20 2012-12-19 王永显 Method for producing fermented oatmeal using Cordyceps militaris
CN105766205A (en) * 2016-04-01 2016-07-20 湖北未来家园高科技农业有限公司 Harvesting method of Cordyceps taishanensis
CN107125025A (en) * 2017-05-23 2017-09-05 高必红 A kind of cordyceps militaris cultivation method
CN109355202A (en) * 2018-11-22 2019-02-19 陈克飚 A kind of Cordceps militaris cultural method
CN112831383A (en) * 2019-11-25 2021-05-25 湖南金芙农业科技有限公司 Cordyceps militaris culture medium wine and preparation method thereof
CN115568380A (en) * 2022-11-08 2023-01-06 天水众安生物科技有限责任公司 Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof
CN115777444A (en) * 2022-12-07 2023-03-14 天水众安生物科技有限责任公司 Method for cultivating cordyceps militaris by using wheat kernels as culture medium

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940131A (en) * 2010-10-09 2011-01-12 镇江市食用菌研究所 Method for culturing cordyceps militaris by using drink bottle
CN102823806A (en) * 2012-09-20 2012-12-19 王永显 Method for producing fermented oatmeal using Cordyceps militaris
CN102823806B (en) * 2012-09-20 2013-09-25 王永显 Method for producing fermented oatmeal using Cordyceps militaris
CN105766205A (en) * 2016-04-01 2016-07-20 湖北未来家园高科技农业有限公司 Harvesting method of Cordyceps taishanensis
CN107125025A (en) * 2017-05-23 2017-09-05 高必红 A kind of cordyceps militaris cultivation method
CN109355202A (en) * 2018-11-22 2019-02-19 陈克飚 A kind of Cordceps militaris cultural method
CN112831383A (en) * 2019-11-25 2021-05-25 湖南金芙农业科技有限公司 Cordyceps militaris culture medium wine and preparation method thereof
CN115568380A (en) * 2022-11-08 2023-01-06 天水众安生物科技有限责任公司 Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof
CN115777444A (en) * 2022-12-07 2023-03-14 天水众安生物科技有限责任公司 Method for cultivating cordyceps militaris by using wheat kernels as culture medium

Also Published As

Publication number Publication date
CN100575475C (en) 2009-12-30

Similar Documents

Publication Publication Date Title
CN100575475C (en) The method of wheat cultivation Cordyceps militaris
CN102100152B (en) Artificial culture method and culture medium for cordyceps militaris sporocarp
CN101897275B (en) Selenium-rich cordyceps culturing method
CN102138437B (en) Artificial cultivation method for Taiwan cordyceps fruiting bodies
CN1029382C (en) Method for artificially cultivating fruiting body of Cordyceps militaris
CN101372671B (en) Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof
CN107197691A (en) A kind of indoor cultivation method for Ganoderma of hickory chick
CN102612985A (en) Production technology for cordyceps militaris mycelium
CN102771314A (en) Method for culturing selenium-enriched cordyceps militaris
CN106472569A (en) A kind of preparation method and application of plant growth substance
CN103299826A (en) Cordyceps militaris method capable of improving content of cordycepin
CN1232632C (en) New strain APC-20 of Paecilomyces cicadae and fementation process for artificial culture
CN105543104A (en) Optimization method of solid culture medium for artificial acclimation and cultivation of wild Isaria cicadae Miquel
CN102895685B (en) Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris
CN104846018A (en) Cordyceps enzyme deep layer liquid fermentation and expanding culture method
CN104350942A (en) Method for preparing cordyceps mycelia through mulberry twig powder and silkworm chrysalis powder
CN102925527A (en) Method for mixing and fermenting flammulina velutipes and lucid ganoderma
CN102318544A (en) A kind of Cordyceps militaris ciltivating process
CN102219567B (en) Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation
CN105296357B (en) A method of aweto liquid fermentation mycelium production is improved by feed supplement
CN107177515A (en) A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation
CN107027516A (en) A kind of selenium-rich Periostracum cicadae, its cultural method and application
CN1338510A (en) Artificial culture method of north cordyceps sporophores and its living product
CN104012263A (en) Plantation method for raising survival rate of Dendrobium huoshanense
CN101182443B (en) Production method of polyporus umbellatus wine

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20091230

Termination date: 20101109