CN115568380A - Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof - Google Patents
Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof Download PDFInfo
- Publication number
- CN115568380A CN115568380A CN202211389131.4A CN202211389131A CN115568380A CN 115568380 A CN115568380 A CN 115568380A CN 202211389131 A CN202211389131 A CN 202211389131A CN 115568380 A CN115568380 A CN 115568380A
- Authority
- CN
- China
- Prior art keywords
- cordyceps militaris
- wheat
- culture medium
- cultivating
- crushed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 123
- 241000209140 Triticum Species 0.000 title claims abstract description 123
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 123
- 239000001963 growth medium Substances 0.000 title claims abstract description 75
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 235000013339 cereals Nutrition 0.000 claims abstract description 56
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000012986 modification Methods 0.000 claims abstract description 15
- 230000004048 modification Effects 0.000 claims abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 238000010411 cooking Methods 0.000 claims abstract description 6
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 238000010894 electron beam technology Methods 0.000 claims description 4
- 230000005251 gamma ray Effects 0.000 claims description 4
- 238000004806 packaging method and process Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 229920002472 Starch Polymers 0.000 abstract description 5
- 230000009467 reduction Effects 0.000 abstract description 5
- 239000008107 starch Substances 0.000 abstract description 5
- 235000019698 starch Nutrition 0.000 abstract description 5
- 238000010025 steaming Methods 0.000 abstract description 5
- 235000015099 wheat brans Nutrition 0.000 abstract description 5
- 108010050181 aleurone Proteins 0.000 abstract description 4
- 238000000855 fermentation Methods 0.000 abstract description 3
- 230000004151 fermentation Effects 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 19
- 238000011081 inoculation Methods 0.000 description 12
- 238000007873 sieving Methods 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 9
- 241000209094 Oryza Species 0.000 description 8
- 235000007164 Oryza sativa Nutrition 0.000 description 8
- 235000009566 rice Nutrition 0.000 description 8
- 238000012258 culturing Methods 0.000 description 7
- 238000001816 cooling Methods 0.000 description 6
- 238000007865 diluting Methods 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 238000003306 harvesting Methods 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 238000012856 packing Methods 0.000 description 6
- 238000005507 spraying Methods 0.000 description 6
- 239000008223 sterile water Substances 0.000 description 6
- 238000005286 illumination Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011068 loading method Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical group [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a broken wheat culture medium for cultivating cordyceps militaris and a preparation method and application thereof, and belongs to the technical field of artificial cultivation of cordyceps militaris. The invention provides a preparation method of a crushed wheat culture medium for cultivating cordyceps militaris, which comprises the following steps: (1) Crushing wheat grains, and performing irradiation modification treatment to obtain crushed wheat; (2) Mixing the broken wheat with water, and steaming and sterilizing to obtain the broken wheat culture medium for cultivating cordyceps militaris. The method takes the broken wheat as the culture medium, fully exposes and gelatinizes the aleurone layer wrapped by the wheat bran, releases more nutrients such as starch, protein and the like, effectively improves the viscosity of the culture medium by irradiation modification treatment and reduction of the cooking sterilization temperature, is beneficial to direct contact utilization of cordyceps militaris hyphae, greatly improves the yield per unit of cordyceps militaris, and has important significance for reduction of cost and improvement of income of solid fermentation of the cordyceps militaris.
Description
Technical Field
The invention relates to the technical field of artificial cultivation of cordyceps militaris, in particular to a broken wheat culture medium for cultivating cordyceps militaris and a preparation method and application thereof.
Background
With the continuous development of cordyceps militaris cultivation technology, most of the cordyceps militaris are cultivated by using grains such as rice and wheat as substrates, and particularly wheat is generally adopted for cultivation in northeast China. At present, wheat is used as a culture medium for cultivating cordyceps militaris, and for example, the research is very much in the patent (publication No. CN109105148A, published Japanese: 20190101)The culture medium takes rice or wheat as a main culture medium, corncobs, wheat bran, wood chips or rice husks accounting for 10-40% of the mass of the rice or the wheat are added, and a mixture with a liquid-liquid ratio of 1: 1.1-1: sterilizing 1.5g/mL nutrient solution to obtain culture medium. The patent (publication number: CN108633624A, publication number: 20181012) discloses a high-yield culture medium for cordyceps sinensis fruiting bodies, which is characterized in that the culture medium is prepared from rice or wheat and nutrient solution according to the mass-volume ratio of 1: 1.2-2, wherein each liter of nutrient solution comprises 10-30 g of carbon source, 5-10 g of nitrogen source and 3-15 g of potassium salt, and finally water is used for constant volume to 1L, and the pH value is 6.0-7.5, the carbon source is sucrose, glucose or soluble starch, the nitrogen source is peptone, beef extract, yeast extract or soybean, and the potassium salt is potassium chloride, potassium nitrate, potassium dihydrogen phosphate or dipotassium hydrogen phosphate and the like. A patent (publication No. CN105315043A, published Japanese: 20160210) discloses a culture medium for culturing Cordyceps militaris, which comprises the following components in percentage by mass: 15 to 25 portions of potato, 1 to 2.5 portions of glucose, 0.05 to 0.15 portion of monopotassium phosphate, 0.05 to 0.15 portion of magnesium sulfate and vitamin B 1 0.10-0.15 part of eggs, 5-10 parts of silkworm chrysalis meal, 10-20 parts of calcium sulfate, 18-25 parts of rice and 20-35 parts of wheat, wherein the rice is rice grains, and the wheat is wheat grains.
However, in the above patent, the wheat grains are used for cultivation, the whole wheat grains are adopted, the back of the wheat grains is fried in the cooking process, part of aleurone layer is exposed, and the rest of cordyceps militaris mycelium cannot be utilized due to the wrapping of wheat bran, and only the nutrient content in the gap part of the wheat grains can be utilized, so that the utilization rate of the matrix is low, the biotransformation rate of the cordyceps militaris mycelium to the wheat grains is not high, and the yield of cordyceps militaris is low. In view of the above, there is an urgent need to develop a crushed wheat culture medium for cultivating cordyceps militaris to increase the utilization rate of the culture medium by cordyceps militaris and increase the yield of cordyceps militaris.
Disclosure of Invention
The invention aims to provide a broken wheat culture medium for cultivating cordyceps militaris and a preparation method and application thereof. The method takes the broken wheat as the culture medium, fully exposes and gelatinizes the aleurone layer wrapped by the wheat bran, releases more nutrients such as starch, protein and the like, effectively improves the viscosity of the culture medium by virtue of irradiation modification treatment and reduction of the cooking sterilization temperature, is beneficial to direct contact utilization of cordyceps militaris hyphae, greatly improves the single yield of cordyceps militaris, and has important significance for reduction of the cost and improvement of the income of solid fermentation of cordyceps militaris.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of a crushed wheat culture medium for cultivating cordyceps militaris, which comprises the following steps:
(1) Crushing wheat grains, and performing irradiation modification treatment to obtain crushed wheat;
(2) Mixing the broken wheat with water, and steaming and sterilizing to obtain the broken wheat culture medium for cultivating cordyceps militaris.
Preferably, the mesh number of the crushed wheat grains is-10 meshes to +30 meshes.
Preferably, the irradiation is electron beam or gamma ray irradiation.
Preferably, the irradiation dose is 2 to 10kGy.
Preferably, during irradiation, the crushed wheat grains are packaged by nylon-polyethylene composite plastic bags, and the packaging thickness is 15-30 cm.
Preferably, the mass ratio of the crushed wheat to the water is 1:1.4 to 2.0.
Preferably, the temperature of the steam sterilization is 85-100 ℃.
Preferably, the time for the cooking sterilization is 30-60 min.
The invention also provides a broken wheat culture medium for cultivating cordyceps militaris, which is prepared by the preparation method.
The invention also provides an application of the broken wheat culture medium for cultivating the cordyceps militaris in improving the yield of the cordyceps militaris.
The invention provides a broken wheat culture medium for cultivating cordyceps militaris and a preparation method and application thereof. The invention adopts the crushed wheat as the culture medium to cultivate the cordyceps, the shape of the crushed wheat is not complete, the aleurone layer wrapped by the wheat bran can be fully exposed and gelatinized, more nutrient substances such as starch, protein and the like are released, the specific surface area of the granules is increased, the nutrient substances available for hyphae are increased, and the direct contact utilization of the cordyceps militaris hyphae is facilitated. However, only broken wheat is adopted to prepare the culture medium, and the nutrient substances dissolved out at high temperature and high pressure are increased, so that the viscosity of the culture medium is greatly increased, a large amount of dissolved components block pore channels, cordyceps militaris hypha is difficult to eat through, and the utilization rate of the matrix is still low. In this regard, the present invention employs irradiation for further processing based on the use of ground wheat. The irradiation modification treatment is to utilize atomic energy to penetrate through an object in the form of electromagnetic waves to activate molecules, ions or free radicals in the irradiated substance to cause the change of the internal structure of the substance. The matrix inside the wheat grain is complex, and free radicals generated in the process of ray irradiation can have certain influence on the internal components and structure of the grain, so that the processing quality, the storage quality and the corresponding food quality of the wheat are changed. The irradiation modification treatment is used for performing modified degradation on the starch, particularly for degrading the molecular weight of amylopectin, and the reduction of the cooking sterilization temperature is combined, so that the problems of overlarge viscosity of a culture medium and difficulty in infiltration of hypha caused by the crushing of the wheat grains are effectively solved, the density of the obtained cordyceps militaris fruiting body is improved, the yield per unit of cordyceps militaris is remarkably improved, the improvement rate is over 36 percent, and the method has important significance for reducing the cost and improving the yield of cordyceps militaris solid fermentation.
Drawings
FIG. 1 is a medium of the present invention, in which FIG. A is the medium prepared in example 1 and FIG. B is the medium prepared in comparative example 1.
FIG. 2 shows the spawn running effect of Cordyceps militaris of the present invention, wherein FIG. A shows the spawn running effect of example 1 on day 6 after inoculation, and FIG. B shows the spawn running effect of comparative example 1 on day 6 after inoculation.
FIG. 3 is the growth state of Cordyceps militaris of the present invention, wherein FIG. A is the growth state of Cordyceps militaris 55 days after inoculation in example 1, and FIG. B is the growth state of Cordyceps militaris 55 days after inoculation in comparative example 1.
Detailed Description
The invention provides a preparation method of a crushed wheat culture medium for cultivating cordyceps militaris, which comprises the following steps:
(1) Crushing wheat grains, and performing irradiation modification treatment to obtain crushed wheat;
(2) Mixing the broken wheat with water, and steaming and sterilizing to obtain the broken wheat culture medium for cultivating cordyceps militaris.
The invention obtains the crushed wheat by crushing wheat grains and then carrying out irradiation modification treatment.
In the invention, the wheat grains are preferably crushed by using a crusher.
In the present invention, the rotation speed of the pulverizer is preferably 400 to 500rpm, and more preferably 460rpm.
In the present invention, the mesh size of the pulverizer is preferably 10 to 12 mesh, and more preferably 12 mesh.
In the invention, the mesh number of the crushed wheat grains is preferably-10 meshes to +30 meshes.
In the present invention, the irradiation is preferably performed with electron beam or γ -ray, and further preferably performed with γ -ray.
In the present invention, the dose of irradiation is preferably 2 to 10kGy, and more preferably 5kGy.
In the invention, during the irradiation, the crushed wheat grains are preferably packaged by a nylon-polyethylene composite plastic bag.
In the present invention, the thickness of the package is preferably 15 to 30cm, and more preferably 20cm.
In the present invention, the mass ratio of the crushed wheat to the water is preferably 1:1.4 to 2.0, more preferably 1:1.8.
in the present invention, the temperature for the retort sterilization is preferably 85 to 100 ℃, and more preferably 90 ℃.
In the present invention, the time for the retort sterilization is preferably 30 to 60min, and more preferably 60min.
In the invention, the loading amount of the broken wheat culture medium for cultivating the cordyceps militaris is preferably 80-120 g of broken wheat/box, and more preferably 100g of broken wheat/box.
After the crushed wheat culture medium for cultivating the cordyceps militaris is prepared, the cordyceps militaris liquid strain is inoculated for cultivation.
In the invention, the cultivation method of the cordyceps militaris preferably comprises the steps of dark cultivation and illumination cultivation.
In the present invention, the temperature of the dark culture is preferably 16 to 19 ℃ and more preferably 18 ℃.
In the present invention, the dark culture time is preferably 3 to 7 days, and more preferably 6 days.
In the present invention, the temperature of the light irradiation culture is preferably 16 to 22 ℃, and more preferably 20 ℃.
In the present invention, the light intensity of the above-mentioned light culture is preferably 50 to 1000Lux, and more preferably 800Lux.
In the invention, the cordyceps militaris is preferably harvested 50-60 days after inoculation, and is further preferably harvested 55 days after inoculation.
The invention also provides a crushed wheat culture medium for cultivating cordyceps militaris, which is prepared by the preparation method.
The invention also provides an application of the broken wheat culture medium for cultivating the cordyceps militaris in improving the yield of the cordyceps militaris.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
The invention provides a preparation method of a crushed wheat culture medium for cultivating cordyceps militaris, which comprises the following specific operations:
sieving wheat grains to remove impurities, and crushing the wheat grains by using a crusher with a sieve mesh size of 12 meshes at a set rotating speed of 460rpm. Sieving the crushed wheat grains, and sieving and collecting the wheat grains between-10 meshes and +30 meshes. The yield of the obtained wheat grains is 88.8%. Packing the wheat grains with a nylon-polyethylene composite plastic bag, wherein the packing thickness is 20cm, and performing irradiation modification treatment on the wheat grains by adopting gamma-rays with the dose of 5kGy to obtain the crushed wheat. Mixing broken wheat with water according to the proportion of 1:1.8, steaming and sterilizing at 90 ℃ for 60min, and cooling and forming to obtain the crushed wheat culture medium for cultivating the cordyceps militaris, wherein the content of the culture medium is 100g of crushed wheat per box.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared crushed wheat culture medium, and inoculating 8mL of Cordyceps militaris liquid strain per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 6 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting 55 days after inoculation, drying the harvested cordyceps militaris fruiting bodies at 60 ℃ for 24h, and calculating the output of cordyceps militaris.
Example 2
The invention provides a preparation method of a crushed wheat culture medium for cultivating cordyceps militaris, which comprises the following specific operations:
sieving wheat grains to remove impurities, and crushing the wheat grains by using a crusher with a sieve mesh aperture of 10 meshes at a set rotating speed of 420 rpm. Sieving the crushed wheat grains, and sieving and collecting the wheat grains with a grain size of-10 meshes to +30 meshes. The yield of the obtained wheat grains is 84.6%. Packing the wheat grains with a nylon-polyethylene composite plastic bag, wherein the packing thickness is 20cm, and performing irradiation modification treatment on the wheat grains by adopting an electron beam with the dose of 10kGy to obtain the crushed wheat. Mixing broken wheat with water according to the proportion of 1:2.0, stewing and sterilizing at 95 ℃ for 30min, cooling and forming to obtain the broken wheat culture medium for cultivating the cordyceps militaris, wherein the filling amount of the culture medium is 100g of broken wheat per box.
Diluting 10g/L Cordyceps militaris strain with sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, and uniformly spraying Cordyceps militaris liquid strain onto the surface of the prepared crushed wheat culture medium, wherein each bottle is inoculated with 10mL of Cordyceps militaris liquid strain. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 5 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, 1000Lux light was applied and cultivation management was performed at 18 ℃. Harvesting on 60 days after inoculation, drying the harvested Cordyceps militaris fruiting body at 60 deg.C for 24h, and calculating the output of Cordyceps militaris.
Example 3
The invention provides a preparation method of a crushed wheat culture medium for cultivating cordyceps militaris, which comprises the following specific operations:
sieving wheat grains to remove impurities, and crushing the wheat grains by using a crusher with a sieve mesh of 10 meshes at a set rotating speed of 460rpm. Sieving the crushed wheat grains, and sieving and collecting the wheat grains with a grain size of-10 meshes to +30 meshes. The yield of the obtained wheat grains is 95.0%. Packing the wheat grains with nylon-polyethylene composite plastic bags, wherein the packing thickness is 20cm, and performing irradiation modification treatment on the wheat grains by adopting gamma-rays with the dose of 2kGy to obtain the crushed wheat. Mixing broken wheat with water according to the proportion of 1:1.5, stewing and sterilizing at 100 ℃ for 40min, cooling and forming to obtain a broken wheat culture medium for cultivating cordyceps militaris, wherein the content of the culture medium is 100g of broken wheat per box.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared crushed wheat culture medium, and inoculating 8mL of Cordyceps militaris liquid strain per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 6 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 18 ℃ under illumination of 800Lux. Harvesting at 62 days after inoculation, drying the harvested Cordyceps militaris fruiting body at 60 deg.C for 24h, and calculating the output of Cordyceps militaris.
Comparative example 1
Sieving the complete wheat grains to remove impurities, and mixing the wheat grains with water according to the ratio of 1:1.8, stewing and sterilizing at 121 ℃ for 30min, cooling and forming to obtain the culture medium for cultivating the cordyceps militaris, wherein the loading amount of the culture medium is 100g of wheat grains/box.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying Cordyceps militaris liquid strain onto the surface of the prepared culture medium, and inoculating 8mL each bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 6 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting 55 days after inoculation, drying the harvested cordyceps militaris fruiting bodies at 60 ℃ for 24h, and calculating the output of cordyceps militaris.
Comparative example 2
Sieving the complete wheat grains to remove impurities, and mixing the wheat grains with water according to the ratio of 1:2.0, stewing and sterilizing at 123 ℃ for 30min, cooling and forming to obtain the culture medium for cultivating the cordyceps militaris, wherein the content of the culture medium is 100g of wheat grains/box.
Diluting 10g/L Cordyceps militaris strain with sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, and uniformly spraying Cordyceps militaris liquid strain onto the surface of the prepared crushed wheat culture medium, wherein each bottle is inoculated with 10mL of Cordyceps militaris liquid strain. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 5 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, 1000Lux light was applied and cultivation management was performed at 18 ℃. Harvesting on 60 days after inoculation, drying the harvested cordyceps militaris fruiting bodies at 60 ℃ for 24h, and calculating the output of cordyceps militaris.
Comparative example 3
Sieving complete wheat grains to remove impurities, and mixing the wheat grains with water according to the weight ratio of 1:1.5, steaming and sterilizing at 122 ℃ for 30min, cooling and forming to obtain the culture medium for cultivating the cordyceps militaris, wherein the loading amount of the culture medium is 100g of wheat grains/box.
Diluting 10g/L Cordyceps militaris strain with sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, spraying Cordyceps militaris liquid strain onto the surface of the prepared crushed wheat culture medium, and inoculating 8mL of Cordyceps militaris liquid strain per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 6 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 18 ℃ under illumination of 800Lux. Harvesting at 62 days after inoculation, drying the harvested Cordyceps militaris fruiting body at 60 deg.C for 24h, and calculating the output of Cordyceps militaris.
The unit yield of cordyceps militaris in each example and comparative example is counted, and the result is shown in table 1.
TABLE 1 Individual yield of Cordyceps militaris in each example and comparative example
As can be seen from Table 1, the single-yield of the Cordyceps militaris can be increased by more than 36% by crushing the wheat grains, performing irradiation modification treatment, and reducing the sterilization temperature to prepare the culture medium, thereby significantly increasing the yield of the Cordyceps militaris.
Comparing the culture medium, the cordyceps militaris spawn running effect and the growth state in the embodiment 1 with those in the comparative example 1, as shown in fig. 1-3, it can be seen that the culture medium prepared by the method of the invention can enable cordyceps militaris spawn running to be denser, the growth speed to be faster and the number of sporophores to be larger.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A preparation method of a crushed wheat culture medium for cultivating cordyceps militaris is characterized by comprising the following steps:
(1) Crushing wheat grains, and performing irradiation modification treatment to obtain crushed wheat;
(2) And mixing the crushed wheat with water, and performing cooking sterilization to obtain the crushed wheat culture medium for cultivating the cordyceps militaris.
2. The preparation method of claim 1, wherein the crushed wheat grains have a mesh size of-10 meshes to +30 meshes.
3. The method according to claim 2, wherein the irradiation is performed by electron beam irradiation or gamma-ray irradiation.
4. The production method according to claim 3, wherein the irradiation dose is 2 to 10kGy.
5. The preparation method according to claim 4, wherein during the irradiation, the crushed wheat grains are packaged by a nylon-polyethylene composite plastic bag, and the packaging thickness is 15-30 cm.
6. The method according to claim 5, wherein the mass ratio of the crushed wheat to the water is 1:1.4 to 2.0.
7. The method according to claim 6, wherein the temperature for the retort sterilization is 85 to 100 ℃.
8. The method of claim 7, wherein the retort sterilization is performed for 30 to 60min.
9. A crushed wheat culture medium for cultivating cordyceps militaris, which is prepared by the preparation method of any one of claims 1 to 8.
10. An application of the crushed wheat culture medium for cultivating cordyceps militaris in claim 9 in improving the yield of cordyceps militaris.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211389131.4A CN115568380A (en) | 2022-11-08 | 2022-11-08 | Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211389131.4A CN115568380A (en) | 2022-11-08 | 2022-11-08 | Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115568380A true CN115568380A (en) | 2023-01-06 |
Family
ID=84589119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211389131.4A Pending CN115568380A (en) | 2022-11-08 | 2022-11-08 | Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115568380A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101173221A (en) * | 2007-11-09 | 2008-05-07 | 乔善成 | Wheat cultivation Cordyceps militaris method |
| KR20090126529A (en) * | 2008-06-04 | 2009-12-09 | 충남대학교산학협력단 | Mushroom culture medium irrediated by electron beam and preparation method thereof |
| CN105801219A (en) * | 2016-04-27 | 2016-07-27 | 许五妮 | Method for preparing mushroom culture medium by using sheep manure |
| CN108207491A (en) * | 2018-01-13 | 2018-06-29 | 蚌埠学院 | A kind of method using wheat embryo cultivation fruiting bodies of cordyceps militaris |
| CN109105148A (en) * | 2018-08-21 | 2019-01-01 | 福建农林大学 | A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method |
| CN114711086A (en) * | 2022-03-15 | 2022-07-08 | 江苏隆力奇生物科技股份有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp by using wheat |
| CN114836289A (en) * | 2022-04-06 | 2022-08-02 | 湖北菇菇坊生态农业有限公司 | Preparation method of cordyceps militaris wine with high cordycepin and pentostatin content |
-
2022
- 2022-11-08 CN CN202211389131.4A patent/CN115568380A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101173221A (en) * | 2007-11-09 | 2008-05-07 | 乔善成 | Wheat cultivation Cordyceps militaris method |
| KR20090126529A (en) * | 2008-06-04 | 2009-12-09 | 충남대학교산학협력단 | Mushroom culture medium irrediated by electron beam and preparation method thereof |
| CN105801219A (en) * | 2016-04-27 | 2016-07-27 | 许五妮 | Method for preparing mushroom culture medium by using sheep manure |
| CN108207491A (en) * | 2018-01-13 | 2018-06-29 | 蚌埠学院 | A kind of method using wheat embryo cultivation fruiting bodies of cordyceps militaris |
| CN109105148A (en) * | 2018-08-21 | 2019-01-01 | 福建农林大学 | A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method |
| CN114711086A (en) * | 2022-03-15 | 2022-07-08 | 江苏隆力奇生物科技股份有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp by using wheat |
| CN114836289A (en) * | 2022-04-06 | 2022-08-02 | 湖北菇菇坊生态农业有限公司 | Preparation method of cordyceps militaris wine with high cordycepin and pentostatin content |
Non-Patent Citations (4)
| Title |
|---|
| 冉晓燕等: "双梭孢虫草的人工栽培及其子实体中虫草素、腺苷和多糖的测定", 贵州师范学院学报, vol. 35, no. 6, 28 September 2019 (2019-09-28), pages 31 - 34 * |
| 史淑菊: "《发酵食品加工》", 28 February 2021, 中国农业大学出版社, pages: 176 - 177 * |
| 李学如等: "《发酵工艺原理与技术》", 31 August 2014, 华中科技大学出版社, pages: 74 - 76 * |
| 马振瀛: "《实用防霉技术》", 31 March 1987, 上海科学技术出版社, pages: 190 - 193 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103168617A (en) | Cut-log cultivation method of polyporusrhinocerus cooke | |
| CN105660191B (en) | A kind of cultural method of crow sesame fructification | |
| CN104969773B (en) | The method for obtaining Cordyceps militaris fermentate or fructification using sweet potato dregs fermentation | |
| CN106434373A (en) | High-density fermentation medium formula of sparassis crispa and pharmaceutical grade glucan preparation method of high-density fermentation medium formula | |
| CN104478546B (en) | A kind of method utilizing Vitex chinensis Mill. bits to cultivate hedgehog hydnum | |
| CN110301291B (en) | Method for cultivating morchella strain | |
| CN102845225A (en) | Hypsizygus marmoreus liquid strain fermenting technique | |
| CN112021073A (en) | Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof | |
| CN105018447B (en) | A kind of method using fungus Trichoderma High Cellulase Production | |
| CN110923281B (en) | Edible fungus polysaccharide extraction method, edible fungus polysaccharide and edible fungus beverage | |
| CN108718915A (en) | Improve the culture medium and cultural method of pleurotus edible fungus yield | |
| CN104641944A (en) | Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium | |
| CN108770592B (en) | Culture medium for pholiota citrina culture and pholiota citrina culture method | |
| CN104303845B (en) | A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree | |
| CN108175040B (en) | Preparation method of millet oat antrodia camphorata mycoplasm health food | |
| CN108795819A (en) | A kind of complex microorganism culture and its application in producing carotenoid | |
| Dulay et al. | Optimal growth conditions for basidiospore germination and morphogenesis of Philippine wild strain of Lentinus tigrinus (Bull.) Fr | |
| CN115568380A (en) | Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof | |
| KR100361681B1 (en) | Method for cultivating phellinus linteus with using wood medic | |
| CN113079951B (en) | Preparation method of wide-matrix adaptive edible fungus liquid strain | |
| CN109328865B (en) | Preparation method of cordyceps militaris and cordyceps militaris prepared by same | |
| KR101063754B1 (en) | Cultivation method for Mycoleptodonoides aitchisonii | |
| CN112314330A (en) | Method for culturing Roberts' cordyceps sinensis | |
| CN116569786B (en) | Preparation method of edible fungus solid mycelium capable of being directly eaten | |
| CN115769767B (en) | Application of sarcandra glabra wood chips combined with pennisetum hydridum in improving yield and quality of edible fungi, culture medium, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |