CN101372671B - Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof - Google Patents
Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof Download PDFInfo
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- CN101372671B CN101372671B CN2008101573819A CN200810157381A CN101372671B CN 101372671 B CN101372671 B CN 101372671B CN 2008101573819 A CN2008101573819 A CN 2008101573819A CN 200810157381 A CN200810157381 A CN 200810157381A CN 101372671 B CN101372671 B CN 101372671B
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Abstract
The invention discloses an artificial culture method of a zinc-rich cordyceps militaris fruit body and a culture medium thereof. The culture medium comprises the following components by weight portions: 40-65 portions of rice, 0.5-2.0 portions of a yeast extract, 60-85 portions of water and 0.01-1.0 portion of zinc sulfate with the natural PH value. The artificial culture method comprises the following steps: preparation of the culture medium, canning and sterilization; cooling and inoculation; culture; collection of the fruit body; drying and granulation. The method has the advantages of simple operation, short culture period, high yield of the fruit body, no pollution, no waste and zinc supplement function, and the method is suitable for industrialized production.
Description
Technical field:
The present invention relates to a kind of artificial culture method and substratum thereof of zinc-enriched Chinese caterpillar fungus sporophore, belong to the pharmacognosy technical field.
Background technology:
Cordyceps militaris (L.) Link. has another name called Cordyceps militaris (L.) Link., belongs to Ascomycotina gang pyrenomycetes Sphaerials Clavicipitaceae Cordyceps, is one of China's rare traditional Chinese medicine.Modern pharmacy studies show that component contents such as containing ucleosides, polyose, cordycepin, sterols in the Cordyceps militaris (L.) Link. is basic identical with Cordyceps sinensis, to immunity system, disease such as neural system and cardiovascular systems has result of treatment, and have antitumor and antitumous effect, be Cordyceps sinensis ideal substitute, vast potential for future development is arranged.
Zinc is a kind of of trace element, the intravital content of people and every day required intake all seldom, but the generation to the sexual development of body, sexual function, sexual cell can be played very important effect, so the title of " spark of life " and " marriage is harmonious plain " is arranged.The normal zinc content of human body is the 2-3 gram.All have the zinc of denier to distribute in the overwhelming majority tissue, wherein liver, muscle and bone content are higher.Zinc is the main component of tens of kinds of enzymes in the body; Zinc is also relevant with brain development and intelligence.In the middle of the testis of human body, prostate gland, the seminal fluid, all contain the zinc of high density.When the content of zinc in the human body lacked, sexual function can be therefore and low, and synthetic Testosterone enzyme gets muddled, and the man will cause impotence and the acne of growing on the face.Zinc has special effect to exciting sperm motility, and scarce zinc can cause the decline of motility of sperm.Be in for a long time to lack the zinc state and fail and in time replenish, can occur that sperm quantity obviously reduces, testicular atrophy, cause sterile at last.
Though zinc is trace element in human body, acts on very big.Child lacks zinc, with directly influencing the growth of physique and brain, also phenomenons such as apocleisis, hypoimmunity can occur.Zinc can improve human immune system's susceptibility, can directly suppress the activity of virus simultaneously, thereby strengthens the human body resistance against diseases; Also can promote brain albumen synthetic, help neural growth and perfect.Zinc can promote the taste bud cells whose development, strengthens child's appetite, improves the situation of child's apocleisis, partial eclipse, guarantees comprehensive absorption of nutrition.
World Health Organization's regulation, the intake of per day for adults zinc is 15 milligrams, and China is 12～15mg to adult day recommended amounts, and the intake of China man zinc is approximately just about 9 milligrams.Dietary supplements can day dosing be 1/3～1/2 of a day recommended amounts, promptly can replenish 4mg～7.5mg by the mode beyond the food every day.
Both at home and abroad at present about zinc-enriched Chinese caterpillar fungus sporophore product and the not corresponding report of cultural method thereof.
Summary of the invention:
The objective of the invention is to overcome the deficiency of above-mentioned prior art and provide a kind of simple to operate, culture cycle is short, sporophore productive rate height, pollution-free no waste, have the zinc supplementation function, can carry out the artificial culture method and the substratum thereof of a kind of zinc-enriched Chinese caterpillar fungus sporophore of batch production production.
Purpose of the present invention can reach by following measure: a kind of substratum that is used to cultivate the zinc-enriched Chinese caterpillar fungus sporophore, it is characterized in that its recipe ingredient is by weight: 40-65 parts in rice, 0.5-2.0 parts of yeast extracts, 60-85 parts in water, 0.01-1.0 parts in zinc sulfate.
In order further to realize purpose of the present invention, its recipe ingredient is by weight: 50 parts in rice, 1.0 parts of yeast extracts, 75 parts in water, 0.5 part in zinc sulfate.
A kind of cultural method of zinc-enriched Chinese caterpillar fungus sporophore is characterized in that comprising medium preparation, sterilization, and inoculation is cultivated, separates, pulverising step, concrete processing step is:
(1) rich zinc medium preparation:
Its recipe ingredient is by weight: 40-65 parts in rice, 0.5-2.0 parts of yeast extracts, 60-85 parts in water, 0.01-1.0 parts in zinc sulfate, natural pH value;
(2) substratum packing, sterilization:
Prepared culture medium is sub-packed in the Cans, seals, under 120-130 ℃ of conditions, sterilized 18-30 minutes;
(3) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted Cordyceps militaris spawn;
(4) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(5) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(6) oven dry, granulate:
Place 50-80 ℃ of air stream drying casees to dry to constant weight sporophore, pulverize the back and cross 60-120 mesh sieves, alcohol granulation, 50-80 ℃ of dryings puts encapsulatedly, use to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore capsule product.
In order further to realize purpose of the present invention, the Cordyceps militaris spawn that inserts in the described step 3 is the Cordyceps militaris spawn of solid culture.
In order further to realize purpose of the present invention, the Cordyceps militaris spawn that inserts in the described step 3 is the Cordyceps militaris (L.) Link. liquid spawn, and this Cordyceps militaris (L.) Link. liquid spawn is cultivated by test tube slant spawn culture and triangular flask liquid seeds and forms, and concrete processing step is:
The test tube slant spawn culture:
The preparation substratum: its weight ratio ingredient is: glucose 1.0%-3.0%, and peptone 0.5%-1.0%, yeast extract 0.1%-1.0%, agar powder 1.0%-2.0%, water surplus, its pH value is 5.0-7.0; Cordyceps militaris spawn is inserted the test tube slant under aseptic condition, 15～30 ℃ of dark cultivations 5-10 days;
The triangular flask liquid seeds is cultivated:
The preparation substratum: its weight ratio ingredient is: glucose 1.0%-3.0%, peptone 0.5%-1.0%, yeast extract 0.1%-1.0%, water surplus, its pH value is 5.0-7.0, the prepared culture medium branch is packed in the Erlenmeyer flask, seal the back and under 120～130 ℃ of conditions, sterilized 18～30 minutes, take out substratum, naturally cool to 20 ℃～30 ℃, under aseptic condition, insert the slant strains after cultivating, cultivated 5 days～10 days for 15～30 ℃.
The present invention can produce following positively effect and obvious improvement compared with the prior art: the applicant passes through the research of Cordyccps-militaris-(L.)-link. Sporophore to the zinc ion concentration rule, recognize the ability height of the enrichment zinc of Cordyccps-militaris-(L.)-link. Sporophore, culture cycle of the present invention is short, sporophore productive rate height, pollution-free no waste has the zinc supplementation function.The present invention adopts the zinc-enriched Chinese caterpillar fungus sporophore of specific substratum and cultural method cultivation, can increase the effect of product.And rich zinc sporophore adds inorganic zinc in the process of cultivating, by Cordyceps militaris (L.) Link. fungus absorption and conversion to zinc element in process of growth, reduced zinc to GI stimulation, makes the zinc utilization that can more efficient, more safely be absorbed by the body.
Pharmacological evaluation and clinical experiment data show, Cordyceps militaris (L.) Link. is taken 3g every day can significant immunoregulation effect.In the culturing process of Cordyccps-militaris-(L.)-link. Sporophore, not only raw material is easy to get and cost is low to adopt substratum of the present invention, and zinc-enriched Chinese caterpillar fungus productive rate height, reach rich zinc 8 to 11 grams of every hectogram, exceed 8000 to 10000 times than the rich zinc rate of natural Cordyceps militaris (L.) Link., can make the Cordyceps militaris (L.) Link. product except the pharmacological action of performance Chinese caterpillar fungus itself, can also play the effect that replenishes zinc.
The present invention can control the zinc content in the product by the zinc add-on in the design substratum, obtains the Cordyccps-militaris-(L.)-link. Sporophore of needed zinc content.
Embodiment:
Below the specific embodiment of the present invention is elaborated:
Embodiment 1:
(1) bacterial classification is prepared:
The cryopreservation Cordyceps militaris spawn: Cordyceps militaris spawn Cordyceps militaris (L.) Link. 5.701 derives from Chinese common micro-organisms culture presevation administrative center.
Cordyceps militaris (L.) Link. liquid spawn: cultivate by following processing step:
(1) test tube slant spawn culture (activation):
The preparation substratum: glucose 10g, peptone 10g, yeast extract 10g, agar powder 10g, water 960g, its pH value is 5.0, is sub-packed in the test tube; The cryopreservation bacterial classification is inserted the test tube slant under aseptic condition, 15～30 ℃ of dark cultivations 5-10 days.
(2) the triangular flask liquid seeds is cultivated:
Preparation substratum: glucose 10g, peptone 10g, yeast extract 10g, water 970g, its pH value is 5.0, and the prepared culture medium branch is packed in the 500ml Erlenmeyer flask, every bottled 200ml, seal the back and under 120～130 ℃ of conditions, sterilized 18～30 minutes, take out substratum, naturally cool to 20～30 ℃; Every bottle graft is gone into the cultured test tube liquid spawn of 10ml-20ml under aseptic condition, and 15～30 ℃, mixing speed 120rpm cultivated 5 days～10 days.
(2) rich zinc medium preparation:
Its recipe ingredient is by weight: rice 40g, yeast extract 0.5g, water 60g, zinc sulfate 0.01g, natural pH value;
(3) substratum packing, sterilization:
Prepared culture medium is sub-packed in the 500ml Cans, and edible mushrooms special-purpose sealing film seals, and sterilizes 18-30 minutes under 120-130 ℃ of conditions;
(4) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted Cordyceps militaris spawn; Every bottle graft is gone into the cultured Cordyceps militaris (L.) Link. liquid spawn of 2-10ml;
(5) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(6) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(7) oven dry, granulate:
Place 50-80 ℃ of air stream drying casees to dry to constant weight sporophore, pulverize the back and cross 60-120 mesh sieves, alcohol granulation, 50-80 ℃ of dryings puts encapsulatedly, use to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore product.
Embodiment 2:
(1) bacterial classification is prepared:
Cordyceps militaris spawn: Cordyceps militaris (L.) Link. 5.701 derives from Chinese common micro-organisms culture presevation administrative center.
(2) rich zinc medium preparation:
Its recipe ingredient is by weight: rice 65g, yeast extract 2.0g, water 85g, zinc sulfate 1g, natural pH value;
(3) substratum packing, sterilization:
Prepared culture medium is sub-packed in the 500ml Cans, and edible mushrooms special-purpose sealing film seals, and sterilizes 18-30 minutes under 120-130 ℃ of conditions;
(4) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted the Cordyceps militaris spawn of solid culture;
(5) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(6) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(7) oven dry, granulate:
Place 50-80 ℃ of air stream drying casees to dry to constant weight sporophore, pulverize the back and cross 60-120 mesh sieves, alcohol granulation, 50-80 ℃ of dryings puts encapsulatedly, use to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore product.
Embodiment 3:
(1) bacterial classification is prepared:
Cordyceps militaris spawn: Cordyceps militaris (L.) Link. 5.701 derives from Chinese common micro-organisms culture presevation administrative center.
Cordyceps militaris (L.) Link. liquid spawn: cultivate by following processing step:
(1) test tube slant spawn culture (activation):
The preparation substratum: glucose 20g, peptone 6g, yeast extract 9g, agar powder 15g, water 950g, its pH value is 6.0, is sub-packed in the test tube; The cryopreservation bacterial classification is inserted the test tube slant under aseptic condition, 15～30 ℃ of dark cultivations 5-10 days.
(2) the triangular flask liquid seeds is cultivated:
Preparation substratum: glucose 20g, peptone 6g, yeast extract 9g, water 965g, its pH value is 6.0, and the prepared culture medium branch is packed in the 500ml Erlenmeyer flask, every bottled 200ml, seal the back and under 120～130 ℃ of conditions, sterilized 18～30 minutes, take out substratum, naturally cool to 20～30 ℃; Every bottle graft is gone into the cultured test tube liquid spawn of 10ml-20ml under aseptic condition, and 15～30 ℃, mixing speed 120rpm cultivated 5 days～10 days.
(2) rich zinc medium preparation:
Its recipe ingredient is by weight: rice 45g, yeast extract 1.0g, water 65g part, zinc sulfate 0.5g, natural pH value;
(3) substratum packing, sterilization:
Prepared culture medium is sub-packed in the 500ml Cans, and edible mushrooms special-purpose sealing film seals, and sterilizes 18-30 minutes under 120-130 ℃ of conditions;
(4) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted Cordyceps militaris spawn; Every bottle graft is gone into the cultured Cordyceps militaris (L.) Link. liquid spawn of 2-10ml;
(5) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(6) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(7) oven dry, granulate:
Place 50-80 ℃ of air stream drying casees to dry to constant weight sporophore, pulverize the back and cross 60-120 mesh sieves, alcohol granulation, 50-80 ℃ of dryings puts encapsulatedly, use to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore product.
Embodiment 4:
(1) bacterial classification is prepared:
Cordyceps militaris spawn: Cordyceps militaris (L.) Link. 5.701 derives from Chinese common micro-organisms culture presevation administrative center.
(2) rich zinc medium preparation:
Its recipe ingredient is by weight: rice 60g, yeast extract 1.5g, water 80g, zinc sulfate 0.2g, natural pH value;
(3) substratum packing, sterilization:
Prepared culture medium is sub-packed in the 500ml Cans, and edible mushrooms special-purpose sealing film seals, and sterilizes 18-30 minutes under 120-130 ℃ of conditions;
(4) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted Cordyceps militaris spawn;
(5) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(6) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(7) oven dry, granulate:
Place 50-80 ℃ of air stream drying casees to dry to constant weight sporophore, pulverize the back and cross 60-120 mesh sieves, alcohol granulation, 50-80 ℃ of dryings puts encapsulatedly, use to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore product.
Embodiment 5:
(1) bacterial classification is prepared:
The cryopreservation Cordyceps militaris spawn: Cordyceps militaris spawn Cordyceps militaris (L.) Link. 5.701 derives from Chinese common micro-organisms culture presevation administrative center.
Cordyceps militaris (L.) Link. liquid spawn: cultivate by following processing step:
(1) test tube slant spawn culture (activation):
The preparation substratum: glucose 30g, peptone 5g, yeast extract 5g, agar powder 20g, water 940g, its pH value is 7.5, is sub-packed in the test tube; The cryopreservation bacterial classification is inserted the test tube slant under aseptic condition, 15～30 ℃ of dark cultivations 5-10 days.
(2) the triangular flask liquid seeds is cultivated:
Preparation substratum: glucose 30g, peptone 5g, yeast extract 5g, water 960g, its pH value is 7.5, and the prepared culture medium branch is packed in the 500ml Erlenmeyer flask, every bottled 200ml, seal the back and under 120～130 ℃ of conditions, sterilized 18～30 minutes, take out substratum, naturally cool to 20～30 ℃; Every bottle graft is gone into the cultured test tube liquid spawn of 10ml-20ml under aseptic condition, and 15～30 ℃, mixing speed 120rpm cultivated 5 days～10 days.
(2) rich zinc medium preparation:
Its recipe ingredient is by weight: rice 50g, yeast extract 1.5g, water 70g, zinc sulfate 0.5g, natural pH value;
(3) substratum packing, sterilization:
Prepared culture medium is sub-packed in the 500ml Cans, and edible mushrooms special-purpose sealing film seals, and sterilizes 18-30 minutes under 120-130 ℃ of conditions;
(4) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted Cordyceps militaris spawn; Every bottle graft is gone into the cultured above-mentioned Cordyceps militaris (L.) Link. liquid spawn of 2-10ml;
(5) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(6) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(7) oven dry, granulate:
Place 50-80 ℃ of air stream drying casees to dry to constant weight sporophore, pulverize the back and cross 60-120 mesh sieves, alcohol granulation, 50-80 ℃ of dryings puts encapsulatedly, use to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore product.
Claims (5)
1. a substratum that is used to cultivate the zinc-enriched Chinese caterpillar fungus sporophore is characterized in that its recipe ingredient is by weight: 4065 parts in rice, yeast extract 0.5-2.0 part, water 60-85 part, zinc sulfate 0.01-1.0 part.
2. a kind of substratum that is used to cultivate the zinc-enriched Chinese caterpillar fungus sporophore according to claim 1 is characterized in that its recipe ingredient is by weight: 50 parts in rice, 1.0 parts of yeast extracts, 75 parts in water, 0.5 part in zinc sulfate.
3. the cultural method of a zinc-enriched Chinese caterpillar fungus sporophore is characterized in that comprising medium preparation, sterilization, and inoculation is cultivated, separates, pulverising step, concrete processing step is:
(1) rich zinc medium preparation:
Its recipe ingredient is by weight: rice 40-65 part, yeast extract 0.5-2.0 part, water 60-85 part, zinc sulfate 0.01-1.0 part, natural pH value;
(2) substratum packing, sterilization:
Prepared culture medium is sub-packed in the Cans, seals, sterilization is 18-30 minute under 120-130 ℃ of condition;
(3) cooling, inoculation:
Gone out and taken out substratum behind the bacterium, naturally cooled to 20 ℃-30 ℃, under aseptic condition, inserted Cordyceps militaris spawn;
(4) cultivate:
The zinc-enriched Chinese caterpillar fungus substratum that connects kind is placed in the constant incubator, cultivated 8-12 days for 20-30 ℃, after mycelia fully grows, transfer to 18-20 ℃, relative humidity 85%-95%, 11-13 hour every day and cultivated 50-90 days under the scattered light condition;
(5) sporophore of gathering:
With the sporophore that grows tweezers extraction, remove the substratum that sporophore connects;
(6) oven dry, granulate:
Place 50-80 ℃ of air stream drying case to dry to constant weight sporophore, pulverize the back and cross the 60-120 mesh sieve, alcohol granulation, 50-80 ℃ of drying put encapsulatedly, uses to pack behind cobalt 60 irradiation-sterilizes promptly to get zinc-enriched Chinese caterpillar fungus sporophore capsule product.
4. the cultural method of a kind of zinc-enriched Chinese caterpillar fungus sporophore according to claim 3 is characterized in that the Cordyceps militaris spawn that inserts in the described step 3 is the Cordyceps militaris spawn of solid culture.
5. the cultural method of a kind of zinc-enriched Chinese caterpillar fungus sporophore according to claim 3, it is characterized in that the Cordyceps militaris spawn that inserts in the described step 3 is the Cordyceps militaris (L.) Link. liquid spawn, this Cordyceps militaris (L.) Link. liquid spawn is cultivated by test tube slant spawn culture and triangular flask liquid seeds and forms, and concrete processing step is:
The test tube slant spawn culture:
The preparation substratum: its weight ratio ingredient is: glucose 1.0%-3.0%, and peptone 0.5%-1.0%, yeast extract 0.1%-1.0%, agar powder 1.0%-2.0%, water surplus, its pH value is 5.0-7.0; Cordyceps militaris spawn is inserted the test tube slant under aseptic condition, 15～30 ℃ of dark cultivations 5-10 days;
The triangular flask liquid seeds is cultivated:
The preparation substratum: its weight ratio ingredient is: glucose 1.0%-3.0%, peptone 0.5%-1.0%, yeast extract 0.1%-1.0%, water surplus, its pH value is 5.0-7.0, the prepared culture medium branch is packed in the Erlenmeyer flask, seal the back and under 120～130 ℃ of conditions, sterilized 18～30 minutes, take out substratum, naturally cool to 20 ℃～30 ℃, under aseptic condition, insert the slant strains after cultivating, cultivated 5 days～10 days for 15～30 ℃.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101659578B (en) * | 2009-09-18 | 2012-10-17 | 鲁东大学 | Artificial culture method of Kyushu cordyceps sinensis and culture medium thereof |
| CN102440141B (en) * | 2010-10-08 | 2012-12-26 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN102100152B (en) * | 2010-11-17 | 2012-05-23 | 鲁东大学 | Artificial culture method and culture medium for cordyceps militaris sporocarp |
| CN102172273B (en) * | 2011-03-09 | 2013-02-20 | 王永显 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
| CN104871830B (en) * | 2015-06-15 | 2017-03-15 | 鲁东大学 | With bean worm as the method for carrier-planting Cordyceps militaris (L.) Link. |
| CN105733957A (en) * | 2016-03-01 | 2016-07-06 | 鲁东大学 | Method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains |
| CN106381271A (en) * | 2016-08-16 | 2017-02-08 | 日照市大地芝草生物科技有限公司 | Making method for selenium-rich Cordyceps militaris |
| CN107182544A (en) * | 2016-10-27 | 2017-09-22 | 镇江市农业科学技术实业公司 | A kind of Phellinus fructification rough machining method |
| CN106718036A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris moves cultivating technique |
| CN107502554A (en) * | 2017-09-19 | 2017-12-22 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris spawn high yield cultural method |
| CN110547141A (en) * | 2019-09-30 | 2019-12-10 | 江苏康能生物工程股份有限公司 | Cultivation method for increasing polysaccharide content in cordyceps militaris sporocarp |
| CN112868779A (en) * | 2021-02-25 | 2021-06-01 | 李春桐 | Health-care yoghourt containing active ingredients of bacteria |
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