CN104012263A - Plantation method for raising survival rate of Dendrobium huoshanense - Google Patents
Plantation method for raising survival rate of Dendrobium huoshanense Download PDFInfo
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- CN104012263A CN104012263A CN201310061663.XA CN201310061663A CN104012263A CN 104012263 A CN104012263 A CN 104012263A CN 201310061663 A CN201310061663 A CN 201310061663A CN 104012263 A CN104012263 A CN 104012263A
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Abstract
The invention provides a plantation method for raising survival rate of Dendrobium huoshanense. Specifically, a micro-ecological bacteria liquid is prepared from a plurality of stock solutions, components of which contain heterogenous mycoprotein, a beauveria bassiana stock solution, a photosynthetic bacteria stock solution and biological organic bacterial manure (nutritive water). The bacterial liquid can be used as a bactericidal and insecticidal medicament as well as an organic fertilizer, and only needs to be sprayed at fixed period. By adjusting and controlling a micro-ecological environment of microorganisms and spraying the micro-ecological bacteria liquid, an effective microbial community in microflora inside a plant can become a dominant bacterial community. Effects of the effective microbial community, such as nutrient competition and the like, are fully performed; the number of harmful microbial communities is controlled below economic harm level; a maladjusted microecosystem is recovered and balanced once again; and reproductive rate of Dendrobium huoshanense and survival rate of cultivation are raised. On one hand, production cycle of Dendrobium huoshanense can be shortened, and output and quality of Dendrobium huoshanense are raised. On the other hand, medicinal resource leakage of Dendrobium huoshanense is solved so as to obtain the optimum economical and ecological benefits.
Description
Technical field
The present invention relates to a kind of implantation methods of Dendrobidium huoshanness, further relate to a kind of method of utilizing micro-ecological technique to improve Dendrobidium huoshanness survival rate.
Technical background
Dendrobium is the second largest genus of the orchid family, nearly 1500 kinds of the whole world, there are 74 kinds of 2 mutation in China, wherein can hyoscine have a kind more than 40, be mainly distributed in south China and the southwest of China.Wherein Dendrobidium huoshanness is the endangered rareness species of China, be described as first of " the celestial grass of China ", it is the rare traditional Chinese medicine that Huoshan County is exclusive, have original producton location geographical sign, there is long history in China, there is high medicinal health value, have nourishing Yin and clearing heat, promote the production of body fluid beneficial stomach, moisten the lung and relieve the cough, improving eyesight keeps fit and anticancer, anti-ageing and strengthen the effect such as immunity, its active ingredient is mainly dendrobine and polysaccharide, is widely used at present drinks such as being processed into health products, medicine and stem of noble dendrobium soup, dendrobium wine.
Probiotics is according to microecology principle, for the regulating and controlling microbial ecological balance, utilizes useful micropopulation member or its promotion material to make.Since from last century, the Chu Mei Cheney husband of section advocates drinking acid milk and can have good health and a long life in Europe, the probiotics various places of being also from then on popular all over the world, and with surprising rapidity, good effect accepted by more crowds.
Because Dendrobidium huoshanness seed is tiny, sprout difficulty, seed germination rate is low; Add the living environment that it is special, cause its seedling percent low, the quantity of plant is few, therefore utilizes micro-ecologic planting Dendrobidium huoshanness to have broad prospects.
Summary of the invention
The invention provides a kind of implantation methods that improves Dendrobidium huoshanness survival rate, comprise preparation, the Dendrobidium huoshanness implantation methods of micro-ecological bacterial liquid.
The whole step of cultivation Dendrobidium huoshanness is:
(1) preparation of micro-ecological bacterial liquid
A, heterogenous fungus protein are 0.02-0.1 hundred million containing viable count in every gram of matrix;
B, white muscardine fungi stoste are 20-100 hundred million containing viable count in every gram of stoste;
C, photosynthetic bacteria stoste are 1-100 hundred million containing viable count in every gram of stoste;
D, biological organic fungi-manure (nutritive water), its compound method comprises the steps:
Get the former dew water distribution of 0.5-1.5kg EM, get 4-6kg brown sugar cold water and melt and add the former dew of EM, at 18-25 DEG C of bottom fermentation 24-30 hour; After this, add every gram to be the hay bacillus stoste 0.5-1kg of 10-100 hundred million containing viable count, and add the 40-70 degree brewing spirit of 2.5-5kg egg, 2.5-5kg milk powder, 2.5-5kg analysis for soybean powder, 2.5-5kg bone meal, 2.5-5kg fish meal, 2.5-5kg edible seaweed powder, 5-8kg, and 5-8kg rice vinegar, be that 8-10% left and right adds water by fermentation by state's liquid proportional; Accordingly, by weight ratio, described heterogenous fungus protein, white muscardine fungi stoste, photosynthetic bacteria stoste and the each portion of biological organic fungi-manure (nutritive water), the 100-1000 part that adds water as spray liquid for spraying; Add water 100-500 part as watering liquid for pouring; Add water 10-50 part as filling with root liquid for filling with root.
The micro-ecological bacterial liquid that adopts said method preparation, its using method is mainly to spray as main.The method spraying is determined as the case may be, conventionally after seedling replanting, Dendrobidium huoshanness plant is sprayed, and now joins existing spray.Under optimal way, within every 7 days, spray the spray liquid of 100 parts of once adding water, also according to circumstances spray the spray liquid of 1000 parts of once adding water every day.Especially need after the rain to add spray once, dilute the concentration reducing to make up because of rainwater.
In addition, according to weather condition, under in arid situation, can adopt above-mentioned join pouring liquid for irrigate, the concentration of irrigation liquid is walked according to fertile soil degree, damage by disease and insect degree.In addition, for because of the heavier plant of the harmful bacteria extent of damage, can utilize and fill with root liquid irrigating root, the concentration of irrigation liquid is also walked according to the extent of damage.
The invention has the advantages that, micro-ecological bacterial liquid provided by the invention is mainly made up of micro-ecological killing pests and suppressing bacteria agent, photosynthesis promoter, biological organic fungi-manure (nutritive water).What micro-ecological killing pests and suppressing bacteria agent adopted is the fungi that the mankind is had to health-care effect, the such as bacterial classification such as heterogenous fungus protein, white muscardine fungi, and a kill insects does not kill beneficial insect, makes harmful bacteria there is no living space.Utilize photosynthetic bacteria (Photosynthetic Bacteria, be called for short PSB) photosynthesis promoter can promote plant growth, strengthen crop disease-resistant diseases prevention ability, degrading pesticide residues and fixing poisonous and harmful heavy metallic, can promote again soil substance to transform, increase soil fertility, so Dendrobidium huoshanness is used its preparation, can receive the effect of increasing of quality and production.Biological organic fungi-manure also claims nutritive water, action principle has been simulated digestive system, organic nutrient substance is processed and produced the material that oligosaccharides, mycoprotein, glucose, enzyme etc. are easily absorbed by plants through fermenting enzyme through probios such as saccharomycete, Bacillus acidi lactici, hay bacillus, photosynthetic bacteria, actinomycetes, impose on root with 10-1000 multiple doubly, thereby directly absorbed as the nutriment of Dendrobidium huoshanness.Bacterium liquid of the present invention can be used as the medicament of sterilization and disinsection, can be used as again organic manure and uses, and only needs regularly to spray, and can improve the survival rate of Dendrobidium huoshanness seedling.
Embodiment
1, the preparation of micro-ecological bacterial liquid
Micro-ecological bacterial liquid of the present invention is mainly by heterogenous fungus protein, white muscardine fungi stoste, photosynthetic bacteria stoste, and biological organic fungi-manure (nutritive water) preparation obtains.
1) preparation of heterogenous fungus protein agricultural chemical
Taking natural pond liquid as basic culture solution, in 400 grams of natural pond liquid: the ratio preparation that water is 100 grams, with NaOH or HCL adjust pH, add sucrose or the glucose of 10 grams, the urea of 1.5 grams, pack the standard dress triangular flask of 100 milliliters of zymotic fluids into by 250 ml volumes containers, at 121 DEG C of high temperature with 0.106MPa, sterilizing 20 minutes under condition of high voltage, cooling rear access 3 ferfas are Curvularia lunata (the Curvulaia lunata of 5 days age, and/or Alternariaspp (Alternaria alternata CL), AA) bacterium piece, be 150rpm in liquid fermentation and culture condition, oscillation and fermentation cultivation 7 days on the constant-temperature table of 28 DEG C, after a large amount of hypha body to be formed, stop fermentation, be filtered dry zymotic fluid and obtain hypha body, pulverize hypha body for subsequent use as secondary seed bacterium,
By high-quality natural pond slag 10kg: scale grass 10kg, sucrose 400g, rice bran 600g, the dry matter weight ratio of urea 80g is mixed, then adds 9.5kg water and make water content be adjusted to 45%; Then regulating initial pH value is 7.0, under 121 DEG C, the HTHP of 0.106MPa, sterilizing 30 minutes, after cooling above-mentioned hypha body pulverizing body being done to secondary seed bacterium is seeded in the solid fermentation medium taking natural pond slag as main matrix, 26 DEG C of temperature, there are enough throughputs, continuous darkness is cultivated or illumination/dark hockets for each 12 hours, stir once every day, carry out large scale fermentation and cultivate 7 days, obtaining in every gram of matrix is the heterogenous fungus protein agricultural chemical of 0.02-0.1 hundred million containing CL and/or AA viable count.
2) preparation of biopesticide white muscardine fungi stoste
Get the 500g larva of a silkworm with batrytis (Chinese medicine pharmacy is on sale, conventionally price 0.1 yuan/gram) and make powder, get 5kg brown sugar cold water and melt and add larva of a silkworm with batrytis powder to mix, 18 DEG C of fermentations of normal temperature 24 hours, to produce a large amount of bubbles for well.
Get 5kg silkworm and leap up to pulverize with the thin analysis for soybean powder of 5kg and add in the bucket in fermentation, add water taking solid-liquid ratio as 8-10%, a bucket planted agent anti-fermentation overflow that has living space, builds fermentation with plastics.The liquid level of the white muscardine fungi fermenting has pollen shape white to form in steamed stuffed bun, total viable count 10,000,000,000/gram more than.White muscardine fungi in the larva of a silkworm with batrytis, through purifying, does not contain other probio in the white muscardine fungi liquid of preparation, does not affect insecticidal effect, can preserve about 6 months for subsequent use.Add the dilatation of again fermenting of the nutriments such as brown sugar, silkworm leap up, analysis for soybean powder.In the biopesticide white muscardine fungi stoste of preparing at said process, under optimum state, in every gram of stoste, be 20-100 hundred million containing viable count.
3) preparation of photosynthetic bacteria stoste
Prepare photosynthetic bacteria culture medium by following component:
At the photosynthetic bacteria seed culture fluid through accessing 1/10-1/5 in the photosynthetic bacteria culture medium of sterilizing, 15 DEG C-20 DEG C of temperature; Intensity of illumination is that under 8001ux-20001ux condition, anaerobism is cultivated 3-10 days, and obtaining in every gram of stoste is the photosynthetic bacteria stoste of 1-100 hundred million containing viable count.In described photosynthetic bacteria stoste, photosynthetic bacteria is that Rhodospirillum Rhodospirillium, Rhodopseudomonas Rhodopseudomonas, red bacterium belong to any strain bacterium or their any combination in Rhodobacter.
4), the preparation of biological organic fungi-manure (nutritive water)
Get the former dew of 1kg EM (market 1kg/20 on sale unit) water distribution, get 5kg brown sugar cold water and melt and add the former dew of EM, 18 DEG C of fermentations of normal temperature 24 hours, to produce a large amount of bubbles for well.After this, add every gram to be the hay bacillus stoste 0.5-1kg of 10-100 hundred million containing viable count, and get 2.5kg egg, 2.5kg milk powder, 2.5kg analysis for soybean powder, 2.5kg bone meal, 2.5kg fish meal, 2.5kg edible seaweed powder, 5kg rice vinegar, 5kg off-white wine, solid-liquid ratio is that 8-10% left and right adds water, and leaves space fermentation in bucket.The biological organic fungi-manure (nutritive water) fermenting has the fragrance of rice wine, total amount of probiotics 10,000,000,000/gram more than, can preserve about 6 months for subsequent use.
2, utilize the concrete grammar of above-mentioned micro-ecological bacterial liquid plantation Dendrobidium huoshanness seedling as follows:
Above-mentioned for planting the micro-ecological bacterial liquid of Dendrobidium huoshanness, can be used for configuring spray liquid, pouring liquid and fill with root liquid.By weight ratio, get heterogenous fungus protein, white muscardine fungi stoste, photosynthetic bacteria stoste and the each portion of biological organic fungi-manure (nutritive water), the 100-1000 part that adds water can be configured to spray liquid for spraying.Get heterogenous fungus protein, white muscardine fungi stoste, photosynthetic bacteria stoste and the each portion of biological organic fungi-manure (nutritive water), the 100-500 part that adds water can be configured to water liquid for pouring.Get heterogenous fungus protein, white muscardine fungi stoste, photosynthetic bacteria stoste and the each portion of biological organic fungi-manure (nutritive water), the 10-50 part that adds water can be configured to fill with root liquid for filling with root.
The micro-ecological bacterial liquid that adopts said method preparation, its using method is mainly to spray as main.The method root concrete condition spraying and walking sprays Dendrobidium huoshanness plant conventionally after seedling replanting, now joins existing spray.Under optimal way, within every 7 days, spray the spray liquid of 100 parts of once adding water, also according to circumstances spray the spray liquid of 1000 parts of once adding water every day.Especially need after the rain to add spray once, dilute the concentration reducing to make up because of rainwater.
In addition, according to weather condition, under in arid situation, can adopt above-mentioned join pouring liquid for irrigate, the concentration of irrigation liquid is walked according to fertile soil degree, damage by disease and insect degree.In addition, for because of the heavier plant of the harmful bacteria extent of damage, can utilize and fill with root liquid irrigating root, the concentration of irrigation liquid is also walked according to the extent of damage.
Claims (4)
1. an implantation methods that improves Dendrobidium huoshanness survival rate, is characterized in that, comprises following processing step:
A, heterogenous fungus protein are 0.02-0.1 hundred million containing viable count in every gram of matrix;
B, white muscardine fungi stoste are 20-100 hundred million containing viable count in every gram of stoste;
C, photosynthetic bacteria stoste are 1-100 hundred million containing viable count in every gram of stoste;
D, biological organic fungi-manure (nutritive water), its compound method comprises the steps:
Get the former dew water distribution of 0.5-1.5kg EM, get 4-6kg brown sugar cold water and melt and add the former dew of EM, at 18-25 DEG C of bottom fermentation 24-30 hour; After this, add every gram to be the hay bacillus stoste 0.5-1kg of 10-100 hundred million containing viable count, and add the 40-70 degree brewing spirit of 2.5-5kg egg, 2.5-5kg milk powder, 2.5-5kg analysis for soybean powder, 2.5-5kg bone meal, 2.5-5kg fish meal, 2.5-5kg edible seaweed powder, 5-8kg, and 5-8kg rice vinegar, be that 8-10% left and right adds water by fermentation by state's liquid proportional; Accordingly, by weight ratio, described heterogenous fungus protein, white muscardine fungi stoste, photosynthetic bacteria stoste and the each portion of biological organic fungi-manure (nutritive water), the 100-1000 part that adds water as spray liquid for spraying; Add water 100-500 part as watering liquid for pouring; Add water 10-50 part as filling with root liquid for filling with root.
2. the cultivation method of micro-ecologic planting Dendrobidium huoshanness as claimed in claim 1, is characterized in that, regularly utilizes spray liquid to spray.
3. the cultivation method of micro-ecologic planting Dendrobidium huoshanness as claimed in claim 1, is characterized in that, within every 7 days, sprays the spray liquid of 100 parts of once adding water.
4. the cultivation method of micro-ecologic planting Dendrobidium huoshanness as claimed in claim 1, is characterized in that, also comprises: utilize pouring liquid to irrigate, and utilize the step of filling with root liquid irrigating root.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355691A (en) * | 2014-10-14 | 2015-02-18 | 广西容县顺昌隆生物科技有限公司 | Organic fertilizer applied to dendrobium officinale as well as application of organic fertilizer |
| CN104365457A (en) * | 2014-09-19 | 2015-02-25 | 安徽省霍山县五峰山石斛开发有限公司 | Large dendrobium huoshanense seedling planting method |
| CN106396767A (en) * | 2016-08-30 | 2017-02-15 | 柳州市泓吉农业科技有限公司 | Leaf surface spraying fertilizer for dendrobium officinale |
-
2013
- 2013-02-28 CN CN201310061663.XA patent/CN104012263A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365457A (en) * | 2014-09-19 | 2015-02-25 | 安徽省霍山县五峰山石斛开发有限公司 | Large dendrobium huoshanense seedling planting method |
| CN104355691A (en) * | 2014-10-14 | 2015-02-18 | 广西容县顺昌隆生物科技有限公司 | Organic fertilizer applied to dendrobium officinale as well as application of organic fertilizer |
| CN106396767A (en) * | 2016-08-30 | 2017-02-15 | 柳州市泓吉农业科技有限公司 | Leaf surface spraying fertilizer for dendrobium officinale |
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Application publication date: 20140903 |