KR102553815B1 - Cosmetic composition for preventing skin aging using poly-l-lactic acid - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles using poly-l-lactic acid (PLLA) and, more specifically, to a cosmetic composition containing: 100 parts by weight of an active ingredient; and 0.1 to 10 parts by weight of a polymersome containing PLLA, wherein the active ingredient contains sodium hyaluronate, collagen, and hydrolyzed elastin. The cosmetic composition for preventing skin aging consisting of the above ingredient is applicable to an MTS method and not only has an excellent skin aging prevention effect due to improved skin penetration effect but also has low skin toxicity not to cause skin trouble.

Description

Cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) {COSMETIC COMPOSITION FOR PREVENTING SKIN AGING USING POLY-L-LACTIC ACID}

The present invention relates to a cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA), and more specifically, it is applicable to the MTS method and exhibits an excellent skin aging prevention effect due to the improvement of skin penetration effect, It relates to a cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA), which has low toxicity and does not cause skin trouble.

After conducting a 12-year follow-up on 954 people, a research team led by Professor Dan Belsky of Duke University found that aging begins at the age of 26 and accelerates when the body reaches 38 years of age. The research team studied adults living in New Zealand who were born between 1972 and 1973. To measure the inside of the body, kidney, liver, lung, metabolism and immune functions, cholesterol levels, cardiopulmonary function, and dental conditions were used as indicators. In addition, telomere length, which determines aging by regulating cell division at the end of chromosomes, and the state of capillaries in the back of the eyes, were measured by a total of 18 items, including body age. As aging begins, skin regenerative cells also decrease by the age of 25. In addition to aging, modern people's skin, which is exposed to various external harmful environments and stresses, is exposed to various damages. As the average lifespan increases, there is a need to develop cosmetics to improve these signs of aging. Based on the development of biochemistry and molecular biology, a small amount of signal substances such as growth factors that exist in our body have been discovered, and based on this, the aging theory of the living body is being reestablished. In addition, this signal substance decreases in vivo as age increases, which reveals that the decrease in collagen, elastin, and hyaluronic acid is closely related to our aging. As these three components decrease with age, skin aging phenomena such as reduced skin elasticity, skin wrinkles, and pigmentation appear. Various cosmetics are being developed to improve phenomena such as destruction of collagen, decrease in collagen synthesis, loss of elastin, and melanin pigmentation due to deterioration of skin physiology, which are phenomena appearing in the skin epidermis and dermis.

The skin, which is the largest organ of the human body, is composed of several different layers with an area of 1.8 m2, a thickness of 2 to 4 mm, and a weight of 3 kg. Each layer has a specialized function, and the main layers are the epidermis and dermis ( dermis), etc. The epidermal layer is the outermost layer of the skin and is divided into five layers: the stratum corneum, the clear layer, the granular layer, the spinous layer, and the basal layer. There is a moisture barrier layer between the transparent layer and the granular layer in the epidermal layer, which is a special membrane that has passed chemical properties and serves to prevent the penetration of foreign substances from the outside and the escape of moisture from the inside of the skin. Due to this moisture-blocking film, not only foreign substances but also water cannot penetrate into the skin, which makes it difficult for the active ingredients of cosmetics to penetrate deep into the skin. The dermis layer occupies more than 90% of the skin tissue, so it is called the dermis because it is called the real skin that determines the skin shape. The reticular layer under the papillary layer is a hard and irregular net-shaped connective tissue that occupies most of the dermal layer, and is composed of collagen, elastin, and hyaluronic acid.

On the other hand, MTS (Microneedle Therapy System), a physical percutaneous absorption method, is a fine needle for which the safety and effectiveness of the human body has been established, and it has been proven to be considered an effective method for promoting absorption of cosmetic formulations. It is a device to increase the absorption rate of active ingredients by more than 80% by physically creating a very fine planned passage of 0.25mm to 0.5mm. No matter how low-molecular cosmetics are, when simply applied, the absorption rate is difficult to exceed 10%, so physically create fine gaps to increase the absorption rate. Even if safety has been proven with household needles, fine needles can cause wounds.

Based on the development of biochemistry and molecular biology, a small amount of signal substances such as growth factors that exist in our body have been discovered, and based on this, the aging theory of the living body is being reestablished. In addition, this signal substance decreases in vivo as age increases, and it is revealed that the decrease in collagen, hyaluronic acid, and elastin is closely related to our aging. Collagen is a type of protein made up of amino acids. It serves as a scaffold for cell activities by binding cells or tissues like glue, and plays a role in connecting all cells by existing between cells in the body. Elastin is a spring-shaped hard protein, present between collagen and entangled in marriage tissue. Since it has a spring structure, when the elastin content is reduced, the structure becomes loose, which makes the skin more likely to sag and cause fine wrinkles. Hyaluronic acid is a type of acidic mucopolysaccharide widely present in connective tissues of animals such as eye elements, chicken crests, and joint fluid. am. Compared to other mucopolysaccharides, it has a high molecular weight and very high water absorption. There is a report that 1 g of hyaluronic acid occupies a volume of about 1 L in water. Before skin aging progresses, the physiological activity of skin cells is active. In fibroblasts in the dermis, the synthesis and metabolism of collagen are vigorous, and the synthesis of GAGS, a type of glycoprotein including hyaluronic acid and elastin, is also active, giving elasticity, softness, flexibility and moisture to the skin. However, modern people's skin is exposed to various external harmful environments and stresses, resulting in various damages. Various skin aging phenomena such as calmness, age spots, and dullness appear. In order to solve these skin problems, a cosmetic composition containing the corresponding raw materials may be required.

In addition, liposomes are currently widely used not only in pharmaceutical fields but also in cosmetics and foods, which have the advantage of being able to stably deliver physiologically active ingredients. It is used for injections such as intravenous and spinal injections, and is also used as an ointment for wounds. This is to promote skin regeneration by allowing effective ingredients in the ointment to penetrate into the dermis rather than the epidermis, which is the outer surface of the skin, and this property is also being used in the cosmetic field. The usefulness of liposomes in cosmetics is to encapsulate water-soluble active ingredients with low absorption and affinity for the hydrophobic skin in liposomes made of phospholipids, thereby improving skin absorption problems while passing through the water barrier of the epidermal layer. It has the ability to do it. Liposomes are largely classified into multilamellar vesicles (MLV) and Unilamella vesicles (ULV), and ULVs can be divided into large unilamella vesicles and small unilamella vesicles. MLV has a size of 500-5,500 nm (nanometers) and the amount that can be encapsulated in liposomes is 5-15%, and LUV is 200-800 nm in size and the amount that can be encapsulated in liposomes is 36-65% , and the size of the SUV is 20 to 150 nm, and the amount that can be encapsulated in the liposome is 0.5 to 1.0%. LUV has the highest amount of bioactive substances, but it is unstable, and the structure of human skin has a multilamella structure, so MLV has affinity with the skin, but it is too large to penetrate the skin. Therefore, it is considered that the liposome particles used in cosmetics are higher in SUV form because they must have stability and effectiveness in intradermal delivery. In addition, the penetration rate can be increased by splitting the molecular weight of polymers larger than the skin layer, such as collagen, elastin, and hyaluronic acid, through the liposome method. The particle size is at least a hydrophilic material and a phospholipid similar to that of the skin to deliver active ingredients deep into the skin.

Korean Patent Registration No. 10-0564108 (2006.03.20.) Korean Patent Registration No. 10-2224032 (2021.03.02.)

An object of the present invention is to use polyelastic acid (PLLA), which is applicable to the MTS method and exhibits an excellent skin aging prevention effect due to improved skin penetration, as well as an effect that does not cause skin trouble due to low skin toxicity. It is to provide a cosmetic composition for improving skin wrinkles.

The object of the present invention consists of 100 parts by weight of an active ingredient and 0.1 to 10 parts by weight of a polymersome containing polyelastic acid (PLLA), and the active ingredient contains sodium hyaluronate, collagen and hydrolyzed elastin. It is achieved by providing a cosmetic composition for preventing skin aging using polyelastic acid (PLLA), characterized in that it becomes.

According to a preferred feature of the present invention, the active ingredient is composed of glycerin, purified water, caprylic/capric triglyceride, hydrogenated lecithin, cholesterol, sodium hyaluronate, collagen, and hydrolyzed elastin. .

According to a more preferred feature of the present invention, the active ingredient is glycerin 100 parts by weight, purified water 13 to 17 parts by weight, caprylic / capric triglyceride 5 to 7 parts by weight, hydrogenated lecithin 2 to 3 parts by weight, 0.5 to 0.7 parts by weight of cholesterol, 0.1 to 0.15 parts by weight of sodium hyaluronate, 0.1 to 0.15 parts by weight of collagen, and 0.1 to 0.15 parts by weight of hydrolyzed elastin.

According to a more preferred feature of the present invention, the sodium hyaluronate, collagen and hydrolyzed elastin are emulsified to 120 to 200 nanometers.

According to a more preferred feature of the present invention, the polyelastic acid (PLLA)-containing polymersome is obtained by adding palmitoyl tripeptide to a mixture of polyelastic acid (PLLA) and polyethylene glycol in a ratio of 5: 2. to be manufactured.

According to a more preferred feature of the present invention, the active ingredient further contains 0.1 to 5 parts by weight of perilla leaf extract relative to 100 parts by weight of glycerin contained in the active ingredient.

According to a more preferred feature of the present invention, the perilla leaf extract is prepared by mixing and extracting 200 to 1000 parts by weight of lower alcohol having 1 to 4 carbon atoms in 100 parts by weight of perilla leaves.

According to a more preferred feature of the present invention, the cosmetic composition for skin wrinkle improvement using the polyelastic acid (PLLA) is one selected from the group consisting of lotion, eye cream, moisture cream, cleansing product, serum and ampoule It is to be applied as a formulation.

The cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) according to the present invention can be applied to the MTS method and exhibits excellent skin aging prevention effects due to improved skin penetration effect, as well as low skin toxicity, so as to prevent skin troubles. It shows an excellent effect of providing a cosmetic composition exhibiting an effect that does not induce.

Hereinafter, a preferred embodiment of the present invention and the physical properties of each component will be described in detail, but this is to be explained in detail so that a person having ordinary knowledge in the art to which the present invention belongs can easily practice the invention, This is not meant to limit the technical spirit and scope of the present invention.

The cosmetic composition for preventing skin aging using polyelastic acid (PLLA) according to the present invention is made by mixing a polymersome containing polyelastic acid (PLLA) with an active ingredient, 100 parts by weight of active ingredient and polyellactic acid It is preferable to consist of 0.1 to 10 parts by weight of polymersome containing acid (PLLA).

The active ingredient contains sodium hyaluronate, collagen, and hydrolyzed elastin, and the active ingredient containing the above ingredients exhibits excellent anti-aging and anti-wrinkle effects.

In addition, the sodium hyaluronate, collagen, and hydrolyzed elastin supplement the sodium hyaluronate, collagen, and hydrolyzed elastin components that decrease in the dermal layer, which decreases with age, to prevent skin aging or wrinkles. role in giving

In addition, it is preferable to liposome through a liposome method so that the sodium hyaluronate, collagen, and hydrolyzed elastin can penetrate deeply into the skin. At this time, as described above, polyelastic acid (PLLA) containing Preferably, liposomes are mixed with polymersomes.

In addition, the above sodium hyaluronate, collagen and hydrolyzed elastin were stirred with a high-pressure homogenizer in a state heated to a temperature of 75 to 80 ° C, cooled to 40 ° C, and microemulsified device (Microfluidizer, MFD) It is preferable to emulsify after making the average particle exhibit 120 to 200 nanometers by using.

As described above, when treated 7 to 10 times using a microemulsion machine, the average particle shows about 120 to 150 nanometers, and when treated 10 to 20 times using a microemulsion machine, 150 to 200 nanometers Indicates the average particle size.

Sodium hyaluronate, collagen, and hydrolyzed elastin exhibiting the average particle size as described above significantly improve the skin penetration effect.

At this time, the active ingredient is preferably composed of glycerin, purified water, caprylic / capric triglyceride, hydrogenated lecithin, cholesterol, sodium hyaluronate, collagen and hydrolyzed elastin, 100 parts by weight of glycerin , 13 to 17 parts by weight of purified water, 5 to 7 parts by weight of caprylic/capric triglyceride, 2 to 3 parts by weight of hydrogenated lecithin, 0.5 to 0.7 parts by weight of cholesterol, 0.1 to 0.15 parts by weight of sodium hyaluronate , It is more preferably composed of 0.1 to 0.15 parts by weight of collagen and 0.1 to 0.15 parts by weight of hydrolyzed elastin.

When the content of sodium hyaluronate is less than 0.1 part by weight relative to 100 parts by weight of the glycerin, the anti-aging effect is reduced, and when the content of sodium hyaluronate exceeds 0.15 part by weight, for preventing skin aging produced through the present invention The viscosity of the cosmetic composition increases excessively, making it difficult to apply to the MTS method.

In addition, the active ingredient further contains 0.1 to 5 parts by weight of perilla leaf extract compared to 100 parts by weight of glycerin contained in the active ingredient, which serves to improve skin elasticity and improve skin wrinkles.

The perilla leaf extract is prepared by mixing and extracting 200 to 1000 parts by weight of lower alcohol having 1 to 4 carbon atoms with 100 parts by weight of perilla leaf. It contains at least one compound selected from the group consisting of glucuronide, apigenin-diglucuronide, luteolin-glucuronide, and rosmarinate, and exhibits excellent skin elasticity improvement and skin wrinkle improvement effects.

If the content of the perilla leaf extract is less than 0.1 parts by weight, the above effect is insignificant, and if the content of the perilla leaf extract exceeds 5 parts by weight, the above effect is not greatly improved, and the formulation stability of the cosmetic composition may be reduced. not desirable

The polyelastic acid (PLLA)-containing polymersome contains 0.1 to 10 parts by weight, and is prepared by adding palmitoyl tripeptide to a mixture of polyelastic acid (PLLA) and polyethylene glycol at a ratio of 5: 2. , Lipid-like biocompatibility and self-association of biodegradable polymers do not adsorb to the molecular double-layered vesiclo peptide and the thin film of the polymersome, thereby fundamentally blocking the denaturation of the active ingredient as well as effective substances such as internal peptides. It shows the advantage that can keep the activity intact.

The polymersome has a particle diameter of 300 to 1000 nanometers and is synthesized in a size that can be absorbed into the skin. The polymersome composed of ingredients not only enhances the skin penetration effect of the active ingredient, but also contains the palmitoyl tripeptide component in the polymersome itself, so that the skin aging prevention effect can be further improved.

If the content of the polymersome is less than 0.1 part by weight, the above effect is insignificant, and if the content of the polymersome exceeds 10 parts by weight, the content of the active ingredient is relatively excessively reduced, thereby reducing the effect of preventing skin aging, as well as cosmetic The viscosity of the composition is too strong, so it may be difficult to apply it to the MTS method.

The formulation of the anti-aging cosmetic composition comprising the above components is not particularly limited, but considering the frequency of use or the part applied to the skin for the anti-skin aging effect, lotion, eye cream, moisturizing cream, cleansing product, serum And it is preferably applied in one formulation selected from the group consisting of ampoules.

Hereinafter, a method for preparing a cosmetic composition for preventing skin aging using polyelastic acid (PLLA) according to the present invention and physical properties of the cosmetic composition prepared by the manufacturing method will be described with examples.

<Production Example 1>

Sodium hyaluronate, collagen, and hydrolyzed elastin were each heated to a temperature of 78 ° C, stirred with a high-pressure homogenizer, cooled to 40 ° C, and averaged by using a microfluidizer (MFD) was made to represent 120 to 200 nanometers.

<Preparation Example 2> Preparation of active ingredient

80.2g of glycerin, 12mL of purified water, 5g of caprylic/capric triglyceride, 2g of hydrogenated lecithin, 0.5g of cholesterol, 0.1g of sodium hyaluronate prepared in Preparation Example 1, 0.1g of collagen and hydrol An active ingredient was prepared by mixing 0.1 g of lyzed elastin.

<Preparation Example 3> Preparation of polymersomes containing polyelastic acid (PLLA)

After mixing and dissolving polyelastic acid (PLLA) and polyethylene glycol in a 5:2 weight ratio in chloroform, 1% by weight of palmitoyl tripeptide-5 based on the total weight of the reactants was added, and then the polymer was dissolved through microfluidics technology. After synthesizing the bit, chloroform was removed and cured to prepare a polymer bit containing polyelastic acid (PLLA).

<Preparation Example 4> Preparation of perilla leaf extract

800 parts by weight of ethanol having a mass concentration of 50% was mixed with 100 parts by weight of perilla leaves, put in a microwave oven, and heated at 90W for 5 minutes to prepare a perilla leaf extract.

<Example 1>

5 parts by weight of the polymersome containing the polyelastic acid (PLLA) prepared in Preparation Example 3 was mixed with 100 parts by weight of the active ingredient prepared in Preparation Example 2, and stirred at a speed of 150 rpm for 10 minutes to polyelac. A cosmetic composition for improving skin wrinkles using tic acid (PLLA) was prepared.

<Example 2>

Proceed in the same manner as in Example 1, but by mixing 0.1 part by weight of the polyellactic acid (PLLA)-containing polymersome prepared in Preparation Example 3 to improve skin wrinkles using polyelastic acid (PLLA). was manufactured.

<Example 3>

Proceed in the same manner as in Example 1, but mix 10 parts by weight of the polymersome containing polyelastic acid (PLLA) prepared in Preparation Example 3, and use a cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA). was manufactured.

<Example 4>

Proceed in the same manner as in Example 1, but further mix 3 parts by weight of the perilla leaf extract prepared in Preparation Example 4 with respect to 100 parts by weight of glycerin contained in the active ingredient prepared in Preparation Example 2 to obtain a cosmetic composition for preventing skin aging manufactured.

<Comparative Example 1>

Glycerin 80.2g, purified water 12mL, caprylic/capric triglyceride 5g, hydrogenated lecithin 2g, cholesterol 0.5g, sodium hyaluronate 0.1g, collagen 0.1g and hydrolyzed elastin 0.1g were mixed A cosmetic composition for preventing skin aging was prepared.

The physical properties of the cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) prepared in Example 1 were measured and are shown in Table 1 below.

<Table 1>

In addition, the skin toxicity of the cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) prepared in Example 1 of the present invention was measured and shown in Table 3 below.

[However, for skin toxicity, the ISO 10993-5 method was used, and a survival rate of 70% or more was determined to have no cytotoxicity.

* Cell line: Vero Cell (African Green Monkey, KCLB No - 10081)

* Reagents: Dubelcco’s Modified Eagle’s Medium (DMEM, Sigma), Kanamycin sulfate (SAMCHUN), Sodium bicarbonate (DUKSAN), Antibiotic-Antimytic (GenDEPOT), Fetal bovine serum (GenDEPOT)

* Solvent: DMEM medium + 10% FBS

* Test conditions: After contacting the cells and the sample eluate for 24 hours, the absorbance is measured at 570 nm by treating with Thiazolyl Blue Tetrazolium Bromide reagent.

* Control group: negative control group-Phosphate Buffer Saline, positive control group-Dimethyl sulfoxide, solvent control group-solvent

* Result notation method: Survival rate (%) was calculated using the following formula.

Survival rate (%) = {(Sample treated group OD570 / Sample untreated group OD570) / (Sample untreated group OD570 - Sample untreated group OD650)} × 100]

<Table 2>

<Table 3>

As shown in Table 1, it can be seen that the cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) prepared in Example 1 of the present invention has low skin toxicity.

In addition, the anti-wrinkle effect of the cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) prepared in Examples 1 to 4 and Comparative Example 1 was measured and shown in Table 4 below.

{However, for the wrinkle improvement effect, apply the cosmetic composition prepared for 50 women in their 30s to 50s with wrinkles around the eyes for 4 weeks, 2g per day for 4 weeks, and then measure the degree of improvement using a 3-point scale method was measured and expressed as an average value.

3 points: very improved, 2 points: slightly improved, 3 points: no improvement}

<Table 4>

As shown in Table 4, it can be seen that the cosmetic composition prepared through Examples 1 to 4 of the present invention exhibits an excellent anti-wrinkle effect compared to Comparative Example 1.

Therefore, the cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) according to the present invention can be applied to the MTS method and exhibits excellent skin aging prevention effects due to improved skin penetration effect, as well as low skin toxicity. It shows an effect that does not cause trouble.

Claims (8)

It consists of 0.1 to 10 parts by weight of polymersomes containing 100 parts by weight of active ingredients and polyelastic acid (PLLA),
The active ingredient contains sodium hyaluronate, collagen and hydrolyzed elastin,
A cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA), characterized in that the sodium hyaluronate, collagen and hydrolyzed elastin are emulsified to 120 to 200 nanometers.
The method of claim 1,
The active ingredient is polyelastic acid (PLLA), characterized in that it consists of glycerin, purified water, caprylic / capric triglyceride, hydrogenated lecithin, cholesterol, sodium hyaluronate, collagen and hydrolyzed elastin ) Cosmetic composition for improving skin wrinkles using.
The method of claim 2,
The active ingredient is glycerin 100 parts by weight, purified water 13 to 17 parts by weight, caprylic / capric triglyceride 5 to 7 parts by weight, hydrogenated lecithin 2 to 3 parts by weight, cholesterol 0.5 to 0.7 parts by weight, sodium high A cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA), comprising 0.1 to 0.15 parts by weight of alluronate, 0.1 to 0.15 parts by weight of collagen, and 0.1 to 0.15 parts by weight of hydrolyzed elastin.
delete The method of claim 1,
The polyelastic acid (PLLA)-containing polymersome is prepared by adding palmitoyl tripeptide to a mixture of polyelastic acid (PLLA) and polyethylene glycol in a ratio of 5: 2. A cosmetic composition for improving skin wrinkles using (PLLA).
The method of claim 1,
A cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA), characterized in that the active ingredient further contains 0.1 to 5 parts by weight of perilla leaf extract compared to 100 parts by weight of glycerin contained in the active ingredient.
The method of claim 6,
The perilla leaf extract is prepared by mixing and extracting 200 to 1000 parts by weight of lower alcohol having 1 to 4 carbon atoms in 100 parts by weight of perilla leaves. A cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA).
The cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA) according to any one of claims 1 to 3 and 5 to 7 is a group consisting of lotion, eye cream, moisture cream, cleansing product, serum and ampoule A cosmetic composition for improving skin wrinkles using polyelastic acid (PLLA), characterized in that it is applied as one formulation selected from