KR101895037B1 - Hydrogel comprising spicule and use thereof - Google Patents

Abstract

The present invention relates to a hydrogel composition comprising a speckle, a hairdressing sheet comprising the hydrogel composition, and a cosmetic patch.

Description

HYDROGEL COMPRISING SPICULE AND USE THEREOF < RTI ID = 0.0 >

The present invention relates to a hydrogel composition comprising a speckle, a hairdressing sheet comprising the hydrogel composition, and a cosmetic patch.

Skin is the primary barrier of the human body. It protects the internal organs of the body from external stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. This important skin also becomes aged gradually as the age of the human body grows, and as a result, skin elasticity loss, keratinization, wrinkle formation, skin atrophy and the like appear. This aging of the skin not only causes wrinkles on the skin surface but also changes the entire line of the face.

Such aging of the skin can slow down a little by providing nourishment to the skin when the skin becomes rough or sunburned and tired, and by applying elasticity to the facial skin. In order to supply nutrients to damaged skin, it is necessary to apply natural ingredients such as Oatana citrus, massage cream, nutritional packs, cleansing packs, bleach packs, etc. and apply them on the face with beauty tissue or other auxiliary means It is generally used.

However, these materials have limited use due to safety problems such as irritation, erythema, and redness when applied to the skin, or the effect is insignificant, so that the effect of skin function improvement or wound healing can not be expected substantially. Accordingly, there is a desperate need to develop a new composition which is safer and more effective in living body than existing skin external composition compositions.

With regard to the technique of delivering the active ingredient of the cosmetic composition to the skin, US Patent No. 5,322,683 discloses a method of making anhydrous formulation so as not to contain water, but such a method is problematic in that the formulation is limited to powder, It is difficult to apply it to a water-soluble active ingredient. On the other hand, there is a hydrogel patch of European patent EP 0840597B1 and the like in the prior art related to a skin application patch type for delivering an effective ingredient, but these techniques also have a disadvantage that the delivery of an effective ingredient is insufficient.

In recent years, development and consumption of a mask sheet have been actively carried out in order to effectively increase the skin absorption of contents by utilizing various types of sheets. Furthermore, not only general sheet types but also hydrogels, bio-celluloses and the like are being widened. Among them, the application fields of hydrogels are various. Most of the hydrogels have a function of controlling the release of the drug contained in the hydrogel gradually toward the skin while maintaining the gel state. Many of the hydrogel mask sheets developed in the past are effective when a large amount of the active ingredient is formulated The components were separated, the viscoelasticity was poor, and the adhesion of the skin was poor.

Accordingly, development of a cosmetic composition, a beauty sheet, and a patch which is effectively absorbed in the skin to provide a skin effect improving effect with less irritation is desired.

European Patent No. 0840597

It is an object of the present invention to provide a hydrogel composition comprising a speckle.

It is another object of the present invention to provide a hairdressing sheet comprising the hydrogel composition.

It is still another object of the present invention to provide a cosmetic patch for local skin care comprising the hydrogel composition.

According to an aspect of the present invention, there is provided a hydrogel composition comprising a speckle.

Specifically, the spicule may include 0.001 wt% to 10 wt% of the total weight of the hydrogel composition. There is an advantage of exhibiting good skin regeneration, water and water absorption promoting effect within the above-mentioned range, and stabilizing the formulation of the composition.

In the present invention, " speckle " is also called sponge, and sponge animal is a primitive marine creature with no muscles, nerves, or organs. More than 5,000 species are present, It is a living thing. The skeleton is a tissue that functions as a skeleton that has a needle shape present in the body of invertebrate animals, and is mainly composed of silicic acid and calcium carbonate. The fibrous skeleton of sponges has absorption ability according to the capillary phenomenon and is applied to various fields such as medical use. For example, the fragments of sponges contain antibiotics for treating osteomyelitis and are used for the treatment and prevention of diseases.

In the present invention, the term "hydrogel " refers to a gel comprising water as a dispersion medium, wherein the hydrosol loses its fluidity due to cooling, or the hydrophilic polymer having a three-dimensional network structure and a microcrystalline structure contains water, Or the like. The hydrogel of the electrolytic polymer is highly absorbent and is widely used as an absorbent polymer in various fields. In the hydrogel, the expansion ratio may change discontinuously due to the phase transition by temperature, pH, and the like.

In addition, the hydrogel may exhibit temperature sensitivity.

In the present invention, the term " thermosensitive hydrogel " refers to a hydrogel in which the sol moves to the gel or the phase transition occurs to the sol according to the temperature, and gelation gelation in the present invention means that the gelation has viscoelasticity and the polymer forms a three-dimensional net structure as the temperature increases and becomes a state in which the polymer is not dissolved in the solvent and remains in the solvent. Particularly, it exists in the form of a sol at room temperature. When it is applied to the surface of skin and becomes higher in temperature, it can be gelled. Therefore, the thermosensitive hydrogel of the present invention can be applied directly to the skin surface, or can be attached using a mask sheet or a beauty patch, and can sufficiently exhibit its effect by increasing stagnation and adhesion time of the active ingredient through gelation according to temperature Can be done.

Specifically, the hydrogel may include a branched gel polymer, an electrolytic gel polymer, a skin affinity enhancer, a natural biomaterial, a polyhydric alcohol, a functional additive, and water.

More specifically, the branched gelatinized polymer may be at least one polymer selected from the group consisting of galactomannan, clumoconan, guar gum, locust bean gum, carob bean gum, and pluronic, and the electrolytic gelling polymer Carrageenan, xanthan and gellan. ≪ Desc / Clms Page number 12 > In one embodiment of the present invention, a hydrogel composition including a carob bean gum and carrageenan as a polymer together with spicule was prepared.

More specifically, the hydrogel composition may further comprise a cosmetic active ingredient.

In the present invention, the " active ingredient " refers to a cosmetic or nutritional ingredient capable of exhibiting an effective effect on the skin, and examples thereof include retinol, retinyl palmitate, retinyl acetate, retinoic acid, coenzyme Q10, elastin, collagen, hyaluronic acid, , Collagen, caffeine, chitosan, ascorbic acid, ascorbyl glucoside, alpha bissabolol, tocopherol, tocopherol acetate, arbutin, niacinamide, adenosine, retinol acetate, vitamins A, D and E and natural extracts , But is not limited thereto.

In addition, the natural extract may be selected from the group consisting of aloe, green tea, ginseng, red ginseng, pearl, vinegar, pine leaf, ginkgo leaf, propolis, mulberry leaf, silkworm, snail slime, kakaduplum, camucam, yashiyama, squalane, caviar, broccoli, Berry, locust, hyaluronic acid, acerola, chlorella, mangosteen, guava, corn, carrot, caffeine, hammamelis, spirulina, salmon roe, giant kelp, packing, But are not limited to, those extracted from one or more selected from the group consisting of mulberry, edelweiss, chamomile, lavender, peppermint, eucalyptus, lemon balm, oregano, tea tree, golden,

In addition, it was confirmed that the hydrogel composition containing the speckle showed an increase in the effect of accelerating the absorption of water in the case of the present invention (Table 4), and it was also found that the skin permeation of the active ingredient was also applied in the form of an essence, (Table 6), it was confirmed that the active ingredient was contained in the hydrogel composition containing spicule and the skin permeability was significantly increased when it was applied.

Accordingly, the hydrogel composition containing the speckle of the present invention, and the hydrogel composition further containing the active ingredient may be one for promoting skin regeneration, promoting the absorption of water, or promoting absorption of the active ingredient.

In the present invention, " skin regeneration " means a restoration process of skin tissue against damage caused by external or internal causes of the skin. Damage due to external causes may include ultraviolet rays, external contaminants, wound, trauma, etc. The damage caused by the internal causes may be stress.

In one example of the present invention, it was confirmed that when the human skin fibroblast culture was treated with spicule, the cell number increase effect was remarkably increased as compared with the control group not treated with the spicule (Table 2) Lt; RTI ID = 0.0 > hydrogel < / RTI > containing water can promote the skin regeneration effect by promoting an increase in the number of skin cells.

In addition, the hydrogel composition containing the speckle of the present invention can exhibit the skin regeneration effect as well as the skin elasticity enhancement and wrinkle reducing effect, which are effects that can be derived therefrom.

In the present invention, "skin elasticity" is expressed by elastic fibers composed of elastin existing in the dermal layer. Such elastic fibers have a very low elastic modulus such as rubber and are easily deformed by a small force, When the force is removed, it easily returns to the original shape. In addition, the elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin. Elastine is a kind of elastin, which is found only in elastic fibers such as desmosine and isodesmosine derived from lysine. It is a protein composed of one amino acid. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In the present invention, "skin elasticity enhancement" means that elastic fibers composed of elastin are present together with collagen fibers called collagen, and elastic elasticity is maintained or increased in a state where elastin and collagen are sufficiently present.

The term "skin wrinkle" in the present invention means a skin caused by a decline in skin, which may be caused by a cause of a gene, a decrease in collagen and elastin present in the skin dermis, and an external environment.

In the present invention, "improvement of skin wrinkles" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

In the present invention, the term " water content " refers to oil and / or moisture required by the skin, and it is possible to maintain the skin tissue by transferring the water content to the skin.

Spirulk penetrates into the basal layer of the skin to activate the therapeutic immune system of the human body, thereby helping regenerate the cells. In addition, it can reach the dermal layer when penetrating into the pores, thereby helping to transmit moisture and active ingredients deep into the skin .

In the present invention, it was confirmed that when the human skin fibroblast culture was treated with spicule, the cell number increase effect was remarkably enhanced as compared with the control group not treated with the spicule (Table 2), and the hydrogel composition containing the spicule (Table 4). As shown in Fig. In addition, when the active ingredient is applied to the skin in an essence form or in a hydrogel composition not containing spicule, the active ingredient is contained in the hydrogel composition containing spicule, and when applied, It was confirmed that the transmittance was significantly increased (Table 6).

It was confirmed from this that the hydrogel composition containing the speckle of the present invention was applied or attached as a cosmetic sheet, thereby promoting skin regeneration and promoting delivery of water or active ingredient.

Another aspect of the present invention provides a cosmetic sheet comprising the hydrogel composition comprising the speckle. Specifically, the cosmetic sheet may be one for promoting skin regeneration, promoting the absorption of water or absorbing active ingredients. The active ingredients that can be delivered and contained in the hydrogel composition of the present invention are as described above.

Examples of the material that can be used as the support of the sheet include rayon, urethane, latex, acrylic, etc., but not limited thereto, and can be suitably modified and used by using woven fabric or knitted fabric or materials capable of minimizing exposure to the outside have.

Yet another aspect of the present invention provides a cosmetic patch for topical skin care comprising a hydrogel composition comprising the speckle.

The term " topical skin " in the present invention refers to a skin of a specific part of a tissue, such as an eye, a mouth, a wrinkle, a clown, a ball, a chin, a hand, an elbow or a chest, Can be suitably manufactured in a size and shape necessary for managing the local skin as described above.

The term " skin care " refers to an action for effecting skin regeneration, skin texture improvement, skin elasticity enhancement, or skin wrinkle improvement, including, but not limited to, .

In the present invention, the " beauty sheet " or " beauty patch " can be utilized in various ways in use. The skin is impregnated with a liquid containing the active ingredient (a component for moisturizing, anti-allergy, antibacterial, whitening, wrinkle improvement, etc.) The hydrogel composition can be gelled in response to skin temperature to penetrate the skin.

The hydrogel composition containing the speckle of the present invention has excellent skin regeneration effect by itself. In addition, the composition of the present invention is excellent in the effect of transferring oil and moisture into the skin, and exhibits an effect of accelerating absorption of the active ingredient contained in the composition. Can be increased.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Manufacturing example  One. Spicule  Included Hydrogel  Produce

Hydrogels containing spicules were prepared by mixing the ingredients listed in Table 1 below. Specifically, the ingredients of phase 1 were mixed and dissolved in a dispenser tank at 1500 to 1700 rpm, homogenous 2000 to 2500 rpm, paddle 30 to 45 rpm and temperature 40 to 45 ° C, and mixed thoroughly at 80 ° C.

The components of Phase 2 were mixed for 15 to 20 minutes at 1500 to 1700 rpm of disper, 2000 to 2500 rpm of homogenous, 30 to 45 rpm of paddle and 80 to 85 캜 of temperature, dispersed at 800 to 1200 rpm for 10 minutes, Lt; / RTI > The mixture was then thoroughly mixed and cooled to 60-65 占 폚.

The components of phase 3 are then mixed for 20 minutes at 1500 rpm to 1700 rpm, homogenous 2000 to 2500 rpm, paddle 30 to 45 rpm and temperature 60 to 65 ° C, and the resulting mixture is introduced into the mixture of phase 1 and phase 2 at 60 to 65 ° C Mix thoroughly. Thereafter, the paddle was degassed at 15 to 22 rpm.

Prize Ingredients Example 1 Example 2 Example 3 Top 1 Purified water to 100 to 100 to 100 Disodium iodide 0.02 0.02 0.02 Phase 2 glycerin 0.1 30 70 Carp glue sword 0.01 3 10 Hydrolyzed sponges (spicules) 0.01 4 10 Carrageenan 5 2 0.01 Phase 3 PIGGY-60 Hydrogenated Castor Oil 0.1 3 5 Caprylic / capric triglyceride 0.1 3 10 antiseptic 0.1 2 4 incense 0.005 0.01 0.3 Adenosine 0.001 One 30 Sum 100 wt% 100 wt% 100 wt%

A hydrogel sheet for skin cosmetic was prepared by inserting a rayon mesh fabric into a core of a high-temperature hydrogel composition prepared as described above. In addition, a hydrogel composition in a fluid state at a temperature of 45 to 95 캜 was molded into a beauty sheet or patch and cooled to room temperature to prepare a gel-state hydrogel.

Comparative Example  One. Spicule  Not included Hydrogel  Preparation of composition

The components listed in Table 2 below were mixed to prepare a hydrogel composition not containing spicule. The specific mixing method was the same as that described in Example 1 above.

Prize Ingredients content( weight% ) Top 1 Purified water to 100 Disodium iodide 0.02 Phase 2 glycerin 30 Carp glue sword 3 Carrageenan 3 Phase 3 PIGGY-60 Hydrogenated Castor Oil 0.2 Caprylic / capric triglyceride 0.1 antiseptic 3 incense 0.1 Adenosine One Sum 100

Experimental Example  One. Spiculous  Check skin rejuvenation effect

CCK (Cell counting kit) -8 assay was used to confirm skin regeneration effect through skin cell proliferation. Human skin fibroblasts were cultured in DMEM (Dulbecco's Modification of Eagle's Medium) medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin at 5% CO ₂ at 37 ° C. 1,000 human dermal fibroblasts were plated on a 96-well plate, and the cells were treated with the spleen, followed by confirming the degree of cell proliferation after 3 days. For the measurement, CCK-8 solution was added to the cell culture solution (100 μl / well), incubated for 3 hours in an incubator, absorbance was measured at 450 nm, and cell number increase was examined based on the standard curve. The results are shown in Table 3 below.

sample Cell number after 3 days Control group 1735 Spicule treated with 0.01 ug / ul 4256 Spuuk 4ug / ul treatment 4874 Treatment with 10 μg / μl sp. 5263

As shown in Table 3, when the spicule was treated and cultured for 3 days, the cell number increase effect was remarkably enhanced as compared with the control group not treated with spicule. Therefore, it has been confirmed that when the composition containing the speucule of the present invention is applied or adhered to the skin, it can exhibit excellent skin regeneration effect by promoting the proliferation of the skin cells. In addition, as the concentration of treated speckle was increased, the cell number increase effect was further increased.

Experimental Example  2. Watery  Skin transfer rate measurement

Experiments were conducted to confirm the skin absorption promoting effect of the hydrolyzed composition containing the speckle prepared in Comparative Example 1 and Examples 1 to 3 above.

Specifically, the hydrogel compositions of Comparative Example 1 and Examples 1 to 3 were applied, and after 30 minutes, the skin moisture was measured using a scalar moisture checker for skin MY-707S.

sample Skin moisture (%) Comparative Example 1 45 Example 1 62 Example 2 69 Example 3 75

As shown in Table 4, it was found that the skin moisture of Examples 1 to 3 was significantly higher than that of Comparative Example 1 which did not contain the speckle. Also, as the concentration of the contained speckle increased, the moisture of the skin also increased Respectively.

Experimental Example  3. On hydrogel  Measure skin permeability of active ingredients included

Experiments were conducted to confirm the skin absorption promoting effect of the active ingredients of the hydrogel compositions containing the spirals of Examples 1 to 3 prepared above. The hydrogel compositions of Comparative Example 1 and Examples 1 to 3 containing adenosine, which is the same effective ingredient, were applied and the skin permeability was measured (Table 5).

No. sample One Its essence 2 Comparative Example 1 3 Example 1 4 Example 2 5 Example 3

Specifically, the dorsal skin of 8 to 10-week-old hairless mice was removed and the subcutaneous fat and tissues were removed with physiological saline, and then the skin was well spread and used for the experiment. After the stratum corneum was put in a donor compartment of Franz diffusion cell of the hairless hamster skin, it was sandwiched between receptor compartments, and then only the essence product was applied to the surface of the skin, The hydrogel compositions of Examples 1 to 3 were applied. In the recipient compartment, pH 7.4 phosphate buffered saline was added and stirring was continued at 300 rpm while maintaining the temperature at 37 ° C. At this time, the area of the skin contacting the recipient compartment was 2.0 cm 2, and the capacity of the recipient compartment was 5 ml. After 1.0 ml of each sample was administered to the skin surface, the sample was collected using a fraction collector.

All samples were filtered through a 13-mm disk filter (pore size 0.45 μm) before column injection and analyzed with a Waters 2690 alliance separation module (Waters, USA) with a photodiode array detector system 996 (Waters, USA) . Xterra RP18 (5 μ m, 4.6 x 205 mm, Waters, USA) column was used as a stationary phase. The mobile phase was methanol, water and acetic acid buffer in a mixed solvent of 10:80:10, flow rate of 0.8 ml / min Was detected at a wavelength of 280 nm. Specifically, the skin permeability of adenosine, an active ingredient contained in its essence and hydrogel composition, was measured.

The amount of adenosine accumulated per unit skin area was plotted against time, and the adenosine flux in the equilibrium state was calculated from the slope of the region showing linearity. The Lag time (T _L ) was determined from the time the straight line passed through the x-axis. Adenosine flow and permeation coefficient in the equilibrium state were calculated and compared using the following Equation (1).

[Formula 1]

J _s is the adenosine flow in equilibrium, S is the area of permeation, (dQ / dt) _ss is the slope obtained by tabulating the amount of adenosine passing through the skin in equilibrium over time, C _d is the saturation concentration, K _p represents the transmission coefficient.

The skin permeability measured according to the above analysis method is as shown in Table 6 below.

Sample No. Delay time (hr) Adenosine flow
(ug / cm ² / hr) Percutaneous Transmittance (%) One 5.3 6.20 2.50 2 3.3 65.33 10.54 3 2.3 78.86 15.22 4 2.1 113.23 29.17 5 1.7 128.06 33.16

As shown in the results of Table 6, when the hydrogel compositions of Comparative Example 1 and Examples 1 to 3 were applied as compared to the case of applying the essence, the delivery effect of adenosine and the skin permeation promoting effect . In addition, in Examples 1 to 3 containing spicule, it was confirmed that the delivery effect of adenosine, which is an effective ingredient, and the skin permeation promoting effect were more excellent than Comparative Example 1 in which spicule was not included. From the results, it can be confirmed that the hydrogel composition including the speckle of the present invention shows a very excellent effect on the skin to be contained in the active ingredient contained therein, and that the application of various active ingredients is very useful as a cosmetic product.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (13)

Carrageenan as a speckle, a branched gelling polymer as a carboplatin gum, and an electrolytic gelling polymer,
Wherein the speckle is contained in an amount of 0.01 to 10% by weight based on the total weight of the total composition. delete delete delete The hydrogel composition of claim 1, wherein the hydrogel composition further comprises a cosmetic active ingredient. 6. The composition of claim 5, wherein the active ingredient is selected from the group consisting of retinol, retinyl palmitate, retinyl acetate, retinoic acid, coenzyme Q10, elastin, collagen, hyaluronic acid, ceramide, collagen, caffeine, chitosan, ascorbic acid, ascorbyl glucoside, Wherein the hydrogel composition is at least one selected from the group consisting of alphabinol, tocopherol, tocopherol acetate, arbutin, niacinamide, adenosine, retinol acetate, vitamins A, D, E and natural extracts. [7] The method of claim 6, wherein the natural extract is selected from the group consisting of aloe, green tea, ginseng, red ginseng, pearl, wood vinegar, pine needle, ginkgo leaf, propolis, mulberry leaf, silkworm, snail slime, kakaduplum, camucam, yashiyama, squalane, , Broccoli, blueberry, location hazel, acerola, chlorella, mangosteen, guava, corn oil, carrot, caffeine, hammamelis, spirulina, salmon roe, guttae, giant kelp, packing, Wherein the hydrogel composition is extracted from at least one selected from the group consisting of red raspberries, red raspberries, mint, edelweiss, chamomile, lavender, peppermint, eucalyptus, lemon balm, oregano, tea tree, gold, 6. The method according to claim 1 or 5,
Wherein said hydrogel composition is for regenerating skin, promoting absorption of water, or promoting absorption of an active ingredient. A hairdressing composition comprising the hydrogel composition of claim 1. The beauty sheet according to claim 9, wherein the beauty sheet is for regenerating skin, promoting absorption of water, or promoting absorption of an active ingredient. 11. The composition of claim 10, wherein the active ingredient is selected from the group consisting of retinol, retinyl palmitate, retinyl acetate, retinoic acid, coenzyme Q10, elastin, collagen, hyaluronic acid, ceramide, collagen, caffeine, chitosan, ascorbic acid, ascorbyl glucoside, Wherein the at least one cosmetic composition is at least one selected from the group consisting of alpha-aminobenzene, alphabinol, tocopherol, tocopherol acetate, arbutin, niacinamide, adenosine, retinol acetate, vitamins A, D and E and natural extracts. 12. The method according to claim 11, wherein the natural extract is selected from the group consisting of aloe, green tea, ginseng, red ginseng, pearl, wood vinegar, pine needles, ginkgo leaf, propolis, mulberry leaf, silkworm, snail slime, kakaduplum, camucam, yashiyama, squalane, , Broccoli, blueberry, location hazel, acerola, chlorella, mangosteen, guava, corn oil, carrot, caffeine, hammamelis, spirulina, salmon roe, guttae, giant kelp, packing, Wherein the hair is extracted from at least one member selected from the group consisting of red raspberries, red raspberries, mint, edelweiss, chamomile, lavender, peppermint, eucalyptus, lemon balm, oregano, tea tree, gold, A cosmetic patch for topical skin care comprising the hydrogel composition of claim 1.