DK3472325T3 - Fremgangsmåde til målrettet DNA-ændring i planteceller - Google Patents
Fremgangsmåde til målrettet DNA-ændring i planteceller Download PDFInfo
- Publication number
- DK3472325T3 DK3472325T3 DK17739336.0T DK17739336T DK3472325T3 DK 3472325 T3 DK3472325 T3 DK 3472325T3 DK 17739336 T DK17739336 T DK 17739336T DK 3472325 T3 DK3472325 T3 DK 3472325T3
- Authority
- DK
- Denmark
- Prior art keywords
- plant cells
- targeted dna
- dna modification
- targeted
- modification
- Prior art date
Links
- 230000008836 DNA modification Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2016998 | 2016-06-20 | ||
| PCT/NL2017/050408 WO2017222370A1 (en) | 2016-06-20 | 2017-06-20 | Method for targeted dna alteration in plant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3472325T3 true DK3472325T3 (da) | 2024-05-21 |
Family
ID=57104119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17739336.0T DK3472325T3 (da) | 2016-06-20 | 2017-06-20 | Fremgangsmåde til målrettet DNA-ændring i planteceller |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US11371051B2 (da) |
| EP (1) | EP3472325B1 (da) |
| JP (1) | JP7160465B2 (da) |
| KR (1) | KR102555713B1 (da) |
| AU (1) | AU2017282623B2 (da) |
| CA (1) | CA3027869A1 (da) |
| DK (1) | DK3472325T3 (da) |
| ES (1) | ES2981548T3 (da) |
| FI (1) | FI3472325T3 (da) |
| IL (1) | IL263595B2 (da) |
| WO (1) | WO2017222370A1 (da) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3365356B1 (en) | 2015-10-23 | 2023-06-28 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| EP3557981A1 (en) * | 2016-12-22 | 2019-10-30 | Keygene N.V. | Method for targeted alteration of duplex dna |
| WO2018115389A1 (en) * | 2016-12-22 | 2018-06-28 | Keygene N.V. | Methods of targeted genetic alteration in plant cells |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| KR20190117536A (ko) * | 2017-02-15 | 2019-10-16 | 키진 엔.브이. | 식물 세포에서 표적 유전적 변형 방법 |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| JP7191388B2 (ja) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子 |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| EP3676376A2 (en) | 2017-08-30 | 2020-07-08 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| AU2018352592A1 (en) | 2017-10-16 | 2020-06-04 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| CA3088052A1 (en) | 2018-01-11 | 2019-07-18 | KWS SAAT SE & Co. KGaA | Optimized plant crispr/cpf1 systems |
| WO2020004652A1 (ja) * | 2018-06-29 | 2020-01-02 | 学校法人同志社 | エムリカサンを含む製剤 |
| WO2020191243A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| CA3177481A1 (en) | 2020-05-08 | 2021-11-11 | David R. Liu | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| KR102496764B1 (ko) * | 2020-09-25 | 2023-02-07 | 대한민국 | 토마토 화분 발달 관련 유전자 교정용 가이드 rna 및 이의 용도 |
| WO2022219181A1 (en) | 2021-04-15 | 2022-10-20 | Keygene N.V. | Co-regeneration recalcitrant plants |
| KR102675540B1 (ko) * | 2021-07-21 | 2024-06-13 | 경상국립대학교산학협력단 | 유전자 교정을 이용한 병 저항성이 조절된 토마토 식물체의 제조방법 및 상기 제조방법에 의해 제조된 토마토 식물체 |
| WO2024133851A1 (en) | 2022-12-22 | 2024-06-27 | Keygene N.V. | Regeneration by protoplast callus grafting |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2562261T3 (da) * | 2007-12-21 | 2015-11-30 | Keygene Nv | Forbedret mutagenesefremgangsmåde under anvendelse af polyethylenglycolmedieret indføring af mutagene nukleobaser i planteprotoplaster |
| UA118090C2 (uk) * | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
| WO2014065596A1 (en) * | 2012-10-23 | 2014-05-01 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| US20150067922A1 (en) * | 2013-05-30 | 2015-03-05 | The Penn State Research Foundation | Gene targeting and genetic modification of plants via rna-guided genome editing |
| ES2896755T3 (es) | 2013-07-11 | 2022-02-25 | Modernatx Inc | Composiciones que comprenden polinucleótidos sintéticos que codifican proteínas relacionadas con CRISPR y ARNsg sintéticos y procedimientos de uso |
-
2017
- 2017-06-20 DK DK17739336.0T patent/DK3472325T3/da active
- 2017-06-20 IL IL263595A patent/IL263595B2/en unknown
- 2017-06-20 CA CA3027869A patent/CA3027869A1/en active Pending
- 2017-06-20 JP JP2018565404A patent/JP7160465B2/ja active Active
- 2017-06-20 ES ES17739336T patent/ES2981548T3/es active Active
- 2017-06-20 EP EP17739336.0A patent/EP3472325B1/en active Active
- 2017-06-20 US US16/310,764 patent/US11371051B2/en active Active
- 2017-06-20 FI FIEP17739336.0T patent/FI3472325T3/fi active
- 2017-06-20 AU AU2017282623A patent/AU2017282623B2/en active Active
- 2017-06-20 WO PCT/NL2017/050408 patent/WO2017222370A1/en unknown
- 2017-06-20 KR KR1020197000966A patent/KR102555713B1/ko active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ES2981548T3 (es) | 2024-10-09 |
| IL263595B1 (en) | 2023-07-01 |
| CA3027869A1 (en) | 2017-12-28 |
| IL263595B2 (en) | 2023-11-01 |
| KR102555713B1 (ko) | 2023-07-14 |
| WO2017222370A1 (en) | 2017-12-28 |
| IL263595A (en) | 2019-02-28 |
| AU2017282623A1 (en) | 2019-01-03 |
| EP3472325A1 (en) | 2019-04-24 |
| KR20190020035A (ko) | 2019-02-27 |
| US11371051B2 (en) | 2022-06-28 |
| JP2019517805A (ja) | 2019-06-27 |
| JP7160465B2 (ja) | 2022-10-25 |
| EP3472325B1 (en) | 2024-04-03 |
| AU2017282623B2 (en) | 2023-09-21 |
| FI3472325T3 (fi) | 2024-06-18 |
| US20190367933A1 (en) | 2019-12-05 |
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