DK3456846T3 - Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre - Google Patents

Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre Download PDF

Info

Publication number
DK3456846T3
DK3456846T3 DK18201501.6T DK18201501T DK3456846T3 DK 3456846 T3 DK3456846 T3 DK 3456846T3 DK 18201501 T DK18201501 T DK 18201501T DK 3456846 T3 DK3456846 T3 DK 3456846T3
Authority
DK
Denmark
Prior art keywords
systems
methods
nucleic acid
barcode marking
marking nucleic
Prior art date
Application number
DK18201501.6T
Other languages
English (en)
Inventor
David A Weitz
Allon Moshe Klein
Ilke Akartuna
Marc W Kirschner
Linas Mazutis
Original Assignee
Harvard College
Univ Vilnius
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Univ Vilnius filed Critical Harvard College
Application granted granted Critical
Publication of DK3456846T3 publication Critical patent/DK3456846T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DK18201501.6T 2014-04-21 2015-04-17 Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre DK3456846T3 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201461982001P 2014-04-21 2014-04-21
US201462065348P 2014-10-17 2014-10-17
US201462066188P 2014-10-20 2014-10-20
US201462072944P 2014-10-30 2014-10-30
EP15783629.7A EP3134536B1 (en) 2014-04-21 2015-04-17 Systems and methods for barcoding nucleic acids

Publications (1)

Publication Number Publication Date
DK3456846T3 true DK3456846T3 (da) 2022-07-11

Family

ID=54333034

Family Applications (3)

Application Number Title Priority Date Filing Date
DK18201501.6T DK3456846T3 (da) 2014-04-21 2015-04-17 Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre
DK15783629.7T DK3134536T3 (da) 2014-04-21 2015-04-17 Systemer og fremgangsmåder til at stregkodemarkere nukleinsyrer
DK17198030.3T DK3299469T3 (da) 2014-04-21 2015-04-17 Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre

Family Applications After (2)

Application Number Title Priority Date Filing Date
DK15783629.7T DK3134536T3 (da) 2014-04-21 2015-04-17 Systemer og fremgangsmåder til at stregkodemarkere nukleinsyrer
DK17198030.3T DK3299469T3 (da) 2014-04-21 2015-04-17 Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre

Country Status (9)

Country Link
EP (3) EP3134536B1 (da)
JP (1) JP6853667B2 (da)
CN (1) CN107075543B (da)
AU (1) AU2015250034B2 (da)
CA (1) CA2946144A1 (da)
DK (3) DK3456846T3 (da)
HK (1) HK1253013A1 (da)
LT (3) LT3456846T (da)
WO (1) WO2015164212A1 (da)

Families Citing this family (141)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
US9597644B2 (en) * 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4397767A3 (en) 2012-08-14 2024-07-31 10X Genomics, Inc. Microcapsule compositions and methods
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2931919B1 (en) 2012-12-14 2019-02-20 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP2016511243A (ja) 2013-02-08 2016-04-14 テンエックス・ジェノミクス・インコーポレイテッド ポリヌクレオチドバーコード生成
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP4219010A1 (en) 2014-04-10 2023-08-02 10X Genomics, Inc. Methods for encapsulating and partitioning reagents
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
EP3161157B1 (en) * 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digital pcr barcoding
KR102531677B1 (ko) 2014-06-26 2023-05-10 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
JP2017532024A (ja) * 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド コンポジット単一細胞核酸分析のための液滴ベースの方法および機器
JP2017532042A (ja) 2014-10-29 2017-11-02 10エックス ゲノミクス,インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 10エックス ジェノミクス, インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
CN116064731A (zh) 2015-03-13 2023-05-05 哈佛学院院长及董事 使用扩增测定细胞
AU2016248995B2 (en) 2015-04-17 2022-04-28 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
SG11201803646YA (en) 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
WO2017083375A1 (en) * 2015-11-10 2017-05-18 Illumina, Inc. Inertial droplet generation and particle encapsulation
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
PT3882357T (pt) 2015-12-04 2022-09-05 10X Genomics Inc Métodos e composições para análise de ácidos nucleicos
US20180363050A1 (en) * 2015-12-07 2018-12-20 Raindance Technologies, Inc. Multiplexing in partitions using primer particles
EP3263715B1 (en) 2016-06-28 2020-01-08 Hifibio Method for transcriptome analysis of single cells
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
WO2017192633A1 (en) 2016-05-02 2017-11-09 Procure Life Sciences Inc. Macromolecule analysis employing nucleic acid encoding
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017218486A1 (en) * 2016-06-14 2017-12-21 Mission Bio, Inc. Methods and compositions for emulsification of solid supports in deformable beads
EP3260201A1 (en) 2016-06-21 2017-12-27 Hifibio Microfluidic filtration unit and related microfluidic system
CN110114473A (zh) * 2016-11-23 2019-08-09 斯特拉斯堡大学 靶分子的串联条形码添加以便以单实体分辨率对靶分子进行绝对定量
CN110462053A (zh) * 2016-12-21 2019-11-15 加利福尼亚大学董事会 使用基于水凝胶的液滴进行单细胞基因组测序
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) * 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3585364B1 (en) * 2017-02-24 2021-04-28 The Regents of the University of California Particle-drop structures and methods for making and using the same
CN110431233B (zh) 2017-03-13 2022-08-12 吉加根公司 用于单细胞的大量平行组合分析的系统和方法
LT3375889T (lt) 2017-03-17 2020-06-25 Hifibio Sas Vienos ląstelės analizė
JP6846832B2 (ja) 2017-05-02 2021-03-24 国立大学法人 東京大学 一細胞の非破壊的計測情報とゲノム関連情報とを一体的に検出する方法
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CA3064205A1 (en) 2017-05-26 2018-11-29 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US20210246488A1 (en) * 2017-08-14 2021-08-12 Mission Bio, Inc. Method and compositions for evaluating emulsion uniformity
US10821442B2 (en) 2017-08-22 2020-11-03 10X Genomics, Inc. Devices, systems, and kits for forming droplets
MA50079A (fr) 2017-09-07 2020-07-15 Juno Therapeutics Inc Procédés d'identification de caractéristiques cellulaires relatives à des réponses associées à une thérapie cellulaire
WO2019060685A1 (en) * 2017-09-22 2019-03-28 Bioventures, Llc SYSTEM AND METHOD FOR PHENOTYPE ANALYSIS AND POLYNUCLEOTIDE SEQUENCING OF BIOLOGICAL PARTICLES THROUGH A DETERMINISTIC BARCODE
CN111372574A (zh) * 2017-09-29 2020-07-03 加利福尼亚大学董事会 单分散乳液的制备方法
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
KR20200080252A (ko) * 2017-10-09 2020-07-06 소마젠 인크 단일 분자 서열분석 및 핵산서열 특성화를 위한 고유한 분자 식별자
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20220362771A1 (en) * 2018-07-13 2022-11-17 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
EP3821033A1 (en) * 2018-07-13 2021-05-19 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
CN112189139B (zh) * 2018-09-26 2024-10-29 北卡罗来纳-查佩尔山大学 用于改进测定的化合物、组合物和方法
WO2020069298A1 (en) 2018-09-28 2020-04-02 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets
US20220010373A1 (en) * 2018-11-07 2022-01-13 The University Of Tokyo Method for detecting genome-related information of cell coexisting with at least one type of test substance
US11512356B2 (en) * 2018-11-08 2022-11-29 Tokitae Llc Systems and methods for particle multiplexing in droplets
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
JP2022511931A (ja) 2018-12-13 2022-02-01 ディーエヌエー スクリプト 細胞および生体分子上へのオリゴヌクレオチドの直接合成
CN109851711B (zh) * 2018-12-29 2020-09-25 苏州绘真生物科技有限公司 可溶性水凝胶微球及其制备方法与在单细胞检测中的应用
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
EP3924505A1 (en) 2019-02-12 2021-12-22 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
WO2020186094A1 (en) * 2019-03-12 2020-09-17 Universal Sequencing Technology Methods for single cell intracellular capture and its applications
WO2020206285A1 (en) * 2019-04-05 2020-10-08 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
CA3138367A1 (en) 2019-04-30 2020-11-05 Encodia, Inc. Methods for preparing analytes and related kits
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
WO2021046475A1 (en) * 2019-09-06 2021-03-11 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
GB2604481A (en) 2019-10-10 2022-09-07 1859 Inc Methods and systems for microfluidic screening
CN111172257A (zh) * 2020-01-16 2020-05-19 南方科技大学 一种带编码的凝胶微粒及其制备方法和应用
WO2021147069A1 (zh) * 2020-01-23 2021-07-29 青岛华大智造普惠科技有限公司 基于液滴微流控的单细胞测序及应用
WO2021194699A1 (en) * 2020-03-24 2021-09-30 Cellecta, Inc. Single cell genetic analysis
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022223561A1 (en) * 2021-04-20 2022-10-27 Simsen Diagnostics Ab Compositions and methods for cell-free nucleic acid isolation
CN113215233A (zh) * 2021-05-15 2021-08-06 墨卓生物科技(浙江)有限公司 一种用于单细胞测序的具有特定寡核苷酸序列的微球
CN117794646A (zh) 2021-06-04 2024-03-29 伊努梅里斯公司 用于单细胞条形码化和测序的组合物、方法和系统
WO2022256612A1 (en) * 2021-06-04 2022-12-08 Enumerix, Inc. Compositions, methods, and systems for single cell barcoding and sequencing
CN113584598A (zh) * 2021-06-17 2021-11-02 南方科技大学 一种单细胞文库的构建方法
WO2023025784A1 (en) * 2021-08-24 2023-03-02 Ecole Polytechnique Federale De Lausanne (Epfl) Optimised set of oligonucleotides for bulk rna barcoding and sequencing
CN113889192B (zh) * 2021-09-29 2024-02-27 西安热工研究院有限公司 一种基于深层降噪自编码器的单细胞RNA-seq数据聚类方法
EP4437128A1 (en) * 2021-12-01 2024-10-02 Vilnius University Methods for processing and barcoding nucleic acids
CN114177960B (zh) * 2021-12-15 2023-04-28 天津大学 基于超高频体声波谐振器的微液滴操纵方法以及装置
WO2023139272A1 (en) * 2022-01-24 2023-07-27 Scipio Bioscience Gelation device with piston
WO2023237180A1 (en) * 2022-06-07 2023-12-14 Alithea Genomics Sa Optimised set of oligonucleotides for bulk rna barcoding and sequencing
WO2024011226A1 (en) * 2022-07-07 2024-01-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cell-barcode recorder devices and methods
EP4331724A1 (en) * 2022-08-29 2024-03-06 Institut Curie Method of extracting a bead from a droplet
EP4435114A1 (en) 2023-03-24 2024-09-25 Miltenyi Biotec B.V. & Co. KG Syntheis of highly multiplexed oligo-barcoded fluorescent beads for single cell seq applications

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP2266687A3 (en) 2003-04-10 2011-06-29 The President and Fellows of Harvard College Formation and control of fluidic species
WO2005021151A1 (en) 2003-08-27 2005-03-10 President And Fellows Of Harvard College Electronic control of fluidic species
EP1861194A2 (en) 2005-03-04 2007-12-05 The President and Fellows of Harvard College Method and apparatus for forming multiple emulsions
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
US20070195127A1 (en) 2006-01-27 2007-08-23 President And Fellows Of Harvard College Fluidic droplet coalescence
JP2010520749A (ja) * 2007-02-27 2010-06-17 ノースウェスタン ユニバーシティ ナノ粒子への分子の結合
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20090181375A1 (en) * 2008-01-11 2009-07-16 Peter Brian J Method for detection of nucleic acid barcodes
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3587594B1 (en) * 2008-12-19 2022-04-13 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US8691509B2 (en) * 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
WO2010151776A2 (en) 2009-06-26 2010-12-29 President And Fellows Of Harvard College Fluid injection
EP3461558B1 (en) 2009-10-27 2021-03-17 President and Fellows of Harvard College Droplet creation techniques
US8586301B2 (en) * 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
WO2012048341A1 (en) * 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
DK2652155T3 (da) * 2010-12-16 2017-02-13 Gigagen Inc Fremgangsmåder til massiv parallelanalyse af nukleinsyrer i enkeltceller
EP3736281A1 (en) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
CN103703146A (zh) * 2011-07-19 2014-04-02 株式会社日立高新技术 核酸分析方法和核酸分析装置
WO2013116698A2 (en) * 2012-02-02 2013-08-08 Invenra, Inc. High throughput screen for biologically active polypeptides
EP2823064B1 (en) * 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
CN104619862B (zh) * 2012-06-15 2017-10-31 得克萨斯系统大学董事会 单细胞的多转录物的高通量测序
WO2014047561A1 (en) * 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
EP4094834A1 (en) * 2013-12-30 2022-11-30 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
EP3161157B1 (en) * 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digital pcr barcoding

Also Published As

Publication number Publication date
EP3134536A4 (en) 2017-12-20
EP3456846B1 (en) 2022-06-22
EP3134536A1 (en) 2017-03-01
JP6853667B2 (ja) 2021-03-31
JP2017515469A (ja) 2017-06-15
AU2015250034A1 (en) 2016-11-03
LT3134536T (lt) 2020-05-11
CA2946144A1 (en) 2015-10-29
DK3299469T3 (da) 2020-03-30
AU2015250034B2 (en) 2019-06-20
LT3299469T (lt) 2020-04-27
EP3299469B1 (en) 2020-03-04
WO2015164212A9 (en) 2016-10-13
EP3134536B1 (en) 2019-12-18
LT3456846T (lt) 2022-09-12
EP3299469A1 (en) 2018-03-28
WO2015164212A1 (en) 2015-10-29
HK1253013A1 (zh) 2019-06-06
EP3456846A1 (en) 2019-03-20
CN107075543A (zh) 2017-08-18
DK3134536T3 (da) 2020-02-03
CN107075543B (zh) 2021-11-16

Similar Documents

Publication Publication Date Title
DK3456846T3 (da) Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre
IL288424B (en) System and method for reading fluorescent markings in X-rays
IL259736A (en) Methods and systems for nucleic acid analysis
HK1250182A1 (zh) 核酸分析系統和方法
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3122870T3 (da) Fremgangsmåder og genetiske systemer til celleteknik
EP3194627C0 (en) METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACID SEQUENCING DATA
DK3744410T3 (da) System og fremgangsmåde til legetøjsgenkendelse
DK3386550T3 (da) Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer
DK3332034T3 (da) Systemer og fremgangsmåder til genomisk analyse
DK3108009T3 (da) Fremgangsmåder og sammensætninger til DNA-profilering
DK3129912T3 (da) Fremgangsmåde og system til sikring af data
SG11201610707RA (en) Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification
DK3690056T3 (da) Fremgangsmåder og produkter til fremstilling og indgivelse af nukleinsyre
DK3260813T3 (da) System og fremgangsmåde til afstandsbedømmelse
DK3175588T3 (da) Systemer og fremgangsmåder til beskedredigering
DK3505638T3 (da) Mikrofluid indretning, system og fremgangsmåde til studie af organismer
DK3152316T3 (da) Prøveklargøring til nukleinsyreamplifikation
DK3094731T3 (da) Saccharidmodificerede nukleinsyremolekyler
DK3359671T3 (da) Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation
DK3167076T3 (da) Fremgangsmåder og produkter til kvantificering af RNA-transkriptvarianter
DK3231131T3 (da) System og fremgangsmåde til at erstatte fælles identificerende data
DK3318001T3 (da) Fremgangsmåder og indretninger til håndtering af hash-tree-baserede datasignaturer
DK3172708T3 (da) Billetudstedelsesfremgangsmåde og -system
DK3231016T3 (da) Fotovoltaikmodul og fotovoltaiksystem