CN115429927A - Photo-thermal antibacterial dressing prepared from sodium alginate and apple polyphenol as well as preparation method and application of photo-thermal antibacterial dressing - Google Patents
Photo-thermal antibacterial dressing prepared from sodium alginate and apple polyphenol as well as preparation method and application of photo-thermal antibacterial dressing Download PDFInfo
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- CN115429927A CN115429927A CN202211117628.0A CN202211117628A CN115429927A CN 115429927 A CN115429927 A CN 115429927A CN 202211117628 A CN202211117628 A CN 202211117628A CN 115429927 A CN115429927 A CN 115429927A
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- dressing
- sodium alginate
- apple polyphenol
- antibacterial
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
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- A—HUMAN NECESSITIES
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Abstract
The invention belongs to the technical field of wound repair materials, and particularly relates to a sodium alginate-apple polyphenol compounded photo-thermal antibacterial dressing, and a preparation method and application thereof. The components of the antibacterial dressing have a synergistic effect, and under the irradiation of near infrared light, the antibacterial dressing has a good photo-thermal circulation effect, can improve the temperature of a wound and obviously enhance the sterilization effect; moreover, the loaded apple polyphenol has an antibacterial effect, and can be combined with a photothermal effect to synergistically improve the antibacterial effect, so that wound infection and inflammation are avoided on the premise of not adding antibiotics. In addition, the sodium alginate and the apple polyphenol have good effects of promoting wound healing, have good biocompatibility and high biological safety, and are very suitable for repairing various wounds.
Description
Technical Field
The invention belongs to the technical field of wound repair materials. More particularly, relates to a sodium alginate compounded apple polyphenol photothermal antibacterial dressing, a preparation method and an application thereof.
Background
Wounds and chronic diseases can cause wounds of different degrees, if the wounds do not heal for a long time, the normal life of a patient can be seriously influenced, and great harm is brought to health. Among them, if bacterial infection occurs to the wound, serious inflammation of the wound and even death of the wound can be caused. Therefore, the antibacterial healing of the wound is very important.
Medical dressings are a type of dressing used to cover skin wounds, and provide protection to the wound to promote healing. Many artificially synthesized and natural-based wound dressings are developed for wound repair, but the biosafety verification of the artificially synthesized dressings costs a lot and is time-consuming, while natural materials such as collagen, fibroin, sodium alginate, chitosan, inorganic minerals and the like are safe and non-toxic, have better biocompatibility, biodegradability, tissue repair property, antibacterial activity and hemostatic efficacy, and are more suitable for preparing wound dressings. In addition, another problem faced by most of the currently commercialized wound dressings is that most of the dressings do not have antibacterial performance, and in order to improve the antibacterial effect of the dressings, the chinese patent application CN111518288A discloses a composite hydrogel wound dressing which is prepared by taking aminated collagen and oxidized sodium alginate as base materials and loading two polypeptide antibiotics, namely polymyxin B sulfate and bacitracin, and has good wound healing promoting and antibacterial effects. Simply increasing the antimicrobial effect of the dressing by the addition of antibiotics can be potentially bio-toxic and can easily lead to the production of resistant bacteria and even superbacteria.
Disclosure of Invention
The invention aims to solve the technical problems that the existing natural source dressing has poor antibacterial property and is easy to cause the defects and the defects of drug-resistant bacteria or super bacteria by combining antibiotics, and provides the sodium alginate composite apple polyphenol photothermal antibacterial dressing with better antibacterial effect.
The invention aims to provide a preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing.
The invention also aims to provide application of the sodium alginate compounded apple polyphenol photothermal antibacterial dressing in preparation of wound repair medicines and medical appliances.
The above purpose of the invention is realized by the following technical scheme:
the photo-thermal antibacterial dressing is characterized in that iron ions and sodium alginate are crosslinked to construct a dressing matrix to load apple polyphenol.
In the invention, iron ions and sodium alginate are subjected to cross-linking reaction, so that a dressing matrix can be obtained by curing, and the apple polyphenol with antibacterial activity is further loaded to prepare the antibacterial dressing. Experiments prove that the components of the antibacterial dressing have a synergistic effect, and have a good photo-thermal circulation effect under the irradiation of near infrared light, so that the temperature of a wound can be increased, and the sterilization effect can be obviously enhanced; moreover, the loaded apple polyphenol has an antibacterial effect, and can be combined with a photothermal effect to synergistically improve the antibacterial effect, so that wound infection and inflammation are avoided on the premise of not adding antibiotics. In addition, the sodium alginate and the apple polyphenol have good effects of promoting wound healing, good biocompatibility and high biological safety, and are very suitable for repairing various wounds.
Preferably, the content of the apple polyphenol is 1-15% of the mass of the sodium alginate.
Further, the apple polyphenol comprises chlorogenic acid, catechin, epicatechin, apple condensed tannin, phlorizin, phloretin, and anthocyanidin. Can be purchased directly or prepared by extraction.
In addition, the invention also provides a preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing, which specifically comprises the following steps:
s1, uniformly mixing a sodium alginate solution and an apple polyphenol solution, defoaming, pre-freezing, and freeze-drying to obtain a prefabricated dressing;
and S2, soaking the prefabricated dressing obtained in the step S1 in a ferric salt solution for full reaction, washing, pre-freezing, and freeze-drying to obtain the dressing.
Further, in the step S1, after the sodium alginate solution and the apple polyphenol solution are uniformly mixed, the concentration of the sodium alginate solution is 5-15 mg/mL. Preferably, after the sodium alginate solution and the apple polyphenol solution are uniformly mixed, the concentration of the sodium alginate solution is 10mg/mL.
Furthermore, in the step S1, after the sodium alginate solution and the apple polyphenol solution are uniformly mixed, the concentration of the apple polyphenol is 0.05-2.25 mg/mL. Preferably, after the sodium alginate solution and the apple polyphenol solution are uniformly mixed, the concentration of the apple polyphenol is 0.25-1.25mg/mL.
Further, in step S1, the defoaming temperature is 1 to 10 ℃. Preferably, the defoaming time is 4-12 h. More preferably, the time for defoaming is 6h.
Preferably, a particular shape and size of the dressing is obtained by means of a mould.
Furthermore, in the steps S1 and S2, the pre-freezing temperature is-30 to-10 ℃, and the freeze-drying temperature is-50 to-30 ℃. Preferably, the pre-freezing time is 6-18 h, and more preferably, the pre-freezing time is 12h. Preferably, the freeze-drying time is 8 to 24 hours, and more preferably, the freeze-drying time is 12 hours.
Further, in step S2, the iron salt is selected from one or more of ferric chloride, ferric sulfate and ferric nitrate.
Further, in step S2, the iron ion concentration of the iron salt solution is 200-400 mM. Preferably, the iron salt solution has an iron ion concentration of 300mM.
Further, in the step S2, the time of the soaking reaction is 15 to 45min. Preferably, the soaking reaction time is 30min.
In addition, the invention also claims application of the sodium alginate composite apple polyphenol photothermal antibacterial dressing in preparation of wound repair medicines and medical appliances.
The invention has the following beneficial effects:
the components of the photo-thermal antibacterial dressing of the sodium alginate composite apple polyphenol have a synergistic effect, and under the irradiation of near infrared light, the photo-thermal antibacterial dressing has a good photo-thermal circulation effect, can improve the temperature of a wound, remarkably enhances the sterilization effect, and can also continuously resist bacteria by multiple times of irradiation; moreover, the loaded apple polyphenol has an antibacterial effect, and can be combined with a photothermal effect to synergistically improve the antibacterial effect, so that wound infection and inflammation are avoided on the premise of not adding antibiotics. In addition, the sodium alginate and the apple polyphenol have good effects of promoting wound healing, good biocompatibility and high biological safety, and are very suitable for repairing various wounds.
Drawings
Fig. 1 is an external view of an antibacterial dressing prepared in examples of the present invention and comparative examples.
FIG. 2 is a graph showing the photo-thermal temperature rise of the antibacterial dressings prepared in the examples of the present invention and the comparative examples under the irradiation of near infrared light of 808 nm.
FIG. 3 is a statistical chart of in vitro antibacterial effect test results of the antibacterial dressings prepared in the examples and comparative examples of the present invention on Escherichia coli under both light and non-light conditions.
Fig. 4 is a statistical chart of in vitro antibacterial effect test results of the antibacterial dressings prepared in the examples and comparative examples of the present invention on staphylococcus aureus under both light and non-light conditions.
Detailed Description
The invention is further described with reference to the drawings and the following detailed description, which are not intended to limit the invention in any way. The reagents, methods and apparatus employed in the present invention are conventional in the art, except as otherwise indicated.
Wherein Sodium Alginate (SA) is obtained from Shanghai Aladdin, and apple polyphenol (APP) is obtained from Shanxi Xia Biotech Co.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
EXAMPLE 1 photo-thermal antibacterial dressing SA/Fe/APP (0.25) of sodium alginate compounded apple polyphenol
The preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing specifically comprises the following steps:
s1, dissolving 400mg of SA in 20mL of ultrapure water to prepare a 2% SA solution, dissolving 10mg of APP in 20mL of ultrapure water to prepare an APP solution with the concentration of 0.5mg/mL, stirring and mixing the 10mL of SA solution and the 10mL of APP solution for 30min (the final concentration of APP is 0.25 mg/mL), adding 4mL of uniformly mixed solution into a mold, placing the mold at 4 ℃ for 6h for defoaming, placing the mold at minus 20 ℃ for pre-freezing for 12h after degassing, and freeze-drying at minus 40 ℃ for 12h to obtain a prefabricated dressing;
s2, using 5mL of 300mM FeCl for the prefabricated dressing obtained in the step S1 3 Soaking in water solution for 30min, cleaning with ultrapure water to colorless, pre-freezing at-20 deg.C for 12h, and freeze-drying at-40 deg.C for 12h to obtain sodium alginate compounded apple polyphenol photothermal antibacterial dressing SA/Fe/APP (0.25), the dressing appearance is shown in figure 1.
Example 2 sodium alginate composite apple polyphenol photothermal antimicrobial dressing SA/Fe/APP (0.5)
The preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing specifically comprises the following steps:
s1, dissolving 400mg of SA in 20mL of ultrapure water to prepare a 2% SA solution, dissolving 20mg of APP in 20mL of ultrapure water to prepare a 1mg/mL APP solution, then stirring and mixing the 10mL SA solution and the 10mL APP solution for 30min (the final concentration of APP is 0.5 mg/mL), adding 4mL of uniformly mixed solution into a mold, placing the mold at 4 ℃ for 6h for defoaming, placing the mold at-20 ℃ for pre-freezing for 12h after degassing, and carrying out freeze drying at-40 ℃ for 12h to obtain the prefabricated dressing;
s2, using 5mL 300mM FeCl for the prefabricated dressing obtained in the step S1 3 Soaking in water solution for 30min, cleaning with ultrapure water to colorless, pre-freezing at-20 deg.C for 12h, and freeze-drying at-40 deg.C for 12h to obtain sodium alginate compounded apple polyphenol photothermal antibacterial dressing SA/Fe/APP (0.5), the dressing appearance is shown in figure 1.
EXAMPLE 3 photo-thermal antibacterial dressing SA/Fe/APP (0.75) of sodium alginate compounded apple polyphenol
The preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing specifically comprises the following steps:
s1, dissolving 400mg of SA in 20mL of ultrapure water to prepare a 2% SA solution, dissolving 30mg of APP in 20mL of ultrapure water to prepare a 1.5mg/mL APP solution, stirring and mixing the 10mL of SA solution and the 10mL of APP solution for 30min (the final concentration of APP is 0.75 mg/mL), adding 4mL of uniformly mixed solution into a mold, placing the mold at 4 ℃ for 6h for defoaming, placing the mold at-20 ℃ for pre-freezing for 12h after degassing, and performing freeze drying at-40 ℃ for 12h to obtain a prefabricated dressing;
s2, using 5mL of 300mM FeCl for the prefabricated dressing obtained in the step S1 3 Soaking in water solution for 30min, cleaning with ultrapure water to colorless, pre-freezing at-20 deg.C for 12h, and freeze-drying at-40 deg.C for 12h to obtain sodium alginate compounded apple polyphenol photothermal antibacterial dressing SA/Fe/APP (0.75), the appearance of the dressing is shown in figure 1.
Example 4 sodium alginate compounded apple polyphenol photothermal antimicrobial dressing SA/Fe/APP (1.0)
The preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing specifically comprises the following steps:
s1, dissolving 400mg of SA in 20mL of ultrapure water to prepare a 2% SA solution, dissolving 40mg of APP in 20mL of ultrapure water to prepare a 2mg/mL APP solution, then stirring and mixing the 10mL SA solution and the 10mL APP solution for 30min (the final concentration of APP is 1 mg/mL), adding 4mL of uniformly mixed solution into a mold, placing the mold at 4 ℃ for 6h for defoaming, placing the mold at-20 ℃ for pre-freezing for 12h after degassing, and carrying out freeze drying at-40 ℃ for 12h to obtain a prefabricated dressing;
s2, using 5mL of 300mM FeCl for the prefabricated dressing obtained in the step S1 3 Soaking in water solution for 30min, cleaning with ultrapure water to colorless, pre-freezing at-20 deg.C for 12h, and freeze-drying at-40 deg.C for 12h to obtain sodium alginate compounded apple polyphenol photothermal antibacterial dressing SA/Fe/APP (1.0), the dressing appearance is shown in figure 1.
EXAMPLE 5 photo-thermal antibacterial dressing SA/Fe/APP (1.25) of sodium alginate compounded apple polyphenol
The preparation method of the sodium alginate composite apple polyphenol photothermal antibacterial dressing specifically comprises the following steps:
s1, dissolving 400mg of SA in 20mL of ultrapure water to prepare a 2% SA solution, dissolving 50mg of APP in 20mL of ultrapure water to prepare a 2.5mg/mL APP solution, stirring and mixing the 10mL of SA solution and the 10mL of APP solution for 30min (the final concentration of APP is 1.25 mg/mL), adding 4mL of uniformly mixed solution into a mold, placing the mold at 4 ℃ for 6h for defoaming, degassing, placing the mold at-20 ℃ for pre-freezing for 12h, and freeze-drying at-40 ℃ for 12h to obtain a prefabricated dressing;
s2, using 5mL of 300mM FeCl for the prefabricated dressing obtained in the step S1 3 Soaking in water solution for 30min, cleaning with ultrapure water to colorless, pre-freezing at-20 deg.C for 12h, and freeze-drying at-40 deg.C for 12h to obtain sodium alginate compounded apple polyphenol photothermal antibacterial dressing SA/Fe/APP (1.25), the dressing appearance is shown in figure 1.
Comparative example 1 sodium alginate antiseptic dressing SA/Fe
The preparation method of the sodium alginate antibacterial dressing specifically comprises the following steps:
s1, dissolving 400mg of SA in 20mL of ultrapure water to prepare a 2% SA solution, stirring and mixing 10mL of SA solution and 10mL of ultrapure water for 30min, adding 4mL of uniformly mixed solution into a mold, placing the mold at 4 ℃ for 6h for defoaming, placing the mold at-20 ℃ for pre-freezing for 12h after degassing, and freeze-drying at-40 ℃ for 12h to obtain a prefabricated dressing;
s2, using 5mL of 300mM FeCl for the prefabricated dressing obtained in the step S1 3 Soaking in water solution for 30min, cleaning with ultrapure water to colorless, pre-freezing at-20 deg.C for 12 hr, and freeze drying at-40 deg.CAnd 12h, obtaining the sodium alginate antibacterial dressing SA/Fe, wherein the appearance of the dressing is shown in figure 1.
Application example 1 evaluation of photothermal Properties of antibacterial dressing
And continuously irradiating the antibacterial dressing sample by using a light source of 808nm, recording the real-time temperature of the sample, drawing a temperature rise curve, and evaluating the photo-thermal performance of the dressing through the temperature rise curve after the dressing is irradiated by light.
The specific method comprises the following steps:
the dressings of example 1, example 2, example 3, example 4, example 5 and comparative example 1 prepared in examples and comparative example were prepared into a sample piece having a size of Φ 8mm × 2mm, placed in an EP tube, and 1mL of ultrapure water was added thereto, and the output power was 1.0W/cm 2 The 808nm point light source was used to irradiate the sample for 10min, the real-time temperature of the sample was recorded using a thermal imager, and the acquisition interval was 30s.
The experimental result is shown in fig. 2, and it can be seen from the graph that the temperature of the dressing added with the APP rises by 11-32 ℃ after being irradiated for 10min, the temperature rise is obviously greater than that of the dressing not added with the APP, and the temperature rise curve of the photo-thermal antibacterial dressing added with the APP shows time and concentration dependence.
In vitro antibacterial (E.coli) Effect of application example 2 antibacterial dressing
1. Experimental Material
Bacteria: escherichia coli (ATCC 25922).
Commercially available LB solid medium, LB liquid medium.
Examples and comparative examples the obtained antimicrobial dressing samples were prepared.
2. Experiment grouping
Light-shielded control group: blank control protected from light, i.e. bacteria were not treated with dressing and light.
Illumination control group: light blank, i.e. bacteria were not dressed but were light treated.
Light-shielding experimental group: the bacteria were treated with dressing only.
Illumination experimental group: the bacteria are treated by dressing and light irradiation.
3. Evaluation of antibacterial effect of antibacterial dressing on Escherichia coli
Adding the bacterial suspension into a 48-pore plate, adding an antibacterial dressing sample, carrying out no dressing treatment on a control group, carrying out illumination or light-shielding treatment on the pore plate, and then placing the pore plate in a constant-temperature shaking table for continuous culture for 12 hours; and finally, measuring the optical density value of the bacterial liquid at 600nm by using an enzyme-labeling instrument, and evaluating the survival rate of bacteria and the antibacterial effect of the dressing sample. The specific method comprises the following steps:
s1, dipping a single escherichia coli colony on a plate into 10mL LB liquid medium by using an inoculating loop, putting the mixture into a shaker at 37 ℃ and 100rpm for culturing for 12h, taking out the mixture, centrifuging the mixture at 3000rpm for 5min, removing supernatant, suspending the bacteria by using an appropriate amount of LB liquid medium to prepare 1.0 multiplied by 10 8 CFU/mL bacterial liquid is used for experiments;
s2, preparing the dressing into a sample piece with the size of phi 8mm multiplied by 2mm, and putting the sample piece into a container containing 0.5mL of 1 multiplied by 10 8 CFU/mL bacterial liquid in 48-hole plate;
s3, irradiating the illumination group for 10min by using a point light source with the wavelength of 808nm, and not processing the light-shading group; after the illumination is finished, 0.5mL of LB culture medium is supplemented to each experimental group, and the experimental groups are placed in a constant temperature shaking table with the temperature of 37 ℃ and the rpm of 100 for culture for 12 hours; and taking a proper amount of the bacterial liquid, and measuring the optical density value of the bacterial liquid at the 600nm position by using a microplate reader.
4. Results of the experiment
The results are shown in fig. 3, and it can be seen from the figure that the light-tight control group has a higher bacterial optical density value, which indicates that the survival condition of the bacteria is better, and the light-tight control group has no obvious difference in bacterial optical density value compared with the light-tight control group, which indicates that the survival rate of the bacteria is not affected by the light. The optical density value of the bacteria in the illumination experimental group is obviously lower than that of the light-shading control group, which shows that the survival rate of the bacteria in the illumination experimental group is lower, and the photothermal antibacterial dressing has a remarkable antibacterial effect on escherichia coli under the illumination condition.
Application example 3 in vitro antibacterial (s. Aureus) effect of the antibacterial dressing
1. Experimental materials
Bacteria: staphylococcus aureus (ATCC 25923).
Commercially available LB solid medium, LB liquid medium.
Examples and comparative examples the obtained antimicrobial dressing samples were prepared.
2. Experiment grouping
Light-shielded control group: blank control protected from light, i.e. bacteria were not treated with dressing and light.
Illumination control group: light blank, i.e. bacteria were not dressed but were light treated.
Light-protected experimental group: the bacteria were treated with dressing only.
Illumination experimental group: the bacteria are treated with dressing and light.
3. Evaluation of antibacterial effect of antibacterial dressing on staphylococcus aureus
Adding the bacterial suspension into a 48-pore plate, adding an antibacterial dressing sample, treating the control group without dressing, and placing the pore plate in a constant-temperature shaking table for continuous culture for 12 hours after illumination or shading treatment; and finally, measuring the optical density value of the bacterial liquid at 600nm by using an enzyme-labeling instrument, and evaluating the survival rate of bacteria and the antibacterial effect of the dressing sample. The specific method comprises the following steps:
s1, dipping a staphylococcus aureus single colony on a plate into 10mL LB liquid culture medium by using an inoculating loop, putting the mixture into a shaker at 37 ℃ and 100rpm for culturing for 12h, taking out the mixture, centrifuging the mixture for 5min at 3000rpm, removing supernatant, suspending the bacteria by using a proper amount of LB liquid culture medium to prepare 1.0 multiplied by 10 basis weight 8 CFU/mL bacterial liquid is used for experiments;
s2, preparing the dressing into a sample piece with the size of phi 8mm multiplied by 2mm, and putting the sample piece into a container containing 0.5mL of 1 multiplied by 10 8 CFU/mL bacterial liquid in a 48-pore plate;
s3, irradiating the illumination group for 10min by using a point light source with wavelength of 808nm, and not processing the light-shading group; after the illumination is finished, 0.5mL of LB culture medium is supplemented to each experimental group, and the experimental groups are placed in a constant-temperature shaking table at 37 ℃ and 100rpm for culture for 12 hours; and taking a proper amount of bacterial liquid, and measuring the optical density value of the bacterial liquid at 600nm by using a microplate reader.
4. Results of the experiment
As shown in fig. 4, the light-shielded control group has a higher bacterial optical density value, which indicates that the survival condition of the bacteria is better, and the light-shielded control group has no obvious difference in bacterial optical density value compared with the light-shielded control group, which indicates that the survival rate of the bacteria is not affected by light. The light intensity value of the bacteria in the light experiment group is obviously lower than that of the light-shading control group, which shows that the survival rate of the bacteria in the light experiment group is lower, and the photothermal antibacterial dressing has a remarkable antibacterial effect on staphylococcus aureus under the light condition.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The photo-thermal antibacterial dressing is characterized in that iron ions and sodium alginate are crosslinked to construct a dressing matrix to load apple polyphenol.
2. The photothermal antibacterial dressing according to claim 1, wherein the content of the apple polyphenol is 1-15% of the mass of sodium alginate.
3. The photothermal antimicrobial dressing of claim 1 wherein said apple polyphenols comprise chlorogenic acid, catechin, epicatechin, apple condensed tannin, phlorizin, phloretin, anthocyanins.
4. The preparation method of the sodium alginate compounded apple polyphenol photothermal antibacterial dressing as claimed in any one of claims 1 to 3, is characterized by specifically comprising the following steps:
s1, uniformly mixing a sodium alginate solution and an apple polyphenol solution, defoaming, pre-freezing, and freeze-drying to obtain a prefabricated dressing;
and S2, soaking the prefabricated dressing obtained in the step S1 in a ferric salt solution for full reaction, washing, pre-freezing, and freeze-drying to obtain the dressing.
5. The preparation method of claim 4, wherein in step S1, after the sodium alginate solution and the apple polyphenol solution are uniformly mixed, the concentration of the sodium alginate solution is 5-15 mg/mL.
6. The preparation method of claim 4, wherein in step S1, after the sodium alginate solution and the apple polyphenol solution are mixed uniformly, the concentration of apple polyphenol is 0.05-2.25 mg/mL.
7. The method according to claim 4, wherein the defoaming temperature in step S1 is 1 to 10 ℃.
8. The method according to claim 4, wherein the pre-freezing temperature is-30 to-10 ℃ and the freeze-drying temperature is-50 to-30 ℃ in the steps S1 and S2.
9. The preparation method according to claim 4, wherein in step S2, the iron salt is selected from one or more of ferric chloride, ferric sulfate and ferric nitrate.
10. The application of the sodium alginate compounded apple polyphenol photothermal antibacterial dressing as claimed in any one of claims 1 to 3 in the preparation of wound repair medicines and medical instruments.
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