CN104082528A - Feed additive and preparation method thereof - Google Patents
Feed additive and preparation method thereof Download PDFInfo
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- CN104082528A CN104082528A CN201310581921.7A CN201310581921A CN104082528A CN 104082528 A CN104082528 A CN 104082528A CN 201310581921 A CN201310581921 A CN 201310581921A CN 104082528 A CN104082528 A CN 104082528A
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Landscapes
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Abstract
Belonging to the field of feed additives, the invention discloses a feed additive and a preparation method thereof. The feed additive comprises the following raw materials by weight: 20-35 parts of an astragalus extract; 10-20 parts of a Codonopsis pilosula extract; 10-20 parts of a radix bupleuri extract; and 10-25 parts of a bacillus subtilis culture. The product provided by the invention has the characteristics of small usage, stable performance, use safety, and no incompatibility with other feed additives. The Chinese herbal extracts and a microbial agent are adopted in a reasonable proportion, enzymolysis and ethanol immersion extraction are employed to improve the extraction rates of amino acid, polysaccharide, trace elements and other beneficial ingredients in Chinese herbal medicines, and then by combining with the bacillus subtilis culture with high enzyme yield, digestion and absorption of beneficial Chinese herbal ingredients in feed can be promoted effectively. At the same time, the usable value of energy and protein of various feed resources is also enhanced, the production ability, the stress response and raising comprehensive benefits of livestock and poultry are strengthened, the animal daily gain and feed conversion rate are improved, and the drug usage is saved.
Description
Technical field:
The invention belongs to feed additive field, particularly contain high-efficiency feed additive of plurality of Chinese composition and preparation method thereof.
Background technology:
Along with the fast development of China's animal husbandry, the application of feed addictive is increasingly extensive, for ensure animal husbandry sound development, meet people the demand of animal food made a great contribution, but also brought series of problems simultaneously.Because most of additives belong to the medicine of chemical synthesis class, as hormone, antibiotic etc., in improving livestock and poultry output, livestock products medicine residual increases, the health that is directly endangering the mankind; And in modern livestock and poultry cultivation process, in order to prevent and treat Animal diseases, also often guarantee animal health with excessive antibiotic product, but excessive antibiotic use often causes the quality of animal meat product and product thereof to decline and antibiotic patience strengthens, and causes human health to be also subject to having a strong impact on of antibiotic problem by the transmission of food chain; People have recognized the negative effect that these chemical synthesis class additives, antibiotic etc. bring gradually.
Be derived from more natural natural materials and not only there is good nutritive peculiarity, also have simultaneously antiviral, anti-inflammatory, anti-oxidant, regulate the effects such as body's immunity, have broad application prospects; And Chinese herbal medicine is just with the mode of action, the good result of its uniqueness, noresidue, without the resistance to the action of a drug and pollution-free and be subject to the favor of numerous scientific research personnel, manufacturer.
At present, the Chinese herbal feed additive major part of putting on market is pulvis or powder, and its production technology falls behind, and production equipment is simple and crude, process coarse simple, kind is single, and using dosage is generally bigger than normal, and this has not only increased product cost, waste medicine, and having affected the nutrition-allocated proportion of feed, the existence of these problems also makes Chinese herbal feed additive in the market not meet the basic function principle of " trace, efficient " this feed addictive, is difficult to realize industrialization, standardization.
Therefore, develop and there is scientific matching, and successful contain multiple Chinese herbal and crude drugs preparations and can realize the feed addictive of " trace, efficient ", have very important significance for the development that promotes animal husbandry.
Summary of the invention:
The technical problem that the present invention solves is to provide a kind of high-efficiency feed additive product and preparation method thereof.
A kind of feed addictive, the raw material that comprises following parts by weight: 20-35 parts of Astragalus Root P.Es; 10-20 parts of Radix Codonopsis extracts; Bupleurum extract 10-20 part; 10-25 parts of bacillus subtilis cultures.
The production method of described feed addictive comprises the steps::
(1) preparation of Astragalus Root P.E: it is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, and controls temperature 70 C-90 DEG C, keeps 2-4h, with newborn acid for adjusting pH value be 5.5-6.8, be cooled to 45-60 DEG C, add mixed enzyme, enzymolysis 2-4h, add the mixture of 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 DEG C-78 DEG C, keep 3-4h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, 10-15 parts of pentosanases, 15-20 parts of beta amylases, acid protease 15-20 part.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.2.
(2) preparation of Radix Codonopsis extract: it is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, control temperature 70 C-90 DEG C, keep 2-4h, be down to 45-60 DEG C, with newborn acid for adjusting pH value be 5.5-6.8, add mixed enzyme, enzymolysis 2-4h, the mixture of interpolation 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is the 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 10-20 part, outer 1,4 beta-glucanase 10-20 part, 10-15 parts of beta-glucosidases, zytase 15-20 part, pentosanase 15-20 part, 10-15 parts of neutral proteinases.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.5.
(3) preparation of bupleurum extract: added the extraction of 3-6 times of weight absolute ethyl alcohol dippings of radix bupleuri after radix bupleuri is pulverized to 30-40 mesh sieves, control 30-45 DEG C of temperature, after 2-4 hours, adjusting temperature is 55-60 DEG C of 1-2 hour, concentrated, the dry ethanol extract that obtains of extract; In radix bupleuri residue after alcohol extract, add 75-85 DEG C of hot water, hot water addition is 2-4 times of radix bupleuri residue weight, and processing time 30-50 minute extracts 2-3 times continuously, by dry spraying after extract Vacuum Concentration, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged to drying and crushing, cross 45 mesh sieves, obtain bupleurum extract.
(4) preparation of bacillus subtilis culture: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, controls temperature and is 37-42 DEG C, aerlbic culture 19-24 hours, and throughput is 2.0m
3/ minute; Ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
The bacterial strain of the high temperature resistant AMS of product provided by the invention is specially bacillus subtilis (Bacillus subtilis) Li-2013-02.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute) on July 15th, 2013, and preserving number is CGMCC No.7926.
Feed addictive of the present invention compared with ratio of greater inequality example be: 25-30 parts of Astragalus Root P.Es; 13-18 parts of Radix Codonopsis extracts; 14-18 parts of bupleurum extracts; 15-20 parts of bacillus subtilis cultures.
The preparation method of described feed addictive is as follows: above-mentioned constitutive material is proportionally mixed to packaging.
Beneficial effect:
The Chinese herb astragalus adopting in the present invention: contain the various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper, there is invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, enhanced machine body immunity function, protect the liver, anti-ageing, anti-stress, hypotensive effect.
Radix Codonopsis: there is tonifying spleen and stomach and beneficial lung qi, beneficial gas effect to enrich blood; Radix Codonopsis also has excitation to nervous system, can strengthen Abwehrkraft des Koepers; Can also make peripheral vasodilation and reduce blood pressure, and can suppress adrenal boosting.
Radix bupleuri: there is the effect of antipyretic analgesic, heating due to typhoid fever, paratyphoid vaccine, Escherichia coli liquid, fermented milk, yeast etc. is had to trivial solution heat effect, and can make animal normal body temperature decline; Radix bupleuri has calm effect, is used for the treatment of the diseases such as the insomnia and dreamful sleep that interior hot agitation causes; Radix bupleuri has antagonism to central stimulant, therefore cough is had to certain therapeutic action; Radix bupleuri also infected by influenza, hepatitis viruse, vaccinia virus, I type poliomyelitis virus, herpesviral also produces effect, the effect that can also make phagocytic function enhancing, Natural killer activity enhancing, improve special viral antibody titre, raising lymphocyte turns core rate.
Feed addictive use amount of the present invention is few, stable performance, use safety, with other feed addictive all without incompatibility, adopt the Radix Astragali, Radix Codonopsis, bupleurum extract and bacillus subtilis culture rational proportion, the method of utilizing enzymolysis and alcohol dipping to extract has improved the amino acid in Chinese herbal medicine, polysaccharide, the recovery rate of the beneficiating ingredients such as trace element, utilize bacillus subtilis, consume rapidly the free oxygen in environment in alimentary canal, promote useful anaerobic bacteria growth, and produce the organic acids such as lactic acid, reduce enteron aisle pH value, improve gut flora, and effectively promote digesting and assimilating of Chinese herbal medicine beneficiating ingredient in feed, simultaneously bacillus subtilis thalline can also self synthetic proteins enzyme, the digestibility enzyme such as amylase, in alimentary canal, jointly play a role with endogenous enzymes, thereby that has improved the energy of all feeds resource and protein can utilization value, strengthened livestock and poultry production performance, stress performance and the comprehensive benefit of raising, improve animal daily gain and feed conversion rate, saved the use amount of feed.
Chinese herbal medicine in the present invention also has inhibitory action to the staphylococcus aureus of multiple coccus, bacillus and resistance, and bacillus subtilis thalline has growing of energy stimulating animal immune organ, activated lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, the function that strengthens cellular immunity and humoral immunity, the subtilin producing in growth course, polymyxins, nystatin, gramicidins isoreactivity material have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection; Therefore, the interaction to gut flora by Chinese herbal medicine extract and bacillus subtilis culture, has significantly reduced the intestines problem of animal, has reduced antibiotic use amount, has improved livestock and poultry body immunity, is natural green non-pollution animal feed.
In the bacillus subtilis culture containing in product, contain highly active amylase, amylase can provide effective decomposition utilization for starch material in feed, improves food utilization efficiency; The result of use of the enzyme that fire resistant alpha-diastase can effectively improve.Viable bacteria in bacillus subtilis culture, as probiotic component, also can effectively be improved the health of intestine microenvironment, promotes the raising of animal health level.
Product of the present invention is to improving the level of production of China's animal husbandry, and the effective utilization that improves feed resource, fully realizes aspects such as " trace, efficient " and all have very high using value
Detailed description of the invention:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Bacterial strain of the present invention is obtained through ultraviolet mutagenesis and nitrosoguanidine mutagenesis screening repeatedly by the wild mushroom of acid ground, and characteristic is that the enzyme activity of the high temperature resistant AMS of product is high, heat-resisting, acid resistance is strong.
The high temperature resistant AMS enzyme activity that bacterial strain produces is 30000-35000u/ml; Applicable temperature scope is 105-115 DEG C, and 110 DEG C of optimal reactive temperatures, at 110 DEG C of enzymes complete stability alive; Being suitable for pH value in reaction scope is 3.0-7.0, is 3.0 o'clock enzyme complete stabilities alive in pH value, and optimal reaction pH value is 4.2.
The bacterial strain of the high temperature resistant AMS of product provided by the invention is specially bacillus subtilis (Bacillus subtilis) Li-2013-02.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute) on July 15th, 2013, and preserving number is CGMCC No.7926.
Described bacterial strain feature is as follows:
Described bacterial strain colony colour on solid plate is milky, and dry tack free is opaque, and neat in edge, for having the aerobic bacteria of motility.Microscopy is elongated rod shape, and Gram's staining is positive.This bacterium can utilize citrate, and nitrate reductase, V-P test into positive.
Described bacillus subtilis (Bacillus subtilis) Li-2013-02 is obtained through UV-LiCl-dithyl sulfate Mutation screening by the bacillus subtilis Li-2013 that produces high temperature resistant AMS, specifically screens step as follows:
(1) preparation of bacteria suspension
By in mono-the Li-2013 growing after plate streaking separates bacterium colony access seed culture medium, 100r/min, cultivates after 12h for 40 DEG C, uses physiological saline washed twice after getting 1mL medium centrifugal, and in resuspended and 9mL physiological saline.
(2) UV-LiCl-dithyl sulfate complex mutation
Bacteria suspension is placed in to aseptic flat board, is 30cm in distance, stirs and irradiate 100s under the uviol lamp of power 15w.To after gradient dilution, coat lithium chloride flat board through the bacterium liquid irradiating, and contrast with the bacterium liquid dilution painting flat board without ultraviolet irradiation.Above-mentioned coating is dull and stereotyped uniformly, wrap with cloth or the newspaper of black, put 40 DEG C and cultivate 48h, on the flat board that grows bacterium colony, filtering out hydrolysis circle chooses to inclined-plane and preserves with colony diameter ratio the maximum, after purifying, be mixed with bacteria suspension, after gradient dilution, fully mix with dithyl sulfate stoste, and process 40min in 40 DEG C of concussions, the bacterium liquid of processing is coated to lithium chloride flat board after gradient dilution.
(3) primary dcreening operation of high-yield strains
Above-mentioned coating is dull and stereotyped uniformly, put 40 DEG C and cultivate 48h, on the flat board that grows bacterium colony, primary dcreening operation goes out hydrolysis circle and chooses to inclined-plane and preserve with colony diameter ratio the greater, obtains three strain bacterium Li-2013-01, Li-2013-02, Li-2013-03 after purifying
(4) shake flask fermentation sieves again
By the three strain bacterium Li-2013-01 that obtain, Li-2013-02, Li-2013-03 carries out shake flask fermentation in the 250mL shaking flask that contains 30mL fermentation medium, seed inoculum concentration 10% (V/V), 40 DEG C, 100r/min are cultivated 72h, and centrifuging and taking fermented supernatant fluid makes crude enzyme liquid.
(5) enzyme activity determination
The definition of Mei Huo unit: 1mL crude enzyme liquid, under 105 DEG C, pH4.2 condition, 1min liquefaction 1mg soluble starch,
Be 1 enzyme activity unit, represent with U/mL.
After measured, bacterial strain Li-2013-02, be stable superior strain, and enzyme work reaches 30000U/mL.
Described lithium chloride flat board: starch 1%, peptone 1%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%, lithium chloride 0.9%, agar 2%.
Described seed culture medium: dusty yeast 0.5%, peptone 1%, soluble starch 1%, NaCl1%.
Described fermentation medium: corn flour 5%-15%, beancake powder 4%-10%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%.
Described shaking flask condition of culture: this bacterium in the 250mL shaking flask that contains 30mL fermentation medium, inoculum concentration 10% (V/V), 100r/min, 40 DEG C of fermented and cultured 72h.
Described high-temperature resistant alpha-amylase, its zymologic property is as follows:
(1) this enzyme temperature accommodation is wider, and optimum temperature is between 100-110 DEG C, and 110 DEG C of following preserve, temperature stability is better, and it is poor more than 110 DEG C to preserve long-time temperature stability.
(2) this enzyme optimal reaction pH value is 4.2.Between pH value 3.0-7.0, all having high enzyme vigor, is 3.0 o'clock enzyme complete stabilities alive in pH value.
(3) enzymatic activity: by mutant strain Li-2013-02 provided by the present invention, the high temperature resistant AMS enzyme activity of preparation is 30000-35000U/ml.
Embodiment 1: preparation method is with example 3
A kind of feed addictive comprises the raw material of following parts by weight: 27 parts of Astragalus Root P.Es; 15 parts of Radix Codonopsis extracts; 16 parts of bupleurum extracts; 20 parts of bacillus subtilis cultures.
Embodiment 2:
The preparation of bacillus subtilis culture:
Adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: bacillus subtilis slant strains is accessed in 500 ml shake flasks to 100 milliliters of culture medium loading amounts, 180 revs/min of rotary shaking tables, 40 DEG C of cultivation temperature, incubation time 12 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of culture medium loading amounts, 100 revs/min of rotary shaking tables, 40 DEG C of cultivation temperature, incubation time 12 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: the first class seed pot by three grades of seeds taking 10% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 43 DEG C of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:1, tank pressure 0.05Mpa, incubation time 15 hours;
(5) fermented and cultured: first class seed pot bacterial classification is accessed to total measurement (volume) as 1.5 tons of secondary seed tanks taking 10% inoculum concentration, 1 ton of fermentation medium loading amount, 40 DEG C of condition of culture cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:1.5, tank pressure 0.05Mpa, incubation time 24 hours.
(6) preparation of culture: ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
Described culture medium composition: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO31%, pH6.8.
Described bacillus subtilis (Bacillus subtilis) preserving number is CGMCC No.7926.
Embodiment 3
The raw material that a kind of feed addictive comprises following parts by weight: 35 parts of Astragalus Root P.Es; 10 parts of Radix Codonopsis extracts; 18 parts of bupleurum extracts; 10 parts of bacillus subtilis cultures.
The production method of described feed addictive comprises the steps:
(1) preparation of Astragalus Root P.E: it is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 5 times of weight in container mixes, control 80 DEG C of temperature, keep 3h, be cooled to 55 DEG C, with newborn acid for adjusting pH value be 6, add mixed enzyme, enzymolysis 3h, the mixture of interpolation 2 times of weight ethanol of mixed material and propyl alcohol, control temperature to 68 DEG C and keep 4h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is 7% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: 20 parts of endo-beta-glucanases, 13 parts of zytases, 12 parts of pentosanases, 17 parts of beta amylases, 18 parts of acid proteases.
The mass ratio of described ethanol and propyl alcohol is 1:1.1.
(2) preparation of Radix Codonopsis extract: it is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 5 times of weight in container mixes, control 80 DEG C of temperature, keep 3h, be cooled to 50 DEG C, with newborn acid for adjusting pH value be 6, add mixed enzyme, enzymolysis 3h, the mixture of interpolation 3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 68 DEG C and keep 3h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is 7% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: 15 parts of endo-beta-glucanases, 15 parts of outer 1,4 beta-glucanases, 12 parts of beta-glucosidases, 18 parts of zytases, 17 parts of pentosanases, 12 parts of neutral proteinases.
The mass ratio of described ethanol and propyl alcohol is 1:1.
(3) preparation of bupleurum extract: radix bupleuri is pulverized and was added 5 times of weight absolute ethyl alcohols dippings of radix bupleuri after 35 mesh sieves and extract, control 38 DEG C of temperature, after 3 hours, adjust temperature and be 60 DEG C 2 hours, concentrated, the dry ethanol extract that obtains of extract; In radix bupleuri residue after alcohol extract, add 80 DEG C of hot water, hot water addition is 3 times of radix bupleuri residue weight, 40 minutes processing times, extract continuously 2 times, and by dry spraying after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged to drying and crushing, cross 45 mesh sieves, obtain bupleurum extract.
(4) bacillus subtilis culture preparation: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and controlling temperature is 40 DEG C, aerlbic culture 22 hours, throughput is 2.0m
3/ minute; Ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
Product result of use
The result of use test of the inventive example 3 feed addictives in weanling pig
Test method:
It is 120 of the healthy weanling pigs of (7.16 ± 0.15) kg that experimental animal is selected plant average weight, and statistical analysis body weight difference is not remarkable.Adopt single-factor Randomized Designs, by 120 healthy weanling pigs, by male and female half and half, be divided into 2 groups (control group and test group), every group of 6 repetitions, 10 pigs of each repetition.Test group is added the product of the present invention that embodiment 1 makes, and control group does not add product of the present invention.Feed continuously 30 days, compared with control group, result of the test shows, in weanling pig daily ration, adds this product, and the daily gain of piglet improves a lot than control group; Feedstuff-meat ratio has obvious reduction; Diarrhea rate has reduced by 85.7%.
The result of use test of the inventive example 1 feed addictive in milking sow
Carried out the feeding experiment of 21 days by a definite date on certain pig farm.Test adopts single factor contrast design, chooses at random 30 milking sows healthy, that a farrowing number is close with birth counterpoise, parity is 2 or 3 tires, is divided at random 2 groups (being test group and control group), every group of 15 repetitions.Wherein: test group daily ration adds this product being prepared into by embodiment 1, and control group adds common like product.Test shows: test group can improve 49.5% than control group weight of weaning litter, and a piglet daily gain improves 33.6%, and diarrhea rate reduces 57.3%, and the death rate reduces by 70.3%.
The above results shows that product of the present invention can improve sow and piglet body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.
Claims (8)
1. a feed addictive, the raw material that comprises following parts by weight: 20-35 parts of Astragalus Root P.Es; 10-20 parts of Radix Codonopsis extracts; 10-20 parts of bupleurum extracts; 10-25 parts of bacillus subtilis cultures; The preparation method of described bacillus subtilis culture is as follows: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and control temperature and be 37-42 DEG C, aerlbic culture 19-24 hours, throughput is 2.0m
3/ minute; Ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying; Described bacillus subtilis (Bacillus subtilis) preserving number is CGMCC No.7926.
2. feed addictive as claimed in claim 1, the raw material that comprises following parts by weight: 25-30 parts of Astragalus Root P.Es; 13-18 parts of Radix Codonopsis extracts; Bupleurum extract 14-18 part; Bacillus subtilis culture 15-20 part.
3. feed addictive as claimed in claim 1 or 2, is characterized in that, the preparation method of Astragalus Root P.E is as follows:
It is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, and controls temperature 70 C-90 DEG C, keeps 2-4h, be cooled to 45-60 DEG C, with newborn acid for adjusting pH value be 5.5-6.8, add mixed enzyme, enzymolysis 2-4h, add the mixture of 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature 60 C-78 DEG C, keep 3-4h, filter; Filtrate Vacuum Concentration postlyophilization;
Described mixed enzyme addition is 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 part, 15-20 parts of beta amylases, acid protease 15-20 part;
The mass ratio of described ethanol and propyl alcohol is 1:1-1.2.
4. feed addictive as claimed in claim 1 or 2, is characterized in that, the preparation method of Radix Codonopsis extract is as follows:
It is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, and controls temperature 70 C-90 DEG C, keeps 2-4h, be down to 45-60 DEG C, with newborn acid for adjusting pH value be 5.5-6.8, add mixed enzyme, enzymolysis 2-4h, add the mixture of 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 DEG C-78 DEG C, keep 3-4h, filter; Filtrate Vacuum Concentration postlyophilization;
Described mixed enzyme addition is 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 10-20 part, outer 1,4 beta-glucanase 10-20 part, beta-glucosidase 10-15 part, zytase 15-20 part, pentosanase 15-20 part, 10-15 parts of neutral proteinases;
The mass ratio of described ethanol and propyl alcohol is 1:1-1.5.
5. feed addictive as claimed in claim 1 or 2, is characterized in that, the preparation method of bupleurum extract is as follows:
After radix bupleuri is pulverized to 30-40 mesh sieves, added the extraction of 36 times of weight absolute ethyl alcohol dippings of radix bupleuri, and controlled 30-45 DEG C of temperature, after 2-4 hours, adjusting temperature is 55-60 DEG C, 1-2 hour, concentrated, the dry ethanol extract that obtains of extract; In radix bupleuri residue after alcohol extract, add 75-85 DEG C of hot water, hot water addition is 2-4 times of radix bupleuri residue weight, 30-50 minutes processing times, extract 2-3 time continuously, and by dry spraying after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged to drying and crushing, cross 45 mesh sieves, obtain bupleurum extract.
6. feed addictive as claimed in claim 1 or 2, the raw material that comprises following parts by weight: 35 parts of Astragalus Root P.Es; 10 parts of Radix Codonopsis extracts; 18 parts of bupleurum extracts; 10 parts of bacillus subtilis cultures.
7. feed addictive as claimed in claim 1 or 2, the production method of described feed addictive comprises the steps:
The constitutive material of described feed addictive is proportionally mixed to packaging;
(1) preparation of Astragalus Root P.E: it is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 5 times of weight in container mixes, control 80 DEG C of temperature, keep 3h, be cooled to 55 DEG C, with newborn acid for adjusting pH value be 6, add mixed enzyme, enzymolysis 3h, the mixture of interpolation 2 times of weight ethanol of mixed material and propyl alcohol, control temperature to 68 DEG C and keep 4h, filter; Filtrate Vacuum Concentration postlyophilization;
Described mixed enzyme addition is 7% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: 20 parts of endo-beta-glucanases, 13 parts of zytases, 12 parts of pentosanases, 17 parts of beta amylases, 18 parts of acid proteases;
The mass ratio of described ethanol and propyl alcohol is 1:1.1;
(2) preparation of Radix Codonopsis extract: it is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 5 times of weight in container mixes, control 80 DEG C of temperature, keep 3h, be cooled to 50 DEG C, with newborn acid for adjusting pH value be 6, add mixed enzyme, enzymolysis 3h, the mixture of interpolation 3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 68 DEG C and keep 3h, filter; Filtrate Vacuum Concentration postlyophilization;
Described mixed enzyme addition is 7% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: 15 parts of endo-beta-glucanases, 15 parts of outer 1,4 beta-glucanases, 12 parts of beta-glucosidases, 18 parts of zytases, 17 parts of pentosanases, 12 parts of neutral proteinases;
The mass ratio of described ethanol and propyl alcohol is 1:1;
(3) preparation of bupleurum extract: radix bupleuri is pulverized and was added 5 times of weight absolute ethyl alcohols dippings of radix bupleuri after 35 mesh sieves and extract, control 38 DEG C of temperature, after 3 hours, adjust temperature and be 60 DEG C 2 hours, concentrated, the dry ethanol extract that obtains of extract; In radix bupleuri residue after alcohol extract, add 80 DEG C of hot water, hot water addition is 3 times of radix bupleuri residue weight, 40 minutes processing times, extract continuously 2 times, and by dry spraying after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged to drying and crushing, cross 45 mesh sieves, obtain bupleurum extract;
(4) bacillus subtilis culture preparation: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and controlling temperature is 40 DEG C, aerlbic culture 22 hours, throughput is 2.0m
3/ minute; Ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
8. the preparation method of preparation described a kind of feed addictive as arbitrary in claim 1-7, described preparation method is as follows: the constitutive material of described feed addictive is proportionally mixed, pack.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293690A (en) * | 2014-05-27 | 2015-01-21 | 河南牧业经济学院 | Traditional Chinese medicinal micro-ecological composition, preparation thereof, and preparation method of preparation |
| CN109198301A (en) * | 2018-10-29 | 2019-01-15 | 南宁学院 | A kind of mudskipper parent population feed addictive |
| CN110604233A (en) * | 2019-10-30 | 2019-12-24 | 谷实农牧集团股份有限公司 | Medicinal plant micro-ecological feed for functional laying hens |
| CN112617019A (en) * | 2020-12-23 | 2021-04-09 | 宜兴市天石饲料有限公司 | Preparation method of polysaccharide type compound acidifier for feed |
| CN114982866A (en) * | 2022-07-12 | 2022-09-02 | 宁夏九盛牧业科技研究院(有限公司) | Premixed feed additive capable of improving diarrhea of young animals and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
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2013
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293690A (en) * | 2014-05-27 | 2015-01-21 | 河南牧业经济学院 | Traditional Chinese medicinal micro-ecological composition, preparation thereof, and preparation method of preparation |
| CN109198301A (en) * | 2018-10-29 | 2019-01-15 | 南宁学院 | A kind of mudskipper parent population feed addictive |
| CN110604233A (en) * | 2019-10-30 | 2019-12-24 | 谷实农牧集团股份有限公司 | Medicinal plant micro-ecological feed for functional laying hens |
| CN112617019A (en) * | 2020-12-23 | 2021-04-09 | 宜兴市天石饲料有限公司 | Preparation method of polysaccharide type compound acidifier for feed |
| CN114982866A (en) * | 2022-07-12 | 2022-09-02 | 宁夏九盛牧业科技研究院(有限公司) | Premixed feed additive capable of improving diarrhea of young animals and preparation method thereof |
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