CN103609858A - Feed additive and preparation method thereof - Google Patents
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Abstract
The invention discloses an efficient feed additive and a preparation method thereof and belongs to the field of feed additives. The feed additive comprises the following raw materials in parts by weight: 10-15 parts of a perillae extract, 5-10 parts of a yeast cytoderm extract, 15-30 parts of a bacillus subtilis culture, 5-8 parts of an astragalus membranaceus extract, 3-6 parts of a codonopsis pilosula extract and 2-6 parts of fructo-oligosaccharide. The feed additive is small in use amount, stable in performance and safe to use; the astragalus membranaceus extract, the codonopsis pilosula extract and the perillae extract are reasonably matched with the bacillus subtilis culture and the extraction rates of beneficial components including amino acids, polysaccharides, microelements and the like in Chinese herbal medicines are increased; bacillus subtilis is used for promoting the growth of beneficial anaerobic bacteria, reducing the PH (Potential of Hydrogen) value of intestinal tracts and improving intestinal flora; the digestion and the absorption of the beneficial components in Chinese herbal medicines in a feed are promoted effectively; the production performance and the stress performance of livestock and poultry are enhanced and the comprehensive benefits of feeding are increased; the daily gain of animals and the feed conversion rate are improved and the usage amount of the feed is saved.
Description
Technical field:
The invention belongs to feed additive field, particularly contain high-efficiency feed additive of plurality of Chinese composition and preparation method thereof.
Background technology:
Along with the fast development of China's animal husbandry, the application of feed addictive is increasingly extensive, for ensure animal husbandry sound development, meet people the demand of animal food made a great contribution, but also brought series of problems simultaneously.Because most of additives belong to the medicine of chemical synthesis class, as hormone, antibiotic etc., when improving livestock and poultry output, livestock products medicine residual increases, the health that is directly endangering the mankind; And in modern livestock and poultry cultivation process, in order to prevent and treat Animal diseases, also often with excessive antibiotic product, guarantee animal health, but excessive antibiotic use often causes the quality of animal meat product and product thereof to decline and the enhancing of antibiotic patience, the transmission by food chain causes human health to be also subject to having a strong impact on of antibiotic problem; People have recognized the negative effect that these chemical synthesis class additives, antibiotic etc. bring gradually.
Be derived from more natural natural materials and not only there is good nutritive peculiarity, also have simultaneously antiviral, anti-inflammatory, anti-oxidant, regulate the effects such as body's immunity, have broad application prospects; And Chinese herbal medicine is just with the mode of action, the good result of its uniqueness, noresidue, without the resistance to the action of a drug and pollution-free and be subject to the favor of numerous scientific research personnel, manufacturer.
At present, the Chinese herbal feed additive major part of putting on market is pulvis or powder, and its production technology falls behind, and production equipment is simple and crude, process coarse simple, kind is single, and using dosage is generally bigger than normal, and this has not only increased product cost, waste medicine, and having affected the nutrition-allocated proportion of feed, the existence of these problems also makes Chinese herbal feed additive in the market not meet the basic function principle of " trace, efficient " this feed addictive, is difficult to realize industrialization, standardization.
Therefore, develop and to there is scientific matching, and successful contain multiple Chinese herbal and crude drugs preparations and can realize the feed addictive of " trace, efficient ", for promoting the development of animal husbandry to have very important significance.
Summary of the invention:
The technical problem that the present invention solves is to provide a kind of high-efficiency feed additive product and preparation method thereof.
A feed addictive, the raw material that comprises following parts by weight: extractive of perilla 10-15 part, yeast cell wall extract 5-10 part, bacillus subtilis culture 15-30 part, Astragalus Root P.E 5-8 part; The 3-6 of Radix Codonopsis extract part; FOS 2-6 part; Aforementioned proportion is weight fraction ratio.
The production method of described feed addictive comprises the steps::
(1) preparation of Astragalus Root P.E: it is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, and controls temperature 70 C-90 ℃, keeps 2-4h, with newborn acid for adjusting pH value, be 5.5-6.8, be cooled to 45-60 ℃, add mixed enzyme, enzymolysis 2-4h, add the mixture of 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 ℃-78 ℃, keep 3-4h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is the 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 part, beta amylase 15-20 part, acid protease 15-20 part.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.2.
(2) preparation of Radix Codonopsis extract: it is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, control temperature 70 C-90 ℃, keep 2-4h, be down to 45-60 ℃, with newborn acid for adjusting pH value, be 5.5-6.8, add mixed enzyme, enzymolysis 2-4h, the mixture of interpolation 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 ℃-78 ℃ of maintenance 3-4h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is the 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 10-20 part, outer 1,4 beta-glucanase 10-20 part, beta-glucosidase 10-15 part, zytase 15-20 part, pentosanase 15-20 part, neutral proteinase 10-15 part.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.5.
(3) extractive of perilla preparation method is as follows: perilla seed added the extraction of perilla seed 7-10 times of weight absolute ethyl alcohol dipping after pulverizing 40-60 mesh sieve, control temperature 30-45 ℃, after 1-2 hour, adjusting temperature is 55-60 ℃ of 2-3 hour, concentrated, the dry ethanol extract that obtains of extract; In perilla seed residue after alcohol extract, add 75～85 ℃ of hot water, hot water addition is 3-6 times of perilla seed residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, and spraying after extract Vacuum Concentration is dry, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged to pulverizing, cross 60 mesh sieves, obtain extractive of perilla.
(4) preparation of bacillus subtilis culture: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and control temperature is 37-42 ℃, aerlbic culture 19-24 hour, throughput is 2.0m
3/ minute; Ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
The preparation method of described feed addictive is as follows: yeast cell wall extract and FOS were pulverized 50-60 mesh sieve, and above-mentioned crushed material and extractive of perilla and bacillus subtilis culture, Astragalus Root P.E, Radix Codonopsis extract are proportionally mixed to get to plant extracts composite feed additive.
The bacterial classification that the present invention adopts is as follows:
Bacillus subtilis (Bacillus subtilis) Li-2013-02, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2013 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, BeiJing, China city institute), preserving number is CGMCC No.7926.
Described strain characteristic is that the enzyme activity of the high temperature resistant AMS of product is high, heat-resisting, acid resistance is strong.
High temperature resistant AMS enzyme activity prepared by described bacterial strain is 30000-35000u/ml; Applicable temperature scope is 105-115 ℃, and 110 ℃ of optimal reactive temperatures, at 110 ℃ of enzymes complete stability alive; Applicable pH value in reaction scope is 3.0-7.0, in pH value, is 3.0 o'clock enzyme complete stabilities alive, and optimal reaction pH value is 4.2.
Described bacterial strain feature is as follows:
Described bacterial strain colony colour on solid plate is milky, and dry tack free is opaque, and neat in edge, for having the aerobic bacteria of motility.Microscopy is elongated rod shape, and Gram's staining is positive.This bacterium can utilize citrate, and nitrate reductase, V-P test into positive.
Described bacillus subtilis (Bacillus subtilis) Li-2013-02 is produced high temperature resistant AMS bacillus subtilis Li-2013 by a strain obtains through UV-LiCl-dithyl sulfate Mutation screening, specifically screens step as follows:
(1) preparation of bacteria suspension
By in the mono-bacterium colony of the Li-2013 growing after plate streaking separation access seed culture medium, 100r/min, cultivates after 12h for 40 ℃, uses physiological saline washed twice after getting 1mL medium centrifugal, and in resuspended and 9mL physiological saline.
(2) UV-LiCl-dithyl sulfate complex mutation
Bacteria suspension is placed in to aseptic flat board, is 30cm in distance, stirs and irradiate 100s under the uviol lamp of power 15w.To after gradient dilution, coat lithium chloride flat board through the bacterium liquid irradiating, and contrast with the bacterium liquid dilution painting flat board without ultraviolet irradiation.Above-mentioned coating is dull and stereotyped uniformly, with cloth or the newspaper of black, wrap, put 40 ℃ and cultivate 48h, on the flat board of bacterium colony, filter out hydrolysis circle and choose to inclined-plane and preserve with colony diameter ratio the maximum growing, after purifying, be mixed with bacteria suspension, after gradient dilution, fully mix with dithyl sulfate stoste, and process 40min in 40 ℃ of concussions, the bacterium liquid of processing is coated to lithium chloride flat board after gradient dilution.
(3) primary dcreening operation of high-yield strains
Above-mentioned coating is dull and stereotyped uniformly, put 40 ℃ and cultivate 48h, go out hydrolysis circle and choose to inclined-plane and preserve with colony diameter ratio the greater growing on the flat board of bacterium colony primary dcreening operation, after purifying, obtain three strain bacterium Li-2013-01, Li-2013-02, Li-2013-03
(4) shake flask fermentation sieves again
By the three strain bacterium Li-2013-01 that obtain, Li-2013-02, Li-2013-03 carries out shake flask fermentation in the 250mL shaking flask that contains 30mL fermentation medium, seed inoculum concentration 10% (V/V), 40 ℃, 100r/min are cultivated 72h, and centrifuging and taking fermented supernatant fluid makes crude enzyme liquid.
(5) enzyme activity determination
The definition of Mei Huo unit: 1mL crude enzyme liquid, under 105 ℃, pH4.2 condition, 1min liquefaction 1mg soluble starch,
Be 1 enzyme activity unit, with U/mL, represent.
After measured, bacterial strain Li-2013-02, be stable superior strain, and enzyme work reaches 30000U/mL.
Described lithium chloride is dull and stereotyped: starch 1%, peptone 1%, (NH) 2SO40.4%, K2HPO40.8%, CaCl20.2%, lithium chloride 0.9%, agar 2%.
Described seed culture medium: dusty yeast 0.5%, peptone 1%, soluble starch 1%, NaCl 1%.
Described fermentation medium: corn flour 5%-15%, beancake powder 4%-10%, (NH) 2SO40.4%, K2HPO40.8%, CaCl20.2%.
Described shaking flask condition of culture: this bacterium in the 250mL shaking flask that contains 30mL fermentation medium, inoculum concentration 10% (V/V), 100r/min, 40 ℃ of fermented and cultured 72h.
Described high-temperature resistant alpha-amylase, its zymologic property is as follows:
(1) this enzyme temperature accommodation is wider, and optimum temperature is between 100-110 ℃, and 110 ℃ of following preservations, temperature stability is better, and 110 ℃ above to preserve long-time temperature stabilities poor.
(2) this enzyme optimal reaction pH value is 4.2.Between pH value 3.0-7.0, all having high enzyme vigor, is 3.0 o'clock enzyme complete stabilities alive in pH value.
(3) enzymatic activity: by mutant strain Li-2013-02 provided by the present invention, the high temperature resistant AMS enzyme activity of preparation is 30000-35000U/ml.
Beneficial effect:
The Chinese herb astragalus adopting in the present invention: contain the various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper, there is invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, enhanced machine body immunity function, protect the liver, anti-ageing, anti-stress, hypotensive effect.
Radix Codonopsis: there is tonifying spleen and stomach and beneficial lung qi, the effect of beneficial gas to enrich blood; Radix Codonopsis also has excitation to nervous system, can strengthen Abwehrkraft des Koepers; Can also make peripheral vasodilation and reduce blood pressure, and can suppress adrenal boosting.
FOS refers to that 2～5 fructosyls are chain link, take an end group that glucosyl group is chain, and fructosyl → fructose connecting key of take links the carbohydrate forming as main body framework.Refer to that 1～4 fructosyl is with β-2,1 key is connected on the D-Fructose base of sucrose and the ketose (GF2) forming, Nystose (GF3), GF4 (GF4) and the sugarcane mixture of six sugar (GF5) really.FOS is a kind of good water-soluble dietary fiber, can also impel Bifidobacterium to breed rapidly, thereby suppresses the growth of harmful bacteria, safeguards gut flora balance; Can natural synthetic vitamin B1 in enteron aisle, B2, B6, B12, nicotinic acid and folic acid, thereby improve the function of enteron aisle, improve immunity and premunition.
Purple perilla: antibacterial action, wherein contained perillaldehyde, citral play main bacteriostasis, and staphylococcus aureus, fungi etc. is had to inhibitory action, and perilla ketone is as the active ingredient that promotes small bowel peristalsis in perilla leaf, can promote digestive juice secretion, strengthen gastrointestinal peristalsis.
Perilla seed of the present invention carries out pulverize and break cellular wall processing, make function factor in perilla seed realize full price stripping and high efficiency extraction utilization, adopt respectively organic solvent and hot water to realize wherein effective extraction of different efficacies composition, particularly control temperature section extraction and effectively improved especially extract yield, improved extraction efficiency and the quality of the extract of perilla seed.
In yeast cell wall extract, the functional form carbohydrate of yeast cells outer wall can, in conjunction with multiple mycotoxin, improve animal health by absorbing poisonous substance and pathogen to cell membrane.
Yeast cell wall extract is to take saccharomyces cerevisiae as raw material, and through breaking-wall cell, enzymolysis, separating-purifying and the refining class fungal extract forming of technique such as dry, finished product is generally the light grey powder to Dark grey.Studies confirm that, yeast cell wall is divided into 3 layers: outer is manna oligosacchride and protein conjugate, and intermediate layer is β-(1,3), β-(1,6) glucan, and internal layer is chitin.Its special configuration can form special complementary structure with multiple mycotoxin, thereby is combined firmly with multiple mycotoxin, and discharges outside animal body by enteron aisle.
Feed addictive use amount of the present invention is few, stable performance, use safety, with other feed addictive all without incompatibility, adopt the Radix Astragali, Radix Codonopsis, extractive of perilla and bacillus subtilis culture rational proportion, the method of utilizing enzymolysis and alcohol dipping to extract has improved the amino acid in Chinese herbal medicine, polysaccharide, the recovery rate of the beneficiating ingredients such as trace element, utilize bacillus subtilis, consume rapidly the free oxygen in digestion intestinal environment, promote useful anaerobic bacteria growth, and produce the organic acids such as lactic acid, reduce enteron aisle pH value, improve gut flora, and effectively promote digesting and assimilating of Chinese herbal medicine beneficiating ingredient in feed, simultaneously bacillus subtilis thalline can also self synthetic proteins enzyme, the digestibility enzyme such as amylase, in alimentary canal, jointly play a role with endogenous enzymes, thereby that has improved the energy of all feeds resource and protein can utilization value, strengthened livestock and poultry production performance, stress performance and the comprehensive benefit of raising, improve animal daily gain and feed conversion rate, saved the use amount of feed.
Chinese herbal medicine in the present invention also has inhibitory action to the staphylococcus aureus of multiple coccus, bacillus and resistance, and bacillus subtilis thalline has growing of energy stimulating animal immune organ, activated lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, the function that strengthens cellular immunity and humoral immunity, the subtilin producing in growth course, polymyxins, nystatin, gramicidins isoreactivity material have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection; Therefore, the interaction to gut flora by Chinese herbal medicine extract and bacillus subtilis culture, significantly reduced the intestines problem of animal, reduced antibiotic use amount, improve livestock and poultry body immunity, improved the security of animal meat product, improved the food utilization efficiency of animal, improving raise benefit, is natural green non-pollution animal feed.
Product of the present invention is to improving the level of production of China's animal husbandry, and the effective utilization that improves feed resource, fully realizes aspects such as " trace, efficient " and all have very high using value.
The specific embodiment:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1:
The preparation of bacillus subtilis culture:
Adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: bacillus subtilis slant strains is accessed in 500 ml shake flasks to 100 milliliters of culture medium loading amounts, 180 revs/min of rotary shaking tables, 40 ℃ of cultivation temperature, incubation time 12 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of culture medium loading amounts, 100 revs/min of rotary shaking tables, 40 ℃ of cultivation temperature, incubation time 12 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 10% inoculum concentration access total measurement (volume) is 150L, fermentation medium loading amount 100L, 43 ℃ of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:1, tank pressure 0.05Mpa, incubation time 15 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification be take to 10% inoculum concentration access total measurement (volume), 1 ton of fermentation medium loading amount, 40 ℃ of condition of culture cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:1.5, tank pressure 0.05Mpa, incubation time 24 hours.
(6) preparation of culture: ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
Described culture medium forms: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO3 1%, pH6.8.
Described bacillus subtilis (Bacillus subtilis) preserving number is CGMCC No.7926.
A kind of feed addictive comprises the raw material of following parts by weight: 13 parts of extractive of perilla, 7 parts of yeast cell wall extracts, 20 parts of bacillus subtilis cultures, 6 parts of Astragalus Root P.Es; 5 parts of Radix Codonopsis extracts; 5 parts of FOSs; Aforementioned proportion is weight fraction ratio; Bacillus subtilis (Bacillus subtilis) Li-2013-02 preserving number is CGMCC No.7926.
Embodiment 2:
A kind of feed addictive comprises the raw material of following parts by weight: 11 parts of extractive of perilla, 9 parts of yeast cell wall extracts, 25 parts of bacillus subtilis cultures, 7 parts of Astragalus Root P.Es; 4 parts of Radix Codonopsis extracts; 3 parts of FOSs; Aforementioned proportion is weight fraction ratio; Bacillus subtilis (Bacillus subtilis) Li-2013-02 preserving number is CGMCC No.7926.
Embodiment 3:
Feedstuff additive product production method described in example 2: yeast cell wall extract and FOS were pulverized 60 mesh sieves, is proportionally mixed to get plant extracts composite feed additive by above-mentioned crushed material and extractive of perilla and bacillus subtilis culture, Astragalus Root P.E, Radix Codonopsis extract.
The preparation of Astragalus Root P.E:
It is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 4 times of weight in container mixes, and controls 80 ℃ of temperature, keeps 2.5h, with newborn acid for adjusting pH value, be 6.2, be cooled to 50 ℃, add mixed enzyme, enzymolysis 3h, add the mixture of 1.5 times of weight ethanol of mixed material and propyl alcohol, control temperature to 70 ℃, keep 3h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is 8% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: 19 parts of endo-beta-glucanases, 12 parts of zytases, 13 parts of pentosanases, 16 parts of beta amylases, 17 parts of acid proteases.
The mass ratio of described ethanol and propyl alcohol is 1:1.2.
The preparation of Radix Codonopsis extract:
It is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 5 times of weight in container mixes, control 85 ℃ of temperature, keep 4h, be down to 55 ℃, with newborn acid for adjusting pH value, be 6.5, add mixed enzyme, enzymolysis 2h, the mixture of interpolation 1.5 times of weight ethanol of mixed material and propyl alcohol, control temperature to 65 ℃ and keep 4h, filter; Filtrate Vacuum Concentration postlyophilization.
Described mixed enzyme addition is 7% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: 16 parts of endo-beta-glucanases, 14 parts of outer 1,4 beta-glucanases, 14 parts of beta-glucosidases, 17 parts of zytases, 18 parts of pentosanases, 10 parts of neutral proteinases.
The mass ratio of described ethanol and propyl alcohol is 1:1.1.
Extractive of perilla preparation:
Perilla seed added the extraction of 8 times of weight absolute ethyl alcohol dippings of perilla seed after pulverizing 50 mesh sieves, controlled 40 ℃ of temperature, and after 2 hours, adjusting temperature is 55 ℃ of maintenances 3 hours, concentrated, the dry ethanol extract that obtains of extract; In perilla seed residue after alcohol extract, add 80 ℃ of hot water, hot water addition is 4 times of perilla seed residue weight, 45 minutes processing times, extract continuously 2 times, and spraying after extract Vacuum Concentration is dry, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged to pulverizing, cross 60 mesh sieves, obtain extractive of perilla.
The preparation of bacillus subtilis culture:
From inclined-plane switching, cultivate bacillus subtilis, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and controlling temperature is 40 ℃, aerlbic culture 20 hours, and throughput is 2.0m
3/ minute; Ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying.
Product result of use
The embodiment of the present invention 2 feed addictive result of use tests
Test method:
Select each 60 of healthy sow, piglet and boars that age in days, body weight are close, be all divided at random 2 groups (test group and control groups), test group is used product of the present invention, and control group does not add product of the present invention; The addition of product of the present invention is the 1-2% of feed consumption, feeds continuously 60 days.Compare with control group, use invention feed addictive can obtain following effect:
(1) number born of sow on average increases by 0.8;
(2) boar sperm count increases by 22%～29%, improves conception rate;
(3) before and after childbirth, prevention of sow constipation number of times reduces more than 32%;
(4) weanling pig weight ratio control group weight average increases by 10%;
(5) antibiotic use amount has reduced 80-95% the piglet phase, and diarrhea rate has reduced by 83%, and the death rate has reduced by 70%;
The above results shows that product of the present invention can improve sow, piglet and boar body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.Feed addictive of the present invention is of many uses, not only uses and meat swine rearing, is also applicable to the raising of chicken, duck, ox, sheep and aquatic livestock.
Claims (7)
1. a feed addictive, is characterized in that, described plant extracts composite feed additive consists of: extractive of perilla 10-15 part, yeast cell wall extract 5-10 part, bacillus subtilis culture 15-30 part, Astragalus Root P.E 5-8 part; The 3-6 of Radix Codonopsis extract part; FOS 2-6 part; Aforementioned proportion is weight fraction ratio; Described bacillus subtilis (Bacillus subtilis) Li-2013-02 preserving number is CGMCC No.7926.
2. a kind of feed addictive according to claim 1, is characterized in that, the preparation method of bacillus subtilis culture is as follows:
(1) first order seed is cultivated: bacillus subtilis slant strains is accessed in 500 ml shake flasks to 100 milliliters of culture medium loading amounts, 180 revs/min of rotary shaking tables, 40 ℃ of cultivation temperature, incubation time 12 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of culture medium loading amounts, 100 revs/min of rotary shaking tables, 40 ℃ of cultivation temperature, incubation time 12 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 10% inoculum concentration access total measurement (volume) is 150L, fermentation medium loading amount 100L, 43 ℃ of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:1, tank pressure 0.05Mpa, incubation time 15 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification be take to 10% inoculum concentration access total measurement (volume), 1 ton of fermentation medium loading amount, 40 ℃ of condition of culture cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:1.5, tank pressure 0.05Mpa, incubation time 24 hours;
(6) preparation of culture: ferment complete, zymotic fluid obtains bacillus subtilis culture after plate-frame filtering, concentrated, smart filter, freeze drying;
Described culture medium forms: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO3 1%, pH6.8.
3. a kind of feed addictive according to claim 1, it is characterized in that, described extractive of perilla preparation method is as follows: perilla seed added the extraction of perilla seed 7-10 times of weight absolute ethyl alcohol dipping after pulverizing 40-60 mesh sieve, temperature 30-45 ℃ was adjusted temperature after 1-2 hour is 55-60 ℃ of 2-3 hour, concentrated, the dry ethanol extract that obtains of extract; In perilla seed residue after alcohol extract, add 75～85 ℃ of hot water, hot water addition is 3-6 times of perilla seed residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, and spraying after extract Vacuum Concentration is dry, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged to pulverizing, cross 60 mesh sieves, obtain extractive of perilla.
4. a kind of feed addictive according to claim 1, is characterized in that, the preparation method of Astragalus Root P.E is as follows: it is below 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, and controls temperature 70 C-90 ℃, keeps 2-4h, be cooled to 45-60 ℃, with newborn acid for adjusting pH value, be 5.5-6.8, add mixed enzyme, enzymolysis 2-4h, add the mixture of 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature 60 C-78 ℃, keep 3-4h, filter; Filtrate Vacuum Concentration postlyophilization;
Described mixed enzyme addition is the 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 part, beta amylase 15-20 part, acid protease 15-20 part;
The mass ratio of described ethanol and propyl alcohol is 1:1-1.2.
5. a kind of feed addictive according to claim 1, is characterized in that, the preparation method of Radix Codonopsis extract is as follows: it is below 2 millimeters that raw material codonopsis pilosula powder is broken to particle diameter, the water that adds 3-6 times of weight in container mixes, and controls temperature 70 C-90 ℃, keeps 2-4h, be down to 45-60 ℃, with newborn acid for adjusting pH value, be 5.5-6.8, add mixed enzyme, enzymolysis 2-4h, add the mixture of 0.5-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 ℃-78 ℃, keep 3-4h, filter; Filtrate Vacuum Concentration postlyophilization;
Described mixed enzyme addition is the 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 10-20 part, outer 1,4 beta-glucanase 10-20 part, beta-glucosidase 10-15 part, zytase 15-20 part, pentosanase 15-20 part, neutral proteinase 10-15 part;
The mass ratio of described ethanol and propyl alcohol is 1:1-1.5.
6. a kind of feed addictive according to claim 1; It is characterized in that described plant extracts composite feed additive consists of: 11 parts of extractive of perilla, 9 parts of yeast cell wall extracts, 25 parts of bacillus subtilis cultures, 7 parts of Astragalus Root P.Es; 4 parts of Radix Codonopsis extracts; 3 parts of FOSs; Aforementioned proportion is weight fraction ratio; Bacillus subtilis (Bacillus subtilis) Li-2013-02 preserving number is CGMCC No.7926.
7. according to the preparation method of a kind of feed addictive described in claim 1 to 6, described preparation method is as follows: yeast cell wall extract and FOS were pulverized 50-60 mesh sieve, and above-mentioned crushed material and extractive of perilla and bacillus subtilis culture, Astragalus Root P.E, Radix Codonopsis extract are proportionally mixed to get to plant extracts composite feed additive.
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| CN201310589336.1A CN103609858A (en) | 2013-11-20 | 2013-11-20 | Feed additive and preparation method thereof |
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| CN104719632A (en) * | 2013-12-19 | 2015-06-24 | 天津工业大学 | Feed additive and preparation method thereof |
| CN105861355A (en) * | 2016-03-16 | 2016-08-17 | 孟丽辉 | Bacillus subtilis strain usable as probiotic preparation and fermentation process thereof |
| CN106173294A (en) * | 2016-07-13 | 2016-12-07 | 四川贡牛生物饲料有限公司 | Ruminant stomach animal is fattened growth-promoting feedstuff additive |
| CN106615808A (en) * | 2016-12-28 | 2017-05-10 | 梅州双胞胎饲料有限公司 | Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea |
| CN107494919A (en) * | 2017-10-11 | 2017-12-22 | 安徽省宿州市民辉养殖有限公司 | Big pig's feed and preparation method thereof in one kind |
| CN107996822A (en) * | 2017-11-10 | 2018-05-08 | 湖北华大瑞尔科技有限公司 | A kind of fermenting plant feed addictive of substitute antibiotics and preparation method thereof |
| CN108432946A (en) * | 2018-06-20 | 2018-08-24 | 江苏强森生物科技有限公司 | A kind of feed addictive and preparation method thereof of prevention animal diarrhea |
| CN110169498A (en) * | 2019-07-04 | 2019-08-27 | 湖南师范大学 | It is a kind of adjust animal and bird intestines length feed addictive and its application |
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| CN104719632A (en) * | 2013-12-19 | 2015-06-24 | 天津工业大学 | Feed additive and preparation method thereof |
| CN104206797A (en) * | 2014-07-15 | 2014-12-17 | 浙江大学 | Natural productpreparation for increasing content of CLA in milk and meat of ruminant |
| CN105861355A (en) * | 2016-03-16 | 2016-08-17 | 孟丽辉 | Bacillus subtilis strain usable as probiotic preparation and fermentation process thereof |
| CN106173294A (en) * | 2016-07-13 | 2016-12-07 | 四川贡牛生物饲料有限公司 | Ruminant stomach animal is fattened growth-promoting feedstuff additive |
| CN106615808A (en) * | 2016-12-28 | 2017-05-10 | 梅州双胞胎饲料有限公司 | Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea |
| CN107494919A (en) * | 2017-10-11 | 2017-12-22 | 安徽省宿州市民辉养殖有限公司 | Big pig's feed and preparation method thereof in one kind |
| CN107996822A (en) * | 2017-11-10 | 2018-05-08 | 湖北华大瑞尔科技有限公司 | A kind of fermenting plant feed addictive of substitute antibiotics and preparation method thereof |
| CN107996822B (en) * | 2017-11-10 | 2021-03-23 | 湖北华大瑞尔科技有限公司 | Fermented plant feed additive for replacing antibiotics and preparation method thereof |
| CN108432946A (en) * | 2018-06-20 | 2018-08-24 | 江苏强森生物科技有限公司 | A kind of feed addictive and preparation method thereof of prevention animal diarrhea |
| CN110169498A (en) * | 2019-07-04 | 2019-08-27 | 湖南师范大学 | It is a kind of adjust animal and bird intestines length feed addictive and its application |
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Application publication date: 20140305 |