CN102232998B - Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof - Google Patents
Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof Download PDFInfo
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Abstract
The invention provides a micro-ecological traditional Chinese medicine preparation for enhancing the immunity of livestock and poultry, and a preparation method thereof. The invention is characterized in that traditional Chinese medicines are combined with the modern microbial fermentation technology to develop a novel feed additive, i.e. traditional Chinese medicines such as astragalus root, eucommia, licorice, bupleurum and the like are directly fermented by two or more of sporeformer, microzyme, lactobacillus to obtain the product. The micro-ecological traditional Chinese medicine preparation provided by the invention has the efficacies of enhancing the body immunity of livestock and poultry, maintaining the gastrointestinal microbial flora balance of animals, preventing and treating alimentary and respiratory diseases of livestock and poultry, promoting healthy and quick growth of the animals, and improving the qualities of meat, egg and milk; the micro-ecological traditional Chinese medicine preparation has no toxic side effect, no pollution and no residue, can not generate drug resistance, and is suitable for use at each growth stage of the livestock and poultry; and the effect is stable and reliable and can meet the requirements of people for safety in production of livestock and poultry products. The micro-ecological traditional Chinese medicine preparation for enhancing the immunity of livestock and poultry can be increased in dose to be used as a veterinary medicine for application.
Description
Technical field
The invention belongs to feed additive field, relate to a kind of Chinese medicine microecological preparation that strengthens immunity of livestock and preparation method thereof, relate more specifically to the composition and method of making the sames of Chinese medicine behind probiotics fermention such as the Radix Astragali, the Cortex Eucommiae, Radix Glycyrrhizae, Rhizoma Zingiberis Recens.
Background technology
In the breeding process of poultry, the diseases such as digestive tract, respiratory tract often appear, and this has seriously affected the growth of poultry, even can cause poultry dead, causes very large loss for numerous raisers.The use of feeding antibiotic has improved the Production of Livestock and Poultry level greatly, and it promotes growth mechanism and effect to become common recognition, makes simultaneously animal husbandry scale and the intensive possibility that becomes.Antibiotic has been made significant contribution to aquaculture in the use history in more than 50 year in the past of China.Supply falls short of demand, urban and rural residents protein product source is deficient from the past animal products, and drug on the market to now domestic market meat, fowl, egg, milk, and realized in batches outlet.Yet the counter productive of its initiation has become the focus of concern.Fastbacteria growth and drug resistant gene that life-time service antibiotic or abuse of antibiotics cause shift, may cause the decline of humans and animals immunity of organisms, the increase of secondary dosage, drug residue, the dual and series of problems such as autogenous infection, sensitization, health is constituted a threat to.The animal food safety hidden danger that these factors cause enjoys consumer to pay close attention to.
Drug residue has become one of major reason that affects the export trade, and there is safety problem in the exported product major part of China, directly impact foreign exchange earning.On August 1st, 1996, European Union forbids also being contained in states such as Japan and Korea S., Russia subsequently from China's import broiler and aquatic products; Only January in August, 2000 to calendar year 2001, U.S. FDA has just been detained 634 batches of Chinese imported foods, and the overwhelming majority is because safety issue; On April 16th, 2002, the Chinese frozen meat that total value is 4,210,000 dollars is committed to the flames at Dutch Rui Dankeluodeshi, and its reason is to detect wherein to contain chloromycetin.Such example happens occasionally in recent years, has beaten alarm bell all for the aquaculture of China at every turn.
Meanwhile, along with the development of national economy and the progress of society, the people's living standard improves constantly, the progressively enhancing of self health consciousness, people more and more pay attention to the relation of diet and health, to meat, egg, milk consumption demand forward high-quality, nutrition, safe future development.
Summary of the invention
The object of the invention is to overcome the deficiency of above-mentioned technology, a kind of Chinese medicine microecological preparation that strengthens immunity of livestock and preparation method thereof is provided, Chinese medicine ingredients and multiple beneficial bacterium and the metabolite thereof with raising immunity contained in its inside.This Chinese medicine microecological preparation has significant immunoregulation effect, and has no side effect, pollution-free, noresidue, do not produce drug resistance.
Another object of the present invention provides the preparation method of the Chinese medicine microecological preparation of this enhancing immunity of livestock.
The present invention also provides a kind of feedstuff that comprises the Chinese medicine microecological preparation of this enhancing immunity of livestock.
To achieve these goals, the technical solution adopted in the present invention is as follows:
Described a kind of Chinese medicine microecological preparation that strengthens immunity of livestock, it is characterized in that the method that adopts Chinese medicine to pass through probiotics fermention obtains, wherein Chinese medicine comprises 1～10 part the Radix Astragali, 1～10 part the Cortex Eucommiae, 1～10 part Radix Glycyrrhizae and 1～10 part Radix Bupleuri by weight, and probiotic bacteria is arbitrarily two or more combination in sporeformer, yeast, the lactobacillus.
Described a kind of preparation method that strengthens the Chinese medicine microecological preparation of immunity of livestock may further comprise the steps:
(1) Chinese crude drug pretreatment
Choose Chinese crude drug, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross 80～120 mesh sieves, for subsequent use, wherein Chinese medicine comprises 1～10 part the Radix Astragali, 1～10 part the Cortex Eucommiae, 1～10 part Radix Glycyrrhizae and 1～10 part Radix Bupleuri by weight;
(2) preparation strain fermentating liquid
In the optimum medium that is inoculated in respectively in sporeformer, yeast, the lactobacillus separately, cultivate under suitable condition primary seed solution, carry out amplification culture in the secondary seed fermentation tank when each bacteria growing is transferred to respectively during to exponential phase, stop when reaching exponential phase cultivating, for subsequent use;
(3) herb fermenting
With the mix homogeneously according to a certain ratio of the Chinese crude drug after pulverizing, add water to water content between 50～70%, after soaking 12～24h, regulate between the pH value 6.0～7.5, at 121 ℃ of lower steam sterilization 15～20min, after the cooling, add the total strain liquid that accounts for Chinese crude drug gross weight 8%～20%, 28～37 ℃ fermented 24～72 hours, stirred once every 8h; After the fermentation ends, 35～50 ℃ of lower oven dry, namely get Chinese medicine microecological preparation.The Chinese medicine microecological preparation of described enhancing immunity of livestock is characterized in that described Chinese medicine also comprises one or more in 1～5 part of 1～5 part of Herba Ecliptae, 1～10 part of Fructus Ligustri Lucidi, 1～5 part of Radix Salviae Miltiorrhizae, 1～10 part of Fructus Lycii, 1～5 part of Semen Coicis, 1～5 part of Radix Scrophulariae, 1～5 part of Radix Saposhnikoviae, 1～5 part in Rhizoma Zingiberis Recens, 1～5 part of Radix Rehmanniae, 1～3 part of Fructus Schisandrae Chinensis, 1～10 part of Herba Taraxaci, 1～10 part of Rhizoma Dioscoreae and the Semen Allii Tuberosi by weight.
The preparation method of described Chinese medicine microecological preparation is characterized in that total strain liquid is by with two or more is mixed and made into seed mixture liquid by equal proportion arbitrarily in sporeformer secondary seed solution, yeast secondary seed solution, the lactobacillus secondary seed solution.
Described a kind of Chinese medicine microecological preparation that strengthens immunity of livestock is characterized in that described sporeformer comprises Bacillus licheniformis, bacillus subtilis and/or bacillus cereus.
Described a kind of Chinese medicine microecological preparation that strengthens immunity of livestock is characterized in that described yeast comprises Candida utilis and/or saccharomyces cerevisiae.
Described a kind of Chinese medicine microecological preparation that strengthens immunity of livestock is characterized in that described lactobacillus comprises enterococcus faecalis, lactobacillus lactis, bacillus acidophilus, lactobacillus casei, lactobacillus lactis and/or Lactobacillus bulgaricus.
Described a kind of feedstuff that strengthens the Chinese medicine microecological preparation of immunity of livestock is characterized in that, comprises the constituent of following part by weight: the Chinese medicine microecological preparation 0.02～0.2% of described enhancing immunity of livestock and adjuvant 99.98～99.8%.
The invention provides a kind of Chinese medicine microecological preparation that strengthens immunity of livestock and preparation method thereof, have the following advantages:
1, Chinese medicine microecological preparation of the present invention can strengthen the immunocompetence of animal body, keep animal intestines and stomach microorganism species balance, effectively prevent and treat livestock and poultry alimentary tract and respiratory tract disease, promote livestock birds health, rapidly growth, the meat, eggs and milk quality better reduces the effects such as cultivation input;
2, the present invention's Chinese medicine microecological preparation of strengthening immunity of livestock each growth stage of being adapted at livestock and poultry animal is used;
3, the present invention adopts probiotic bacteria direct fermentation Chinese medicine, has protected active ingredient of Chinese herbs to exempt from destruction, produces new drug effect;
Microbial fermentation is to carry out under the comparatively gentle condition such as room temperature, normal pressure, and active component exempts from destruction in the Chinese medicine so can protect to greatest extent.And the larger active component of the number molecular weight in the Chinese medicine becomes small-molecule active substance and directly utilized after fermentation, and this has improved the drug effect of Chinese medicine greatly; The enzyme of microorganisms carries out structural modification to active ingredient of Chinese herbs, generates secondary metabolite, thereby has produced new active substance, thereby have new drug effect;
4, the present invention adopts multiple probiotic mixed fermentation Chinese medicine, can produce such as cellulase, amylase, protease etc., so that the substance of the difficult absorptions such as the cellulose in the Chinese medicine, lignin is fully degraded, Effective Component of Chinese Medicine is discharged to greatest extent, simultaneously cellulose conversion can be become oligosaccharide, promote the growth of probiotics in the animal gastrointestinal tract; Under the effect of the various enzymes that produce, the effective ingredient in the feedstuff is easier to be absorbed by animal simultaneously;
5, the present invention utilizes the novel fodder additive of the interaction development of Chinese medicine and probiotic bacteria, and unrivaled advantage is arranged, and it has and has no side effect, pollution-free, noresidue, do not produce the security features such as drug resistance;
6, it is reliable that the present invention strengthens the effect stability of immunity of livestock Chinese medicine microecological preparation, can satisfy people to the requirement of meat, egg, milk safety in production;
7, the present invention strengthens the immunity of livestock Chinese medicine microecological preparation and can pass through the raising using dosage, uses as veterinary drug.
, it should be understood that described embodiment only for the present invention is described, rather than limit the scope of the invention by any way more specific description the present invention by following examples.
The specific embodiment
Embodiment 1
Chinese medicine: 10 parts of the Radixs Astragali, 6 parts of the Cortexs Eucommiae, 6 parts in Radix Glycyrrhizae, 3 parts of Radix Bupleuri, 5 parts of Herba Ecliptaes.
Fermentation technology:
Bacillus licheniformis, bacillus subtilis are inoculated into respectively in the common nutrient broth medium, and at 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 5%, cultivation temperature is 28～35 ℃, rotating speed is 200rpm, when sporeformer content reaches the 109CFU/ml left and right sides, stop fermentation, for subsequent use;
Saccharomyces cerevisiae is inoculated in the malt extract medium, and 28 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 6%, cultivation temperature is 28～35 ℃, and rotating speed is 200rpm, when yeast content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Choose above-mentioned each Chinese crude drug, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
Take by weighing Chinese medicinal powder after the pulverizing by above-mentioned prescription, add water to water content at 50-60%, behind the mix homogeneously, at room temperature soak 24h, regulate about pH7.0, at 121 ℃ of lower steam sterilization 15～20min, after the cooling, add respectively the Bacillus licheniformis liquid account for Chinese medicine gross weight 5%, 5% bacillus subtilis bacterium liquid and 5% saccharomyces cerevisiae bacterium liquid, at 28～35 ℃ of fermentation 36h, stir once every 8h; When total viable count reaches 1.0 * 10
8～9Stop fermentation during CFU/ml, 45 ℃ of lower vacuum dryings make Chinese medicine microecological preparation.
Embodiment 2
Chinese medicine: 8 parts of the Radixs Astragali, 6 parts of the Cortexs Eucommiae, 10 parts in Radix Glycyrrhizae, 3 parts of Radix Bupleuri, 4 parts of Herba Ecliptaes, 5 parts of Fructus Ligustri Lucidi, 3 parts of Radix Salviae Miltiorrhizaes, 10 parts of Fructus Lyciis, 6 parts of Semen Coicis, 3 parts of Radix Scrophulariaes, 2 parts of Radix Saposhnikoviaes, 6 parts of Rhizoma Dioscoreaes, 5 parts of Semen Allii Tuberosi, 3 parts of Radix Rehmanniae, 6 parts of Herba Taraxacis.
Fermentation technology:
Bacillus licheniformis is inoculated in the common nutrient broth medium, and at 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank with inoculum concentration 3%, cultivation temperature is 30～32 ℃, and rotating speed is 200rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Candida utilis is inoculated in the malt extract medium, and 28 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank with inoculum concentration 8%, cultivation temperature is 30～32 ℃, and rotating speed is 200rpm, when yeast content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Lactobacillus lactis and Lactobacillus bulgaricus are inoculated into respectively in the MRS culture medium, and 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 5%, cultivation temperature is 30～32 ℃, and rotating speed is 200rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Choose above-mentioned each Chinese crude drug, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
Take by weighing Chinese medicinal powder after the pulverizing by above-mentioned prescription, add water to water content at 60-70%, behind the mix homogeneously, at room temperature soak 24h, regulate PH6.5～6.9, at 121 ℃ of lower steam sterilization 20min, after the cooling, add respectively the Bacillus licheniformis liquid account for Chinese medicine gross weight 2.5%, 2.5% Candida utilis bacterium liquid, 2.5% lactobacillus lactis bacterium liquid and 2.5% Lactobacillus bulgaricus bacterium liquid, at 30～32 ℃ of fermentation 48h, stir once every 8h; When total viable count reaches 1.0 * 10
8～9Stop fermentation during CFU/ml, 40 ℃ of lower vacuum dryings make Chinese medicine microecological preparation.
Embodiment 3
Chinese medicine: 5 parts of the Radixs Astragali, 8 parts of the Cortexs Eucommiae, 10 parts in Radix Glycyrrhizae, 2 parts of Radix Bupleuri, 2 parts in Rhizoma Zingiberis Recens, 4 parts of Herba Ecliptaes, 5 parts of Fructus Ligustri Lucidi, 3 parts of Radix Salviae Miltiorrhizaes, 9 parts of Fructus Lyciis, 5 parts of Semen Coicis.
Fermentation technology:
Bacillus licheniformis is inoculated in the common nutrient broth medium, and at 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank with inoculum concentration 3%, cultivation temperature is 30 ± 2 ℃, and rotating speed is 200rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Candida utilis and saccharomyces cerevisiae are inoculated in the malt extract medium, and 28 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank with inoculum concentration 8%, cultivation temperature is 30 ± 2 ℃, and rotating speed is 200rpm, when yeast reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Enterococcus faecalis, lactobacillus casei and Lactobacillus bulgaricus are inoculated into respectively in the MRS culture medium, and 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into amplification culture in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 5%, cultivation temperature is 30 ± 2 ℃, and rotating speed is 200rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
Choose above-mentioned each Chinese crude drug, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
Take by weighing Chinese medicinal powder after the pulverizing by above-mentioned prescription, add water to water content 60%, behind the mix homogeneously, at room temperature soak 24h, regulate pH6.5～6.9, at 121 ℃ of lower steam sterilization 20min, after the cooling, add respectively the lactobacillus casei bacterium liquid of the Bacillus licheniformis liquid account for Chinese medicine gross weight 2.5%, 2.5% Candida utilis bacterium liquid, 2.5% saccharomyces cerevisiae liquid, 2.5% enterococcus faecalis bacterium liquid and 2.5% and 2.5% Lactobacillus bulgaricus bacterium liquid, at 30 ± 2 ℃ of fermentation 40h, stir once every 8h; When total viable count reaches 1.0 * 10
8～9Stop fermentation during CFU/ml, 50 ℃ of lower vacuum dryings make Chinese medicine microecological preparation.
Embodiment 4
Feedstuff comprises the constituent of following mass percent: the Chinese medicine microecological preparation 0.05% and the adjuvant 99.95% that strengthen immunity of livestock.The addition that namely strengthens the Chinese medicine microecological preparation of immunity of livestock in 1 ton of feedstuff is 500g.Feedstuff can specifically comprise the constituent of following mass fraction: by 0.05 part of the Chinese medicine microecological preparation of the prepared enhancing immunity of livestock of the embodiment of the invention 2,55 parts of Semen Maydis powder, 1.1 parts of wheat-middlings, 30 parts of bean cake, 9 parts of cottonseed meal, 1 part in corn bean white lead, 0.6 part of oils and fats, 0.9 part of hydrogen calcium, 1.1 parts of stone powders, 0.3 part of Sal, 0.3 part of lysine, 0.3 part of methionine, 0.05 part of choline chloride, 0.02 part of compound cellulose pre-mixing agent, 0.28 part of compound microelement pre-mixing agent.
Embodiment 5
A kind of feedstuff (piglet is used) that contains the Chinese medicine microecological preparation that strengthens immunity of livestock comprises the constituent of following mass percent: the Chinese medicine microecological preparation 0.12% and the adjuvant 99.88% that strengthen immunity of livestock.The addition that namely strengthens the Chinese medicine microecological preparation of immunity of livestock in 1 ton of feedstuff is 1200g.Feedstuff can specifically comprise the constituent of following mass fraction: by 0.12 part of the Chinese medicine microecological preparation of the prepared enhancing immunity of livestock of the embodiment of the invention 3,59 parts of Semen Maydiss, 16 parts of puffing Semen sojae atricolor, 13 parts of dehulled soybean meal, 4 parts of imported fish meals, 3.88 parts of glucoses, 4 parts of 4% piglet premixes.
Embodiment 6
A kind of feedstuff (nursing sow is used) that contains the Chinese medicine microecological preparation that strengthens immunity of livestock comprises the constituent of following mass percent: the Chinese medicine microecological preparation 0.1% and the adjuvant 99.9% that strengthen immunity of livestock.The addition that namely strengthens the Chinese medicine microecological preparation of immunity of livestock in 1 ton of feedstuff is 1000g.Feedstuff can specifically comprise the constituent of following mass fraction: by 0.1 part of the Chinese medicine microecological preparation of the prepared enhancing immunity of livestock of the embodiment of the invention 1,61.9 parts of Semen Maydiss, 22 parts of soybean meal, 10 parts in Testa Tritici, 1 part of imported fish meal, 1 part in Oleum Glycines, 4 parts of 4% nursing sow premix material.
Below by concrete test example the beneficial effect of the Chinese medicine microecological preparation of enhancing immunity of livestock of the present invention is described.
Test example 1
This test example has been studied the Chinese medicine microecological preparation of enhancing immunity of livestock of method preparation of embodiment 2 to the impact of fertility performance and the premunition of ablactational baby pig.
1 materials and methods
1.1 time and the place of test
Tested on May 26,12 days to 2010 May in 2010 and finish on Nan Gezhuan pig farm, Beijing.
1.2 the selection of experimental animal and grouping
Select the source identical, age in days is identical, body weight close (about 6-7 kilogram), 120 of Du of 21 ages in days that are in a good state of health wean * large * long three way cross commodity piglet are divided into 5 processed group at random, and each processed group is established 2 repeating groups, 12 pigs of each repeating groups, male and female half and half.
1.3 experimental period
Experimental period is 14 days, piglet 21 age in days to 35 ages in days.
Claims (12)
1.-plant the Chinese medicine microecological preparation that strengthens immunity of livestock, it is characterized in that the method that adopts Chinese medicine to pass through probiotics fermention obtains, wherein Chinese medicine comprises 1～10 part the Radix Astragali, 1～10 part the Cortex Eucommiae, 1～10 part Radix Glycyrrhizae and 1～10 part Radix Bupleuri by weight, and probiotic bacteria is arbitrarily two or more combination in sporeformer, yeast, the lactobacillus.
2. according to right 1 described a kind of preparation method that strengthens the Chinese medicine microecological preparation of immunity of livestock, may further comprise the steps:
(1) Chinese crude drug pretreatment
Choose Chinese medicine, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross 80～120 mesh sieves, for subsequent use, wherein Chinese medicine comprises 1～10 part the Radix Astragali, 1～10 part the Cortex Eucommiae, 1～10 part Radix Glycyrrhizae and 1～10 part Radix Bupleuri by weight;
(2) preparation strain fermentating liquid
In the optimum medium that is inoculated in respectively in sporeformer, yeast, the lactobacillus separately, cultivate under suitable condition primary seed solution, carry out amplification culture in the secondary seed fermentation tank when each bacteria growing is transferred to respectively during to exponential phase, stop when reaching exponential phase cultivating, for subsequent use;
(3) fermentation
With the mix homogeneously according to a certain ratio of the Chinese crude drug after pulverizing, add water to water content between 50～70%, after soaking 12～24h, regulate between the pH value 6.0～7.5, at 121 ℃ of lower steam sterilization 15～20min, after the cooling, add the total strain liquid that accounts for Chinese crude drug gross weight 8%～20%, 28～37 ℃ fermented 24～72 hours, stirred once every 8h; After the fermentation ends, 35～50 ℃ of lower oven dry, namely get Chinese medicine microecological preparation.
3. according to claim 1, the Chinese medicine microecological preparation of 2 described enhancing immunity of livestock, it is characterized in that described Chinese medicine also comprises one or more in 1～5 part of 1～5 part of Herba Ecliptae, 1～10 part of Fructus Ligustri Lucidi, 1～5 part of Radix Salviae Miltiorrhizae, 1～10 part of Fructus Lycii, 1～5 part of Semen Coicis, 1～5 part of Radix Scrophulariae, 1～5 part of Radix Saposhnikoviae, 1～5 part in Rhizoma Zingiberis Recens, 1～5 part of Radix Rehmanniae, 1～3 part of Fructus Schisandrae Chinensis, 1～10 part of Herba Taraxaci, 1～10 part of Rhizoma Dioscoreae and the Semen Allii Tuberosi by weight.
4. the preparation method of Chinese medicine microecological preparation according to claim 2 is characterized in that total strain liquid is by with two or more is mixed and made into seed mixture liquid by equal proportion arbitrarily in sporeformer secondary seed solution, yeast secondary seed solution, the lactobacillus secondary seed solution.
5. according to claim 1,2 described a kind of Chinese medicine microecological preparations that strengthen immunity of livestock, it is characterized in that described sporeformer comprises Bacillus licheniformis, bacillus subtilis and/or bacillus cereus.
6. according to claim 1,2 described a kind of Chinese medicine microecological preparations that strengthen immunity of livestock, it is characterized in that described yeast comprises Candida utilis and/or saccharomyces cerevisiae.
7. according to claim 1,2 described a kind of Chinese medicine microecological preparations that strengthen immunity of livestock, it is characterized in that described lactobacillus comprises enterococcus faecalis, lactobacillus lactis, bacillus acidophilus, lactobacillus casei, lactobacillus lactis and/or Lactobacillus bulgaricus.
8. feedstuff that comprises the Chinese medicine microecological preparation of claim 1,2 or 4 each described enhancing immunity of livestock, it is characterized in that, comprise the constituent of following part by weight: the Chinese medicine microecological preparation 0.02～0.2% of described enhancing immunity of livestock and adjuvant 99.98～99.8%.
9. feedstuff that comprises the Chinese medicine microecological preparation of enhancing immunity of livestock claimed in claim 3, it is characterized in that, comprise the constituent of following part by weight: the Chinese medicine microecological preparation 0.02～0.2% of described enhancing immunity of livestock and adjuvant 99.98～99.8%.
10. feedstuff that comprises the Chinese medicine microecological preparation of enhancing immunity of livestock claimed in claim 5, it is characterized in that, comprise the constituent of following part by weight: the Chinese medicine microecological preparation 0.02～0.2% of described enhancing immunity of livestock and adjuvant 99.98～99.8%.
11. feedstuff that comprises the Chinese medicine microecological preparation of enhancing immunity of livestock claimed in claim 6, it is characterized in that, comprise the constituent of following part by weight: the Chinese medicine microecological preparation 0.02～0.2% of described enhancing immunity of livestock and adjuvant 99.98～99.8%.
12. feedstuff that comprises the Chinese medicine microecological preparation of enhancing immunity of livestock claimed in claim 7, it is characterized in that, comprise the constituent of following part by weight: the Chinese medicine microecological preparation 0.02～0.2% of described enhancing immunity of livestock and adjuvant 99.98～99.8%.
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