The third part (10 mL of rumen fluid) was used for variable bacteria counts (cellulolytic, proteolytic, and
amylolytic) using the roll-tube technique [20].
Five bacterial groups were selected and classified according to their metabolic ability: bacteria capable of producing lactic acid (lactic acid bacteria) and those capable of degrading cellulose (cellulolytic bacteria), lipids (lipolytic bacteria), proteins (proteolytic bacteria) or starches (
amylolytic bacteria).
Molecular technique for yeast strain that was containing
amylolytic, cellulolytic, and a-glucosidase enzymes was constructed (30).
According to T.Ashokvardhan; it was found that among the 25 Actinomycetes, 96% (24 strains) were
amylolytic, 88% were proteolytic and 44% (11 strains) exhibited lipolytic activity 55, Payal found that during primary screening, a total of 8090% isolates showed cellulase activity 8, Mohanta, found 18% isolates as cellulase producing organism (47).
Digestive proteolytic and
amylolytic activities of Helicoverpa armigera in response to feeding on different soybean cultivars.
Protozoa are competing for substrate with
amylolytic bacteria, and protozoa ferment starch more slowly than
amylolytic bacteria (Owens et al., 1998; Nagaraja et al., 1992), resulting in reduced rumen starch digestibility and pushing starch digestion to the intestines (Mendoza et al., 1993).
Nowadays the enzymes from hyperthermophiles are widely used in industries (Sabir et al., 2017) and especially are replacing the use of other
amylolytic enzymes in starch industry.
cerevisiae: For determination of
amylolytic activity, 1g nutrient broth (NB), 2g agar, 1g starch were used to prepare the amylase media plates.
These groups of microorganisms account for the soil functional diversity and are directly associated with its health and fertility (i.e., cellulolytic, proteolytic,
amylolytic, phosphate solubilizers, nitrogen-fixing bacteria with glucose as a carbon source, nitrogen-fixing bacteria with malate as a carbon source, Actinobacteria, total fungi, and heterotrophic bacteria).
Guyot, "Kinetic growth parameters of different
amylolytic and non-amylolytic lactobacillus strains under various salt and pH conditions," Bioresource Technology, vol.