A 57-amino acid pea chloroplast transit peptide was fused to the N-terminus of the sequence to target protein expression to the
amyloplast, a nonpigmented organelle responsible for starch synthesis and storage.
Starch is found in
amyloplast in granules form and accounts for 65-70% of grain (Li et al., 1999).
Starch staining of the hypocotyls with 1-KI solution shows absence of
amyloplast granules in suppressors grown on sucrose free medium.
It occurs as granules in the chloroplast of green leaves and the
amyloplast of seeds, pulses, and tubers.
A statolith is an object in the plant cell now thought to be an
amyloplast, which belongs to a class of colorless plastids called leucoplasts.
The most noticeable correlated event we have noted is the asynchronous appearance of
amyloplast in microspores in the loculus.
The translocator protein is like a door that allows glucose to move out of the
amyloplast. Scientists want to shut that door, and this mutation does that.
Wasserman of Rutgers University, whose work focuses on the synthesis of amylose and amylopectin, and the molecular biology of the enzymes that catalyze chain elongation, insertion of branch points and spatial distribution of enzymes within the corn endosperms
amyloplast.
Starch staining of the hypocotyl with I-KI solution shows almost an absence of
amyloplast granules in the suppressors grown in sucrose free medium.
In addition, at 4[degrees]C
amyloplasts sedimented normally in the gps mutants, indicating that the GPS loci affect an aspect of the gravity signal perception/transduction pathway that occurs after
amyloplast sedimentation (perception).
They suggested that this inverse relationship may be due to differences in
amyloplast membranes, such as the composition of membrane lipids.