WO2020107875A1

WO2020107875A1 - Acer pentaphyllum cutting propagation method

Info

Publication number: WO2020107875A1
Application number: PCT/CN2019/092188
Authority: WO
Inventors: 何程相; 罗雪梅; 谢松林; 马建华; 朱晓菲; 王小辉; 向明剑; 丁龙梅; 许艺
Original assignee: 四川七彩林科股份有限公司
Priority date: 2018-11-30
Filing date: 2019-06-21
Publication date: 2020-06-04
Also published as: CN109247148A

Abstract

An acer pentaphyllum cutting propagation method, comprising the following steps: acquiring acer pentaphyllum branches, trimming into short branches having a length of 5-8 cm to serve as cuttings, trimming, soaking the bases of the cuttings into water as soon as possible, and spraying clear water to wet the leaves; preparing a mixed substrate from perlite and peat using a volume ratio of 6:4, disinfecting the mixed substrate, and adding same into deepened trays having tray covers; soaking the bases of the cuttings in rooting liquid for 0.5h, inserting into the mixed substrate, and compacting immediately, holes being in one-to-one correspondence to the cuttings; putting the deepened trays into a simple culture chamber, controlling the temperature to be 22-25°C, keeping humidity in the deepened tray at 100% and illuminating intensity in a range of 700-1200 lx, irrigating the cuttings and the nearby mixed substrate using 40 mL of rooting liquid 3 times in total at a time interval of 3d; and 10d after cuttage, spraying a disinfectant once every other week until rooting of all cuttings is achieved. With the described method, components of the rooting liquid are simple, costs are low, steps are simple, there is no complexity, and a high cuttage rooting rate and survival rate are achieved.

Description

Five-leaf maple cutting propagation method

Technical field

The invention belongs to the technical field of plant propagation and propagation, and relates to a method for cutting propagation of maple leaflet.

Background technique

Acer pentaphyllum (Dicer) is a deciduous tree of the genus Acer of the Aceraceae family. It can reach a height of 10 m. It has palmate compound leaves with 4 to 7 leaflets, usually 5 leaflets. It is endemic to Sichuan, China. Due to its unique leaf type and brilliant colors, this plant is one of the most ornamental maple species in the world. It is the same name as Davidia involucrata, the famous ornamental tree species in China, in the western horticultural community. According to the IUCN Endangered Rating Standard (IUCN 1994), this species is already a critically endangered species. According to the literature, there are only more than 500 strains of Acer palmatum in the wild, which belong to 4 populations, and these populations are being subject to human grazing, felling and Serious threats such as the construction of hydropower stations are on the verge of extinction, and emergency rescue is urgently needed.

At present, there are few reports on the research of Acer palmatum, mainly focusing on the assessment of its genetic variation, the study of resource status and the exploration of seedling and afforestation technology, and the propagation technology of Acer palmatum is only about seed propagation and tissue culture. Yes, there is no report on cutting propagation research. Application for the Chinese invention patent published as CN 108142284A "A method of tissue culture and rapid propagation of Acer palmatum" and application of the Chinese invention patent published as CN 107107398A "A method for the rapid propagation of maple branches" All shoots of A. pentaphyllum with buds are used as explant materials. After primary culture, subculture and rooting culture, A. pentaphyllum seedlings are obtained with a survival rate of more than 80%, but the process is cumbersome and the plants added to the medium There are many kinds of growth regulators, and the cultivation process requires high environment.

Summary of the invention

The object of the present invention is to provide a method for cutting propagation of Acer palmatum, which has simple rooting fluid composition, low cost, simple steps, and is not cumbersome. The cutting rooting rate and survival rate are high, and large-scale industrial seedling raising can be carried out.

To achieve the above objectives, the present invention adopts the following technical solutions:

A cutting propagation method of maple leaf five leaves includes the following steps:

Step a: Collect the five-leaf maple branches and trim them to a short branch of 5-8 cm in length as cuttings. Immediately after pruning, immerse the cuttings into the water and spray the leaves with fresh water;

Step b: Remove the leaves less than 3cm from the base of the cuttings, keep the leaves at the top of the cuttings, and keep 2 to 3 nodes in the whole cuttings;

Step c: Using perlite and peat with a volume ratio of 6:4 as a mixed matrix, sterilize the mixed matrix and insert it into a deepened tray with a tray cover;

Step d: Use a wooden stick with a diameter of 0.3 cm to perforate the mixed substrate in the deepening tray, the depth of the hole is 3 cm, soak the base of the cutting ear in the rooting solution for 0.5 h after inserting it into the mixed substrate, and then According to reality; the composition of the rooting fluid includes: 50-100 mg/L NAA-NA and 100-400 mg/L IBA-K ⁺ ;

Step e: Put the deepening tray into the simple culture room, control the temperature at 22-25°C, keep the humidity in the deepening tray at 100%, the light intensity 700～1200lx, and irrigate the cutting medium and its surrounding mixed substrate every 3 days 40ml of rooting solution was irrigated 3 times; after cutting for 10 days, disinfectant was sprayed once every other week until all roots were rooted.

Further, the branch in step a is the top part of the current semi-lignified branch or the middle and lower part of the branch of the 3-year-old seedling of Acer pennatum and the 1-year-old tissue culture potted seedling.

Further, the branch in step a is the top of the current semi-lignified branch of the one-year-old tissue culture potted seedling of Acer palmatum.

Further, the specific steps of sterilizing the mixed substrate in step c are: spraying and mixing the mixed substrate with a 500-fold solution of 75% wettable chlorothalonil powder to ensure that the mixed substrate is covered with mulch after pouring for a week, Then spray it with clean water for 2 to 3 times and then put it into the deepening tray.

Further, the composition of the rooting solution in step d includes: 50 mg/L NAA-NA and 200 mg/L IBA-K ⁺ .

Further, in step e, the disinfectant is an 800 times solution of 75% wettable chlorothalonil powder.

In the present invention, NAA-NA is sodium a-naphthalene acetate, which belongs to auxin plant growth regulator, promotes the formation of adventitious roots and roots, and can be used to promote rooting of seeds, rooting of cuttings and fibrous roots of solanaceous crops.

In the present invention, IBA-K ⁺ is potassium indole butyrate. The potassium salt of indole butyrate is more stable than indole butyrate and is completely soluble in water. It has the functions of promoting cutting rooting, crop growth, yield increase, and seed germination. effect.

Compared with the prior art, the beneficial effects of the present invention are:

1. In the present invention, the top of the branches or the middle and lower parts of the branches of 3-year-old A. pentaphylla seedlings or 1-year-old tissue culture potted seedlings are selected as cuttings. In a mixed matrix composed of perlite and peat, the A. pentaphylla is obtained by cutting propagation. Cutting seedlings; and through comparative experiments, it was obtained that the rooting rate of the top of the branches of the 1-year-old tissue culture potted seedlings was higher, and the base of the cuttings was in NAA-NA (50mg/L) + IBA-K ⁺ (200mg/L) solution After soaking for 0.5h, the cuttings were cut. After cutting, the cuttings and their surrounding substrates were watered with 40ml of NAA-NA (50mg/L) + IBA-K ⁺ (200mg/L) solution, which was obtained by irrigating 3 times. Has the highest rooting rate of 91.67%.

2. The cutting propagation method of the present invention is simpler in composition and lower in cost than the tissue culture propagation method. Compared with the tissue culture propagation method, it requires the steps of primary, secondary and rooting cultivation. The steps used are simple, not cumbersome, and cutting Root tips began to appear in about 20 days. After 60 days, most of the root systems of A. pentaphyllum can clump the substrate, and the survival rate and rooting rate are high. It is not affected by seasonal climate changes and natural disasters, and large-scale factory seedlings can be carried out.

BRIEF DESCRIPTION

Fig. 1 shows the top of the half-woody branches of the year of the annual tissue culture potted seedlings collected in the present invention.

FIG. 2 is a diagram showing that the root system of the annual seedling half-wooden branches of the annual tissue-cultured potted seedlings is slightly cut and cultivated for 60 days, and the root system of the seedlings and the substrate are clumped together.

Fig. 3 shows the growth of the root system of the cutting seedlings after soaking the rooting liquid before cutting and immersing the rooting liquid for 60 days after the cutting of the semi-lignified branches in the present invention.

detailed description

The following embodiments are used to illustrate the present invention, but are not used to limit the protection scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art. Unless otherwise specified, the test methods in the following examples are conventional methods.

Example 1

Step a: Collect the top of the semi-woody branches of the three-year-old A. pentaphyllum seedlings and trim them to a length of 5-8 cm as cuttings. The tops of the branches need to grow well and be free of diseases and insect pests. After trimming, immerse the cuttings into the water immediately And spray the blades with fresh water.

Step b: Remove the leaves less than 3cm from the base of the cuttings, keep the leaves at the top of the cuttings, and keep 2 to 3 nodes in the whole cuttings.

Step c: Use perlite and peat with a volume ratio of 6:4 as the mixed matrix, and sterilize the mixed matrix into a 50-hole deepening plug tray with a plug tray cover; the specific steps for the disinfection of the mixed matrix are: use 75% The 500 times solution of wettable chlorothalonil powder was sprayed on the mixed substrate and mixed evenly, to ensure that the mixed substrate was covered with a mulch after pouring, and then sprayed with clean water for 2 to 3 times and then loaded into the deepening tray.

Step d: Use a wooden stick with a diameter of about 0.3 cm to perforate the mixed substrate in the deepening tray at a depth of about 3 cm. According to a hole and a spike, soak the base of the cutting ear in the rooting solution for 0.5 h and insert it into the mixed substrate. Press as you go; the composition of the rooting solution includes: 50 mg/L NAA-NA and 200 mg/L IBA-K ⁺ .

Step e: Put the deepening tray into the simple culture room, control the temperature at 22-25°C, keep the humidity in the deepening tray at 100%, the light intensity 700～1200lx, and irrigate the cutting medium and its surrounding mixed substrate every 3 days 40ml of rooting solution was irrigated 3 times; after 10 days of cutting, disinfectant was sprayed once every other week until all roots were rooted. The disinfectant was 800 times solution of 75% wettable chlorothalonil powder.

Example 2

Step a: Collect the semi-lignified branches of the one-year-old tissue culture potted seedlings of the five-leaf maple seedlings to survive, and trim them to 5-8 cm as cuttings. The tops of the branches are required to grow well and free from diseases and insect pests. The base is immersed in water, and the leaves are wetted with clean water.

Example 3

Step a: Collect the mid-lower part of the current half-lignified branches of the one-year-old tissue culture potted seedlings of the five-leaf maple seedlings, and trim them to 5-8 cm as cuttings. The middle and lower parts of the branches must grow well and be free from pests and diseases. The base is immersed in water, and the leaves are wetted with clean water.

Step e: Put the deepening tray into the simple culture room, control the temperature at 22-25°C, keep the humidity in the deepening tray at 100%, the light intensity 700～1200lx, and irrigate the cutting medium and its surrounding mixed substrate every 3 days 40ml of rooting solution was irrigated 3 times; after 10 days of cutting, disinfectant was sprayed once every other week until all roots were rooted. The disinfectant was 800% solution of 75% wettable chlorothalonil powder.

Comparative test 1

The top half of the current year's semi-woody branches (as shown in Figure 1) and the mid-lower part of the branches and the top half of the current year's semi-woody branches of the 3-year-old seedlings were collected separately. For the ear picking source and ear picking position of the cutting method, 8 cutting ears are processed in each embodiment, and repeated three times. The other steps are the same as those in Embodiments 1 to 3. The root tip began to appear about 20 days after cutting. After 60 days, most of the root system of Acer palmatum can entrap the substrate. All treatments were counted for rooting after 60 days, including average rooting rate, average root number and average root length. The results are shown in Table 1. One-year-old tissue culture pot seedlings of the current year's semi-lignified branches were slightly cut at the top and cultivated for 60 days. The roots of the cut seedlings and the substrate were clumped as shown in Figure 2.

Table 1 Statistical results of experiments on different ear picking sources and ear picking parts

It can be seen from Table 1 that the average semi-woody branch crest of the current year using the 1-year-old tissue culture potted seedlings is slightly higher than that of the 3-year-old solid seedlings, and the average rooting rate is higher; The top of the half-woody branches of the tissue culture pot seedlings is slightly higher than the middle and lower parts of the half-woody branches of the current year tissue culture pot seedlings, so when cutting propagation of maple five-leaf maple, 1 The annual semi-lignified branches of the annual tissue culture potted seedlings are slightly crested.

Comparative test 2

The annual half-lignified branches of the one-year-old tissue culture potted seedlings were collected for cuttings. In Comparative Test 2, only the cuttings were immersed, and no rooting solution was poured into the substrate. Therefore, in this experiment, except for the proportion of regulators and cuttings In addition to the different methods, the materials, substrates, cutting methods and post-environment management are all consistent with the steps in Example 2. In the experiment, 20 cuttings were treated for each number and repeated 3 times. Among them, the test number 1 was the clean water control treatment. All treatments were rooted after 60 days, including the average rooting rate, average root number and average root length, the results are shown in Table 2.

Table 2 The effects of plant growth regulator types and concentrations on the rooting of Acer palmatum

It can be seen from Table 2 that when the concentration of NAA-NA is 50 mg/L and the concentration of IBA-K ⁺ is 200 mg/L, the average rooting rate is the highest, reaching 58.33%, which is preferable.

Comparative test 3

Select the top of the current year's semi-lignified shoots of 1-year-old tissue culture potted seedlings as cuttings, and perform three treatments before and after inserting into the mixed substrate to investigate the effects of different treatments on the rooting of A. pentaphyllum cuttings. The three treatments are as follows: ( 1) Soak the base of cuttings in NAA-NA (50mg/L) + IBA-K ⁺ (200mg/L) for 0.5h and then cut; (2) Soak the base of cuttings in NAA-NA (50mg/L) + IBA- In K ⁺ (200mg/L), the cuttings were cut after 0.5h. After cuttings, 40ml of NAA-NA (50mg/L) + IBA-K ⁺ (200mg/L) solution was irrigated to the cuttings and the surrounding substrate every 3d. 3 times; (3) Immerse the cuttings base in clear water for 0.5h and then cut them. After cuttings, pour 40ml of NAA-NA (50mg/L) + IBA-K ⁺ (200mg/ L) The solution is watered 3 times in total. In the experiment, 12 cuttings were treated each and repeated 3 times. In the comparative test, except that the treatment methods before and after inserting the cuttings into the mixed substrate are different, the source and location of the material, the cutting cutting mixed substrate, and the later management are the same as in Example 2. All treatments were counted for rooting after 60 days, including average rooting rate, average root number and average root length. The results are shown in Table 3. The growth of the root system of the one-year-old tissue culture potted seedlings was soaked with rooting solution before the cuttings were soaked with rooting solution before cutting and watered with rooting solution for 60 days.

Table 3 The effect of different treatments on the rooting of Acer quinquefolium cuttings

It can be seen from Table 3 that the cutting base is immersed in NAA-NA (50mg/L) + IBA-K ⁺ (200mg/L) solution for 0.5h and then cuttings are carried out. The cuttings and their surrounding substrates are cut every 3 days after cuttings Irrigation of 40ml of NAA-NA (50mg/L) + IBA-K ⁺ (200mg/L) solution, a total of 3 times of irrigation treatments, the highest average rooting rate was 91.67%.

Comparing Table 2 and Table 3, it can be seen that the method of combining the two methods of soaking the base of the cutting ear with rooting solution and irrigating the rooting agent with the surrounding matrix of the cutting ear and the surrounding substrate, compared with the method of soaking the base of the cutting ear only with rooting solution The rooting rate of Acer lobata cuttings has been greatly improved.

The above-mentioned embodiments are only preferred embodiments of the present invention, and are only used to explain the present invention, not to limit the scope of the present invention. For those skilled in the art, of course, it can be based on the disclosure in this specification The technical content can easily be made into other embodiments by replacement or change. Therefore, any changes and improvements made on the principle of the present invention should be included in the patent application scope of the present invention.

Claims

A cutting propagation method of maple leaflet, characterized in that it includes the following steps:

Step a: Collect the five-leaf maple branches and trim them to a short branch of 5-8 cm in length as cuttings. Immediately after pruning, immerse the cuttings into the water and spray the leaves with fresh water;

Step b: Remove the leaves less than 3cm from the base of the cuttings, keep the leaves at the top of the cuttings, and keep 2 to 3 nodes in the whole cuttings;

Step c: Using perlite and peat with a volume ratio of 6:4 as a mixed matrix, sterilize the mixed matrix and insert it into a deepened tray with a tray cover;

Step d: Use a wooden stick with a diameter of 0.3 cm to perforate the mixed substrate in the deepening tray, the depth of the hole is 3 cm, soak the base of the cutting ear in the rooting solution for 0.5 h after inserting it into the mixed substrate, and then According to reality; the composition of the rooting solution includes: 50-100 mg/L NAA-NA and 100-400 mg/L IBA-K + ;

Step e: Put the deepening tray into the simple culture room, control the temperature at 22-25°C, keep the humidity in the deepening tray at 100%, the light intensity 700～1200lx, and irrigate the cutting medium and its surrounding mixed substrate every 3 days 40ml of rooting solution was irrigated 3 times; after cutting for 10 days, disinfectant was sprayed once every other week until all roots were rooted.
The cutting propagation method of A. pentaphyllum according to claim 1, characterized in that, in step a, the branches are three-year-old seedlings of A. pentaphylla or the half-woody branches of the current year of the annual tissue culture potted seedlings Slightly or mid-lower branches.
The cutting propagation method of A. pentaphyllum according to claim 1, characterized in that, in step a, the branches are the tops of current half-woody branches of the one-year-old tissue culture potted seedlings of A. pentaphyllum.
The cutting propagation method of Acer palmatum according to claim 1, characterized in that the specific step of sterilizing the mixed substrate in step c is: use a 500-fold solution of 75% wettable chlorothalonil powder on the mixed substrate Spray and mix evenly to ensure that the mixed substrate is covered with plastic film for one week after pouring, and then spray and rinse with water for 2 to 3 times and then put it into the deepening tray.
The cutting propagation method of Acer palmatum according to claim 1, characterized in that the composition of the rooting solution in step d includes: 50 mg/L NAA-NA and 200 mg/L IBA-K + .
The method for cutting propagation of A. pentaphyllum according to claim 1, wherein the disinfectant in step e is a solution of 800 times of 75% wettable chlorothalonil powder.