WO2018182316A2 - Method for detecting web-based nucleic acid double-strand break position and electronic device using same - Google Patents

Abstract

The present application relates to a method for detecting a web-based nucleic acid double-strand break position and an electronic device using the same, and according to an embodiment of the present invention, there is provided a method for detecting a web-based nucleic acid double-strand break position comprising: a step of outputting a data input area for acquiring sequence data relating to a gene sequence to a web browser; a step of detecting a target position and a non-target position through the position detection process on the basis of the sequence data input through the web browser and generating output data; and a step of outputting information on the detected target position and information on the detected non-target position on the web browser on the basis of the output data.

Description

Web-based nucleic acid double strand cleavage position detection method and electronic device using the same

The present application relates to a web-based nucleic acid double-strand cleavage position detection method and an electronic device using the same, and more particularly, by applying a gene shearing technique to generate both strands in the genome, both strands at a position other than the desired position The present invention relates to a web-based nucleic acid double-strand cleavage position detection method and an electronic device using the same to detect the position where the two-strand cleavage occurs through a computer-based analysis method to verify whether the cleavage occurred.

A program that selectively analyzes genetic information around the two-strand cleavage site resulting from the application of gene shears in whole genome sequencing data. Typically, these programs use a specific operating system (mostly Linux) and a specific language (primarily Python and Bash Script) to copy the results you want to analyze to your computer and then use a string-based user interface through a terminal prompt. It is driven by the Command-line User Interface.

Accessibility is limited because conventional analysis programs can only be used through a string-based user interface under the Linux environment. In other words, 1) experimenters should build an environment where a specific computer language and other operating systems (especially Linux) are installed, except for Windows, which is a commonly used operating system. Even with such an environment, there is a problem of obtaining a manpower that can utilize it properly. In order to overcome this, there have been attempts to build this kind of analysis program on a web server to analyze data in a relatively convenient manner, but also 1) legally when the data to be analyzed is derived from a patient. There is a problem that can not upload data to an external server, 2) if the size of the data to be analyzed is large, it takes longer than the actual analysis time.

One object of the present application is to provide a web-based nucleic acid double-stranded position detection program that anyone can easily access the analysis tool through a web-based interface to perform the analysis quickly without prior knowledge.

Another object of the present application is to provide a web-based nucleic acid double-stranded position detection program that is also easily provided with a machine-compiled program for users who feel a string-based user interface more comfortable.

The problem to be solved by the present application is not limited to the above-described problem, and the tasks not mentioned will be clearly understood by those skilled in the art from the present specification and the accompanying drawings. .

According to an aspect of the present application, a step of outputting a data input region for obtaining sequence data about a gene sequence in a web browser; Detecting a target position and a non-target position through the position detection process based on the sequence data input through the web browser and generating output data based on a second language format; and the web browser based on the output data. A method of detecting a web-based nucleic acid double-stranded position may be provided, including outputting information on the detected target position and information on a non-target position.

According to another aspect of the present application, an output unit for outputting a data input region for obtaining nucleic acid data relating to a nucleotide sequence on the web browser; And performing the position detection operation based on the nucleic acid data received through a web browser to detect both strand cutting positions, generating output data relating to the both strand cutting positions, and based on the output data. A control unit for outputting information on the two-strand cleavage position on the, characterized in that a web-based nucleic acid double strand cleavage position detection system may be provided.

Means for solving the problems of the present application are not limited to the above-described solutions, and the solutions not mentioned will be clearly understood by those skilled in the art from the present specification and the accompanying drawings. Could be.

According to the present application, a web-based nucleic acid double-stranded position detection program that allows anyone to easily access an analysis tool through a web-based interface to perform an analysis without prior knowledge can be provided.

According to the present application, a program compiled in machine language for a user who feels more comfortable with a string-based user interface may also be provided with a web-based nucleic acid double-stranded position detection program.

The effects of the present application are not limited to the above-described effects, and effects that are not mentioned will be clearly understood by those skilled in the art from the present specification and the accompanying drawings.

1 is a block diagram illustrating a unit of a web nucleic acid detection system according to an embodiment of the present application.

2 is a view showing the physical configuration of the web nucleic acid detection system according to an embodiment of the present application.

3 is a diagram illustrating components of a server according to an embodiment of the present application.

4 is a diagram illustrating components of a local device according to an embodiment of the present application.

5 and 6 are block diagrams illustrating the operation of the double strand cutting position detection system according to an embodiment of the present application.

7 is a view showing the operation of the double strand cutting position detection system according to another embodiment of the present application.

8 is a flowchart illustrating a position detection operation according to an embodiment of the present application.

9 is a diagram showing a web-based nucleic acid double-strand detection system actually implemented according to an embodiment of the present application.

The position of the nucleotide of the nucleic acid predesigned to be cleaved by a restriction enzyme in the double strand cleavage position, and the non-target position is the position of the nucleotide of the nucleic acid flagged as being cleaved by a restriction enzyme among the detected both strand cleavage positions Web-based nucleic acid double-strand cleavage position detection method can be provided, characterized in that not.

The generating of the output data may include: aligning nucleic acids of the nucleic acid data based on a reference nucleic acid; and aligning the nucleic acids of the nucleic acid data to a reference nucleic acid. Web-based nucleic acid double-strand cleavage position detection method may be provided, characterized in that corresponding to and aligned by the nucleotides of the reference nucleic acid.

In addition, the step of generating the output data, web-based nucleic acid double-strand cleavage position detection method, characterized in that for detecting the position of both strands by detecting the position of the nucleotide vertically aligned nucleotides of the aligned nucleic acid Can be.

In addition, the form of the two-strand cleavage position comprises a smooth end and an overhang, the smooth end is in the form of a two-strand cleavage position in which both strands of the nucleic acid vertically cut, the overhang is one strand of both strands of the nucleic acid Web-based nucleic acid bilateral cleavage position detection methods, which are in the form of protruding cleaved bi-strand cleavage positions, can be provided.

In addition, the detection operation for detecting the smooth end and the detection operation for detecting the overhang may be different, a web-based nucleic acid bilateral cleavage position detection method may be provided.

In addition, the step of generating the output data, the web-based nucleic acid amount, characterized in that for detecting the double-stranded cleavage position by sequentially reading the nucleotides of the aligned nucleic acid to detect the smooth end, the double peak pattern Strand cut position detection methods may be provided.

In the generating of the output data, the nucleotides of the aligned nucleic acids are sequentially read to detect the overhang, and the cleavage score is calculated using Equation 1 below to detect both strand cleavage positions. A web based nucleic acid double strand cleavage position detection method may be provided.

(Equation 1)

Fi: Forward read sequence starting at position i

Ri: the number of reverse read sequences starting at position i

Di: sequencing depth at i position

G: size of overhang

According to another aspect of the present application, an output unit for outputting a data input region for obtaining nucleic acid data relating to a nucleotide sequence on the web browser; And performing the position detection operation based on the nucleic acid data received through a web browser to detect both strand cutting positions, generating output data relating to the both strand cutting positions, and based on the output data. And a control unit for outputting information on the two-strand cutting position on the screen.

Hereinafter, a web-based nucleic acid double-strand cleavage position detection method and a system using the same will be described.

The web-based nucleic acid double strand cleavage position detection method may be defined as a method of detecting a double strand cleavage position in a nucleic acid based on a web browser. Specifically, the web-based nucleic acid double strand cleavage position detection system 1 may be defined as a system 1 that performs or uses a method for detecting a nucleic acid double strand cleavage position formed by a restriction enzyme on the web.

The nucleic acid may comprise a plurality of nucleotides. The nucleic acid may comprise a plurality of nucleotides linked to each other.

The nucleic acid both strands may refer to two nucleotide sequences that are complementarily bound.

The nucleic acid double strand cleavage position may refer to a position of a nucleotide sequence in which both nucleotide sequences of complementary strands are cleaved. Specifically, both nucleic acid cleavage positions may refer to positions of nucleotides in which both nucleotide sequences are cleaved. The double stranded cleavage site may comprise a target position and a non-target position. The form of the bi-strand cleavage site may comprise a smooth end and an overhang.

The restriction enzyme may cleave both strands, thereby forming a double strand cleavage site. The restriction enzyme may be predesigned to cleave a specific position of both strands. In other words, the restriction enzyme may cleave a specific position of both strands, and both strand cleavage positions that may be formed by the restriction enzyme may be specified.

The target position may be defined as a nucleic acid double strand cleavage position corresponding to the nucleic acid double strand cleavage position of the restriction enzyme designed among the nucleic acid double strand cleavage positions formed in the nucleic acid. The non-target position may be defined as a nucleic acid bi-strand cleavage position, not a bi-strand cleavage position of the restriction enzyme, among the nucleic acid bi-strand cleavage positions formed in the nucleic acid.

The blunt end may be defined in the form of a double-stranded cleavage site in which both strands of the nucleic acid are vertically cut, and the overhang may be defined in the form of a double-stranded cleavage site in which one of both strands of the nucleic acid is protruded.

1 is a block diagram illustrating a unit of a web nucleic acid detection system 1 according to an embodiment of the present application.

Referring to FIG. 1, the web nucleic acid detection system 1 according to an embodiment of the present application may include an input unit 10, a detection unit 20, a conversion unit 30, and an output unit 40. have. However, the web nucleic acid detection system 1 may be implemented which is not limited to the unit shown in FIG. 1 and includes more or fewer units.

The input unit 10 may receive data related to a nucleic acid. The detection unit 20 may detect the cleavage position in the nucleic acid. The conversion unit 30 may convert a language format. The output unit 40 may output information related to the cleavage position in the nucleic acid.

Meanwhile, data related to the nucleic acid may be defined as nucleic acid data.

Each of the units described above may be implemented based on a predetermined language format. The units are implemented in a language of a predetermined language format and may exist in the form of data that can be used in an electronic device.

Hereinafter, the language and language format will be described in detail.

The above-described units according to an embodiment of the present application may be implemented in a predetermined language to perform an operation suitable for an implementation purpose. In other words, the language may implement units that perform operations according to the implementation purpose.

The language format may be defined as a type of language. The language format may include a first language format and a second language format.

The language format may include a low level language and a high level language. The lower level language may include machine language and assembly language. The high-level languages include Basic, C, C #, C ++, D, F #, Python, Ruby, Java, JavaScript, asm.js, Pascal, Prolog, Fortran, COBOL, Lisp, Perl, R Groovy, Scala Can include, occam, and Swift languages.

The language format can define the environment in which the units can operate. In other words, an environment in which the unit may operate may be determined according to the language format of the language implementing the units.

The language form defines an environment in which the units can operate, and the units can perform operations under the defined environment. For example, a unit implemented in a C / C # / C ++ language format may be run in a Linux environment, and a unit implemented in a JavaScript / asm.js language format may be run in a web browser environment.

Hereinafter, each unit will be described in detail.

The input unit 10 may receive data related to a nucleic acid provided from a user of the system 1.

To this end, the input unit 10 may implement an input area 11 that can receive data related to nucleic acids on a web browser. A user of the system 1 can provide nucleic acid data to the system 1 via an input region 11 implemented by the input unit 10. In other words, the system 1 may be provided with nucleic acid data from the user via the input region 11 implemented by the input unit 10. Through the input area 11 implemented by the input unit 10, a user may easily input data and change the input data.

The detection unit 20 may detect the cleavage position in the nucleic acid based on the data related to the nucleic acid. The detection unit 20 may perform a position detection operation to generate data related to the cleavage position in the nucleic acid. Data related to the cleavage position in the nucleic acid can be defined as output data.

The conversion unit 30 may convert the language format of the detection unit 20. In other words, the conversion unit 30 may convert an environment in which the detection unit 20 may operate. In detail, the conversion unit 30 may convert a format of a language for implementing the detection unit 20. Alternatively, the conversion unit 30 may convert the format of the language of the result data output by the detection unit 20 being operated. For example, the conversion unit 30 may be Emscripten. Emscripten is a compiler that converts a program developed in computer languages such as C, C ++, Rust, etc. into a subset of JavaScript called asm.js. The converted program is a user's web without the existence of a web server 100. Data can be read and analyzed directly in the browser, which, unlike traditional slow JavaScript-based programs, can be as fast as comparable programs running directly on the computer.

That is, the detection unit 20 and the conversion unit 30 may be implemented in different language formats. The detection unit 20 may be implemented in a first language format, and the conversion unit 30 may be implemented based on a second language format. For example, the detection unit 20 may be implemented in a C language or C ++ language, and the conversion unit 30 may be implemented in a JavaScript-based language. As a result, the operating environment of the detection unit 20 may be Linux.

As a result, the detection unit 20 and the conversion unit 30 are implemented in different language formats, so that the user simply detects both strand cleavage positions in the nucleic acid using the web-based nucleic acid double strand cleavage position detection system 1. You can do it. When the detection unit 20 and the conversion unit 30 are implemented in the same language format so that they can be driven in a web browser, the detection unit 20 cuts both strands due to the limited operation that the language format can implement. Only a simple operation for detecting the position can be performed. In contrast, when the detection unit 20 and the conversion unit 30 are implemented in different language formats, the detection unit 20 is implemented in a language format language that can be operated in a complex language and the conversion unit 30 may allow the detection unit 20 to operate on a web browser, thereby allowing a user to simply use the high performance double strand cut position detection system 1 on the web browser.

The output unit 40 may cause the cleavage position in the detected nucleic acid to be visually recognized by the user via a web browser. The output unit 40 may provide the user of the system 1 with information on the position of both strand cuts based on the output data. The output unit 40 may form an output area 41 on a web browser, and output information on both strand cutting positions on the output area 41.

Meanwhile, the conversion unit 30 and the detection unit 20 may be transferred from one physical configuration to another physical configuration. Specifically, the conversion unit 30 and the detection unit 20 stored in one device may be transmitted to another device. In this case, the transmission may be performed by transmitting data.

The units of the web nucleic acid detection system 1 described above can be implemented in any physical configuration. In more detail, the physical configuration may be an electronic device. In other words, the above-described units may be implemented in an electronic device and perform the above-described operations on the electronic device.

Hereinafter, the physical configuration of the web nucleic acid detection system 1 will be described.

2 is a view showing the physical configuration of the web nucleic acid detection system 1 according to an embodiment of the present application.

Referring to FIG. 2, the web nucleic acid detection system 1 according to an embodiment of the present application may include a server 100 and a local device 200.

The server 100 may communicate with a plurality of electronic devices. The server 100 may communicate with the local device 200.

The local device 200 may refer to various types of electronic devices available to a user. The local device 200 is not limited to the illustrated back, but may be any electronic device that can be connected to the server. The electronic device may include a computer, a laptop, a smartphone, a PDA, a smart band, a smart watch, and the like.

In this case, the above-described units may be implemented in the server 100 and the local device 200. For example, the detection unit 20 and the conversion unit 30 may be implemented in the server 100, but the input unit 10 and the output unit 40 may be implemented in the local device 200.

On the other hand, the web nucleic acid detection system 1 without being limited to the physical configuration shown in FIG. 2 may be implemented with the web nucleic acid detection system 1 having a smaller physical configuration. For example, the web nucleic acid detection system 1 may include only the local device 200. In this case, the above-described units are implemented only in the local device 200, and the above-described units operate in the local device 200 to perform a web nucleic acid detection method.

Hereinafter, the components included in the server 100 and the local device 200 will be described in detail.

3 is a diagram illustrating the components of the server 100 according to an embodiment of the present application.

4 is a diagram illustrating components of the local device 200 according to an embodiment of the present application.

Referring to FIG. 3, the server 100 may include a server communication unit 110, a server database 120, and a server controller 130. However, the present invention is not limited to FIG. 3, and a server 100 including more components may be implemented.

The server communication unit 110 may communicate with an external device (eg, the local device 200). The server 100 may transmit and receive information with an external device through the server communication unit 110. For example, the server 100 may transmit the detection unit 20 and the conversion unit 30 to the local device 200 connected to the server 100 through the web browser using the server communication unit 110.

Here, communication, that is, the transmission and reception of data may be made by wire or wireless. To this end, the communication unit is a wired communication module connected to the Internet through a local area network (LAN), a mobile communication module connected to a mobile communication network via a mobile communication base station, and a wireless local area network (WLAN) such as Wi-Fi. GNSS (Global Navigation Satellite System) such as near field communication module and Global Positioning System (GPS) using area network (LAN) communication method or WPAN (wireless personal area network) communication method such as Bluetooth and Zigbee ) May be configured as a satellite communication module or a combination thereof.

The server database 120 may store various kinds of information. The server database 120 may store data temporarily or semi-permanently. For example, the server database 120 of the server 100 may include an operating system (OS) for driving the server 100, a program or application for generating data or braille for hosting a web site (for example, For example, data relating to a web application) may be stored, and data related to the detection unit 20 and the conversion unit 30 may be stored.

Examples of the server database 120 include a hard disk drive (HDD), a solid state drive (SSD), a flash memory, a read-only memory (ROM), and a random access memory (RAM). And the like. Such a database may be provided in a built-in type or a removable type.

The server controller 130 controls the overall operation of the server 100. To this end, the controller may perform calculation and processing of various information and control the operation of the components of the server 100. For example, the server controller 130 may execute a program or an application for detecting the nucleic acid double strand cutting position. The server controller 130 may be implemented as a computer or a similar device according to hardware software or a combination thereof. In hardware, the server controller 130 may be provided in the form of an electronic circuit that processes an electrical signal to perform a control function. In software, the server controller 130 may be provided in the form of a program for driving the server controller 130 in hardware. . Meanwhile, in the following description, unless otherwise specified, the operation of the server 100 may be interpreted to be performed by the control of the server controller 130.

Referring to FIG. 4, the local device 200 may include a local input / output unit 210, a local communication unit 220, a local storage unit 230, and a local control unit 240. However, the local device 200 is not limited to FIG. 4 and includes more components.

The local communication unit 220, the local storage unit 230, or the local control unit 240 may be implemented similarly to the server communication unit 110, the server database 120, and the server controller 130 of the server 100 described above. .

The local input / output unit 210 may be various interfaces or connection ports for receiving user input or outputting information to the user. The local input / output unit 210 may be divided into an input module and an output module. The input module receives a user input from a user. The user input may be in various forms, including key input, touch input, and voice input. Examples of such input modules that can receive user input include a conventional keypad, keyboard, and mouse, as well as a touch sensor for detecting a user's touch, a microphone for receiving a voice signal, a camera for recognizing a gesture through image recognition, Proximity sensor consisting of illuminance sensor or infrared sensor that detects user's approach, motion sensor that recognizes user's motion through acceleration sensor, gyro sensor, etc. and various other input means for detecting or receiving various types of user input It is a comprehensive concept that includes all of them. The touch sensor may be implemented as a piezoelectric or capacitive touch sensor that senses a touch through a touch panel or a touch film attached to the display panel, an optical touch sensor that senses a touch by an optical method, and the like. In addition, the input module may be implemented in the form of an input interface (USB port, PS / 2 port, etc.) for connecting an external input device that receives user input instead of a device that detects user input. The output module can also output a variety of information and provide it to the user. The output module is a comprehensive concept that includes a display for outputting an image, a speaker for outputting sound, a haptic device for generating vibrations, and various other forms of output. In addition, the output module may be implemented in the form of a port type output interface connecting the individual output means described above.

For example, the output module in the form of a display may display text, still images, and moving images. Displays include Liquid Crystal Display (LCD), Light Emitting Diode (LED) Display, Organic Light Emitting Diode (OLED) Display, Flat Panel Display (FPD), Transparent Display display, curved display, flexible display, 3D display, holographic display, projector, and other types of devices capable of performing image output functions. It is a concept that means a wide image display device that includes all of them. Such a display may be in the form of a touch display integrated with a touch sensor of the input module.

Accordingly, the local device 200 may exchange data by accessing the server 100 through a web browser using the local communication unit 220. In addition, the local storage 230 of the local device 200 may store an operating program (OS) or a web browser for driving the local device 200, and in addition, generates data related to braille generation. A program or an application (for example, a web application running on a web browser) or data regarding braille generation may be stored. In addition, the local controller 240 may execute a web browser or an application for detecting a program or both strand cutting positions.

Hereinafter, the double strand cut position detection operation of the double strand cut position detection system 1 will be described.

5 and 6 are block diagrams illustrating the operation of the double strand cut position detection system 1 according to an embodiment of the present application.

Referring to FIG. 5, a two-strand cut position system 1 includes a local device 200 that includes an input unit 10 and an output unit 40, a server that includes a detection unit 20, and a conversion unit 30. 100 may be implemented. In this case, the detection unit 20 and the conversion unit 30 of the server 100 may be provided to the local device 200. In other words, the local device 200 may obtain the detection unit 20 and the conversion unit 30 from the server 100. In detail, when the local device 200 connects to the server 100 through a web browser, the detection unit 20 and the conversion unit 30 may be provided from the server 100.

Each unit of the local device 200 may exchange predetermined data. The local device 200 receives the first data FD1 through the input unit 10 and provides the first data FD1 to the detection object 50 to detect both strand cleavage positions in the nucleic acid. The data FD2 may be generated and the second data FD2 may be provided to the output unit 40. The detection object 50 will be described later in detail.

Alternatively, according to another embodiment, the detection object 50 may be implemented by the detection unit 20 and the conversion unit 30 implemented in the server 200. In this case, the local device 200 provides the first data FD1 to the server 200 through the input unit 10, and the detection object 50 included in the server 200 is the nucleic acid. The second strand FD2 may be generated and provided to the local device 200 by detecting a cutting position in the two strands.

The first data FD1 may be nucleic acid data, and the second data FD2 may be output data.

In this case, the language formats of the first data FD1 and the second data FD2 may be different. Alternatively, environments in which the first data FD1 and the second data FD2 may be used may be different. For example, the first data FD1 may be data that can be used in a Linux environment, and the second data FD2 may be data that can be used in a web browser.

Referring to FIG. 6, the two-strand cut position detection system 1 may perform a position detection operation.

The local device 200 of the double strand cutting position detection system 1 may obtain the detection unit 20 and the conversion unit 30 from the server 100 (S110). The detection unit 20 and the conversion unit 30 may be implemented as a detection object 50. The environment in which the detection unit 20 may be operated may be converted by the conversion unit 30 (S120). For example, when the detection unit 20 is implemented in a language format language that can be driven in a Linux environment, the conversion unit 30 is configured to allow the detection unit 20 to be driven in a web browser environment. The environment in which the detection unit 20 may be driven may be converted (S130). Accordingly, the detection object 50 that can be driven in a web browser environment may be generated. The input unit 10 of the local device 200 may transfer the nucleic acid data received through the input region 11 to the implemented detection object 50 (S140). The detection object 50 may perform a double strand cutting position detection operation (hereinafter, a position detection operation) (S150). Second data may be generated according to the position detection operation, and the second data may be provided to the output unit 40 (S160). The output unit 40 may allow information related to both strand cutting positions to be output through a web browser according to the output operation based on the output data (S170).

The above-described detection object 50 may function to perform a location detection operation. The detection object 50 may mean a virtual object capable of performing a position detection operation. The detection object 50 may be stored in the local storage 230 of the local device 200. The detection object 50 may be driven on a web browser. The detection object 50 may be used interchangeably with the term detection instance.

Meanwhile, not all of the above-described steps S110 to S170 need to be performed, but at least one or more of steps S110 to S170 may be performed. In addition, the above-described step S110 to step S170 may be performed in various orders without being limited to the above-described order.

7 is a view showing a position detection operation of the two-strand cut position detection system according to another embodiment.

Referring to FIG. 7, the double strand position break detection system 1 includes a detection unit 20 and a conversion unit 30. The detection unit 20 and the conversion unit 30 may be implemented as the detection object 50. The operating environment of the detection unit 2 can be converted by the conversion unit 30. (S181)

For example, when the detection unit 20 is implemented in a language format that can be driven in a Linux environment, the conversion unit 30 may be configured such that the detection unit 20 can be driven in a web browser environment. 20) can change the environment. (S182)

Accordingly, the detection object 50 that can be driven in the web browser environment may be generated.

The local device 200 may include an input unit 10 and an output unit 40. The input unit 10 may transfer the nucleic acid data received through the input region 11 to the implemented detection object 50. (S183)

The detection object 50 may perform a location detection operation. (S184)

Second data may be generated according to the position detection operation, and the second data may be provided to the output unit 40. (S185)

The output unit 40 may output information related to both strand cutting positions through a web browser according to the output operation based on the output data. (S186)

Hereinafter, the position detection operation of the above-described detection object 50 will be described in detail.

8 is a flowchart illustrating a position detection operation according to an embodiment of the present application.

Referring to FIG. 8, the position detection operation may include an alignment step S210, a detection step S220, a target position and a non-target position detection step S230. It is not always necessary to perform all of steps S210 to S230, and at least one of steps S210 to S230 may be performed. In addition, the above-described step S210 to step S230 may be performed in various orders without being limited to the above-described order.

In the sorting step S210, the detection object 50 may sort data related to a plurality of nucleic acids in the nucleic acid data. Specifically, the detection object 50 may sort the data related to the nucleic acid based on the reference nucleic acid data. Accordingly, the plurality of nucleic acid data in the nucleic acid data can be sorted based on the reference nucleic acid data. The alignment may mean mapping the nucleic acid data to the reference nucleic acid data and then arranging bases having the same position in each position.

In the detection step (S220), the detection object 50 may detect both strand cleavage positions in the aligned nucleic acid data. To this end, the detection object 50 may detect vertically aligned positions within the aligned nucleic acid data. Alternatively, the detection object 50 may detect a double peak pattern. Alternatively, the detection object 50 may sequentially read the aligned nucleic acid data and assign a score according to Equation 1 below to detect both strand cutting positions.

Fi: Forward read sequence starting at position i

Ri: the number of reverse read sequences starting at position i

Di: sequencing depth at i position

G: size of overhang, or constant

Through Equation 1, a cleavage score associated with the position of each nucleotide of the nucleic acid can be calculated. If the cleavage score of the position of the nucleotide of the nucleic acid is above a predetermined criterion, the nucleotide may be determined as the cleavage position. The criteria for the score can be appropriately adjusted by those skilled in the art according to the purpose.

The above-described methods that the detection object 50 can use to detect the double stranded cutting position can be implemented in combination with each other. Accordingly, the reliability of the double strand cutting position detected by the system 1 can be increased.

In the target position and non-target position detection step (S230), the detection object 50 may determine the type of the detected double strand cutting position. The detection object 50 may determine whether the detected cutting position is a target position or a non-target position based on the double-strand cutting position designed by the restriction enzyme. Specifically, when the detected double strand cutting position is a predesigned double strand cutting position, the detected double strand cutting position is determined as a target position, and when the detected double strand cutting position is not the designed double strand cutting position, Can be determined by non-target location,

9 is a diagram showing a web-based nucleic acid double strand detection system 1 which is actually implemented according to one embodiment of the present application.

Referring to FIG. 9, an input region 11 into which a nucleic acid data can be input on a web browser of a local device 200 is implemented, and the nucleic acid data is provided to the system 1 through the input region 11. Can be. The nucleic acid data provided to the system 1 may be generated as output data according to a position detection operation performed on a web browser. The output data may be output as information related to both strand cutting positions on the output area 41 formed on the web browser.

In the above-described web-based nucleic acid double strand detection method according to the present application and an electronic device using the same, the steps constituting each embodiment are not essential, and therefore, each embodiment may optionally include the above-described steps. In addition, each step constituting each embodiment is not necessarily to be performed in the order described, the steps described later may be performed before the steps described first. It is also possible that any one step is performed repeatedly while each step is in operation.

Claims (15)

1. Outputting a data input area for obtaining nucleic acid data relating to a nucleotide sequence on a web browser;

   Performing a position detection operation based on the nucleic acid data received through the web browser to detect both strand cutting positions, and generating output data regarding the both strand cutting positions; And

   And outputting information on the two-strand cutting position on the web browser based on the output data.

   Web-based nucleic acid double-strand cleavage position detection method.
2. According to claim 1,

   The detection data used for the detection operation includes first detection data and second detection data,

   Wherein the first detection data is data for detecting a double-strand cleavage position in the nucleic acid, and the second detection data is data for converting an environment in which the first detection data is used.

   Web-based nucleic acid double-strand cleavage position detection method.
3. The method of claim 2,

   The first detection data is implemented in a language used in a Linux environment, and the second detection data converts an environment used for the first detection data to generate a detection object that performs an operation on a web browser. ,

   Web-based nucleic acid double-strand cleavage position detection method.
4. The method of claim 3, wherein

   The output data generated according to the detection operation performed based on the first detection data and the nucleic acid data is implemented in a language of a language format output from the web browser by the second detection data,

   Web-based nucleic acid double-strand cleavage position detection method.
5. The method of claim 4, wherein

   The language format of the second detection data is a JavaScript-based language format.

   Web-based nucleic acid double-strand cleavage position detection method.
6. The method of claim 5,

   The generating of the output data may include detecting the both-strand cutting position based on a detection operation performed by the detection object on the web browser.

   Web-based nucleic acid double-strand cleavage position detection method.
7. The method of claim 6,

   Generating the output data, characterized in that for detecting the target position and the non-target position of the two-strand cleavage position in the nucleic acid,

   Web-based nucleic acid double-strand cleavage position detection method.
8. The method of claim 7, wherein

   The target position is the position of the nucleotide of the nucleic acid predesigned to be cleaved by a restriction enzyme among the detected double strand cleavage positions,

   Wherein said non-target position is not the position of a nucleotide of said nucleic acid that is flagged as being cleaved by a restriction enzyme in said detected double strand cleavage position,

   Web-based nucleic acid double-strand cleavage position detection method.
9. The method of claim 8,

   The generating of the output data includes: sorting nucleic acids of the nucleic acid data based on a reference nucleic acid; and

   The aligning may be performed by mapping a nucleic acid of the nucleic acid data to a reference nucleic acid and aligning the nucleotides of the nucleic acid with the nucleotides of the reference nucleic acid.

   Web-based nucleic acid double-strand cleavage position detection method.
10. The method of claim 9,

    The step of generating the output data, characterized in that for detecting the position of both strands cleavage by detecting the position of the nucleotide vertically aligned both strands of the aligned nucleic acid,

    Web-based nucleic acid double-strand cleavage position detection method.
11. The method of claim 10,

    The form of both strand cleavage sites comprises a smooth end and an overhang,

    The smooth end is in the form of a double-stranded cleavage position in which both strands of the nucleic acid is vertically cut, the overhang is in the form of a double-stranded cleavage position in which one of both strands of the nucleic acid is protruded,

    Web-based nucleic acid double-strand cleavage position detection method.
12. The method of claim 11, wherein

    The detection operation for detecting the smooth end and the detection operation for detecting the overhang are different from each other.

    Web-based nucleic acid double-strand cleavage position detection method.
13. The method of claim 12,

    The generating of the output data may include detecting the double-stranded cleavage position by sequentially reading nucleotides of the aligned nucleic acids to detect the blunt ends, and detecting a double peak pattern.

    Web-based nucleic acid double-strand cleavage position detection method.
14. The method of claim 9,

    In the generating of the output data, the nucleotides of the aligned nucleic acids are sequentially read in order to detect the overhang, and the truncation score is calculated using Equation 1 below to detect both strand cleavage positions.

    Web-based nucleic acid double-strand cleavage position detection method.

    (Equation 1)

    

    Fi: Forward read sequence starting at position i

    Ri: the number of reverse read sequences starting at position i

    Di: sequencing depth at i position

    G: size of overhang
15. An output unit for outputting a data input region for acquiring nucleic acid data relating to a nucleotide sequence on the web browser; And

    The position detection operation is performed on the basis of the nucleic acid data received through the web browser to detect both strand cutting positions, and generates output data relating to the both strand cutting positions, and the web browser is based on the output data. And a control unit for outputting information on the cutting position of both strands on the screen.

    Electronics.

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