WO2001079445A3 - Pluripotent cells comprising allogenic nucleus and mitochondria - Google Patents

Pluripotent cells comprising allogenic nucleus and mitochondria Download PDF

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Publication number
WO2001079445A3
WO2001079445A3 PCT/US2001/012265 US0112265W WO0179445A3 WO 2001079445 A3 WO2001079445 A3 WO 2001079445A3 US 0112265 W US0112265 W US 0112265W WO 0179445 A3 WO0179445 A3 WO 0179445A3
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WO
WIPO (PCT)
Prior art keywords
mitochondria
pluripotent cells
cells
allogenic
nucleus
Prior art date
Application number
PCT/US2001/012265
Other languages
French (fr)
Other versions
WO2001079445A2 (en
Inventor
Michael D West
Original Assignee
Advanced Cell Tech Inc
Michael D West
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Tech Inc, Michael D West filed Critical Advanced Cell Tech Inc
Priority to IL15206401A priority Critical patent/IL152064A0/en
Priority to NZ521711A priority patent/NZ521711A/en
Priority to AU2001257053A priority patent/AU2001257053A1/en
Priority to JP2001577429A priority patent/JP2003530848A/en
Priority to BR0110072-6A priority patent/BR0110072A/en
Priority to EP01930525A priority patent/EP1282685A2/en
Priority to CA002405555A priority patent/CA2405555A1/en
Priority to MXPA02010075A priority patent/MXPA02010075A/en
Publication of WO2001079445A2 publication Critical patent/WO2001079445A2/en
Publication of WO2001079445A3 publication Critical patent/WO2001079445A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/166—Animal cells resulting from interspecies fusion
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Allochimeric ES cells are provided having nuclei from one individual of a species and mitochondria from a different individual of the same species. The ES cells can be used for differentiation into differentiated cell types for therapy or for studying various developmental processes in forming embryos and differentiation.
PCT/US2001/012265 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria WO2001079445A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
IL15206401A IL152064A0 (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria
NZ521711A NZ521711A (en) 2000-04-14 2001-04-16 Modified oocytes and embryonic non-human stem cells able to be cultured into a variety of differentiated cell types
AU2001257053A AU2001257053A1 (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria
JP2001577429A JP2003530848A (en) 2000-04-14 2001-04-16 Pluripotent cells containing allogeneic nuclei and mitochondria
BR0110072-6A BR0110072A (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogeneic nucleus and mitochondria
EP01930525A EP1282685A2 (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria
CA002405555A CA2405555A1 (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria
MXPA02010075A MXPA02010075A (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19740700P 2000-04-14 2000-04-14
US60/197,407 2000-04-14

Publications (2)

Publication Number Publication Date
WO2001079445A2 WO2001079445A2 (en) 2001-10-25
WO2001079445A3 true WO2001079445A3 (en) 2002-07-11

Family

ID=22729292

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/012265 WO2001079445A2 (en) 2000-04-14 2001-04-16 Pluripotent cells comprising allogenic nucleus and mitochondria

Country Status (10)

Country Link
EP (1) EP1282685A2 (en)
JP (1) JP2003530848A (en)
CN (2) CN1427887A (en)
AU (1) AU2001257053A1 (en)
BR (1) BR0110072A (en)
CA (1) CA2405555A1 (en)
IL (1) IL152064A0 (en)
MX (1) MXPA02010075A (en)
NZ (1) NZ521711A (en)
WO (1) WO2001079445A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1373474A2 (en) 2001-01-02 2004-01-02 Stemron, Inc. A method for producing a population of homozygous stem cells having a pre-selected immunophenotype and/or genotype
WO2003072708A2 (en) * 2002-02-21 2003-09-04 Advanced Cell Technology, Inc. Pluripotent cells comprising allogenic nucleus and mitochondria
AU2012242591B2 (en) * 2011-04-14 2014-10-23 The General Hospital Corporation Compositions and methods for autologous germline mitochondrial energy transfer
CN104830777A (en) * 2014-02-28 2015-08-12 复旦大学 Polar body genome reconstruction ovum, preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EVANS M.J. ET AL.: "Mitochondrial DNA genotypes in nuclear transfer-derived sheep", NATURE GENETICS, vol. 23, September 1999 (1999-09-01), pages 90 - 93, XP002946451 *
TAKEDA K. ET AL.: "Dominant distribution of mitochondrial DNA from recipient oocytes in bovine embryos and offspring after nuclear transfer", JOURNAL OF REPRODUCTION AND FERTILITY, vol. 116, 1999, pages 253 - 259, XP002946449 *
WOLF D.P. ET AL.: "Nuclear transfer in the rhesus monkey: Practical and basic implications", BIOLOGY OF REPRODUCTION, vol. 60, 1999, pages 199 - 204, XP002946450 *

Also Published As

Publication number Publication date
CN1673361A (en) 2005-09-28
MXPA02010075A (en) 2003-02-12
WO2001079445A2 (en) 2001-10-25
CN1427887A (en) 2003-07-02
CA2405555A1 (en) 2001-10-25
BR0110072A (en) 2004-07-06
EP1282685A2 (en) 2003-02-12
AU2001257053A1 (en) 2001-10-30
NZ521711A (en) 2005-03-24
IL152064A0 (en) 2003-05-29
JP2003530848A (en) 2003-10-21

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