US3578291A - Test tube having integral mixing means - Google Patents

Test tube having integral mixing means Download PDF

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Publication number
US3578291A
US3578291A US861251A US3578291DA US3578291A US 3578291 A US3578291 A US 3578291A US 861251 A US861251 A US 861251A US 3578291D A US3578291D A US 3578291DA US 3578291 A US3578291 A US 3578291A
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test tube
longitudinal axis
sidewall
stirring
tube
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US861251A
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Rudolf Oberli
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Greiner Electronic AG
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Greiner Electronic AG
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions

Definitions

  • Laubscher ABSTRACT A test tube for mixing liquids, characterized by the provision of integral stirring means extending longitudifially from the bottom wall in spaced relation to the sidewall of the tube.
  • the stirring means preferably is of knifelike configuration including a longitudinal edge adjacent and spaced from the internal surface of the sidewall whereby upon oscillation of the test tube about its longitudinal axis, a vortex is formed adjacent the edge to effect rapid thorough mixing.
  • the test tube has a capacity of between about 0.5 and 5 milliliters and is designed for disposal after a single use.
  • test tubes which usually have a capacity of between approximately 0.5 and milliliters.
  • the test tubes themselves are intended generally to be used only once, and must therefore be relatively inexpensive.
  • the present invention relates to a test tube having a capacity within the milliliter range which is designed preferably for being used only once and consists of an essentially cylindrical tube having an integral bottom wall.
  • at least one integral knifelike stirring means is disposed coaxially within the test tube of the present invention, which stirring means extend radially outwardly from the longitudinal axis of the tube to define a gap between its outer pointed extremity and the inner surface of the test tube wall.
  • the stirring means are integral with the bottom of the test tube and extend upwardly in spaced relation to the test tube sidewall.
  • the stirring means has a double-knife configuration including a pair of pointed portions that extend diametrically radially outwardly from the longitudinal axis of the test tube.
  • the inner wall surface of the tube is smooth throughout, whereas the outer extremities of the pointed projections are provided with sharp edges that extend longitudinally adjacent and spaced from the wall surface.
  • the sidewall surfaces of the stirring means are also smooth.
  • FIG. 1 is a longitudinal cross section view of the improved mixing test tubule of the present invention.
  • FIG. 2 is a sectional view taken along line 2-2 of FIG. 1, illustrating diagrammatically the means for supporting and oscillating the test tube.
  • the test tube according to FlGS. 1 and 2 is made preferably of an inexpensive impervious material, such as polypropylene for example, and includes a tubular sidewall portion 3 and an integral bottom wall portion 4.
  • the upper surface of the bottom wall is planar and is arranged at a right angle with respect to the longitudinal axis 5.
  • double knife edge stirring means including symmetrical pointed portions 6, 6 that extend diametrically radially outwardly from the longitudinal axis 5 and terminate in longitudinal edges 7, 7' that are adjacent and spaced form the inner wall.
  • the stirring means is integral with and extends upwardly from the bottom wall 4 in concentrically spaced relation to the tubular wall 3.
  • the inner wall surface of the tube 3, the upper surface of the bottom 4 and the lateral surfaces of the stirring means 6, 6 all have completely smooth surfaces.
  • the tube 3 is provided with guide pins 9 and 9' and 10, 10 that project laterally outwardly from the tube for reception in the tube holder means that are driven by conventional oscillatory drive means as shown in FIG. 2.
  • the test tube illustrated in the drawing is intended for a capacity within the milliliter range, the height of the tube 3 being on the order of 5.0 centimeters, for a tube having an inside diameter of approximately l.3 centimeters.
  • test tube is axially inserted or slid downwardly into the supporting means within which it is held securely against torsion by means of the guide pins 9, 9 and 10, 10.
  • the holder is oscillated about its longitudinal axis 5 by the oscillatory drive means. ln the case of a test tube having the dimensions set forth above, it has been found advantageous to employ an amplitude of the rotary oscillations of approximately :40 radians if the frequency of the rotary oscillations is between 5 and 10 cycles per second.
  • test tube When a liquid to be mixed is inserted into the test tube and sinusoidal rotational oscillations are imparted to the tube, considerable relative velocities of the liquid with respect to the tubular wall are temporarily produced in the gaps 8 and 8'. A vigorous vortex formation is produced adjacent the sharp edges 7 and 7 which, in turn, brings about a rapid and thorough stim'ng or intermixture of the liquid. It is a particular advantage of the present invention that there will be no formation of bubbles and no spilling of the liquid.
  • the test tube may therefore be filled to a point near the upper rounded-off portion of the tongues'6 and 6' (FIG. 1).
  • the desired stirring or intimate mixing effect will be produced also if the test tubule is filled only for a very small fraction of the tubular height thereof.
  • test tube adapted for mixing a liquid and including integral bottom 4 and tubular sidewall 3'portions defining a cavity within the milliliter range
  • said test tube includes at least one stirring portion 6, 6' arranged in coaxially spaced relation within said tubular portion and integrally connected at one end with said bottom portion, said stirring portion having a knifelike configuration including in transverse cross section at least one radially outwardly extending pointed portion defining a longitudinal edge 7, 7 adjacent and spaced form the inner wall surface of said tubular portion, whereby when said test tube is oscillated about its longitudinal axis, a vortex is formed in the gap 8, 8' adjacent the edge to effect rapid mixing of the liquid in the test tube.
  • test tube as defined in claim 1, wherein the inner surface of said cylindrical sidewall portion is smooth.
  • test tube as defined in claim 1, wherein the surface carried by said bottom wall defining the bottom of said cavity is planar and arranged at right angles to the longitudinal axis of said test tube.
  • Apparatus defined in clairn 6 wherein the length and the internal diameter of the tubular sidewall portion are about 5.0 centimeters and 1.3 centimeters, respectively.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

A test tube for mixing liquids, characterized by the provision of integral stirring means extending longitudinally from the bottom wall in spaced relation to the sidewall of the tube. The stirring means preferably is of knifelike configuration including a longitudinal edge adjacent and spaced from the internal surface of the sidewall whereby upon oscillation of the test tube about its longitudinal axis, a vortex is formed adjacent the edge to effect rapid thorough mixing. Preferably the test tube has a capacity of between about 0.5 and 5 milliliters and is designed for disposal after a single use.

Description

United States Patent 7 Claims, 2 Drawing Figs.
US. Cl 259/75,
1nt.Cl B011 11/00 Field of Search 259/56, 75,
76, 80, (Digest), 42; 68/154, 174
I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I i I [56] References Cited UNITED STATES PATENTS 1,298,832 4/1919 Tracy 68/154 2,965,274 12/1960 Brillis et al 259/76X 3,432,149 3/1969 Stalberg et a1. 259/75 FOREIGN PATENTS 757,061 9/1956 Great Britain 68/174 Primary Examiner-Walter A. Scheel Assistant ExaminerAlan l. Cantor Attorney-Lawrence E. Laubscher ABSTRACT: A test tube for mixing liquids, characterized by the provision of integral stirring means extending longitudifially from the bottom wall in spaced relation to the sidewall of the tube. The stirring means preferably is of knifelike configuration including a longitudinal edge adjacent and spaced from the internal surface of the sidewall whereby upon oscillation of the test tube about its longitudinal axis, a vortex is formed adjacent the edge to effect rapid thorough mixing. Preferably the test tube has a capacity of between about 0.5 and 5 milliliters and is designed for disposal after a single use.
Patehted 1 May 11, 1971'- 3,578,291
I oscillatory Drive Means 1 NVEN TOR. Rudolf Oberl/ BY g I 1 ATTORNE? TEST TUBE HAVING INTEGRAL MIXING MEANS When performing chemical and/or physical analyses, it is frequently necessary to thoroughly mix or to stir the samples present in a container. Stirring or mixing blades or paddles are effect of the stirring paddles, it has been proposed to provide radially inwardly directed baffles adjacent the inner wall of the container. In this case, the container may also be subjected to rotary oscillations which have an opposite direction relative to the rotary oscillations of the stirring paddles. The containers suitable therefor have a capacity of from several liters to a few deciliters, and in order to avoid spillage during the mixing operation, the containers can be filled to only a fraction of their actual capacity.
For numerous investigations, however, only limited quantities of the samples are available and are therefore introduced into test tubes which usually have a capacity of between approximately 0.5 and milliliters. The test tubes themselves are intended generally to be used only once, and must therefore be relatively inexpensive. These and other requirements arising in the case of test tubes prevent the use of the prior mixing means that are customarily employed in larger containers for the purpose of stirring or thoroughly mixing the samples, and in view of the relatively small capacity of the test tubes, the use of stirring paddles which are moved relative .to the inner wall of the test tubes is out of the question.
Accordingly, the present invention relates to a test tube having a capacity within the milliliter range which is designed preferably for being used only once and consists of an essentially cylindrical tube having an integral bottom wall. In accordance with the primary object of the invention, in order to obtain thorough mixing of the contents of the test tube by merely subjecting the test tube as a whole to oscillatory movement about the longitudinal axis thereof, at least one integral knifelike stirring means is disposed coaxially within the test tube of the present invention, which stirring means extend radially outwardly from the longitudinal axis of the tube to define a gap between its outer pointed extremity and the inner surface of the test tube wall. The stirring means are integral with the bottom of the test tube and extend upwardly in spaced relation to the test tube sidewall. According to a preferred embodiment of the invention, the stirring means has a double-knife configuration including a pair of pointed portions that extend diametrically radially outwardly from the longitudinal axis of the test tube. Preferably the inner wall surface of the tube is smooth throughout, whereas the outer extremities of the pointed projections are provided with sharp edges that extend longitudinally adjacent and spaced from the wall surface. Preferably, the sidewall surfaces of the stirring means are also smooth.
Other objects and advantages of the invention will become apparent from a study of the following specification, when viewed in the light of the accompanying drawing, in which;
FIG. 1 is a longitudinal cross section view of the improved mixing test tubule of the present invention; and
FIG. 2 is a sectional view taken along line 2-2 of FIG. 1, illustrating diagrammatically the means for supporting and oscillating the test tube.
The test tube according to FlGS. 1 and 2 is made preferably of an inexpensive impervious material, such as polypropylene for example, and includes a tubular sidewall portion 3 and an integral bottom wall portion 4. The upper surface of the bottom wall is planar and is arranged at a right angle with respect to the longitudinal axis 5. Disposed within the tube 3 are double knife edge stirring means including symmetrical pointed portions 6, 6 that extend diametrically radially outwardly from the longitudinal axis 5 and terminate in longitudinal edges 7, 7' that are adjacent and spaced form the inner wall.
surface to define narrow gaps 8, 8'. The stirring means is integral with and extends upwardly from the bottom wall 4 in concentrically spaced relation to the tubular wall 3. Preferably, the inner wall surface of the tube 3, the upper surface of the bottom 4 and the lateral surfaces of the stirring means 6, 6 all have completely smooth surfaces. Finally, the tube 3 is provided with guide pins 9 and 9' and 10, 10 that project laterally outwardly from the tube for reception in the tube holder means that are driven by conventional oscillatory drive means as shown in FIG. 2. The test tube illustrated in the drawing is intended for a capacity within the milliliter range, the height of the tube 3 being on the order of 5.0 centimeters, for a tube having an inside diameter of approximately l.3 centimeters.
During actual use, the test tube is axially inserted or slid downwardly into the supporting means within which it is held securely against torsion by means of the guide pins 9, 9 and 10, 10. The holder is oscillated about its longitudinal axis 5 by the oscillatory drive means. ln the case of a test tube having the dimensions set forth above, it has been found advantageous to employ an amplitude of the rotary oscillations of approximately :40 radians if the frequency of the rotary oscillations is between 5 and 10 cycles per second.
When a liquid to be mixed is inserted into the test tube and sinusoidal rotational oscillations are imparted to the tube, considerable relative velocities of the liquid with respect to the tubular wall are temporarily produced in the gaps 8 and 8'. A vigorous vortex formation is produced adjacent the sharp edges 7 and 7 which, in turn, brings about a rapid and thorough stim'ng or intermixture of the liquid. It is a particular advantage of the present invention that there will be no formation of bubbles and no spilling of the liquid. The test tube may therefore be filled to a point near the upper rounded-off portion of the tongues'6 and 6' (FIG. 1). On the other hand, the desired stirring or intimate mixing effect will be produced also if the test tubule is filled only for a very small fraction of the tubular height thereof.
While in accordance with the provisions of the Patent Statutes l have illustrated the preferred form and embodiment of the invention now known to me, it will be apparent to those skilled in the art that various modifications may be made in the disclosed apparatus without deviating from the inventive concept.
lclaim:
1. in a test tube adapted for mixing a liquid and including integral bottom 4 and tubular sidewall 3'portions defining a cavity within the milliliter range, the improvement wherein said test tube includes at least one stirring portion 6, 6' arranged in coaxially spaced relation within said tubular portion and integrally connected at one end with said bottom portion, said stirring portion having a knifelike configuration including in transverse cross section at least one radially outwardly extending pointed portion defining a longitudinal edge 7, 7 adjacent and spaced form the inner wall surface of said tubular portion, whereby when said test tube is oscillated about its longitudinal axis, a vortex is formed in the gap 8, 8' adjacent the edge to effect rapid mixing of the liquid in the test tube.
2. A test tube as defined in claim 1, wherein the inner surface of said cylindrical sidewall portion is smooth.
3. A test tube as defined in claim 2, wherein the opposed sidewall surfaces of said stirring portion are smooth.
f. A test tube as defined in claim 1, wherein the surface carried by said bottom wall defining the bottom of said cavity is planar and arranged at right angles to the longitudinal axis of said test tube. i
5. A test tube as defined in claim 1,'wfiiin' the stirring- 7. Apparatus defined in clairn 6, wherein the length and the internal diameter of the tubular sidewall portion are about 5.0 centimeters and 1.3 centimeters, respectively.

Claims (7)

1. In a test tube adapted for mixing a liquid and including integral bottom 4 and tubular sidEwall 3 portions defining a cavity within the milliliter range, the improvement wherein said test tube includes at least one stirring portion 6, 6'' arranged in coaxially spaced relation within said tubular portion and integrally connected at one end with said bottom portion, said stirring portion having a knifelike configuration including in transverse cross section at least one radially outwardly extending pointed portion defining a longitudinal edge 7, 7'' adjacent and spaced form the inner wall surface of said tubular portion, whereby when said test tube is oscillated about its longitudinal axis, a vortex is formed in the gap 8, 8'' adjacent the edge to effect rapid mixing of the liquid in the test tube.
2. A test tube as defined in claim 1, wherein the inner surface of said cylindrical sidewall portion is smooth.
3. A test tube as defined in claim 2, wherein the opposed sidewall surfaces of said stirring portion are smooth.
4. A test tube as defined in claim 1, wherein the surface carried by said bottom wall defining the bottom of said cavity is planar and arranged at right angles to the longitudinal axis of said test tube.
5. A test tube as defined in claim 1, wherein the stirring means has a double knife edge configuration including a pair of symmetrical pointed portions extending diametrically radially outwardly from the longitudinal axis of the test tube to define longitudinal edges adjacent and spaced form the inner surface of said sidewall portion, respectively.
6. A test tube as defined in claim 1, wherein the test tube has a capacity of between 0.5 and 5 milliliters, and further including means for oscillating said tube about its longitudinal axis through an angle of about + or - 40 radians at a frequency of oscillation of from 5 to 10 cycles per second.
7. Apparatus as defined in claim 6, wherein the length and the internal diameter of the tubular sidewall portion are about 5.0 centimeters and 1.3 centimeters, respectively.
US861251A 1969-01-22 1969-09-26 Test tube having integral mixing means Expired - Lifetime US3578291A (en)

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CH87669A CH475793A (en) 1969-01-22 1969-01-22 Sample tube

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US (1) US3578291A (en)
JP (1) JPS4941588B1 (en)
CH (1) CH475793A (en)
DE (2) DE1932368C3 (en)
FR (1) FR2028835A1 (en)
GB (1) GB1272828A (en)
SE (1) SE345207B (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5549574A (en) * 1994-08-30 1996-08-27 Eli Lilly And Company Cartridge assembly for use in a pen-type medicament injector
US5609822A (en) * 1995-07-07 1997-03-11 Ciba Corning Diagnostics Corp. Reagent handling system and reagent pack for use therein
EP0770207A1 (en) * 1994-07-12 1997-05-02 Brian S. Bull Rapid determination of blood sedimentation rate
US5795784A (en) * 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) * 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US6063340A (en) * 1991-03-04 2000-05-16 Chiron Diagnostics Corporation Reagent container for automated analyzer
US6066300A (en) * 1995-07-07 2000-05-23 Bayer Corporation Reagent handling system and configurable vial carrier for use therein
US6436349B1 (en) * 1991-03-04 2002-08-20 Bayer Corporation Fluid handling apparatus for an automated analyzer
US6498037B1 (en) * 1991-03-04 2002-12-24 Bayer Corporation Method of handling reagents in a random access protocol
US20060013729A1 (en) * 1991-02-14 2006-01-19 Glen Carey Fluid handling apparatus for an automated analyzer
US20080192567A1 (en) * 2007-02-08 2008-08-14 Kathleen Vincent Reagent cartridge mixing tube
US20090311775A1 (en) * 2005-12-22 2009-12-17 Sartorius Stedim Biotech Gmbh Disposable Bioreactor for Culturing Cells in a Nutrient Medium
CN113049758A (en) * 2021-04-07 2021-06-29 浙江衡谱医药有限公司 Detection device is used in drug intermediate research and development with contrast function

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0330085Y2 (en) * 1985-09-06 1991-06-26
DE4419971A1 (en) * 1994-06-08 1995-12-14 Eppendorf Geraetebau Netheler Polypropylene vessel for heating small quantities of liq.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1298832A (en) * 1914-03-12 1919-04-01 James J Tracy Apparatus for dyeing and the like.
GB757061A (en) * 1953-09-26 1956-09-12 Bosch Gmbh Robert Improvements in or relating to washing machines
US2965274A (en) * 1958-08-13 1960-12-20 Michael N Brillis Paper coffee cup
US3432149A (en) * 1966-04-27 1969-03-11 Nils Gustav Stalberg Apparatus for stirring a liquid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1298832A (en) * 1914-03-12 1919-04-01 James J Tracy Apparatus for dyeing and the like.
GB757061A (en) * 1953-09-26 1956-09-12 Bosch Gmbh Robert Improvements in or relating to washing machines
US2965274A (en) * 1958-08-13 1960-12-20 Michael N Brillis Paper coffee cup
US3432149A (en) * 1966-04-27 1969-03-11 Nils Gustav Stalberg Apparatus for stirring a liquid

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060013729A1 (en) * 1991-02-14 2006-01-19 Glen Carey Fluid handling apparatus for an automated analyzer
US6074615A (en) * 1991-03-04 2000-06-13 Bayer Corporation Reagent container for an automated analyzer
US7182912B2 (en) 1991-03-04 2007-02-27 Bayer Corporation Fluid handling apparatus for an automated analyzer
US20050266570A1 (en) * 1991-03-04 2005-12-01 Bayer Corporation Cuvette for an automated analyzer
US6555062B1 (en) 1991-03-04 2003-04-29 Bayer Corporation Reagent container for an automated analyzer
US6498037B1 (en) * 1991-03-04 2002-12-24 Bayer Corporation Method of handling reagents in a random access protocol
US6436349B1 (en) * 1991-03-04 2002-08-20 Bayer Corporation Fluid handling apparatus for an automated analyzer
US6063340A (en) * 1991-03-04 2000-05-16 Chiron Diagnostics Corporation Reagent container for automated analyzer
EP0770207A4 (en) * 1994-07-12 1999-10-13 Brian S Bull Rapid determination of blood sedimentation rate
EP0770207A1 (en) * 1994-07-12 1997-05-02 Brian S. Bull Rapid determination of blood sedimentation rate
US5549574A (en) * 1994-08-30 1996-08-27 Eli Lilly And Company Cartridge assembly for use in a pen-type medicament injector
US5788928A (en) * 1995-07-07 1998-08-04 Chiron Diagnostics Corporation Reagent handling system and reagent pack for use therein
US5609822A (en) * 1995-07-07 1997-03-11 Ciba Corning Diagnostics Corp. Reagent handling system and reagent pack for use therein
US6066300A (en) * 1995-07-07 2000-05-23 Bayer Corporation Reagent handling system and configurable vial carrier for use therein
US5856194A (en) * 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US5795784A (en) * 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US6562298B1 (en) 1996-09-19 2003-05-13 Abbott Laboratories Structure for determination of item of interest in a sample
US9174181B2 (en) 2005-12-22 2015-11-03 Sartorius Stedim Biotech Gmbh Disposable bioreactor for culturing cells in a nutrient medium
US20090311775A1 (en) * 2005-12-22 2009-12-17 Sartorius Stedim Biotech Gmbh Disposable Bioreactor for Culturing Cells in a Nutrient Medium
US20080192567A1 (en) * 2007-02-08 2008-08-14 Kathleen Vincent Reagent cartridge mixing tube
US20100187253A1 (en) * 2007-02-08 2010-07-29 Kathleen Vincent Reagent cartridge mixing tube
US8550697B2 (en) 2007-02-08 2013-10-08 Biokit, S.A. Reagent cartridge mixing tube
US7731414B2 (en) * 2007-02-08 2010-06-08 Instrumentation Laboratory Company Reagent cartridge mixing tube
US9636647B2 (en) 2007-02-08 2017-05-02 Biokit, S.A. Reagent cartridge mixing tube method
CN113049758A (en) * 2021-04-07 2021-06-29 浙江衡谱医药有限公司 Detection device is used in drug intermediate research and development with contrast function

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Publication number Publication date
GB1272828A (en) 1972-05-03
DE7000617U (en) 1970-09-17
SE345207B (en) 1972-05-23
DE1932368B2 (en) 1978-06-29
JPS4941588B1 (en) 1974-11-09
FR2028835A1 (en) 1970-10-16
CH475793A (en) 1969-07-31
DE1932368A1 (en) 1970-07-30
DE1932368C3 (en) 1979-02-22

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