TWI731244B - A composition containing a rutaceae plant extract - Google Patents

Abstract

The present invention provides a composition containing a Rutaceae plant extract, comprising a Rutaceae plant extract with an amount of 0.1-2 wt%, a C1-C4 polyol system with an amount of 10-30 wt% and 60-70 wt% pure water. The composition containing a Rutaceae plant extract of the present invention can be antibacterial and anti-viral in a short time, and has lubricating and moisturizing effects, moreover it is hygienic, safe and good in use.

Description

Compositions containing extracts from plants of the Rutaceae family

The present invention relates to a composition containing extracts from plants of the Rutaceae family.

Natural acidic lubricating fluid normally exists in the vagina, and the lubricating fluid will increase during sex. For some reasons, including reduction of estrogen during menopause, or after ovarian removal surgery, and radiation therapy, the lubricating fluid may decrease, causing vaginal tissues to become dry and irritated. This may cause pain and/or bleeding during sex. Oral contraceptives and certain drugs such as antihistamines, antidepressants, blood pressure lowering drugs, and heart drugs can also cause vaginal dryness. In addition, psychological conditions, including stress, fatigue, and anxiety, may hinder the production of natural lubricants. After childbirth, especially when the mother is breastfeeding, the combination of hormones and psychological factors may cause temporary vaginal dryness.

Common human body lubricants or skin care products and cleaning products, such as silicone and commercially available K-Y glue and Vaseline are mainly based on synthetic substances such as silicone and petrochemical raw materials. Therefore, such commercially available acid vaginal lubricants are not recommended for women who want to become pregnant or those who value natural, organic, preventive health care.

One object of the present invention is to provide a composition containing extracts of Rutaceae plants, which not only has maintenance effects such as lubrication and moisturizing, but also has the effects of short-term antibacterial, antiviral, and disease prevention.

In order to achieve the above objective, the present invention relates to a composition containing Rutaceae plant extracts, including 0.1-2wt% Rutaceae plant extracts, 10-30wt% C1-C4 polyol system and 60-70wt% pure water. In one aspect, the composition containing Rutaceae plant extracts of the present invention includes 0.1-2wt% Rutaceae plant extracts, 2-10wt% C5-C8 polyol system and 60-70wt% pure water. In one aspect, the Rutaceae plant extract is selected from tangerine, tangerine, An extract of any group consisting of sweet orange, lime, bitter orange, grapefruit, grapefruit, lemon, lime, citron, bergamot and kumquat. The polyol system is a composition selected from any group consisting of glycerin, propylene glycol, methyl propylene glycol, butylene glycol, pentane glycol, hexylene glycol, octyl glycol, octyl glycol and ethylhexyl glycerol.

In order to achieve the above purpose, the present invention relates to another composition containing Rutaceae plant extracts, including 0.1-2wt% Rutaceae plant extracts; 10-30wt% C1-C4 polyol system; 1-4wt% natural preservatives ; 0.5-2wt% natural thickener and 60-70wt% pure water. In one aspect, the present invention relates to another composition containing Rutaceae plant extracts, including 0.1-2wt% Rutaceae plant extracts; 2-10wt% C5-C8 polyol system; 1-4wt% natural preservatives 1-3wt% natural thickener and 60-70wt% pure water.

In one aspect, the Rutaceae plant extract is selected from the group consisting of tangerine, tangerine, sweet orange, lime, bitter orange, grapefruit, grapefruit, lemon, lime, citron, bergamot and kumquat. An extract of an ethnic group.

The C1-C4 polyol system is a composition selected from any group consisting of glycerin, propylene glycol, methyl propylene glycol and butylene glycol. The C5-C8 polyol system is a composition selected from any group consisting of pentanediol, hexanediol, octanediol and octyl glycol. The natural thickener is a composition selected from any group consisting of trixian gum, guar gum, sodium alginate and gum arabic. Depending on the purpose of use, non-natural thickeners can also be used.

The preservatives are, for example, sodium anisate and sodium acetylpropionate; caprylic hydroxamic acid and lactic acid; or glyceryl caprylate and glyceryl undecylenate. Suitable preservatives of the present invention may be acid preservatives, such as adiponic acid, dehydrated acetic acid, benzoic acid, p-hydroxybenzoic acid or salicylic acid. Alternatively, the preservative of the present invention may be a paraben preservative, such as methyl paraben, ethyl paraben, propyl paraben, butyl paraben, and paraben Isopropyl formate, isobutyl p-hydroxybenzoate or second butyl p-hydroxybenzoate. In addition, the preservative of the present invention may be methyl isothiazolinone, methyl chloroisoxazole ketone, iodopropynyl butyl carbamate, phenoxyethanol, chlorophenylglycol, triclosan, Quaternary ammonium salts or cationic surfactants.

The composition of the present invention containing Rutaceae plant extracts can be additionally added with hyaluronic acid powder, aloe extract, essential oils or essences, and other anti-aging or whitening functional materials as required. The composition containing Rutaceae plant extracts of the present invention can be antibacterial and antiviral in a short time, has lubricating and moisturizing effects, is hygienic and safe, and has a good use effect.

Figure 1 shows the pure aqueous solution of Rutaceae plant extracts (sample 1); polyol system, preservatives and natural thickeners (sample 2); and the aqueous solution of Rutaceae plant extracts, polyol systems, preservatives and natural thickeners (Sample 3), comparison of anti-HIV-1 virus tests respectively.

Figure 2 is a pure aqueous solution of Rutaceae plant extracts diluted 80 times (Sample 1); polyol system, preservatives and natural thickeners (Sample 2); and aqueous solutions of Rutaceae plant extracts, polyol systems, and preservatives Comparison with natural thickener (sample 3), respectively, to inhibit HIV-1 from infecting JLTRG CD4+ cells.

A specific example of the present invention relates to a composition containing Rutaceae plant extract, including 0.1-2wt% Rutaceae plant extract, 10-30wt% C1-C4 polyol system and 60-70wt% pure water. In another specific example, the composition containing Rutaceae plant extract includes 0.1-2wt% Rutaceae plant extract, 2-10wt% C5-C8 polyol system and 60-70wt% pure water. In a specific example, the Rutaceae plant extract is selected from the group consisting of tangerine, tangerine, sweet orange, lime, bitter orange, grapefruit, grapefruit, lemon, lime, citron, bergamot and kumquat. An extract of an ethnic group. The Rutaceae plant extract can open the cell membrane of bacteria to allow other substances to enter, which can enhance sterilization. In addition, the Rutaceae plant extract also has antiviral effects. The Rutaceae plant extract also has antibacterial and antiviral effects.

The C1-C4 polyol system is a composition selected from any group consisting of glycerin, propylene glycol, methyl propylene glycol and butylene glycol. The C5-C8 polyol system is a composition selected from any group consisting of pentanediol, hexanediol, octanediol and octyl glycol. The above-mentioned polyol such as propylene glycol has a lubricating effect. The above-mentioned glycerin can absorb moisture in the air to moisturize the skin, and can also lock the moisture in the skin to prevent it from losing, and has a moisturizing effect. In addition, these polyols have the effect of "self-preservation", which can increase the antibacterial power and strengthen the disinfection effect.

Another specific example of the present invention relates to a composition containing Rutaceae plant extract, including 0.1-2wt% Rutaceae plant extract; 10-30wt% C1-C4 polyol system; 1~4wt% preservative; 0.5 ~2wt% natural thickener and 60-70wt% pure water. In another specific example, the composition containing extracts of Rutaceae plants of the present invention includes 0.1-2wt% Rutaceae plant extract; 2-10wt% C5-C8 polyol system; 1~4wt% preservative; 1~3wt% natural thickener and 60-70wt% pure water. In a specific example, the Rutaceae plant extract is selected from the group consisting of tangerine, tangerine, sweet orange, lime, bitter orange, grapefruit, grapefruit, lemon, lime, citron, bergamot and kumquat. An extract of an ethnic group. The Rutaceae plant extract can open the cell membrane of bacteria to allow other substances to enter, which can enhance sterilization. In addition, the Rutaceae plant extract also has antiviral effects.

The C1-C4 polyol system is a composition selected from any group consisting of glycerin, propylene glycol, methyl propylene glycol and butylene glycol. The C5-C8 polyol system is a composition selected from any group consisting of pentanediol, hexanediol, octanediol and octyl glycol. The above-mentioned polyol such as propylene glycol has a lubricating effect. The above-mentioned glycerin can absorb moisture in the air to moisturize the skin, and can also lock the moisture in the skin to prevent it from losing, and has a moisturizing effect. In addition, these polyols have the effect of "self-preservation", which can increase the antibacterial power and strengthen the disinfection effect.

The natural thickener is a composition selected from any group consisting of trixian gum, guar gum, sodium alginate and gum arabic. Depending on the purpose of use, non-natural thickeners can also be used.

The preservatives are, for example, sodium anisate and sodium acetylpropionate; caprylic hydroxamic acid and lactic acid; or glyceryl caprylate and glyceryl undecylenate. Suitable preservatives of the present invention can also be acid preservatives, such as adiponic acid, dehydrated acetic acid, benzoic acid, p-hydroxybenzoic acid or salicylic acid. Alternatively, the preservative of the present invention may also be a paraben preservative, such as methyl paraben, ethyl paraben, propyl paraben, butyl paraben, and parahydroxybenzoate. Isopropyl benzoate, isobutyl p-hydroxybenzoate or second butyl p-hydroxybenzoate. In addition, the suitable preservatives of the present invention can also be methyl isothiazolinone, methyl chloroisoxazole ketone, iodopropynyl butyl carbamate, phenoxyethanol, chlorophenylglycol, Triclosan, quaternary ammonium salts or cationic surfactants.

The composition of the present invention containing Rutaceae plant extracts can be additionally added with other auxiliary materials such as hyaluronic acid powder, aloe extract or essential oils as required without affecting the antibacterial and antiviral effects. The composition containing Rutaceae plant extracts of the present invention can be antibacterial and antiviral in a short time, has lubricating and moisturizing effects, is hygienic and safe, and has a good use effect.

Example 1: Preparation of Rutaceae plant extracts into aqueous solution

In this example, three aqueous solutions of Rutaceae plant extracts with different concentrations and pH values were used for the antibacterial activity test, 0.5v% and pH 2.5, 1v% and pH 4.4 and 1v% and pH 5.2. The test data results are shown in Table 1.

According to the test data shown in Table 1, it is found that the aqueous solution of Rutaceae plant extract with 0.5v% and pH 2.5 has better antibacterial activity. If the concentration is increased to 1v%, but at the same time, in order to reduce the amount of the Rutaceae plant extract of the present invention The composition must increase the pH for vaginal stimulation or normal human skin use. As a result, it was found that the antibacterial power could not be effectively improved. Generally, the pH value of commercially available human lubricant products is 5 to 7. According to the test data, the antibacterial power at pH 5.2 is the worst of the three conditions, and it cannot reach the threshold of 99.9% antibacterial within 20 minutes. According to the normal application and use time of the human body lubricant, it is generally set to achieve 99.9% antibacterial power in 20 minutes. The Rutaceae plant extract diluted with water can have general antibacterial power at 0.5v% and pH=2.5. However, if the pH is within the range of general commercial products (pH 4-6), the antibacterial power will be significantly reduced, even at the concentration Pull up to 1v%, and the antibacterial power is only 30-40%. Therefore, the use of aqueous solutions of Rutaceae plant extracts, and the pH is within the range of use of common commercial products (pH 4-6), the antibacterial power is obviously insufficient.

Example 2: Adding a natural thickener to the aqueous solution of Rutaceae plant extracts

In this example, 1.55wt% of the Rutaceae plant extract aqueous solution was mixed with Sanxian gum (0.4wt%), guar gum (0.6wt%), hyaluronic acid powder (0.05wt%), and aloe extract (0.1wt%) , Vegetable glycerin (9.5%) and pure water are mixed to form a gel. Except for glycerin, the above are basically natural raw materials that are easy to grow bacteria. The test data results are shown in Table 2.

Table 2

According to the test data shown in Table 2, it is found that the antibacterial power is obviously decreased or there is no antibacterial power, which shows that the aqueous solution of Rutaceae plant extracts cannot be used as antibacterial and antiseptic products of gel products, let alone achieve short-term sterilization. Viral effect. Therefore, mixing the aqueous solution of Rutaceae plant extracts and natural thickeners that are easy to grow bacteria and not easy to store for a long time, such as Sanxian gum, guar gum, etc., is mixed into a gel. The results of the test data show that the antibacterial power is obviously insufficient. , And even have doubts about antibacterial and antiseptic.

Example 3: Adding an aqueous solution of Rutaceae plant extracts to a modified silicone oil based on a suitable amount of water and polyols

In this example, a 1wt% aqueous solution of Rutaceae plant extract was added to a mixture of 80wt% Dow Corning 2501 modified silicone oil, 9wt% water and 10wt% glycerin. The pH value of the formulation was 4.5. The test data results are shown in Table 3.

From the test data in Table 3, it is found that 99.9% antibacterial effect can be achieved in 20 minutes. This may be because polyols can enhance the effect of Rutaceae plant extracts, or Rutaceae plant extracts can enhance the antibacterial effect of polyols. The two have synergy Function; and Rutaceae plant extracts cannot achieve antibacterial 99.9% under water as the base, but it can be used under modified silicone oil base, which proves that Rutaceae plant extracts can be used as long as they do not grow fungi or mold or have no microorganisms. It can exert its inherent disinfection effect under the environment of its nutrient.

It can be seen from the above examples that the Rutaceae plant extracts can achieve general antibacterial effect when water is the base, but can fully exert the disinfection effect when the modified silicone oil is the base. Therefore, it is necessary to make a natural gel with disinfection effect. The antiseptic effect is better, and the ratio of antiseptic and polyol should also be adjusted to synergistic effect, in order to achieve short-term antibacterial disinfection.

Example 4: Adding an aqueous solution of Rutaceae plant extracts to water-based natural polyols, natural thickeners, and natural preservatives

In this example, the components and ratios of the aqueous solution of Rutaceae plant extracts are added to water-based polyols, natural thickeners, and preservatives, as shown in Table 4, and the antibacterial power test data results are shown in Table 5.

From the data in Table 5, it can be seen that the formula of Example 4 has an inhibitory rate of at least 99.9% for the four bacteria of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans within 20 minutes.

The following describes the anti-HIV-1 virus test of the formulation of Example 4. Figure 1 shows a pure 1.35wt% aqueous solution of Rutaceae plant extracts (sample 1); polyol system, preservatives and natural thickeners (sample 2); and aqueous solutions of Rutaceae plant extracts, polyol systems, preservatives and natural Comparison of the anti-HIV-1 virus test of the thickener (Sample 3). It can be seen from Figure 1 that sample 3 has better antibacterial, antiseptic and antiviral effects than sample 1 or sample 2.

Figure 2 is a simple 1.35wt% aqueous solution of Rutaceae plant extracts diluted 80 times (Sample 1); polyol system, preservatives and natural thickeners (Sample 2); and Rutaceae plant extracts aqueous solution and polyol system Comparison of the ability of preservatives and natural thickeners (sample 3) to inhibit HIV-1 infection of JLTRG CD4+ cells. It can be seen from Figure 2 that sample 3 has better antibacterial, antiseptic and antiviral effects than sample 1 or sample 2, and has reached 80% of the effect of azidothymidine (AZT) as a positive control drug.

Table 6 shows the 50% inhibitory concentration of sample 3 against HIV-1 infection by the dilution factor of sample 3.

The foregoing examples show that the antibacterial effect of the composition of the aqueous solution of the Rutaceae plant extract, the polyol system, the preservative and the thickening agent is higher than that of the aqueous solution of the Rutaceae plant extract alone or the addition of a natural thickener to the aqueous solution of the Rutaceae plant extract. The composition is better, and the antibacterial rate can reach 99.9%. In addition, taking the HIV-1 test as an example, the composition of the aqueous solution of Rutaceae plant extracts, polyol system, preservatives and natural thickeners (Sample 3) is more effective than the pure aqueous solution of Rutaceae plant extracts (Sample 1). ) Or the composition of polyol system, preservative and natural thickener (Sample 2) are all better, up to about 80% of the antiviral ability of the positive control drug AZT, and the experimental conditions are when the sample is diluted 80 times . Therefore, it is estimated that sample 3 has a very significant antibacterial and antiviral effect of 99.9% without dilution.

Example 5: Adding an aqueous solution of Rutaceae plant extracts to water-based polyols, natural thickeners, and preservatives

In Example 5, the components and ratios of the aqueous solution of Rutaceae plant extracts were added to water-based polyols, natural thickeners, and preservatives, as shown in Table 7. The difference between Example 5 and Example 4 is that different polyols are used, and different ratios can be used to achieve the same level of antibacterial effect.

The composition containing Rutaceae plant extracts of the present invention is suitable for various dosage forms, unlike many disinfectants that can only be made into aqueous solutions, or most cationic fungicides cannot be combined with most natural thickeners (mostly anionic) Matching, for example, the commercially available products such as water god spray, white factor and golden shield are all aqueous solutions and are not suitable for making gel or cream products.

The description of the embodiments and specific examples of the present invention should be regarded as illustrative rather than limiting the present invention as defined in the scope of the patent application. It will be easily understood that a large number of changes and combinations of the features described above can be utilized without departing from the present invention as described in the scope of the patent application. Such changes are intended to be included in the scope of the following patent applications.

Claims (6)

An antibacterial and antiviral composition composed of the following ingredients: 0.1-2wt% Rutaceae plant extract, 10-30wt% C1-C4 polyol system, 1-4wt% preservative, 0.5-2wt% natural Thickener, 60-70wt% pure water, wherein the composition has a bacteriostatic rate of at least 99.9% and an anti-HIV-1 virus efficacy of 99.9% within 20 minutes, wherein the Rutaceae plant extract is selected from orange, An extract of any group consisting of mandarin, sweet orange, lime, bitter orange, grapefruit, grapefruit, lemon, lime, citron, bergamot and kumquat, wherein the bacteria inhibited by the composition is Rosa albicans Bacteria, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. A composition for sterilizing and inhibiting viruses, composed of the following ingredients: 0.1-2wt% Rutaceae plant extract, 2-10wt% C5-C8 polyol system, 1-4wt% preservative, 1-3wt% Natural thickener, 60-70wt% pure water, wherein the composition has a bacteriostatic rate of at least 99.9% within 20 minutes and an anti-HIV-1 virus efficacy of 99.9%. Among them, the Rutaceae plant extract is selected from orange Extracts of any group consisting of citrus, orange, sweet orange, lime, bitter orange, grapefruit, grapefruit, lemon, lime, citron, bergamot and kumquat, wherein the bacteria inhibited by the composition are white Candida, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. The composition of claim 1, wherein the C1-C4 polyol system is a composition selected from any group consisting of glycerin, propylene glycol, methyl propylene glycol and butylene glycol. The composition of claim 2, wherein the C5-C8 polyol system is a composition selected from any group consisting of pentanediol, hexanediol, octanediol, and octyl glycol. The composition of claim 1 or 2, wherein the natural thickener is a composition selected from any group consisting of trixian gum, guar gum, sodium alginate and gum arabic. The composition of claim 1 or 2, wherein the preservative is sodium anisate and sodium acetylpropionate; caprylic hydroxamic acid and lactic acid; or glyceryl caprylate and glyceryl undecylenate.

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