SU621351A1 - Method of obtaining plumbagin - Google Patents

Description

(54) METHOD FOR OBTAINING PLYUMBAGIN

The invention relates to the field of pharmacology and relates to the preparation of a plumbum, which is used as an antimicrobial component in the food industry, as well as for the production of cosmetic creams.

A known method for producing plumbagine by extracting plant phyto genus G e sy tost a with an organic gyrophobic solvent, mainly petroleum. ether, treatment of the extract with dilute alkali, acidification and treatment of the ester ohm GL.

The disadvantage of this method is the limited raw material base and the low yield of the target product.

The purpose of the invention is to expand the resource base and increase the yield of the target product.

This goal is achieved by using plants of Rhodobyte E28sn sposch as a source of raw materials, before shredding roots, stalks and spice are treated with a hydrophilic salt, which has a neutral or acidic reaction, in an amount of 25-30% by weight of the latter.

A plant of the genus | REITLA EEEO1 spach (Tooth) is an annual plant. The sowing of plumbagin in August in the plant of the genus Zubnid is 1% of the fresh mass in the root, 0.36-0.60% of the fresh mass in the stems and leaves, or correspondingly: 2% by weight and 0.8-1.2% by weight of absolutely dry material. By the end of the growing season (2.5–3 months after the pueyus), the plants are removed from the soil along with the roots, and the entire biomass is processed into an I liter of HM bugs n.

Claims (1)

Due to the very high content of plumbagin in plants of the Zubnid family, to accelerate extraction from fresh biomass and to fully extract it, the plant material is crushed and mixed with a dry hydrophilic salt with neutral or slightly acidic reaction, for example, with technical sodium chloride, sodium sulfate, chloride. Kalysh. Salt is added to a 25-Chb grinded plant material in the amount of 25-3Chb plummet of the latter, mixed, shaken on a wotsuch for 30-40 minutes, then poured with ether and extracted for 4-45 minutes. The extract is drained and the plant material is extracted again for 4–45 minutes, after which the combined extracts are treated in a known manner. PlumJagagin obtained from Dental, after its recrystallization from 6O% alcohol and drying over calcium chloride, has a melting point of 75.7, S.C. Mixing with a reliable image of UOM plumbagin does not cause a melting point depression. The results of the analysis of the elementary composition,% C 69,89; 69.92 4.32: 25.75, Calculated,%: C 7O, 21; H 4.26; About 25.3 The IR spectrum of the Zyu plumbagin (5nits fully coincides in all absorption bands with the spectrum of the plumbagin) (absorption maxima at 165 O, 1617, 1594, 1460, 1368, 1337, 1262, 1235 1190, 1164, 1О58, UZ5, 839 , jeScM When measuring 128 g of fresh biomass (roots, stems and leaves) of rosar plants, ttfboi6eE oii spoiclfi and shredding in a dish, mixed with 35 g of hydrophilic salt (in this case, boiling the salt in air for 30 min, then pouring petroleum ether in the ratio of 1: 1O to the weight of the extracted biomass, taking into account the specific gravity of about 0.8i over 45 minutes, the extract is drained and the sesame the material is extracted for the second time with the same volume of petroleum ether for 45 minutes, the combined extracts are then treated in a known manner (treating the crude extract with dilute alkali, acidifying 1 alkaline solution, removing the plumbagin from it with ether.) The yield of crude plumbagin is 924 mg. After recrystallization from 55 -6O% ethanol yield of pure crystalline plumbagin 829 mg (0.64% of fresh plant mass). After twofold extraction, the plant material is subjected to the third, control extraction with petroleum ether (30% ml), and the extract content of plumbagin is determined by a known colorimetric method. It was less than 0.01%, which proves the extraction of plumbagna in the first two extractions. The proposed method provides an increase in productivity during extrusion, an increase in the yield of the Target Product, an expansion of the raw material base. Using the method will fully meet the need for PL ombagina. So, from an area of 1 hectare, with an average biomass yield of 4 tons and an average content of 0.4-O plumbagin in it, 5%, the yield of pure net product is 16-18 kg. The method of obtaining plumbagin by extracting plant raw material with an organic hydrophobic solvent, mainly petroleum ether, treating the extract with dilute alkali, acidified ether and treating it, characterized in that, in order to expand the raw material base and increase the yield of the target product, the root process will be used. before extraction, the crushed roots7 of the stems and leaves are treated with a hydrophilic salt, which has a neutral or acidic reaction, in the amount of 25-ZO% by weight: the latter. Sources of information taken into account in the examination: 1. USSR Author's Certificate No. 351552, cl. A 61 C 35/78, 1972.