MX2020009964A - Bordetella pertussis-derived protein-obtaining method including affinity chromatography process. - Google Patents

Bordetella pertussis-derived protein-obtaining method including affinity chromatography process.

Info

Publication number
MX2020009964A
MX2020009964A MX2020009964A MX2020009964A MX2020009964A MX 2020009964 A MX2020009964 A MX 2020009964A MX 2020009964 A MX2020009964 A MX 2020009964A MX 2020009964 A MX2020009964 A MX 2020009964A MX 2020009964 A MX2020009964 A MX 2020009964A
Authority
MX
Mexico
Prior art keywords
bordetella pertussis
proteins
method including
derived protein
affinity chromatography
Prior art date
Application number
MX2020009964A
Other languages
Spanish (es)
Inventor
Dong Ho Ahn
Gi Sub Choi
Jae Hoon Moon
Hyung Jin Jeon
Jong Kwan Park
Hae Ryun Kim
Bo Mi Choi
Original Assignee
Green Cross Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp filed Critical Green Cross Corp
Publication of MX2020009964A publication Critical patent/MX2020009964A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a Bordetella pertussis-derived protein-obtaining method including an affinity chromatography process. The present invention increases the production yield of PT and FHA proteins by isolating PT and FHA proteins of Bordetella pertussis through a purification process using a blue affinity column. In addition, when used, the filtration process can remarkably increase the output of the target proteins, compared to an isolation method using a size exclusion chromatography (SEC) process. Furthermore, the method using the blue affinity column for isolation of PT and FHA proteins decreased resin expense, process time, buffer consumption, and production cost and significantly increased the productivity of PT and FHA proteins.
MX2020009964A 2018-03-27 2019-03-15 Bordetella pertussis-derived protein-obtaining method including affinity chromatography process. MX2020009964A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020180035046A KR102362777B1 (en) 2018-03-27 2018-03-27 Method for obtaining bordetella pertussis related proteins comprising affinity chromatography
PCT/KR2019/003051 WO2019190092A1 (en) 2018-03-27 2019-03-15 Bordetella pertussis-derived protein-obtaining method including affinity chromatography process

Publications (1)

Publication Number Publication Date
MX2020009964A true MX2020009964A (en) 2020-10-12

Family

ID=68062325

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020009964A MX2020009964A (en) 2018-03-27 2019-03-15 Bordetella pertussis-derived protein-obtaining method including affinity chromatography process.

Country Status (7)

Country Link
JP (1) JP7031814B2 (en)
KR (1) KR102362777B1 (en)
CN (1) CN111918874B (en)
AU (1) AU2019242986B2 (en)
BR (1) BR112020019758A2 (en)
MX (1) MX2020009964A (en)
WO (1) WO2019190092A1 (en)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8512972D0 (en) * 1985-05-22 1985-06-26 Univ Glasgow Vaccine production
FR2597605B1 (en) * 1986-04-16 1989-06-23 Merieux Inst NOVEL MATERIAL FOR AFFINITY CHROMATOGRAPHY AND ITS APPLICATION TO THE SEPARATION AND PURIFICATION OF PROTEIN ANTIGENS FROM BORDETELLA GENUS
US4705686A (en) * 1986-05-09 1987-11-10 American Cyanamid Process for the preparation of acellular Bordetalla pertussis vaccine
US5101014A (en) * 1989-02-10 1992-03-31 United States Of America Process for the purification of a 69,000 da outer membrane protein of Bordetella pertussis
ES2084668T3 (en) * 1989-11-06 1996-05-16 Smithkline Beecham Biolog PROCESS.
DE19512346C1 (en) * 1995-04-01 1996-06-13 Behringwerke Ag Procedure for cleaning filament hemagglutinin and pertussis toxin
CA2621023C (en) 2005-09-01 2019-07-02 Novartis Vaccines And Diagnostics Gmbh & Co. Kg Multiple vaccination including serogroup c meningococcus
EP2415779B1 (en) 2010-08-02 2015-01-21 ratiopharm GmbH Method of producing and purifying an active soluble sialyltransferase
CN104918634A (en) 2012-10-12 2015-09-16 葛兰素史密丝克莱恩生物有限公司 Non-cross-linked acellular pertussis antigens for combination vaccines
ES2597832T3 (en) 2013-03-08 2017-01-23 Janssen Vaccines & Prevention B.V. Acellular vaccine against pertussis
CN105175517A (en) 2015-09-17 2015-12-23 北京民海生物科技有限公司 Separation and purification method for pertussis antigen

Also Published As

Publication number Publication date
JP2021517154A (en) 2021-07-15
BR112020019758A2 (en) 2021-03-02
KR102362777B1 (en) 2022-02-15
KR20190112982A (en) 2019-10-08
CN111918874A (en) 2020-11-10
WO2019190092A1 (en) 2019-10-03
CN111918874B (en) 2024-04-12
JP7031814B2 (en) 2022-03-08
AU2019242986B2 (en) 2022-03-03
AU2019242986A1 (en) 2020-10-15

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