KR20170004041A - Antioxidant or anti-aging composition comprising lettuce flower extract or its fraction - Google Patents

Abstract

The present invention relates to an antioxidant or anti-aging composition comprising a lettuce flower extract or a fraction thereof. According to the present invention, a lettuce flower extract or fraction thereof has a high content of polyphenol and flavonoids and shows high activities of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) free radical scavenging, hydroxy radical and superoxide radical scavenging, and ferric reducing/antioxidant power (FRAP). Thus, it can be used advisably for an antioxidant or anti-aging pharmaceutical composition, cosmetic composition and food.

Description

An antioxidant or anti-aging composition comprising lettuce flower extract or fraction thereof as an active ingredient,

The present invention relates to a composition for antioxidant or anti-aging, and relates to a pharmaceutical composition for antioxidant or anti-aging, a cosmetic composition, and a food containing an extract of lettuce or a fraction thereof as an active ingredient.

The basic tritiated oxygen, which is a stable molecular state generally present in the air, is a free radical which is highly reactive due to environmental and biochemical factors such as in vivo enzymes, reducing metabolism, chemicals, pollutants, It is converted to active oxygen and irreversibly destroys cellular components.

The active oxygen is an antioxidant enzyme such as superoxide dismutase (SOD), catalase, peroxidase, glutathione, vitamin C, ascorbic acid, vitamin E (tocopherol) and the like can be minimized by the action of antioxidants. However, when exposed to excessively active oxygen or defective bio-defense, the balance is broken and active oxygen causes lethal oxygen toxicity to the living body.

Excessively active oxygen in the body reacts with cellular components such as lipids, proteins, sugars, and DNA to destroy it, thereby causing aging, brain diseases such as cancer, stroke and Parkinson's disease, heart disease, ischemia, atherosclerosis, , Rheumatism, autoimmune diseases, and the like.

 As such oxidative stress is found to be an important cause of various diseases including aging, researches are actively conducted to develop antioxidants that remove active oxygen in vivo.

BHA (butylated hydroxy anisol) and BHT (butylated hydroxy toluene), which are widely used as synthetic antioxidants, have been widely used because of their effectiveness, economical efficiency and safety. However, not only general avoidance phenomena of synthetic additives but also gastrointestinal mucosa, lung, And it is known that it causes serious toxic effects on the environment and its usage is legally regulated.

Natural antioxidants, which are harmless to human body and have excellent antioxidant power, have been studied for a long time. However, natural antioxidants such as tocopherol are safe for human body, but they have the ability to inhibit oxidative chain reaction and have a disadvantage of high price have.

Therefore, it is urgently required to develop a new antioxidant which is safer, has excellent antioxidant efficacy, and is economical.

Lettuce ( Lactuca sativa ) is a first and second year herbaceous plant belonging to the Asteraceae family, and is cultivated in Europe and West Asia. The other names of lettuce are Wagar (food prime herb), Rajeong (Buhui Ward room), Chunggwangchae (Chungil Rock), Wassoon (Chonnam Chongmok), Wajae . The stem is straight, smooth and hairless, and grows up to 1 meter tall as a stick like a bamboo shoot when it is young, and branches are split a lot. The leaves are mainly edible, and the edges of the leaves are flat, wrinkled, or irregular serrated, with no sacks. Flowers bloom from June to July in yellow, flowers are positive and all are tongue-shaped flowers. There are 5 stamens, the ovary is on the bottom, and the stigma is divided into two. After the flowering, the small aquifers are oval and flat, with three protruding vertical edges and dark brown or grayish white.

Leaf, stems and seeds of lettuce are known to be effective for hypertension, urinary discomfort, hematuria, insufficient milking, relieving stress, promoting digestion, relieving hangovers, deodorizing, teeth whitening, etc. The efficacy of lettuce flowers is well known There is no bar.

On the other hand, Korean Patent No. 826,902 discloses that it is possible to selectively contain lettuce flowers as a method of manufacturing a herbal fermentation broth, and describes the efficacy of the lettuce flower itself or the efficacy of the herbal fermentation broth including lettuce flowers It is not.

Thus, the effect on the lettuce flower has not been elucidated in detail, and the antioxidative effect of the lettuce flower extract or its fraction is not known.

Accordingly, the inventors of the present invention have experimentally found that the lettuce flower extract and its fractions have an activity of removing free radicals and reducing ferric reducing / antioxidant power (FRAP) First. Therefore, a safe extract of lettuce and its fractions derived from natural materials can be used as an antioxidant composition, and thus the present invention has been completed.

Korean Registered Patent No. 826,902

It is an object of the present invention to provide a pharmaceutical composition for antioxidant or anti-aging, a cosmetic composition, and a food containing an extract of lettuce or a fraction thereof as an active ingredient.

In order to achieve the above object, the present invention provides a plant extract or a fraction thereof having a high content of polyphenols and flavonoids, a free radical elimination and a ferric reducing / antioxidant power (FRAP) The present invention provides an antioxidant or anti-aging pharmaceutical composition, a cosmetic composition, and a food containing the lettuce flower extract or a fraction thereof as an active ingredient.

The extract of Lettuce or its fraction of the present invention has a high content of polyphenols and flavonoids and is free from DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenging, ABTS (2,2'-azino-bis -6-sulfonate) radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, and ferric reducing / antioxidant power (FRAP) The extract or its fractions can be usefully used as an active ingredient in pharmaceutical compositions, cosmetic compositions, and foods for antioxidant or anti-aging purposes.

Figure 1 shows the process for the production of lettuce flower extract and fractions.

The present invention relates to a pharmaceutical composition for antioxidant or anti-aging comprising an extract of lettuce or a fraction thereof as an active ingredient.

In one aspect of the present invention, the lettuce flower extract and its fractions can be produced by a manufacturing method comprising the following steps.

a) extracting lettuce flowers with an extraction solvent;

b) filtering the extract of step a);

c) extracting the filtered extract of step b) and concentrating it under reduced pressure, followed by drying to produce an extract of lettuce; And

d) The lettuce flower extract obtained in step c) is sequentially subjected to fractionation (systematic separation) according to the polarity of the solvent by adding hexane, chloroform, ethyl acetate and butanol sequentially, and each of the separated solvent extracts is concentrated under reduced pressure to obtain a solvent And then lyophilized to prepare a fraction.

In one embodiment of the present invention, the lettuce flower of step a) may be cultivated or commercially available.

In one embodiment of the present invention, it is preferable that the extraction solvent of step (a) is water, an alcohol, or a mixture thereof.

In one embodiment of the present invention, the alcohol in the preparation process may be a C ₁ to C ₂ lower alcohol, preferably ethanol or methanol, and even more preferably 70% ethanol.

In one embodiment of the present invention, the extraction temperature is preferably 50 to 100 ° C., the extraction time is preferably 5 to 24 hours, and the extraction frequency is preferably 1 to 5 times .

In one embodiment of the present invention, the pharmaceutical composition has at least one activity of removing harmful free radicals and decreasing ferric reducing / antioxidant power (FRAP).

In one aspect of the invention, the pharmaceutical composition comprises DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenging, ABTS (2,2'-azino-bis (3- ethylbenzothiazoline- Wherein the enzyme has at least one activity selected from the group consisting of radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, and ferric reducing / antioxidant power (FRAP).

The pharmaceutical composition of the present invention may comprise a pharmaceutically acceptable carrier. The composition comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose, gelatin, . In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical composition may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations and suppositories It can have one formulation.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount.

The term "pharmaceutically effective amount " as used herein means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will vary depending on the species and severity, age, sex, , The sensitivity to the drug, the time of administration, the route of administration and the rate of excretion, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The extract or fraction may be administered at a daily dose of 1 to 100 mg / kg, preferably 1 to 10 mg / kg.

The composition of the present invention may be administered as an individual therapeutic agent or in combination with another therapeutic agent exhibiting an antioxidative or anti-aging effect, and may be administered sequentially or simultaneously with a conventional therapeutic agent. And can be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art.

In the present invention, the term "individual " means all animals, including humans, who have already developed or are capable of developing a disease that can be prevented or treated through antioxidative or anti-aging activity, and include a composition comprising the extract or fraction of the present invention By administering to an individual, the disease can be effectively prevented and treated.

The route of administration of the pharmaceutical composition may be administered through any conventional route so long as it can reach the target tissue. The composition of the present invention may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, orally, intranasally, intrapulmonarily, or rectally, though it is not intended to be limited thereto. The composition may also be administered by any device capable of transferring the active agent to the target cell.

The present invention relates to an antioxidant or anti-aging cosmetic composition containing an extract of lettuce or a fraction thereof as an active ingredient.

In one embodiment of the present invention, the lettuce flower extract of the cosmetic composition may be an extract obtained by using water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent, more preferably a mixture of water and ethanol It may be an extract that is extracted using a solvent, and more preferably, it is an extract that is extracted using 70% ethanol.

In one embodiment of the present invention, the cosmetic composition has at least one activity of removing harmful free radicals and decreasing ferric reducing / antioxidant power (FRAP).

In one aspect of the invention, the cosmetic composition comprises a radical scavenging DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical, ABTS (2,2'-azino-bis (3-ethylbenzothiazoline- , Hydroxy radical scavenging, Superoxide radical scavenging, and Ferric reducing / antioxidant power (FRAP).

The cosmetic composition may be in the form of, for example, a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) In the form of a cream, a skin, a lotion, a powder, an ointment, a spray or a cone stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition may further contain, in addition to the extract of lettuce or its fraction, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics Such as, for example, cosmetics, cosmetics, pharmaceuticals, cosmetics, pharmaceuticals,

In the cosmetic composition containing the lettuce flower extract or the fraction thereof of the present invention, the lettuce flower extract or the fraction thereof of the present invention is added to the cosmetic composition usually contained in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight .

When the lettuce flower extract or the fraction thereof of the present invention is used as an external preparation for skin, it may further contain a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, A preservative, a vitamin, a barrier, a wetting agent, a essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, a lipid vesicle or An adjuvant commonly used in the field of dermatology, such as any other ingredient conventionally used in external preparations for skin. In addition, the components can be introduced in amounts commonly used in the dermatology field.

The present invention relates to an antioxidant or anti-aging food containing an extract of lettuce or a fraction thereof as an active ingredient.

In one embodiment of the present invention, the lettuce flower extract or fraction thereof of the food may be an extract obtained by using water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent, more preferably water and ethanol The extract may be an extract obtained by using the mixture as a solvent, and more preferably an extract obtained by using 70% ethanol.

In one aspect of the present invention, the food has at least one activity of removing harmful free radicals and reducing ferric reducing / antioxidant power (FRAP).

In one aspect of the invention, the food product comprises a DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenger, an ABTS (2,2'-azino- At least one activity selected from the group consisting of Hydroxy radical scavenging, Superoxide radical scavenging, and Ferric reducing / antioxidant power (FRAP).

The food according to the present invention may include an extract of lettuce or a fraction thereof, and suitable food supplementary additives.

The term "food-aid additive " in the present invention means a component which can be added to foods in a supplementary manner, and it can be appropriately selected and used by those skilled in the art as being added to produce health functional foods of each formulation. A coloring agent and a filler, a pectic acid and a salt thereof, an alginic acid and a salt thereof, an organic acid, a protective colloid thickener, a pH adjuster, a stabilizer, a stabilizer and a stabilizer such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, A preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, etc. However, the types of the food-aid additive of the present invention are not limited by the above examples.

In one aspect of the invention, the food may be a health functional food.

The term "health functional food " in the present invention refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and rings by using raw materials and components having useful functions in the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and the health functional food of the present invention can be manufactured by the method Unlike general medicines, there is an advantage that there are no side effects that may occur when a drug is taken for a long time by using a food as a raw material, and the portability In addition, the health functional food of the present invention can be ingested as an adjuvant for enhancing antioxidant or anti-aging effect.

The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, lettuce flower extract or a fraction thereof according to the present invention is added in an amount of 1 to 10% by weight, preferably 5 to 10% by weight, of the raw material composition when the food is prepared. However, in the case of long-term ingestion intended for health and hygiene purposes or for the purpose of controlling health, the amount can also be used in the above-mentioned range.

There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary foods. The above-mentioned natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tau Martin and stevia extract, synthetic sweetening agents such as saccharine and aspartame, and the like can be used. The ratio of the natural carbohydrate is generally about 0.1 to 10 g, preferably about 0.01 to 0.1 g per 100 g of the composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples, experimental examples, and production examples. It should be understood, however, that the same is by way of illustration and example only and is not intended to limit the scope of the present invention.

[ Example ] Extraction and fractionation of sample

The lettuce flowers supplied from Hoengseong, Gangwon Province were dried at room temperature, and then 70% ethanol, which was 10 times the weight of the extract, was added. The extracts were extracted three times at 80 ° C for 8 hours. Then, the extracts were filtered using a hot condenser, Water was added to make a 70% ethanol extract of lettuce.

The 70% ethanol extract of lettuce flower was fractionated (systematically) sequentially according to the polarity of hexane, chloroform, ethyl acetate, and butanol solvent, and hexane, chloroform, ethyl acetate, butanol and water layer were separated into five fractions . Each of the separated solvent fractions was concentrated under reduced pressure to remove the solvent and dried by a freeze dryer (FIG. 1).

[ Experimental Example ]

○ Statistical processing of experimental results

The data obtained from the experiments were analyzed using a PC package (SPSS 21.0, statistical package for the social science, Chicago, IL, USA). The results were expressed as mean ± SD. The mean difference between the experimental groups was analyzed by one-way ANOVA followed by Duncan's multiple range test. Respectively.

Experimental Example  1: Determination of polyphenol and flavonoid content

To measure the total polyphenol content, 0.2 mL of the sample dissolved in distilled water was added to a test tube, 5 mL of distilled water was added, and the mixture was allowed to stand at room temperature for 3 minutes. Then, 1 mL of 10% Na ₂ CO ₃ saturated solution was added and reacted at room temperature for 1 hour. Absorbance of the supernatant was measured at 700 nm. Caffeic acid was prepared at a concentration of 0 to 100 μg / mL and analyzed by the same method as the sample. The total phenol content of the extract was calculated from the standard calibration curve.

Total flavonoid was prepared by adding 0.1 mL of 10% aluminum nitrate, 0.1 mL of 1 M potassium acetate and 4.3 mL of 80% ethanol to the 0.5 mL of the extract, mixing the mixture, incubating at room temperature for 40 minutes, nm absorbance was measured. The standard substance, quercetin, was prepared at a concentration of 0 to 100 μg / mL and analyzed in the same manner as the sample. The total flavonoid content of the extract was calculated from the standard calibration curves.

As a result, total polyphenol and flavonoid contents of lettuce flower 70% ethanol extracts were 46.86 ± 4.61 mg / g and 42.81 ± 2.06 mg / g, respectively. The total polyphenol content of the butanol fraction was 177.81 ± 10.28 mg / g, which was higher than that of the 70% ethanol extract of lettuce flower and the content of flavonoid increased to 170.27 ± 1.61 mg / g. Especially, the total polyphenol content of the ethyl acetate fraction was 298.37 ± 7.55 mg / g, which was about 6 times higher than that of 70% ethanol extract of lettuce flower, and the flavonoid content was also 175.46 ± 3.44 mg / (Table 1).

Experimental Example  2: DPPH Free radical Scatters

In order to confirm the DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenging activity, 5.6 mL of 1 × 10 ^-4 M DPPH solution was added to 0.4 mL of the sample dilution and left for 10 minutes. Were measured. The DPPH scavenging activity was expressed as the absorbance ratio of the addition group to the sample-free addition group.

As a result, the DPPH radical scavenging activity of all fractions including the 70% ethanol extract increased in a concentration dependent manner. Especially, at the concentration of 1,000 μg / mL, the 70% ethanol extract showed a scavenging activity of 40.25 ± 1.34%, the butanol fraction showed a high scavenging activity of 88.44 ± 0.08%, the ethylacetate fraction showed 92.36 ± 0.08% (Table 2). In addition, the highest activity of the extracts was observed.

Experimental Example  3: ABTS Free radical Scatters

To determine the free radical scavenging ability of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonate), ABTS (cation decolorization assay) The reaction solution was diluted with distilled water to an absorbance of 0.7 at 734 nm, and then 1 mL of the sample solution was added to 2 mL of the solution, followed by reaction at room temperature for 10 minutes. Then, 734 nm The ABTS free radical scavenging activity was expressed as the absorbance ratio of the sample with and without the sample.

As a result, the ABTS radical scavenging activity of all fractions including the 70% ethanol extract increased in a concentration dependent manner. Especially, at the concentration of 200 μg / mL, the 70% ethanol extract showed an elimination activity of 57.73 ± 0.75%, the butanol fraction had an elimination activity of 79.13 ± 0.15%, and the ethyl acetate fraction had an activity of 85.59 ± 0.30 % Of the scavenging activity (Table 3).

Experimental Example  4: Hydroxy ( Hydroxy ) Radical Scatters

To confirm the hydroxyl radical scavenging ability, 0.2 mL of 10 mM FeSO ₄ · 7H ₂ O / EDTA solution, 0.2 mL of 10 mM 2-deoxyribose, 0.2 mL of sample solution per concentration, 0.1 M sodium phosphate buffer solution 1.2 mL of sodium phosphate buffer (pH 7.4) and 0.2 mL of 10 mM H ₂ O _{2 in} that order, react at 37 ° C for 1 hour, add 1 mL of 10% TCA solution and 1 mL of 1% TBA, For 10 minutes, and then quenched, and the absorbance was measured at 532 nm. The hydroxyl radical scavenging ability of the sample was calculated according to the following equation.

As a result, hydroxy radical scavenging activity was increased in the other fractions except for the hexane fraction in a concentration dependent manner. Particularly, the 70% ethanol extract showed a scavenging activity of 29.70 ± 2.19% at the concentration of 5 mg / mL, and the butanol fraction and the water layer showed the scavenging activity at the same concentration of 41.35 ± 0.32% and 38.15 ± 2.27% ).

Experimental Example  5: Superoxide ( Superoxide ) Radical Scatters

To confirm the superoxide radical scavenging ability, each sample was added at a concentration of 0.5 mL into a 24-well plate, and 0.1 mL of 30 mM tris-HCl buffer (pH 8.0), 0.1 mM phenanthrene 0.2 mL of phenazine methosulfate (PMS), 0.2 mL of 0.5 mM nitrotetrazolium blue chloride (NBT), and 0.4 mL of 0.5 mM nicotinamide adenine dinucleotide (NADH). After incubation at room temperature for 10 minutes, absorbance was measured at 560 nm.

As a result, the 70% ethanol extract and fraction showed an increase in superoxide radical scavenging activity in a concentration dependent manner, but 70% ethanol extract, hexane fraction, chloroform fraction and water layer showed less than 50% scavenging activity. However, ethyl acetate fraction and butanol fraction showed superoxide radical scavenging activity of 53.00 ± 0.64% and 69.89 ± 0.80% at a concentration of 1,000 μg / mL, respectively (Table 5).

Experimental Example 6: Ferric reducing / antioxidant power ( FRAP ) activity (reducing power)

For ferric reducing / antioxidant power (FRAP) measurements, the ferric reducing agent (FRAP reagent) was dissolved in 25 mL of 300 mM sodium acetate buffer (pH 3.6), 40 mM HCl 2.5 mL of 10 mM 2,4,6-tris (2-pyridyl) -s-triazine (TPTZ) dissolved in 20 mM ferric chloride (FeCl ₃ ), and 3 mL of distilled water. In the experiment, 0.05 mL of each sample was added to 1.5 mL of the mixture, and the mixture was reacted at 37 ° C. for 5 minutes, and the absorbance at 593 nm was measured.

As a result, 70% ethanol extract of lettuce showed 0.405 ± 0.006 absorbance at a concentration of 1,000 μg / mL. The hexane fraction, chloroform fraction and water layer showed low absorbance of 0.3 or less at the same concentration. On the other hand, the ethyl acetate fraction and the butanol fraction exhibited an absorbance of 2.726 ± 0.012 and 1.381 ± 0.010, respectively, at the same concentration (Table 6).

[ Manufacturing example ]

Production Example 1. Preparation of antioxidant or anti-aging pharmaceutical preparation containing extract of lettuce or its fraction as an active ingredient

1-1. Sanje  Produce

Lettuce flower extract or its fraction ... 500 mg

Sucrose ......................................... 100 mg

Talc ............................................. 10 mg

The above components are powdered and mixed, and filled in an airtight container to prepare a powder.

1-2. Manufacture of tablets

Lettuce flower extract or its fractions ................... 100 mg

Lactose ............................................. 100 mg

Starch ............................................. 100 mg

Magnesium stearate .............................. 2 mg

The above components are mixed and tablets are prepared by tableting according to a conventional method for producing tablets.

1-3. Manufacture of capsules

Lettuce flower extract or its fractions ................... 100 mg

Lactose ............................................. 100 mg

Starch ............................................. 100 mg

Talc ............................................. 2 mg

Magnesium stearate ................................ Amount

The above components are mixed and filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules.

1-4. Manufacture of granules

Lettuce flower extract or its fraction ... 150 mg

Soybean extract ...................................... 50 mg

Glucose ........................................... 200 mg

Starch ............................................. 500 mg

After mixing the above components, 100 mL of 30% ethanol is added, and the mixture is dried at 60 ° C to form granules, which are filled in a capsule to prepare granules.

1-5. Pennant  Produce

Lettuce flower extract or fraction thereof 20 mg

Lactose ............................................. 1,500 mg

Glycerin ......................................... 1,500 mg

Starch ............................................. 980 mg

After mixing the above components, they are prepared so as to be 4 g per one ring according to a conventional method for producing a pellet.

1-6. Liquid  Produce

Lettuce flower extract or its fractions ................... 100 mg

Sugar ............................................. 20 g

Is isomerization ......................................... 20 g

Lemon flavor ............................................

Add purified water to the whole ............................. 100 ml

The above components are mixed according to a conventional method for preparing a liquid preparation, filled in a 100 ml brown bottle, and sterilized to prepare a liquid preparation.

1-7. Injection preparation

Lettuce flower extract or fraction thereof 0.1 mg

Sodium Metabisulfite ............................ 0.5 mg

Methylparaben ....................................... 0.8 mg

Propylparaben ..................................... 0.1 mg

Sterile sterilized water for injection ................................

The above ingredients are mixed and made into 3 ml by a conventional method, and then filled into a 3 ml capacity ampoule and sterilized to prepare an injection.

Production Example 2. Preparation of antioxidant or anti-aging cosmetic containing extract of lettuce or fraction thereof as an active ingredient

2-1. Production of flexible lotion

A soft lotion containing extract of lettuce or a fraction thereof as an active ingredient is prepared as shown in Table 7 below.

2-2. Manufacture of nutrition cream

The nutritional cream containing the lettuce flower extract or its fraction as an active ingredient is prepared as shown in Table 8 below.

Production Example 3. Preparation of antioxidant or anti-aging food containing extract of lettuce or its fraction as an active ingredient

3-1. Manufacture of flour food products

0.5 to 5.0 parts by weight of lettuce flower extract or its fractions are added to wheat flour, and the mixture is used to prepare breads, cakes, cookies, crackers and noodles.

3-2. soup  And juices

0.1 to 5.0 parts by weight of lettuce flower extract or its fractions are added to the soup and juice to prepare a health improvement meat product, noodle soup and juice.

3-3. Manufacture of dairy products

5 to 10 parts by weight of lettuce flower extract or its fractions were added to milk, and the milk was used to prepare various dairy products such as butter and ice cream.

3-4. Manufacture of Health Functional Foods

A vitamin A acetate, 70 mg of vitamin A, 1.0 mg of vitamin E, 0.13 mg of vitamin B1, 0.15 mg of vitamin B2, 0.5 mg of vitamin B6, 0.2 mg of vitamin B12, 10 mg of vitamin C, 10 mg of nicotinic acid amide, 1.7 mg of nicotinic acid amide, 50 mg of folic acid, 0.5 mg of calcium pantothenate, 1.75 mg of ferrous sulfate, 0.82 mg of zinc oxide, 25.3 mg of magnesium carbonate, 15 mg of potassium phosphate monobasic Mg, potassium citrate (90 mg), calcium carbonate (100 mg) and magnesium chloride (24.8 mg) are mixed and prepared according to a conventional method for preparing healthy foods.

3-5. Manufacture of health drinks

The extract is prepared by mixing 20 mg of the extract of Lettuce or its fraction, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of a plum concentrate, 1 g of taurine and an appropriate amount of purified water.

Claims (5)

A pharmaceutical composition for skin aging comprising an butanol or an ethyl acetate fraction of an extract obtained by extracting lettuce flowers with water, C ₁ to C ₂ lower alcohols or a mixture thereof as a solvent.
The method according to claim 1,
Wherein the lettuce flower extract is an extract obtained by using a 70% ethanol extract.
The method according to claim 1,
Wherein the pharmaceutical composition exhibits at least one activity of removing harmful free radicals and reducing ferric reducing / antioxidant power (FRAP).
A cosmetic composition for antioxidant or anti-aging use wherein the lettuce flower is a butanol or ethyl acetate fraction of an extract which is extracted with water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent as an active ingredient.
An anti-aging food comprising an butanol or ethyl acetate fraction of an extract obtained by extracting lettuce flowers with water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent.

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