JP2019182863A - Skin firmness or moisture improving composition - Google Patents

Abstract

To provide a composition having excellent functionality, using natural plant materials.SOLUTION: A lindera plant extract is used as an active ingredient of a skin firmness or moisture improving composition. The lindera plant extract preferably has an inhibitory action on collagenase and elastase. The lindera plant extract preferably contains an extract from a lindera plant with water, organic solvent, or hydrous organic solvent.SELECTED DRAWING: None

Description

  The present invention relates to a composition for improving skin firmness or moisture containing a natural plant material as an active ingredient.

  The camphor tree is a deciduous shrub that is widely distributed from the southern part of Hokkaido to Kyushu. Its branches and leaves are called coral and have long been used as a raw material for herbal medicines and folk medicines that help the digestive system. Has been. In addition, it has a good fragrance, and high-grade toothpicks to be added to Japanese sweets and essential oils collected by steam distillation are used for aroma.

  In the past, various functionalities have been reported for this black moji. For example, Patent Document 1 describes a melanin production inhibitor composed of an extract of the camphor tree barkwood bark. Patent Document 2 describes an anti-Helicobacter pylori agent containing a plant extract such as black moji as an active ingredient. Patent Document 3 describes an alcohol disorder preventive agent containing a pulverized product of one part of a camphor family such as black moji or a fat-soluble solvent extract thereof. Patent Document 4 describes a protease inhibitor containing, as an active ingredient, an extract of a plant belonging to the genus Lindera Thunb. Patent Document 5 describes a cosmetic composition characterized by containing steam distilled water of a camphor family such as black moji. Patent Document 6 describes an anti-influenza virus agent containing a plant extract such as black moji as an active ingredient. Patent Document 7 describes an antihypertensive agent characterized by containing a plant such as black moji or an extract thereof. Patent Document 8 describes a Maillard reaction inhibitor containing a plant extract such as black moji as an active ingredient. Patent Document 9 describes an IgE production-suppressing composition and an antiallergic composition characterized by containing a processed product of a plant such as black moji. Patent Document 10 describes a ceramidase activity inhibitor characterized by containing a plant extract such as black moji as an active ingredient.

JP 07-277951 A JP-A-11-001429 JP 2000-344675 A JP 2001-122728 A JP 2001-226218 A JP 2004-059463 A JP 2007-051129 A JP 2010-077123 A JP 2010-180141 A JP 2017-124984 A

  An object of the present invention is to provide a composition having excellent functionality using a natural plant material.

  As a result of intensive studies to achieve the above-mentioned object, the present inventors have found that a chromophore extract extracted from a camphoraceae chromophore has an inhibitory action on collagenase and elastase, and completed the present invention. .

  That is, the present invention firstly provides a composition for improving the firmness or moisture of the skin, characterized by containing chromomy extract as an active ingredient.

  In the above-mentioned composition for improving skin firmness or moisture, it is preferable that the chromomy extract has an inhibitory action on collagenase and elastase.

  Moreover, in the above-mentioned composition for improving skin firmness or moisture, it is preferable that the chromomy extract contains an extract of chromomy water, an organic solvent, or a water-containing organic solvent.

  In the composition for improving the firmness or moisture of the skin, it is preferable that the chromody extract contains an extract of chromody with hot water, a lower aliphatic alcohol, or a hydrous lower aliphatic alcohol.

  In the above-mentioned composition for improving skin firmness or moisture, the composition is preferably used externally.

  According to the present invention, it has been clarified that a chromophore extract extracted from a camphoraceae plant has an inhibitory action on collagenase and elastase. Thereby, it is possible to provide a composition for improving skin firmness or moisture having excellent functionality, using the chromophore extract as an active ingredient. The composition is also suitable for external use forms such as cosmetics.

5 is a chart showing the results of verifying the inhibitory action of collagenase by chromophore extract in Test Example 1. 10 is a chart showing the results of verifying the elastase inhibitory action of chromomy extract in Test Example 2.

  The present invention has been found that a chromophore extract extracted from a camphoraceae of a camphoraceae plant has an inhibitory action on collagenase and elastase, and based on this, a composition for improving skin firmness or moisture is provided. That is, for example, collagen reduction and functional decline caused by collagenase are suppressed, and elastin reduction and functional decline caused by elastase are suppressed to exert an effect of improving skin firmness or moisture. Therefore, the present invention provides a composition having functionality that acts on a living body of a human being or an animal, and hereinafter, it may be referred to as “functional composition” or simply “composition” for convenience of explanation.

  The base of the black moji extract used in the present invention may be a plant belonging to the genus Lindera, for example, black moji (Lindera umbellata Thunb.), Psyllium umbellata var. Membranacea (Maxim.) Momi. , S. cerevisiae (Lindera umbellata var. Kurodaji (Lindera benz) oin (L.) Blume), Yamadae Bee (Lindera glauca Blume), Dankobai (Lindra obtusiloba Blume), Tendai Yaku (Lindera strechnifolia (Sieb. et Zucc.) F. and others. Among these, Kuromoji (Lindera umbellata Thunb.) Is particularly preferable.

  The part of the plant is not particularly limited, and examples thereof include trunks, trunks, branches and leaves, leaves, bark, roots, rhizomes, root barks, stems, flowers, seeds, pericarp, flesh, fruits, above-ground parts, underground parts, all Examples include trees. Among these, a trunk branch is particularly preferable.

  In the present invention, an extract is prepared based on the above plant and used as an active ingredient. More specifically, chromomy extract, which is an extract of chromomy, is used as the above-mentioned functional component. As an extraction solvent used for the extraction, an appropriate solvent may be used as appropriate. Typically, water, an organic solvent, or a water-containing organic solvent is used. Examples of the organic solvent include, for example, methanol, ethanol, 1,3-butylene glycol, propylene glycol, propanol, glycerin, isoprene glycol, 1,3-propanediol, pentylene glycol and other lower aliphatic alcohols, ethyl acetate. Organic solvents compatible with water, such as acetone, chloroform, and n-hexane. These organic solvents can be used in combination of two or more. When a water-containing organic solvent is used, the content of the organic solvent can be in the range of more than 0% by volume to less than 100% by volume, but is preferably 10% by volume or more, preferably 10 to 80% by volume. Is more preferable, 20-70 volume% is still more preferable, and 30-50 volume% is especially preferable. When ethanol or hydrous ethanol is used for the extraction, hydrous ethanol having an ethanol content of 10% by volume or more is preferred, hydrous ethanol having an ethanol content of 10 to 80% by volume is more preferred, and hydrous ethanol having an ethanol content of 20 to 70% by volume. Ethanol is even more preferable, and hydrous ethanol having an ethanol content of 30 to 50% by volume is particularly preferable.

  As a specific method of extraction, a general extraction means can be employed, for example, after appropriately cutting a dried product of the trunk branch of black moji, 1 to 50 times, preferably 5 to 5 times its total mass. A 20-fold amount of extraction solvent is added, and immersion and heating extraction can be performed in the range of room temperature to the boiling point of the solvent used for about 1 to 24 hours. If necessary, extraction may be performed under pressure. After extraction, filtration may be performed as necessary, the obtained extract may be concentrated under reduced pressure, or lyophilized to remove the solvent, and the target extract is appropriately prepared. be able to. The drying means may be reduced pressure drying or spray drying. For example, when the extraction solvent is water, the extraction temperature is preferably 5 to 100 ° C, more preferably 30 to 100 ° C, and still more preferably 50 to 100 ° C. When the extraction solvent is ethanol or hydrous ethanol, the extraction temperature is preferably 5 to 70 ° C, more preferably 30 to 70 ° C, and still more preferably 50 to 70 ° C. .

  The chromophore extract obtained as described above is excellent in the effect of inhibiting the activities of collagenase and elastase, as shown in Examples described later. Therefore, it can be effectively used to improve the elasticity or moisture of the skin by acting on the living body. “Improvement” means that applying the composition according to the present invention makes the skin firmer or moistened to a better state for the applicator as compared to not applying the composition according to the present invention. It also includes preventive application to maintain a better state without breaking it.

  The composition according to the present invention may be administered parenterally, for example, for external use on the skin. The form for that is not particularly limited, such as a solution, an emulsion, a form added with a dispersant, a suspension, a stabilizer, a poultice, a lotion, an ointment, a tincture, a cream, etc. It can be used in the form.

  On the other hand, the composition according to the present invention may be administered orally, for example. The form for oral administration is not particularly limited, and the solid form, liquid form, emulsion form using the above-described chromomy extract and a base material, carrier, solvent, etc. that are acceptable for oral intake. It can be in the form of a paste, jelly, or the like. Moreover, it can be set as forms, such as a tablet, a granule, a powder, a liquid agent, a capsule. Alternatively, the above-described chromodi extract can be mixed with an appropriate carrier, preferably a fatty acid triglyceride, and filled in a soft capsule or the like in a liquid state.

  Formulation is not limited to parenteral or oral preparations, and if necessary, commonly used excipients, binders, disintegrants, lubricants, stabilizers, surfactants, dissolution agents Auxiliary agent, reducing agent, buffering agent, adsorbent, fluidizer, antistatic agent, antioxidant, sweetener, corrigent, cooling agent, sunscreen agent, flavoring agent, fragrance, fragrance, coating agent, plastic One or more preparation additives such as an agent may be appropriately selected and added.

  Specific examples of such formulation additives include crystalline cellulose, low-substituted hydroxypropylcellulose, hydroxypropylmethylcellulose, croscarmellose sodium, maltodextrin, ethylcellulose, lactose, sorbitol, silicic anhydride, and silicic acid. Magnesium, hydroxypropyl cellulose, stearic acid, oleic acid, liquid paraffin, dicalcium phosphate, dibutyl sebacate, macrogol, propylene glycol, corn starch, starch, pregelatinized starch, gelatin, popidone, crospovidone, glycerin, polysorbate 80, citrus Acid, acesulfame potassium, aspartame, sodium carbonate, talc, magnesium stearate, calcium stearate, etc. That.

  In the composition for improving skin firmness or moisture according to the present invention, the above-described chromomy extract may be used as an active ingredient, and other ingredients such as vitamins, minerals, amino acids, fatty acids, and dietary fibers may be added. .

  In the composition for improving the firmness or moisture of the skin according to the present invention, it is preferable to contain 0.001 to 99% by mass of the above-mentioned chromodi extract as an active ingredient in terms of dry matter, and 0.01 to 70%. More preferably, it is contained in an amount of 0.05 to 50% by mass.

When the composition for improving skin firmness or moisture according to the present invention is used in a form for external use by humans, the amount applied / applied is, for example, 0 per application area in terms of dry matter of the above-described chromomy extract. is preferably .01~10mg / cm ² or so, more preferably about 0.05 to 5 mg / cm ^2. In addition, when orally administered, the dose varies depending on the age and body weight, but for example, it is preferably about 1.0 mg to 50 g per day for an adult, in terms of dry matter of the above-described chromomy extract, More preferably, it is about 5.0 mg to 20 g, and still more preferably about 10 mg to 10 g.

  There is no restriction | limiting in particular as a usage form of the composition for skin firmness or moisture improvement by this invention. For example, it may be in the form of cosmetics, quasi drugs, supplements, functional foods, pharmaceuticals, and the like. These forms are not limited to human use, and may be animal use. More specifically, cosmetics, quasi-drugs, supplements, functional labeling foods, foods for specified health use, health foods, functional foods, nutritional supplements, pharmaceuticals, veterinary cosmetics, quasi-drugs for animals, animals Can be used in various product forms such as supplements for animals, health foods for animals, functional foods for animals, nutritional supplements for animals, veterinary drugs, etc., or can be used in combination with these products It is.

  The use form of the composition for improving skin firmness or moisture according to the present invention may be in the form of a cosmetic composition. That is, it can be set as the cosmetic composition for exhibiting a predetermined | prescribed functionality by mix | blending predetermined amount with the chromomy extract demonstrated above in cosmetics. Specific examples include emulsions, soaps, facial cleansers, bath additives, creams, skin lotions, suntan / sunscreen lotions, packs, shampoos, rinses, treatments, cleaning agents, and the like.

  The use form of the composition for improving skin firmness or moisture according to the present invention may be in the form of a food composition. That is, it can be made into a food composition for exhibiting a predetermined functionality by blending a predetermined amount of the above-described black moji extract into food and drink. Specifically, for example, as solid, powder, granule, various confectionery such as biscuits, cookies, cakes, snacks, rice crackers, bread, powdered beverages (powdered coffee, powdered cocoa, etc.), rice cakes , Caramel and the like, but are not limited thereto. Examples of liquid, emulsified, pasty, and jelly-like products include various products such as drinks, jelly, and mousse, and medicinal liquors, but are not limited thereto. Furthermore, the form of the composition for food addition used in order to mix | blend with these food and drink may be sufficient.

  EXAMPLES The present invention will be described more specifically with reference to the following examples. However, these examples do not limit the scope of the present invention.

[Preparation Example 1] (Hot water extract lot 1)
After cutting the dried material of the trunk branches of black moji (Lindera umbellata Thunb.), 10 times the total amount of water was added and heated, followed by immersion and heat extraction for 1 hour after boiling. After extraction, insoluble matters were removed by filtration, and the obtained filtrate was concentrated under reduced pressure, and then the solvent was removed by lyophilization to obtain a dry powdery cromozi extract powder.

[Preparation Example 2] (Hot water extract lot 2)
After cutting the dried material of the trunk branches of black moji (Lindera umbellata Thunb.), 10 times the total amount of water was added and heated, followed by immersion and heat extraction for 1 hour after boiling. After extraction, a portion of the obtained filtrate was used as an extract sample, and the rest was dried under reduced pressure to remove the solvent, thereby obtaining a dry powdered chromodi extract powder.

[Preparation Example 3] (30% ethanol extract (room temperature, 1 week))
After cutting the dried product of the trunk branch of black moji (Lindera umbellata Thunb.), 5 times its total amount of 30% ethanol / 70% water-mixture was added, and the mixture was allowed to stand at room temperature for 1 week for extraction. It filtered after extraction, the obtained filtrate was dried under reduced pressure, the solvent was removed, and the dry powder-like chromodi extract powder was obtained.

[Preparation Example 4] (30% ethanol extract (60 ° C., 3 hours))
After cutting the dried material of the trunk branches of black moji (Lindera umbellata Thunb.), 5 times its total amount of 30% ethanol / 70% water-mixture was added, and extraction was performed at 60 ° C. for 3 hours. It filtered after extraction, the obtained filtrate was dried under reduced pressure, the solvent was removed, and the dry powder-like chromodi extract powder was obtained.

[Preparation Example 5] (50% ethanol extract (60 ° C., 3 hours))
After cutting the dried product of the trunk branches of black moji (Lindera umbellata Thunb.), 5 times its total amount of 50% ethanol / 50% water-mixture was added, and extraction was performed at 60 ° C. for 3 hours. It filtered after extraction, the obtained filtrate was dried under reduced pressure, the solvent was removed, and the dry powder-like chromodi extract powder was obtained.

[Preparation Example 6] (100% ethanol extract)
After cutting the dried material of the trunk branch of black moji (Lindera umbellata Thunb.), 5 times its total amount of 100% ethanol was added, and the mixture was allowed to stand at room temperature for 1 week for extraction. After extraction, a portion of the obtained filtrate was used as an extract sample, and the rest was dried under reduced pressure to remove the solvent, thereby obtaining a dry powdered chromodi extract powder.

<Test Example 1>
Using the black moji extract powder (hot water extract) obtained in Preparation Example 1, the inhibitory action of collagenase was verified.

  For the evaluation, a collagenase assay kit (“Colageno Kit CLN-100”, Collagen Technology Workshop) was used, and the above-mentioned chromodi extract powder was dissolved in sterilized distilled water to a predetermined concentration to obtain a test sample. Then, according to the method described in the kit, the collagenase inhibitory activity of the chromophore extract powder was evaluated for collagenase (derived from tadpoles).

  Specifically, according to the method described in the kit, after preparing a sample solution in which the enzyme solution diluted to 1 unit / mL and the test sample are mixed at 9: 1, 100 μL of the sample solution and the substrate solution (A Liquid: Liquid B = 1: 1) 100 μL was mixed and reacted at 35 ° C. for 120 minutes. Thereafter, 5 μL of the reaction stop solution (C solution) was added and stirred, and then the supernatant obtained by centrifugation at 3000 rpm for 10 minutes was dispensed 200 μL at a time into a black 96-well microplate, and the excitation wavelength was measured using a microplate reader. The fluorescence intensity at 495 nm / fluorescence wavelength 520 nm was measured.

  The collagenase inhibition rate was calculated by the following formula (1).

Collagenase inhibition rate (%) = (1−A / B) × 100 (1)
(In Formula (1), A is the fluorescence value of the test sample addition system, and B is the fluorescence value of the test sample non-addition system (control: total color development).)
IC ₅₀ (50% inhibitory concentration) was calculated based on the relationship between the concentration of the test sample added to the collagenase reaction system and the inhibition rate at that time according to a conventional method.

  The results are shown in Table 1 and FIG.

As a result, as shown in Table 1 and FIG. 1, the black moji extract powder (hot water extract) inhibited the collagenase activity in a concentration-dependent manner. The IC ₅₀ value was 0.029 mg / mL.

<Test Example 2>
Using the black moji extract powder (hot water extract) obtained in Preparation Example 1, the inhibitory action of elastase was verified.

  For the evaluation, an elastase assay kit (“Sensolyte Green Elastase Assay Kit”, AnaSpec) was used, and the above-mentioned chromodi extract powder was dissolved in sterilized distilled water to a predetermined concentration to prepare a test sample. Then, according to the method described in the kit, elastase inhibitory activity by the chromodi extract powder was evaluated for elastase (derived from porcine spleen).

  Specifically, according to the method described in the kit, 10 μL of a test sample and 40 μL of an enzyme solution were placed in a black 96-well microplate, stirred, and allowed to stand at 37 ° C. for 10 minutes. Thereafter, 50 μL of a substrate solution previously incubated at 37 ° C. was added and reacted at 37 ° C. for 60 minutes. After 60 minutes, the reaction solution was measured for fluorescence intensity at an excitation wavelength of 494 nm / fluorescence wavelength of 538 nm.

  The elastase inhibition rate was calculated by the following formula (2).

Elastase inhibition rate (%) = (1−A / B) × 100 (2)
(In Formula (2), A is the fluorescence value of the test sample addition system, and B is the fluorescence value of the test sample non-addition system (control: total color development).)

  The results are shown in Table 2 and FIG.

  As a result, as shown in Table 2 and FIG. 2, the black moji extract powder (hot water extract) inhibited the elastase activity in a concentration-dependent manner.

<Test Example 3>
The inhibitory action of collagenase was verified using the chromophore extract or extract powder obtained in Preparation Examples 2-6. The test was conducted in the same manner as in Test Example 1 except that collagenase (derived from tadpole) was replaced with collagenase (recombinant derived from E. coli).

  The results are shown in Table 3. Table 3 also shows the yields when the chromomy extract or extract powder was prepared in Preparation Examples 2-6.

As a result, as shown in Table 3, even with extraction with ethanol or water-containing ethanol, chromomy extract inhibited collagenase activity in a concentration-dependent manner. In addition, as can be seen from the IC ₅₀ value, when compared with the same reaction concentration, the chromomy extract was more markedly inhibited by collagen or hydrous ethanol than the hot water extraction.

<Test Example 4>
In the same manner as in Preparation Examples 2 to 6, the inhibitory action of elastase was verified using a chromophore extract or extract powder obtained in another lot. The test was conducted in the same manner as in Test Example 2 except that IC ₅₀ (50% inhibitory concentration) was calculated as in Test Example 1.

  The results are shown in Table 4.

As a result, as shown in Table 4, even with extraction with ethanol or hydrous ethanol, chromodi extract inhibited the elastase activity in a concentration-dependent manner. Further, as can be seen from the IC ₅₀ value, when compared with the same reaction concentration, the chromomy extract was more markedly inhibited by elastase or hydrous ethanol than the hot water extraction.

(Prescription Example 1)
An emollient cream containing a black moji extract with the following composition was prepared.
(1) Emollient cream:
(Composition) (Parts by weight)
Beeswax 2.0
Stearyl alcohol 5.0
Stearic acid 8.0
Squalane 10.0
Self-emulsifying propylene glycol 3.0
Monostearate polyoxyethylene cetyl ether (20EO) 1.0
Fragrance 0.5
Antioxidant Trace amount of preservative Trace amount of propylene glycol 4.8
Glycerin 3.0
Sodium hyaluronate 0.1
Chromoji extract powder of Preparation Example 0.1
Triethanolamine 1.0
Purified water 61.5
Total amount 100.0

(Prescription example 2)
An emulsion foundation containing a black moji extract with the composition shown below was prepared.
(2) Emulsion foundation:
(Composition) (Parts by weight)
Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propyl paraoxybenzoate Trace purified water 64.1
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 3.8
Sodium hyaluronate 0.1
Chromoji extract powder of Preparation Example 0.1
Triethanolamine 1.1
Methyl parahydroxybenzoate Trace amount of titanium oxide 8.0
Talc 4.0
Coloring content Trace fragrance Trace squalane 10.0
Total amount 100.0

(Prescription Example 3)
A cocoon extract containing chromomy extract was prepared with the composition shown below.
(3) 飴:
(Composition) (Parts by weight)
Powdered sorbitol 89.70
Fragrance 0.25
Chromoji extract powder of Preparation Example 10.00
Sorbitol seed 0.05
Total amount 100.00

(Prescription Example 4)
A gum containing a chromodis extract having the composition shown below was prepared.
(4) Gum:
(Composition) (Parts by weight)
Gum base 20.00
Calcium carbonate 2.00
Stevioside 0.10
Chromoji extract powder of Preparation Example 10.00
Lactose 66.90
Fragrance 1.00
Total amount 100.00

(Prescription Example 5)
A caramel containing a black moji extract with the composition shown below was prepared.
(5) Caramel:
(Composition) (Parts by weight)
Granulated sugar 32.00
Minamata 20.00
Milk powder 30.00
Hardened oil 4.00
Salt 0.60
Perfume 0.03
Water 3.37
Chromoji extract powder of Preparation Example 10.00
Total amount 100.00

(Prescription Example 6)
A carbonated beverage containing chromomy extract with the composition shown below was prepared.
(6) Carbonated drink:
(Composition) (Parts by weight)
Granulated sugar 8.00
Concentrated lemon juice 1.00
L-ascorbic acid 0.10
Citric acid 0.09
Sodium citrate 0.05
Coloring agent 0.05
Carbonated water 80.71
Chromoji extract powder of Preparation Example 10.00
Total amount 100.00

(Prescription Example 7)
Juice containing chromomy extract with the composition shown below was prepared.
(7) Juice:
(Composition) (Parts by weight)
Frozen concentrated orange juice 5.00
Fructose glucose liquid sugar 1.00
Citric acid 0.10
L-ascorbic acid 0.09
Chromoji extract powder of Preparation Example 10.00
Fragrance 0.20
Dye 0.10
Water 83.51
Total amount 100.00

(Prescription Example 8)
A lactic acid bacteria beverage containing a black moji extract with the composition shown below was prepared.
(8) Lactic acid bacteria beverage:
(Composition) (Parts by weight)
Milk solid 21% fermented milk 14.76
Fructose dextrose liquid sugar 13.31
Pectin 0.50
Citric acid 0.08
Fragrance 0.15
Water 61.20
Chromoji extract powder of Preparation Example 10.00
Total amount 100.00

(Prescription Example 9)
An alcoholic beverage containing a black moji extract with the composition shown below was prepared.
(9) Alcoholic beverages:
(Composition) (Parts by weight)
50% ethanol 32.00
Sugar 8.60
Fruit juice 2.40
Chromoji extract powder of Preparation Example 10.00
Water 47.00
Total amount 100.00

Claims (5)

  A composition for improving skin firmness or moisture, characterized by containing chromomy extract as an active ingredient.   The composition for improving skin firmness or moisture according to claim 1, wherein the black moji extract has an inhibitory action on collagenase and elastase.   The composition for improving skin firmness or moisture according to claim 1 or 2, wherein the chromody extract contains an extract of chromody water, an organic solvent, or a water-containing organic solvent.   The composition for improving skin firmness or moisture according to claim 1 or 2, wherein the chromomy extract contains an extract of chromid hot water, a lower aliphatic alcohol, or a hydrous lower aliphatic alcohol.   The composition for improving skin firmness or moisture according to any one of claims 1 to 4, which is for external use.