KR101885195B1 - Cosmetic Composition with Fermentative Extract of Osmanthus fragrans - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermented extract of Mokashi. The extract according to the present invention can be effectively used for antioxidant, anti-inflammation, promotion of collagen synthesis, improvement of skin wrinkles, moisturizing, whitening, and skin barrier improvement.

Description

[0002] Cosmetic Composition with Fermentative Extract of Osmanthus fragrans [0003]

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition comprising a fermented extract of Mokashi as an active ingredient.

Skin aging can be caused by active oxygen species activated by ultraviolet rays, stress, disease states, environmental factors, wounds, and age. When such conditions are intensified, the antioxidant defense network in the living body is destroyed, and cells and tissues are damaged, thereby promoting skin aging. Specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. The oxidation of protein breaks down collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are skin connective tissues, causing severe inflammation reaction and affect skin elasticity. When it gets worse, mutation of DNA leads to mutation, cancer, and immune function.

Therefore, it is necessary to protect the cell membrane by eradicating reactive oxygen species that are mediated by the body's metabolic process, ultraviolet radiation, inflammatory reaction, and regenerate the already damaged cells by active metabolism to proliferate the cells. It can restore and maintain healthy skin.

Meanwhile, in recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

The inventors of the present invention have studied the applicability of various natural products to cosmetics. As a result, they have found the effect of improving the antioxidant, anti-inflammation, collagen synthesis, skin wrinkle, moisturizing, whitening, Completed.

Korean Patent Laid-Open No. 10-2015-0010778 discloses a skin care composition containing extracts of plants belonging to the genus Osmanthus to reduce fibroblast collapse. Korean Patent Laid-Open No. 10-2014-0024877 describes the antioxidative effect of plant extracts such as Osmanthus fragrans, tyrosinase inhibitory effect, collagen synthesis effect, and the like.

The present inventors have provided a cosmetic composition having an antioxidant, anti-inflammation, promotion of collagen synthesis, skin wrinkle improvement, moisturizing and skin barrier improvement, which is applicable to skin cosmetics and is harmless to human body and has excellent safety, .

The present invention is based on the viewpoint of Osmanthus fragrans fermented extract.

Preferably, the cosmetic composition may be for antioxidation.

Preferably, the cosmetic composition may be anti-inflammatory.

Preferably, the cosmetic composition may be for improving skin wrinkles.

Preferably, the cosmetic composition may be used for whitening.

Preferably, the cosmetic composition may be for skin moisturizing.

Preferably, the cosmetic composition may be for skin barrier improvement.

Preferably, the Mokashi fermentation extract may be obtained by fermenting Mokusama extract with lactic acid bacteria using Bifidobacterium .

Preferably, the Moss fermented extract may be added in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Or a pharmaceutically acceptable salt thereof.

INDUSTRIAL APPLICABILITY The present invention can provide a natural extract which is applicable to skin cosmetics and which is harmless to the human body and has excellent safety, and a cosmetic composition containing it as an active ingredient.

Further, the present invention can provide a cosmetic composition having an extract of Mokashi fermented extract and an effective ingredient thereof and having antioxidant, anti-inflammation, promotion of collagen synthesis, improvement of skin wrinkles, moisturizing, whitening, and skin barrier improvement.

The present inventors have conducted studies to prepare extracts from various natural materials as a raw material by various extraction methods and to select substances having excellent effects such as antioxidation, anti-inflammation, promotion of collagen synthesis, improvement of skin wrinkles, moisturizing and skin barrier improvement . As a result, it has been found that the Mokashi fermented extract is most suitable for the purpose of the present invention described above.

Osmanthus fragrans belongs to the evergreen tree of the dicotyledonous bracken aspen tree, native to China, and to the south of Korea. It is well known as a flower blooming in winter around October, and is characterized by a fragrant fragrance.

The cosmetic composition of the present invention contains a fermented extract of Mokashiku and exhibits excellent antioxidant, anti-inflammation, promotion of collagen synthesis, improvement of skin wrinkles, moisturizing, whitening, skin barrier improvement.

In the present invention, the extract can be produced according to a conventional method known in the art. Specifically, the paste can be dried and pulverized, and then, using conventional solvents, under the conditions of ordinary temperature and pressure. Specifically, the extract can be extracted by using a solvent selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, , Fruit, leaf or stalk using the above-mentioned solvent, and then mixing them.

The solvent is preferably an alcohol, more preferably ethanol. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, most preferably about 7 times the weight of the dried material.

On the other hand, the extract according to the present invention includes not only the extract obtained by the above-mentioned solvent extraction method, but also the extract obtained through a usual purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, It is interpreted that the fraction is also included in the extract of P. pastoris of the present invention.

In addition, the extract of P. pastoris according to the present invention may be prepared into a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.

The extract can be prepared by fermenting the extracts of the present invention with at least one kind selected from the group consisting of lactic acid bacteria. Specifically, the extract can be prepared by the following method. First, 1 to 5% by weight of glucose may be added as a carbon source to the extract, and then diphosphate may be further added to sterilize the extract. And cultured inoculated with a culture solution of Bifidobacterium so as to have a concentration of 10 g / L. The culture can be carried out using a 5 L fermenter for 1 to 7 days at 20 to 40 ° C while maintaining the pH at 5-7. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented product is firstly removed from the culture, and the fermented product is refluxed with ethanol to make a final 70% (v / v) ethanol aqueous solution, cooled, and filtered. When the filtration is completed, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure at 50 ° C or lower or freeze-dried to produce the present invention.

According to a preferred embodiment of the present invention, the fermented extract is added to the cosmetic composition in an amount of 0.0001 to 30.0% by weight, preferably 0.001 to 25.0% by weight, and most preferably 0.1 to 20.0% by weight, ≪ / RTI > When the content of the extract is less than 0.0001% by weight, the effect of antioxidant, anti-inflammation, promotion of collagen synthesis, improvement of skin wrinkles, moisturizing, whitening and skin barrier is insignificant.

Meanwhile, the cosmetic composition of the present invention may contain, as an active ingredient, the components commonly used in cosmetic compositions in addition to the above-mentioned fermented extract of Mokashi, and may contain conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, , And a carrier.

Meanwhile, the cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. However, the present invention is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate , Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the cosmetic composition of the present invention is in the form of a suspension, the carrier component may include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

On the other hand, the extract of the present invention shows an antioxidative effect, anti-inflammatory effect, collagen synthesis effect and skin whitening effect. In addition, the cosmetic composition prepared by containing the moxa fermented extract having such an effect as an active ingredient showed excellent skin moisturizing effect and skin barrier improvement effect.

Hereinafter, the present invention will be described in more detail with reference to the following Production Examples. However, the following Production Examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to the following Production Examples.

[ Manufacturing example  Preparation of 1: Pastoral  Preparation of fermented extract]

The flower part of the necklace was washed with purified water, dried, and then pulverized and made fine using a sieve of 100 mesh. 70% ethanol was added to make 100g / L of the crushed product, and the mixture was refluxed three times for 5 hours. After cooling, it was filtered with whatman # 3 filter paper and concentrated under reduced pressure and freeze- The extract was prepared.

The prepared extract was added to the lactic acid bacteria culture to a concentration of 10 g / L. To this was added glucose as a carbon source and diphotassium phosphate as a pH controller. Bifidobacterium was used as a lactic acid bacterium and added at 50,000 cfu / L per culture. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 5-7. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

[ Comparative Manufacturing Example  Preparation of 1: Pastoral  Preparation of extract]

Mackerel extract was prepared as a comparative example of Mackerel fermented extract. The flower part of the necklace was washed with purified water, dried, and then pulverized and made fine using a sieve of 100 mesh. 70% ethanol was added to make 100g / L of the crushed product, and the mixture was refluxed three times for 5 hours. After cooling, it was filtered with whatman # 3 filter paper and concentrated under reduced pressure and freeze- The extract was prepared.

[ Experimental Example  One: Pastoral Extracts and Parsnips  Fermented extract DPPH method  Evaluation of antioxidant use]

The free radical scavenging activity of each of the extracts obtained in Preparation Example 1 and Comparative Preparation Example 1 (31.5, 62.5, 125, 250, 500 and 1000 ㎍ / ml) .

The DPPH assay measures absorbance at 540 nm to the extent that the inhibitor decolorizes the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical). The used samples are shown in Table 1 below, and the free radical scavenging activity was measured using the following equation (1). The control group was Epigallocatechin gallate (EGCG).

material Control group Blank of C Exp. Group Blank of E A DPPH (MeOH) 180 (180) 180 (180) B Sample (solvent) (20) (20) 20 20

A: Abs of experimental group

B: Abs blank of experimental group

C: Abs of control group

D: Abs of blank of control group

Free radical scavenging activity Production Example 1 Comparative Preparation Example 1 EGCG 25uM - - 54.34 0.001 wt% 40.55 20.93 - 0.002 wt% 69.25 34.09 - 0.005 wt% 85.49 43.12 - 0.01 wt% 94.78 58.09 -

As shown in Table 2, the extract of Moksha fermentation showed better free radical scavenging activity than the extract of Mokpo extract. Therefore, it was confirmed that the extract of the present invention has excellent antioxidative effect compared to the extract of P. pastoris.

[ Experimental Example  2: Pastoral Extracts and Parsnips  Evaluation of cell viability of fermented extract]

Toxicity of the extracts obtained from Preparation 1 and Comparative Example 1 on the cell viability was determined. Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) reagent.

HDF was dispensed into 96-well plates at a concentration of 1 x 10 ⁴ cells / well and cultured at 37 ° C and 5% CO ₂ for 24 hours. The culture medium was removed and the sample was cultured for 24 hours in a concentration-treated medium. The medium was removed and washed twice with PBS (phosphate buffered saline). MTT was dissolved in PBS at 5 mg / mL, and 50 μL was added and cultured at 37 ° C and 5% CO ₂ for 2 hours. DMSO was added to each well (100 μL), stirred for 10 minutes, and then absorbance was measured at 540 nm.

The measurement results are shown in Table 3 below. Table 3 shows no cytotoxicity at all concentrations of each sample. From this, it can be confirmed that the extract of P. pastoris according to the present invention is harmless to the human body and has excellent safety.

Cell survival rate (%) Name of sample Treatment concentration (PPM) Cell survival rate (%) Production Example 1 50 101.8 100 102.3 Comparative Preparation Example 1 50 99.8 100 100.2

[ Experimental Example  3: Pastoral Extracts and Parsnips  Evaluation of anti-inflammation of fermented extract (5- Lipoxygenase  Inhibition)]

5-Lipoxygenase inhibitory activity of the extract of Mokpo fermentation extract of Preparation Example 1 and the extract of Mokpo extract of Comparative Preparation Example 1 was evaluated to evaluate the anti-inflammatory effect.

Lipoxygenase produces a variety of substances from arachidonic acid that act as various chemical mediators involved in inflammatory and allergic reactions in vivo. Therefore, a substance that inhibits lipoxygenase may result in inhibiting the production of various chemical mediators, so it can be assumed that it is effective for allergy. The activity level of lipoxygenase is an experiment to measure the degree of peroxide formation using substrate and enzyme.

The used samples were tested as shown in Table 4 below and the 5-lipoxygenase inhibitory activity was measured using the formula (2). The control group was nordihydroguaiaretic acid (NDGA).

A: Abs of experimental group

B: Abs blank of experimental group

C: Abs of control group

D: Abs of blank of control group

material Control group Blank of C Exp. Group Blank of E A Buffer solution 2ml 2ml 2ml 2ml B Sample (solvent) (20 [mu] l) (20 [mu] l) 20ul 20ul C Enzyme 40ul - 40ul - D Substrate 70ul 70ul 70ul 70ul

Production Example 1 Comparative Preparation Example 1 NDGA 250 nM - - 51.55 0.001 wt% 32.18 13.24 - 0.002 wt% 38.14 15.74 - 0.005 wt% 59.33 19.46 - 0.01 wt% 64.43 25.31 -

As shown in the results of Table 5, the extract of Mokpo fermented extract was superior to the extract of Mokpo extract in terms of inhibition of 5-lipoxygenase. In general, the higher the inhibitory effect of 5-lipoxygenase is, the more anti-inflammatory effect is obtained.

[ Experimental Example  4: Pastoral Extracts and Parsnips  Evaluation of collagen synthesis amount of fermented extract]

The increase rate of collagen biosynthesis of the extracts of Mokushi fermentation extract and Mokusama extract obtained in Preparation Example 1 and Comparative Preparation Example 1 was evaluated.

HDF was added to a 24-well plate at a concentration of 5 x 10 ⁴ cells / well and cultured at 37 ° C and 5% CO ₂ for 24 hours. Serum-free DMEM medium supplemented with the extract prepared according to Preparation Example 1 and Comparative Preparation Example 1 and a control group were further cultured for 24 hours in a serum-free DMEM medium containing no Mokshu fermentation extract or Mokpo extract. After the incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / ml, Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following formula (3), and the control group was L-ascorbic acid (L-AA).

Production Example 1 Comparative Preparation Example 1 L-AA 0.3mM - - 131.51 0.001 wt% 126.65 111.22 - 0.002 wt% 142.38 122.54 - 0.005 wt% 164.44 133.65 - 0.01 wt% 185.35 153.74 -

As shown in the results of Table 6 above, the extract of the present invention showed better collagen synthesis than the extract of P. pastoris.

[ Experimental Example  5: B16F1  Measurement of inhibitory effect on melanin synthesis using melanocytes]

In Experimental Example 5, arbutin known as a whitening agent was used as a comparative sample and B16F1 melanocyte was used for confirming the melanin synthesis inhibitory effect of the Moxa fermented extract and the Moxa extract obtained in Preparation Example 1 and Comparative Preparation Example 1.

The B16F1 melanocyte used in Experimental Example 5 is a cell line derived from a mouse, and is a cell that secretes a melanin pigment called melanin. The inhibitory effect of B16F1 melanin on melanin synthesis was measured as follows.

B16F1 melanocytes were plated at a concentration of 2 x 10 ⁶ per well in a 6-well plate, and after attaching the cells, the samples were treated at a concentration not causing toxicity and cultured for 72 hours. After culturing for 72 hours, cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan (R Lotan and D Lotan, Cancer Res, 40: 3345-3350, 1980). Cell pellets were washed once with PBS and then 1 ml of homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin, and the absorbance of melanin was measured at 405 nm with a microplate reader. Melanin was quantified The percent inhibition of melanin formation in the sample was measured. The inhibition rate (%) of melanin formation of B16F1 melanocytes was calculated by the following equation (4).

A: Amount of melanin in wells to which no sample was added

B: Amount of melanin in the well to which the sample was added

Comparative Preparation Example 1 Production Example 1 Arbutin 0.2 wt% - - 53.2 0.001 wt% 13.24 46.64 - 0.002 wt% 21.54 69.28 - 0.005 wt% 33.63 78.44 - 0.01 wt% 42.74 86.35 -

The inhibitory effect of melanin on B16F1 melanocytes was measured and the results are shown in Table 7. The extract of P. pastoris prepared in Preparation Example 1 showed better efficacy than arbutin. It was confirmed that it had better whitening effect than Comparative Preparation Example 1.

From the above results, it was possible to confirm the excellent inhibitory effect on the melanin synthesis of the fermented extract of P. pastoris, and it was found that the excellent whitening effect of the composition for external application for skin of the present invention was confirmed.

[ Prescription example  And Comparative prescription example  Produce: Pastoral  Fermented extract Cosmetic  Produce]

The cosmetic composition containing 20.0% by weight of the extract of Moksha Kagaku of Preparation Example 1 was prepared according to the composition shown in Table 8 below and referred to as Prescription Example 1, and the cosmetic composition containing the extract of Mokashukai was referred to as Comparative Prescription Example 1.

ingredient Prescription Example 1
(weight%) Comparative Prescription Example 1
(weight%) Production Example 1 20.0 - Comparative Preparation Example 1 - 20.0 1,3-BG 10.0 10.0 glycerin 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl acetate 3.0 3.0 Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0 Squalane 3.1 3.1 Macadamia nut oil 2.5 2.5 Polyahyuvait 60 1.6 1.6 Azithromycin 1.6 1.6 Propylparaben 0.6 0.6 Carboxyl vinyl polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Balance Balance system 100 100

[ Experimental Example  6: Pastoral  Skin of fermented extract Moisture power  Improvement effect]

The following experiment was carried out to examine the effect of the cosmetic composition containing the fermented extract according to the present invention on the skin moisturizing effect. Twenty-five to twenty-four patients without skin disease were divided into two groups of 20 patients per group. The nutritional cream of the cream of Prescription Example 1 and Comparative Prescription Example 1 prepared in Table 8 was divided into two groups Month and then applied to the face and forehead. The skin conductivity was measured by using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 9 below.

sample After 1 week after 2 weeks After 4 weeks Prescription Example 1 52 55 59 Comparative Prescription Example 1 26 35 38

As shown in the results of Table 9, in the case of Prescription Example 1, which is a cosmetic containing the fermented extract of P. thunbergii according to the present invention, the rate of skin conductivity increase was much superior to that of Comparative Prescription Example 1. Since the skin conductivity is generally proportional to the skin moisture content, the above results confirm that the cosmetic composition containing the fermented extract of Moss cultivar keeps the skin moisture content higher than that of the cosmetic composition not containing it.

[ Experimental Example  7: Pastoral  Effect of fermented extract on skin barrier improvement]

In order to examine the skin barrier improvement effect of the cosmetic composition containing the fermented extract of Moss, the present invention was carried out as follows. In the present experimental example, the cosmetic materials of Table 8 were evaluated by conducting a comparative experiment using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) for transepidermal water loss (TEWL) in humans. Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1 hour, 2 hours, 4 hours, and 6 hours after applying 1cm downward to the right side of 3cm from the eyes The results are shown in Table 1. The transdermal water loss was measured using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) three times and the average value was evaluated.

division 1 hours 2 hours 4 hours 6 hours Prescription Example 1 9.2 7.8 6.4 5.2 Comparative Prescription Example 1 22.1 20.0 16.2 15.9

As shown in Table 10, as a result of measuring the amount of transdermal water loss, the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied cream containing Mokpo fermented extract, there was.

Claims (10)

A cosmetic composition comprising an extract of Bombyx mori ( Bombyx mori ) obtained by extracting flower part of Osmanthus fragrans with ethanol and then fermenting with Bifidobacterium,
Wherein the cosmetic composition has at least one effect selected from the group consisting of an antioxidative effect, an anti-inflammatory effect, a skin wrinkle improving effect, a skin whitening effect, a skin moisturizing effect and a skin barrier improving effect. The method according to claim 1,
The cosmetic composition according to claim 1, wherein the fermented extract is from 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the cosmetic composition has one formulation. delete delete delete delete delete delete delete