KR101239320B1 - Composition comprising extract of Momordica charantia for anti-cancer - Google Patents
Composition comprising extract of Momordica charantia for anti-cancer Download PDFInfo
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- KR101239320B1 KR101239320B1 KR1020100104085A KR20100104085A KR101239320B1 KR 101239320 B1 KR101239320 B1 KR 101239320B1 KR 1020100104085 A KR1020100104085 A KR 1020100104085A KR 20100104085 A KR20100104085 A KR 20100104085A KR 101239320 B1 KR101239320 B1 KR 101239320B1
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Abstract
본 발명은 여주 추출물을 유효성분으로 포함하는 항암 조성물에 관한 것으로서, 보다 구체적으로 여주 추출물을 유효성분으로 포함하는 항암 조성물 및 암 질환 예방 또는 개선용 기능성 건강식품에 관한 것이다. 본 발명에 따른 여주 추출물은 다양한 암세포의 증식을 억제하는 활성이 우수할 뿐만 아니라 인체 유해균에 대한 항균 활성도 우수한 특징이 있으므로 암 질환의 예방 및 치료를 위한 치료제와 더불어 건강기능식품으로 유용하게 사용할 수 있는 효과가 있다.The present invention relates to an anticancer composition comprising an extract of Yeoju as an active ingredient, and more particularly, to an anticancer composition comprising an extract of Yeoju as an active ingredient and a functional health food for preventing or improving cancer diseases. Yeoju extract according to the present invention is not only excellent in inhibiting the proliferation of various cancer cells, but also has excellent antimicrobial activity against human harmful bacteria, and thus can be usefully used as a health functional food with a therapeutic agent for the prevention and treatment of cancer diseases. It works.
Description
본 발명은 여주 추출물을 유효성분으로 포함하는 항암 조성물에 관한 것이다. 보다 구체적으로, 본 발명은 암세포에 대해 증식억제 효과를 가지는 여주 추출물을 유효성분으로 포함하는 항암 조성물 및 건강기능식품에 관한 것이다.
The present invention relates to an anticancer composition comprising the extract of Yeoju as an active ingredient. More specifically, the present invention relates to an anticancer composition and health functional food comprising an extract of Yeoju having an antiproliferative effect on cancer cells as an active ingredient.
암은 전 세계적으로 현대인의 사망원인 1위 질병으로서, 한국인 암 사망률은 세계 상위 수준인 것으로 알려져 있다. OECD가 2년에 한번씩 30개 회원국의 보건의료 상황을 조사해 발표한 내용을 따르면 한국인의 전체 암에 의한 사망률은 인구 10만명당 242명으로 OECD 회원국 중 4위로 높았다. 이는 인구의 평균수명은 늘어난 것에 비해 건강한 삶을 보장하기에는 아직 턱없이 미흡하다는 결과를 반영한다. 암은 증식과 억제가 조절되지 않는 비정상적인 세포들이 통제되지 못하고 과다하게 증식할 뿐만 아니라 주위 조직 및 장기에 침입하여 종괴 형성 및 정상 조직의 파괴를 초래되는 질병으로, 억제가 안되는 세포의 증식으로 정상적인 세포와 장기의 구조와 기능을 파괴하기 때문에 그 진단과 치료의 중요성이 더 강조되고 있다. 암은 어느 조직에서나 발생할 수 있지만, 가능성은 제한되어 있다. 예를 들어 유방암의 경우에는 좌측 상단에는 40%의 발병률이 있으나 우측 하단에는 20% 정도에 지난다.Cancer is the number one cause of death among modern people worldwide, and Korean cancer mortality rates are known to be among the highest in the world. Once every two years, the OECD surveyed and published the health care situation in 30 member countries, and the total mortality rate of cancer among Koreans was 242 per 100,000 population, the fourth highest among OECD countries. This reflects the fact that the average life expectancy of the population is increased, but it is still insufficient to guarantee a healthy life. Cancer is a disease in which abnormal cells, whose growth and suppression are not regulated, proliferate uncontrolled and excessively proliferate, invade surrounding tissues and organs, resulting in mass formation and destruction of normal tissues. The importance of diagnosis and treatment is emphasized because it destroys the structure and function of organs and organs. Cancer can occur in any tissue, but the possibilities are limited. Breast cancer, for example, has an incidence of 40% in the upper left, but 20% in the lower right.
암이 발생하는 원인은 아직도 정확히 밝혀진 바가 없지만, 지금까지 알려진 바에 따르면 정상적인 세포의 유전자나 암 억제 유전자에 돌연변이가 생겨서 나타난다고 한다. 대표적인 암 억제 유전자인 p53 유전자의 경우는 자연발생적인 원발성 종양의 약 50%에서 이 유전자의 돌연변이가 관찰되었지만 특정 유전자 몇 개의 변이로만 암이 일어나는 것이 아닌 것은 확실해 보인다. 유전자 치료를 통해 정상 p53유전자를 암세포에 주입했을 경우 환자의 상태가 호전되지 않는다는 연구가 발표되어 있는 것을 봐도 복잡한 원인에 의해서 발생하는 것으로 추정된다. 다만 몇 가지 발암원을 연구함으로써 그것들의 사용을 금지하고 있다. 폐암은 지속적인 흡연(간접 흡연 포함), 도시 공해 등이 발암원이라고 추정되며 간암은 지나친 음주 등이 원인으로 추정된다. 또한 벤젠과 같은 일부 방향족 탄화수소가 강력한 발암원임이 밝혀졌으며 폴리염화비닐을 태울 때 나오는 다이옥신 또한 발암원으로 밝혀져 있다.The cause of cancer is still unknown, but it is known that mutations occur in normal cell genes or cancer suppressor genes. In the case of the p53 gene, a representative cancer suppressor, mutations were observed in about 50% of naturally occurring primary tumors, but it seems clear that only a few mutations in certain genes result in cancer. The study suggests that injection of normal p53 genes into cancer cells through gene therapy does not improve the patient's condition. Only a few carcinogens have been studied to ban their use. It is estimated that lung cancer is a carcinogen due to continuous smoking (including indirect smoking) and urban pollution, and liver cancer is thought to be caused by excessive drinking. Some aromatic hydrocarbons, such as benzene, have also been found to be potent carcinogens, and dioxins from burning polyvinyl chloride have also been identified as carcinogens.
암으로 인해 나타나는 징후와 증상은 암의 종류, 크기와 위치에 따라 다양하다. 암으로 인한 증상과 징후는 암 조직 자체의 영향, 주위의 장기와 구조물에 영향을 줄 때 생기며, 암이 몸의 다른 부위로 전이가 된다면 징후와 증상은 매우 다양하게 나타날 수 있다. 암의 초기 단계에는 특별한 증상이 없는 경우가 많고 증상이 비 특이적이기 때문에 다른 질환과의 구분이 어렵다. 그러나 암이 자라면서 주위의 기관, 구조물, 혈관, 신경을 압박하게 되며 여러 징후와 증상이 나타나게 되는데, 예를 들면 좁은 공간에 있으며 주위에 복잡한 기관이 많은 뇌하수체에 생긴 암 같은 경우는 작은 경우라도 그 증세와 징후가 빨리 나타나지만, 췌장처럼 넓은 복강에 있으며 주위에 복잡한 장기나 기관이 없는 곳에서 생긴 암은 상당히 큰 크기로 자랄 때까지 특별한 증세와 징후가 나타나지 않는 경우도 있다. 또한 암이 피부 가까이에서 커진다면 덩어리로 만져질 수도 있다. 암이 커지면서 나타나는 증상으로는 변비처럼 장기 내강을 막아서 생기는 증세가 있고, 췌장암과 담도암처럼 담관을 막아 황달 등의 징후를 보이기도 한다. 폐암 등은 기관지를 자극하여 기침을 유발하게 된다. 암이 신경이나 혈관을 누르거나, 뼈 등으로 전이가 생긴 경우는 통증을 일으킬 수도 있다. 위암과 대장암처럼 암의 성장으로 조직에서 출혈을 하는 경우 혈변과 빈혈, 폐암은 객혈, 방광암에서는 혈뇨 등이 생기게 된다. 암은 여러 면역기능에도 영향을 줄 뿐 아니라 체중감소, 발열, 피로, 전신쇠약, 식욕저하 등의 전신적인 증세를 만드는데, 이는 암세포에서 만들어진 물질들이 혈관을 통해 전신으로 퍼지며 신체대사에 영향을 주기 때문에 생기는 것이다.The signs and symptoms of cancer vary depending on the type, size and location of the cancer. Cancer symptoms and signs are caused by the effects of cancer tissues themselves and on the surrounding organs and structures. If cancer spreads to other parts of the body, the signs and symptoms can vary greatly. In the early stages of cancer, it is often difficult to distinguish from other diseases because there are often no specific symptoms and the symptoms are nonspecific. However, as the cancer grows, it compresses the surrounding organs, structures, blood vessels, and nerves, causing many signs and symptoms, such as cancers in the pituitary gland in small spaces and many complex organs around them. Symptoms and signs appear quickly, but cancers in the abdominal cavity, such as the pancreas, and where there are no complex organs or organs around them, may not show any special signs and symptoms until they grow to a fairly large size. Also, if the cancer grows near your skin, it can be touched as a lump. As the cancer grows, symptoms such as constipation can be caused by blocking the lumen of organs, and like pancreatic cancer and biliary tract cancer, they block the bile ducts and show signs of jaundice. Lung cancer, etc. will stimulate the bronchus and cause a cough. Cancer can cause pain if it presses on nerves, blood vessels, or metastases to bones. Like cancers of the stomach and colon cancer, bleeding from tissues is caused by the growth of cancer. Cancer not only affects various immune functions, but also causes systemic symptoms such as weight loss, fever, fatigue, general weakness, and loss of appetite, because the substances produced by cancer cells spread through blood vessels throughout the body and affect metabolism. It happens.
암의 치료는 진단된 암의 종류, 진행상태(병기), 환자의 전신상태 등에 따라 결정되며 다른 질환의 치료에 비해 치료방법이 다양하고 복잡하며 부작용이 생길 가능성이 높다. 따라서 치료법의 특징과 장단점을 충분히 이해하는 것이 중요하며, 암의 치료 후 예후를 예측할 수 있는 예후인자는 여러 가지가 있으나 그 중에서도 종양의 크기(T), 주위 림프절로의 전이여부(N), 원격장기로의 전이여부(M)로 결정되는 종양의 진행상태가 가장 중요하며, 암이 많이 진행되었거나 체중감소 등 치료 전 전신상태가 좋지 않은 경우는 예후가 불량한 것으로 알려져 있다. 전이 또는 재발한 암환자에서는 환자의 활동성, 치료 후 무병기간, 수술여부, 암의 특성에 의한 항암제와 방사선 치료의 반응성, 타 장기로의 전이 정도와 전이 부위 등이 예후와 관계가 있으며 이와 같은 임상적 소견 이외에도 환자의 예후와 관계가 있는 병리학적 소견으로는 암세포의 종류, 종양의 크기, 종양의 세포분화도, 유전자 발현 정도 등이 있다. 암환자 치료 후 생존율은 대체로 '5년 생존율'을 말하는데, '5년 생존율'이란 암으로 치료를 받은 환자 중 치료를 시작한 날 부터 5년 이내에 해당 암으로 사망한 환자를 제외한 환자의 비율을 말한다. 이때 '재발하거나 진행하고 있더라도' 생존해 있는 한 생존률에 포함되며, 생존율을 설명할 때 세부적인 구분으로서 '암의 징후가 없는 생존율', '암의 진행이 없는 생존율'로 나눠서 설명하기도 한다.The treatment of cancer is determined by the type of cancer diagnosed, the stage (stage), and the general condition of the patient. The treatment is more diverse, complex, and more likely to cause side effects than the treatment of other diseases. Therefore, it is important to fully understand the characteristics and strengths and weaknesses of the treatment, and there are many prognostic factors that can predict the prognosis after treatment of cancer. Among them, tumor size (T), metastasis to surrounding lymph nodes (N), and distant Tumor progression, which is determined by metastasis to organs (M), is the most important, and the prognosis is known to be poor in cases where the cancer has progressed a lot or the general condition before treatment such as weight loss is poor. In patients with metastasized or relapsed cancer, the prognosis is related to the patient's activity, disease-free period after treatment, surgery, responsiveness to anticancer and radiation therapy due to the characteristics of the cancer, the extent of metastasis to other organs and the site of metastasis. In addition to the pathologic findings, pathological findings related to the patient's prognosis include cancer cell type, tumor size, tumor cell differentiation, and gene expression. Survival rate after treatment of cancer patients generally refers to 'five-year survival rate', and 'five-year survival rate' refers to the proportion of patients treated with cancer excluding those who died of the cancer within five years from the start of treatment. The survival rate is included in the survival rate as long as it survives, even if it recurs or progresses, and it is divided into 'survival rate without cancer' and 'survival rate without cancer' when describing survival rate.
한편, 암 치료와 예방을 놓고 수십 년간 계속되어온 논쟁이 최근 예방에 무게를 실어주는 쪽으로 결론이 나 눈길을 끌고 있다. 스위스 제네바에 있는 국제항암연합(International Union Against Cancer, or IUAC)이 2010년 2월 2일 발표한 보고서에 따르면, 완전한 예방 백신 접종의 실시와 금연, 건강한 식사, 과도의 음주를 피하고, 적정 체중, 운동습관, 자외선 대책 등, 라이프스타일의 개선으로 암의 발병 리스크는 최대 40%나 감소할 수 있다고 한다. 따라서 최근에는 암을 치료하는 측면뿐 아니라 예방하는 면에 더욱 중요성이 증가되고 있다.On the other hand, debates that have been going on for decades over cancer treatment and prevention are drawing attention to recent weight on prevention. According to a report released on February 2, 2010 by the International Union Against Cancer, or IUAC, in Geneva, Switzerland, full vaccinations, smoking cessation, healthy eating, avoiding excessive drinking, Lifestyle improvements, such as exercise habits and UV protection, can reduce the risk of cancer by up to 40 percent. Therefore, in recent years, the importance of preventing as well as treating cancer is increasing.
또한, 현재 사용되고 있는 암 치료제의 경우, 치료 효과가 우수한 치료제는 암 세포 뿐만 아니라 정상 세포도 공격하는 문제점이 있고 환자에게 각종 부작용을 유발시키는 문제점이 발생하고 있다.In addition, in the case of the currently used cancer treatment agent, a therapeutic agent having a superior therapeutic effect has a problem of attacking not only cancer cells but also normal cells and causes various side effects to patients.
따라서 이러한 부작용을 줄이기 위한 노력으로 항암 활성이 우수한 약리 성분을 천연물로부터 수득하려는 연구가 진행되고 있는데, 이러한 연구 결과, 대한민국공개특허 제2010-0066433호에는 해양미생물 추출물을 포함하는 항암 조성물에 대한 내용이 개시되어 있고, 대한민국공개특허 제2010-0062168호에는 느티나무 메탄올 추출물을 포함하는 항암 조성물이 개시되어 있으며, 대한민국공개특허 제2010-0055653호에는 솔이끼 추출물을 유효성분으로 함유하는 항암용 조성물이 개시되어 있는 등 각종 천연물을 이용하여 암을 치료하고자 하는 연구들이 지금도 활발히 진행되고 있다.Therefore, in an effort to reduce such side effects, researches are being conducted to obtain pharmacological components having excellent anticancer activity from natural products. As a result of this study, Korean Patent Publication No. 2010-0066433 discloses an anticancer composition including a marine microbial extract. It is disclosed, the Republic of Korea Patent Publication No. 2010-0062168 discloses an anticancer composition comprising a zelkova methanol extract, the Republic of Korea Patent Publication No. 2010-0055653 discloses an anticancer composition containing the sol moss extract as an active ingredient There are many studies to treat cancer using various natural products.
한편 여주(Momordica charantia L.)는 박과(Cucurbitaceae)에 속하는 채소 또는 약용식물로 주로 열대나 아열대 지역에서 자라며, 주로 열매를 식용으로 이용하는데, 쓴 맛이 강하여 일반적으로 영문으로는 bitter gourd 또는 bitter melon이라고 불린다. 어린 열매는 녹색을 나타내고, 더 성숙되면 오렌지색으로 변하며, 시간이 더 지나면 과육이 터져 빨간색 가종피가 노출되고, 종자는 일반적으로 브라운색을 나타낸다. 일본, 중국, 동남아지역에서는 주로 미숙과를 식용으로 재배하고 있으나 어린잎을 식용으로 이용하기도 한다.Litchi ( Momordica) charantia L.) is a vegetable or medicinal plant belonging to the family Cucurbitaceae. It grows mainly in tropical or subtropical regions, and mainly uses fruit for food. It is called bitter gourd or bitter melon because of its strong bitter taste. Young fruits appear green, turn orange when mature, and over time, the pulp bursts to expose the red apricot, and the seeds generally appear brown. In Japan, China, and Southeast Asia, mainly immature fruits are grown for food, but young leaves are also used for food.
여주는 예로부터 우리나라에서는 집 울타리에 관상용으로 많이 심었는데, 최근에는 미국이나 유럽 등 일부 선진국에서 다양한 건강식품으로 활용되고 있다. 여주의 쓴맛이 위를 자극하여 소화액 분비를 촉진하고 식욕이 생기도록 하고, 건위, 정장 작용도 있다. 또한 동남아시아의 전통 의학에선 피부병, 야맹증, 기생충, 류머티스, 통풍, 신체 허약 등에 효과가 있는 식품으로 여겨 이용해 왔다.Yeoju has been planted a lot on ornamental fences in Korea since ancient times. Recently, it has been used for various health foods in some developed countries such as the United States and Europe. Yeoju's bitter taste stimulates the stomach to promote digestion and appetite, and also has a healthy, formal action. In addition, traditional medicine in Southeast Asia has been used as a food that is effective in skin diseases, night blindness, parasites, rheumatism, gout, and physical weakness.
또한, 여주의 성분 연구는 1960대 이후로 이루어져 왔으며 여러 종류의 일차대사산물과 이차대사산물이 과실, 잎, 줄기, 뿌리, 종자 등에서 분리되어졌다. 여주에는 지방, 조단백질, 수용성 식이섬유, 미네랄, 에센셜 오일, 후라보노이드, 페놀산, 글리코시드, 크리테르펜 등 다양한 일차, 이차대사산물과 특히 미숙과에는 비타민 C와 비타민 A가 풍부한 것으로 나타났다.In addition, research on the composition of Yeoju has been conducted since the 1960s, and various types of primary and secondary metabolites were separated from fruits, leaves, stems, roots, and seeds. In Yeoju, various primary and secondary metabolites such as fats, crude proteins, water soluble fiber, minerals, essential oils, flavonoids, phenolic acids, glycosides and critterpenes, and especially immature fruit, are rich in vitamin C and vitamin A.
여주가 함유하고 있는 이차대사산물(secondary metabolites) 중 cucurbitane-type triterpenoids가 주요 기능성 물질로 알려져 왔으며 그 물질들이 특히 항비만 효과가 있는 것으로 연구되어 왔다. 카란틴(Charantin)은 여주에서 분리된 cucurbitane-type triterpenoid의 일종으로 가장 연구가 많이 이루어진 물질 중에 하나이다. 이 물질은 sitosteryl glucoside와 stigmasteryl glucoside 두 물질이 혼합된 상태로 존재한다고 보고되고 있다.Among the secondary metabolites in Yeoju, cucurbitane-type triterpenoids have been known as major functional substances, and they have been studied to have an anti-obesity effect. Charantin is a type of cucurbitane-type triterpenoid isolated from Yeoju and is one of the most studied. It is reported that this substance exists as a mixture of two substances, sitosteryl glucoside and stigmasteryl glucoside.
또한 여주의 주요 기능성 성분인 카란틴은 인슐린 내성관련 혈당을 낮추는 효과가 높은 것으로 여러 실험을 통해 입증되고 있으며, 또한 여주의 쓴맛에는 식물스테롤 배당체들과 많은 종류의 아미노산, 갈락트론산, 시트룰린, 펙틴 등의 성분이 들어 있어 이 성분들은 혈당강하 기능이 탁월한 것으로 알려져 있고, 특히 식물인슐린이라고도 불리는 펩타이드성 물질인 카란틴(charantin)은 인슐린 분비에 결정적 역할을 하는 베타세포를 활성화시켜 인슐린의 분비를 촉진함으로써 혈당을 낮춰주는 역할을 하는 것으로 보고되고 있다.In addition, carotene, the main functional ingredient of Yeoju, has been proven to have high effect on lowering insulin resistance-related blood sugar, and the bitter taste of Yeoju has phytosterol glycosides and many amino acids, galactronic acid, citrulline, and pectin. These ingredients are known to be excellent in hypoglycemic function, especially charantin, a peptide substance called plant insulin, promotes insulin secretion by activating beta cells that play a crucial role in insulin secretion. It has been reported to play a role in lowering blood sugar.
이와 같이 여주의 생리활성에는 혈압을 낮추는 효과 외에 피를 맑게 해주는 정혈작용(淨血作用), 비타민 C가 많이 함유되어 있어 피부미용 및 변비 해결에도 좋은 효능이 있는 것으로 알려져 있다.As such, the physiological activity of Yeoju is known to have good effects on skin beauty and constipation because it contains a lot of blood clots (정 作用), vitamin C, which clears the blood in addition to the effect of lowering blood pressure.
그러나 아직까지 여주가 암을 예방 또는 개선할 수 있다는 작용에 대해서는 구체적으로 알려진 바 없다. 암은 다양하고 복잡한 기전을 가졌기 때문에 현대의학의 발달에도 불구하고 암은 여전히 치료가 어려운 질병의 하나로 여겨지고 있으며, 암 종류에 따라서는 식생활 패턴이 중요한 요소로 작용하기도 한다. 최근 식품이나 천연물 자원으로부터 암을 예방 또는 치료할 수 있는 식품화학물질(phytochemicals)을 찾으려는 연구들이 다양한 분야에서 활발히 진행되고 있다.However, there is no specific knowledge about the effect that Yeoju can prevent or improve cancer. Because cancer has a variety of complex mechanisms, despite the development of modern medicine, cancer is still considered to be one of the most difficult diseases to treat, and depending on the type of cancer, dietary patterns may play an important role. Recently, researches are actively conducted in various fields to find phytochemicals that can prevent or treat cancer from food or natural product resources.
이에 본 발명자들은 여주가 암을 예방 또는 치료할 수 있는 가능성을 일련의 실험을 통해 일부 확인하였으며, 나아가 안전하고 부작용을 최소화한 여주추출물을 주성분으로 한 항암 조성물을 제조하였다.
Therefore, the present inventors partially confirmed the possibility that Yeoju can prevent or treat cancer through a series of experiments. Furthermore, the present inventors prepared an anti-cancer composition mainly comprising Yeoju extract, which is safe and minimizes side effects.
이에 본 발명자들은 세계 각 품종별 여주로부터 생리활성 성분이 함유된 추출물을 수득하여 각 품종별 항균활성 및 항암활성을 확인하고 여주 추출물이 우수한 항균활성 및 항암활성을 갖는다는 사실을 확인함으로써 본 발명을 완성하였다.The present inventors obtained the present invention by confirming the antimicrobial and anticancer activity of each variety by obtaining the extract containing the physiologically active ingredient from the Yeoju of each variety of the world and confirming the fact that the Yeoju extract has excellent antimicrobial and anticancer activity Completed.
따라서 본 발명의 목적은 암을 효과적으로 예방 또는 개선할 수 있는 여주 추출물을 유효성분으로 포함하는 항암 조성물을 제공하는 것이다.Therefore, it is an object of the present invention to provide an anticancer composition comprising as an active ingredient extracts that can effectively prevent or improve cancer.
또한, 본 발명의 다른 목적은 상기 본 발명에 따른 여주 추출물을 유효성분으로 함유하는 암질환의 예방 또는 개선을 위한 건강기능식품을 제공하는 것이다.
In addition, another object of the present invention to provide a health functional food for the prevention or improvement of cancer diseases containing the extract of the bitter gourd according to the present invention as an active ingredient.
상기와 같은 본 발명의 목적을 달성하기 위해서, 본 발명은 여주 추출물을 유효성분으로 포함하는 항암 조성물을 제공한다.In order to achieve the object of the present invention as described above, the present invention provides an anticancer composition comprising the extract of the bitter gourd as an active ingredient.
본 발명의 일실시예에 있어서, 상기 추출물은 물 또는 유기용매를 이용하여 수득한 것일 수 있다.In one embodiment of the present invention, the extract may be obtained by using water or an organic solvent.
본 발명의 일실시예에 있어서, 상기 유기용매는 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올, 아세톤, 에테르, 벤젠, 클로로포름, 에틸아세테이트 및 메틸렌클로라이드로 이루어진 군 중에서 선택되는 것일 수 있다.In one embodiment of the present invention, the organic solvent may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate and methylene chloride.
본 발명의 일실시예에 있어서, 상기 항암은 간암, 위암, 대장암, 폐암, 유방암, 직장암 및 췌장암으로 이루어진 군 중에서 선택되는 암 질환을 예방 또는 개선할 수 있다.In one embodiment of the present invention, the anti-cancer may prevent or improve cancer disease selected from the group consisting of liver cancer, stomach cancer, colon cancer, lung cancer, breast cancer, rectal cancer and pancreatic cancer.
본 발명의 일실시예에 있어서, 상기 여주 추출물은 암세포의 증식을 억제함을 통해 항암활성을 갖는 것일 수 있다.In one embodiment of the present invention, the bitter extract may have anticancer activity through inhibiting the proliferation of cancer cells.
본 발명의 일실시예에 있어서, 조성물 총 중량에 대하여 상기 여주 추출물을 0.1 내지 50 % 중량으로 포함할 수 있다.In one embodiment of the present invention, the total weight of the composition may include 0.1 to 50% by weight of the extract.
본 발명의 일실시예에 있어서, 상기 여주는 한국 재래종 또는 Peacock, Nikko, Dragon 및 Erabu등으로 이루어진 군 중에서 선택되는 일본 품종의 여주일 수 있다. In one embodiment of the present invention, the bitter gourd may be a bitter gourd of a Japanese variety selected from the group consisting of Korean native species or Peacock, Nikko, Dragon, and Erabu.
또한, 본 발명은 본 발명에 따른 여주 추출물을 유효성분으로 함유하는 암질환의 예방 또는 개선을 위한 건강기능식품을 제공한다.In addition, the present invention provides a health functional food for the prevention or improvement of cancer diseases containing the extract of the bitter gourd according to the present invention as an active ingredient.
본 발명의 일실시예에 있어서, 상기 식품은 분말, 과립, 정제, 캡슐 또는 음료 형태일 수 있다.
In one embodiment of the invention, the food may be in the form of powder, granules, tablets, capsules or beverages.
본 발명에 따른 여주 추출물은 다양한 암세포의 증식을 억제하는 활성이 우수할 뿐만 아니라 인체 유해균에 대한 항균 활성도 우수한 특징이 있으므로 암 질환의 예방 및 치료를 위한 치료제와 더불어 건강기능식품으로 유용하게 사용할 수 있는 효과가 있다.
Yeoju extract according to the present invention is not only excellent in inhibiting the proliferation of various cancer cells, but also has excellent antimicrobial activity against human harmful bacteria, and thus can be usefully used as a health functional food with a therapeutic agent for the prevention and treatment of cancer diseases. It works.
도 1은 본 발명의 일실시예에 따른 여주 추출물의 인간 암세포에 대한 세포독성도를 MTT방법으로 측정하여 나타낸 것이다.Figure 1 shows the cytotoxicity of human extracts of Yeoju in accordance with an embodiment of the present invention measured by MTT method.
본 발명은 여주 추출물을 유효성분으로 포함하는 항암 조성물을 제공함에 그 특징이 있다.The present invention is characterized in that it provides an anticancer composition comprising the extract of the bitter gourd as an active ingredient.
여주(Momordica charantia L.)는 박에 속하는 일년생 덩굴성 초본식물로 미국에서는 bitter melon(bitter gourd), 아프리카에서는 wild cucumber, 인도에서는 karela, 동남아사아에서는 ampalaya 등 다양한 이름으로 불리고 있다. 중국과 일본에서는 약용 식물로서 외상 치료 및 궤양의 치료에 사용되고 있으나(Gurbuz et al.,2000), 우리나라에서는 관상용으로만 재배되어 왔다. 또한, 동남아시아에서는 피부병, 야맹증, 기생충, 류머티스, 통풍, 신체 허약 등에 효과가 있는 식품으로 여겨 이용해 왔고, 중국 명나라 때에는 피로 회복, 정신 안정에 효과가 있어 이러한 용도로 사용되어 왔다.Litchi ( Momordica charantia L.) is an annual herbaceous herbaceous plant belonging to gourds. It is called by various names such as bitter melon (bitter gourd) in America, wild cucumber in Africa, karela in India and ampalaya in Southeast Asia. In China and Japan, it is used as a medicinal plant for the treatment of trauma and ulcers (Gurbuz et al., 2000), but it has been grown only for ornamental purposes in Korea. In addition, Southeast Asia has been used as a food that is effective in skin diseases, night blindness, parasites, rheumatism, gout, physical weakness, etc. In the Chinese Ming Dynasty, it has been used for fatigue recovery and mental stability.
또한, 여주에는 다양한 생리활성 성분이 들어 있는데 글루코시아드, 사포닌, 알카로이드, 오일, 트리페르펜, 단백질, 스테로이드계, 비타민 C, 베타-카로틴, Fe, K 등을 함유하고 있는 것으로 알려져 있고(Oyedapo & Araba, 2001), 특히 비타민C는 100g 중 120mg이나 들어 있어 딸기의 1.5배, 양배추의 3배, 레몬의 약 1.3배에 해당하는 양이 함유되어 있을 뿐 아니라 수분이 많은 과육에 들어 있기 때문에 가열해도 거의 파괴되지 않는 것으로 알려져 있다.In addition, Yeoju contains various bioactive ingredients, which are known to contain glucosiad, saponin, alkaloid, oil, tripperpene, protein, steroid, vitamin C, beta-carotene, Fe, K, etc. (Oyedapo & Araba, 2001), especially vitamin C contains 120mg of 100g, which contains 1.5 times of strawberry, 3 times of cabbage, and about 1.3 times of lemon. It is known that almost no destruction.
한편, 본 발명에서는 여주로부터 수득한 추출물이 암을 예방 및 치료할 수 있다는 사실을 최초로 규명하였다.On the other hand, the present invention was first identified that the extract obtained from Yeoju can prevent and treat cancer.
따라서 본 발명은 여주 추출물을 유효성분으로 함유하는 항암 조성물을 제공할 수 있다.Therefore, the present invention can provide an anticancer composition containing the extract of the bitter gourd as an active ingredient.
본 발명에서 상기 여주는 시중에서 판매되고 있는 여주라면 모두 사용가능하며, 바람직하게는 한국 재래종, 중국 재래종, 일본 품종(예컨대, Peacock, Nikko, Dragon 및 Erabu), 필리핀 품종(예컨대, Verde Buenas, Trident 357, Sta Monica, Galaxy, Sta. Rita Strain. L)을 사용할 수 있으며, 바람직하게는 한국 재래종 또는 일본 품종을 사용할 수 있다.In the present invention, all of the bitter gourd can be used as long as it is commercially available, preferably Korean, Chinese, Japanese varieties (eg, Peacock, Nikko, Dragon and Erabu), Philippine varieties (eg, Verde Buenas, Trident). 357, Sta Monica, Galaxy, Sta. Rita Strain. L), preferably Korean native or Japanese varieties.
본 발명에 따른 상기 여주 추출물은 당업계에 공지된 천연으로부터 추출물을 수득하는 방법이라면 모두 사용할 수 있으며, 바람직하게는 유기용매를 이용한 추출방법을 통해 수득할 수 있다.The bitter gourd extract according to the present invention can be used as long as it is a method for obtaining an extract from nature known in the art, preferably can be obtained through an extraction method using an organic solvent.
본 발명에 따른 여주로부터 항암억제 활성을 갖는 추출물을 얻는 과정을 보다 상세히 살펴보면 다음과 같다.Looking at the process of obtaining an extract with anticancer inhibitory activity from Yeoju according to the present invention in more detail.
먼저, 여주를 얇은 형태로 자른 후, 자른 여주를 여주 과실의 성분이 파괴되지 않도록 저온건조 또는 동결건조하여 건조 중량에 대해 약 1배 내지 10배, 바람직하게는 약 1배 내지 3배의 물 또는 유기용매를 이용하여 당업계에 공지된 용매 추출 방법을 통해 여주의 추출물을 수득할 수 있다.First, the bitter gourd is cut in a thin form, and then the cut bitter gourd is lyophilized or lyophilized so as not to destroy the components of the bitter fruit, and about 1 to 10 times the dry weight, preferably about 1 to 3 times water or The extract of the yeast can be obtained by using an organic solvent through a solvent extraction method known in the art.
또한, 바람직하게 상기 동결건조된 여주는 유기용매를 이용하여 추출하기 이전에 분쇄할 수 있으며, 상기 분쇄는 수작업 또는 기계적 장치를 이용하여 수행할 수 있다.In addition, the freeze-dried litchi may be pulverized prior to extraction using an organic solvent, and the pulverization may be performed by hand or using a mechanical device.
상기 동결건조 단계에서 “동결건조”란 수용액이나 다량의 수분을 함유한 재료를 동결시키고 감압함으로써 얼음을 승화시켜 수분을 제거하여 건조물을 얻는 방법을 말한다. 즉 식품을 동결시키고 온도와 압력이 낮은 3중점(三重點, 0.01℃. 0.6113kPa) 이하에서 동결상태의 얼음을 승화시켜 건조제품을 얻는 방법이다. 본 발명에서 채용하는 동결건조단계를 위하여 사용할 수 있는 동결건조기는 동결건조를 위해 승화열을 공급하는 가열판, 건조 중에 생성된 수증기를 얼음으로 응축시키는 응축기, 진공실 및 진공펌프로 구성되어 있는 건조기라면 특별히 제한되지 않는다.In the freeze-drying step, "freeze-drying" refers to a method of obtaining a dried product by removing water by sublimating ice by freezing and decompressing an aqueous solution or a material containing a large amount of water. In other words, it is a method of freezing foods and subliming frozen ice at a triple point (三 重點, 0.6113kPa) at low temperature and pressure to obtain a dried product. The lyophilizer that can be used for the freeze-drying step employed in the present invention is particularly limited as long as it is a dryer composed of a heating plate for supplying sublimation heat for freeze drying, a condenser for condensing water vapor generated during drying with ice, a vacuum chamber, and a vacuum pump. It doesn't work.
이후 분쇄된 여주는 상기 여주에 유기용매를 첨가하여 교반함으로써 추출물을 수득할 수 있는데, 이때 첨가할 수 있는 상기 유기용매로는 이에 제한되지는 않으나, 메탄올, 에탄올, 프로판올, 이소프로판올 및 n-부탄올 등을 사용할 수 있으며, 상기 용매 추출 방법으로는 이에 제한되지는 않으나, 열수 추출법, 냉침 추출법, 환류 냉각 추출법 또는 초음파 추출법 등을 1회 또는 수회 반복하여 추출할 수 있다. After the crushed bitter gourd can be obtained by adding an organic solvent to the bitter and stirring, the organic solvent can be added, but is not limited to this, methanol, ethanol, propanol, isopropanol and n-butanol, etc. The solvent extraction method may be used, but the present invention is not limited thereto, and may be extracted by repeating the hot water extraction method, the cold needle extraction method, the reflux cooling extraction method, or the ultrasonic extraction method once or several times.
본 발명의 일실시예에서는 여주를 동결건조한 후 분쇄한 다음, 99.8% 메탄올을 첨가하여 상온에서 24시간 3회 추출하였다.In one embodiment of the present invention lyophilized after lyophilization and pulverized, it was extracted three times at room temperature by adding 99.8% methanol.
이후 추출된 여주 추출물은 감압, 여과 및 농축 과정을 거쳐 각종 유기용매에 용해되어 농축된 여주 추출물의 형태로 제조할 수 있다.Then, the extracted Yeoju extract can be prepared in the form of a concentrated Yeoju extract dissolved in various organic solvents through a process under reduced pressure, filtration and concentration.
한편, 일반적으로 암은 발암물질에 의해 DNA에 돌연변이가 유도되는 단계, 양성 종양(benign tumor)이 나타나는 단계, 양성종양이 악성종양(malignant tumor)으로 변환되는 단계 및 악성 정도의 증가와 전이로의 단계와 같이 다단계에 의해 진행되는 것으로 알려져 있으며, 이러한 암의 치료는 암세포의 세포증식을 억제하거나 또는 암세포의 세포사멸을 유도하는 방법들이 사용되고 있다.On the other hand, in general, cancer is a step in which mutations are induced in DNA by carcinogens, a benign tumor appears, a benign tumor is converted into a malignant tumor, and an increase in the degree of malignancy and metastasis It is known to proceed by a multi-step as step, and the treatment of such cancer is used to inhibit the cell proliferation of cancer cells or to induce apoptosis of cancer cells.
이에 본 발명자들은 상기 본 발명의 여주 추출물이 항암 활성이 있는지를 조사하였는데, 즉, 본 발명의 일실시예에 따르면, 본 발명에서 수득한 각 품종별 여주 추출물을 인간유래 암세포주인 위암 세포 SNU-601, 직장암 세포 HCT 116, 대장암 세포 Caco-2, 유방암 세포 MCF-75에 처리한 다음, MTT 분석을 통해 세포의 생존율을 측정하였다. Therefore, the present inventors examined whether the extract of the present invention has anticancer activity, that is, according to an embodiment of the present invention, the extract of each strain obtained in the present invention is gastric cancer cell SNU-601 which is a human-derived cancer cell line. , HCT 116, colorectal cancer cells Caco-2, breast cancer cells MCF-75, and then treated with MTT assay, the survival rate of the cells was measured.
그 결과, 각 여주 품종들 모두 인체 암세포에 대한 세포 독성도를 보였고, 이러한 세포 독성을 통해 암 세포의 증식을 억제하는 활성이 있는 것으로 나타났으며, 특히, 한국 재래종이 전반적으로 암세포에 대해 높은 독성을 보여 가장 좋은 항암활성을 갖는다는 것을 알 수 있었다(표 2 참조).As a result, each of the varieties of yeast showed cytotoxicity against human cancer cells, and the cytotoxicity was shown to inhibit the proliferation of cancer cells. Especially, Korean native species showed high toxicity against cancer cells. Showed the best anticancer activity (see Table 2).
따라서 상기 결과를 통해 본 발명자들은 본 발명에 따른 각 품종별 여주 추출물이 암 세포의 세포증식을 억제함으로써 항암 활성을 갖는 사실을 확인하였다.Therefore, through the above results, the present inventors confirmed that the extracts of each strain according to the present invention had anticancer activity by inhibiting cell proliferation of cancer cells.
나아가, 본 발명에 따른 여주 분말의 추출물은 항균 활성이 우수한 특징이 있다.Furthermore, the extract of the bitter gourd powder according to the present invention has excellent antibacterial activity.
최근 건강과 장수에 대한 관심이 증대됨에 따라 안전한 먹거리 확보에 대한 관심이 고조되고 있기 때문에, 유용 생리 활성을 가지면서 부작용이 없는 천연물의 활성물질 탐색에 연구가 집중되고 있으며, 특히 인체에 유해한 세균들에 대한 항균활성을 가진 천연물의 경우 보다 안전하게 사용할 수 있다.Recently, as interest in health and longevity has increased, there has been a growing interest in securing safe foods. Therefore, research has been focused on searching for active substances of natural products having useful physiological activities and no side effects, especially harmful bacteria. Natural products with antimicrobial activity against can be used more safely.
이에 본 발명자들은 본 발명에서 제조한 여주 추출물이 항균 활성이 있는지를 조사하기 위해, 품종별 여주 추출물 처리에 따른 항균력을 한국미생물은행으로부터 식품유해균을 분양받아 agar diffusion test를 통해 저해환의 생성을 확인하였다.In order to investigate whether the Yeoju extract prepared in the present invention has an antimicrobial activity, the antimicrobial activity of the varieties of Yeoju extract was treated with food harmful bacteria from the Korea Microbial Bank and confirmed the production of inhibitory rings through the agar diffusion test. .
그 결과, 그람 양성균 및 그람 음성균 10종의 식품유해균 중에서 Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Enterococcus hirae, Vibrio parahaemdyticus에 대하여 저해환을 형성하여 우수한 항균활성을 갖는 것으로 나타났고, 특히, 그람 음성균인 S. typhimurium에 대해서는 가장 높은 항균활성을 보였다.As a result, Staphylococcus aureus and Bacillus among 10 gram-positive and 10 gram-negative bacteria subtilis , Micrococcus luteus , Enterococcus hirae , Vibrio Inhibition of parahaemdyticus formed an excellent antimicrobial activity, and showed the highest antimicrobial activity, especially against S. typhimurium Gram-negative bacteria.
따라서 본 발명에 따른 여주 추출물은 인체 유해균에 대해 우수한 항균 활성을 가지고 있으므로 보다 안전하게 약학적 용도 및 식품학적 용도로 사용할 수 있다.Therefore, Yeoju extract according to the present invention has excellent antimicrobial activity against human harmful bacteria can be used more safely in pharmaceutical and food use.
그러므로 본 발명은 여주 추출물을 유효성분으로 포함하는 항암 조성물을 제공할 수 있으며, 상기 본 발명의 조성물은 암의 예방 및 치료를 위한 약학적 조성물로 사용될 수 있고, 여주 추출물을 유효성분으로 함유하는 조성물을 포함하는 암 증상의 예방 또는 치료를 위한 약제를 제공할 수 있다.Therefore, the present invention can provide an anticancer composition comprising the extract of Yeoju as an active ingredient, the composition of the present invention can be used as a pharmaceutical composition for the prevention and treatment of cancer, the composition containing the extract of Yeoju as an active ingredient It can provide a medicament for the prevention or treatment of cancer symptoms comprising.
본 발명에 따른 상기 조성물에 있어서, 여주 추출물은 조성물 총 중량에 대해 0.1 내지 50 중량%로 포함할 수 있다.In the composition according to the present invention, the extract extract may include 0.1 to 50% by weight relative to the total weight of the composition.
또한, 본 발명의 조성물이 예방 또는 치료할 수 있는 상기 암의 종류로는 이에 제한되지는 않으나, 간암, 위암, 대장암, 폐암, 유방암, 직장암 및 췌장암일 수 있으며, 바람직하게는 대장암 및 유방암일 수 있다.In addition, the kind of the cancer which the composition of the present invention can prevent or treat is not limited thereto, and may be liver cancer, stomach cancer, colon cancer, lung cancer, breast cancer, rectal cancer and pancreatic cancer, preferably colon cancer and breast cancer. Can be.
본 발명에 따른 상기 항암 조성물은 약학적으로 유효한 양의 여주 추출물을 단독으로 포함하거나 하나 이상의 약학적으로 허용되는 담체, 부형제 또는 희석제를 포함할 수 있다. 상기에서 "약학적으로 유효한 양"이란 암 증상을 예방 및 치료하기에 충분한 양을 말한다.The anticancer composition according to the present invention may include a pharmaceutically effective amount of bitter gourd extract alone or may include one or more pharmaceutically acceptable carriers, excipients or diluents. As used herein, "pharmaceutically effective amount" refers to an amount sufficient to prevent and treat cancer symptoms.
본 발명에 따른 여주 추출물의 약학적으로 유효한 양은 0.5 ~ 100 mg/day/체중kg, 바람직하게는 0.5 ~ 5 mg/day/체중kg이다. 그러나 상기 약학적으로 유효한 양은 암 증상의 정도, 환자의 연령, 체중, 건강상태, 성별, 투여 경로 및 치료기간 등에 따라 적절히 변화될 수 있다.Pharmaceutically effective amount of Yeoju extract according to the present invention is 0.5 ~ 100 mg / day / kg body weight, preferably 0.5 ~ 5 mg / day / kg body weight. However, the pharmaceutically effective amount may be appropriately changed depending on the extent of cancer symptoms, the age, weight, health condition, sex, route of administration and duration of treatment of the patient.
또한, 상기에서 "약학적으로 허용되는"이란 생리학적으로 허용되고 인간에게 투여될 때, 통상적으로 위장 장애, 현기증과 같은 알레르기 반응 또는 이와 유사한 반응을 일으키지 않는 조성물을 말한다. 상기 담체, 부형제 및 희석제의 예로는, 락토즈, 덱스트로즈, 수크로즈, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 폴리비닐피롤리돈, 물, 메틸하이드록시벤조에이트, 프로필하이드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유를 들 수 있다. 또한, 충진제, 항응집제, 윤활제, 습윤제, 향료, 유화제 및 방부제 등을 추가로 포함할 수 있다. In addition, "pharmaceutically acceptable" as used herein refers to a composition that is physiologically acceptable and does not normally cause an allergic reaction, such as gastrointestinal disorders, dizziness, or the like when administered to a human. Examples of such carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In addition, fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers and preservatives may be further included.
또한, 본 발명의 조성물은 포유동물에 투여된 후 활성 성분의 신속, 지속 또는 지연된 방출을 제공할 수 있도록 당업계에 공지된 방법을 사용하여 제형화될 수 있다. 제형은 분말, 과립, 정제, 에멀젼, 시럽, 에어로졸, 연질 또는 경질 젤라틴 캅셀, 멸균 주사용액, 멸균 분말의 형태일 수 있다.In addition, the compositions of the present invention may be formulated using methods known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal. The formulations may be in the form of powders, granules, tablets, emulsions, syrups, aerosols, soft or hard gelatine capsules, sterile injectable solutions, sterile powders.
본 발명에 따른 항암 조성물은 경구, 경피, 피하, 정맥 또는 근육을 포함한 여러 경로를 통해 투여될 수 있으며, 활성 성분의 투여량은 투여 경로, 환자의 연령, 성별, 체중 및 환자의 중증도 등의 여러 인자에 따라 적절히 선택될 수 있다. 또한, 본 발명의 항암 조성물은 암 증상을 예방 또는 개선하는 효과를 가지는 공지의 화합물과 병행하여 투여할 수 있다.The anticancer composition according to the present invention may be administered through various routes including oral, transdermal, subcutaneous, intravenous or intramuscular, and the dosage of the active ingredient is determined by various factors such as the route of administration, the age, sex, weight and severity of the patient. It may be appropriately selected depending on the factor. In addition, the anticancer composition of the present invention can be administered in parallel with a known compound having the effect of preventing or ameliorating cancer symptoms.
나아가 본 발명에 따른 여주 추출물은 우수한 항암 효과를 통해 암 증상을 완화시키는 효과를 제공할 뿐만 아니라, 독성 및 부작용도 없어 장기간 복용시에도 안심하고 사용할 수 있으며, 본 발명에서 사용한 추출물의 원료가 되는 여주는 식품 등급으로 사용할 수 있는 천연식물이므로, 상기 본 발명의 조성물은 체내에 대해 매우 안정한 특징이 있다.Furthermore, Yeoju extract according to the present invention not only provides the effect of relieving cancer symptoms through excellent anti-cancer effect, but also has no toxicity and side effects, so it can be used with confidence even in long-term use, and Yeoju which is a raw material of the extract used in the present invention Since is a natural plant that can be used in food grade, the composition of the present invention has a very stable characteristics for the body.
또한, 본 발명의 여주 추출물은 인체 유해군을 저해하는 활성 및 항암 활성이 우수하므로 암 증상의 예방 및 개선을 목적으로 하는 식품에 첨가할 수 있으므로, 상기 본 발명의 여주 추출물을 포함하는 조성물은 암 증상의 예방 및 개선을 위한 식품용 조성물로도 사용할 수 있다.In addition, the extract extract of the present invention is excellent in the inhibitory activity and anti-cancer activity of human harmful groups, so it can be added to food for the purpose of preventing and improving cancer symptoms, the composition comprising the extract of the present invention is cancer It can also be used as a food composition for the prevention and improvement of symptoms.
그러므로 본 발명에 따른 여주 추출물은 암 증상의 예방 및 개선에 효과가 있는 식품, 예컨대, 식품의 주원료, 부원료, 식품 첨가제, 기능성 식품 또는 음료의 제조에 용이하게 활용할 수 있다.Therefore, extract extract according to the present invention can be easily used for the production of foods, such as the main ingredients, secondary ingredients, food additives, functional foods or beverages that are effective in preventing and improving cancer symptoms.
본원에서 상기 “식품”이란, 영양소를 한 가지 또는 그 이상 함유하고 있는 천연물 또는 가공품을 의미하며, 바람직하게는 어느 정도의 가공 공정을 거쳐 직접 먹을 수 있는 상태가 된 것을 의미하며, 통상적인 의미로서, 식품, 식품 첨가제, 기능성 식품 및 음료를 모두 포함하는 것을 말한다.As used herein, the term “food” refers to a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through a certain processing step, It includes all foods, food additives, functional foods and drinks.
본원발명에 따른 여주 추출물을 포함하는 조성물을 첨가할 수 있는 식품으로는 예를 들어, 각종 식품류, 음료, 껌, 차, 비타민 복합제, 기능성 식품 등이 있다. 추가로, 본원발명에서 식품에는 특수영양식품(예, 조제유류, 영,유아식 등), 식육가공품, 어육제품, 두부류, 묵류, 면류(예, 라면류, 국수류 등), 빵류, 건강보조식품, 조미식품(예, 간장, 된장, 고추장, 혼합장 등), 소스류, 과자류(예, 스넥류), 캔디류, 쵸코렛류, 껌류, 아이스크림류, 유가공품(예, 발효유, 치즈 등), 기타 가공식품, 김치, 절임식품(각종 김치류, 장아찌 등), 음료(예, 과실 음료, 채소류 음료, 두유류, 발효음료류 등), 천연조미료(예, 라면 스프 등)를 포함하나 이에 한정되지 않는다. 상기 식품, 음료 또는 식품첨가제는 통상의 제조방법으로 제조될 수 있다.Examples of the food to which the composition containing the extract containing the extract according to the present invention may be added include various foods, beverages, gums, teas, vitamin complexes, and functional foods. In addition, in the present invention, food includes special nutritional products (e.g., formulated milk, young, infant food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), bread, health supplements, seasonings. Foods (e.g. soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), candy, chocolates, gums, ice creams, dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, Pickled foods (various kimchi, pickles, etc.), beverages (e.g., fruit drinks, vegetable drinks, soy milk, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.) are not limited thereto. The food, beverage or food additive may be prepared by a conventional production method.
또한, 상기 “기능성 식품”이란 식품에 물리적, 생화학적, 생물공학적 수법 등을 이용하여 해당 식품의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 식품군이나 식품 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체내조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 식품을 의미하며, 구체적으로는 건강 기능성 식품일 수 있다. 상기 기능성 식품에는 식품학적으로 허용 가능한 식품 보조 첨가제를 포함할 수 있으며, 기능성 식품의 제조에 통상적으로 사용되는 적절한 담체, 부형제 및 희석제를 더욱 포함할 수 있다.In addition, the term "functional food" refers to the control of biological defense rhythms and disease prevention of food groups or food compositions that have added value to the food by using physical, biochemical, or biotechnological techniques to act and express the function of the food for a specific purpose. It means a food that is designed and processed to fully express the body's regulatory function regarding recovery and the like, and specifically, it may be a health functional food. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.
또한, 본원발명에서 상기“음료”란 갈증을 해소하거나 맛을 즐기기 위하여 마시는 것의 총칭을 의미하며 기능성 음료를 포함한다. 상기 음료는 지시된 비율로 필수 성분으로서 상기 식품유해군의 저해 또는 암 증상의 예방 및 개선용 조성물을 포함하는 것 외에 다른 성분에는 특별한 제한이 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.In addition, in the present invention, the "beverage" refers to a generic term for drinking to quench thirst or to enjoy a taste and includes a functional drink. The beverage is an essential ingredient in the ratio indicated, and includes no composition other than the composition for preventing and improving the inhibition or cancer symptoms of the harmful food group, and various flavors or natural carbohydrates, such as ordinary drinks May be contained as an additional component.
나아가 상기 기술한 것 이외에 본원발명의 조성물을 함유하는 식품은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 충진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있으며, 상기 성분은 독립적으로 또는 조합하여 사용할 수 있다.Furthermore, the food containing the composition of the present invention in addition to the above-mentioned composition can be used as a food such as various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers (cheese, And carbonates used in alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonated drinks, etc., and these components may be used independently or in combination Can be used.
또한, 본 발명에 따른 식품용 조성물을 함유하는 식품에 있어서, 상기 본 발명에 따른 조성물의 양은 전체 식품 중량의 0.001중량% 내지 90중량%로 포함할 수 있으며, 바람직하게는 0.1 중량% 내지 40 중량%로 포함할 수 있고, 음료의 경우, 100ml를 기준으로 0.001g 내지 2g, 바람직하게는 0.01g 내지 0.1g의 비율로 포함할 수 있으나, 건강 및 위생을 목적으로 하거나 건강 조절을 목적으로 하는 장기간 섭취의 경우에는 상기 범위 이하일 수 있으며, 유효성분은 안전성 면에서 아무런 문제가 없기 때문에 상기 범위 이상의 양으로 사용될 수 있으므로 상기 범위에 한정되는 것은 아니다.In addition, in the food containing the composition for food according to the present invention, the amount of the composition according to the present invention may comprise from 0.001% to 90% by weight of the total food weight, preferably 0.1% to 40% by weight %, And in the case of a beverage, it may be included in a ratio of 0.001g to 2g, preferably 0.01g to 0.1g based on 100ml, but for a long time for the purpose of health and hygiene or for health control In the case of ingestion may be less than the above range, the active ingredient is not limited to the above range because it may be used in an amount above the above range because there is no problem in terms of safety.
그러므로 본 발명은 여주 추출물을 유효성분으로 함유하는 암 질환의 예방 및 개선을 위한 건강기능식품을 제공할 수 있으며, 상기 식품의 형태는 이에 제한되지는 않으나, 분말, 과립, 정제, 캡슐 또는 음료 형태일 수 있다.
Therefore, the present invention can provide a health functional food for the prevention and improvement of cancer diseases containing the extract of Yeoju as an active ingredient, the form of the food is not limited to this, powder, granule, tablet, capsule or beverage form Can be.
이하 본 발명을 실시예에 의하여 더욱 상세하게 설명한다. 이들 실시예는 단지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 국한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.
Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.
<< 실시예Example 1> 1>
여주 추출물의 제조Preparation of Yew Extract
여주는 국가별, 품종별 여주를 사용하였다. 사용한 품종별 여주의 종류로는 한국품종은 재래종을 사용하였고, 일본품종은 재래종인 Peacock, Nikko, Dragon 및 Erabu 를 사용하였으며, 필리핀품종은 Verde Buenas, Trident357, Sta Monica, Galaxy, Sta. Rita Strain. L을 사용하였다. 이와 같은 여주를 이용하여 여주 추출물을 수득하는 방법은 먼저 친환경 유기적 방법으로 재배된 여주를 수확한 후, 상기 여주 중 적당한 과실을 선별하고, 선별해 낸 여주 과실을 세척하였다. 세척된 여주를 종자를 함유한 상태로 얇은 조각으로 잘게 슬라이스 형태로 절단한 후, 동결건조 하였다. 건조된 시료를 곱게 마쇄하여 분말화한 후, 분말시료와 유기용매(99.8% methanol)를 1:5 비율로 첨가하여 상온에서 24시간 3회 반복 추출한다. 추출액은 감압증발 농축기를 사용하여 1/10로 농축한 후 동결건조하여 시료로 사용한다.
Yeoju was used by country and varieties. Korean varieties were Korean varieties and Korean varieties Peacock, Nikko, Dragon and Erabu were used as varieties. The Philippine varieties were Verde Buenas, Trident357, Sta Monica, Galaxy, Sta. Rita Strain. L was used. In the method of obtaining the extract of the host by using such a host, firstly harvesting the host cultivated by an environmentally friendly organic method, and then selecting the appropriate fruit of the host and washing the selected host fruit. The washed bitter gourd was cut into fine pieces into thin pieces containing seeds, and lyophilized. The dried sample is finely ground and powdered, and then the powder sample and the organic solvent (99.8% methanol) are added at a ratio of 1: 5 and extracted three times at room temperature for 24 hours. The extract is concentrated to 1/10 using a reduced pressure evaporator and lyophilized to use as a sample.
<< 실험예Experimental Example 1> 1>
여주 품종별 추출물의 식품유해균에 대한 항균활성 검색Screening of Antimicrobial Activity of Food-Contracting Bacteria from Extracts of Yeoju Varieties
여주 품종별 추출물의 식품유해균에 대한 항균활성을 측정하기 위해 균주는 한국미생물은행에서 분양받은 식품유해균 11종을 사용하고, Bactotrypton (10g/L), Yeast Extract (5g/L), NaCl (10g/L)이 함유된 배지 (pH.7.0)에서 배양하였으며 각 품종별로 채취한 여주 추출물의 항균력은 agar diffusion test를 사용하여 측정하였다. 즉, 각 시험미생물에 적합한 배지에 107 CFU/ml 농도로 시험미생물을 배양한 soft agar를 petri dish에 분주한 후, 여주 추출물 40㎕를 분주한 paper disc를 올려놓고 배양하여 저해환의 생성을 관찰하였다. 24시간 이내에 저해환이 생성된 경우 항균활성이 양성인 것으로 판정하였으며, 저해환의 직경을 측정, 비교하였다. 항균활성 측정을 위한 균주의 배양조건을 하기 표 1에 기재하였다.In order to measure the antimicrobial activity of food extracts of Yeoju varieties, strains were used 11 kinds of food harmful bacteria, which were distributed by Korea Microbial Bank, Bactotrypton (10g / L), Yeast Extract (5g / L), NaCl (10g / The antimicrobial activity of Yeoju extracts collected in each variety was measured using the agar diffusion test. That is, after dispensing the soft agar cultured the test microorganism at a concentration of 10 7 CFU / ml in a petri dish in a medium suitable for each test microorganism in a petri dish, put the paper disc with 40 μl of Yeoju extract and incubated to observe the formation of inhibitory ring It was. When the inhibitory ring was formed within 24 hours, it was determined that the antimicrobial activity was positive, and the diameter of the inhibiting ring was measured and compared. Culture conditions of the strain for the measurement of antimicrobial activity are described in Table 1 below.
그 결과, 도 1에 나타낸 바와 같이, 그람양성균 및 그람음성균 10종의 식품유해균 중에서 Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Enterococcus hirae, Vibrio parahaemdyticus 등에 대하여 13~17mm의 저해환을 형성하여 항균활성이 높은 것으로 나타났다. 특히, 그람 음성균인 S. typhimurium에 대해서 가장 높은 항균활성을 보였다.
As a result, as shown in Fig. 1, Staphylococcus aureus , Bacillus among 10 gram-positive bacteria and gram-negative bacteria subtilis , Micrococcus luteus , Enterococcus hirae , Vibrio The antibacterial activity of parahaemdyticus was increased by forming an inhibitory ring of 13 ~ 17mm. In particular, it showed the highest antimicrobial activity against S. typhimurium , a Gram-negative bacterium.
<< 실험예Experimental Example 2> 2>
여주 추출물의 각종 인체 암세포에 대한 증식억제 효과 분석Analysis of Growth Inhibitory Effects of Yeoju Extracts on Various Human Cancer Cells
각종 인체 암세포에 대한 증식억제 효과 측정을 위한 여주 추출물을 조제하기 위해 각 처리별로 제조한 시료를 동결건조(Freeze dryer, samwon Co.)하여 마쇄한 후, 99.8% 메탄올을 건물양의 5배 정도 첨가하여 상온에서 24시간 3회 추출하였다, 추출액은 감압증발 농축기를 사용하여 1/10로 농축한 후 동결건조하여 분말화하여 시료로 사용하였다. 또한 분획물 제조를 위해 상기 와 동일한 방법으로 동결건조한 시료로 메탄올 추출물을 조제한 후, 이 추출물을 각 용매별로 분획하여 hexane층, ethylacetate층, butanol층 및 물 층으로 분획하고 각 분획층을 감압농축한 다음 동결건조한 시료를 분석에 사용하였다. 사용한 세포는 인간유래 암세포주로서 위암 세포인 SNU-601, 직장암 세포인 HCT 116, 대장암 세포인 Caco-2, 유방암 세포인 MCF-75등을 사용하였다. SNU-601은 RPMI 1640 복합배지를, HCT 116은 DMEM(Delbecco's Modified Eagle Medium)을 이용하였고 10% Fetal Bovine Serum과 항생제(Antibiotic antimycotic)를 첨가하여 37℃, 5% CO2의 습윤화된 인큐베이터에서 적응시켜 배양하였다. 세포는 dish의 80% 정도 자랐을 때 PBS로 세척하여 Trypsin-EDTA (GibCo/BRL)을 처리하여 2-3일마다 계대 배양하였다. 그리고 그 효과를 분석하기 위한 방법으로, MTT 방법을 약간 변형하여 3번 반복하여 세포 독성도를 측정하였다. 96-웰 마이크로플레이트(Falcon, USA)의 각 웰에 대수기에 도달한 인체 암세포주를 3 X 104 cells/ml의 농도가 되도록 조절하여 세포 부유액을 웰 당 90㎕씩 분주하여 24시간동안 배양하였다. 각각의 시료는 농도별로 10ml씩 첨가하고, 이 때 약물대신 phosphate buffered saline(PBS)을 첨가한 것을 세포의 대조군으로 하였으며, 세포 대신 배양액만을 넣어 blank로 삼았다. CO2 배양기 내에서 3일간 배양 후 모든 웰에 MTT용액(5mg/ml PBS, Sigma, USA) 10㎕씩 가해주고 다시 37℃, 5% CO2의 습윤 배양기에서 4시간 동안 반응하여 MTT가 환원되도록 하였다. 각 웰에 생성된 formazan 결정을 DMSO 150㎕로 잘 녹여서 microplate reader(Bio-Rad, USA)를 이용하여 540nm에서 흡광도를 측정하여 측정된 흡광도로부터 하기의 계산식으로 생존한 세포의 비율을 구하였다.
In order to prepare Yeoju extract for measuring proliferation inhibitory effect on various human cancer cells, samples prepared by each treatment were lyophilized (Freeze dryer, samwon Co.), and 99.8% methanol was added about 5 times the dry weight. The extract was extracted three times at room temperature for 24 hours. The extract was concentrated to 1/10 using a reduced pressure evaporator, and then lyophilized to powder to use. In addition, methanol extract was prepared by lyophilizing the sample in the same manner as the above to prepare the fraction, and then the extract was fractionated by each solvent, and then fractionated into hexane layer, ethylacetate layer, butanol layer and water layer, and each fraction layer was concentrated under reduced pressure. Lyophilized samples were used for analysis. The cells used were human-derived cancer cell lines such as SNU-601, gastric cancer cells, HCT 116, rectal cancer cells, Caco-2, colon cancer cells, and MCF-75, breast cancer cells. SNU-601 was mixed with RPMI 1640 medium, HCT 116 was using DMEM (Delbecco's Modified Eagle Medium), and 10% Fetal Bovine Serum and Antibiotic (antibiotic antimycotic) were added in a humidified incubator at 37 ° C and 5% CO 2 . Adapted and incubated. Cells were washed with PBS when 80% of the dish was grown and treated with Trypsin-EDTA (GibCo / BRL) and passaged every 2-3 days. And as a method for analyzing the effect, a slight modification of the MTT method was repeated three times to measure the cytotoxicity. A human cancer cell line reaching the log phase in each well of a 96-well microplate (Falcon, USA) was adjusted to a concentration of 3 × 10 4 cells / ml, and the cell suspension was dispensed at 90 μl / well for 24 hours. It was. Each sample was added 10ml by concentration, at which time phosphate buffered saline (PBS) was added instead of the drug as a control of the cells, and the culture medium instead of cells were used as blank. After 3 days of incubation in a CO 2 incubator, 10 μl of MTT solution (5mg / ml PBS, Sigma, USA) was added to all wells, and then reacted for 4 hours in a wet incubator at 37 ° C. and 5% CO 2 to reduce MTT. It was. Formazan crystals produced in each well were dissolved in 150 μl of DMSO, and the absorbance was measured at 540 nm using a microplate reader (Bio-Rad, USA) to determine the percentage of cells that survived from the measured absorbance.
<계산식><Calculation Formula>
Percent of viable cells(%) = {(optical density with cytotoxic drug)/(optical density without cytotoxic drug)} X 100
Percent of viable cells (%) = {(optical density with cytotoxic drug) / (optical density without cytotoxic drug)} X 100
그 결과, 상기 표 2에 나타낸 바와 같이, MTT방법에 의한 여주 추출물의 인체 암세포에 대한 세포독성도는 대장암세포에서는 일본 품종 ‘Peacock’과 ‘Nikko’에서 가장 좋은 효과가 있는 것으로 나타났으며, 직장암세포에 대해서는 한국 재래종이 가장 좋은 결과를 보였고, 위암세포 및 유방암세포에 대해서도 한국 재래종과 일본 품종 ‘Nikko’가 상대적으로 높은 효과를 나타냈다.As a result, as shown in Table 2, the cytotoxicity of Yeoju extract by MTT method on human cancer cells was found to be the most effective in the Japanese varieties 'Peacock' and 'Nikko' in colorectal cancer cells, rectal cancer cells Korean native species showed the best results, and Korean native species and Japanese varieties 'Nikko' showed relatively high effects on gastric cancer cells and breast cancer cells.
또한 상기 표 3에 나타낸 바와 같이, 한국 재래종과 일본품종 ‘Nikko’를 시료로 각 용매분획별 추출물의 인체암세포에 대한 세포독성도를 분석한 결과, ethylacetate(EtOAc) 분획층에서 상대적으로 가장 효과가 높았으며, 다음으로 butanol(BuOH)분획층, hexane분획층 순으로 높게 나타났다. 또한 물 분획층도 본 실험에서 처리한 농도에서는 거의 효과가 나타나지 않았으나 더 높은 농도로 처리했을 때는 일부 효과가 있을 것으로 추측 가능 하였다.In addition, as shown in Table 3, as a result of analyzing the cytotoxicity of human cancer cells of each solvent fraction with the sample of Korean native and Japanese varieties 'Nikko', the ethylacetate (EtOAc) fractionation layer was the most effective. Next, butanol (BuOH) fractionation layer, hexane fractionation layer was the highest. In addition, the water fractionation layer showed little effect at the concentration treated in this experiment, but it could be assumed that some effects would be obtained when the concentration was higher.
이와 같이 분획별 추출물에 따른 항암활성은 본 실험에 공시한 모든 암세포주에서 유사한 경향을 보였다.As described above, the anticancer activity according to the fractional extract showed similar tendency in all the cancer cell lines disclosed in this experiment.
따라서 상기 결과를 통해 본 발명에 따른 여주 추출물은 직장암, 위암, 대장암, 유방암 세포의 세포증식을 억제함을 통해 항암활성을 갖는다는 사실을 알 수 있었으며, 특히 대장암 및 유방암에 그 효과가 탁월하다는 사실을 알 수 있었다.
Therefore, it can be seen from the above results that the Yeoju extract according to the present invention has anticancer activity by inhibiting cell proliferation of rectal cancer, stomach cancer, colon cancer and breast cancer cells, and particularly, the effect is excellent for colon cancer and breast cancer. I could see that.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.
So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.
Claims (4)
상기 추출물은 한국 재래종 또는 Peacock, Nikko, Dragon 및 Erabu로 이루어진 군 중에서 선택되는 일본 품종의 여주를 물, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올, 아세톤, 에테르, 벤젠, 클로로포름, 에틸아세테이트 및 메틸렌클로라이드로 이루어진 군 중에서 선택되는 용매로 추출한 것을 특징으로 하는 항암 조성물.The method of claim 1,
The extract is composed of Korean native species or Japanese varieties selected from the group consisting of Peacock, Nikko, Dragon and Erabu with water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate and methylene chloride. An anticancer composition, characterized in that extracted with a solvent selected from the group consisting of.
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| JP2002205953A (en) * | 2001-01-10 | 2002-07-23 | Asahi Denka Kogyo Kk | Antitumor composition |
| JP2007190004A (en) | 2006-01-16 | 2007-08-02 | Shinichi Shioda | Fucoidan-containing health supplementary food |
| KR20080031049A (en) * | 2005-07-08 | 2008-04-07 | 바이오겐 아이덱 엠에이 인코포레이티드 | ANTI-alpha;nu;beta;6 ANTIBODIES AND USES THEREOF |
| KR20090027241A (en) * | 2006-07-10 | 2009-03-16 | 바이오겐 아이덱 엠에이 인코포레이티드 | Compositions and methods for inhibiting growth of smad4-deficient cancers |
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| KR20080031049A (en) * | 2005-07-08 | 2008-04-07 | 바이오겐 아이덱 엠에이 인코포레이티드 | ANTI-alpha;nu;beta;6 ANTIBODIES AND USES THEREOF |
| JP2007190004A (en) | 2006-01-16 | 2007-08-02 | Shinichi Shioda | Fucoidan-containing health supplementary food |
| KR20090027241A (en) * | 2006-07-10 | 2009-03-16 | 바이오겐 아이덱 엠에이 인코포레이티드 | Compositions and methods for inhibiting growth of smad4-deficient cancers |
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