JPH08239304A - Antibacterial agent - Google Patents

Abstract

PURPOSE: To obtain a biguanide-based antibacterial agent absolutely free from fungi in the agent and capable of preventing the secondary contamination of a food factory, etc., with fungus by adding a fungicide to a biguanide-based antibacterial agent. CONSTITUTION: This biguanide-based antibacterial agent contains a fungicide (especially preferably a halogen-containing nitrogen-sulfur compound or an isothiazoline compound). The biguanide-based antibacterial agent is chlorhexidine (for pharmaceutical use), polyhexamethylene biguanidine hydrochloride (for food factory), etc., and the agent exhibits antibacterial power against Gram-positive bacteria and Gram-negative bacteria at a low concentration. The content of the biguanide compound in the agent is 2-50%. Sufficient effect can be attained by adding the fungicide to the biguanide-based antibacterial agent in an amount of 50-5,000ppm for a halogen-containing nitrogen compound and 10-1,000ppm for an isothiazoline compound. There is no contamination of the antibacterial agent with fungi and the secondary contamination of a food factory, etc., caused by the contamination of the antibacterial agent can be prevented by the use of the antibacterial agent.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fungicide containing a biguanide fungicide and a fungicide which eliminates secondary contamination by fungi.

[0002]

BACKGROUND OF THE INVENTION Biguanide fungicides are widely used in food factories because they have low toxicity and show strong antibacterial activity against Gram-positive / negative bacteria.

[0003]

Generally, since the bactericide also has a fungicidal effect, the bactericidal component has a high concentration (for example, 1%).
It is not common knowledge that the fungicide present in (1) above is contaminated with mold, and therefore it has been conventionally considered that it is not necessary to add a fungicide to the fungicide at all. However, the present inventors have found for the first time that with regard to the biguanide fungicides, the fungicides themselves are contaminated by certain molds, and therefore their use may cause secondary contamination of food factories. .

[0004]

[Means for Solving the Problems] Therefore, as a result of various investigations by the present inventors, by adding and mixing a fungicide to a biguanide fungicide, there is no mold in the fungicide,
It has been found that it is possible to obtain a fungicide without secondary contamination of mold in a food factory.

That is, the present invention relates to a biguanide fungicide containing a fungicide. Conventionally, it is known that a biguanide fungicide also has a fungicidal action, and it is not common sense that the fungicide itself is contaminated by mold. Therefore, it is necessary to add a fungicide to the biguanide fungicide. The need is entirely unexpected.

As the biguanide fungicides in the present invention, there are chlorhexidine, polyhexamethylene biguanidine hydrochloride and the like. Since chlorhexidine has low toxicity, it is widely used for medical purposes, and polyhexamethylene biguanidine hydrochloride is a food factory. It is a known substance used for sterilization of machinery, equipment, containers, and factory environment. Biguanide fungicides show strong bactericidal activity against gram-positive and gram-negative bacteria at low concentrations. According to experiments conducted by the inventor, the MIC of polyhexamethylene biguanidine hydrochloride for gram-positive bacteria and gram-negative bacteria was several tens to several hundreds ppm, and the MIC for mold was several hundreds to several thousand ppm. The biguanide fungicide of the present invention can contain other fungicides, surfactants, food dyes, other components such as solvents such as water, and the fungicides chlorhexidine and polyhexamethylene biguanidine hydrochloride. The content of biguanide compound such as is preferably 1 to 100%, and usually 2 to 50% in the fungicide.

The fungicide in the present invention may be a known substance and is not particularly limited, but one having high safety is desirable.
Examples include alcohols such as ethanol and isopropyl alcohol, organic acids such as sorbic acid, dehydroacetic acid, paraoxybenzoic acid ester, thiabendazole, halogen-containing nitrogen-sulfur compounds, isothiazoline-based compounds, etc. , Isothiazoline compounds are preferred.

In the present invention, the addition amount of the fungicide to the biguanide fungicide is not related to the concentration of the active ingredient (bactericidal component) of the biguanide fungicide, and the fungicide in the fungicide has the fungicidal effective concentration thereof. And added so that they are sufficiently homogeneous. For example, the halogen-containing nitrogen-sulfur compound in the bactericide may be 50 ppm or more, preferably 5 ppm.
0-5000ppm is good, isothiazoline compounds are 10ppm or more in the bactericide, preferably 10-1000p
pm is good. The bactericide of the present invention may be used as it is,
It may be appropriately diluted with water before use.

[0009]

Examples of the present invention will be described below. Example 1 To a glass beaker, 1 kg of a 20% aqueous solution of polyhexamethylene biguanidine hydrochloride was added, and 500 mg of a halogen-containing nitrogen-sulfur compound (trade name; Slaoff 620 (manufactured by Takeda Pharmaceutical Co., Ltd.)) was added, and the mixture was stirred for 30 minutes with a stirrer. The mixture was stirred and mixed to obtain the bactericide X of the present invention.

EXAMPLE 2 500 g of a 20% aqueous solution of polyhexamethylene biguanidine hydrochloride and 50 g of benzalkonium chloride were placed in a glass beaker.
% Aqueous solution 100 g, polyoxyethylene alkyl ether 100 g, and isothiazoline compound (trade name; San-Ivac IT-20BT (manufactured by San-ai Petroleum Co., Ltd.))
Was added to 200 ml of water, and the mixture was stirred and mixed with a stirrer for 30 minutes to obtain a bactericide Y of the present invention.

Example 3 The following experiment confirmed that the fungicides of Examples 1 and 2 were free of mold. The fungicidal prevention effect of the fungicides of Examples 1 and 2 was examined using molds of the genus Pesidomyces, which is a common mold causing contamination. After the test products shown in Table 1 were sterilized by filtration, the suspension of Pescidomyces (10 ⁵ /
0.5 ml) to 50 ml of the test product, and add 1 at 25 ° C.
Allow to stand overnight, filter through a membrane filter, and put the membrane filter on potato dextrose agar for 25
After culturing at 7 ° C for 7 days, the effect was judged by the presence or absence of mold growth.
The results are shown in Table 1. Mold growth was not recognized in the bactericide of the present invention, and it was confirmed that mold was not mixed.

[0012]

[Table 1] Table 1 Test product Presence or absence of mold fungicide X (Example 1) No mold occurrence Fungus disinfectant X excluding fungicides Large amount of mold fungicide Y (Example 2) No mold occurrence Sterilization Agent Y excluding fungicides A large amount of mold is generated

[0013]

The fungicide of the present invention does not contaminate the fungicide itself with the fungicide of the present invention.
In food factories, etc., it is possible to prevent secondary contamination of mold due to contamination of the fungicide itself.

Claims (2)

[Claims] 1. A biguanide fungicide containing a fungicide. 2. The fungicide according to claim 1, which contains a nitrogen halide sulfur compound or an isothiazoline compound as a fungicide.