JP2008255078A - Humectant, anti-aging agent, skin beautifying agent, antioxidant, slimming agent, treatment agent, arginase activity accelerator and skin external preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide humectants, anti-aging agents, skin beautifying agents, antioxidants, slimming agents, treatment agents, and arginase activity accelerators, which are widely applicable to the fields such of skin external agents. <P>SOLUTION: The humectants, anti-aging agents, skin beautifying agents, antioxidants, slimming agents, treatment agents, arginase activity accelerators, and skin external agents each comprise, as an effective ingredient, an extract of a plant belonging to genus Cynanchum, Asclepiadaceae family, for example, Cynanchum atratum, Cynanchum caudatum, Cynanchum versico, Cynanchum paniculatum, or Cynanchum wilfordii. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

The present invention relates to a moisturizing agent, an anti-aging agent, a whitening agent, an antioxidant, a slimming agent, a treatment agent, an arginase activity promoter and an external preparation for skin, which comprise a naturally derived ingredient as an active ingredient. More specifically, a moisturizer, an anti-aging agent, a whitening agent, an antioxidant, a slimming agent, a treatment agent, an arginase activity promoter and an external preparation for skin containing the plant of the genus Asclepiadaceae or Cynanchuum. About.

  Causes of skin symptoms such as aging, disease, stress, UV wrinkles, stains, reduced skin elasticity, dryness, decreased cellular function, melanin production and pigmentation due to UV rays, decrease and degeneration of dermal matrix components, UV rays, etc. Oxidative damage of cells due to. In order to prevent and ameliorate such skin symptoms, various active ingredients have been searched and formulated. In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and so far, applications of extracts such as plants and fungi to skin external preparations have been studied.

  For example, as a moisturizing agent having excellent moisturizing effect and safety, an extract of the genus Asteraceae (see Patent Literature 1) and an extract of the Brassicaceae Repidium plant (see Patent Literature 2) are known. In order to obtain an external preparation for skin having anti-aging and improving effects on skin, essence of Ponkan (see Patent Document 3) as an ingredient having an activity of promoting dermal fibroblasts or promoting proliferation (see Patent Document 3) (Patent Document) 4), chlorella extract (see Patent Document 5), and loquat extract (see Patent Document 6). As a whitening agent, an extract of white crane ganoderma (see Patent Document 7), as an antioxidant, an extract of a plant belonging to the genus Salogaceae (see Patent Document 8), a mammal as a component that suppresses fat accumulation in the living body. Milk-derived phospholipids (see Patent Document 9), brown algal enzyme degradation product (see Patent Document 10), Arginase activity promoter as a tree extract (see Patent Document 11), bellflower extract (see Patent Document 12), Mumon winter extract (see Patent Document 13) and apricot fruit (see Patent Document 14) are known.

JP 2002-20262 A JP 2005-281271 A JP 2001-131045 A JP 2000-178198 A JP 11-335293 A Japanese Patent Publication No. 5-17206 JP 2003-89630 A Japanese Patent Laid-Open No. 10-182413 JP 2001-275614 A JP-A-7-278005 Japanese Patent Laid-Open No. 10-7581 JP 2002-29983 A JP 2003-277285 A JP 2006-143608 A

  Thus, various naturally-derived components have been applied so far. However, there are many naturally-derived ingredients whose effects are not yet known. Excellent moisturizing action, anti-aging action, whitening action, antioxidant action, slimming action, treatment action, arginase activity promoting action Development of an active ingredient having such as was expected.

The present inventors have made examined various components of natural origin, conventionally the effect was not known Asclepiadaceae (Asclepiadaceae) vincetoxicum (Cynanchum) moisturizing effect having excellent plant or an extract thereof, The present inventors have found that an anti-aging action, a whitening action, an antioxidant action, a slimming action, a treatment action, and an arginase activity promoting action exist, and have further studied and completed the present invention.

That is, the present invention is a humectant to Asclepiadaceae (Asclepiadaceae) vincetoxicum (Cynanchum) 1 or more kinds of plants or active ingredient the extract is selected from plant, anti-aging agents, whitening agents, anti-oxidants , Slimming agents, treatment agents or arginase activity promoters.

Another invention relates to a skin external preparation characterized by containing one or more plants selected from Asclepiadaceae plants and cynanchuum plants or extracts thereof.

According to the present invention, a moisturizer and an anti-aging agent having an excellent effect by using as an active ingredient one or two or more kinds of plants selected from the genus Asclepiadaceae, Cynanchum, or an extract thereof. Agents, whitening agents, antioxidants, slimming agents, treatment agents and arginase activity promoters can be provided.

  By adding these moisturizers, anti-aging agents, whitening agents, antioxidants, slimming agents, treatment agents, and arginase activity promoters to skin external preparations such as cosmetics and topical medicines, wrinkles, tarmi and skin elasticity It is possible to provide various compositions that exhibit an excellent effect in preventing and improving various skin symptoms such as reduction, spots, and dullness.

Asclepiadaceae (Asclepiadaceae) vincetoxicum The (Cynanchum) plants, Funabarasou (Cynanchum atratum Bunge; Vincetoxicum hirundiaria) , Ikema (.. Cynanchum caudatum (Miq) Maxim), Byakubi (Cynanchum versico Bunge), Suzusaiko (Cynanchum paniculatum (Bunge) Kitagawa), Koikema (Cynanchum wilfordii (Maxim.) Hemsl .) is known.

  These plants can be used alone or in combination of two or more.

The present invention is not particularly limited as long as it is a member of the genus Camellia belonging to the genus Turtle, but for reasons such as being relatively easy to obtain, a suitable example is Ikenma ( Cynanchum caudatum (Miq.) Maxim.).

  There are no particular restrictions on the site of use when using these genus Camellia plants, and any part such as roots, stems, trunks, leaves, and flowers can be used. A plurality of parts may be used in combination.

  They can be pulverized as they are, but it is preferable to use an extract from those parts.

  For extraction, any part of the genus Camellia species may be used, but whole plants, roots, leaves, flowers, etc. may be used for convenient use. In that case, you may make it obtain an extract using a some site | part. Further, two or more kinds of extracts extracted using different solvents may be mixed and used.

  In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization.

  The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, stirring or homogenization in an extraction solvent may be performed. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

  As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether Solvents such as esters; esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline, and the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical liquids and subcritical liquids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The above-mentioned extract of the genus Camellia belonging to the genus Camellia can be used as it is, but it can be left to mature for a certain period of time, or the concentrated and dried product can be used again in water or a polar solvent. It can also be used by dissolving. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, desalting, etc., or fractionation treatment by column chromatography or the like, as long as these physiological functions are not impaired. The above-mentioned extract of the genus Camellia spp., The processed product and the fraction thereof can be freeze-dried after each treatment and fractionation, and dissolved in a solvent at the time of use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  The genus Camellia spp. Or its extract has excellent moisturizing, anti-aging, whitening, antioxidant, slimming, treatment, arginase activity promoting, moisturizing, anti-aging, whitening. It can be used as an antioxidant, slimming agent, treatment agent, and arginase activity promoter.

  Each of these agents is not limited at all in terms of its form and presence / absence of other components as long as it contains the genus Camellia plant or its extract as an active ingredient. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use and the like, vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

  A moisturizing agent containing an active ingredient of the genus Camellia spp. Or an extract thereof can give an excellent moisturizing effect to the skin and hair.

  An anti-aging agent comprising an active ingredient of the genus Camellia spp. Or its extract has an excellent cell activating effect on human dermal fibroblasts and an effect of promoting collagen production, and has an excellent effect on the prevention and improvement of aging symptoms. Demonstrate.

  A whitening agent comprising a genus Gagamella plant or an extract thereof as an active ingredient has an excellent inhibitory effect on tyrosinase activity of human epidermal melanocytes, and prevents and improves pigmentation, blemishes, freckles, etc. Demonstrate the effect.

  Antioxidants containing the genus Camellia plant or its extract as an active ingredient have an excellent radical scavenging effect and a superoxide anion scavenging effect, and exhibit an excellent antioxidant action.

  A slimming agent comprising a genus Camellia plant or an extract thereof as an active ingredient has an excellent effect of suppressing neutral fat accumulation in human preadipocytes and exhibits an excellent slimming action.

  A treatment agent containing the genus Camellia genus plant or its extract as an active ingredient is effective in improving skin symptoms such as rough skin, fine lines and dullness, and can improve skin texture and enhance skin transparency. it can.

  An arginase activity promoter comprising a genus Camellia plant or an extract thereof as an active ingredient has an excellent arginase activity promoting effect on human keratinocytes, and exhibits an excellent arginase activity promoting action.

  Prevention of various skin symptoms such as wrinkles, tarmi, blemishes, dullness, dryness and fine wrinkles by blending the genus Camellia genus plant or its extract with topical skin preparations such as cosmetics, topical medicines and quasi drugs -It is possible to obtain an external preparation for skin that exhibits an excellent effect of improvement. Therefore, for example, it can be preferably used as a skin external preparation for moisturizing, a skin external preparation for preventing aging, a skin external preparation for slimming, or a skin external preparation for whitening.

  The amount of the genus Camellia spp. Or its extract in the external preparation for skin can be adjusted according to the type and purpose of the external preparation for skin, but from the standpoint of efficacy and stability, the total amount of external preparation for skin In terms of solid content, 0.0001 to 10.0 mass% is preferable, more preferably 0.001 to 5.0 mass%, still more preferably 0.01 to 5 mass%, and still more preferably. Is 0.1-5 mass%.

  The dosage form of the external preparation for skin is arbitrary. For example, it should be provided in various dosage forms such as lotion, emulsion, gel, cream, ointment, powder, granule, pack, patch, patch, aerosol, etc. Can do.

  In addition to the genus Camellia gull plant or its extract, the topical skin preparation is usually formulated into pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics, and cleansing agents, etc. as necessary and necessary. Optional ingredients such as water, oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, gelling agents, detergents, UV absorbers, anti-inflammatory agents, thickeners, pH adjusters, chelating agents Drugs (medicinal ingredients), fragrances, resins, preservatives, alcohols and the like can be appropriately blended. In addition, other moisturizers, anti-aging agents, whitening agents, antioxidants, slimming agents, treatment agents, arginase activity promoters or plants other than the genus Camellia sp. It can also be used in combination with goods.

Hereinafter, preparation examples of the plant extract of the genus Asclepiadaceae ( Cynanchuum ), tests for evaluating each action, formulation examples as external preparations for skin, and use tests will be described in more detail. The target range is not limited at all by this.

[Preparation Method 1]
20 kg of 50% ethanol aqueous solution was added to 1 kg of dry ground pulverized leaves of Ikema ( Cynanchum caudatum (Miq.) Maxim.) And immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, 30 g of a genus Camellia plant extract was obtained.

[Preparation Method 2]
20 kg of water was added to 1 kg of dried crushed leaves of Ikema and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration and the solvent was removed, to obtain 20 g of the genus Camellia plant extract.

[Preparation Method 3]
20 kg of methanol was added to 1 kg of dried crushed whole of Ikema and immersed for 7 days at room temperature. The extract was collected by filtration and the solvent was removed, and then 50 g of the genus Camellia plant extract was obtained.

[Preparation Method 4]
100 g of dried crushed whole Ikema plant was put into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under an atmospheric pressure of 15 Mpa. The extract was recovered to obtain 10 g of the extract of the plant belonging to the genus Gullaceae.

<Evaluation 1 of anti-aging action (cell activation action)>
Evaluation of the cell activation effect of the genus Camellia plant extract was performed by the method shown below. As the sample, the extract of the genus Camellia belonging to the genus Camellia belonging to the preparation method 2 was used.

Normal human dermal fibroblasts manufactured by Kurabo Industries Co., Ltd. (Kurashiki Boseki Co., Ltd.) were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 1% by mass of fetal bovine serum (FBS) was added was used. After culturing for 24 hours, the medium was replaced with 1% by mass FBS-added DMEM medium to which samples (extracts) were added so that the concentrations shown in Table 2 were obtained, and further cultured for 48 hours. After removing the supernatant, the medium was replaced with a medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for about 2 hours. Thereafter, formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. In the evaluation, in addition to the medium containing the sample, 1% by mass FBS-added DMEM medium was used as a control.

  The evaluation results are shown in Table 1 as relative values when the cell activation effect in the control is 100.

As is clear from Table 1, a significant cell activation effect was observed in the medium to which the extract of the genus Camellia was added. From this, it was clarified that the extract of the genus Camellia belonging to the genus Camellia has an excellent cell activation effect.
<Evaluation 2 of anti-aging action (collagen production promoting action)>
Evaluation of the collagen production promoting action of the genus Camellia plant extract was performed by the following method. As the sample, the extract of the genus Camellia belonging to the genus Camellia belonging to the preparation method 2 was used.

Normal human dermal fibroblasts manufactured by Kurabo Industries Co., Ltd. (Kurashiki Boseki Co., Ltd.) were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS). After culturing for 24 hours, the medium was replaced with 0.5% by mass FBS-added DMEM medium added with a sample (extract) so as to have each concentration shown in Table 7, and further cultured for 24 hours. The ELISA method was used for the quantification of type I collagen secreted into the culture supernatant, and finally 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) against labeled peroxidase. And hydrogen peroxide were added and reacted, and the absorbance at 405 nm was measured with a microplate reader. The amount of protein was measured with BCA Protein Assay Kit manufactured by PIERCE, and the amount of type I collagen produced per unit protein was determined.

  The evaluation results are shown in Table 2 as relative values when the amount of type I collagen produced per unit protein in the control without addition of the sample is 100.

  As is clear from Table 2, a significant collagen production promoting effect was observed in the medium to which the extract of the genus Camellia was added. From this, it was clarified that the extract of the genus Camellia belonging to the genus Camellia has an excellent collagen production promoting action.

  From the above, it has been clarified that the extract of the genus Camellia spp. Has an excellent anti-aging effect.

<Evaluation of whitening action (tyrosinase activity inhibitory action)>
Evaluation of the tyrosinase activity inhibitory action of the extract of the genus Camellia spp. Was performed by the following method. As the sample, the extract of the genus Camellia sp.

Normal human epidermal melanocytes manufactured by Kurabo Industries Co., Ltd. were seeded in a 96-well microplate so as to be 3.0 × 10 ⁴ cells per well. As a seeding medium, Medium154S manufactured by Kurabo Industries Co., Ltd. was used. After 24 hours, the medium was replaced with a sample-added medium adjusted to each concentration shown in Table 3 with Medium 154S, followed by further culturing for 48 hours. Next, the cells were completely lysed by exchanging with 75 μL of 1% by weight Triton-X-containing phosphate buffer, and 50 μL of the cells was used as a crude enzyme solution. To the crude enzyme solution, 50 μL of 0.05% by mass L-dopa-containing phosphate buffer serving as a substrate was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm was measured immediately after the addition of the substrate and at the end of the reaction with a microplate reader, and the amount of produced dopamelanin was determined by introducing each measured value into equation (1).

Formula (1): Amount of produced dopamelanin = {(405 nm value after reaction−405 nm value before reaction)} − 2.166 / 5.238
Moreover, the protein amount of each well was measured by BCA Protein Assay Kit made by PIERCE, and the amount of dopamelanin produced per unit protein amount was determined.

  The measurement results are shown in Table 3 together with the values of the controls using the medium without the sample.

  As is clear from Table 3, when a medium supplemented with the extract of the genus Camellia was used, a decrease in melanin production was observed. From this, it was found that the extract of the genus Camellia belonging to the genus Gagaide has an excellent tyrosinase activity inhibitory action.

  From the above, it has been clarified that the extract of the genus Camellia belonging to the genus Camellia has an excellent whitening effect.

<Evaluation of antioxidative action (radical scavenging action) 1>
Evaluation of the antioxidant activity of the extract of the genus Camellia spp. Was performed by the following method. As the sample, the extract of the genus Camellia belonging to the genus Camellia belonging to the preparation method 2 was used.

  The concentration of each sample was adjusted using 50% by mass ethanol to obtain a sample solution, and 100 μL each was added to a 96-well microplate so that the concentrations shown in Table 5 were obtained. Thereto was added 100 μL of 0.2 mM 1,1-diphenyl-2-picrylhydride radical (DPPH) ethanol solution, mixed well, and allowed to stand at room temperature in the dark for 24 hours. Thereafter, the absorbance at 516 nm derived from the DPPH radical was measured. When the absorbance of the control when the sample was not added was (A) and the absorbance when the sample was added was (B), the elimination rate of the DPPH radical was introduced into Equation (2) and obtained. Table 4 shows the measurement results.

      Formula (2): Radical scavenging rate = {1- (B) / (A)} × 100 (%)

  As is clear from Table 4, an excellent DPPH radical scavenging effect was recognized in the genus Camellia plant extract. From this, it was found that the extract of the genus Camellia spp. Has an excellent radical scavenging action.

<Evaluation 2 of Antioxidant Action (Superoxide Anion Scavenging Action)>
Evaluation of the superoxide anion scavenging action of the genus Camellia plant extract was performed by the following method. As the sample, the extract of the genus Camellia belonging to the genus Camellia belonging to the preparation method 2 was used.

  To 75 μL of Hanks (+) solution containing 0.25 mM WST-1 and 1 mM Hypoxanthine, 25 μL of a sample diluted with Hanks (+) solution to a concentration shown in Table 6 was added, and 25 μL of Xanthine Oxidase (0.0075 Units) was added. In addition, after reacting at 37 ° C. for 15 minutes, absorbance at 450 nm was measured. When the absorbance of the control with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (3) was defined as the superoxide anion elimination rate. The evaluation results are shown in Table 5.

      Formula (3): Erase rate = {1- (B) / (A)} × 100 (%)

  As is clear from Table 5, the superoxide anion scavenging effect was recognized excellent in the genus Camellia plant extract. From this, it was found that the extract of the genus Camellia belonging to the genus Camellia has an excellent superoxide anion scavenging action.

  From the above, it has been clarified that the extract of the genus Camellia spp.

<Evaluation of slimming action (inhibition of neutral fat accumulation)>
Evaluation of the neutral fat accumulation inhibitory action in the adipocytes of the genus Camellia plant extract was performed by the following method. As the sample, the extract of the genus Camellia sp.

Subcutaneous fat-derived normal human preadipocytes Cryo • HPRAD-SQ (Sanko Junyaku Co., Ltd.) were seeded on a 96-well microplate so that the number was 1.0 × 10 ⁴ per well. PGM medium (containing 10% by mass fetal bovine serum (FBS), 2 mM L-Glutamine, 100 units / mL Penicilline, 100 μg / mL Streptomycine) was used as the seeding medium. Immediately before the cells are saturated, the medium is changed to PGM differentiation medium (containing 10 μg / mL insulin, 1 μM Dexamethasone, 200 μM Indomethacin, 500 μM Isobutyl-methylxanthine) to which the samples shown in Table 4 are added, and induction of differentiation into adipocytes Went. After initiation of differentiation induction, the cells were cultured for 10 to 14 days until the control group matured and many lipid droplets accumulated in the cells. After the cells were collected, the cells were fixed using a 10% neutral buffered formaldehyde solution. After washing with PBS, 0.5 w / v% Oil Red O solution was added and incubated at 37 ° C. for 2 hours. After washing with PBS, methanol was added to extract the dye. After extraction, the absorbance at 550 nm was measured from a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the amount of accumulated triglyceride was measured using the difference between the two measured values.

  The measurement results are shown in Table 6 in terms of relative values with the neutral fat accumulation amount being 100 in the control using the medium without the sample.

  As is clear from Table 6, a significant neutral fat accumulation inhibitory effect was observed in the medium to which the extract of the genus Camellia was added. From this, it was clarified that the extract of the genus Camellia spp.

  From the above, it was clarified that the extract of the genus Camellia belonging to the genus Camellia has an excellent slimming action.

<Evaluation of arginase activity promoting action>
Evaluation of the arginase activity promoting action of the genus Camellia plant extract was carried out by the following method. As the sample, the extract of the genus Camellia sp.

Human epidermal keratinocytes HaCaT were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with a 5% by mass FBS-added differentiation-inducing medium containing 1.2 mM CaCl ₂ added with a sample (extract) so as to have each concentration shown in Table 1, and further cultured for 9 days. The medium was exchanged once every 3 days. Urea nitrogen B-Test Wako (Wako Pure Chemical Industries) was used for quantification of urea secreted into the culture supernatant. Arginase hydrolyzes arginine to produce ornithine and urea. Urea is decomposed into ammonia by urease, and ammonia reacts with salicylic acid and hypochlorous acid in the presence of sodium pentacyanonitrolyl iron (III) dihydrate to form indophenol. Under alkaline conditions, the absorbance at 570 nm derived from indophenol was measured with a microplate reader, the urea concentration was determined, and the arginase activity was quantified. The amount of protein was measured with BCA Protein Assay Kit manufactured by PIERCE, and the arginase activity per unit protein amount was determined.

  The evaluation results are shown in Table 7 as relative values when the arginase activity per unit protein amount in the control with no sample added is defined as 100.

  As is clear from Table 7, a significant arginase activity promoting effect was observed in the medium to which the extract of the genus Camellia was added. From this, it was clarified that the extract of the genus Camellia belonging to the genus Gagaide has an excellent arginase activity promoting action.

  Then, the formulation example of the external preparation for skin which mix | blended the genus Camellia genus plant extract obtained by said each preparation method is shown.

[Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 100 (11) Arginine (1% by weight aqueous solution) 20.0
(12) Sea urchin family gull genus plant extract [Preparation method 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification, cooling is started, and (11) and (12) are sequentially added and mixed uniformly.

[Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 100 (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) An extract of the genus Camellia spp. [Preparation method 3] 1.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Remainder 100 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Sea urchin family gull genus plant extract [Preparation method 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added after completion | finish of emulsification, cooling is started, (12) is added at 40 degreeC, and it mixes uniformly.

[Example 4] Cosmetic liquid (1) The balance (mass%) of purified water 100
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) An extract of the genus Camellia spp. [Preparation Method 2] 3.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C., add (16) and mix evenly.

[Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) The remainder (3) made into purified water 100 (3) Sodium hydroxide (10 mass% aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract from the genus Camellia family [Preparation method 2] 0.5
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Example 6] Cleansing fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Remainder as purified water 100 (4) Extract from the genus Gullaceae [Preparation method 3] 4.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 25.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) The remainder (8) made from purified water 100 (Extracted from the genus Camellia) [Preparation method 3] 0.1
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Example 8] Make-up base cream (1) Squalane 10.2 (% by mass)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) The balance (100) of purified water 100 (titanium oxide) 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract from the genus Gullaceae [Preparation Method 2] 3.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, dissolved by heating at 75 ° C., the pigments (8) to (10) are added thereto, and the mixture is uniformly dispersed with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Remainder water 100 (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Sea urchin family gull genus plant extract [Preparation method 1] 0.5
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and the components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Example 10] Water-in-oil emollient cream (1) Liquid paraffin 34.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) An extract of the genus Camellia spp. [Preparation Method 1] 3.0
(11) The balance of 100 purified water (12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melted at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. with stirring, and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Example 11] Pack (1) The balance (mass%) of purified water 100
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 9.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Species of the genus Camellia family [Preparation method 2] 1.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Example 12] Bath agent (1) Fragrance 0.3 (mass%)
(2) Extract from the genus Gullaceae [Preparation method 3] 3.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 46.7
Production method: (1) to (4) are mixed uniformly.

[Example 13] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water, the remainder (100) (11) Species of the genus Gullaceae [Preparation method 3] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Example 14] Hair artic (1) Ethanol 50.0 (mass%)
(2) Purified water, the remainder (100) (3) Species of the genus Gullaceae [Preparation method 1] 3.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

<Evaluation of moisturizing action>
A use test using the external preparation for skin of Examples 1 and 3 was performed, and the moisture retention of the genus Gullaceae plant extract was evaluated. At that time, the extract of the genus Camellia belonging to the genus Camellia belonging to Example 1 was replaced with 50% by mass ethanol aqueous solution, and the extract of the genus Camellia belonging to the genus Camellia belonging to Example 1 was compared with 50% by mass A usage test was conducted in the same manner as Comparative Example 2 except that the ethanol aqueous solution was used.

  A group of 15 male and female panelists were allowed to use each sample blindly for one month, and evaluated in three stages: “feel”, “cannot say”, and “do not feel”. Table 8 shows the number of panelists that obtained each evaluation.

  From Table 8, it was found that 80% or more of panelists could feel clear moisture retention in the use group of Examples blended with the extract of the genus Camellia. From this, it was clarified that the extract of the genus Camellia spp. Has an excellent moisturizing action.

<Evaluation of treatment action>
The use test using the skin external preparations of Examples 1 and 3 and Comparative Examples 1 and 2 was conducted, and the improvement effect of those symptoms was evaluated with respect to rough skin, skin texture, and skin transparency.

  For each sample, a group of 20 male and female panelists in their 20s to 50s who are worried about rough skin, skin texture, and skin transparency are used for 1 month each blind, and changes in skin condition before and after use. Observed and evaluated. As indicators of skin condition, rough skin, skin texture, and skin transparency were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. The rough skin and the transparency of the skin were visually evaluated, and the texture of the skin was observed using a microscope. Table 9 shows the number of panelists that obtained each evaluation.

  From Table 9, in the use groups of Comparative Examples 1 and 2 that do not contain the extract of the genus Camellia belonging to the genus Camellia family, no improvement was observed in more than half of the panelists regarding rough skin, skin texture, and skin transparency. In the example use group in which the plant gull genus plant extract was blended, a clear improvement was observed in 70% or more of the panelists. From this, it was found that the extract of the genus Camellia belonging to the genus Camellia family has an excellent effect in improving skin symptoms in rough skin, skin texture, and skin transparency.

  From the above, it has been clarified that the extract of the genus Camellia spp. Has an excellent treatment action.

Claims (8)

Asclepiadaceae (Asclepiadaceae) vincetoxicum (Cynanchum) 1 or more kinds of plants or humectant to the extract as an active ingredient selected from plants. An anti-aging agent comprising, as an active ingredient, one or more plants selected from Asclepiadaceae plants and cynanchuum plants or extracts thereof. Asclepiadaceae (Asclepiadaceae) vincetoxicum (Cynanchum) whitening to one or more plants or active ingredient the extract is selected from plant. An antioxidant comprising one or more plants selected from Asclepiadaceae and Cynanchum plants or extracts thereof as an active ingredient. Asclepiadaceae (Asclepiadaceae) vincetoxicum (Cynanchum) 1 or more kinds of plants or slimming agent to the extract as an active ingredient selected from plants. Asclepiadaceae (Asclepiadaceae) vincetoxicum (Cynanchum) treatment agent to one or more plants or active ingredient the extract is selected from plant. An arginase activity promoter comprising, as an active ingredient, one or more plants selected from Asclepiadaceae plants and cynanchuum plants or extracts thereof. A skin external preparation characterized by containing one or more plants selected from Asclepiadaceae plants and cynanchuum plants or extracts thereof.