JP2001046030A - Production of blueberry-extracted essence - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an essence containing a high anthocyanidin glucoside content by using blueberry as a raw material. SOLUTION: This method for producing a blueberry-extracted essence is provided by using blueberry as a raw material, extracting it by using any of an aqueous acidic solution, alcohol containing an acid or alcohol containing water, adsorbing by passing the extract through a synthetic adsorbent, desorbing an anthocyanidin glucoside from the synthetic adsorbent, concentrating and drying.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a method for producing a blueberry extract. In particular, the present invention relates to blueberries (Vacciniummyrtill).
usL. ), An extract containing an anthocyanidin glycoside is efficiently extracted, purified, and dried.

[0002]

2. Description of the Related Art Extracts of blueberries and fruits have long been used in Europe and the United States as pharmaceuticals for cardiovascular diseases such as vascular disorders and diabetes, or as pharmaceuticals and eye health foods in the field of ophthalmology. Also in Japan, there are expectations for use as functional foods for the treatment and prevention of heart disease, diabetes and other lifestyle-related diseases and for the prevention of eye strain. As for the physiological function of the blueberry extract, various studies have shown that it has the following physiological effects.

[0003] The effect of promoting regeneration of rhodopsin on the retina and the effect of dark adaptation. Capillary protection effect. Antitumor effect. Improving effect of circulatory system disorders such as hypertension and arteriosclerosis. Antiinflammatory effect. Radical scavenging effect. The active ingredient of the blueberry extract is considered to be a glycoside using anthocyanidins as aglycones, and there are five types of aglycones and three types of sugars to be bound, and there are 15 types of anthocyanidin glycosides depending on the combination of each type. Is known (Chemical 1, Table 1).

[0004]

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[0005]

[Table 1]

[0006] The method for producing such blueberry extract includes the ericaceae blueberry (Vaccini).
u-dish myrtillus L. ) Is known to be obtained by extraction with juice or water. However, the extraction efficiency was poor, it was difficult to extract on an industrial scale, and the content of anthocyanidin glycoside was low.

[0007]

An object of the present invention is to produce an extract having a high content of anthocyanidin glycosides from blueberries.

[0008]

Means for Solving the Problems The present inventor has made intensive efforts to solve the above problems, and as a result, using blueberry as a raw material, extraction is performed using an acidic aqueous solution, an alcohol containing an acid or a hydroalcohol, It has been found that the problem can be solved by allowing the extract to be adsorbed by passing through a synthetic adsorbent, and concentrating and drying the liquid obtained by desorbing the anthocyanidin glycoside from the synthetic adsorbent.

That is, the present invention provides: (1) Extraction using blueberry as a raw material, using either an acidic aqueous solution, an acid-containing alcohol or an aqueous alcohol, (2) solid-liquid separation of the extract, (3) solid-liquid extract 1. a method for producing a blueberry extract, the method comprising: adsorbing an anthocyanidin glycoside on a synthetic adsorbent to desorb anthocyanidin glycoside from the synthetic adsorbent; and (4) concentrating and drying the desorbed solution. The pH of any one of an acidic aqueous solution, an alcohol containing an acid, and a hydrous alcohol is 2 or less.
The present invention relates to a method for producing the described blueberry extract.

Specific examples of the synthetic adsorbent in the present invention include "Diaion HP resin", "Diaion SP resin" (manufactured by Mitsubishi Chemical Corporation), and "Amberlite XAD resin".
(Made by Rohm and Haas), "Duolite S resin"
(Manufactured by Diamond Shamrock Co., Ltd.).

(Raw Materials) In the method for producing a blueberry extract according to the present invention, an extract containing an anthocyanidin glycoside is used as a raw material. Vac, a particularly preferred raw material
ciniummyrtillus L .; Is Bilberry (B
ilberry), a wild European species and abundant in Northern Europe. This wild species has a high anthocyanidin glycoside content and is therefore an optimal raw material when producing a blueberry extract.

(Extraction) In the method for producing a blueberry extract according to the present invention, a raw material containing an anthocyanidin glycoside is subjected to extraction using an acidic aqueous solution, an alcohol containing an acid, or a hydrous alcohol. By performing extraction using an acidic aqueous solution, an alcohol containing an acid, or a hydrous alcohol, an anthocyanidin glycoside can be efficiently extracted. Acids used in the extraction using an acidic aqueous solution include hydrochloric acid and sulfuric acid. In particular, the pH of the acidic aqueous solution is important for efficiently extracting anthocyanidin glycosides, and the pH is preferably 2 or less.

According to the present invention, the extraction can be carried out at a temperature from room temperature to near the boiling point. However, in consideration of the thermal stability of the anthocyanidin glycoside, it is preferably from room temperature to 40 ° C.
° C. The extraction time can be set as appropriate, but it is preferable to extract overnight, judging from industrial operability. As the extraction method, mixing stirring extraction, circulation extraction can be used,
In consideration of operability, mixing and stirring extraction is preferable. The number of extractions is preferably repeated several times to increase the yield.

(Solid-Liquid Separation) The extract obtained using an acidic aqueous solution, an alcohol containing an acid, or a hydroalcohol contains pericarp and seeds in blueberries and is removed. For solid-liquid separation, an industrially used solid-liquid separator can be used, and a general pressure filter can also be used.

(Purification) The extract obtained by solid-liquid separation contains anthocyanidin glycosides. In order to increase the content of anthocyanidin glycosides, the extract is passed through a synthetic adsorbent, and the anthocyanidin glycosides are passed through. After the impurities are washed with water, the anthocyanidin glycoside content is increased by desorbing the anthocyanidin glycoside from the synthetic adsorbent using an organic solvent or a mixed solvent of an organic solvent and water.

(Dry) A blueberry extract can be prepared by concentrating and drying the desorbed liquid from the synthetic adsorbent. Concentration can be performed by general vacuum concentration or heat concentration. However, considering the stability of the anthocyanidin glycoside, vacuum concentration is preferable. Drying includes general vacuum drying, spray drying, freeze drying and the like, and can be selected according to the intended use of the blueberry extract. The blueberry extract obtained by the production method according to the present invention can be used as a health food in various forms depending on the intended use, but can be used particularly as a soft drink or as a tablet.

[0017]

[Example 1] 200 g of frozen blueberry fruit (total anthocyanidin content 0.39
%), Adjust the pH to 1 with hydrochloric acid, and extract at room temperature with stirring overnight. The next morning, the mixture is filtered and passed through a column filled with 100 mL of Diaion HP20 resin. After passing the solution, it is washed with 300 mL of water, and then adsorbed anthocyanidin glycoside is desorbed by flowing 400 mL of 80% ethanol. The desorbed solution was concentrated under reduced pressure and freeze-dried to obtain 1.88 g of a blueberry extract (total anthocyanidin content of 36.85).
%).

Example 2 To 200 g of frozen blueberry fruit (total anthocyanidin content 0.39%), 1000 mL of water was added, and the pH was adjusted to 2 with hydrochloric acid.
Extract with stirring at room temperature overnight. The next morning, the mixture is filtered and passed through a column filled with 100 mL of Diaion SP825 resin. After passing through, wash with 300 mL of water, then 8
The adsorbed anthocyanidin glycoside is desorbed by flowing 400 mL of 0% ethanol. The desorbed solution is concentrated under reduced pressure and spray-dried to extract a blueberry extract.
86 g (total anthocyanidin content 35.20%) were obtained.

Example 3 400 L of water was added to 50 kg of frozen blueberry fruit (total anthocyanidin content 0.37%), and the pH was adjusted to pH 1 with hydrochloric acid. After adjusting to 5,
Extract at 40 ° C. with stirring overnight. The next morning, the mixture is filtered and passed through a column filled with 16 L of Diaion HP20 resin. After the passage, the plate is washed with 48 L of water, and then the adsorbed anthocyanidin glycoside is desorbed by flowing 64 L of 80% ethanol. The desorbed solution is decompressed and concentrated and spray-dried to obtain 505.00 g of a blueberry extract.
(Total anthocyanidin content 33.11%).

Example 4 200 g of frozen blueberry fruit (total anthocyanidin content 0.39%) was added to 8
Add 1000 mL of 0% ethanol and add sulfuric acid to pH2.
, And extract at room temperature with stirring overnight. After filtration the next morning, ethanol was distilled off by distillation, water was added, and the mixture was passed through a column filled with 100 mL of Diaion HP20 resin. After passing through, after washing with 300 mL of water,
The adsorbed anthocyanidin glycoside is desorbed by flowing 400 mL of 80% ethanol. The desorbed solution was concentrated under reduced pressure and spray-dried to obtain 1.90 g of a blueberry extract (total anthocyanidin content: 34.65%).

[0021]

According to the present invention, from blueberry fruits,
An extract having a high content of anthocyanidin glycoside can be efficiently extracted and can be used as various health foods.

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Claims (2)

[Claims] (1) using blueberry as a raw material, extracting with an acidic aqueous solution, an alcohol containing an acid or a hydroalcoholic; (2) solid-liquid separating the acidic extract;
(3) adsorbing the extract obtained by the solid-liquid separation on the synthetic adsorbent, desorbing the anthocyanidin glycoside from the synthetic adsorbent, and (4) concentrating and drying the desorbed liquid.
Manufacturing method of blueberry extract. 2. The method for producing a blueberry extract according to claim 1, wherein the pH of the acidic aqueous solution, the alcohol containing acid or the hydrous alcohol is 2 or less.