CN211785162U

CN211785162U - Microfluidic multifunctional semen analysis device

Info

Publication number: CN211785162U
Application number: CN201921606782.8U
Authority: CN
Inventors: 周小棉
Original assignee: Guangzhou City Biotron Biotechnology Co ltd
Current assignee: Guangzhou City Biotron Biotechnology Co ltd
Priority date: 2019-09-25
Filing date: 2019-09-25
Publication date: 2020-10-27
Anticipated expiration: 2029-09-25

Abstract

The utility model discloses a microfluidic multifunctional semen analysis device, which comprises an upper shell, a detection chip cover plate, a detection chip bottom plate and a lower shell, wherein the detection chip cover plate and the detection chip bottom plate are overlapped to form a microfluidic semen detection chip; the upper shell is provided with a sperm counting observation window and a pH detection window, the lower shell is provided with a light source entering window at the position corresponding to the sperm counting observation window, a sperm counting pool is arranged at the position corresponding to the sperm counting observation window in the microfluidic semen detection chip, a pH detection pool is arranged at the position corresponding to the pH detection window in the microfluidic semen detection chip, and pH test paper is placed in the pH detection pool. The device of the utility model is simple in structure, easy preparation, convenient to use not only can be used for clinical medical sperm inspection, can cooperate domestic sperm instrument on the market to get into the family moreover and use, can reduce the awkwardness of hospital's sample to a very big extent.

Description

Microfluidic multifunctional semen analysis device

Technical Field

The utility model relates to a novel medical treatment detects technical field, concretely relates to multi-functional semen analysis device of micro-fluidic.

Background

In recent years, the infertility rate worldwide has been as high as 15%, which is the third largest disease besides cancer and cardiovascular and cerebrovascular diseases. The global infertility couple case is about 6000-. Infertility can not only affect the work and life of patients, but also cause the problems of family rupture, social dissonance, reduction of the quality of the population at birth and the like. Semen analysis and detection is the standard for judging male fertility, and the detection method of sperm quality commonly used in clinical cases at present is computer-assisted sperm analysis (CASA). In the detection process, the preparation of a sample, the concentration of sperms, the depth of a counting cell, the gap of a counting plate, the parallelism of a glass slide and other practical operations and hardware problems can cause the inconsistency and the larger error of the quality detection of the sperms. Meanwhile, the CASA system is high in purchase cost, needs to be operated by professional technicians, and has strong subjectivity on diagnosis results of different doctors. Other detection methods such as optical turbidimetry, impedance analysis, and photon spectroscopy rely on expensive instruments and equipment, and the sample preparation and data analysis are complicated, which is contrary to the current development trend of rapid diagnosis in medical detection.

The microfluidic chip is originally called a 'Lab-on-a-chip' in the united states and called a 'micro-integrated analysis chip' in europe, and is a main platform for realizing microfluidic technology (Microfluidics), and can integrate basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes on a micron-scale chip to automatically complete the whole analysis process. The micro-fluidic chip has the advantages of light volume, small amount of used samples and reagents, high reaction speed, capability of massively parallel processing, disposability and the like, and has great potential in the fields of biology, chemistry, medicine and the like.

Because the methods such as CASA in clinic are expensive for sampling and detecting semen, complex in operation and damages the semen, and the requirements for the quality of professional medical personnel are high, a semen analysis and detection device which is convenient and easy to use, simple in operation and relatively low in cost is needed. The device not only can be used for the analysis and diagnosis of the quality of the clinical sperms, but also can be suitable for the self-operation detection of the pregnant women at home, thereby avoiding the embarrassment of sampling in hospitals. The method has the characteristics of high integration, small reagent dosage, microscale, quick response speed and sensitivity, and the microfluidic technology provides a new development platform for clinical and household semen detection and analysis.

SUMMERY OF THE UTILITY MODEL

The utility model provides a be not enough to prior art, the utility model provides a multi-functional semen analytical equipment of micro-fluidic can realize the multi-functional semen analytical equipment of micro-fluidic of quick sample and detection, this analytical equipment with semen count analysis and pH detection integration on same micro-fluidic chip, save operating procedure and cost, simple easy-to-use reduces the human error and disturbs.

In order to achieve the above purpose, the technical scheme of the utility model is that:

a microfluidic multifunctional semen analysis device comprises an upper shell, a detection chip cover plate, a detection chip base plate and a lower shell, wherein the detection chip cover plate and the detection chip base plate are stacked to form a microfluidic semen detection chip; the device comprises an upper shell, a microfluidic semen detection chip, a light source, a sperm counting and observing window, a pH detection pool and pH test paper, wherein the upper shell is provided with the sperm counting and observing window and the pH detection window, the lower shell is provided with a light source entering window at the position corresponding to the sperm counting and observing window, the microfluidic semen detection chip is internally provided with the sperm counting pool at the position corresponding to the sperm counting and observing window, the microfluidic semen detection chip is internally provided with the pH detection pool at the position corresponding to the pH detection window, and the pH test paper.

Furthermore, a semen inlet and a semen outlet are formed in the upper shell, a semen inlet hole and a semen outlet hole are further formed in the microfluidic semen detection chip, the semen inlet hole is communicated with the semen inlet and the sperm counting pool, and the semen outlet hole is communicated with the semen outlet and the sperm counting pool. So set up to can add the semen sample to the inside under the condition of not dismantling whole device, then further observe.

Furthermore, the microfluidic semen detection chip is also provided with a semen pH detection hole corresponding to the pH detection pool and a straight micro-channel for communicating the semen pH detection hole with the semen sampling hole.

Furthermore, the sperm counting cell is arranged on the top surface of the bottom plate of the detection chip, the semen inlet hole, the semen outlet hole and the semen pH detection hole are all arranged on the cover plate of the detection chip in a penetrating manner, and the straight micro-channel is arranged on the bottom surface of the cover plate of the detection chip. Set up like this to make straight microchannel at detection chip apron bottom surface, the semen counting cell is at the top surface that detects the chip film, and the two is located the both sides of chip composition face respectively, leads to the runner after forming to have certain difference in height, and the flow does not influence each other.

Further, a pH standard colorimetric area is arranged on the upper shell.

Further, the light source entrance window is a boss for restricting the divergence of the light source and reducing the intensity loss of the light source.

Further, still include the handle of taking of setting up in device one side.

Furthermore, the detection chip cover plate and the detection chip bottom plate are both made of transparent materials.

Furthermore, the detection chip cover plate and the detection chip bottom plate are combined and fixed in any one or more modes of gluing, ultrasonic sealing, hot-press bonding or laser sealing.

Furthermore, the upper shell and the lower shell are combined and fixed in any one or more modes of buckle fixation, screw fixation and adhesive fixation.

Compared with the prior art, the utility model, have following advantage:

the utility model discloses on integrating the sperm count function among the sperm quality analysis and semen pH detection function to same piece micro-fluidic chip, saved the procedure that the semen detected, reduced artificial operation error's interference. Meanwhile, by utilizing the specific structure, the flow channel of the pH detection pool and the flow channel of the semen counting pool are divided into a cover plate and a bottom plate which are positioned on the microfluidic semen detection chip, and have a certain height difference, so that the semen which is infected with the pH test paper in the sample introduction process is prevented from flowing back from the pH detection pool to the semen counting pool, and the cross contamination in the detection process is avoided.

The device of the utility model is simple in structure, easy preparation, convenient to use not only can be used for clinical medical sperm inspection, can cooperate domestic sperm instrument on the market to get into the family moreover and use, can reduce the awkwardness of hospital's sample to a very big extent.

Drawings

FIG. 1 is a schematic diagram of the external overall structure of a microfluidic multifunctional semen analysis device;

FIG. 2 is an exploded view of the internal structure of the microfluidic multifunctional semen analysis device;

FIG. 3 is a schematic diagram of a microfluidic semen detection chip of the microfluidic multifunctional semen analysis device;

description of reference numerals: 1. a semen detection observation area; 2. taking a handle; 3. an upper housing; 4. detecting a chip cover plate; 5. detecting a chip film; 6. a lower housing; 7. a semen outlet; 8. a sperm counting observation window; 9. a semen injection port; 10. a pH detection window; 11. a pH standard colorimetric region; 12. semen outlet holes; 13. a semen sampling hole; 14. a semen pH detection hole; 15. pH test paper; 16. a sperm counting pool; 17. a pH detection pool; 18. a light source enters the window; 19. a straight microchannel; 20. microfluidic semen detection chip.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

Examples

As shown in figure 1, the microfluidic multifunctional semen analysis device comprises an upper shell 3, a detection chip cover plate 4, a detection chip base plate 5 and a lower shell 6, wherein the detection chip cover plate 4 and the detection chip base plate 5 are overlapped to form a microfluidic semen detection chip 20, the upper shell 3, the microfluidic semen detection chip 20 and the lower shell 6 are sequentially overlapped and combined to form the whole analysis device, and the whole analysis device is divided into a semen detection observation area 1 and a taking handle 2.

As shown in fig. 2 and fig. 3, a sperm counting cell 16 and a pH detection cell 17 are arranged in the microfluidic semen detection chip 20, the sperm counting cell 16 and the pH detection cell 17 are both arranged on the detection chip substrate 5, pH test paper 15 is placed in the pH detection cell 17, a semen inlet hole 13 and a semen outlet hole 12 are arranged on the detection chip cover 4 in a penetrating manner, and the semen inlet hole 13 is communicated to the sperm counting cell 16. Meanwhile, a semen pH detection hole 14 is formed in the detection chip cover plate 4 in a penetrating mode, a straight micro-channel 19 is formed in the bottom surface of the detection chip cover plate 4, and the straight micro-channel 19 is used for communicating the semen pH detection hole 14 with the semen sampling hole 13, so that a semen sample can flow into the pH detection pool 17 and the sperm counting pool 16 simultaneously when entering from the semen sampling hole 13. The whole microfluidic semen detection chip 20 is made of a non-toxic transparent material, such as transparent glass or quartz, or other non-toxic plastic transparent materials, such as one of polydimethylsiloxane, polymethyl methacrylate, polystyrene, polycarbonate or cyclic olefin copolymer.

In this way, the straight micro-channel 19 is disposed on the bottom surface of the cover plate 4 of the detection chip, and the sperm counting cell 16 is disposed on the top surface of the bottom plate 5 of the detection chip, so that the two are respectively disposed on two sides of the bonding surface of the chip, resulting in a certain height difference between the formed flow channels, and the flows are not affected by each other.

As shown in fig. 2, the upper shell 3 is provided with a sperm counting observation window 8 and a pH detection window 10, the lower shell 6 is provided with a light source entrance window 18, and the positions of the sperm counting observation window 8 and the light source entrance window 18 are correspondingly arranged at the position of the sperm counting cell 16 for observing the condition of the sample in the sperm counting cell 16; the position of the pH detection window 10 is correspondingly arranged at the position of the pH detection pool 17 and is used for observing the color change condition of the sample in the pH detection pool 17, and a pH standard colorimetric region 11 is also arranged near the position of the pH detection window 10 so that a user can compare and know the condition of the sample; the light source entrance window 18 on the lower housing 6 is a boss design, the purpose of which is to restrict the divergence of the light source as much as possible and to reduce the intensity loss of the light source.

Meanwhile, the upper shell 3 is provided with a semen inlet 9 and a semen outlet 7, the positions of the upper shell correspond to the positions of the semen inlet hole 13 and the semen outlet 7, and the upper shell 3 is communicated with a sperm counting cell 16 in the internal microfluidic semen detection chip 20 through the semen inlet 9 and the semen outlet 7, so that a sample can be added and put in without a disassembling device.

Specifically, the detection chip cover plate 4 and the detection chip bottom plate 5 are combined and fixed in any one or more modes of gluing, ultrasonic sealing, hot-press bonding or laser sealing; the upper shell 3 and the lower shell 6 are combined and fixed in any one or more modes of buckle fixation, screw fixation and adhesive fixation. Therefore, the position matching relationship among all parts is ensured, and the integral device is not required to be disassembled when a sample is added, so that the use of the integral device is facilitated.

When the device is used, a semen sample enters the semen inlet 13 on the corresponding microfluidic semen detection chip 20 below through the semen inlet 9, and respectively flows into the semen counting cell 16 on the bottom plate of the chip and flows into the pH detection cell 17 through the straight micro-channel 19 on the cover plate 4 of the detection chip under the action of gravity.

After the semen sample flows into the sperm counting pool 16, because the semen has certain viscosity, after the semen sample is fully paved in the sperm counting pool 16, redundant samples sequentially flow through the semen outlet hole 12 and the semen outlet 7. The semen sample dispersed in the sperm cell 16 can be visually observed for its overall apparent color and consistency.

The other side of the solution flows through the straight micro-channel 19 and reaches the pH detection pool 17, and after the other side of the solution reacts with the pH test paper 15 in the pH detection pool, the pH test paper 15 can show different colors along with the difference of the pH value of the solution, namely, the color reaction is generated. After the color reaction occurs, the pH test paper 15 changes color, the color is observed from the pH detection window 10, and the pH value of the semen sample is obtained by comparing the pH value with the pH standard color comparison area 11. Because the straight micro-channel 19 is positioned on the reverse side of the detection chip cover plate 4 and has a certain height difference with the flow channel of the sperm counting pool 16 on the detection chip bottom plate 5, the straight micro-channel and the flow channel are not positioned on the same plane, the influence of backflow can be avoided during flowing, and the interference of the pollution of the pH test paper 15 on sperm counting is reduced.

The utility model discloses a miniflow channel based on among the micro-fluidic chip detects and observes, and the sample volume is little when the semen detects, can cooperate clinical and domestic detecting instrument to use. When the light source is used with an instrument, the light source enters the window from the lower bottom shell and is designed as a boss, so that the divergence of the light source is restrained as much as possible, and the intensity loss of the light source is reduced. The microfluidic detection chip is made of transparent material, so that the light source can illuminate the sperm counting cell through the microfluidic detection chip, the brightness intensity of the counting cell is increased, and the precision of the instrument in detecting and counting through the semen counting observation window is improved. The utility model discloses a cooperation instrument carries out accurate count calculation to the sperm in the chip counting cell to can analyze the survival rate of sperm, the velocity of motion, the deformity rate etc. carries out comprehensive accurate analysis to the sperm quality.

The utility model discloses a multi-functional semen analytical equipment of micro-fluidic based on the design of micro-fluidic chip, semen sample quantity when not only can reducing sperm quality analysis, in addition with integrated to same micro-fluidic chip of sperm count function and semen pH detection function, simple structure, convenient operation can avoid the cross contamination in the testing process simultaneously.

The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, which cannot limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.

Claims

1. A microfluidic multifunctional semen analysis device is characterized in that: the microfluidic semen detection device comprises an upper shell (3), a detection chip cover plate (4), a detection chip bottom plate (5) and a lower shell (6), wherein the detection chip cover plate (4) and the detection chip bottom plate (5) are overlapped to form a microfluidic semen detection chip (20), and the upper shell (3), the microfluidic semen detection chip (20) and the lower shell (6) are sequentially overlapped and combined to form an integral analysis device; go up and be equipped with sperm count observation window (8) and pH detection window (10) on shell (3), the position department that corresponds with sperm count observation window (8) is equipped with light source entering window (18) down on shell (6), the position that corresponds with sperm count observation window (8) in micro-fluidic semen detection chip (20) is equipped with sperm count pond (16), the position that corresponds with pH detection window (10) in micro-fluidic semen detection chip (20) is equipped with pH detection pond (17), pH test paper (15) have been put in pH detection pond (17).

2. The microfluidic multifunctional semen analysis device of claim 1, wherein: go up and seted up semen introduction port (9) and semen outlet (7) on shell (3), it advances appearance hole (13) and semen outlet (12) still to have seted up semen on micro-fluidic semen detection chip (20), semen introduction hole (13) intercommunication semen introduction port (9) and sperm count pool (16), semen outlet (12) intercommunication semen outlet (7) and sperm count pool (16).

3. The microfluidic multifunctional semen analysis device of claim 2, wherein: the microfluidic semen detection chip (20) is also provided with a semen pH detection hole (14) corresponding to the pH detection pool (17) and a straight micro-channel (19) for communicating the semen pH detection hole (14) with the semen sampling hole (13).

4. The microfluidic multifunctional semen analysis device of claim 3, wherein: the sperm counting cell (16) is arranged on the top surface of the detection chip bottom plate (5), the semen sample inlet hole (13), the semen sample outlet hole (12) and the semen pH detection hole (14) are arranged on the detection chip cover plate (4) in a penetrating mode, and the straight micro-channel (19) is arranged on the bottom surface of the detection chip cover plate (4).

5. The microfluidic multifunctional semen analysis device of claim 1, wherein: and a pH standard colorimetric region (11) is also arranged on the upper shell (3).

6. The microfluidic multifunctional semen analysis device of claim 1, wherein: the light source entry window (18) is a boss for constraining divergence of the light source and reducing intensity loss of the light source.

7. The microfluidic multifunctional semen analysis device of claim 1, wherein: also comprises a taking handle (2) arranged on one side of the device.

8. The microfluidic multifunctional semen analysis device of claim 1, wherein: the detection chip cover plate (4) and the detection chip bottom plate (5) are both made of transparent materials.

9. The microfluidic multifunctional semen analysis device of claim 1, wherein: the detection chip cover plate (4) and the detection chip bottom plate (5) are combined and fixed in any one or more modes of gluing, ultrasonic sealing, hot-press bonding or laser sealing.

10. The microfluidic multifunctional semen analysis device of claim 1, wherein: the upper shell (3) and the lower shell (6) are combined and fixed in any one or more modes of buckle fixation, screw fixation and adhesive fixation.