CN110879226B - Microfluidic multifunctional semen analysis device - Google Patents
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- CN110879226B CN110879226B CN201910912009.2A CN201910912009A CN110879226B CN 110879226 B CN110879226 B CN 110879226B CN 201910912009 A CN201910912009 A CN 201910912009A CN 110879226 B CN110879226 B CN 110879226B
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- 238000009612 semen analysis Methods 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 133
- 210000000582 semen Anatomy 0.000 claims abstract description 107
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 238000004458 analytical method Methods 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
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- 230000000149 penetrating effect Effects 0.000 claims description 4
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- 238000005070 sampling Methods 0.000 abstract description 6
- 238000007689 inspection Methods 0.000 abstract description 2
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- 238000006243 chemical reaction Methods 0.000 description 3
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- 238000003745 diagnosis Methods 0.000 description 2
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- 231100000252 nontoxic Toxicity 0.000 description 2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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Abstract
The invention discloses a microfluidic multifunctional semen analysis device, which comprises an upper shell, a detection chip cover plate, a detection chip negative plate and a lower shell, wherein the detection chip cover plate and the detection chip negative plate are overlapped to form a microfluidic semen detection chip; the upper shell is provided with a sperm counting observation window and a pH detection window, the lower shell is provided with a light source entering window at the position corresponding to the sperm counting observation window, a sperm counting pool is arranged at the position corresponding to the sperm counting observation window in the microfluidic semen detection chip, a pH detection pool is arranged at the position corresponding to the pH detection window in the microfluidic semen detection chip, and pH test paper is arranged in the pH detection pool. The device has simple structure, easy manufacture and convenient use, not only can be used for clinical medical sperm inspection, but also can be matched with household sperm instruments on the market to enter home for use, and can greatly reduce the embarrassment of hospital sampling.
Description
Technical Field
The invention relates to the technical field of novel medical detection, in particular to a microfluidic multifunctional semen analysis device.
Background
Semen analysis and detection is a standard for judging male fertility, and a commonly used method for detecting sperm quality in clinical cases is computer-aided sperm analysis (CASA). In the detection process, the problems of actual operations and hardware such as sample preparation, sperm concentration, counting cell depth, counting plate clearance, slide parallelism and the like can cause non-uniformity and larger error of sperm quality detection. Meanwhile, the CASA system has high purchase cost, needs operation by professional technical staff, and has strong subjectivity of diagnosis results of different doctors. Other detection methods such as a light turbidity method, an impedance analysis method, a photon spectrometry method and the like are mostly dependent on expensive instruments and equipment, and the sample preparation and data analysis are complex, which is contrary to the development trend of the rapid diagnosis of the current medical detection.
Microfluidic chips, originally called "Lab-on-a-chips" in the united states and "micro-integrated analytical chips" (Micrototal ANALYTICAL SYSTEMS) in europe, are the main platform for microfluidic technology (Microfluidics) implementation, and can integrate basic operation units of sample preparation, reaction, separation, detection, etc. in biological, chemical and medical analysis processes onto a chip with a micrometer scale, so as to automatically complete the whole analysis process. The microfluidic chip has the advantages of light volume, small amount of used sample and reagent, high reaction speed, capability of parallel processing in a large amount, capability of being disposable, and the like, and has great potential in the fields of biology, chemistry, medicine, and the like.
As methods such as clinical CASA and the like are expensive for semen sampling and detection, the operation is complex, the damage to sperms is caused, and the quality requirements on professional medical staff are high, the semen analysis and detection device which is convenient and easy to use, simple to operate and relatively low in cost is needed. The device not only can be used for analyzing and diagnosing the quality of clinical sperms, but also can be suitable for self-operation detection of family pregnant couples, and the embarrassment of hospital sampling is avoided. Can be highly integrated, has small reagent consumption, micro-scale, rapid and sensitive response speed, the microfluidic technology provides a new developable platform for clinical and household semen detection and analysis.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a microfluidic multifunctional semen analysis device which can realize rapid sampling and detection, the analysis device integrates semen counting analysis and pH detection on the same microfluidic chip, saves operation procedures and cost, is simple and easy to use, and reduces human error interference.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
The microfluidic multifunctional semen analysis device comprises an upper shell, a detection chip cover plate, a detection chip bottom plate and a lower shell, wherein the detection chip cover plate and the detection chip bottom plate are overlapped to form a microfluidic semen detection chip, and the upper shell, the microfluidic semen detection chip and the lower shell are sequentially overlapped and combined to form an integral analysis device; the upper shell is provided with a sperm counting observation window and a pH detection window, a light source entering window is arranged at the position corresponding to the sperm counting observation window on the lower shell, a sperm counting pool is arranged at the position corresponding to the sperm counting observation window in the microfluidic semen detection chip, a pH detection pool is arranged at the position corresponding to the pH detection window in the microfluidic semen detection chip, and pH test paper is arranged in the pH detection pool.
Further, a semen sample inlet and a semen sample outlet are formed in the upper shell, a semen sample inlet hole and a semen sample outlet hole are also formed in the microfluidic semen detection chip, the semen sample inlet hole is communicated with the semen sample inlet and the semen counting pool, and the semen sample outlet hole is communicated with the semen sample outlet and the semen counting pool. This arrangement allows for the addition of semen samples to the interior for further observation without disassembling the overall device.
Further, the microfluidic semen detection chip is also provided with a semen pH detection hole at a position corresponding to the pH detection pool and a straight micro-channel for communicating the semen pH detection hole and the semen sample injection hole.
Further, the sperm counting pool is arranged on the top surface of the bottom sheet of the detection chip, the semen sample injection hole, the semen sample outlet hole and the semen pH detection hole are all arranged on the cover sheet of the detection chip in a penetrating way, and the straight micro-channel is arranged on the bottom surface of the cover sheet of the detection chip. The arrangement is so that the straight micro-channel is arranged on the bottom surface of the cover plate of the detection chip, the semen counting pool is arranged on the top surface of the bottom plate of the detection chip, and the straight micro-channel and the semen counting pool are respectively arranged on two sides of the joint surface of the chip, so that the formed flow channels have a certain height difference, and the flow cannot affect each other.
Further, the upper shell is also provided with a pH standard specific color zone.
Further, the light source entering window is a boss for restraining divergence of the light source and reducing intensity loss of the light source.
Further, the device also comprises a taking handle arranged at one side of the device.
Further, the detection chip cover plate and the detection chip bottom plate are both made of transparent materials.
Further, the detection chip cover plate and the detection chip are combined and fixed in any one or more modes of gluing, ultrasonic sealing, thermocompression bonding or laser sealing.
Further, the upper shell and the lower shell are combined and fixed in any one or more modes of fastening, screw fixing and adhesive fixing.
Compared with the prior art, the invention has the following advantages:
According to the invention, the sperm counting function and the semen pH detection function in sperm quality analysis are integrated on the same microfluidic chip, so that the semen detection program is saved, and the interference of artificial operation errors is reduced. Meanwhile, by utilizing the specific structure, the pH detection pool flow channel and the semen counting pool flow channel are respectively arranged on the cover plate and the bottom plate of the microfluidic semen detection chip, have a certain height difference, and prevent semen stained with pH test paper in the sample injection process from flowing back into the semen counting pool from the pH detection pool, thereby avoiding cross contamination in the detection process.
The device has simple structure, easy manufacture and convenient use, not only can be used for clinical medical sperm inspection, but also can be matched with household sperm instruments on the market to enter home for use, and can greatly reduce the embarrassment of hospital sampling.
Drawings
FIG. 1 is a schematic diagram of the external overall structure of a microfluidic multifunctional semen analysis device;
FIG. 2 is an exploded schematic view of the internal structure of the microfluidic multifunctional semen analysis device;
FIG. 3 is a schematic diagram of a microfluidic semen detection chip of the microfluidic multifunctional semen analysis device;
reference numerals illustrate: 1. a semen detection observation area; 2. a handle is taken; 3. an upper housing; 4. detecting a chip cover plate; 5. detecting a chip negative film; 6. a lower housing; 7. semen outlet; 8. a sperm count viewing window; 9. a semen sample inlet; 10. a pH detection window; 11. a pH standard ratio zone; 12. semen sample outlet; 13. semen injection holes; 14. semen pH detection well; 15. a pH test paper; 16. a sperm counting cell; 17. a pH detection cell; 18. the light source enters the window; 19. a straight microchannel; 20. microfluidic semen detection chip.
Detailed Description
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Examples
As shown in fig. 1, a microfluidic multifunctional semen analysis device comprises an upper shell 3, a detection chip cover plate 4, a detection chip bottom plate 5 and a lower shell 6, wherein the detection chip cover plate 4 and the detection chip bottom plate 5 are overlapped to form a microfluidic semen detection chip 20, and the upper shell 3, the microfluidic semen detection chip 20 and the lower shell 6 are sequentially overlapped and combined to form an integral analysis device, and the integral analysis device is divided into a semen detection observation area 1 and a taking handle 2.
As shown in fig. 2 and 3, a sperm counting pool 16 and a pH detection pool 17 are arranged in the microfluidic semen detection chip 20, the sperm counting pool 16 and the pH detection pool 17 are both arranged on the bottom sheet 5 of the detection chip, a pH test paper 15 is placed in the pH detection pool 17, a semen sample injection hole 13 and a semen sample outlet hole 12 are arranged on the cover sheet 4 of the detection chip in a penetrating way, and the semen sample injection hole 13 is communicated into the sperm counting pool 16. Meanwhile, the semen pH detection hole 14 is formed in the detection chip cover plate 4 in a penetrating manner, the straight micro-channel 19 is formed in the bottom surface of the detection chip cover plate 4 and is used for communicating the semen pH detection hole 14 with the semen sample injection hole 13, and therefore semen samples can flow into the pH detection pool 17 and the sperm counting pool 16 simultaneously when entering from the semen sample injection hole 13. The whole microfluidic semen detection chip 20 is made of nontoxic transparent materials, and can be transparent glass or quartz, or can be made of other nontoxic plastic transparent materials, such as one of polydimethylsiloxane, polymethyl methacrylate, polystyrene, polycarbonate or cycloolefin copolymer.
In this way, the straight micro-channel 19 is arranged on the bottom surface of the detection chip cover plate 4, and the sperm counting pool 16 is arranged on the top surface of the detection chip bottom plate 5, so that the two are respectively positioned on two sides of the chip joint surface, a certain height difference exists in the formed flow channel, and the flow is not affected.
As shown in fig. 2, the upper shell 3 is provided with a sperm counting observation window 8 and a pH detection window 10, the lower shell 6 is provided with a light source entering window 18, and the positions of the sperm counting observation window 8 and the light source entering window 18 are correspondingly arranged at the position of the sperm counting cell 16 for observing the condition of a sample in the sperm counting cell 16; the position of the pH detection window 10 is correspondingly arranged at the position of the pH detection pool 17 and is used for observing the color change condition of a sample in the pH detection pool 17, and a pH standard ratio color zone 11 is arranged at the position of the pH detection window 10 in an accessory manner so as to facilitate the comparison of a user to know the condition of the sample; the light source entrance window 18 on the lower housing 6 is of a boss design, which aims to restrict the divergence of the light source as much as possible and reduce the light source intensity loss.
Meanwhile, the upper shell 3 is provided with the semen sample inlet 9 and the semen sample outlet 7, the positions of the semen sample inlet 9 and the semen sample outlet 7 correspond to the positions of the semen sample inlet 13 and the semen sample outlet 7, and the semen sample inlet 9 and the semen sample outlet 7 arranged on the upper shell 3 are communicated with the semen counting pond 16 in the internal microfluidic semen detection chip 20, so that the sample can be added and put into without disassembling the device.
Specifically, the detection chip cover plate 4 and the detection chip 5 are combined and fixed by adopting any one or more modes of gluing, ultrasonic sealing, hot-press bonding or laser sealing; the upper shell 3 and the lower shell 6 are combined and fixed in any one or a plurality of modes of buckling fixation, screw fixation and adhesive fixation. Therefore, the position matching relation among the parts is ensured, and the whole device is not required to be disassembled when the sample is added, so that the use of the whole device is facilitated.
Specifically, when in use, semen sample passes through the semen injection port 9 and enters the semen injection hole 13 on the microfluidic semen detection chip 20 corresponding to the lower part, and under the action of gravity, flows into the semen counting pool 16 on the chip bottom sheet and flows into the pH detection pool 17 through the straight micro-channel 19 on the detection chip cover sheet 4.
After the semen sample flows into the semen counting cell 16, the semen sample is paved on the semen counting cell 16 due to certain viscosity, and redundant samples sequentially flow out from the semen sample outlet 7 through the semen sample outlet 12. The semen sample dispersed in the sperm count cell 16 may visually observe its overall apparent color and consistency.
The other side of the solution flows through the straight micro-channel 19 and then reaches the pH detection tank 17, and after the solution reacts with the pH test paper 15 in the solution, the pH test paper 15 can show different colors according to the pH value of the solution, namely, a color reaction occurs. After the color reaction, the pH test paper 15 changes color, the color is observed from the pH detection window 10, and the pH value of the semen sample is obtained by comparing the pH standard ratio color area 11. Because the straight micro-channel 19 is positioned on the back surface of the detection chip cover plate 4 and is different from the flow channel of the sperm counting pool 16 on the detection chip bottom plate 5 by a certain height, the two are not on the same plane, the influence of backflow can be avoided during flowing, and the interference of the pollution of the pH test paper 15 on sperm counting is reduced.
The invention is based on the micro-flow channel in the micro-flow control chip for detection and observation, has small sampling amount during semen detection, and can be matched with clinical and household detection instruments for use. When the light source is matched with an instrument, the light source enters from the light source entering window on the lower bottom shell, the light source entering window is designed as a boss, the divergence of the light source is restrained as much as possible, and the light source intensity loss is reduced. The micro-fluidic detection chip is made of transparent material, so that the light source can illuminate the sperm counting pool through the micro-fluidic detection chip, the brightness intensity of the counting pool is increased, and the accuracy of the instrument in detecting and counting through the sperm counting observation window is improved. The invention is matched with an instrument to accurately count and calculate the sperms in the chip counting pool, and can analyze the survival rate, the movement speed, the deformity rate and the like of the sperms and comprehensively and accurately analyze the quality of the sperms.
The microfluidic multifunctional semen analysis device disclosed by the invention is based on the design of the microfluidic chip, not only can reduce the semen sample consumption in the process of sperm quality analysis, but also integrates the sperm counting function and the semen pH detection function on the same microfluidic chip, has a simple structure, is convenient to operate, and can avoid cross contamination in the detection process.
The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement the same, and are not intended to limit the scope of the present invention. All equivalent changes or modifications made in accordance with the essence of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. A microfluidic multifunctional semen analysis device is characterized in that: the microfluidic semen detection device comprises an upper shell (3), a detection chip cover plate (4), a detection chip bottom plate (5) and a lower shell (6), wherein the detection chip cover plate (4) and the detection chip bottom plate (5) are overlapped to form a microfluidic semen detection chip (20), and the upper shell (3), the microfluidic semen detection chip (20) and the lower shell (6) are sequentially overlapped and combined to form an integral analysis device; the sperm counting device is characterized in that a sperm counting observation window (8) and a pH detection window (10) are arranged on the upper shell (3), a light source entering window (18) is arranged at a position, corresponding to the sperm counting observation window (8), of the lower shell (6), a sperm counting pool (16) is arranged at a position, corresponding to the sperm counting observation window (8), of the microfluidic semen detection chip (20), a pH detection pool (17) is arranged at a position, corresponding to the pH detection window (10), of the microfluidic semen detection chip (20), pH test paper (15) is arranged in the pH detection pool (17), and the pH detection pool (17) is arranged on a detection chip negative film (5);
The upper shell (3) is provided with a semen sample inlet (9) and a semen sample outlet (7), the microfluidic semen detection chip (20) is also provided with a semen sample inlet (13) and a semen sample outlet (12), the semen sample inlet (13) is communicated with the semen sample inlet (9) and the semen counting pool (16), and the semen sample outlet (12) is communicated with the semen sample outlet (7) and the semen counting pool (16);
the upper shell (3) is also provided with a pH standard specific color zone (11);
The microfluidic semen detection chip (20) is also provided with a semen pH detection hole (14) at a position corresponding to the pH detection pool (17), and a straight micro-channel (19) for communicating the semen pH detection hole (14) with the semen sample injection hole (13);
The sperm counting pool (16) is arranged on the top surface of the detection chip negative film (5), the semen sample injection hole (13), the semen sample outlet hole (12) and the semen pH detection hole (14) are all arranged on the detection chip cover plate (4) in a penetrating way, and the straight micro-channel (19) is arranged on the bottom surface of the detection chip cover plate (4);
the light source entrance window (18) is a boss for restricting divergence of the light source and reducing loss of light source intensity.
2. The microfluidic multifunctional semen analysis device according to claim 1, wherein: the device also comprises a taking handle (2) arranged at one side of the device.
3. The microfluidic multifunctional semen analysis device according to claim 1, wherein: the detection chip cover plate (4) and the detection chip bottom plate (5) are both made of transparent materials.
4. The microfluidic multifunctional semen analysis device according to claim 1, wherein: the detection chip cover plate (4) and the detection chip bottom plate (5) are combined and fixed in any one or more modes of gluing, ultrasonic sealing, hot-press bonding or laser sealing.
5. The microfluidic multifunctional semen analysis device according to claim 1, wherein: the upper shell (3) and the lower shell (6) are fixed in a combined way by adopting any one or more modes of buckling fixation, screw fixation and adhesive fixation.
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| CN201910912009.2A CN110879226B (en) | 2019-09-25 | 2019-09-25 | Microfluidic multifunctional semen analysis device |
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| CN201910912009.2A CN110879226B (en) | 2019-09-25 | 2019-09-25 | Microfluidic multifunctional semen analysis device |
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| CN110879226B true CN110879226B (en) | 2024-09-17 |
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| CN211785162U (en) * | 2019-09-25 | 2020-10-27 | 广州市宝创生物技术有限公司 | Microfluidic multifunctional semen analysis device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8778279B2 (en) * | 2009-07-06 | 2014-07-15 | Sony Corporation | Microfluidic device |
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| CN107099444A (en) * | 2017-04-17 | 2017-08-29 | 隗慧林 | Sperm sorting chip, sperm detection device and sperm detection method |
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| CN109900889A (en) * | 2019-03-22 | 2019-06-18 | 北京乐普医疗科技有限责任公司 | A kind of three detection cards of automatic hemofiltration dry type photochemical method microchannel liver function |
| CN211785162U (en) * | 2019-09-25 | 2020-10-27 | 广州市宝创生物技术有限公司 | Microfluidic multifunctional semen analysis device |
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