CN208829688U - A kind of electric shock pipe - Google Patents
A kind of electric shock pipe Download PDFInfo
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- CN208829688U CN208829688U CN201821151341.9U CN201821151341U CN208829688U CN 208829688 U CN208829688 U CN 208829688U CN 201821151341 U CN201821151341 U CN 201821151341U CN 208829688 U CN208829688 U CN 208829688U
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- electric shock
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Abstract
The utility model provides a kind of electric shock pipe, including tube body (1), pipe lid (2) and pin electrode array (3), has the cavity (5) for accommodating target liq sample inside the tube body (1).The electric shock pipe of the utility model, direction of an electric field is vertical with tube body, and effective electric field can be formed by filling it up with without liquid, and inside cavity not will form bubble and lead to electrode loose contact, can avoid electrode loose contact influence electricity and turns effect.
Description
Technical field
The utility model belongs to electrotransfection technical field, is related to a kind of electric shock pipe, is specifically related to one kind and is provided with needle electricity
The electric shock pipe of pole array.
Background technique
Since the 1970's electroporation technology just be used to for molecule importeding into zooblast or plant cell, researcher
It confirms, cell, which is exposed to, can make to form channel on after birth in of short duration lasting high voltage electric field, and macromolecular such as protein and DNA can lead to
The channel is crossed to enter into the cell.Electroporation be it is a kind of be widely used and highly recommended for test cell line and gene therapy
Method.When applying strong electrical field, cell membrane is temporarily changed to porous property and can be through foreign material, such as macromolecular.
Cell membrane electricity penetration depends on the various parameters of electric field, such as pulse pattern, pulse voltage, pulse duration, pulse
Quantity and other experiment conditions.
Equipment currently used for cell electrotransfection mainly has electric shock cup.
Describe a kind of electric shock pipe in the patent of US20130052711A1, effect be added in pipe containing cell and
Top electrode and lower electrode is respectively set in the upper/lower terminal of electric shock pipe, by thin in the liquid sample that the substance of cell will be injected
Born of the same parents' electroporation power supply is connected with top electrode and lower electrode, to make to form electric field in electric shock pipe, and then makes extracellular substance
Into intracellular.And after filling in liquid sample in electric shock pipe, before starting electric shock, need to prevent from having time in the pipe that shocks by electricity
Gas remains to form aeration electrical connection.In that patent, applicant has one and electric shock in design on the nozzle outer wall of electric shock pipe
The annular groove that tube chamber is connected, the sustainable injection liquid sample into electric shock tube chamber of experimenter is until liquid surface protrudes
After electric shock tube chamber, then it will power on and be contacted with liquid convex surface until being pressed onto the upper edge of electric shock tube chamber, formation is to cavity under polar cap
The sealing of the inside liquid, and the liquid overflowed on a small quantity flows in annular groove.This design can generally prevent from having in electric shock pipe
Residual air influences electrical connection.
But find in use, the electric shock pipe in above-mentioned patent is relatively high to the precise requirements of manufacture, tests simultaneously
The requirement of person's operation is also relatively high.If upper inaccurate in manufacture, it is easy to cause the poor sealing on edge on electrode and cavity,
May under electrode cap after electrode occur upwarp to leave on cavity edge again outside air is caused to reenter cavity.
In addition if operator's improper use when is also easy to cause in pipe and has residual air bubble, the effect of cell electrotransfection is influenced
Fruit.Scientific experiment is very high to the reliability requirement of instrument and equipment, and therefore, above-mentioned electric shock pipe needs increase some mention in manufacture
The design of high reliability, to improve the reliability of experimenter's operation.
Chinese patent CN201410722470 provides a kind of electric shock pipe and the cell electrotransfection instrument with electric shock pipe, belongs to
Biological medicine instrument and equipment technical field.The electric shock pipe includes tube body, first electrode, second electrode and chock plug, tubular body tool
There is a cavity for accommodating target liq sample, one end of tube body is equipped with first electrode, and second electrode is set in pipe lid and the
The outer end of two electrodes can be electrically connected by the opening of pipe lid with outside, and the elasticity being connected with second electrode is provided in pipe lid
The outside of part, elastic component is connect with pipe lid, and the inside of elastic component is connected with second electrode.Can effectively improve second electrode with
Sealing performance between opening, so that the air extraneous when fluid sample is added be avoided to enter in cavity.
But at present there is following technical problem in the electric shock pipe when in use:
First is that the treating capacity of electrotransfection has to be larger than the capacity of cavity every time, causing to waste, cell liquid must overflow cavity,
If cell liquid is less than the volume of cavity, it will lead to second electrode and do not reach liquid, electrical connection cannot be formed.
Second is that inside cavity forms bubble, and bubble can not exclude in time in electrotransfection, bubble is attached to the second electricity
On extremely, electrotransfection effect is influenced
Third is that the electric shock pipe is when in use, it is necessary to be handled using high-voltage pulse, be easy to produce electric arc, influence operator's peace
Entirely.
Summary of the invention:
In order to solve the above-mentioned technical problem, it is approximate to provide a kind of surface structure for the utility model, but uses high density point
Cloth stereo electrod replaces the electric shock pipe device of first electrode and second electrode.
Technical solution provided by the utility model is: a kind of electric shock pipe, including tube body, pipe lid and pin electrode array, it is described
Tubular body has the cavity for accommodating target liq sample, which is characterized in that one end of the tube body seals, the tube body
The other end there is the opening that is connected with the cavity, the working position of the pin electrode array is placed in the cavity, institute
The edge for stating opening has the end face of annular；The pin electrode array is set in the pipe lid, and can pass through the outer of pipe lid
Portion's opening is electrically connected with outside, and the inner face of the pipe lid can have the end face of annular is fixed to connect with the edge of body open
It connects, printed circuit board is arranged in the external of the pipe lid.
It is preferred that the pin electrode array includes multiple pin electrodes, it is temporally all on each electrode in the multiple pin electrode
Phase applies the first polar electric pulse and the second polar electric pulse respectively, wherein the first polar electric pulse is corresponding
What is be distributed around electrode is all the corresponding electrode of the second polar electric pulse, and first polarity and the second polarity are opposite pole
Property.
It is preferred that multiple pin electrodes in the pin electrode array are arranged by regular polygon, it is adjacent in the pin electrode array
The distance between two electrodes it is equal.
It is preferred that the shape of the pin electrode array is the regular hexagon being made of several equilateral triangles, the electrode position
In on the vertex of the equilateral triangle.
It is preferred that first polarity is anode, second polarity is cathode.
It is preferred that the diameter of the electrode is 0.01-1.2 millimeters, the distance between described two adjacent electrode central points are
0.1-2.4 millimeters, the number of the electrode is greater than 19, and the material of the electrode is including but not limited to stainless steel.
It is preferred that the length of the pin electrode array in the cavity is identical as the length of the cavity.
It is respectively set on the outside of annular end face it is preferred that the inner face of the pipe lid and the edge of the body open have
Screw thread, the tube body are threadably secured with the pipe lid and connect.
It is preferred that at least one circle groove of outside setting of the end face of the annular, the inner face setting of the pipe lid are corresponding
Bulge-structure, the bulge-structure is corresponding with the groove of the outside of the end face of annular setting, and forms a fixed connection.
It is preferred that grab is arranged on the tube body, the pipe covers the buckle that setting can be mutually clamped with the grab.
It is preferred that the pipe covers setting grab, the buckle that can be mutually clamped with the grab is set on the tube body.
It is preferred that the inner face internal diameter of the pipe cover gab is less than the outer diameter of body open edge annular end face.
It is preferred that the inner face internal diameter of the pipe cover gab is equal to the outer diameter of body open edge annular end face.
It is preferred that the pipe lid is connect with tube body by flexibility of linking rod.
It is prepared it is preferred that the tube body and Guan Gai are all made of the integrally formed mode of mold.
The beneficial technical effect of the utility model:
The electric shock pipe of the utility model, with the structure provided according to CN201410722470 compared with similar electric shock pipe, electricity
Field direction is vertical with tube body, and effective electric field can be formed by filling it up with without liquid, and inside cavity not will form bubble, can avoid because of liquid
Body is less than or bubble causes to lose electrical connection between electrode or electrical connection poor contact influence electricity turns effect.
Detailed description of the invention
Fig. 1 electric shock pipe overall structure diagram described in the utility model；
Fig. 2 electric shock pipe overall structure diagram described in the utility model；
Attached drawing annotation: 1- tube body, 2- pipe lid, 3- pin electrode array, 4- printed circuit board, 5- cavity, 6- groove, 7- protrusion
Structure, 8- external screw thread, 9- internal screw thread.
Specific embodiment
The preferred embodiment of the utility model is described in detail with reference to the accompanying drawing, so that the advantages of the utility model
It can be easier to be readily appreciated by one skilled in the art with feature, to make the protection scope of the utility model apparent clear
Define.
Embodiment one
Electric shock pipe provided by the utility model is as shown in Figure 1, electric shock pipe includes tube body (1), pipe lid (2), pin electrode array
(3) and printed circuit board (4).
Have the cavity (5) for accommodating target sample solution inside tube body (1), one end sealing of tube body (1), tube body (1) it is another
One end has the opening being connected with the cavity (5), and the edge of the opening has the end face of annular, and pipe lid (2) is using tool
There is the plastic material preparation of certain elasticity, pipe lid (2) internal diameter is less than or equal to the end face outer diameter of tube body (1) open edge annular.
One circle groove (6) of setting, fixed for pipe lid (2) on the outside of the end face of the annular, and phase is arranged on the inner face of the pipe lid (2)
Corresponding bulge-structure (7) is corresponding with groove (6) being arranged on the outside of the end face of annular.When pipe lid (2) is covered at tube body (1),
Micro-strain can occur for the inner face of pipe lid (2), have the end face gluing, sealing of annular with the edge of tube body (1) opening.
Multiple electrodes needle is welded on printed circuit board (4), is formed pin electrode array (3), and printed circuit board (4) is located at pipe
Cover at (2) external opening, pin electrode array (3) pass through pipe lid (2) it is internal, have at the top of printed circuit board (4) pad be electrically connected
Equipment is electrically connected.The shape of pin electrode array (3) is the regular hexagon being made of several equilateral triangles, described positive six
The outer ledge distance of side shape is consistent with tube body (1) internal diameter, pin electrode array (3) be inserted into tube body (1) after, outside pin electrode with
The fitting of tube body (1) inner wall, the pin electrode are located on the vertex of the equilateral triangle.The diameter of the electrode is 0.4 millimeter,
The distance between described two adjacent electrode central points are 1.1 millimeters, and the pin electrode is divided into three groups, and first group of electrode is positive
Pole, other two groups are cathode, are cathode around every anode electrode, are rotated by group polarity of electrode, Lai Shixian electrode district
Target solution in domain can receive the effect of electric field.
The utility model electric shock pipe, will in use, the mixed solution sample of cell and electrotransfection substance is injected into cavity
Pipe lid (2) is fixed on tube body (1) open end, and pin electrode array (3) touches tube body (1) bottom, and mixed solution sample uniformly divides
Cloth is internal in pin electrode array (3), is connect by the pad at the top of printed circuit board (4) with the pulse power, in pin electrode array
(3) internal to form electric field, micropore is generated in cell membrane surface using electroporation phenomenon, makes the target substance in mixed solution sample
Entered in cell by micropore.
Embodiment two
As shown in Fig. 2, the groove (6) being arranged on the outside of end face annular in the electric shock pipe structure of embodiment one, pipe lid (2)
Inner face on be arranged corresponding bulge-structure (7) using helicitic texture replace, specifically replace with the tube body (1) opening
Edge, which has, is arranged external screw thread (8) on the outside of the end face of annular, and internal screw thread (9) are arranged in the inner face of the pipe lid (2), pipe lid (2)
It can be fixedly connected by internal screw thread (9) with the external screw thread (8) on the tube body (1).
Embodiment three
On the basis of the electric shock pipe structure of embodiment one, grab is set, and pipe lid (2) setting can on tube body (1)
The buckle being mutually clamped with the grab.
Example IV
On the basis of the electric shock pipe structure of embodiment two, grab is set on pipe lid (2), energy is set on the tube body (1)
Enough buckles being mutually clamped with the grab.
Embodiment five
By the electric shock pipe in the utility model embodiment two, one is connected to up to electrotransfection instrument host, setting electricity turns condition
270 volts of voltage, 100 microsecond of pulse width, pulse number 6 times, the pulse spacing 1 second.
According to the description of the electric shock pipe of CN201410722470.9, the similar electric shock pipe of self-making structure is tested as a comparison,
It is connected to Celetrix electroporation host, HEK293F electricity is selected to turn condition.
Experimentation is as follows: HEK293F cell (human embryonic kidney cells) of the collection in logarithmic growth phase, and 1000 turns of revolving speed/
Minute, it is centrifuged 5 minutes, abandons supernatant.Cell is resuspended with electrotransfection buffer, so that the density of cell is 2 × 107A/milliliter, adds
Enter to need electrotransfection to be transferred to the plasmid pcDNA3.1-GFP of cell, make 20 mcg/ml of concentration of plasmid, gently piping and druming mixing
Uniformly.The bioblast electricity prepared is turned into buffer suspension and adds to the electric shock pipe neutralization of the embodiments of the present invention two respectively
In the electric shock pipe of CN201410722470.9, the processing of cell electrotransfection is carried out by cooperation electrotransfection instrument.
After electrotransfection, the cell suspension after transfection is placed in centrifuge tube, 1000 revs/min, is centrifuged 5 minutes.It abandons
Supernatant is added 293 FreeStyle culture mediums and cell, inoculation and triangular pyramidal culture in glassware, culture density 2 × 10 is resuspended6A/
Milliliter, is subsequently placed on shaking table and cultivates, and 125 revs/min of shaking speed, condition of culture: 37 degrees Celsius of temperature, gas concentration lwevel
5%.Electrotransfection efficiency is detected after 24 hours on cell flow type analyzer.
In the electric shock pipe provided by the observation to electrotransfection process, the utility model embodiment two, during electrotransfection
The bubble generated near electrode is less, and electrode surface does not change after electrotransfection.
By the way that electrotransfection Comparative result, according to the description of the electric shock pipe of CN201410722470.9, self-making structure is similar
The resulting cell electrotransfection efficiency of electric shock pipe in 67%-73% or so；And utilize the electric shock of the embodiments of the present invention two
Resulting cell electrotransfection efficiency is managed in 83%-85% or so.
Although the utility model has been described in detail, the modification in the spirit and scope of the utility model is for ability
Field technique personnel will be apparent.It should be understood that sheet described above and/or in the appended claims is practical
The part of novel aspect and various embodiments and various features can be combined or completely or partially be interchangeable.Such as this
Field technical staff will be realized that, in the description of aforementioned various embodiments, refers to those of another embodiment and implements
Scheme can suitably with other combination of embodiment.In addition, it will be recognized by those of ordinary skill in the art that foregoing description is only to make
To illustrate, and it is not intended to limit the utility model.
Claims (10)
1. a kind of electric shock pipe, including tube body (1), pipe lid (2) and pin electrode array (3), the tube body (1) is internal to be had for holding
Receive the cavity (5) of target liq sample, which is characterized in that one end of the tube body (1) seals, the other end of the tube body (1)
With the opening being connected with the cavity (5), the working position of the pin electrode array (3) is placed in the cavity (5), institute
The edge for stating opening has the end face of annular；The pin electrode array (3) is set in pipe lid (2), and can pass through the pipe
The external opening of lid (2) is electrically connected with outside, and the edge that the inner face of the pipe lid (2) can be open with tube body (1) has ring
The end face of shape is fixedly connected.
2. electric shock pipe according to claim 1, which is characterized in that printed circuit board is arranged in the external of the pipe lid (2)
(4)。
3. electric shock pipe according to claim 2, which is characterized in that the inner face and the tube body (1) of the pipe lid (2) are opened
The edge of mouth, which has, is respectively set screw thread on the outside of annular end face, and the tube body (1) is threadably secured with the pipe lid (2)
Connection.
4. electric shock pipe according to claim 2, which is characterized in that at least one circle of outside setting of the end face of the annular is recessed
Corresponding bulge-structure (7), the end face of the bulge-structure (7) and annular is arranged in slot (6), the inner face of the pipe lid (2)
Outside setting groove (6) it is corresponding, and form a fixed connection.
5. electric shock pipe according to claim 1-4, which is characterized in that grab is set on the tube body (1), it is described
The buckle that can be mutually clamped with the grab is set on pipe lid (2)；Or grab is set on the pipe lid (2), the tube body (1)
The buckle that upper setting can be mutually clamped with the grab.
6. electric shock pipe according to claim 5, which is characterized in that the pipe lid (2) and tube body (1) are connected by flexibility of linking rod
It connects.
7. electric shock pipe according to claim 3 or 4, which is characterized in that the inner face internal diameter of the pipe lid (2) is equal to described
The end face outer diameter of tube body (1) open edge annular；Or the inner face internal diameter of the pipe lid (2) is open less than the tube body (1)
The end face outer diameter of edge annular.
8. electric shock pipe according to claim 5, which is characterized in that the pin electrode array (3) includes multiple pin electrodes, institute
State in multiple pin electrodes that temporally the period applies the first polar electric pulse and the second polar electric pulse respectively on each electrode,
Wherein, what is be distributed around the corresponding electrode of the described first polar electric pulse is all the corresponding electricity of the second polar electric pulse
Pole, first polarity and the second polarity are opposite polarity.
9. electric shock pipe according to claim 8, which is characterized in that the pin electrode array (3) is in the cavity (5)
Length is identical as the length of the cavity (5).
10. electric shock according to claim 9 pipe, which is characterized in that the material of the pipe lid (2) is can deformation occurs has
Organic polymeric material, it is described can the organic polymer material that deformation occurs be plastics, rubber, plastic cement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821151341.9U CN208829688U (en) | 2018-07-20 | 2018-07-20 | A kind of electric shock pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821151341.9U CN208829688U (en) | 2018-07-20 | 2018-07-20 | A kind of electric shock pipe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208829688U true CN208829688U (en) | 2019-05-07 |
Family
ID=66306923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821151341.9U Active CN208829688U (en) | 2018-07-20 | 2018-07-20 | A kind of electric shock pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208829688U (en) |
-
2018
- 2018-07-20 CN CN201821151341.9U patent/CN208829688U/en active Active
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Address after: No.2 501, No.200, Shengpu Xingpu Road, Suzhou Industrial Park, Jiangsu Province Patentee after: SUZHOU ETTA BIOTECH CO., LTD. Address before: 215123 Ruoshui Road 388 E1504, Suzhou Industrial Park, Jiangsu Province Patentee before: SUZHOU ETTA BIOTECH CO., LTD. |