CN208829686U - A kind of electric shock pipe - Google Patents
A kind of electric shock pipe Download PDFInfo
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- CN208829686U CN208829686U CN201821150472.5U CN201821150472U CN208829686U CN 208829686 U CN208829686 U CN 208829686U CN 201821150472 U CN201821150472 U CN 201821150472U CN 208829686 U CN208829686 U CN 208829686U
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- pipe
- pipe lid
- electric shock
- tube body
- electrode
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Abstract
The utility model provides a kind of electric shock pipe, there is the cavity (6) for accommodating target liq sample including tube body (1), first electrode (4), second electrode (5), the first pipe lid (2) and the second pipe lid (3), inside the tube body (1).The electric shock pipe of the utility model, direction of an electric field is vertical with tube body, and effective electric field can be formed by filling it up with without liquid, carries out electrotransfection after liquid is filled it up in cavity, inside cavity not will form bubble and lead to electrode loose contact, can avoid electrode loose contact influence electricity and turns effect.
Description
Technical field
The utility model belongs to electrotransfection technical field, is related to a kind of electric shock pipe, is specifically related to a kind of plane electrode
Electric shock pipe.
Background technique
Since the 1970's electroporation technology just be used to for molecule importeding into zooblast or plant cell, researcher
It confirms, cell, which is exposed to, can make to form channel on after birth in of short duration lasting high voltage electric field, and macromolecular such as protein and DNA can lead to
The channel is crossed to enter into the cell.Electroporation be it is a kind of be widely used and highly recommended for test cell line and gene therapy
Method.When applying strong electrical field, cell membrane is temporarily changed to porous property and can be through foreign material, such as macromolecular.
Cell membrane electricity penetration depends on the various parameters of electric field, such as pulse pattern, pulse voltage, pulse duration, pulse
Quantity and other experiment conditions.
Equipment currently used for cell electroporation mainly has pole cup.
Describe a kind of electric shock pipe in the patent of US20130052711A1, effect be added in pipe containing cell and
Top electrode and lower electrode is respectively set in the upper/lower terminal of electric shock pipe, by thin in the liquid sample that the substance of cell will be injected
Born of the same parents' electroporation power supply is connected with top electrode and lower electrode, to make to form electric field in electric shock pipe, and then makes extracellular substance
Into intracellular.And after filling in liquid sample in electric shock pipe, before starting electric shock, need to prevent from having time in the pipe that shocks by electricity
Gas remains to form aeration electrical connection.In that patent, applicant has one and electric shock in design on the nozzle outer wall of electric shock pipe
The annular groove that tube chamber is connected, the sustainable injection liquid sample into electric shock tube chamber of experimenter is until liquid surface protrudes
After electric shock tube chamber, then it will power on and be contacted with liquid convex surface until being pressed onto the upper edge of electric shock tube chamber, formation is to cavity under polar cap
The sealing of the inside liquid, and the liquid overflowed on a small quantity flows in annular groove.This design can generally prevent from having in electric shock pipe
Residual air influences electrical connection.
But find in use, the electric shock pipe in above-mentioned patent is relatively high to the precise requirements of manufacture, while to reality
The requirement of the person's of testing operation is also relatively high.If upper inaccurate in manufacture, it is easy to cause the leakproofness on edge on electrode and cavity not
It is good, electrode may occur later under electrode cap and upwarp the feelings for leaving that edge causes outside air to reenter cavity on cavity again
Condition.In addition if operator's improper use when is also easy to cause in pipe and has residual air bubble, cell electroporation is influenced
Effect.Scientific experiment is very high to the reliability requirement of instrument and equipment, and therefore, above-mentioned electric shock pipe needs increase in manufacture
The design of reliability is improved, to improve the reliability of experimenter's operation.
Chinese patent CN201410722470 provides a kind of electric shock pipe and the cell electroporation with electric shock pipe, belongs to life
Object medicine instrument and equipment technical field.It solves existing cell electricity and turns the technical problems such as stability difference.The electric shock pipe includes pipe
Body, first electrode, second electrode and Guan Gai, tubular body have the cavity for accommodating target liq sample, one end of tube body
Equipped with first electrode, second electrode is set in pipe lid, and the outer end of second electrode can pass through the opening and external electrical of pipe lid
Couple, is provided with the elastic component being connected with second electrode in pipe lid, the outside of elastic component is connect with pipe lid, the inside of elastic component
It is connected with second electrode.The sealing performance between second electrode and opening can be effectively improved, to avoid that liquid is being added
Extraneous air enters in cavity when sample.
But at present there is following technical problem in the electric shock pipe when in use, first is that the treating capacity of electrotransfection is necessary every time
It greater than the capacity of cavity, causes to waste, cell liquid must overflow cavity, if cell liquid is less than the volume of cavity, will lead to the
Two electrodes do not reach liquid, influence electrotransfection effect；Second is that in electrotransfection, inside cavity formation bubble, and bubble nothing
Method excludes in time, and bubble adheres on the second electrode, influences electrotransfection effect.Third is that the electric shock pipe is when in use, it is necessary to use
High-voltage pulse processing, is easy to produce electric arc, influences operator's safety.
Summary of the invention
In order to solve the above-mentioned technical problem, it is approximate to provide a kind of surface structure for the utility model, but redesigns first
The electric shock pipe device of the position of electrode and second electrode.
The technical solution that the utility model uses is: a kind of electric shock is managed, including tube body, first electrode, second electrode,
First pipe lid and the second pipe lid, the tubular body have the cavity for accommodating target liq sample, which is characterized in that described
First electrode and the second electrode are set to the symmetric side of the inside cavity, and the both ends of the tube body have and the chamber
The opening that body is connected；The edge of the one or both ends opening of the tube body has annular end face, the inner end of the first pipe lid
Face can be fixedly connected with the annular end face at edge open at one end；The second pipe lid can be fixed with another end opening of tube body
Connection, the second pipe lid can be such that first electrode and second electrode is electrically connected respectively with outside formation by electric connecting sheet.
It is preferred that the inner face of the first pipe lid and the edge open at one end of the tube body have the outside point of annular end face
Not She Zhi screw thread, the inner face of the first pipe lid can have annular end face by the edge open at one end of screw thread and the tube body
Outside is fixedly connected.
It is preferred that at least one circle groove of outside setting of the annular end face, the inner face setting of the first pipe lid are corresponding convex
Structure is played, the bulge-structure is corresponding with the groove that the outside of annular end face is arranged.
It is preferred that grab is arranged on the tube body, the first pipe lid and/or the second pipe cover setting can be with the grab
The mutually buckle of clamping.Or the first pipe lid and/or the second pipe cover setting grab, on the tube body setting can with it is described
The buckle that grab is mutually clamped.
It is preferred that first pipe covers setting buckle, second pipe covers setting grab；Or first pipe covers
Grab is set, and second pipe covers setting buckle.
It is preferred that the first pipe lid and/or the second pipe lid are connect with tube body by flexibility of linking rod.
It is preferred that the inner face internal diameter of the first pipe lid is less than or equal to body open edge annular end face outer diameter.
It is preferred that the material of the first pipe lid and/or the second pipe lid be can the organic polymer material that deformation occurs, that is, have
Have a material of certain elasticity, it is described can the organic polymer material that deformation occurs be plastics, rubber, plastic cement etc..
The beneficial technical effect of the utility model
The electric shock pipe of the utility model, with the structure provided according to CN201410722470 compared with similar electric shock pipe, electricity
Field direction is vertical with tube body, filled it up with without liquid can be formed in effective electric field cavity fill it up with liquid after carry out electrotransfection, cavity
Inside not will form bubble and lead to electrode loose contact, can avoid because liquid is less than or bubble causes to lose between electrode and is electrically connected
It connects or is electrically connected poor contact influence electricity and turn effect.
Detailed description of the invention
Fig. 1 electric shock pipe overall structure diagram provided by the utility model；
Attached drawing annotation: 1, tube body, the 2, first pipe lid, the 3, second pipe lid, 4, first electrode, 5, second electrode, 6, cavity, 7,
Groove, 8, bulge-structure.
Specific embodiment
The preferred embodiment of the utility model is described in detail with reference to the accompanying drawing, so that the advantages of the utility model
It can be easier to be readily appreciated by one skilled in the art with feature, to make the protection scope of the utility model apparent clear
Define.
Embodiment one
Electric shock pipe provided by the utility model is as shown in Figure 1, electric shock pipe includes tube body (1), the first pipe lid (2), the second pipe
Cover (3), first electrode (4) and second electrode (5).
There is the cavity (6) for accommodating target sample solution inside tube body (1), the both ends of cavity (6) have and the cavity (6)
The edge of the opening being connected, the opening has annular end face, and first electrode (4) and the second electrode (5) are set to institute
The internal symmetric side of cavity (6) is stated, at least one circle groove (7) of setting, the inner end of the first pipe lid (2) on the outside of the annular end face
Corresponding bulge-structure (8) is arranged in face, corresponding with groove (7) being arranged on the outside of annular end face.First pipe lid (2) is using tool
There is the plastic material preparation of certain elasticity, the first pipe lid (2) internal diameter is less than or equal to outside tube body (1) open edge annular end face
Diameter.When the first pipe lid (2) is assembled to tube body (1), micro-strain can occur for the inner face of the first pipe lid (2), with tube body (1)
The annular end face sealing of open edge is bonded.
Second pipe lid (3) is the adapter tube lid that is electrically connected, and there is the electrode for being responsible for electrical connection in inside, when being assembled to tube body (1), with pipe
First electrode (4) and second electrode (5) connection in body (1) form electrical connection.Second pipe lid (3) outer diameter is less than or approaches
Tube body (1) internal diameter, when the second pipe lid (3) is fixed on tube body (1), the inner face of the second pipe lid (3) can be open with tube body (1)
The end face seal of inside edge is bonded.
Electric shock pipe forms cavity closed at one end in use, the second pipe lid (3) is first assembled to tube body (1), by cell and
The mixed solution sample of electrotransfection substance is injected into cavity, and the first pipe lid (2) is fixed on tube body (1) open end, passes through second
Pipe lid (3) is connect with the pulse power, is formed electric field inside first electrode (4) and second electrode (5), is existed using electroporation phenomenon
Cell membrane surface generates micropore, enters the target substance in mixed solution sample in cell by micropore.
Embodiment two
The groove (7) being arranged on the outside of annular end face in the electric shock pipe structure of embodiment one, the inner face of the first pipe lid (2)
The corresponding bulge-structure (8) of upper setting specifically replaces with the inner face of the first pipe lid (2) and described using helicitic texture replacement
The edge of tube body (1) opening, which has, is respectively set screw thread on the outside of annular end face, and the inner face of the first pipe lid (2) can pass through screw thread
Have with the edge of the tube body (1) opening and is fixedly connected on the outside of annular end face.
Embodiment three
On the basis of the electric shock pipe structure of embodiment one, grab, the first pipe lid (2) setting are set on tube body (1)
The buckle that can be mutually clamped with the grab.
Example IV
On the basis of the electric shock pipe structure of embodiment two, grab is set on the first pipe lid (2), is set on the tube body (1)
Set the buckle that can be mutually clamped with the grab.
Embodiment five
By the electric shock pipe in the utility model embodiment two, one is connected to up to electrotransfection instrument host, setting electricity turns condition
210 volts of voltage, 1 millisecond of pulse width, pulse number 6 times, the pulse spacing 1 second.
According to the description of the electric shock pipe of CN201410722470.9, the similar electric shock pipe of self-making structure is tested as a comparison,
It is connected to Celetrix electroporation host, HEK293F electricity is selected to turn condition.
Preparatory work of experiment process is as follows: collecting the HEK293F cell (human embryonic kidney cells) for being in logarithmic growth phase, revolving speed 1000
Rev/min, it is centrifuged 5 minutes, abandons supernatant.Cell is resuspended with electrotransfection buffer, so that the density of cell is 2 × 107A/milli
It rises, addition needs electrotransfection to be transferred to the plasmid pcDNA3.1-GFP of cell, makes 20 mcg/ml of concentration of plasmid, gently blows
It beats and is uniformly mixed.The bioblast electricity prepared is turned into buffer suspension and adds to the electric shock pipe of the embodiments of the present invention one respectively
In the electric shock pipe for neutralizing comparative experiments, the processing of cell electrotransfection is carried out by cooperation electrotransfection instrument.
After electrotransfection, the cell suspension after transfection is placed in centrifuge tube, 1000 revs/min, is centrifuged 5 minutes.It abandons
Supernatant is added 293 FreeStyle culture mediums and cell, inoculation and triangular pyramidal culture in glassware, culture density 2 × 10 is resuspended6A/
Milliliter, is subsequently placed on shaking table and cultivates, and 125 revs/min of shaking speed, condition of culture: 37 degrees Celsius of temperature, gas concentration lwevel
5%.Electrotransfection efficiency is detected after 24 hours on cell flow type analyzer.
Through the observation to electrotransfection process, in the electric shock pipe of the embodiments of the present invention one, during electrotransfection
The bubble generated near electrode is less, and electrode surface does not change after electrotransfection.
By the way that electrotransfection Comparative result, according to the description of the electric shock pipe of CN201410722470.9, self-making structure is similar
The resulting cell electrotransfection efficiency of electric shock pipe in 67%-73% or so；And the electric shock pipe institute of the embodiments of the present invention one
The cell electrotransfection efficiency obtained is in 88%-92% or so.
Although the utility model has been described in detail, the modification in the spirit and scope of the utility model is for ability
Field technique personnel will be apparent.It should be understood that sheet described above and/or in the appended claims is practical
The part of novel aspect and various embodiments and various features can be combined or completely or partially be interchangeable.Such as this
Field technical staff will be realized that, in the description of aforementioned various embodiments, refers to those of another embodiment and implements
Scheme can suitably with other combination of embodiment.In addition, it will be recognized by those of ordinary skill in the art that foregoing description is only to make
To illustrate, and it is not intended to limit the utility model.
Claims (10)
1. a kind of electric shock pipe, including tube body (1), first electrode (4), second electrode (5), the first pipe lid (2) and the second pipe lid
(3), there is the cavity (6) for accommodating target liq sample, which is characterized in that the first electrode inside the tube body (1)
(4) and the second electrode (5) is set to the internal symmetric side of the cavity (6), and the both ends of the tube body (1) have and institute
State the opening that cavity (6) is connected；The tube body (1) one or both ends opening edge have annular end face, described first
The inner face of pipe lid (2) can be fixedly connected with the annular end face at the edge open at one end of tube body (1)；The second pipe lid (3)
It can be fixedly connected with another end opening of tube body (1), the second pipe lid (3) can make first electrode by electric connecting sheet
(4) it is electrically connected respectively with outside formation with second electrode (5).
2. electric shock pipe according to claim 1, which is characterized in that the inner face and the tube body of the first pipe lid (2)
(1) edge open at one end, which has, is respectively set screw thread on the outside of annular end face, and the inner face of the first pipe lid (2) can pass through spiral shell
Line has with the edge open at one end of the tube body (1) to be fixedly connected on the outside of annular end face.
3. electric shock pipe according to claim 1, which is characterized in that at least one circle groove of outside setting of the annular end face
(7), corresponding bulge-structure (8) is arranged in the inner face of the first pipe lid (2), and the bulge-structure (8) is outer with annular end face
The groove (7) of side setting is corresponding.
4. electric shock pipe according to claim 1-3, which is characterized in that buckle is set on the tube body (1), it is described
Setting can be with the grab for buckling and being mutually clamped on first pipe lid (2) and/or the second pipe lid (3).
5. electric shock pipe according to claim 1-3, which is characterized in that the first pipe lid (2) and/or the second pipe
It covers and buckle is set on (3), setting can be with the grab for buckling and being mutually clamped on the tube body (1).
6. electric shock pipe according to claim 1-3, which is characterized in that buckle is set on the first pipe lid (2),
Grab is set on the second pipe lid (3)；Or grab is set on the first pipe lid (2), it is arranged on the second pipe lid (3)
Buckle.
7. electric shock according to claim 6 pipe, which is characterized in that the first pipe lid (2) and/or the second pipe lid (3) with
Tube body (1) is connected by flexibility of linking rod.
8. electric shock pipe according to claim 3, which is characterized in that the inner face internal diameter of the first pipe lid (2) is less than pipe
Body (1) open edge annular end face outer diameter.
9. electric shock pipe according to claim 2, which is characterized in that the inner face internal diameter of the first pipe lid (2) is equal to pipe
Body (1) open edge annular end face outer diameter.
10. electric shock pipe according to claim 8 or claim 9, which is characterized in that the first pipe lid (2) and/or the second pipe lid
(3) material be can the organic polymer material that deformation occurs, it is described can the organic polymer material that deformation occurs be plastics, rubber
Glue, plastic cement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821150472.5U CN208829686U (en) | 2018-07-20 | 2018-07-20 | A kind of electric shock pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821150472.5U CN208829686U (en) | 2018-07-20 | 2018-07-20 | A kind of electric shock pipe |
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| Publication Number | Publication Date |
|---|---|
| CN208829686U true CN208829686U (en) | 2019-05-07 |
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ID=66306833
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821150472.5U Active CN208829686U (en) | 2018-07-20 | 2018-07-20 | A kind of electric shock pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208829686U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112824530A (en) * | 2019-11-20 | 2021-05-21 | 中国科学院深圳先进技术研究院 | HEK293F suspension cell high-efficiency electrotransfection method |
-
2018
- 2018-07-20 CN CN201821150472.5U patent/CN208829686U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112824530A (en) * | 2019-11-20 | 2021-05-21 | 中国科学院深圳先进技术研究院 | HEK293F suspension cell high-efficiency electrotransfection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: No.2 501, No.200, Shengpu Xingpu Road, Suzhou Industrial Park, Jiangsu Province Patentee after: SUZHOU ETTA BIOTECH CO., LTD. Address before: 215123 Ruoshui Road 388 E1504, Suzhou Industrial Park, Jiangsu Province Patentee before: SUZHOU ETTA BIOTECH CO., LTD. |
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| CP02 | Change in the address of a patent holder |