CN114480536A - Preparation method and application of soybean, coffee and sealwort bioactive peptide - Google Patents
Preparation method and application of soybean, coffee and sealwort bioactive peptide Download PDFInfo
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- CN114480536A CN114480536A CN202111550122.4A CN202111550122A CN114480536A CN 114480536 A CN114480536 A CN 114480536A CN 202111550122 A CN202111550122 A CN 202111550122A CN 114480536 A CN114480536 A CN 114480536A
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- coffee
- soybean
- sealwort
- bioactive peptide
- peptide
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- 244000068988 Glycine max Species 0.000 title claims abstract description 55
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- 230000000975 bioactive effect Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 12
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- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
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- 230000000415 inactivating effect Effects 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 9
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- 108090000526 Papain Proteins 0.000 claims abstract description 5
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 5
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 5
- 239000004365 Protease Substances 0.000 claims abstract description 5
- 102000004142 Trypsin Human genes 0.000 claims abstract description 5
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- 239000012670 alkaline solution Substances 0.000 claims abstract description 5
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- 239000012588 trypsin Substances 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims abstract description 4
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- SQFSKOYWJBQGKQ-UHFFFAOYSA-N kaempferide Chemical compound C1=CC(OC)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 SQFSKOYWJBQGKQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 3
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- 240000007154 Coffea arabica Species 0.000 abstract description 36
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- 150000002333 glycines Chemical class 0.000 abstract 1
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 229940080701 chymosin Drugs 0.000 description 6
- 108010007119 flavourzyme Proteins 0.000 description 6
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- 239000000463 material Substances 0.000 description 5
- 108090000145 Bacillolysin Proteins 0.000 description 4
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- 108091005507 Neutral proteases Proteins 0.000 description 4
- 102000035092 Neutral proteases Human genes 0.000 description 4
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 description 4
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- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 2
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- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 244000148687 Glycosmis pentaphylla Species 0.000 description 1
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 1
- URLZCHNOLZSCCA-VABKMULXSA-N Leu-enkephalin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)CNC(=O)CNC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=CC=C1 URLZCHNOLZSCCA-VABKMULXSA-N 0.000 description 1
- 108010056079 Subtilisins Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
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- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 1
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- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
- 229940107187 fructooligosaccharide Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
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- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Immunology (AREA)
- Diabetes (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
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- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
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- Polymers & Plastics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
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- Hematology (AREA)
Abstract
A preparation method and application of soybean, coffee and rhizoma polygonati bioactive peptides are disclosed, wherein the raw materials comprise soybean, rhizoma polygonati and coffee, and the specific method comprises the following steps: mixing semen glycines 30-50 parts, rhizoma Polygonati 5-15 parts, and coffee 15-40 parts. Firstly, preparing a separated alkaline protein solution, separating soybean, polygonatum and coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, and inactivating enzymes after enzymolysis; blending with purified water according to the proportion of 1-5%, adding flavoring agent, plant preservative and purified water to obtain raw materials, and making into cosmetics, foods or medicines and health products. The invention can improve and improve the comprehensive immunity of users with less usage amount, has the effects of invigorating qi and nourishing yin, strengthening spleen, moistening lung and tonifying kidney, has certain curative effect on weakness of spleen and stomach, deficiency of kidney essence, internal heat and thirst, tiredness and hypodynamia, dry mouth and poor appetite, lung deficiency and dry cough, insufficient essence and blood and other symptoms, especially diabetes mellitus, and simultaneously has the effects of enriching blood and nourishing qi, resisting aging and reducing blood pressure.
Description
Technical Field
The invention relates to the field of bioactive peptides, in particular to a preparation method and application of a soybean, coffee and sealwort bioactive peptide.
Background
Bioactive Peptides (BAP) are a generic term for various Peptides ranging from dipeptides to complex linear, cyclic structures, composed of 20 natural amino acids in different compositions and arrangements in proteins, and are multifunctional compounds derived from proteins. The bioactive peptide has various human metabolism and physiological regulation functions, is easy to digest and absorb, has the effects of promoting immunity, regulating hormone, resisting bacteria and viruses, reducing blood pressure, reducing blood fat and the like, and is the hottest research topic and functional factor with great development prospect in the current international food industry.
The existing bioactive peptide types do not take soybeans, sealwort and coffee as raw materials, so that the bioactive peptide types are not rich enough, the nutritional ingredients are not diverse enough, and the requirements of consumers cannot be met.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a preparation method and application of a soybean, coffee and sealwort bioactive peptide.
In order to achieve the aim of the invention, the invention adopts the specific scheme that:
a preparation method of soybean, coffee and rhizoma polygonati bioactive peptide comprises the following steps of preparing raw materials of soybean, rhizoma polygonati and coffee: mixing 30-50 parts of soybean, 5-15 parts of rhizoma polygonati and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the rhizoma polygonati and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, carrying out enzymolysis, and then inactivating enzyme.
Further, the protein raw materials of the soybeans, the sealwort and the coffee are prepared by enzymolysis of pepsin and trypsin.
Further, the protein raw materials of the soybeans, the sealwort and the coffee are prepared by papain or compound enzyme Protamex enzymolysis.
Further, the mass ratio of the soybean to the sealwort to the kaempferide is (1.3-3.1): (1.3-2.2): (1.8-4.1).
Further, the mass ratio of the soybean, the polygonatum and the coffee compound peptide is (1.3-4.0): 0.42.
further, removing surface impurities from the composite micromolecular ionic peptide liquid, and centrifuging to obtain supernatant, namely the composite soybean polygonatum coffee micromolecular (ionic) peptide.
An application of bioactive peptide from soybean, coffee and sealwort in preparing cosmetics, food, medicine and health-care food is prepared from soybean, coffee and sealwort through proportional mixing with purified water (1-5%), adding flavouring, plant antiseptic and purified water, and preparing food, medicine and health-care food.
The invention has the beneficial effects that:
the compound plant small-molecule soybean coffee sealwort peptide liquid with specific molecular weight regions is prepared by a specific process, and three or four of the three or four compounds are compounded in a proper proportion, so that the compound plant small-molecule soybean coffee sealwort peptide liquid can improve the comprehensive immunity of a user with a small using amount, has the effects of tonifying qi and nourishing yin, tonifying spleen, moistening lung and tonifying kidney, has certain curative effects on diseases such as weakness of spleen and stomach, deficiency of kidney essence, internal heat and thirst, tiredness and hypodynamia, dry mouth and poor appetite, lung deficiency and dry cough, insufficiency of essence and blood and the like, particularly diabetes, and has the effects of enriching blood and nourishing qi, resisting aging and reducing blood pressure.
Detailed Description
The present invention is further described below by way of specific examples, but the present invention is not limited to only the following examples. Variations, combinations, or substitutions of the invention, which are within the scope of the invention or the spirit, scope of the invention, will be apparent to those of skill in the art and are within the scope of the invention.
A composite health-care process using the health-care function of peptide, coffee fragrance and health-care function of siberian solomonseal rhizome includes such steps as preparing the alkaline solution of protein, separating from soybean, coffee and siberian solomonseal rhizome by mixing (30-70 portions, 5-15 portions and 15-40 portions). Adding Alcalase protease into the alkaline solution obtained by separating soybean, coffee and rhizoma polygonati, carrying out enzymolysis, inactivating enzyme, and centrifuging to obtain supernatant, namely the composite soybean coffee rhizoma polygonati peptide solution. Carrying out reflux ultrafiltration on the soybean, rhizoma polygonati, caffeine and enkephalin liquid in a greenhouse, and carrying out adsorption deodorization on activated carbon at the same time of the ultrafiltration. And performing series connection on 9 ultrafiltration membranes in the ultrafiltration operation to intercept molecular weight of 2500 Da-4500 Da and pressure of 0.2 Mpa-1.2 Mpa, treating for 5-20 min, removing inorganic salt ions with relative molecular mass less than or equal to 2500Da, and collecting the soybean coffee sealwort composite peptide liquid. And adding flavoring agents such as orange and strawberry liquid, fructo-oligosaccharide, xylitol, fruit sugar and the like in a greenhouse to improve the taste of the finished product of the compound peptide liquid. The oral liquid with health care and treatment functions is produced.
Example 1
The raw materials comprise the following components in parts by weight: 80 parts of compound soybean, polygonatum sibiricum and coffee compound peptide, 2 parts of crystalline fructose and 2 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
s1, dissolving the composite soybean polygonatum coffee peptide in water (the mass ratio of materials to water is 1:10), adjusting the pH of the solution to 8.5 by using 1mol/L NaOH solution, adding alkaline protease for enzymolysis, then adjusting the pH to 7 by using 1mol/L HC1 solution, adding neutral protease for enzymolysis, adding 2500U/kg of the neutral protease for enzymolysis, inactivating the enzyme after the enzymolysis is finished, centrifuging, taking supernate, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 1000U to obtain filtrate with the molecular weight of less than 1000U, thereby obtaining the soybean polygonatum coffee composite oligopeptide concentrated solution.
S2, mixing soybean, polygonatum sibiricum and coffee oligopeptides, dissolving the soybean, polygonatum sibiricum and coffee oligopeptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 7 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzymes after the reaction is finished, centrifuging, taking supernate, dialyzing, precooling for 50min at the temperature of-10 ℃ for 50min to obtain composite peptides, adding crystalline fructose and food additives, and dissolving and mixing uniformly to obtain the plant peptide oral liquid.
Example 2
The raw materials comprise the following components in parts by weight: 75 parts of soybean, polygonatum sibiricum and coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
s1, dissolving soybean polygonatum coffee peptide in water (the mass ratio of materials to water is 1:10), adjusting the pH of the solution to 8.5 by using 1mol/L NaOH solution, adding alkaline protease for enzymolysis, then adjusting the pH to 7 by using 1mol/L HC1 solution, adding neutral protease for enzymolysis, adding 2500U/kg of the neutral protease for enzymolysis, inactivating enzyme after the enzymolysis is finished, centrifuging, taking supernate, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 1000U to obtain filtrate with the molecular weight of less than 1000U, thereby obtaining the soybean polygonatum coffee composite oligomeric liquid.
S2, mixing soybean, polygonatum sibiricum and coffee oligopeptides, dissolving the soybean, polygonatum sibiricum and coffee oligopeptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 6 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzymes after the reaction is finished, centrifuging, taking supernate to obtain a compound peptide concentrated solution, adding crystalline fructose and a food additive, and dissolving and mixing uniformly to obtain the plant peptide oral liquid.
Comparative example 1
The raw materials comprise the following components in parts by weight: 75 parts of soybean, polygonatum sibiricum and coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
adding crystalline fructose and food additive into soybean, rhizoma Polygonati and coffee compound peptide, dissolving and mixing to obtain plant peptide oral liquid.
Comparative example 2
The raw materials comprise the following components in parts by weight: 75 parts of soybean, polygonatum sibiricum and coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter-reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
mixing soybean polygonatum coffee peptides, dissolving the mixture in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 6 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzyme after the reaction is finished, centrifuging, taking supernate, adding crystalline fructose and food additives, and dissolving and mixing uniformly to prepare the vegetable peptide liquid oral liquid.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (7)
1. A preparation method of soybean, coffee and rhizoma polygonati bioactive peptide is characterized in that the raw materials comprise soybean, rhizoma polygonati and coffee, and the specific method comprises the following steps: mixing 30-50 parts of soybean, 5-15 parts of rhizoma polygonati and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the rhizoma polygonati and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, carrying out enzymolysis, and then inactivating enzyme.
2. The method for preparing the bioactive peptide from soybean, coffee and sealwort according to claim 1, wherein the bioactive peptide comprises the following components: the protein raw materials of the soybeans, the sealwort and the coffee are prepared by enzymolysis of pepsin and trypsin.
3. The method for preparing the bioactive peptide from soybean, coffee and sealwort according to claim 1, wherein the bioactive peptide comprises the following components: the protein raw materials of the soybeans, the sealwort and the coffee are prepared by papain or compound enzyme Protamex enzymolysis.
4. The method for preparing a bioactive peptide from soybean, coffee and sealwort as claimed in any one of claims 1 to 3, wherein: the mass ratio of the soybean to the sealwort to the kaempferide is (1.3-3.1): (1.3-2.2): (1.8-4.1).
5. The method for preparing bioactive peptide from soybean, coffee and sealwort according to any one of claims 1-3, wherein the bioactive peptide is prepared by the following steps: the mass ratio of the soybean, the sealwort and the coffee compound peptide is (1.3-4.0): 0.42.
6. the method for preparing a bioactive peptide from soybean, coffee and sealwort as claimed in claim 1, wherein the bioactive peptide comprises the following components: removing surface impurities from the composite micromolecular ionic peptide liquid, and centrifuging to obtain supernatant, namely the composite soybean polygonatum coffee micromolecular (ionic) peptide.
7. The application of the soybean, coffee and sealwort bioactive peptide is characterized in that: blending with purified water according to the proportion of 1-5%, adding flavoring agent, plant preservative and purified water to obtain raw materials, and making into cosmetics, foods or medicines and health products.
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Application publication date: 20220513 |