CN114190480A - Superoxide dismutase microcapsule and preparation method and application thereof - Google Patents
Superoxide dismutase microcapsule and preparation method and application thereof Download PDFInfo
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- 102000019197 Superoxide Dismutase Human genes 0.000 title claims abstract description 67
- 108010012715 Superoxide dismutase Proteins 0.000 title claims abstract description 67
- 239000003094 microcapsule Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 30
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 14
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229920001661 Chitosan Polymers 0.000 claims abstract description 13
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 13
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- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 10
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- 102000013142 Amylases Human genes 0.000 claims abstract description 7
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- 239000004365 Protease Substances 0.000 claims abstract description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 7
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- 239000011259 mixed solution Substances 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 19
- 108090000790 Enzymes Proteins 0.000 claims description 19
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- 229910052708 sodium Inorganic materials 0.000 claims description 19
- 239000011734 sodium Substances 0.000 claims description 19
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- 238000002156 mixing Methods 0.000 claims description 8
- 239000005662 Paraffin oil Substances 0.000 claims description 6
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims description 6
- 239000003995 emulsifying agent Substances 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 240000008415 Lactuca sativa Species 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
- 235000012045 salad Nutrition 0.000 claims description 3
- 235000012424 soybean oil Nutrition 0.000 claims description 3
- 239000003549 soybean oil Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 229920001353 Dextrin Polymers 0.000 claims description 2
- 239000004375 Dextrin Substances 0.000 claims description 2
- 235000019425 dextrin Nutrition 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims description 2
- 210000004211 gastric acid Anatomy 0.000 abstract description 6
- 238000004945 emulsification Methods 0.000 abstract description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 5
- 239000003674 animal food additive Substances 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 3
- 239000011248 coating agent Substances 0.000 abstract description 3
- 238000000576 coating method Methods 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
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- 102000057297 Pepsin A Human genes 0.000 abstract description 2
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- 235000010410 calcium alginate Nutrition 0.000 abstract description 2
- 239000000648 calcium alginate Substances 0.000 abstract description 2
- 229960002681 calcium alginate Drugs 0.000 abstract description 2
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 abstract description 2
- 210000004051 gastric juice Anatomy 0.000 abstract description 2
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- 230000035790 physiological processes and functions Effects 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 1
- 230000008961 swelling Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- 229940088598 enzyme Drugs 0.000 description 14
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 12
- 150000003254 radicals Chemical class 0.000 description 7
- 239000002253 acid Substances 0.000 description 6
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 3
- 238000006701 autoxidation reaction Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 230000032683 aging Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
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- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- -1 superoxide anion free radical Chemical class 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
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- 238000001035 drying Methods 0.000 description 1
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- 150000002632 lipids Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Inorganic Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention belongs to the technical field of feed additives, and particularly provides a superoxide dismutase microcapsule as well as a preparation method and application thereof. Different from the traditional method of coating by using chitosan after separating oil phase, the preparation method provided by the invention is that the chitosan is added into sodium alginate and calcium carbonate, the mixture is mixed with superoxide dismutase to form water phase after swelling in water, then the oil phase is added for emulsification, glacial acetic acid is added for stirring, and standing is carried out to separate the oil phase, so that the obtained calcium alginate gel bead is firmer than the traditional method, and the stability and the gastric acid resistance of the dried SOD microcapsule are better. When the SOD is mixed with protease, amylase and a carrier to be used as a feed additive, the probability that the SOD is decomposed by gastric acid and pepsin after the feed enters a gastric juice environment in a digestive tract can be reduced, so that the physiological function of the SOD in the intestinal tract can be fully exerted, the immunity and disease resistance of a fed animal can be enhanced, and the survival rate can be improved.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a superoxide dismutase microcapsule as well as a preparation method and application thereof.
Background
Superoxide dismutase (SOD) is a metal enzyme that can catalyze superoxide anion free radicals to produce disproportionation reactions. The superoxide anion free radical is used as a free radical generated in the metabolic process of organisms, can attack biological macromolecules, such as lipid, protein, nucleic acid, polyunsaturated fatty acid and the like, causes the damage of cell structures and functions, and has close relation with the aging and pathological changes of organisms. SOD can specifically remove superoxide anion free radicals in organisms, thereby resisting damage of the superoxide free radicals to biological macromolecules and organelles, eliminating damage of exogenous oxygen free radicals to the organisms and playing an important role in maintaining the balance of the oxygen free radicals of the organisms. As the most effective enzyme for eliminating superoxide radicals, SOD has the characteristics of radiation resistance, aging resistance, oxidation resistance and tumor prevention, is widely applied to the industries of cosmetics, medicines, health products and foods, and has great application potential and wide development prospect. In the feed industry, SOD is added to remove free radicals in animals, improve the oxidation resistance of intestinal tracts, improve the immunity and disease resistance, enhance the resistance and improve the survival rate.
At present, SOD mostly has poor acid resistance and poor stability, therefore, the invention microencapsulates SOD to protect SOD from being damaged in gastric acid, thereby normally playing the effect, and prepares a multifunctional feeding type enzyme preparation on the basis of the effect.
Disclosure of Invention
The invention aims to overcome the problems of acid resistance and poor stability of SOD in the prior art.
Therefore, the invention provides a preparation method of complex superoxide dismutase microcapsules, which comprises the following steps:
(1) weighing sodium alginate, chitosan and calcium carbonate, adding water to swell, and obtaining a sodium alginate-chitosan-calcium carbonate mixed solution;
(2) adding superoxide dismutase into the mixed solution of sodium alginate-chitosan-calcium carbonate, and mixing to obtain water phase;
(3) adding an emulsifier into the oil phase, adding the water phase into the oil phase, and stirring and emulsifying to obtain a W/O emulsion;
(4) adding glacial acetic acid into the W/O emulsion, stirring, standing, separating oil phase and water phase, and removing oil phase to obtain superoxide dismutase microcapsule.
Specifically, by mass concentration, the sodium alginate-chitosan-calcium carbonate mixed solution in the step (1) contains 1.5-2.5% of sodium alginate, 0.1-0.5% of chitosan, and the mass ratio of calcium carbonate to sodium alginate is 1: (2-4).
Specifically, the volume ratio of the superoxide dismutase to the sodium alginate-chitosan-calcium carbonate mixed solution in the step (2) is 1: (2-4).
Specifically, the oil phase in the step (3) includes one of paraffin oil, soybean oil or salad oil.
Specifically, the emulsifier in the step (3) comprises Span 80.
Specifically, the volume ratio of the water phase to the oil phase in the step (3) is 1: (2-5).
Specifically, the adding amount of the glacial acetic acid in the step (4) is 0.2-0.6% by volume ratio.
The invention also provides a feed type compound enzyme preparation, which comprises the superoxide dismutase microcapsule prepared by the method, protease, amylase and a carrier; wherein the enzyme activity of the superoxide dismutase in the superoxide dismutase microcapsule is 500-1000U/g; the enzyme activity of the protease is 3000-5000U/g; the enzyme activity of the amylase is 500-1000U/g.
Specifically, the carrier comprises soluble starch, dextrin and stone powder.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the preparation method of the superoxide dismutase microcapsule provided by the invention is different from the traditional method of coating by using chitosan after separating oil phase, but chitosan is added into sodium alginate and calcium carbonate to obtain sodium alginate-chitosan-calcium carbonate mixed solution, then the sodium alginate-chitosan-calcium carbonate mixed solution is mixed with SOD to form water phase, then oil phase is added for emulsification, glacial acetic acid is added for stirring, standing is carried out to separate oil phase, and the obtained calcium alginate gel bead embedded with chitosan and SOD is firmer than the traditional method, so that the SOD microcapsule obtained after drying has better stability and gastric acid resistance. When the SOD is mixed with protease, amylase and a carrier to be used as a feed additive, the probability that the SOD is decomposed by gastric acid and pepsin after the feed enters a gastric juice environment in a digestive tract can be reduced, so that the physiological function of the SOD in the intestinal tract can be fully exerted, the immunity and disease resistance of a fed animal can be enhanced, and the survival rate can be improved.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Although representative embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that various modifications and changes may be made thereto without departing from the scope of the invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be defined by the appended claims and equivalents thereof.
The invention provides a preparation method of superoxide dismutase microcapsules, which comprises the following steps:
(1) weighing 1.5-2.5% of sodium alginate, 0.1-0.5% of chitosan and calcium carbonate by mass concentration, wherein the mass ratio of the calcium carbonate to the sodium alginate is 1: (2-4) adding water to swell for 1-2h to obtain a sodium alginate-chitosan-calcium carbonate mixed solution;
(2) adding superoxide dismutase into the mixed solution of sodium alginate-chitosan-calcium carbonate, and mixing completely to obtain water phase; the volume ratio of the mixed solution of superoxide dismutase and sodium alginate-chitosan-calcium carbonate is 1: (2-4);
(3) adding 1-2% of emulsifier into the oil phase, adding the water phase into the oil phase, stirring at 400r/min for 15min, and emulsifying to obtain W/O emulsion;
wherein the oil phase comprises one of paraffin oil, soybean oil or salad oil; emulsifiers include Span 80; the volume ratio of the water phase to the oil phase is 1: (2-5);
(4) adding 0.2-0.6% of glacial acetic acid into the W/O emulsion according to the volume ratio, continuously stirring for 1h, standing for 2-4h at 4 ℃, separating an oil phase and a water phase by using a separating funnel, and removing an oil phase to obtain the superoxide dismutase microcapsule.
The effect of the superoxide dismutase microcapsules of the present invention is examined below by way of specific examples.
Example 1:
the embodiment provides a superoxide dismutase microcapsule, which is prepared by the following steps:
(1) weighing 2% of sodium alginate, 0.5% of chitosan and 0.5% of calcium carbonate by mass concentration, adding water to swell for 2 hours to obtain a sodium alginate-chitosan-calcium carbonate mixed solution;
(2) adding 10ml of SOD enzyme solution obtained by yeast fermentation into 20ml of sodium alginate-chitosan-calcium carbonate mixed solution, and fully and uniformly mixing to obtain a water phase for later use;
(3) adding 2% Span80 as oil phase into paraffin oil, adding water phase into 70ml oil phase, stirring at 400r/min for 15min for emulsification to obtain W/O emulsion;
(4) and adding 0.6ml of glacial acetic acid into the W/O emulsion, continuously stirring for 1h, standing for 4h at 4 ℃, separating an oil phase and a water phase by using a separating funnel, and removing an oil phase to obtain the SOD microcapsule.
The enzymatic activity of the SOD microcapsule at 25 ℃ is 3500U/ml by a pyrogallol autoxidation method; the enzyme activity retention rate reaches 100 percent when the product is stored for 6 months at 25 ℃; the product is stored for 2 weeks at the high temperature of 45 ℃, and the retention rate reaches more than 90 percent.
The pyrogallol autoxidation method comprises the following specific steps:
preparing solution A and solution B, wherein the solution A is 0.1mol/L Tris-HCl buffer solution (containing 1mmol/L EDTA-2Na) with pH of 8.2; the solution B is 4.5mmol/L pyrogallol hydrochloride solution.
Definition of enzyme activity: the amount of SOD required to inhibit the 50% autoxidation rate of pyrogallol at 25 ℃ is one unit of activity.
Setting a blank group and an experimental group in a water bath kettle at 25 ℃, wherein the blank group comprises: sequentially adding 2.35mL of solution A, 2mL of distilled water and 0.15mL of solution B into a 10mL colorimetric tube; experimental groups: sequentially adding 2.35mL of solution A, 1.8mL of distilled water, 0.2mL of enzyme solution and 0.15mL of solution B into a 10mL colorimetric tube; immediately shaking and mixing after adding the solution B, pouring the mixed solution into a cuvette, and respectively measuring the initial and 1min after-light absorption values of the blank group and the experimental group under the condition of 325nm wavelength, wherein the difference is the self-oxidation rate of the pyrogallol. And finally, calculating the activity of the sample by adjusting the concentration of the enzyme solution to enable the auto-oxidation rate of the pyrogallol in the experimental group to be half of the auto-oxidation rate of the blank group.
The SOD microcapsules were treated at pH2.5 for 40 minutes, and the acid-resistant retention was found to be 75%.
Comparative example 1:
the embodiment provides a superoxide dismutase microcapsule, which is prepared by the following steps:
(1) weighing 2% of sodium alginate and 0.5% of calcium carbonate by mass concentration, adding water to swell for 2 hours to obtain a sodium alginate-calcium carbonate mixed solution;
(2) adding 10ml of SOD enzyme solution obtained by yeast fermentation into 20ml of sodium alginate-calcium carbonate mixed solution, and fully and uniformly mixing to obtain a water phase for later use;
(3) adding 2% Span80 as oil phase into paraffin oil, adding water phase into 70ml oil phase, stirring at 400r/min for 15min for emulsification to obtain W/O emulsion;
(4) adding 0.6ml of glacial acetic acid into the W/O emulsion, continuously stirring for 1h, standing at 4 ℃ for 4h, separating an oil phase and a water phase by using a separating funnel, removing an oil phase, and coating with a 0.5% chitosan solution for 30min to obtain the SOD microcapsule.
The acid-resistant storage rate of the SOD microcapsules after 40 minutes of treatment at pH2.5 was 68% as measured in the same manner as in example 1.
Comparative example 2:
the acid-resistant storage rate of the unencapsulated SOD enzyme solution after 40 minutes of treatment at pH2.5 was measured to be 13% in the same manner as in example 1.
From the above results of example 1 and comparative example 1, it is clear that the SOD microcapsules prepared by the method of the present invention have improved gastric acid resistance compared to SOD microcapsules prepared by the conventional method. From the results of example 1 and comparative example 2, it is clear that the acid resistance of the SOD microcapsules prepared by the method of the present invention is much higher than that of untreated SOD enzyme solution, and the enzyme activity of the original SOD enzyme solution is not affected by the microencapsulation.
Example 2:
the embodiment provides a feeding complex enzyme preparation, which is prepared by the following steps:
(1) weighing 2% of sodium alginate, 0.3% of chitosan and 1% of calcium carbonate by mass concentration, adding water to swell for 2 hours, and obtaining a sodium alginate-chitosan-calcium carbonate mixed solution;
(2) adding 10ml of SOD enzyme solution obtained by yeast fermentation into 20ml of sodium alginate-chitosan-calcium carbonate mixed solution, and fully and uniformly mixing to obtain a water phase for later use;
(3) adding 2% Span80 as oil phase into paraffin oil, adding water phase into 70ml oil phase, stirring at 400r/min for 15min for emulsification to obtain W/O emulsion;
(4) and adding 0.6ml of glacial acetic acid into the W/O emulsion, continuously stirring for 1h, standing for 4h at 4 ℃, separating an oil phase and a water phase by using a separating funnel, and removing an oil phase to obtain the SOD microcapsule.
(5) Mixing 500U/g SOD microcapsule with 1000U/g amylase and 5000U/g protease to obtain soluble starch, and spray drying to obtain the feed type complex enzyme preparation.
The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.
Claims (10)
1. A preparation method of superoxide dismutase microcapsules is characterized by comprising the following steps:
(1) weighing sodium alginate, chitosan and calcium carbonate, adding water to swell, and obtaining a sodium alginate-chitosan-calcium carbonate mixed solution;
(2) adding superoxide dismutase into the mixed solution of sodium alginate-chitosan-calcium carbonate, and mixing to obtain water phase;
(3) adding an emulsifier into the oil phase, adding the water phase into the oil phase, and stirring and emulsifying to obtain a W/O emulsion;
(4) adding glacial acetic acid into the W/O emulsion, stirring, standing, separating oil phase and water phase, and removing oil phase to obtain superoxide dismutase microcapsule.
2. The method for preparing superoxide dismutase microcapsules as claimed in claim 1, wherein: according to mass concentration, the sodium alginate-chitosan-calcium carbonate mixed solution in the step (1) contains 1.5-2.5% of sodium alginate, 0.1-0.5% of chitosan, and the mass ratio of calcium carbonate to sodium alginate is 1: (2-4).
3. The method for preparing superoxide dismutase microcapsules as claimed in claim 1, wherein: the volume ratio of the superoxide dismutase to the sodium alginate-chitosan-calcium carbonate mixed solution in the step (2) is 1: (2-4).
4. The method for preparing superoxide dismutase microcapsules as claimed in claim 1, wherein: the oil phase in the step (3) comprises one of paraffin oil, soybean oil or salad oil.
5. The method for preparing superoxide dismutase microcapsules as claimed in claim 1, wherein: the emulsifier in the step (3) comprises Span 80.
6. The method for preparing superoxide dismutase microcapsules as claimed in claim 1, wherein: the volume ratio of the water phase to the oil phase in the step (3) is 1: (2-5).
7. The method for preparing superoxide dismutase microcapsules as claimed in claim 1, wherein: and (3) the adding amount of the glacial acetic acid in the step (4) is 0.2-0.6% by volume ratio.
8. A superoxide dismutase microcapsule characterized in that: the superoxide dismutase microcapsules are prepared by the method according to any one of claims 1 to 7.
9. A feed type complex enzyme preparation is characterized by comprising the following components: the superoxide dismutase microcapsules, protease, amylase, and carrier of claim 8; wherein the enzyme activity of the superoxide dismutase in the superoxide dismutase microcapsule is 500-1000U/g; the enzyme activity of the protease is 3000-5000U/g; the enzyme activity of the amylase is 500-1000U/g.
10. A feed type complex enzyme preparation is characterized in that: the carrier comprises soluble starch, dextrin and stone powder.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005168A (en) * | 2012-12-27 | 2013-04-03 | 上海海洋大学 | Microbial lysozyme microcapsule as well as preparation and application of microbial lysozyme microcapsule |
| CN109601811A (en) * | 2019-01-10 | 2019-04-12 | 江苏德禧生物科技有限公司 | A kind of selenium-rich bifid bacterium microcapsule |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005168A (en) * | 2012-12-27 | 2013-04-03 | 上海海洋大学 | Microbial lysozyme microcapsule as well as preparation and application of microbial lysozyme microcapsule |
| CN109601811A (en) * | 2019-01-10 | 2019-04-12 | 江苏德禧生物科技有限公司 | A kind of selenium-rich bifid bacterium microcapsule |
Non-Patent Citations (1)
| Title |
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| 周淑青: "超氧化物歧化酶复合微球的制备及其活性考察", 浙江大学学报(医学版), pages 666 - 670 * |
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