CN113106011A - Array type constant temperature control device for detecting new corona RNA viruses - Google Patents
Array type constant temperature control device for detecting new corona RNA viruses Download PDFInfo
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- CN113106011A CN113106011A CN202110335418.8A CN202110335418A CN113106011A CN 113106011 A CN113106011 A CN 113106011A CN 202110335418 A CN202110335418 A CN 202110335418A CN 113106011 A CN113106011 A CN 113106011A
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- 241001493065 dsRNA viruses Species 0.000 title claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 39
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 241000711573 Coronaviridae Species 0.000 claims abstract description 10
- 241000700605 Viruses Species 0.000 claims abstract description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000005259 measurement Methods 0.000 claims abstract description 6
- 239000002184 metal Substances 0.000 claims abstract description 3
- 239000000741 silica gel Substances 0.000 claims abstract description 3
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 4
- 239000000523 sample Substances 0.000 abstract description 7
- 238000009396 hybridization Methods 0.000 abstract description 3
- 108020003215 DNA Probes Proteins 0.000 abstract description 2
- 239000003298 DNA probe Substances 0.000 abstract description 2
- 238000005057 refrigeration Methods 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- 238000000429 assembly Methods 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses an array type constant temperature control device for detecting new corona RNA viruses, which comprises a small measuring cell and a constant temperature control mechanism; the constant temperature control mechanism consists of a thermistor, a heating and refrigerating sheet, a radiator and a control circuit, wherein the upper surface of the heating and refrigerating sheet is attached to a metal part at the bottom of the measuring pool, the bottom surface of the heating and refrigerating sheet is connected with the radiator through heat-conducting silica gel, and the thermistor is arranged on the small measuring pool; the constant temperature control mechanism is respectively connected with the thermistor, the heating and refrigerating sheet and the radiator, and the small measuring cell and the constant temperature control mechanism form an array structure for multi-channel measurement of RNA virus samples. The constant temperature control device can be used for simultaneously measuring multiple samples of the new coronavirus RNA virus, heating, refrigerating and insulating the solution of the measured sample, and can accurately control the measurement temperature to be within the range of 15-80 ℃, so that a DNA probe and the RNA molecule of the measured virus have good hybridization efficiency, and the precision of the measurement device is ensured.
Description
Technical Field
The invention relates to a constant temperature control device, in particular to an array type constant temperature control device for detecting new corona RNA viruses.
Background
When the piezoelectric DNA sensor is used for detecting virus RNA nucleic acid molecules, the DNA primers and the RNA molecules only have high-efficiency hybridization efficiency within a certain temperature range, and experiments prove that the piezoelectric DNA sensor has high response at 25-37 ℃, and the temperature has large influence on the frequency stability of a quartz wafer in the piezoelectric sensor, so that the piezoelectric DNA sensor and virus sample liquid need to be subjected to constant temperature control. In the prior art, high-precision temperature resolution and strong control capability cannot be achieved, and the measurement precision and the frequency signal stability of the piezoelectric DNA sensor are seriously influenced. The invention detects the nucleic acid molecules of the new coronavirus RNA virus, so that the temperature of the detected virus sample solution is kept constant, and a higher electrochemical reaction rate can be kept.
Disclosure of Invention
The invention provides an array type constant temperature control device for detecting a novel coronavirus RNA virus, which aims to solve the technical problem of unstable temperature control of a biological sample in the prior art.
The invention provides an array type constant temperature control device for detecting new corona RNA viruses, which comprises: a small measuring cell and a constant temperature control mechanism; the constant temperature control mechanism includes: the device comprises a thermistor, a heating and refrigerating sheet, a radiator and a control circuit, wherein the thermistor, the heating and refrigerating sheet and the radiator form a stacked structure from top to bottom; the control circuit is respectively connected with the thermistor and the heating and refrigerating sheet through a DuPont wire, and the constant temperature control mechanism can control the heating and refrigerating sheet to refrigerate, heat or preserve heat within a certain temperature range.
The upper surface of the heating and refrigerating sheet is attached to a metal part at the bottom of the measuring pool, heat is conducted to the whole measuring pool, and meanwhile, heat is transferred to the sample liquid to be measured through the measuring pool; the lower surface of the heating and refrigerating piece is connected with the radiator through heat-conducting silica gel.
The small measuring cells are respectively provided with an independent constant temperature control mechanism, and the plurality of groups of measuring cells and the constant temperature control mechanisms form an array structure which is fixed in a groove of the measuring device and used for multi-channel parallel measurement of the measured virus sample.
The length of the heating and refrigerating piece is 2 cm-4 cm, the width of the heating and refrigerating piece is 2 cm-4 cm, and the power of the heating and refrigerating piece is 54 w-65 w.
The control circuit includes: the device comprises a controller unit, a keyboard input unit, a temperature acquisition unit, a heating and refrigerating sheet and a display unit; the temperature acquisition unit is respectively connected with the thermistor and the controller unit and is used for converting analog information acquired by the thermistor into digital information which can be identified by the controller unit; the heating and refrigerating piece is respectively connected with the controller unit and the heating and refrigerating piece, and the heating and refrigerating piece controls the heating and refrigerating piece to heat or refrigerate based on the control signal of the controller unit; the keyboard input unit is connected with the controller unit and is used for inputting temperature parameters; the display unit is connected with the controller unit and is used for displaying the current temperature of the sample liquid and the input temperature parameters; the controller unit sends out a control signal based on the data sent by the temperature acquisition unit.
The control temperature range is 15-80 ℃, and the error range of temperature acquisition is +/-0.5 ℃.
The invention has the beneficial effects that:
1. the error range of the constant temperature control device is +/-0.5 ℃, and the scheme has the beneficial effects of improving the temperature control precision and improving the frequency stability of the piezoelectric quartz wafer.
2. The temperature control range of the constant temperature control mechanism is between 15 ℃ and 80 ℃, so that the hybridization efficiency of the DNA probe and the virus RNA molecule to be detected can be improved, and the detection sensitivity of the piezoelectric sensor is enhanced.
3. The control circuit has extremely low power consumption and is very suitable to be used as a main controller of a biological sample liquid constant temperature control system.
Drawings
The features and advantages of the present invention will be more clearly understood by reference to the accompanying drawings, which are illustrative and not to be construed as limiting the invention in any way, and in which:
FIG. 1 is a schematic structural diagram of an array type constant temperature control device for detecting a novel coronavirus of the present invention;
FIG. 2 is a schematic diagram of the array measuring cell and the thermostatic control mechanism according to the present invention;
FIG. 3 is a schematic diagram of an array-type groove structure according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, an embodiment of the present invention provides an array type thermostatic control device for detecting a new coronavirus, including: a small measuring cell 1 and a constant temperature control mechanism 2;
the constant temperature control mechanism 2 includes: the device comprises a thermistor 3, a heating and refrigerating sheet 4, a radiator 5 and a control circuit 6;
constant temperature control mechanism 2 is stacked structure, down is thermistor 3, small-size measuring cell 1, heating refrigeration piece 4, thermal-insulated cotton 7, radiator 5 from last in proper order, and thermistor 3 installs on the pool wall of measuring cell 1, scribbles heat conduction cream 8 between thermal-insulated cotton 7 and the heating refrigeration piece 4, and the direct laminating of upper surface of heating refrigeration piece 4 is in the bottom of measuring cell 1, and the lower surface of heating refrigeration piece 4 passes through heat conduction cream 8 and is connected with radiator 5.
The temperature control range of the constant temperature control mechanism 2 is between 15 ℃ and 80 ℃, the larger temperature control range can meet the temperature requirement of biochemical reaction, the activity of the biological molecules is kept, and the optimal efficiency of the combination of the biological probe and the target virus molecule is achieved through temperature optimization and adjustment.
The error range of the constant temperature control mechanism 2 is +/-0.5 ℃, so that the stability of the oscillation frequency of a quartz wafer in the piezoelectric sensor can be ensured, and the detection precision of the piezoelectric sensor is improved.
As shown in fig. 2, the control circuit 6 is connected with the thermistor 3 and the heating and cooling chip 4 through a dupont line.
As shown in figure 3, the small measuring cell 1 and the constant temperature control mechanism 2 are completely attached to form an integral assembly, a plurality of assemblies can form an array assembly and are fixed in a bottom groove 9 of the device, the horizontal interval of adjacent grooves is 0.5 cm-1.5 cm, the longitudinal interval is 2.5cm, the array structure can be used for synchronous work of a plurality of constant temperature devices, a plurality of virus samples can be measured simultaneously, and the detection efficiency is improved.
The heating power of the heating and refrigerating sheet 4 can be between 54w and 65w, the power can meet the temperature control requirement of a small measuring cell, and the temperature can be increased by 1 ℃ within 1 minute.
One specific implementation and working method of the invention is as follows:
firstly, virus sample liquid is introduced into a small-sized measuring tank 1, the temperature of chemical reaction is set on a constant temperature control mechanism 2, a control circuit 6 receives a set temperature value and controls a heating and refrigerating sheet 4 to generate heat, a thermistor 3 detects the temperature of the measuring tank in real time, the heating and refrigerating sheet 4 stops heating once reaching the set temperature and keeps the temperature constant, if the temperature exceeds the set temperature, the heating and refrigerating sheet 4 is controlled to refrigerate, the control circuit adjusts the whole device to be within a set temperature deviation range, heat emitted from the bottom surface of the heating and refrigerating sheet 4 is conducted to a radiator 5 through a heat conducting paste 8 and heat insulation cotton 7, the assembly is prevented from being burnt due to overhigh temperature, a plurality of constant temperature control assemblies work simultaneously, and the requirement of simultaneous.
Claims (6)
1. The utility model provides a new coronary class RNA virus detection's array thermostatic control device, includes small-size measuring cell and thermostatic control mechanism, its characterized in that: the constant temperature control mechanism is of a stacking structure consisting of a thermistor, a heating and refrigerating sheet and a radiator from top to bottom, and the thermistor is fixed in the small measuring cell and used for collecting the temperature of sample liquid; the control circuit is respectively connected with the thermistor and the heating and refrigerating piece through DuPont wires, and the constant temperature control mechanism can control the heating and refrigerating piece to refrigerate, heat or preserve heat within a certain temperature range.
2. The array type thermostatic control device for detecting the new coronavirus RNA of claim 1, wherein: the upper surface of the heating and refrigerating sheet is attached to a metal part at the bottom of the measuring pool, heat is conducted to the whole measuring pool, and meanwhile, heat is transferred to the sample liquid to be measured through the measuring pool; the lower surface of the heating and refrigerating piece is connected with the radiator through heat-conducting silica gel.
3. The array type thermostatic control device for detecting the new coronavirus RNA of claim 1, wherein: the small measuring cells are respectively provided with an independent constant temperature control mechanism, and the plurality of groups of measuring cells and the constant temperature control mechanisms form an array structure for multi-channel parallel measurement of the tested virus sample.
4. The array type thermostatic control device for detecting the new coronavirus RNA of claim 1, wherein: the control temperature range is 15-80 ℃.
5. The array type thermostatic control device for detecting the new coronavirus RNA of claim 2, wherein: the heating and refrigerating sheet is 2 cm-4 cm long and 2 cm-4 cm wide.
6. The array type thermostatic control device for detecting the new coronavirus RNA of claim 2, wherein: the power of the heating and refrigerating piece is 54 w-65 w.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110335418.8A CN113106011A (en) | 2021-03-29 | 2021-03-29 | Array type constant temperature control device for detecting new corona RNA viruses |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110335418.8A CN113106011A (en) | 2021-03-29 | 2021-03-29 | Array type constant temperature control device for detecting new corona RNA viruses |
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| CN113106011A true CN113106011A (en) | 2021-07-13 |
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| CN202110335418.8A Pending CN113106011A (en) | 2021-03-29 | 2021-03-29 | Array type constant temperature control device for detecting new corona RNA viruses |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235196A (en) * | 2022-02-23 | 2022-03-25 | 常州先趋医疗科技有限公司 | Temperature detection device and method in LAMP amplification |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002214192A (en) * | 2001-01-19 | 2002-07-31 | Seiko Instruments Inc | Gene detection system, gene detecting device and method equipped therewith, and gene detecting chip |
| WO2003083537A1 (en) * | 2002-04-02 | 2003-10-09 | Sumiden Opcom,Ltd. | Temperature controller and array waveguide lattice type optical wavelength multiplexer/demultiplexer |
| US20050079393A1 (en) * | 2003-10-14 | 2005-04-14 | Hsi-Ming Shu | Method and system for controlling constant temperature for fuel cells |
| CN200970527Y (en) * | 2006-11-13 | 2007-11-07 | 方针 | Biochemical intelligent temperature control tester |
| CN101255396A (en) * | 2007-02-27 | 2008-09-03 | 索尼株式会社 | Nucleic acid amplifier |
| CN102071136A (en) * | 2009-11-25 | 2011-05-25 | 中国科学院电子学研究所 | Array micro polymerase chain reaction chip |
| CN206232730U (en) * | 2016-11-15 | 2017-06-09 | 杭州凯基科技有限公司 | The module thermal balance device of PCR gene amplification instrument |
| CN206337272U (en) * | 2016-07-07 | 2017-07-18 | 内蒙古医科大学附属医院 | A kind of Virus culture vessel |
| WO2018206122A1 (en) * | 2017-05-12 | 2018-11-15 | Universiteit Maastricht | Devices and methods for detecting viral particles |
| CN108841718A (en) * | 2018-08-31 | 2018-11-20 | 江苏天通设备科技有限公司 | A kind of bio-incubator active moisturizing heat patching device and method |
| CN110964633A (en) * | 2019-11-08 | 2020-04-07 | 江苏科技大学 | Constant temperature control system of biological sample liquid |
| CN111889160A (en) * | 2012-07-31 | 2020-11-06 | 简·探针公司 | Systems, methods, and apparatus for automated incubation |
-
2021
- 2021-03-29 CN CN202110335418.8A patent/CN113106011A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002214192A (en) * | 2001-01-19 | 2002-07-31 | Seiko Instruments Inc | Gene detection system, gene detecting device and method equipped therewith, and gene detecting chip |
| WO2003083537A1 (en) * | 2002-04-02 | 2003-10-09 | Sumiden Opcom,Ltd. | Temperature controller and array waveguide lattice type optical wavelength multiplexer/demultiplexer |
| US20050079393A1 (en) * | 2003-10-14 | 2005-04-14 | Hsi-Ming Shu | Method and system for controlling constant temperature for fuel cells |
| CN200970527Y (en) * | 2006-11-13 | 2007-11-07 | 方针 | Biochemical intelligent temperature control tester |
| CN101255396A (en) * | 2007-02-27 | 2008-09-03 | 索尼株式会社 | Nucleic acid amplifier |
| CN102071136A (en) * | 2009-11-25 | 2011-05-25 | 中国科学院电子学研究所 | Array micro polymerase chain reaction chip |
| CN111889160A (en) * | 2012-07-31 | 2020-11-06 | 简·探针公司 | Systems, methods, and apparatus for automated incubation |
| CN206337272U (en) * | 2016-07-07 | 2017-07-18 | 内蒙古医科大学附属医院 | A kind of Virus culture vessel |
| CN206232730U (en) * | 2016-11-15 | 2017-06-09 | 杭州凯基科技有限公司 | The module thermal balance device of PCR gene amplification instrument |
| WO2018206122A1 (en) * | 2017-05-12 | 2018-11-15 | Universiteit Maastricht | Devices and methods for detecting viral particles |
| CN108841718A (en) * | 2018-08-31 | 2018-11-20 | 江苏天通设备科技有限公司 | A kind of bio-incubator active moisturizing heat patching device and method |
| CN110964633A (en) * | 2019-11-08 | 2020-04-07 | 江苏科技大学 | Constant temperature control system of biological sample liquid |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235196A (en) * | 2022-02-23 | 2022-03-25 | 常州先趋医疗科技有限公司 | Temperature detection device and method in LAMP amplification |
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Application publication date: 20210713 |