CN112345505B - Method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene and application - Google Patents
Method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene and application Download PDFInfo
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- CN112345505B CN112345505B CN202011217629.3A CN202011217629A CN112345505B CN 112345505 B CN112345505 B CN 112345505B CN 202011217629 A CN202011217629 A CN 202011217629A CN 112345505 B CN112345505 B CN 112345505B
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Abstract
A method for detecting hypochlorite by tetra (4-aminobiphenyl) ethylene and an application thereof belong to the technical field of hypochlorite detection and analysis, the method comprises the steps of dissolving a tetra (4-aminobiphenyl) ethylene compound in an organic solvent to prepare a mother solution, and adding tetrahydrofuran and deionized water into the mother solution to prepare a fluorescent probe for detecting hypochlorite ions. Taking a plurality of groups of mother liquor in the process of preparing the fluorescent probe, respectively adding tetrahydrofuran and deionized water in different proportions into the plurality of groups of mother liquor to prepare a mixed solution, testing the fluorescence intensity of the mixed solution on a fluorescence analyzer, and taking a group with the maximum fluorescence intensity as the fluorescent probe for detecting hypochlorite ions. The fluorescent probe prepared by the method has higher accuracy in detecting hypochlorite ions.
Description
Technical Field
The invention relates to a method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene, belonging to the technical field of hypochlorite detection and analysis.
Background
Hypochlorous acid (HOCl), an important active oxygen, is generally generated from hydrogen peroxide and chloride ions under myeloperoxidase catalysis, and is generally spontaneously hydrolyzed in a neutral solution at pH 7.0 to form free hypochlorite ions. On the one hand, hypochlorite ions have important physiological roles in the living body, participate in numerous physiological activities, and are closely related to the immune system. On the other hand, research shows that excessive hypochlorite can cause tissue damage and diseases, such as neurodegenerative cancers, cardiovascular diseases, arthritis and other diseases, so that the development of a method for quickly, sensitively and specifically detecting hypochlorite ions has important research significance in the fields of environmental science, bioscience, food health and the like. The fluorescence chemical sensor has been a research focus in the fields of chemistry, environment, materials and the like because of a series of advantages of low cost, simple operation, real-time monitoring, high sensitivity, high selectivity and the like, and gradually advances to practicality.
Disclosure of Invention
The invention provides a rapid and cheap preparation method of a hypochlorite fluorescent probe, aiming at solving the problems in the prior art.
In order to achieve the purpose, the technical scheme provided by the invention is as follows: a method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene,
dissolving a tetra (4-aminobiphenyl) ethylene compound in an organic solvent to prepare a mother solution, and adding tetrahydrofuran and deionized water into the mother solution to prepare a fluorescent probe for detecting hypochlorite ions; hypochlorite ions were detected using a fluorescent probe.
The technical scheme is further designed as follows: taking a plurality of groups of mother liquor, respectively adding tetrahydrofuran and deionized water in different proportions into the plurality of groups of mother liquor to prepare a mixed solution, testing the fluorescence intensity of the mixed solution on a fluorescence analyzer, and taking a group with the maximum fluorescence intensity as a fluorescence probe for detecting hypochlorite ions.
The organic solvent is tetrahydrofuran.
One preferable scheme of the above technical scheme is as follows: tetrahydrofuran and deionized water were added to the mother liquor in a ratio of 1:4.
The concentration of the fluorescent probe for detecting hypochlorite ions is 40 mu M.
The application of the method for detecting hypochlorite by using the tetra (4-aminobiphenyl) ethylene in hypochlorite ion detection.
The invention has the beneficial effects that:
according to the hypochlorite detection method, the tetra (4-aminobiphenyl) ethylene compound is added with tetrahydrofuran and deionized water in a certain proportion to serve as a fluorescent probe, obvious fluorescence emission is achieved in a yellow-green light region (480-560 nm), regular linear quenching is achieved in hypochlorite solution with a certain concentration gradient through the fluorescence, the linear correlation coefficient R is 0.98, and the detection of hypochlorite ions by the tetra (4-aminobiphenyl) ethylene compound is high in accuracy. The method provides reliable technical support for quickly and accurately detecting hypochlorite ions.
Drawings
FIG. 1 is the AIE fluorescence curve of tetra (4-aminobiphenyl) ethylene compound in the example of the present invention;
FIG. 2 is a hypochlorite fluorescence quenching curve tested in the examples of the present invention;
FIG. 3 is a hypochlorite detection working curve tested in the examples of the present invention;
FIG. 4 is a graph showing the analysis of the effect of the addition of other active molecules and hypochlorite ions on the fluorescence intensity of a fluorescent probe for detecting hypochlorite ions in an example of the present invention;
FIG. 5 shows a structural formula of a tetra (4-aminobiphenyl) ethylene compound.
Detailed Description
The invention is described in detail below with reference to the figures and the specific embodiments.
Examples
The method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene in the embodiment comprises the following specific steps:
tetra (4-aminobiphenyl) ethylene compound AIE test. The specific structural formula of the tetra (4-aminobiphenyl) ethylene compound adopted in this example is shown in fig. 5. Dissolving the tetra (4-aminobiphenyl) ethylene compound in Tetrahydrofuran (THF) to prepare a 400 mu M solution serving as a mother solution, taking 10 glass bottles of 10ml, numbering 0-9 respectively, adding 1ml of the mother solution into each bottle, adding deionized water according to the proportion of 0, 10 percent and 20 percent … percent, and finally adding tetrahydrofuran to prepare 10ml of mixed solution. The fluorescence intensity of each group of mixed solution was measured on a fluorescence analyzer, as shown in fig. 1, which is the AIE fluorescence curve of the mixed solution prepared from the tetrakis (4-aminobiphenyl) ethylene compound, wherein the fluorescence intensity is the maximum when the deionized water content reaches 80%, the mixed solution of the ratio is selected as a fluorescence probe for detecting hypochlorite ions, and the probe concentration is 40 μ M.
And (3) drawing a working curve of the hypochlorite by using the fluorescent probe. A hypochlorite solution with a concentration of 5mM is prepared, 2.5ml of a fluorescent probe with a deionized water content of 80% is added into a clean cuvette, 0 to 25eq of the hypochlorite solution is gradually added into the cuvette, and a fluorescence titration spectrum in a tetrahydrofuran solution is tested as shown in FIG. 2. With the addition of hypochlorite, the fluorescence emission intensity of the solution at 482 nm decreases linearly, wherein the excitation wavelength is 317 nm. The fitting is carried out on the fluorescence intensity of each component at the position of 482 nm, the effect is good, and the linear correlation coefficient R is 0.98. As shown in FIG. 3, the work curve of detecting hypochlorite for the above-mentioned tetra (4-aminobiphenyl) ethylene compound was obtained.
And (4-aminobiphenyl) ethylene compound selectivity experimental test. Adding a solution to be detected, a 1000 mu M analyte and an analyte ClO into the mixed solution of the fluorescent probe for detecting hypochlorite ions - 、SCN - 、CO 3 2- 、S 2- 、NO 2 - 、H 2 O 2 、F - And then, performing fluorescence test, wherein the test result is shown in figure 4, which shows that the prepared fluorescence probe has high selectivity for detecting hypochlorite ions.
The technical solutions of the present invention are not limited to the above embodiments, and all technical solutions obtained by using equivalent substitution modes fall within the scope of the present invention.
Claims (2)
1. A method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene, which is characterized by comprising the following steps:
dissolving a tetra (4-aminobiphenyl) ethylene compound in tetrahydrofuran to prepare mother liquor, and adding tetrahydrofuran and deionized water into the mother liquor to prepare a mixed solution with the deionized water content of 80 percent as a fluorescent probe for detecting hypochlorite ions; hypochlorite ions were detected using a fluorescent probe.
2. Use of the method for detecting hypochlorite by using tetra (4-aminobiphenyl) ethylene as claimed in claim 1 in hypochlorite ion detection.
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