CN110122487B - Application of alcohol in attracting diaphorina citri and diaphorina citri attractant - Google Patents

Application of alcohol in attracting diaphorina citri and diaphorina citri attractant Download PDF

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CN110122487B
CN110122487B CN201910435422.4A CN201910435422A CN110122487B CN 110122487 B CN110122487 B CN 110122487B CN 201910435422 A CN201910435422 A CN 201910435422A CN 110122487 B CN110122487 B CN 110122487B
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diaphorina citri
attracting
alcohol
trans
cyclohexanediol
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CN110122487A (en
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彭婷
刘新军
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Gannan Normal University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/14—Catching by adhesive surfaces
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M2200/00—Kind of animal
    • A01M2200/01—Insects
    • A01M2200/012—Flying insects

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides application of alcohol in attracting diaphorina citri and a diaphorina citri attractant, and belongs to the technical field of plant disease and insect pest control. The invention attracts the diaphorina citri by using n-octanol or trans-1, 2-cyclohexanediol, and has high attracting efficiency. The invention can realize high-efficiency attraction to the diaphorina citri without adding other attraction substances, is simple, convenient and high-efficiency and is easy to implement. The data of the examples show that the attracting efficiency of n-octanol to diaphorina citri is as high as 49.28%, and the attracting efficiency of trans-1, 2-cyclohexanediol to diaphorina citri is as high as 47.50%.

Description

Application of alcohol in attracting diaphorina citri and diaphorina citri attractant
Technical Field
The invention relates to the technical field of plant disease and pest control, in particular to application of alcohol in attracting diaphorina citri and a diaphorina citri attractant.
Background
Phytophagous insects feed by identifying the characteristic odors of secondary metabolites contained in the host plant, which are the signal molecules that the insect seeks out of the host plant. Diaphorina citri (diaphorin itri Kuwym) is the major transmission vector for the pathogen of huanglongbing. The active application of the pesticide can control diaphorina citri, but can also cause various problems of drug resistance, environmental pollution, pesticide residue, natural enemies harm and the like. Therefore, many environmentally friendly methods for controlling diaphorina citri have been developed, such as essential oils, plant secondary metabolites, parasitic bees and entomopathogenic fungi, and yellow armyworm plates. However, these methods have a problem of low attracting efficiency.
Disclosure of Invention
In view of the above, the present invention aims to provide the use of an alcohol for attracting diaphorina citri and an attractant for diaphorina citri. The method uses alcohol to lure diaphorina citri, and the luring efficiency is high.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an application of alcohol in attracting diaphorina citri, wherein the alcohol is n-octanol or trans-1, 2-cyclohexanediol.
Preferably, the n-octanol is used in the form of a solution comprising n-octanol and mineral oil.
Preferably, the volume concentration of the n-octanol in the solution is 0.1-10%.
Preferably, the trans-1, 2-cyclohexanediol is used in the form of an aqueous solution.
Preferably, the volume concentration of the trans-1, 2-cyclohexanediol in the aqueous solution is 0.1-10%.
Preferably, the attracting time is more than or equal to 10 min.
Preferably, the application comprises the steps of:
and (3) coating the n-octanol on the armyworm yellow board, and then luring the diaphorina citri.
The invention also provides a diaphorina citri attractant which comprises alcohol and a solvent, wherein when the alcohol is n-octanol, the solvent is mineral oil, and when the alcohol is trans-1, 2-cyclohexanediol, the solvent is water.
Preferably, the volume concentration of the alcohol in the diaphorina citri attractant is 0.1-10%.
The invention provides application of alcohol in attracting diaphorina citri, wherein the alcohol is n-octanol or trans-1, 2-cyclohexanediol. The invention attracts the diaphorina citri by using n-octanol or trans-1, 2-cyclohexanediol, and has high attracting efficiency. The invention can realize high-efficiency attraction to the diaphorina citri without adding other attraction substances, is simple, convenient and high-efficiency and is easy to implement. The data of the examples show that the attracting efficiency of n-octanol to diaphorina citri is as high as 49.28%, and the attracting efficiency of trans-1, 2-cyclohexanediol to diaphorina citri is as high as 47.50%.
Detailed Description
The invention provides application of alcohol in attracting diaphorina citri, wherein the alcohol is n-octanol or trans-1, 2-cyclohexanediol.
In the present invention, the n-octanol is preferably used in the form of a solution, which preferably comprises n-octanol and mineral oil.
In the invention, the volume concentration of n-octanol in the solution is preferably 0.1-10%, and more preferably 1%.
In the present invention, the trans-1, 2-cyclohexanediol is preferably used in the form of an aqueous solution.
In the invention, the volume concentration of the trans-1, 2-cyclohexanediol in the aqueous solution is preferably 0.1-10%, and more preferably 10%.
In the invention, the attracting time is preferably not less than 10min, more preferably 10-60 min, most preferably 20-50 min, and further preferably 30-40 min.
In the present invention, the attraction is preferably performed at the evening.
The type of the mineral oil is not particularly limited in the present invention, and commercially available products known to those skilled in the art may be used.
In the present invention, the diaphorina citri is preferably an adult.
In the present invention, the application preferably comprises the steps of:
and (3) coating the alcohol on a pest sticking yellow board, and then luring the diaphorina citri.
The invention is not particularly limited with respect to the specific manner in which the coating may be obtained, and may be practiced using protocols well known to those skilled in the art. In the present invention, the diaphorina citri is attracted to the yellow sticky board, and the operation of attracting the diaphorina citri to the yellow sticky board is not particularly limited, and can be performed in a manner known to those skilled in the art.
In another aspect of the invention, the application preferably comprises the steps of:
shading the container containing the diaphorina citri;
the opening of the container is connected with a flavor source bottle filled with n-octanol through a rubber tube;
and taking the opening of the container as a boundary, and determining that the diaphorina citri is induced if the diaphorina citri crosses the boundary.
The specific manner of the light-shielding treatment in the present invention is not particularly limited, and a manner known to those skilled in the art may be used.
The present invention is not particularly limited in terms of the size and material of the container.
The diameter and the length of the rubber tube are not specially limited, and n-octanol can be volatilized into the container.
In the present invention, the taste source bottle preferably further comprises mineral oil.
The invention also provides a diaphorina citri attractant which comprises alcohol and a solvent, wherein when the alcohol is n-octanol, the solvent is mineral oil, and when the alcohol is trans-1, 2-cyclohexanediol, the solvent is water.
In the invention, the volume concentration of the alcohol in the diaphorina citri attractant is preferably 0.1-10%.
The use of n-octanol in attracting diaphorina citri and an attractant for diaphorina citri provided by the present invention are described in detail below with reference to the examples, which should not be construed as limiting the scope of the invention.
Example 1
Respectively setting solutions of n-octanol with volume concentrations of 0%, 0.1%, 1% and 10%, wherein 0% is blank control (namely mineral oil), connecting odor source bottles filled with volatile compounds with different concentrations at different bottle necks of the four-neck flask by rubber tubes with consistent lengths, respectively, placing 40 adults at the bottom of the four-neck flask at one time, taking a single-neck bottle passing through the four-neck flask as a boundary, regarding the boundary as induced, recording the quantity of diaphorina citri at each bottle neck and the total quantity of diaphorina citri at four bottle necks every 10 minutes, and calculating the induction efficiency. The experiment was repeated three times with the fresh psyllid replaced for each experiment. The statistics of the results are shown in table 1, and it can be seen from table 1 that the 1% n-octanol solution has better attractant for diaphorina citri.
TABLE 1 attraction efficiency (%), of solutions of n-octanol at different volume concentrations on diaphorina citri
Figure BDA0002070368940000031
Figure BDA0002070368940000041
Example 2
Comparing the attracting effect of the solution of n-octanol with the volume concentration of 1% and the mineral oil on the diaphorina citri by using a six-arm olfactometer, respectively placing the solution of n-octanol with the volume concentration of 1% and the mineral oil on two walls in the six-arm olfactometer, abandoning the other four arms for use, and sealing the arm openings. Before the experiment, the airflow flow rate of each arm of the six-arm olfactometer is set to be 80 mL/min. Each experiment tests 10 adults, and 30 adults are tested in total, and the test is carried out one by one. And (3) observing and recording the crawling track of the test insects after the test insects are placed in a smell instrument, recording the number of times that the diaphorina citri enters the initial test selection area as the number of times that a substance corresponding to the area enters the initial test selection area, and recording the number of times that the diaphorina citri stays for 3 minutes in the final selection area as the final selection number of times that the substance corresponding to the area is selected. As shown in table 2, it can be seen from table 2 that the method provided by the present invention has excellent attraction efficiency.
TABLE 2 comparison of the selection times and the final residence times of the diaphorina citri pairs
Substance(s) Number of entries Number of final dwells
Mineral oil 2 2
1% n-octanol solution 6 3
Example 3
(1) Connecting a taste source bottle filled with trans-1, 2-cyclohexanediol solutions with different concentrations with rubber tubes with the length of 30cm at different bottle necks of the four-neck flask respectively; preparing a trans-1, 2-cyclohexanediol solution by using ultrapure water, wherein the volume concentration is 0%, 0.1%, 1% and 10%, and 0% is a blank control;
(2) shading the four-neck flask containing the psyllids, putting about 40 adults at the bottom of the flask at one time, regarding a single-neck bottle passing through the four-neck flask as a boundary, regarding the psyllids passing through the boundary as being induced, recording the quantity of the citrus psyllids at each bottle mouth and the total quantity of the citrus psyllids at four bottle mouths every 10 minutes, and calculating the induction efficiency. The experiment was repeated three times with the fresh psyllid replaced for each experiment.
The results are shown in Table 3. According to the table 3, the trans-1, 2-cyclohexanediol has obvious induction effect on the diaphorina citri within the concentration range of 0.1-10%, the trans-1, 2-cyclohexanediol with the concentration of 10% has better induction effect on the diaphorina citri, and the induction rate can still reach about 47.50% when the induction time is 60 min.
TABLE 3 Induction efficiency (%) of various concentrations of trans-1, 2-cyclohexanediol against diaphorina citri
Figure BDA0002070368940000051
Example 4
(1) Connecting areas 1 and 2 of the six-arm olfactometer with the odor source bottle, and leaving the rest four areas vacant, wherein area 2 is set as a control area;
(2) opening a vacuum pump, adjusting the airflow speed of each arm of the six-arm olfactometer to 80mL/min, and waiting for the airflow to be stable;
(3) 1% trans-1, 2-cyclohexanediol solution (solvent is ultrapure water) is put into the source bottle corresponding to the area 1, and the same amount of ultrapure water is put into the source bottle corresponding to the area 2 as a control.
Each experimental test was conducted head by head, and the times of the psyllids entering different areas and the times of the areas where the psyllids remained for 3 minutes were recorded as the test results of the tropism reaction of the diaphorina citri to different volatile compounds, and the test results are shown in table 4. As can be seen from Table 4, trans-1, 2-cyclohexanediol had a good attractant effect against diaphorina citri.
TABLE 4 comparison of the selection times and the final residence times of diaphorismate for trans-1, 2-cyclohexanediol
Volatile matter Number of entries Number of final dwells
Ultrapure water 1 1
1% trans-1, 2-cyclohexanediol 8 7
Example 5
In an insect cage with the specification of 75 x 90(cm), insect trapping experiments are carried out by combining yellow plates (with the specification of 15 x 20cm), and the coating amount of the medicine on each yellow plate is about 1mL (the medicine can be increased by a proper amount). Coating 1% of n-octanol on a yellow plate, symmetrically suspending the yellow plate and the drug-free yellow plate in an insect cage, putting a murraya paniculata in the middle of the insect cage, putting about 30 psyllids in the insect cage, and luring the psyllids, wherein the experimental results are shown in table 5, and the experimental results prove that 1% of n-octanol has a luring effect on the psyllids.
In an insect cage with the specification of 75 x 90(cm), insect trapping experiments are carried out by combining yellow plates (with the specification of 15 x 20cm), and the coating amount of the medicine on each yellow plate is about 1mL (the medicine can be increased by a proper amount). The method comprises the steps of coating 1% trans-1, 2-cyclohexanediol in volume concentration on a yellow board, symmetrically suspending the yellow board and the yellow board without a medicine in an insect cage, putting a murraya paniculata in the middle of the insect cage, putting about 30 psyllids in the insect cage, supplementing about 1mL of the medicine on the yellow board on the next day, and attracting the psyllids, wherein the experimental results are shown in table 5, and the experimental results prove that the 1% trans-1, 2-cyclohexanediol has an attraction effect on the psyllids.
TABLE 51% n-octanol and 10% trans-1, 2-cyclohexenol trap results
Item Day one The next day The third day
Control 3 4 4
N-octyl alcohol 8 10 13
Trans-1, 2-cyclohexanediols 4 8 10
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (5)

1. Use of an alcohol, said alcohol being trans-1, 2-cyclohexanediol, for attracting diaphorina citri.
2. Use according to claim 1, characterized in that the trans-1, 2-cyclohexanediols are used in the form of an aqueous solution.
3. The use according to claim 2, wherein the volume concentration of trans-1, 2-cyclohexanediol in the aqueous solution is 0.1 to 10%.
4. The use as claimed in claim 1, wherein the attracting time is 10min or more.
5. The application according to claim 1, characterized in that it comprises the following steps:
and (3) coating the alcohol on a pest sticking yellow board, and then luring the diaphorina citri.
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CN103141459A (en) * 2013-03-26 2013-06-12 华中农业大学 Compound-type diaphorina citri trap with combination of smell volatile matter and color, and method
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