CN109527238A - A kind of preparation method of the piglet feed of enteric solubility compound micro-ecological preparation - Google Patents
A kind of preparation method of the piglet feed of enteric solubility compound micro-ecological preparation Download PDFInfo
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- CN109527238A CN109527238A CN201811480203.XA CN201811480203A CN109527238A CN 109527238 A CN109527238 A CN 109527238A CN 201811480203 A CN201811480203 A CN 201811480203A CN 109527238 A CN109527238 A CN 109527238A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 150000001875 compounds Chemical class 0.000 title claims abstract description 30
- 239000003094 microcapsule Substances 0.000 claims abstract description 39
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 239000000463 material Substances 0.000 claims abstract description 21
- 239000011162 core material Substances 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 239000002131 composite material Substances 0.000 claims abstract description 10
- 239000006041 probiotic Substances 0.000 claims abstract description 10
- 235000018291 probiotics Nutrition 0.000 claims abstract description 10
- 108010068370 Glutens Proteins 0.000 claims abstract description 9
- 235000021312 gluten Nutrition 0.000 claims abstract description 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 8
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 6
- 241000186673 Lactobacillus delbrueckii Species 0.000 claims abstract description 6
- 239000000661 sodium alginate Substances 0.000 claims abstract description 6
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 6
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 4
- 241000191996 Pediococcus pentosaceus Species 0.000 claims abstract description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 4
- -1 sodium alginate propylene glycol ester Chemical class 0.000 claims abstract description 4
- 239000008188 pellet Substances 0.000 claims abstract description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims abstract 6
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000004945 emulsification Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 2
- 239000008158 vegetable oil Substances 0.000 claims description 2
- 241000186660 Lactobacillus Species 0.000 claims 1
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- 235000019198 oils Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
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- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 230000003053 immunization Effects 0.000 abstract 1
- 244000005706 microflora Species 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 238000000034 method Methods 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- HDSBZMRLPLPFLQ-UHFFFAOYSA-N Propylene glycol alginate Chemical compound OC1C(O)C(OC)OC(C(O)=O)C1OC1C(O)C(O)C(C)C(C(=O)OCC(C)O)O1 HDSBZMRLPLPFLQ-UHFFFAOYSA-N 0.000 description 4
- 239000002775 capsule Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000770 propane-1,2-diol alginate Substances 0.000 description 4
- 235000010409 propane-1,2-diol alginate Nutrition 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 2
- 239000005862 Whey Substances 0.000 description 2
- 102000007544 Whey Proteins Human genes 0.000 description 2
- 108010046377 Whey Proteins Proteins 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- MWKXCSMICWVRGW-UHFFFAOYSA-N calcium;phosphane Chemical compound P.[Ca] MWKXCSMICWVRGW-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
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- 239000003995 emulsifying agent Substances 0.000 description 2
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- 239000004519 grease Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
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- 239000000203 mixture Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000707825 Argyrosomus regius Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 241001071864 Lethrinus laticaudis Species 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-L Malonate Chemical compound [O-]C(=O)CC([O-])=O OFOBLEOULBTSOW-UHFFFAOYSA-L 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 206010034960 Photophobia Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 208000013469 light sensitivity Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000008542 thermal sensitivity Effects 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/427—Pentosaceus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of preparation methods of the piglet feed of enteric solubility compound micro-ecological preparation.The preparation method of the piglet feed includes: that compound micro-ecological preparation of the invention is that mixing fermentation culture is carried out by strains such as Bifidobacterium, lactobacillus bulgaricus, Lactobacillus delbrueckii, Pediococcus pentosaceus as core material, using sodium alginate propylene glycol ester and Gluten range of hydrolysed peptides as wall material, mixing probiotics are embedded, a kind of feeding microcapsules are made.Compound micro-ecological preparation prepared by the invention can be used as many animals feed addictive, is directly fed after mixing evenly or commercial pellet feed is made with conventional feed processing technology.Composite bacteria is not only increased to the tolerance of poor environment, so that its safety is spent the influence of processing conditions and stomach acidic environment to strain activity, and can discharge rapidly in enteron aisle, being proliferated composite bacteria rapidly is dominant microflora, plays bigger effect.Without any hormonal components in such feed, has the function of enhancing animal immunizing power, can effectively enhance the disease resistance of sucking pig.
Description
Technical field
The invention belongs to field of fodder, and in particular to a kind of piglet feed preparation method of compound micro-ecological preparation.
Background technique
Antibiotic once played a significant role in field of fodder, because the diffusion of its bring drug resistance, medicament residue and ecology are broken
The negative effect such as bad is increasingly severe, and people gradually begin look for new substitute.With environmental consciousness and Consciousness of food security
Reinforcement, there is an urgent need to novel green feed addictives to improve feed product quality.Micro ecological preparation such as lactic acid bacteria is in recent years
To pay attention to extensively because of it, because itself is resided in animal body, adjusts animal alimentary canal microbial flora, strengthen immunity is one
Plant safe and efficient microorganism formulation.
But it because lactic acid bacteria resistance is poor, is easily inactivated under bad condition, loses original physiological activity, seriously affect in fact
With value.For example, reaching stomach acidic environment after many microorganism formulations are taken and being difficult to survive, only minute quantity microorganism
Enteron aisle can be reached, therefore probiotic effects are very little.
Microencapsulation is a kind of method that the substances such as solid, liquid, gas body are embedded in small closed capsule.This method is not only
Can effective protection and isolated substance, reduce oxidation reaction, be passivated light sensitivity and thermal sensitivity, moreover it is possible to effectively shielding core material
Bad smell controls the rate of release of core material, changes the physicochemical property (such as dispersibility, color, shape, density) of core material, improves
Core material storage stability.
Sucking pig refers to just birth to the piggy for growing to 30 kilograms, they grow lacks rapidly innate immunity again.Therefore right
Great care is needed in the nursing of sucking pig, otherwise sucking pig easily ill or growth retardation, cause the rate of animals delivered to the slaughter-house to decline, and cause economic loss.
In order to solve problem above, it is often solved by increasing the dosage of probiotics in feed processing and livestock and poultry breeding industry
Certainly, but this method cannot not only tackle the problem at its root, and at high cost.Therefore research and development embedding techniques, to probiotics into
The method of row embedding is come into being.In Chinese patent CN1276009A, discloses a kind of pair of lactic acid bacteria and carry out secondary coating
Technology improves lactic acid bacteria to the tolerance of gastric acid, but because it cannot be discharged rapidly in enteron aisle, not can be effectively controlled cause of disease
Bacterium, lactic acid bacteria can not play maximum effectiveness.It is current micro- glue using sodium alginate as wall material in Chinese patent CN1569043
Common wall material in capsule coating, but sodium alginate moisture holding capacity itself is poor, and microcapsules dehydration, which is easily hardened, to be crushed, therefore cannot be effective
Probiotics is protected, it is made to play maximum effectiveness.
Existing various methods exist mostly is unable to effective protection beneficial flora, and cost is excessively high or probiotic microcapsule obtained
The drawbacks such as spawn activity is low are unsuitable for being widely applied in feed industry and the livestock and poultry cultivation production of meagre profit.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation sides of the piglet feed of microcapsules for adding compound micro-ecological preparation
Method can effectively achieve the purpose that enteric, can discharge rapidly in enteron aisle, make probiotics with occupying dominant bacteria rapidly
Position plays maximum effectiveness.And composite bacteria is used, single culture is compared, probiotic effects are more significant.
The present invention to achieve the above object, using following technical scheme:
A kind of piglet feed of compound micro-ecological preparation, the compound micro-ecological preparation are Bifidobacterium, bulgarian milk bar
The strains such as bacterium, Lactobacillus delbrueckii, Pediococcus pentosaceus carry out mixing fermentation culture and are made.
The preparation method of the microcapsules of the compound micro-ecological preparation includes the preparation of composite fermentation liquid, wall material aqueous solution
Preparation, gluey microcapsules preparation and vacuum freeze-drying finished product microcapsules.
The core material of the microcapsules is complex microorganism bacterium solution；Wall material is propylene glycol alginate and Gluten range of hydrolysed peptides；
The core material of the microcapsules and the ratio of wall material are as follows: 0.05-0.15:1.
The preparation of the composite fermentation liquid is inoculated with various lactic acid bacterias into fluid nutrient medium, in 30-40 DEG C of shaking table culture
12-24 hours, revolving speed 100-150 r/min obtained seed liquor；Seed liquor is inoculated into one by the inoculum concentration of inoculum concentration 5-15%
In grade seed culture medium, in mutually synthermal culture 6-10 hours, revolving speed 100-150 r/min obtained primary seed solution；By level-one
Seed liquor is inoculated into second order fermentation culture medium by the inoculum concentration of 5-15%, in 30-40 DEG C shaking table culture 15-22 hours, revolving speed
The viable count of 100-150 r/min, fermentation liquid reach 1 × 109Stop fermentation when cfu/mL or more, obtains composite bacteria fermentation
Liquid.
The preparation of the wall material aqueous solution, propylene glycol alginate is dissolved in the water, and being configured to mass fraction is 25%-
The Gluten range of hydrolysed peptides of 10%-20% is added in 35% aqueous solution, and wall material aqueous solution is made.
The preparation of the glue microcapsules, compound micro-ecological preparation stoste is as core material, by sodium alginate and Gluten water
Solve peptide and be used as wall material, be dispersed in the vegetable oil of Tween 80 as oily phase, add calcium chloride as curing agent, using emulsion process into
Row reaction, discards supernatant after centrifugation, obtains gluey microcapsules.
The vacuum freeze-drying, vacuum freeze drier is at -60 DEG C -- and it 50 DEG C, is lyophilized 24-36 hours, it is compound to obtain microcapsules
Probiotics.
The piglet feed is the mash feed or pellet for meeting sucking pig nutritional need, wherein microcapsules additive amount
For 0.3-1%.
Compared with prior art, the beneficial effects of the present invention are:
1. the present invention joined compound microecological microcapsules in piglet feed, core material is complex as with multi-cultur es, alginic acid the third two
Acid esters and Gluten range of hydrolysed peptides are wall material；Alginic acid malonate can not only be resistant to stomach acidic environment, and it is steady to improve microcapsules
It is qualitative, spawn activity is effectively saved, and can discharge rapidly in enteron aisle, composite bacteria is made to play maximum effectiveness.Meanwhile seaweed
Sour malonate itself is a kind of effective emulsifier, and addition added emulsifier is no longer needed to when preparing microcapsules, simplifies technique
And cost reduces.
2. joined Gluten range of hydrolysed peptides in microcapsule wall material of the invention, because it has control cholesterol, blood glucose and blood
The effect of pressure, can be used as nutrition fortifier.Gluten range of hydrolysed peptides is in usually delicate flavour, can play because of glutamine content height simultaneously
Attractant effect increases feed palatability.
3. piglet feed of the invention, reasonable nutritional arrangment, without any hormonal components, nutritive value is high, there is enhancing cream
The immune and disease-resistant ability of pig.
Specific embodiment
As previously mentioned, inventor is studied for a long period of time and largely practiced in view of prior art defect, it is able to propose this hair
Bright technical solution.In order to make those skilled in the art more fully understand technical solution of the present invention, below with reference to most
The present invention is described in further detail for good embodiment.
Embodiment 1
A kind of sucking pig early-stage fodder of enteric solubility compound micro-ecological preparation specifically comprises the following steps: corn 55%, soybean concentration
Albumen 5%, rice protein 22%, whey powder 6%, grease 6%, other include the premix of vitamin, trace quantity mineral substance element and calcium phosphorus etc.
Material 5%, microcapsules probiotics 1%.The microcapsules core material is that Bifidobacterium, lactobacillus bulgaricus, Lactobacillus delbrueckii are multiple
Combined bacteria liquid, the wall material of microcapsules are propylene glycol alginate；The core material and wall material ratio of the microcapsules are 0.05:1.It will be micro-
Capsule wall material is configured to the aqueous solution that mass concentration is 30%, and addition compound micro-ecological preparation, which is uniformly mixed, is made mixed liquor, then
Calcium chloride is added as coagulator, gluey microcapsules are made, is sent into vacuum freeze-drier and microscapsule powder is made.It finally will be newborn
Pannage component and microscapsule powder are mixed, and are transferred to pulverizer and are crushed, and 40 mesh standard sieves are crossed, using conventional feed
The particle that partial size is 2-5mm is made to get a kind of piglet feed of enteric solubility compound micro-ecological preparation in processing method.
Embodiment 2
A kind of piglet feed of enteric solubility compound micro-ecological preparation, specifically comprises the following steps: corn 55%, dregs of beans 26%, fish meal
6%, whey powder 3%, grease 3.5%, wheat bran 2%, other include the premix 5% of vitamin, trace quantity mineral substance element and calcium phosphorus etc., micro-
0.5 part of capsule.The microcapsules core material be Bifidobacterium, lactobacillus bulgaricus, Lactobacillus delbrueckii composite bacteria liquid, microcapsules
Wall material is propylene glycol alginate；The core material and wall material ratio of the microcapsules are 0.1:1.Microcapsule wall material is configured to matter
Concentration is measured as 35% aqueous solution, addition compound micro-ecological preparation, which is uniformly mixed, is made mixed liquor, and calcium chloride is then added and is used as and coagulates
Gu gluey microcapsules are made in agent, it is sent into vacuum freeze-drier and microscapsule powder is made.Finally by piglet feed component and micro- glue
Capsule powder is mixed, and is transferred to pulverizer and is crushed, and 40 mesh standard sieves are crossed, and being put into feed granulator and partial size is made is 2-
The particle of 5mm to get a kind of enteric solubility compound micro-ecological preparation piglet feed.
In addition, inventor also refers to the mode of Examples 1 to 2, with the other raw materials and item listed in this specification
Part etc. is tested, and the piglet feed of enteric solubility compound micro-ecological preparation at low cost, that product quality is high has equally been made.
It should be appreciated that the technical concepts and features of above-described embodiment only to illustrate the invention, its object is to allow be familiar with this
The personage of item technology cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all
Equivalent change or modification made by Spirit Essence according to the present invention, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of piglet feed preparation method of compound micro-ecological preparation microcapsules, which is characterized in that Bifidobacterium, Bao Jiali
The strains such as sub- lactobacillus, Lactobacillus delbrueckii, Pediococcus pentosaceus carry out mixing fermentation culture, obtain compound micro-ecological preparation stoste and make
The vegetable oil of tween 80 is dispersed in as oil using sodium alginate propylene glycol ester and Gluten range of hydrolysed peptides as wall material for core material
Phase is added calcium chloride as curing agent, is reacted using emulsion process, discarded supernatant after centrifugation, obtain gluey microcapsules, vacuum
Freeze-drying, obtains compound micro-ecological preparation microcapsules.
2. the piglet feed according to claim 1 preparation method of compound micro-ecological preparation microcapsules, it is characterised in that
The viable bacteria of the strains such as Bifidobacterium, lactobacillus bulgaricus, Lactobacillus delbrueckii, Pediococcus pentosaceus in the compound micro-ecological preparation
Number is all larger than 1 × 108 CFU/mL, obtained compound micro-ecological preparation microcapsules are spherical in shape or oblate, and partial size is between 30-50 μm
Between.
3. the piglet feed according to claim 1 preparation method of compound micro-ecological preparation microcapsules, it is characterised in that
The multiple bacteria compound fermentation culture is inoculated with various probiotics into fluid nutrient medium, small in 30-40 DEG C of shaking table culture 12-24
When, revolving speed 100-150 r/min obtains seed liquor；Seed liquor is inoculated into first order seed by the inoculum concentration of inoculum concentration 5-15%
In culture medium, in mutually synthermal culture 6-10 hours, revolving speed 100-150 r/min obtained primary seed solution；By primary seed solution
Be inoculated into second order fermentation culture medium by the inoculum concentration of 5-15%, in 30-40 DEG C shaking table culture 15-22 hours, revolving speed 100-150
The viable count of r/min, fermentation liquid reach 1 × 109Stop fermentation when cfu/mL or more, obtains composite bacteria fermentation liquid.
4. the piglet feed according to claim 1 preparation method of compound micro-ecological preparation microcapsules, it is characterised in that
The piglet feed is the mash feed or pellet for meeting sucking pig nutritional need.
5. the piglet feed according to claim 1 preparation method of compound micro-ecological preparation microcapsules, it is characterised in that
The preparation method of the piglet feed, mass component are as follows: 0.3-1 parts of microcapsules, the core material of the microcapsules is composite bacteria
Probiotics, wall material are sodium alginate propylene glycol ester and Gluten range of hydrolysed peptides；The core material of the microcapsules and the ratio of wall material
Example are as follows: 0.05-0.15:1.
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| CN111493322A (en) * | 2020-05-15 | 2020-08-07 | 苏州普瑞森基因科技有限公司 | Microencapsulated probiotic bacteria and preparation method and application thereof |
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Application publication date: 20190329 |