CN109234263A - A method of the crosslinked action immobilized enzyme based on adenosine monophosphate and graphene - Google Patents
A method of the crosslinked action immobilized enzyme based on adenosine monophosphate and graphene Download PDFInfo
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- CN109234263A CN109234263A CN201811127745.9A CN201811127745A CN109234263A CN 109234263 A CN109234263 A CN 109234263A CN 201811127745 A CN201811127745 A CN 201811127745A CN 109234263 A CN109234263 A CN 109234263A
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01007—Peroxidase (1.11.1.7), i.e. horseradish-peroxidase
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- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
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Abstract
The invention discloses a kind of methods of crosslinked action immobilized enzyme based on adenosine monophosphate and graphene comprising following steps: (1) adenosine monophosphate being dissolved in hydroxyethyl piperazine second sulfuric acid solution；(2) fixed enzyme will be needed to be dissolved in ZnCl2In solution；(3) step (1) and (2) two kinds of solution are mixed；(4) graphene solution of low concentration is added into the mixed solution of step (3), graphene and adenosine monophosphate form stable gel structure by crosslinked action power；(5) it mixes and carries out centrifugal treating after standing, finally obtain the enzyme being fixed on gel structure.The gel structure that the present invention is formed by adenosine monophosphate with graphene its efficiently enzyme can be fixed, the present invention is in fixing glucose oxidase, lipase from candida sp, during horseradish peroxidase and alpha-amylase, efficiently enzyme can be fixed, the bioactivity for keeping enzyme simultaneously, improves its sensitivity.
Description
Technical field
The present invention relates to catalysis material synthesis field more particularly to a kind of crosslinking works based on adenosine monophosphate and graphene
With the method for immobilized enzyme.
Background technique
Fixation techniques for enzyme research starts from 1910, formal research in the 1960s, the seventies in the whole world
Generally carry out.Enzyme immobilizatio (Immobilization of enzymes) is to fetter or be limited to one for enzyme with solid material
Determine in region, can still provide for a kind of technology that its distinctive catalysis reacts and can be recycled and reuse.Compared with resolvase,
Immobilised enzymes overcomes the deficiency of resolvase keep its efficiently single-minded and mild enzymic catalytic reaction characteristic while
Storage stability height is presented in place, separation and recovery is easy, can repeatedly use, it is a series of to operate continuously controllable, simple process etc.
Advantage.Immobilized enzyme is not only extremely living in the research of the ambits such as chemical, biology and bioengineering, medicine and life science
Jump, is rapidly developed and is widely applied, and because has the Impacts on ecology and environment for saving the energy, reducing or preventing and remedying pollution
And meet the strategic requirement of sustainable development.Enzyme through immobilization has stability high compared with resolvase, and recycling is convenient.It is easy to
Control, can Reusability, it is low in cost the advantages that.In biological industry, medicine and clinical diagnosis, chemical analysis, environmental protection, energy
Source exploitation and basic research etc. have played important function.
The fixing means of enzyme has physical method and chemical method two major classes.Physical method includes physisorphtion, investment etc..Object
The advantages of logos immobilized enzyme, is that enzyme does not participate in chemical reaction, and overall structure remains unchanged, and the catalytic activity of enzyme is protected very well
It stays.It is not applicable to some reactions but since embedded object or half suitable film have certain space or steric hindrance effect.
Chemical method is to be keyed to enzyme on natural or synthesis macromolecule carrier by chemistry, passes through enzyme surface using coupling agent
Group gets up enzyme crosslinking, and the method for forming bigger, insoluble immobilised enzymes in average molecular.
But the preparation process complexity of existing immobilized enzyme is cumbersome, and preparation cost is higher, is unfavorable for the extensive of immobilized enzyme
Production and commercialization, and the stability of the dielectric material of existing absorption immobilized enzyme is lower, is unfavorable for improving the activity of enzyme.
Summary of the invention
To solve defect in the prior art, the present invention provides a kind of crosslinked action based on adenosine monophosphate and graphene
The method of immobilized enzyme can efficiently fix enzyme, while keep the bioactivity of enzyme, improve the active and sensitive of enzyme
Degree.
It is specific itself the following steps are included:
(1) adenosine monophosphate is dissolved in hydroxyethyl piperazine second sulfuric acid solution；
(2) fixed enzyme will be needed to be dissolved in ZnCl2In solution；
(3) step (1) and (2) two kinds of solution are mixed；
(4) graphene solution of low concentration is added into the mixed solution of step (3), graphene passes through with adenosine monophosphate
Crosslinked action power forms stable gel structure；
(5) it mixes and carries out centrifugal treating after standing, finally obtain the enzyme being fixed on gel structure.
Preferably, the enzyme is glucose oxidase, lipase from candida sp, horseradish peroxidase or alphalise starch
Enzyme.
Preferably, concentration is 10- by described be dissolved in adenosine monophosphate in hydroxyethyl piperazine second sulfuric acid solution specially
It is 10-30mM that the adenosine monophosphate of 35mM, which is dissolved in concentration, and pH value is in the hydroxyethyl piperazine second sulfuric acid solution of 7-7.5.
Preferably, fixed enzyme will be needed to be dissolved in ZnCl2It is specially to be dissolved in the enzyme that concentration is 0.01-0.2mM in solution
The ZnCl of concentration 10-75mM2In solution.
Preferably, its concentration of the graphene solution of the low concentration is 0.01-0.1mg/mL.
Preferably, it carries out standing 1-2 hours after centrifugal treating specially mixes after the mixing is stood, then with 6000-
The revolving speed centrifugal treating 10-20min of 8000rpm.
Preferably, the adenosine monophosphate is the ester that phosphoric acid and nucleosides adenosine are formed, by phosphate functional group, pentose core
Sour sugar and bases adenine are formed.
Preferably, the graphene is graphene oxide, can be carried out by the way that expansible graphite to be placed in crucible
It is added to after microwave treatment in dispersing agent and is ultrasonically treated to obtain slurry, centrifugal treating then is carried out to slurry, slurry is divided into
Upper layer and lower layer remove supernatant liquor, and graphene oxide is prepared.
Preferably, described to be made by adenosine triphyosphate by hydrolysis twice by adenosine monophosphate.
The invention adopts the above technical scheme, the gel structure formed by adenosine monophosphate with graphene its can be efficient
Enzyme is fixed, adenosine monophosphate is a kind of biological micromolecule, will not influence the activity of enzyme, graphite during immobilized enzyme
Alkene is also a kind of inorganic material that biocompatibility is splendid, therefore the present invention is in fixing glucose oxidase, Candida fat
During enzyme, horseradish peroxidase and alpha-amylase, efficiently enzyme can be fixed, while keeping the biology of enzyme living
Property, improve its sensitivity.
Detailed description of the invention
Invention is further explained with reference to the accompanying drawing:
Fig. 1 is method flow schematic diagram of the invention；
Fig. 2 is the chemical structure schematic diagram of adenosine monophosphate of the present invention；
The microcosmic schematic diagram of gel three-dimensional structure that Fig. 3 is cross-linked to form between adenosine monophosphate of the present invention and graphene.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
As shown in Figure 1, the invention discloses a kind of sides of crosslinked action immobilized enzyme based on adenosine monophosphate and graphene
Method comprising following steps:
S101: adenosine monophosphate is dissolved in hydroxyethyl piperazine second sulfuric acid solution；
S102: fixed enzyme will be needed to be dissolved in ZnCl2In solution；
S103: both the above solution is mixed；
S104: the graphene solution of low concentration being added into above-mentioned mixed solution, and graphene and adenosine monophosphate pass through friendship
Connection active force forms stable gel structure；
S105: it mixes and carries out centrifugal treating after standing, finally obtain the enzyme being fixed on gel structure.
It can specifically be realized by following embodiment:
Embodiment 1
The adenosine monophosphate of 10mM is dissolved in concentration for 10mM, in the hydroxyethyl piperazine second sulfuric acid solution that pH value is 7, such as
Shown in Fig. 2, the adenosine monophosphate is the ester that phosphoric acid and nucleosides adenosine are formed, by phosphate functional group, pentose nucleic acid sugar and
Bases adenine is formed；Fixed enzyme will be needed to be dissolved in ZnCl2Specially the enzyme that concentration is 0.01mM is dissolved in solution dense
Spend the ZnCl of 10mM2In solution.Into above-mentioned mixed solution be added concentration be 0.01mg/mL graphene solution, graphene with
Adenosine monophosphate forms stable gel structure by crosslinked action power, and structure is as shown in Figure 3；It is stood after mixed liquor is mixed
1 hour, then with the revolving speed centrifugal treating 20min of 6000rpm, finally obtain the enzyme being fixed on gel structure.
Wherein, the enzyme is lipase from candida sp, and the graphene is graphene oxide, can be by that will may expand
Graphite is placed in crucible carry out microwave treatment after be added in dispersing agent and be ultrasonically treated to obtain slurry, then to slurry into
Row centrifugal treating, slurry are divided into upper layer and lower layer, remove supernatant liquor, and graphene oxide is prepared.It is described by adenosine monophosphate
It is made by adenosine triphyosphate by hydrolysis twice.
Embodiment 2
It unlike the first embodiment, is that the adenosine monophosphate of 35mM is dissolved in concentration for 30mM in the present embodiment, pH value is
In 7.5 hydroxyethyl piperazine second sulfuric acid solution.Fixed enzyme will be needed to be dissolved in ZnCl2It is specially to be in solution by concentration
The enzyme of 0.2mM is dissolved in the ZnCl of concentration 75mM2In solution.The graphene that concentration is 0.1mg/mL is added into above-mentioned mixed solution
Solution, graphene and adenosine monophosphate form stable gel structure by crosslinked action power；It is small that 2 are stood after mixed liquor is mixed
When, then with the revolving speed centrifugal treating 10min of 8000rpm, finally obtain the enzyme being fixed on gel structure.Wherein, the enzyme is
Horseradish peroxidase.
The invention adopts the above technical scheme, and adenosine monophosphate also known as a phosphorus adenylate, 5'- adenylic acid are one
The nucleotide that kind is found in ribonucleic acid (RNA).It is the ester of a kind of phosphoric acid and nucleosides adenosine, and by phosphate functional group,
Pentose nucleic acid sugar and bases adenine are formed, and are to be hydrolyzed to obtain twice by adenosine triphyosphate.Adenosine monophosphate with
Inorganic nano material, metal cation can be self-assembly of stable gel structure in aqueous solution, can be used for fixed egg
White enzyme horseradish peroxidase.
And graphene has excellent electric conductivity, can be applied in electrochemica biological sensor, accelerates electronics in electricity
The conduction velocity of pole surface, improves the electric conductivity of modified electrode, to enhance the response signal of electrochemistry, it is sensitive to improve detection
Degree；On the other hand, compared with other common nano materials, the big specific surface area of graphene sheet layer is the absorption of biomolecule
More binding sites are provided, and can be repaired by π-π effect or other hydrophobic interactions in conjunction with various biomolecules
Adorn electrode.
It can efficiently fix enzyme the gel structure that the present invention is formed by adenosine monophosphate with graphene, single phosphorus
Adenosine monophosphate is a kind of biological micromolecule, will not influence the activity of enzyme during immobilized enzyme, and graphene is also a kind of biofacies
The splendid inorganic material of capacitive, therefore the present invention is in fixing glucose oxidase, lipase from candida sp, horseradish peroxidase
And during alpha-amylase, efficiently enzyme can be fixed, while keeping the bioactivity of enzyme, improve its sensitivity.
Claims (9)
1. a kind of method of the crosslinked action immobilized enzyme based on adenosine monophosphate and graphene, which is characterized in that including following step
It is rapid:
(1) adenosine monophosphate is dissolved in hydroxyethyl piperazine second sulfuric acid solution；
(2) fixed enzyme will be needed to be dissolved in ZnCl2In solution；
(3) step (1) and (2) two kinds of solution are mixed；
(4) graphene solution of low concentration is added into the mixed solution of step (3), graphene and adenosine monophosphate are by being crosslinked
Active force forms stable gel structure；
(5) it mixes and carries out centrifugal treating after standing, finally obtain the enzyme being fixed on gel structure.
2. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
The enzyme is glucose oxidase, lipase from candida sp, horseradish peroxidase or alpha-amylase.
3. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
Described be dissolved in adenosine monophosphate is specially the adenosine monophosphate for being 10-35mM by concentration in hydroxyethyl piperazine second sulfuric acid solution
Being dissolved in concentration is 10-30mM, and pH value is in the hydroxyethyl piperazine second sulfuric acid solution of 7-7.5.
4. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
Fixed enzyme will be needed to be dissolved in ZnCl2It is specially that the enzyme that concentration is 0.01-0.2mM is dissolved in concentration 10-75mM's in solution
ZnCl2In solution.
5. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
Its concentration of the graphene solution of the low concentration is 0.01-0.1mg/mL.
6. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
The mixing carries out centrifugal treating after standing be specially to stand 1-2 hours after mixing, then be centrifuged with the revolving speed of 6000-8000rpm
Handle 10-20min.
7. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
The adenosine monophosphate is the ester that phosphoric acid and nucleosides adenosine are formed, fast by phosphate functional group, pentose nucleic acid sugar and base gland
Purine is formed.
8. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
The graphene is graphene oxide, can be added to by the way that expansible graphite to be placed in crucible after progress microwave treatment
It is ultrasonically treated to obtain slurry in dispersing agent, centrifugal treating then is carried out to slurry, slurry is divided into upper layer and lower layer, removes upper layer
Graphene oxide is prepared in clear liquid.
9. the method for the crosslinked action immobilized enzyme of adenosine monophosphate according to claim 1 and graphene, it is characterised in that:
It is described to be made by adenosine triphyosphate by hydrolysis twice by adenosine monophosphate.
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Cited By (2)
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| CN110441357A (en) * | 2019-08-30 | 2019-11-12 | 济南大学 | A kind of method of paper base bimodulus electrochemical sensor detection adenosine triphyosphate |
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Application publication date: 20190118 |