CN106668611B - Traditional Chinese medicine composition for promoting generation of brown adipose tissues and preparation method thereof - Google Patents
Traditional Chinese medicine composition for promoting generation of brown adipose tissues and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for promoting generation of brown adipose tissues and treating obesity and a preparation method thereof. The traditional Chinese medicine composition is prepared from 3-50 parts of sea buckthorn, 3-30 parts of lonicera confusa, 3-50 parts of allium macrostemon, 10-50 parts of oyster, 3-30 parts of bunge cherry seed, 10-30 parts of endothelium corneum gigeriae galli, 3-30 parts of spina date seed, 3-30 parts of clove, 3-30 parts of kelp, 3-20 parts of Chinese torreya seed, 3-10 parts of seville orange flower, 3-30 parts of dry reed root, 3-20 parts of raspberry, 3-20 parts of yellow mustard seed, 3-30 parts of Chinese olive and 3-20 parts of chicory. The raw materials are both food and Chinese medicinal materials, and have the advantages of safety, easy obtainment, simple preparation method, low price and the like.
Description
Technology neighborhood
The invention relates to a traditional Chinese medicine composition for promoting generation of brown adipose tissues and treating obesity and a preparation method thereof.
Background
Obesity is a pathological condition in which the normal physiological functions of the body are damaged to different degrees due to the excessive accumulation of adipose tissues in the body. The etiology of the disease is complex, and it is thought to be related to many factors such as environment, genetics, physiology, metabolism, behavior and psychology, and obesity is usually diagnosed by Body Mass Index (BMI). Obesity seriously affects physical and mental health of human beings, is a risk factor of metabolic diseases such as type 2 diabetes mellitus and the like, chronic non-infectious diseases such as cardiovascular and cerebrovascular diseases, respiratory diseases, malignant tumors and the like, and is considered as one of main risk factors affecting health by the world health organization. With the rapid development of global economy and the change of life style, the world obesity problem becomes more serious. Over 15 billion overweight and obese people are currently present worldwide, with an estimated increase in overweight people to 21.6 billion and obese people to 11.2 billion by 2030. Therefore, the incidence and mortality of obesity-related complications will increase continuously, and the prevention and treatment of obesity is becoming more severe, and how to treat obesity safely and effectively becomes an urgent problem to be solved.
At present, the treatment of obesity mainly comprises 3 methods of changing life style, surgical treatment and drug treatment, wherein (1) the change of life style comprises diet therapy and participation in aerobic exercise, but the intervention of the life style is difficult to persist, and most people can only achieve transient success; (2) surgical treatments, including mainly Bariatric surgery, lipoplasty and intragastric balloon therapy, present significant risks, although surgery is currently the most effective treatment; (3) currently, the available weight-loss drugs are very limited, and chemicals rimonabant (rimonabant) and sibutramine (sibutramine) have exited the market of european unions. Therefore, currently, few effective measures are available for weight loss, and new methods for weight loss are urgently needed.
There are mainly 2 types of adipose tissues in mammals, namely White Adipose Tissue (WAT) and Brown Adipose Tissue (BAT). Wherein the white adipose tissue accounts for 10% of the normal healthy adult human body mass, and has the main function of storing fat to be used as a human body energy reservoir and a subcutaneous heat insulation pad; the brown fat is responsible for decomposing white fat causing obesity, converting the white fat into carbon dioxide, water and heat, and can accelerate metabolism, promote consumption of white fat, and prevent obesity. The heat generated by brown adipose tissue mainly depends on the activation of uncoupling protein 1(UCP1), UCP1 is a mitochondrial protein which is specifically present in brown adipose cells, and can metabolize fatty acid to generate energy after oxidative phosphorylation and uncoupling of mitochondria, which is very important for maintaining body temperature of an organism. Experimental studies in rodents have found that induction of white adipose tissue to brown adipose tissue transformation ("browning" of white adipose tissue) reduces the mass of the animal and increases the blood glucose homeostasis of the animal's body. Additional studies have shown that the amount of brown adipose tissue is significantly reduced in overweight and obese people, the amount of brown adipose tissue is inversely proportional to BMI, fat content, brown adipose tissue is indeed present and functional in adults, and obesity is also closely linked to the reduction in the amount of brown adipose tissue. The presence and physiological function of brown adipose tissue suggests a new strategy for the control of obesity. The method for increasing brown fat or promoting the conversion of white fat into brown fat by regulating the differentiation pathway of brown fat may become an effective method for treating obesity, and has become a hotspot of obesity research.
Disclosure of Invention
The invention provides a traditional Chinese medicine composition for promoting generation of brown adipose tissues and improving functions thereof so as to prevent and treat obesity and a preparation method thereof.
The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 3-50 parts of sea-buckthorn, 3-30 parts of lonicera confusa, 3-50 parts of allium macrostemon, 10-50 parts of oyster, 3-30 parts of bunge cherry seed, 10-30 parts of endothelium corneum gigeriae galli, 3-30 parts of spina date seed, 3-30 parts of clove, 3-30 parts of kelp, 3-20 parts of Chinese torreya seed, 3-10 parts of seville orange flower, 3-30 parts of dry reed root, 3-20 parts of raspberry, 3-20 parts of yellow mustard seed, 3-30 parts of Chinese olive and 3-20 parts of chicory.
The preferable mixture ratio is as follows: 20 parts of sea-buckthorn, 20 parts of lonicera confusa, 20 parts of allium macrostemon, 15 parts of oyster, 15 parts of bunge cherry seed, 15 parts of endothelium corneum gigeriae galli, 15 parts of spina date seed, 15 parts of clove, 15 parts of kelp, 10 parts of Chinese torreya seed, 10 parts of seville orange flower, 10 parts of dry reed rhizome, 10 parts of raspberry, 10 parts of yellow mustard seed, 10 parts of Chinese olive and 5 parts of chicory.
The traditional Chinese medicine composition can be used in medicines, health-care products or foods for preventing and treating obesity.
The preparation method of the traditional Chinese medicine composition comprises the following steps:
(1) pulverizing the raw materials, and mixing to obtain a mixture;
(2) extracting the mixture for 1-3 times by using 5-100% ethanol, wherein the material-liquid ratio is 3-30, the extraction time is 0.5-3 hours each time, and filtering and combining the extract;
(3) concentrating the combined extracting solution into an extract with the raw material content of 0.5-3 g/ml, adding auxiliary materials or auxiliary components commonly used in food or pharmacy, drying the extract, crushing, and preparing into food or a preparation form suitable for oral administration.
The composition can be prepared into jelly, tea, beverage, electuary, capsules, granules, chewable tablets, tablets or oral liquid preparations. Can also be used as food adjuvant or seasoning to achieve weight reducing effect.
A large number of pharmacological studies prove that the composition can promote the generation of brown adipose tissues, improve the expression of UCP1 protein of the brown adipose tissues to enhance the functions of decomposing white adipose tissues and generating heat of the brown adipose tissues of an obesity model mouse, obviously inhibit the abnormal growth of the body weight of the obesity model mouse, and can be used for preparing food, health-care food or medicaments for promoting the generation of the brown adipose tissues and enhancing the functions of the brown adipose tissues to prevent and treat obesity, and is used for preventing and treating the obesity in clinic. The raw materials are both food and Chinese medicinal materials, and have the advantages of safety, easy obtainment, simple preparation method, low price and the like.
Detailed Description
The present invention will be further described with reference to the following examples.
The percentages of solids in the solid mixture, liquids in the liquids, and solids in the liquids referred to in the examples are calculated as wt/wt, vol/vol, wt/vol, respectively, unless otherwise indicated.
Example 1: preparation method 1 of the traditional Chinese medicine composition
The preparation method comprises the following steps of primarily shearing and crushing sea buckthorn 20g, lonicera confusa 20g, allium macrostemon 20g, oyster 15g, bunge cherry seed 15g, endothelium corneum gigeriae galli 15g, spina date seed 15g, clove 15g, kelp 15g, Chinese torreya seed 10g, seville orange flower 10g, dry reed rhizome 10g, raspberry 10g, yellow mustard seed 10g, Chinese olive 10g and chicory 5g, crushing by a crusher, and uniformly mixing. Extracting the uniformly mixed medicinal materials of the composition with 15 times of 50% ethanol for 1 hour, filtering and collecting an extracting solution, adding 10 times of 50% ethanol for extraction for 1 hour for the second time, filtering and collecting the extracting solution, combining the two extracting solutions, concentrating into an extract with the raw material content of 2g/ml, drying the extract, and crushing.
Example 2: preparation method 2 of the traditional Chinese medicine composition
Taking 10g of sea-buckthorn, 10g of lonicera confusa, 10g of allium macrostemon, 5g of oyster, 5g of bunge cherry seed, 5g of endothelium corneum gigeriae galli, 5g of spina date seed, 5g of clove, 10g of kelp, 5g of Chinese torreya seed, 5g of seville orange flower, 10g of dry reed rhizome, 10g of raspberry, 3g of yellow mustard seed, 10g of Chinese olive and 5g of chicory, primarily cutting, crushing by a crusher, and uniformly mixing. Extracting the uniformly mixed medicinal materials of the composition for 1 hour by 15 times of 30% ethanol, filtering and collecting extract, adding 10 times of 30% ethanol for extraction for 1 hour for the second time, filtering and collecting extract, combining the two extracts, concentrating into extract with the raw material content of 1.5g/ml, adding food additives, and adding a proper amount of purified water to obtain the beverage of the traditional Chinese medicine composition.
Example 3: preparation method 3 of the traditional Chinese medicine composition
The preparation method comprises the following steps of primarily shearing and crushing sea-buckthorn 20g, lonicera confusa 20g, allium macrostemon 10g, oyster 10g, bunge cherry seed 10g, endothelium corneum gigeriae galli 10g, spina date seed 15g, clove 10g, kelp 10g, Chinese torreya seed 10g, seville orange flower 5g, dry reed rhizome 15g, raspberry 10g, yellow mustard seed 5g, Chinese olive 10g and chicory 20g, crushing by a crusher, and uniformly mixing. Extracting the uniformly mixed medicinal materials of the composition for 1 hour by using 15 times of 60% ethanol, filtering and collecting extract, adding 10 times of 40% ethanol for extraction for 1 hour for the second time, filtering and collecting extract, combining the two extracts, concentrating the extract into extract with the raw material content of 2g/ml, adding a proper amount of starch, drying, crushing, adding a proper amount of microcrystalline cellulose, uniformly mixing, and preparing the capsule of the traditional Chinese medicine composition.
Example 4: the traditional Chinese medicine composition has the effects on the weight, the generation of brown fat and the expression of UCP1 protein of an obesity model mouse
1. Experimental animals:
male C57BL/6J mice 4-5 weeks old, SPF grade, mean body mass (14.9 ± 0.8) g, provided by the Guangdong provincial medical laboratory animal center, animal certification No.: SCXK (Yue) 2013-.
2. The experimental method comprises the following steps:
2.1 preparation of the Chinese medicinal composition of the present invention
200g of sea-buckthorn, 200g of lonicera confusa, 200g of allium macrostemon, 150g of oyster, 150g of bunge cherry seed, 150g of endothelium corneum gigeriae galli, 150g of spina date seed, 150g of clove, 150g of kelp, 100g of Chinese torreya seed, 100g of seville orange flower, 100g of dry reed rhizome, 100g of raspberry, 100g of yellow mustard seed, 100g of Chinese olive and 50g of chicory are taken, subjected to primary shearing, crushed by a crusher and uniformly mixed. Extracting the uniformly mixed medicinal materials of the composition with 15 times of 50% ethanol for 1 hour, filtering and collecting the extracting solution, adding 10 times of 50% ethanol for extraction for 1 hour for the second time, filtering and collecting the extracting solution, combining the two extracting solutions, and concentrating into an extract for the pharmacological research.
2.2 animal model establishment and grouping
70 mice were randomly divided into a normal control group (n ═ 10) and an obesity model group (n ═ 60), the normal control group was fed with a normal diet, and the obesity model group was fed with a high-fat diet (containing 79% of basal diet, 10% of lard, 10% of egg yolk powder, and 1% of cholesterol). After 10 weeks of feeding, individuals with a body mass of 20% higher than the average body mass of the normal control group mice were selected as obese model animals. In the experiment, 46 obese model mice are successfully induced (the success rate is 76.7%), and the obese model mice are divided into an obese model control group (12 mice), a composition low dose group (12 mice), a composition medium dose group (12 mice) and a composition high dose group (10 mice) according to a random digital table method. The normal control group and the obesity model control group are respectively administered with distilled water with equal volume by gavage for 1 time/day for 6 weeks, and the normal control group and the obesity model control group are continuously fed with high-fat feed for 1 time/day, and meanwhile, the composition low-dose group, the composition medium-dose group and the composition high-dose group are respectively administered with gavage with 0.1ml/10g of the suspension of the composition of the invention with the concentration of 0.7g crude drug/ml, 1.4g crude drug/ml and 2.8g crude drug/ml.
2.3 index monitoring
After the last administration, animals were fasted overnight, fasted for fasting body mass weighing and recording in the morning of day 2, then the animals were anesthetized, sacrificed by decapitation, the interscapular brown adipose tissue was rapidly taken, the attached muscle and white adipose tissue were removed, weighed and recorded. Subsequently, the brown adipose tissues were fixed in 10% formalin and immunohistochemical staining was performed by ABC method according to the instruction of immunohistochemical staining kit. Stained sections were visualized under 200-fold microscope, each section taken 10 fields at random, and the optical density values (IOD) of UCP1 positive expression were analyzed using Image-Pro Plus 6.0 software.
3. The experimental results are as follows:
as can be seen from table 1, compared to the normal control group, the body mass of the mice in the obesity model control group was significantly increased (P <0.01), the brown fat weight was significantly decreased (P <0.05), the brown fat weight/body weight was significantly decreased (P <0.01), and the UCP1 protein expression was significantly decreased (P < 0.01). The results suggest that the high fat feed is fed to obesity model mice to cause abnormal increase of body mass, reduction of generation of brown adipose tissue, decomposition of brown adipose tissue and reduction of thermogenesis function. Compared with the obesity model group, the mice in the low, medium and high dose groups of the composition have significantly reduced body mass (P <0.05 or 0.01), significantly increased brown fat weight/body weight (P <0.05 or 0.01) and significantly increased UCP1 protein expression (P <0.05 or 0.01). The composition can promote the generation of the brown fat of an obesity model mouse, improve the expression of UCP1 protein of a brown adipose tissue so as to enhance the functions of decomposing white adipose tissue and generating heat of the brown adipose tissue of the obesity model mouse and obviously inhibit the abnormal increase of the body weight of the obesity model mouse.
TABLE 1 Effect of the compositions of the present invention on mouse body mass, brown adipose tissue weight and UCP1 protein expression
Note: comparison with normal control group:##p is less than 0.01; comparison with model control group:*P＜0.05**P＜0.01
according to the theory of traditional Chinese medicine formula, the composition with the same or similar effect is changed on the basis of the formula of the invention, and the invention belongs to the protection scope.
Claims (4)
1. The traditional Chinese medicine composition for promoting the generation of brown fat and improving the thermogenesis function is characterized by being prepared from the following raw materials in parts by weight: 3-50 parts of sea-buckthorn, 3-30 parts of lonicera confusa, 3-50 parts of allium macrostemon, 10-50 parts of oyster, 3-30 parts of bunge cherry seed, 10-30 parts of endothelium corneum gigeriae galli, 3-30 parts of spina date seed, 3-30 parts of clove, 3-30 parts of kelp, 3-20 parts of Chinese torreya seed, 3-10 parts of seville orange flower, 3-30 parts of dry reed root, 3-20 parts of raspberry, 3-20 parts of yellow mustard seed, 3-30 parts of Chinese olive and 3-20 parts of chicory.
2. The traditional Chinese medicine composition for promoting the generation of brown fat and improving the thermogenesis function is characterized by being prepared from the following raw materials in parts by weight: 20 parts of sea-buckthorn, 20 parts of lonicera confusa, 20 parts of allium macrostemon, 15 parts of oyster, 15 parts of bunge cherry seed, 15 parts of endothelium corneum gigeriae galli, 15 parts of spina date seed, 15 parts of clove, 15 parts of kelp, 10 parts of Chinese torreya seed, 10 parts of seville orange flower, 10 parts of dry reed rhizome, 10 parts of raspberry, 10 parts of yellow mustard seed, 10 parts of Chinese olive and 5 parts of chicory.
3. The use of the Chinese medicinal composition of claim 1 or 2 in the preparation of a medicament for the prevention and treatment of obesity.
4. The Chinese medicinal composition as claimed in claim 1 or 2, which is prepared by a method comprising the steps of:
(1) pulverizing the raw materials, and mixing to obtain a mixture;
(2) extracting the mixture for 1-3 times by using 5-100% ethanol, wherein the ratio of material to liquid is 1: 10-15 each time, the extraction time is 0.5-3 hours each time, filtering the extract, and then combining the extract;
(3) concentrating the combined extracting solution into an extract with the raw material content of 0.5-3 g/ml, adding pharmaceutically common auxiliary materials or auxiliary components, drying the extract, crushing, and preparing into a preparation form suitable for oral administration.
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| CN1732916A (en) * | 2004-08-09 | 2006-02-15 | 李川明 | Compound medical health-caring preparation containing procyanidins and other functional ingredients |
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| CN1732916A (en) * | 2004-08-09 | 2006-02-15 | 李川明 | Compound medical health-caring preparation containing procyanidins and other functional ingredients |
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| CN105497620A (en) * | 2015-12-24 | 2016-04-20 | 兰州古驰生物科技有限公司 | Health-care traditional Chinese medicine preparation for weight reduction and body building |
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