CN104582849B - Multiple reaction chambers in testing cassete - Google Patents

Multiple reaction chambers in testing cassete Download PDF

Info

Publication number
CN104582849B
CN104582849B CN201380034569.3A CN201380034569A CN104582849B CN 104582849 B CN104582849 B CN 104582849B CN 201380034569 A CN201380034569 A CN 201380034569A CN 104582849 B CN104582849 B CN 104582849B
Authority
CN
China
Prior art keywords
liquid
test chamber
test
chamber
access road
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380034569.3A
Other languages
Chinese (zh)
Other versions
CN104582849A (en
Inventor
R·布鲁吉博特
J·卡雷拉法布拉
A·科门格斯卡萨斯
J·A·加西亚桑切斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stat Diagnostica and Innovation SL
Original Assignee
Stat Diagnostica and Innovation SL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stat Diagnostica and Innovation SL filed Critical Stat Diagnostica and Innovation SL
Priority to CN201610103877.2A priority Critical patent/CN105642378B/en
Publication of CN104582849A publication Critical patent/CN104582849A/en
Application granted granted Critical
Publication of CN104582849B publication Critical patent/CN104582849B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Plasma & Fusion (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Testing Of Devices, Machine Parts, Or Other Structures Thereof (AREA)

Abstract

The present invention provides a kind of fluid test system, and it includes multiple test chamber, multiple access road and access road is connected to the fluid network of other chamber one or more.Each test chamber in multiple test chambers is characterised by length and hydraulic diameter.The length of each test chamber is generally parallel directed at gravitational vectors.Each test chamber has only one opening, and this opening is arranged along the length of corresponding test chamber.It addition, the only one access road that each test chamber is connected in multiple access road via its corresponding opening.

Description

Multiple reaction chambers in testing cassete
Technical field
Embodiments of the invention relate to clinical diagnosis field of tool.
Background technology
In view of molecular testing and the complexity of the automatization of immunoassay, lack and foot is provided Enough performances are so as to be used for the product close to patient's testing setup.Typical molecular testing bag Include various procedures, crack to extract DNA including the correct dose of reagent, Sample introduction, cell Or RNA, purification step and amplification are for its follow-up detection.Even if existing and making these During central laboratory's robot platform of some automatization, but for requiring short turnover For many tests of time, central laboratory can not provide knot in required time requirement Really.
But, it is difficult to provide accurate reliable results with rational cost in clinical settings System.In view of the complex characteristics of various molecular testing technology, without being carefully controlled If test parameter or environmental condition are undesirable, then error probably occurs in result.Example As, due to the external source reasons for its use of DNA, and make having for round pcr High entrance barrier is there is in instrument for clinical diagnostic applications.Feelings in special pathogen test Under condition, the main source of pollution is to perform in pipet, pipe or general laboratory equlpment The result of reaction before.It addition, use the molecular engineering detecting microbial pathogens possible Produce false negative.False negative may be derived from such as: processes suppression polymerase chain improperly anti- Answer the reagent of (PCR), such as hemoglobin, urine or expectorant；Less efficiently discharge from cell DNA；Or less efficiently extract and purification DNA or RNA.
Molecular engineering has abnormal sensitive level under the concentration lower than reference method before The fact makes to be very difficult to while avoiding false positive error calls obtain clinically relevant knot Opinion.In order to minimize this problem, especially for detection pathogen microorganism, test should There is quantization ability.Therefore, have increasing need for performing multichannel test and hot-wire array, with combination Enough data draw the conclusion be sure oing.As an example, the survey of existing PCR-based One major limitation of examination is to carry out the amplification of different target gene simultaneously.Although it is all As the technology of microarray provides the most much higher road ability, but their major limitation is The speed obtaining result is low, and this has no positive impact generally for for case control.
Summary of the invention
The present invention provides a kind of fluid test system, and it includes multiple test chamber.Simultaneously flow Body controls each test position can reduce the testing time, and improves at multiple test positions it Between obtain the probability of repeatable result.
In one embodiment, single port fluid test system includes multiple test chamber, each Test chamber is characterised by length and hydraulic diameter.Each test chamber in multiple test chambers Room all has only one opening, and this opening is arranged along the length of corresponding test chamber.Fluid Test system also includes the first access road and multiple divided fluid stream element.Divided fluid stream element will Original liquid along the first access road flowing is diverted in multiple second access road.Multiple Each test chamber in test chamber is connected to multiple second entrance via its corresponding opening Only one the second access road in passage.
The present invention describes a kind of illustrative methods.The method includes: make the liquid edge of primary quantity The first access road flowing of single port fluid test system.The liquid of primary quantity is divided to Multiple second access roades, each second access road is connected to multiple test chamber, Qi Zhongduo Each test chamber in individual test chamber has the only one of the length setting along chamber and opens Mouthful.The method also includes: each test chamber is filled with the liquid of final quantity, each test Final quantity in chamber is substantially identical, and the sum of the liquid of the final quantity of each test chamber Liquid equal to primary quantity.
In another embodiment, fluid test system includes multiple test chamber, multiple entrance Passage and access road is connected to the fluid network of other chamber one or more.Test chamber Room each has length and hydraulic diameter.The length of each test chamber is with gravitational vectors substantially It is directed at abreast.Each test chamber has only one opening, and this opening is surveyed along correspondence The length of examination chamber is arranged.It addition, each test chamber is connected to via its corresponding opening Only one access road in multiple access roades.
The present invention describes another kind of illustrative methods.The method includes: flow liquid through multiple Access road, each access road is connected to multiple test chamber.The length of each test chamber Generally parallel it is directed at gravitational vectors, and each test chamber has only one and opens Mouthful, this opening is arranged along the length of chamber.The method also includes: by multiple test chambers Each test chamber be filled with liquid, until threshold quantity.The method also includes: pass through entrance Passage by liquid from each test chamber sucking-off, to leave scheduled volume in each test chamber Liquid.
The present invention describes another kind of illustrative methods.The method includes: make first liquid flow through First access road, the length that this first access road is connected to along test chamber is high first The first opening arranged at degree.The length of test chamber is generally parallel directed at gravitational vectors. The method also includes: test chamber is filled with first liquid, until first threshold amount.Pass through First liquid from test chamber sucking-off, and is left in test chamber by the first access road The first liquid of one scheduled volume.The method also includes: make second liquid flow through the second access road, This second access road is connected to second arranged at the second height along the length of test chamber Opening.Second height is more than the first height.The method also includes: test chamber is filled with Two liquid, until Second Threshold amount.By the second access road by second liquid from test chamber Sucking-off, and in test chamber, leave the second liquid of the second scheduled volume.
The present invention describes another kind of illustrative methods.The method includes: flow liquid through multiple Each passage in passage, until arranging liquid sensing region in each channel, thus The liquid of scheduled volume is set in each passage in multiple passages.The method also includes: make multiple The liquid of the only scheduled volume in each passage in passage is flowed into the phase coupled with each passage In the chamber answered.
Accompanying drawing explanation
The accompanying drawing of the part being incorporated herein and form description shows the enforcement of the present invention Example, and also it is used for explaining the principle of the present invention together with description, so that association area Technical staff can manufacture and use the present invention.
Fig. 1 is the schematic diagram of the testing cassete system according to an embodiment.
Fig. 2 shows the side view of the testing cassete system according to an embodiment.
Fig. 3 shows the test chamber according to an embodiment.
Fig. 4 A-C shows the operation of the test chamber according to an embodiment.
Fig. 5 shows the multiple test chambers according to an embodiment.
Fig. 6 A-C shows the another kind of operation of the test chamber according to an embodiment.
Fig. 7 shows the another kind of test chamber according to an embodiment.
Fig. 8 shows the multiple test chamber of the another kind according to an embodiment.
Fig. 9 shows the multiple test chamber of the another kind according to an embodiment.
Figure 10 shows the testing cassete system being in analyzer according to an embodiment.
Figure 11-14 shows the exemplary chamber fill method according to an embodiment.
Embodiment of the invention will be described with reference to drawings.
Detailed description of the invention
Although discussing specific structure and arrange, but it is to be understood that this being only used to figure Show descriptive purpose.Those skilled in the relevant art are it will be appreciated that without departing from the present invention's In the case of spirit and scope, other structure can be used and arrange.For related-art technology It is evident that the present invention can be also used for other application various for personnel.
It should be noted that in the description to " embodiment ", " embodiment ", " exemplary reality Execute example " etc. reference show that described embodiment can include specific feature, structure or characteristic, But each embodiment can include specific feature, structure or characteristic.Additionally, so Phrase be not necessarily related to identical embodiment.It addition, it is specific when describing relatively with embodiment When feature, structure or characteristic, in those skilled in the art's cognitive range, regardless of whether It is expressly recited, such feature, structure or characteristic can be realized with other embodiments relatively.
Embodiment as herein described relates to testing cassete system, and it is used for performing various molecular testing, Such as immunoassay, PCR, DNA hybridization etc..In one embodiment, testing cassete and execution All parts needed for such test are formed integrally as single disposable package piece.Testing cassete Can be configured to be analyzed by externally measured system, to provide to enter with in testing cassete The data that the reaction of row is correlated with.In one embodiment, testing cassete includes having transparent window Multiple test chambers, to utilize each test chamber to perform optical detection.
In one example, single testing cassete can be used to utilize the sample specified to perform immunity point Analysis array.Testing cassete comprises all necessary buffer agent, reagent and marks being maintained at and sealing in chamber Signing, to perform immunoassay, this sealing chamber is integrally coupled in this box.
In another example, single testing cassete can be used to perform PCR.DNA can be through It is purified from the remainder of sample by the filter being attached in testing cassete.Sample can lead to Crossing filter extrusion, DNA can be removed by the eluting liquid simultaneously separately stored, and DNA is taken in another chamber, to start temperature cycles process.
One major limitation of molecular diagnosis instrument is the problem relevant to pollutant, such as, intersect Pollution, carryover contamination etc..Embodiment as herein described substantially eliminates sample pair by design The pollution of instrument.
In one embodiment, testing cassete provides the self-contained liquid sealed during manufacture process. Reagent with sample not any part with environment or instrument contact.This feature pair of testing cassete Also it is important for many laboratorys and hospital, disposes safely after using at product These products.
According to an embodiment, in order to perform hot-wire array, testing cassete includes multiple test chamber, These test chambers are designed to be convenient for measuring the optics of the content in each test chamber Characteristic.Such as, each test chamber includes transparent window, to allow content therein Carry out fluorescence or absorbance research.It addition, the design of the fluid placement of each test chamber is permissible Each chamber is allowed to be filled into identical level while using single pumping source.
Describe and each parts of the testing cassete system including test chamber herein with reference to accompanying drawing Relevant further details.The diagram that should be appreciated that each physical unit is not restricted , without departing from the spirit or scope of the present invention, the phase that the description herein is specified Skilled person will appreciate that of field, pass rearranges or otherwise changes any parts Various modes.
Fig. 1 shows the exemplary testing cassete with test chamber array according to an embodiment System 100.Although in this article by the structure with reference to exemplary testing cassete system 100, but It will be recognized by those skilled in the art, the embodiment of test chamber as herein described may be used for Any amount of test system type and structure.
Testing cassete system 100 includes box housing 102.Other parts are equally considered to comprise In testing cassete system 100, such as analyzer module or various driving part, such as pump or add Hot device.
Box housing 102 includes multiple fluid passage, chamber and reservoir.Such as, box housing 102 Can include multiple storage chamber 116, these storage chambers can comprise will analyzed or PCR The various buffer agents used in scheme or other reagent.Storage chamber 116 can be pre-filled with Plurality of liquid so that end user need not testing cassete system 100 is being put into analyzer Storage chamber 116 was filled before in.In another example, reagent is being put into storage chamber It was lyophilizing before in room 116.Box housing 102 can also include the side along box housing 102 One or more process chamber 124a-b that face is connected with fluid passage.Process chamber 124a-b May be used for various process and/or garbage application.
According to an embodiment, sample is incorporated in box housing 102 via sample port 114. In one example, sample port 114 receives solid-state, semisolid or liquid sample.At another In individual embodiment, box housing 102 includes the more than one entrance guiding sample.
Each chamber and passage around box housing 102 can use covering 118,126,127 Seal with 128.Covering can be can the film of fluid in seal box housing 102. In another example, covering can be plastic front board.In one example, one or many Individual covering is transparent.It addition, one or more coverings can by thermal control, with Heating part in housing 102.
The testing cassete system 100 of integration allows user that sample is placed into such as sample port In 114, then testing cassete system 100 is put in analyzer.In many embodiment, Interact via with analyzer, including such as purification, crack, mix, bond, label And/or the pending reactions steps of detection all can perform in testing cassete system 100, and Need not any end user intervene.It addition, because all of liquid maintains a seal against In testing cassete system 100, so after completion of testing, testing cassete system 100 can be from dividing Parser removes, and carries out safe process, without contamination analysis device.
Fig. 2 shows the side view of the box housing 102 according to an embodiment.List box shell The explanation of body 102, to describe the feature being likely to be of on box housing 102, but should not limit Make setting or the dimensional characteristic of these features.
Fig. 2 shows fluid network and a series of ports extending in box housing 102.Fluid Network may be coupled to the storage chamber 116 of box housing 102 and/or processes in chamber 124a-b One or more.These chambers can be arranged on box housing 102 with the side shown in Fig. 2 On relative side.In one embodiment, fluid network is also connected to a series of test chamber 216。
Each fluid passage may be designed to terminate at port, and this port will be with housing The port on movable transfer module (not shown) or valve region in 102 have a common boundary.According to one Embodiment, multiple ports 210 allow fluid to flow to any chamber of housing 102.Multiple ports 210 may serve as the arrival end that liquid is inhaled in the internal chamber in box housing 102 Mouthful, or may serve as liquid and be discharged to the fluid network of box housing 102 from internal chamber Outlet port.Such as, liquid can be pressurized, to flow through the right number in fluid port 210 Second port, and be flowed in the most remote right side test chamber 216.Additionally, liquid is permissible It is sucked back into fluid port from the most remote right side test chamber 216 via the vacuum pressure applied In right several second port in 210.
The shape of test chamber 216 can be similar to such as centrifuge tube.In one embodiment, Liquid can be inhaled in test chamber 216, be loaded previously in each test chamber Reagent mixing.Such as, what each test chamber can be loaded with for PCR process is different Primer and probe, and liquid can be inhaled into each test chamber, with in each chamber Form different mixture.Reagent can be lyophilized before being loaded in test chamber 216. In another embodiment, test chamber 216 is additionally operable to sample detection.Can utilize and analyze External light source and photoelectric detector that device couples detect, and testing cassete system 100 is placed on this In analyzer.Therefore, any wall of test chamber 216 or covering can be transparent, with Allow to carry out optical detection.In one example, photoelectric detector is under one or more wavelength Measure the absorbance by the liquid in test chamber.In another example, photoelectric detector Measure the fluorescence signal generated by the fluorescent chemicals in test chamber.In one embodiment, Fluorescence measurement is carried out below test chamber 216.Test chamber 216 goes for other inspection Survey device, such as electrochemistry, electromechanics, surface plasmon resonance device etc..
According to an embodiment, in the upstream of test chamber 216 it is observed that one group less Passage expansion section 214.Passage expansion section 214 can serve as liquid sensing region.Therefore, logical Expansion section, road 214 can be used for detecting in passage expansion section 214 together with external optical probe Whether there is liquid.This determine other function that can be used to activate testing cassete system 100. In another embodiment, passage expansion section 214 can include the sensor of integration, such as Patterning electric resistance sensor, to indicate existence or the flow of fluid.It addition, expand at some passage The existence of the liquid in the corresponding test chamber 216 of optical signalling monitoring detected at big portion 214.
Liquid sensing region in passage expansion section 214 can be used to set to be metered to each The scheduled volume of the liquid in test chamber 216.Such as, liquid can individually or simultaneously by It is pressurized in each passage, until liquid arrives the liquid sensing region in each passage.So, Each passage can comprise the liquid of substantially identical amount, and this liquid filling channel is up to passage Expansion section 214.Afterwards, according to an embodiment, each passage can be individually or simultaneously It is pressurized to force the liquid of scheduled volume to be downwardly into each test chamber 216.
According to an embodiment, multiple chambers 231 that are pre-mixed can also be arranged on test chamber The upstream of 216.It is pre-mixed chamber 231 and can include dry chemical, that such as freeze or freeze Dry analyte.In another example, it is pre-mixed chamber 231 and includes dry chemistry pearl Or biological sample.Biological sample can carry out lyophilizing in being pre-mixed chamber 231.Such Biological or chemical compound can be stored in the most for a long time and be pre-mixed chamber 231 In.According to an embodiment, it is pre-mixed may be sized in order to spy of chamber 231 Not coordinating the size of dry chemistry pearl, general diameter is of about several millimeters.At an example In, it is attracted to the fluid of reaction chamber 216 and is stored in the sample being pre-mixed in chamber 231 Mixing.
According to an embodiment, at the low portion of box housing 102 in fig. 2, in test It is provided with optics below chamber 216 and enters region 240.Optics enters region 240 and is designed to It is all substantial transparent to all wavelengths used during optical detection process.At an example In, each single test chamber 216 has the optics of himself and enters region.At another In example, single optics enters region and extends across multiple test chamber 216.
According to an embodiment, the side of box housing 102 also illustrates that pressure port 236 With discharge port 234.According to an embodiment, pressure port 236 may be coupled to outside pressure Power source, for applying positive or negative pressure differential in the entire system.Discharge port 234 can To lead to air or to be connected to another pressure source.Such as, positive pressure difference can be applied to one Individual port, negative pressure differential is applied to another port simultaneously, so that liquid moves more quickly Passage by the connection of system.
One film or multiple film can be arranged on this series of test chamber 216.Film is permissible Sufficiently thin still to provide sufficiently sealing, also allow for the content in test chamber 216 simultaneously Carry out more easily via external source heating and/or cooling down.Such as, film can have by heat Any one or combinations thereof in electric installation, resistance heater and pressure air-flow and carry out thermal control The surface of system.In one example, film is polymeric film, and its thickness is less than 100 microns.? In one example, the thermal conductivity of film is more than 1W/mK.
Fig. 3 illustrates in greater detail the view of the test chamber 216 according to an embodiment.Survey Examination chamber 216 includes the single opening 304 arranged along the length of test chamber 216, and this is opened Mouth is connected to access road 302.Test chamber 216 also has curved bottom wall 306.The bending end Portion's wall 306 can be transparent, to allow to carry out optical detection below test chamber 216. Test chamber 216 has sufficiently large hydraulic diameter, is large enough so that action of gravity impact and surveys Fluid flowing in examination chamber 216.Therefore, test chamber 216 is aligned so that its length Substantially parallel with gravitational vectors.Due to this layout, liquid is affected by action of gravity, And filled chamber bottom-up.
Test chamber 216 can accommodate reagent 308.Reagent 308 can have any quantity Reagent.Reagent 308 can exist in liquid form, or is lyophilizing globule.Reagent 308 It is resuspended in being flowed in the liquid in test chamber 216.In another example, reagent 308 are stored in and are pre-mixed in chamber 231, in order to mix with the fluid of test chamber 216 upstream Close.
Access road 302 is connected to test chamber 216 via opening 304.Access road 302 The passage can being integrated in the multiple passages in box housing 102.Access road 302 There is provided and be used for what fluid was flowed in test chamber 216 and was sucked out from test chamber 216 Fluid path.
In one embodiment, the width width of the width ratio access road 302 of opening 304.Relatively Wide opening makes liquid enter test chamber 216 more controllably, and also reduces The size of the drop that liquid is produced by liquid when entering test chamber 216 by opening 304.This Each factor in a little factors reduces liquid between two lateral sidewalls of test chamber 306 Form the probability of meniscus.Meniscus is formed such that the amount of the liquid being difficult to control in chamber, And cause the formation of bubble.Bubble may destroy any life carried out in test chamber 216 Thing processing procedure, and cause optical measurement error.
According to an embodiment, Fig. 4 A-C shows the exemplary operation of test chamber 216. In Figure 4 A, during the liquid of scheduled volume is arranged on access road 302.The liquid of this scheduled volume It is assigned in test chamber 216 by opening 304, as shown in Figure 4 B.Liquid can be such as It is allocated via the pressure differential generated.
In one example, the amount being metered into the liquid in test chamber 216 is selected such that The liquid level of gained is at height h or lower section, and wherein h is that opening 304 is to test chamber Distance bottom 216.The liquid 402 of gained is as shown in Figure 4 C.Therefore, test chamber 216 Top section in any gas freely escape via opening 304.
The liquid meter of scheduled volume to test chamber 216 will allow this program via single pressurization Event and carry out.Therefore, the coordination of the filling of parallel multiple test chambers is simplified.
Fig. 5 shows the exemplary arrangement of the multiple test chambers 216 according to an embodiment. Single connection entrance (or port) 502 is shown as being connected to by multiple test chambers 216 The such as fluid network of testing cassete system 100.In one embodiment, single entrance 502 Receiving single port, wherein this single port is the only outside opening of fluid system.
In an example shown, because being provided solely for single entrance 502 in order to be arrived by liquid meter In multiple test chambers 216, so fluid passage includes multiple corridor diversion device 504a-c.Logical The geometry of road diverter 504a-c might be chosen such that the half of the liquid of arrival along One path flowing, and second half flows along another path.Alternatively, This geometry can be chosen in order to form dividing of any ratio between the passage of two gained Flowing liquid.Multiple test chambers 216 need not the most whole straight alignment, but can With to keep the total path length between single entrance 502 and each test chamber 216 equal Any mode is arranged.Equal path simplifies the liquid meter of the amount of will be controlled by and arrives Program in each test chamber 216.
Corridor diversion device 504a-c helps the liquid meter of equivalent to each test chamber 216 In.For example, it is possible to introduce the liquid of 80 μ L via single connection entrance 502.In succession wearing After crossing corridor diversion device 504a, 504b and 504c, the liquid of 10 μ L will be metered into each In test chamber 216.Although this example considers that each corridor diversion device 504a-c is 50/50 Fluid diverter, but need not be this situation, can be real between test chamber 216 The gained Fluid Volume of what ratio incumbent.
Passage shown in Fig. 5 is arranged and is represented closed fluid system, and it allows via single pressurization thing Part implements metering more easily.Such as, the normal pressure being applied to single connection entrance 502 can To be used in the liquid meter of scheduled volume to each test chamber 216, as before with reference to figure Described in 4A-C.
Fig. 6 A-C describes the exemplary operation of the test chamber 216 according to an embodiment. In fig. 6, test chamber 216 is filled with liquid, until the height bigger than height h.Can To apply normal pressure, so that chamber 216 is filled until this point.In one embodiment, this is System can include pressure transducer and/or actuator, to control pressure applied, and senses When liquid has arrived at threshold quantity.
In fig. 6b, apply negative pressure, and by access road 302 by liquid from test Sucking-off in chamber 216.The speed that negative pressure can be applied to so that fluid is sucked out is faster than fluid Flow into speed during test chamber 216.
In figure 6 c, liquid is sucked out, until the liquid level in test chamber 216 declines To height below h.After removing negative pressure, the liquid of scheduled volume 602 is retained in test chamber In room 216.The definite volume of scheduled volume 602 depends on the height h of opening 304, test chamber The hydraulic diameter of room 216 and the pressure applied during drawing liquid.Utilize this process, Can only by single access road 302 by the liquid meter of amount of calculation to test chamber 216 In.Such as, the amount staying liquid in the chamber can be by the surface tension on gravity and liquid The position overcoming the negative pressure being applied to passage is determined.In one example, from test chamber In room 216, the speed of sucking liquid is sufficiently fast so that be not likely to be present in test chamber 216 In reagent by access road 302 sucked back.Highly h can be for test chamber 216 Various designs are adjusted, to regulate the amount of the liquid stayed in test chamber 216.
Fig. 7 shows the embodiment of multichannel chamber 702.Two access road 704a-b are respectively It is connected to multichannel chamber 702 via opening 706a-b.Each opening edge in opening 706a-b The length multichannel chamber 702 is separately positioned on height h1And h2Place.
Multichannel chamber 702 can be used to measure the various controlled of different liquids in same chamber Level.Such as, in the case of access road 704b closes relative to air, first liquid Can be flowed in multichannel chamber 702 by access road 704a.Utilize and reference Fig. 4 A-C Described similar process, the liquid of scheduled volume can be via access road 704a and 704b phase It is metered into continuing in multichannel chamber 702.In another embodiment, utilize with reference to figure Similar process described in 6A-C, a certain amount of first and second liquid stay multichannel chamber In 702.The amount of the first liquid stayed can correspond to the height h of the first opening 706a1.So After, access road 704a closes relative to air, and second liquid passes through access road 704b simultaneously It is flowed in multichannel chamber 702.Furthermore, utilize the similar process having been described above, predetermined The second liquid of amount is stayed in multichannel chamber 702.The amount of the second liquid stayed can be corresponding Ground is corresponding to the height h of opening 706b and 706a2And h1Between difference.
Although should be appreciated that and illustrate only two access roades, but any amount of entrance Passage can realize transmitting liquid at multiple height for the length along multichannel chamber 702 Body.
Fig. 8 show according to another embodiment can be arranged in box housing 102 multiple Test chamber 216.Each test chamber 216 in multiple test chambers includes access road 302. Each access road 302 can be connected further to fluid network, such as around testing cassete 102 Fluid network.
Single pressure source (not shown) can be connected to this system so that liquid along multiple enter Each access road flowing in mouth passage 302.Therefore, it can utilize identical pressure source Fill each test chamber in multiple test chamber 216.It addition, during applying negative pressure, The fluid of same predetermined amount can be stayed in each test chamber in multiple test chamber 216. Same amount of fluid can be stayed in each test chamber 216, and with each access road 302 Between geometrical shape difference unrelated, reason is that each test chamber 216 is similarly pressurizeed. In another example, liquid can be pressurized in each passage, until being arranged on each Liquid sensing region in passage, in order to set before by liquid meter to test chamber 216 The liquid of the scheduled volume in fixed each passage.
Fig. 9 shows the multiple test chambers according to another embodiment.Replace each entrance to lead to Road 901 is connected to single chamber, and each access road 901 includes fluid diverter 902, Liquid flow is flowed at least two split channel 903a-b.Then, described at least two Split channel 903a-b can be connected at least two test chamber 904a-b.Although each entrance Passage is shown in Figure 9 for being diverted in two split channel 903a-b, but in view of this Literary composition description in the case of, those skilled in the technology concerned should be understood how by any enter Mouth passage 901 is diverted in other passage any number of.Each split channel 903a-b is permissible The opening 906a-b arranged via the length along corresponding test chamber is connected to test accordingly Chamber 904a-b.In one example, each access road 901 can be connected further to stream Volume grid.
There is provided to multiple test chambers from small number of access road be fed with helping guaranteeing each Test chamber is contained in the compound of same concentrations present in liquid.Additionally, utilize single enter Mouth passage reduces the single pressure source of connection to control to each chamber to measure multiple test chambers The complexity of fluid stream.
Figure 10 shows the analyzer 1001 according to an embodiment, and it carries out operating to perform The optical detection of the compound in testing cassete system 100.Analyzer 1001 includes optical probe 1002, temperature control component 1004, fan 1006 and include the Photoelectric Detection of object lens 1010 Device unit 1008.
Optical probe 1002 can be aligned above in passage expansion section 214, to detect respective channel The existence of liquid in chamber.Optical probe 1002 can utilize infrared ray or visible wavelength, and And include any number of detector and emitter element.It addition, collect from optical probe 1002 To data can be used to other parts of control analyzer 1001.Such as, at optical probe 1002 have been detected by after liquid existed one section of threshold time period, can send signal with Stop applying normal pressure to liquid and/or utilizing temperature control component 1004 to begin to warm up testing cassete The content of the test chamber of system 100.
Temperature control component 1004 can be arranged on survey along the base section of testing cassete system 100 Near examination chamber.Temperature control component 1004 can include heating and/or cooling down test chamber The parts of the content of room.Such as, temperature control component 1004 can be apply thermoelectricity heating or The Peltier device of cooling.In another example, temperature control component 1004 is resistance heating Device.Electric current can be through printing coil from the teeth outwards or metal band, with heating of surrounding regions. In another example, temperature control component 1004 provides forces air, so that survey is heated or cooled Examination chamber.Force air can be provided by fan 1006.In one embodiment, analyzer 1001 have temperature control component on the both sides of test chamber.Such as, a temperature controls unit Part may be used for heating, and another temperature control component may be used for cooling.
Photodetector unit 1008 can include known to those skilled in the relevant art any The fluorescence detector of type, includes but not limited to ccd array, diode and CMOS sensing Device.In one embodiment, photodetector unit 1008 provides excitation wavelength to test chamber Light, and collect the fluorescence penetrated by object lens 1010.In another embodiment, by another Source (not shown) provides excitation wavelength.The fluorescence of injection is along the base section warp of test chamber Entered region 240 by optics to penetrate from test chamber.
Figure 11 is the flow chart of the chamber fill method 1100 according to an embodiment.
At square frame 1102, the liquid of primary quantity flows along the first access road.First entrance Passage can be the such as connection entrance 502 shown in Fig. 5.
At square frame 1104, the liquid of primary quantity is divided in multiple second access road, respectively Individual second access road is connected to multiple test chamber.In one embodiment, multiple test chambers Each test chamber in room have along chamber length arrange only one opening, with In receiving in the second access road one.The length of each test chamber can be with gravitational vectors Generally parallel it is directed at.
At square frame 1106, each test chamber in multiple test chambers is filled with final quantity Liquid, the liquid of this final quantity is the most equal in each test chamber.It addition, it is every The liquid of the final quantity in individual test chamber and equal to the liquid of primary quantity.
Other action is considered as equally a part for chamber fill method 1100.Such as, Chamber fill method 1100 may include that one or more by be arranged in multiple test chamber One or more reagent in test chamber are resuspended in being metered in each test chamber Eventually in the liquid of amount.Another exemplary action includes: heat in multiple test chamber at least Content in one test chamber.This heating can pass through such as Peltier device, resistance Heating element heater and/or pressure air perform.During chamber fill method 1100, it is also possible to Detect one or more light of content at least one test chamber in multiple test chamber Learn characteristic.
Figure 12 is the flow chart of the chamber fill method 1100 according to an embodiment.
At square frame 1202, liquid flows through multiple access road.According to an embodiment, each Access road is connected to multiple test chamber.The length of each test chamber is basic with gravitational vectors On be directed at abreast.In one embodiment, each test chamber in multiple test chambers There is the only one opening of the length setting along chamber.In one example, via single Pumping source performs the flowing by multiple access roades.
At square frame 1204, each test chamber in multiple test chambers is filled with liquid, Until threshold quantity.In one example, this threshold quantity is equal to or more than along each test chamber Length arrange the height residing for opening.
At square frame 1206, by access road by the liquid each survey from multiple test chambers Examination chamber sucking-off, to leave the liquid of scheduled volume in each test chamber.The liquid of scheduled volume Can be in along below the height residing for the opening of the length setting of each test chamber Quantitative liquid.Such as, the amount staying liquid in the chamber can be by gravity and liquid Surface tension overcomes the position of the negative pressure being applied to passage to be determined.
Other action is considered as equally a part for chamber fill method 1200.Such as, Chamber fill method 1200 may include that one or more by be arranged in multiple test chamber One or more reagent in test chamber are resuspended in staying in one or more test chamber A certain amount of liquid in.If reagent is re-suspended, then perform liquid in square frame 1206 The absorption of body, the speed of absorption does not comprise reagent near the liquid being sufficient so that be drawn.Another Individual exemplary action includes: that heats at least one test chamber in multiple test chamber is interior Tolerant.This heating can be by such as Peltier device, stratie and/or pressure sky Gas performs.During chamber fill method 1200, it is also possible to detect in multiple test chamber One or more optical characteristics of content at least one test chamber.
The flow chart of the chamber fill method 1200 of another embodiment according to Figure 13.
At square frame 1302, first liquid flows through the first access road.In one embodiment, First access road is connected to first open along what the length of test chamber was arranged at the first height Mouthful.The length of test chamber is generally parallel directed at gravitational vectors.At first liquid in side While flowing through the first access road at frame 1302, the second access road coupled with test chamber Close relative to air.In one example, both the first and second access roades are when starting All lead to air.
At square frame 1304, test chamber is filled with first liquid, until first threshold amount. In one example, first threshold amount is high equal to or more than the first of the first opening of test chamber Degree.
At square frame 1306, by the first access road by first liquid from test chamber sucking-off, To leave the liquid of the first scheduled volume in test chamber.The liquid of the first scheduled volume can be with A certain amount of liquid that the first of first opening of test chamber is highly corresponding.Such as, chamber is stayed The amount of the liquid in room can be overcome by the surface tension on gravity and liquid be applied to first lead to The position of the negative pressure in road is determined.
At square frame 1308, according to an embodiment, the first access road is closed relative to air, And the second access road is opened.Can hold via the one or more valves coupled with fluid network The switching of row active channels.
At square frame 1310, second liquid flows through the second access road.In one embodiment, Second access road is connected to the second opening, and this second opening is arranged along the length of test chamber At second height bigger than first.In one example, via the single pumping source side of execution The flowing of the second liquid in the flowing of the first liquid in frame 1302 and square frame 1308.
At square frame 1312, test chamber is filled with second liquid, until Second Threshold amount.
At square frame 1314, by the second access road by second liquid from test chamber sucking-off, To leave the liquid of the second scheduled volume at test chamber.In one example, second scheduled volume Liquid be and test chamber the second opening second height and the first height of the first opening between A certain amount of liquid corresponding to difference.In another example, stay the amount of liquid in the chamber The portion of the negative pressure being applied to second channel can be overcome by the surface tension on gravity and liquid Position is determined.
Being similar to chamber fill method 1200, other action is considered chamber fill method The part of 1300.Such as, chamber fill method 1300 may include that and will be arranged on test The first of the scheduled volume that one or more reagent in chamber are resuspended in staying in test chamber With in second liquid.Alternatively, one or more reagent described can hang again Float in the only first liquid staying in test chamber.In one example, in fact it could happen that Any reagent in test chamber will not be sucked back into first respectively in square frame 1306 and 1314 Or second in access road.Chamber fill method 1300 can also include: heating test chamber Content and/or one or more optical characteristics of content of detection test chamber, such as pin before To described in chamber fill method 1200.Although only illustrating in chamber fill method 1300 Two access roades, but it is to be understood that chamber fill method 1300 can expand to include Lead to any number of access road in one or more chamber.
The flow chart of the chamber fill method 1400 of another embodiment according to Figure 14.
At square frame 1402, according to an embodiment, it is each logical that liquid flows through in multiple passage Road, until arranging liquid sensing region in each channel.Liquid can be pressurized to along Each channel flow, and can be individually or simultaneously along each channel flow.According to one Individual embodiment, make liquid flowing until sensing region set in multiple passage each passage in The liquid of scheduled volume.
At square frame 1404, according to an embodiment, pre-in each passage in multiple passages Quantitative liquid is flowed in the corresponding chambers coupled with each passage.Each passage can be independent Ground or be simultaneously pressurized to the chamber forcing the liquid of scheduled volume to be downwardly into each correspondence.
Method 1400 can also include: will be arranged in the one or more passages in multiple passage One or more reagent Eddy diffusions.This reagent can be arranged on pre-with what each passage coupled In first mixing chamber.
The preceding description of specific embodiment will reflect the general characteristic of the present invention completely so that not In the case of departing from the general concept of the present invention, it is in those skilled in the art's model by application Enclosing interior knowledge, such specific embodiment easily can be revised and/or be modified to by other people For various application.Therefore, based on teaching herein and guiding, at such amendment and change In the equivalent and scope of embodiment disclosed by the invention.Should be appreciated that term herein Or the purpose that illustrates that of term rather than in order to limit, thus the term of this specification Should be understood according to teaching and guiding by those skilled in the art with term.
Below describing embodiments of the invention by means of formation function block, these blocks illustrate The enforcement of specific function and relation thereof.The border of these formation function blocks is in this article in order to say Bright convenience and optionally limit.Selective border can be limited, as long as can be suitably Perform specific function and relation thereof.
Summary of the invention and summary part can list or many expected of the present invention by inventor Individual but be not all, of exemplary embodiment, therefore it is not used to limit by any way the present invention With appended claim.
The range of the present invention and scope should not limited by any of the above-described exemplary embodiments, but Should be defined only according to claims below and equivalents thereof.

Claims (72)

1. a fluid test system, comprising:
Multiple test chambers, each test chamber is characterised by that length and hydraulic diameter, each test chamber in wherein said multiple test chambers have the only one opening of the length setting along corresponding test chamber；
First access road, described first access road is configured to the liquid making primary quantity and flows from ingress port, and wherein said ingress port is the described only outside opening of fluid test system；And
Multiple divided fluid stream elements, the plurality of divided fluid stream element is configured to be diverted in multiple second access road along the original liquid of described first access road flowing, only one the second access road that each test chamber in wherein said multiple test chambers is connected in the plurality of second access road via its corresponding opening.
Fluid test system the most according to claim 1, the length of the most each test chamber is configured to be directed at substantially in parallel with gravitational vectors.
Fluid test system the most according to claim 1, it also includes the multiple liquid sensing regions arranged along the plurality of second access road.
Fluid test system the most according to claim 3, each liquid sensing region in wherein said multiple liquid sensing regions is configured to monitor the existence of liquid in corresponding test chamber.
Fluid test system the most according to claim 3, each liquid sensing region in wherein said multiple liquid sensing regions is configured in the liquid meter of scheduled volume to corresponding test chamber.
Fluid test system the most according to claim 1, at least one test chamber in wherein said multiple test chambers includes one or more reagent.
Fluid test system the most according to claim 6, one or more reagent wherein said include the lyophilizing globule being arranged in described test chamber.
Fluid test system the most according to claim 1, it also includes being pre-mixed chamber along what each second access road in the plurality of second access road was arranged.
Fluid test system the most according to claim 8, wherein said multiple chambers that are pre-mixed comprise one or more reagent.
Fluid test system the most according to claim 1, the base wall of each test chamber in wherein said multiple test chambers has the geometry of bending.
11. fluid test systems according to claim 1, the base wall of each test chamber in wherein said multiple test chambers is transparent, to allow to be optically inspected.
12. fluid test systems according to claim 1, the most single pump is configured to pass the first access road so that the liquid of primary quantity, and each test chamber in the plurality of test chamber is filled with the liquid of equal quantities.
13. fluid test systems according to claim 1, one or more walls of plurality of chamber contact with the housing of thermal control.
14. fluid test systems according to claim 13, wherein said one or more walls are the polymeric films that thickness is less than 100 microns.
15. fluid test systems according to claim 13, the thermal conductivity of wherein said one or more walls is more than 1W/mK.
16. fluid test systems according to claim 13, the housing of wherein said thermal control includes Peltier device.
17. fluid test systems according to claim 13, the housing of wherein said thermal control includes stratie.
18. fluid test systems according to claim 13, the housing of wherein said thermal control heats via pressure air.
19. 1 kinds of chamber fill methods, comprising:
Make the liquid first access road flowing along single port fluid test system of primary quantity；
The liquid of described primary quantity is diverted to multiple second access road from described first access road, each second access road is connected to multiple test chamber, and each test chamber in wherein said multiple test chambers has the only one opening of the length setting along chamber；And
Each test chamber in the plurality of test chamber is filled with the liquid of final quantity, and the final quantity in each test chamber is substantially identical, and the liquid of the final quantity of each test chamber and equal to the liquid of described primary quantity.
20. methods according to claim 19, the length of the most each test chamber is configured to be directed at substantially in parallel with gravitational vectors.
21. methods according to claim 19, it also includes: be resuspended in the liquid of described final quantity by one or more reagent being arranged in the one or more test chambers in the plurality of test chamber.
22. methods according to claim 19, it also includes: be resuspended in being arranged on multiple one or more one or more reagent being pre-mixed in chamber being pre-mixed in chamber in the liquid of described final quantity.
23. methods according to claim 19, it also includes heating steps: the content at least one test chamber heating in the plurality of test chamber.
24. methods according to claim 23, wherein said heating steps includes utilizing Peltier device to heat.
25. methods according to claim 23, wherein said heating steps includes utilizing stratie to heat.
26. methods according to claim 23, wherein said heating steps includes utilizing forces air to heat.
27. methods according to claim 19, it also includes one or more optical characteristics of the content at least one test chamber detecting in the plurality of test chamber.
28. methods according to claim 19, wherein via single pumping source execution flows, shunt and fill.
29. 1 kinds of fluid test systems, comprising:
Multiple test chambers, each test chamber is characterised by length and hydraulic diameter, the length of the most each test chamber is configured to be directed at substantially in parallel with gravitational vectors, and each test chamber in wherein said multiple test chamber has the only one opening of the length setting along corresponding test chamber；
Multiple access roades, the only one access road that each test chamber in wherein said multiple test chambers is connected in the plurality of access road via its corresponding opening；And
Fluid network, described fluid network is configured to be connected to the plurality of access road other chamber one or more.
30. fluid test systems according to claim 29, it also includes the multiple liquid sensing regions arranged along the plurality of access road.
31. fluid test systems according to claim 30, each liquid sensing region in wherein said multiple liquid sensing regions is configured to monitor the existence of liquid in corresponding test chamber.
32. fluid test systems according to claim 30, each liquid sensing region in wherein said multiple liquid sensing regions is configured in the liquid meter of scheduled volume to corresponding test chamber.
33. fluid test systems according to claim 29, wherein remaining liquid by corresponding access road after the only one opening of the length along corresponding test chamber is sucked out, the amount of the liquid in corresponding chambers is stayed in the position control of described only one opening.
34. fluid test systems according to claim 29, the width of wherein said only one opening is more than the width of corresponding access road.
35. fluid test systems according to claim 29, at least one test chamber in wherein said multiple test chambers includes one or more reagent.
36. fluid test systems according to claim 35, one or more reagent wherein said include the lyophilizing globule being arranged in described test chamber.
37. fluid test systems according to claim 29, it also includes being pre-mixed chamber along what each second access road in the plurality of second access road was arranged.
38. according to the fluid test system described in claim 37, and the wherein said chamber that is pre-mixed comprises one or more reagent.
39. fluid test systems according to claim 29, the base wall of each test chamber in wherein said multiple test chambers has the geometry of bending.
40. fluid test systems according to claim 29, the base wall of each test chamber in wherein said multiple test chambers is transparent, to allow to be optically inspected.
41. fluid test systems according to claim 29, the most single pump is used for making liquid pass the plurality of access road.
42. fluid test systems according to claim 29, one or more walls of plurality of chamber contact with the housing of thermal control.
43. fluid test systems according to claim 42, wherein said one or more walls are the polymeric films that thickness is less than 100 microns.
44. fluid test systems according to claim 42, the thermal conductivity of wherein said one or more walls is more than 1W/mK.
45. fluid test systems according to claim 42, the housing of wherein said thermal control includes Peltier device.
46. fluid test systems according to claim 42, the housing of wherein said thermal control includes stratie.
47. fluid test systems according to claim 42, the housing of wherein said thermal control heats via pressure air.
48. fluid test systems according to claim 29, at least one access road in wherein said multiple access roades is connected to more than one opening.
49. 1 kinds of chamber fill methods, comprising:
Flow liquid through multiple access road, each access road is connected to multiple test chamber, the length of the most each test chamber is configured to be directed at substantially in parallel with gravitational vectors, and each test chamber in wherein said multiple test chamber has the only one opening of the length setting along chamber；
Each test chamber in the plurality of test chamber is filled with liquid, until threshold quantity；
By described access road by the liquid each test chamber sucking-off from the plurality of test chamber, the most this sucking-off leaves the liquid of scheduled volume in each test chamber.
50. methods according to claim 49, it also includes: be resuspended in a certain amount of liquid staying in the one or more test chamber by one or more reagent being arranged in the one or more test chambers in the plurality of test chamber.
51. methods according to claim 50, the liquid not comprising reagent is drawn in wherein said sucking-off.
52. methods according to claim 49, wherein fill and include filling each test chamber until threshold quantity until a height, and described height is equal to or more than the height residing for the opening along the length setting of each test chamber.
53. methods according to claim 52, wherein stay the liquid of scheduled volume to include leaving a certain amount of liquid being in along below the height residing for the opening of the length setting of each test chamber.
54. methods according to claim 49, it also includes heating steps: the content at least one test chamber heating in the plurality of test chamber.
55. methods according to claim 54, wherein said heating steps includes utilizing Peltier device to heat.
56. methods according to claim 54, wherein said heating steps includes utilizing stratie to heat.
57. methods according to claim 54, wherein said heating steps includes utilizing forces air to heat.
58. methods according to claim 49, it also includes one or more optical characteristics of the content at least one test chamber detecting in the plurality of test chamber.
59. methods according to claim 49, wherein via single pumping source execution flows.
60. 1 kinds of chamber fill methods, comprising:
Making first liquid flow through the first access road, described first access road is connected to be arranged on the first opening at the first height along the length of test chamber, and the length of wherein said test chamber is configured to be directed at substantially in parallel with gravitational vectors；
Described test chamber is filled with first liquid, until first threshold amount；
By described first access road by described first liquid from described test chamber sucking-off, wherein said sucking-off leaves the first liquid of the first scheduled volume in described test chamber；
Making second liquid flow through the second access road, described second access road is connected to be arranged on the second opening at the second height along the length of described test chamber, and wherein said second height is more than described first height；
Described test chamber is filled with described second liquid, until Second Threshold amount；
By described second access road by described second liquid from described test chamber sucking-off, wherein said sucking-off leaves the second liquid of the second scheduled volume in described test chamber.
61. methods according to claim 60, wherein stay the first liquid of the first scheduled volume to include leaving a certain amount of first liquid the most corresponding with the first of described first opening.
62. methods according to claim 61, wherein stay the second liquid of the second scheduled volume to include leaving a certain amount of second liquid corresponding with the difference between the second of described second opening the height and the first height of described first opening.
63. methods according to claim 60, it also includes: be resuspended in staying by one or more reagent being arranged in described test chamber in first liquid and the second liquid of the scheduled volume in described test chamber.
64. methods according to claim 60, it also includes: be resuspended in staying in the first liquid of the only scheduled volume in described test chamber by one or more reagent being arranged in described test chamber.
65. methods according to claim 64, wherein sucking-off first liquid or second liquid draw the liquid not comprising any reagent.
66. methods according to claim 60, it also includes heating steps: heat the content in described test chamber.
67. methods according to claim 66, wherein said heating steps includes utilizing Peltier device to heat.
68. methods according to claim 66, wherein said heating steps includes utilizing stratie to heat.
69. methods according to claim 66, wherein said heating steps includes utilizing forces air to heat.
70. methods according to claim 60, its one or more optical characteristics also including detecting the content in described test chamber.
71. methods according to claim 60, wherein perform first liquid and the flowing of second liquid via single pumping source.
72. methods according to claim 60, it also includes: after sucking-off first liquid, closed relative to air by described first access road.
CN201380034569.3A 2012-05-09 2013-05-09 Multiple reaction chambers in testing cassete Active CN104582849B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610103877.2A CN105642378B (en) 2012-05-09 2013-05-09 Multiple reaction chambers in testing cassete

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261644858P 2012-05-09 2012-05-09
US61/644,858 2012-05-09
US13/837,007 US9063121B2 (en) 2012-05-09 2013-03-15 Plurality of reaction chambers in a test cartridge
US13/837,007 2013-03-15
PCT/EP2013/059692 WO2013167716A2 (en) 2012-05-09 2013-05-09 Plurality of reaction chambers in a test cartridge

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201610103877.2A Division CN105642378B (en) 2012-05-09 2013-05-09 Multiple reaction chambers in testing cassete

Publications (2)

Publication Number Publication Date
CN104582849A CN104582849A (en) 2015-04-29
CN104582849B true CN104582849B (en) 2016-08-17

Family

ID=49548890

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201610103877.2A Active CN105642378B (en) 2012-05-09 2013-05-09 Multiple reaction chambers in testing cassete
CN201380034569.3A Active CN104582849B (en) 2012-05-09 2013-05-09 Multiple reaction chambers in testing cassete

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201610103877.2A Active CN105642378B (en) 2012-05-09 2013-05-09 Multiple reaction chambers in testing cassete

Country Status (12)

Country Link
US (6) US9063121B2 (en)
EP (1) EP2846911B1 (en)
JP (2) JP6375291B2 (en)
KR (4) KR20190128257A (en)
CN (2) CN105642378B (en)
AU (2) AU2013258002B2 (en)
BR (1) BR112014027737B1 (en)
CA (1) CA2871856C (en)
ES (1) ES2834461T3 (en)
RU (1) RU2644476C2 (en)
WO (1) WO2013167716A2 (en)
ZA (2) ZA201500089B (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8448499B2 (en) 2008-12-23 2013-05-28 C A Casyso Ag Cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, a corresponding measuring system, and a corresponding method
JP6335802B2 (en) * 2012-03-12 2018-05-30 バイオサーフィット、 ソシエダッド アノニマ Liquid sample imaging apparatus and method
US9063121B2 (en) 2012-05-09 2015-06-23 Stat-Diagnostica & Innovation, S.L. Plurality of reaction chambers in a test cartridge
US10539579B2 (en) 2014-09-29 2020-01-21 C A Casyso Gmbh Blood testing system and method
US10274492B2 (en) * 2015-04-10 2019-04-30 The Curators Of The University Of Missouri High sensitivity impedance sensor
CN108027382B (en) * 2015-07-07 2021-12-24 华盛顿大学 Systems, methods, and apparatus for sample self-digitization
US10775370B2 (en) * 2015-07-17 2020-09-15 Stat-Diagnostica & Innovation, S.L. Fluidic system for performing assays
JP7073328B2 (en) * 2016-03-14 2022-05-23 ヘリックスバインド・インコーポレイテッド Methods associated with integrated fluid devices
CN109475869B (en) * 2016-07-18 2021-07-30 西门子医疗保健诊断公司 Liquid assay reagent dispensing apparatus and related assay kits and methods of use
USD840049S1 (en) * 2016-11-11 2019-02-05 Robert Bosch Gmbh Cartridge for an analysis unit
CN109991035B (en) * 2017-12-29 2021-12-24 台达电子工业股份有限公司 Micro-sampling device
US10046322B1 (en) * 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
KR102105558B1 (en) * 2018-03-23 2020-04-28 (주)바이오니아 Analysis Plate For Polymerase Chain Reaction
US20220205991A1 (en) * 2019-02-22 2022-06-30 Retala Leasing, LLC Controlled Generation of Measurable Signals and Uses Thereof
KR102426788B1 (en) * 2019-06-30 2022-07-29 주식회사 진시스템 Pcr pretreatmet method and multiplex pcr chip thereof
US10820847B1 (en) 2019-08-15 2020-11-03 Talis Biomedical Corporation Diagnostic system
USD975312S1 (en) 2020-02-14 2023-01-10 Beckman Coulter, Inc. Reagent cartridge
CN112570052A (en) * 2020-12-07 2021-03-30 厦门大学 Micro-fluidic device containing probe array and micro-fluidic chip with side channels
CN112945842A (en) * 2021-01-27 2021-06-11 中国科学院金属研究所 Pipeline microbial corrosion monitoring and early warning system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004028692A3 (en) * 2002-09-23 2004-07-29 Prisma Diagnostika Gmbh Supporting element for conducting diagnostic tests
CN101194155A (en) * 2005-04-09 2008-06-04 贝林格尔英格海姆米克罗帕茨有限责任公司 Device and method for analyzing a sample liquid
CN101883985A (en) * 2008-02-05 2010-11-10 松下电器产业株式会社 Analyzing device, and analyzing apparatus and analyzing method using the device

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5380437A (en) * 1993-02-02 1995-01-10 Biomedical Research And Development Laboratories, Inc. Multifunctional filtration apparatus
US7244622B2 (en) 1996-04-03 2007-07-17 Applera Corporation Device and method for multiple analyte detection
JP4912517B2 (en) 1996-04-03 2012-04-11 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー Devices and methods for detection of multiple analytes
AU6343398A (en) 1997-02-28 1998-09-18 Cepheid Heat exchanging, optically interrogated chemical reaction assembly
US5786182A (en) 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
WO1999033559A1 (en) 1997-12-24 1999-07-08 Cepheid Integrated fluid manipulation cartridge
AU2003200701B2 (en) 1997-12-24 2005-12-08 Cepheid Integrated fluid manipulation cartridge
BR9909249B1 (en) * 1998-03-11 2009-12-01 sample holder.
US20020068358A1 (en) 1998-04-28 2002-06-06 Campbell Michael J. In vitro embryo culture device
US6830934B1 (en) 1999-06-15 2004-12-14 Lifescan, Inc. Microdroplet dispensing for a medical diagnostic device
FR2782729B1 (en) * 1998-09-01 2002-10-25 Bio Merieux ENUMERATION AND CHARACTERIZATION MAP OF MICROORGANISMS
US6436722B1 (en) * 2000-04-18 2002-08-20 Idexx Laboratories, Inc. Device and method for integrated diagnostics with multiple independent flow paths
US6514750B2 (en) 2001-07-03 2003-02-04 Pe Corporation (Ny) PCR sample handling device
US7157049B2 (en) 2001-11-20 2007-01-02 Nagaoka & Co., Ltd. Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
US7244961B2 (en) * 2002-08-02 2007-07-17 Silicon Valley Scientific Integrated system with modular microfluidic components
US7338637B2 (en) * 2003-01-31 2008-03-04 Hewlett-Packard Development Company, L.P. Microfluidic device with thin-film electronic devices
JPWO2005024436A1 (en) * 2003-09-02 2007-11-08 日本電気株式会社 Customizable chip and manufacturing method thereof
US8133703B2 (en) * 2004-10-27 2012-03-13 Ceoheid Closed-system multi-stage nucleic acid amplification reactions
JP4458253B2 (en) * 2004-10-28 2010-04-28 有限会社バイオデバイステクノロジー Microchip for specimen sample
WO2006080140A1 (en) * 2005-01-28 2006-08-03 Matsushita Electric Industrial Co., Ltd. Blood treatment apparatus and blood supply method
WO2006108559A2 (en) * 2005-04-09 2006-10-19 Boehringer Ingelheim Microparts Gmbh Device and method for analyzing a sample liquid
CN101351542A (en) * 2005-07-15 2009-01-21 阿普尔拉公司 Fluid processing device and method
US8075851B2 (en) 2005-09-29 2011-12-13 Siemens Medical Solutions Usa, Inc. Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography
US20070201933A1 (en) 2006-02-24 2007-08-30 Park Namjeon Feeding system for image forming machine
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
FI20065269A0 (en) 2006-04-26 2006-04-26 Nokia Corp Spectrum utilization in a radio system
JP4556194B2 (en) 2008-02-01 2010-10-06 セイコーエプソン株式会社 Biological sample reaction method
US20090269800A1 (en) * 2008-04-29 2009-10-29 Todd Covey Device and method for processing cell samples
NZ590411A (en) * 2008-07-11 2013-07-26 Universal Biosensors Pty Ltd Enhanced immunoassay sensor
US9063121B2 (en) 2012-05-09 2015-06-23 Stat-Diagnostica & Innovation, S.L. Plurality of reaction chambers in a test cartridge

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004028692A3 (en) * 2002-09-23 2004-07-29 Prisma Diagnostika Gmbh Supporting element for conducting diagnostic tests
CN101194155A (en) * 2005-04-09 2008-06-04 贝林格尔英格海姆米克罗帕茨有限责任公司 Device and method for analyzing a sample liquid
CN101883985A (en) * 2008-02-05 2010-11-10 松下电器产业株式会社 Analyzing device, and analyzing apparatus and analyzing method using the device

Also Published As

Publication number Publication date
CN104582849A (en) 2015-04-29
JP6585119B2 (en) 2019-10-02
AU2016208331B2 (en) 2018-08-02
ZA201500089B (en) 2017-07-26
US20150284773A1 (en) 2015-10-08
EP2846911B1 (en) 2020-09-30
KR102148730B1 (en) 2020-08-28
KR20190062620A (en) 2019-06-05
ZA201600473B (en) 2017-04-26
KR20190128257A (en) 2019-11-15
US10159972B2 (en) 2018-12-25
US10092900B2 (en) 2018-10-09
JP6375291B2 (en) 2018-08-15
US20130302809A1 (en) 2013-11-14
KR102127231B1 (en) 2020-06-29
AU2013258002A1 (en) 2014-11-13
EP2846911A2 (en) 2015-03-18
JP2017142271A (en) 2017-08-17
KR102366452B1 (en) 2022-02-23
AU2016208331A1 (en) 2016-08-11
BR112014027737B1 (en) 2021-05-11
US10099216B2 (en) 2018-10-16
AU2013258002B2 (en) 2016-08-11
ES2834461T3 (en) 2021-06-17
US20150284771A1 (en) 2015-10-08
US20150283542A1 (en) 2015-10-08
US20150284772A1 (en) 2015-10-08
RU2014144122A (en) 2016-07-10
BR112014027737A2 (en) 2017-06-27
CA2871856A1 (en) 2013-11-14
CN105642378A (en) 2016-06-08
WO2013167716A2 (en) 2013-11-14
US20150284774A1 (en) 2015-10-08
RU2644476C2 (en) 2018-02-12
KR20150016313A (en) 2015-02-11
CA2871856C (en) 2020-12-29
US9968928B2 (en) 2018-05-15
JP2015516085A (en) 2015-06-04
US9855553B2 (en) 2018-01-02
WO2013167716A3 (en) 2014-01-09
KR20200143742A (en) 2020-12-24
US9063121B2 (en) 2015-06-23
CN105642378B (en) 2018-06-08

Similar Documents

Publication Publication Date Title
CN104582849B (en) Multiple reaction chambers in testing cassete
CN104411406B (en) Testing cassete with integrated delivery module
CN104838268B (en) Microfluid LAL reactive materials method of testing and equipment
CN104136123B (en) Microfluidic reactor system
US20140295490A1 (en) Fetal red blood cell detection
KR20210065460A (en) Micro-chip for analyzing fluids and method for amplification of genes using the same
JP2009133668A (en) Inspection apparatus

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant