CN103583606B - Root knot nematode control agent and its preparation method and application - Google Patents
Root knot nematode control agent and its preparation method and application Download PDFInfo
- Publication number
- CN103583606B CN103583606B CN201310601194.6A CN201310601194A CN103583606B CN 103583606 B CN103583606 B CN 103583606B CN 201310601194 A CN201310601194 A CN 201310601194A CN 103583606 B CN103583606 B CN 103583606B
- Authority
- CN
- China
- Prior art keywords
- weight portion
- knot nematode
- root
- control agent
- root knot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to root knot nematode control agent and its preparation method and application, belong to agriculture field.The technical problem to be solved in the present invention is, provides a kind of plant resource root knot nematode control agent and its preparation method and application.Root knot nematode control agent of the present invention, its active component is prepared from by the Chinese herbal medicine raw material of following weight portion: the castor bean of the asiatic moonseed of the Sophora alopecuroide of the peganum harmala L of 1 ~ 8 weight portion, the ginkgo leaf of 0 ~ 8 weight portion, 1 ~ 10 weight portion, the root of subprostrate sophora of 0 ~ 5 weight portion, 0 ~ 5 weight portion, the kuh-seng of 0 ~ 10 weight portion, 0 ~ 8 weight portion, the Tong fiber crops of 1 ~ 10 weight portion, the garland chrysanthemum of 0 ~ 8 weight portion, the datura of 0 ~ 5 weight portion.Meanwhile, the invention also discloses the preparation method and application of root knot nematode control agent.Root-knot nematode composition of the present invention has the advantage of safety non-toxic, effectively can prevent simultaneously, suppresses and kill root-knot nematode.
Description
Technical field
The present invention relates to root knot nematode control agent and its preparation method and application, belong to agriculture field.
Background technology
Root knot nematode disease is that main plant nematode is one of sick, the multiple Vegetables such as main harm cucumber, balsam pear, tomato, eggplant, Kidney bean, celery, spinach.Along with the adjustment of crop mix; the rapid expansion of vegetable protecting field area under cultivation; root-knot nematode is pathogenetic increases the weight of year by year; production loss reaches 30% ~ 50%; ruin seedling heavily the event of planting happen occasionally; in addition, root-knot nematode harm has also increased the weight of the generation of the native transmissibility fungal disease such as fusarium wilt, root rot and part bacteriosis, has become a large obstacle of current Vegetable produce.
Chemical control is still the topmost prophylactico-therapeutic measures of plant nematode disease, but the use cost of the traditional chemical nematode killing agents such as such as chloropicrin, Celfume, carbofuran, Nemacur, Methomyl is high, and Environmental compatibility is poor.The biological control method that application has operability controls the common recognition that root-knot nematode is Chinese scholars, studies, develops, applies the trend that new and effective plant resource GR is root knot nematode control development.Many R&D institutions are had to carry out the research of this respect at present, but all do not obtain the medicament of desirable preventive effect, therefore work out and utilize plant seed or leaf, cane, obtain efficient, safe plant resource GR through extracting, and be applicable to organic farming, the production of green agriculture is problem demanding prompt solution.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of plant resource root knot nematode control agent and its preparation method and application.
Root knot nematode control agent of the present invention, its active component is prepared from by the raw material of following weight portion: the castor bean of the asiatic moonseed of the Sophora alopecuroide of the peganum harmala L of 1 ~ 8 weight portion, the ginkgo leaf of 0 ~ 8 weight portion, 1 ~ 10 weight portion, the root of subprostrate sophora of 0 ~ 5 weight portion, 0 ~ 5 weight portion, the kuh-seng of 0 ~ 10 weight portion, 0 ~ 8 weight portion, the Tong fiber crops of 1 ~ 10 weight portion, the garland chrysanthemum of 0 ~ 8 weight portion, the datura of 0 ~ 5 weight portion.
Further, preferably, the active component of root knot nematode control agent of the present invention is prepared from by the raw material of following weight portion:
The castor bean of the asiatic moonseed of the Sophora alopecuroide of the peganum harmala L of 1 ~ 8 weight portion, the ginkgo leaf of 0.5 ~ 8 weight portion, 1 ~ 10 weight portion, the root of subprostrate sophora of 0 ~ 5 weight portion, 0 ~ 5 weight portion, the kuh-seng of 0 ~ 10 weight portion, 0 ~ 8 weight portion, the Tong fiber crops of 1 ~ 10 weight portion, the garland chrysanthemum of 3 ~ 8 weight portions, the datura of 1 ~ 5 weight portion;
Or 1 ~ 5 peganum harmala L of weight portion, the Sophora alopecuroide of 1 ~ 6 weight portion, the Tong fiber crops of 1 ~ 8 weight portion, the ginkgo leaf of 1 ~ 7 weight portion, the kuh-seng of 1 ~ 5 weight portion, the castor bean of 1 ~ 8 weight portion;
Or 1 ~ 5 peganum harmala L of weight portion, the Sophora alopecuroide of 1 ~ 5 weight portion, the piemarker of 1 ~ 5 weight portion, the castor bean of 0.5 ~ 6 weight portion and 1 ~ 6 weight portion ginkgo leaf;
Or 2 ~ 5 peganum harmala Ls of weight portion, the Tong fiber crops of 2 ~ 5 weight portions, the Sophora alopecuroide of 2 ~ 5 weight portions and the castor bean of 2 ~ 5 weight portions.
Wherein, above-mentioned raw materials is Chinese herbal medicine raw material, said herbal medicine plant material directly can be pulverized or becomes thoroughly decomposed by described active component, fermenting is prepared from, in order to improve the effect of root knot nematode control agent of the present invention, preferably, its active component to be got through ethanolic solution or water extraction by said herbal medicine plant material and is prepared from.
Further, in order to improve the activity of root knot nematode control agent, in addition to the active ingredient (s also preferably containing auxiliary material in described root knot nematode control agent, described auxiliary material can adopt the conventional auxiliary material in composition, such as adopts at least one in dispersant, wetting agent, bleeding agent, synergist; Preferably, described bleeding agent is at least one in fatty alcohol polyethenoxy ether class surfactant, organosilicon bleeding agent, Organic fluoride bleeding agent; Described dispersant is at least one in lignosulfonates, sldium lauryl sulfate, phosphate, alkylphenol-polyethenoxy base phosphate; Described wetting agent is at least one in alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester; Described synergist is organosilicon.Root knot nematode control agent of the present invention can be prepared into the common dosage forms such as aqua, pulvis or granule.
Further, in described pulvis and granule, also comprise carrier, the preferred maltose of described carrier, soluble starch or fertilizer element; Wherein, at least one in described fertilizer element preferably sulfuric acid potassium, ammonium sulfate, MAP, diammonium phosphate, magnesium sulfate, potassium dihydrogen phosphate or organic matter; Organic to become thoroughly decomposed at least one that thing, careless carbon, ight soil becomes thoroughly decomposed in thing etc. for agricultural crop straw.
Wherein, described root knot nematode control agent can adopt conventional method to prepare, such as can adopt and add auxiliary material and carrier in active component, stir, dry, pulverize to obtain pulvis, also can add in active component after filler drying makes powder prepare again, mix, dry, pulverize to obtain powder product.The preparation method of granule is: active component is mixed rear granulation, drying with filler, dispersant, wetting agent, synergist etc., sieve (1 ~ 4.75um), obtains granule.
Further, in order to improve the effect of root knot nematode control agent, root knot nematode control agent of the present invention preferably adopts following three kinds of methods preparation and obtains:
Method (one):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverizes;
B, immersion: the Chinese herbal medicine raw material after the pulverizing of a step is added in solvent and soaks, obtain immersion mixture, soak time preferably 12 ~ 36 hours; Wherein, described solvent is ethanol water, preferred 1:20 ~ 50 of mass ratio of described Chinese herbal medicine raw material and solvent, the mass ratio of water and ethanol preferably 0.25 ~ 4:1 in solvent;
C, extraction: ultrasonic lixiviate 30 ~ 60min under b step gained immersion mixture is preferable over 20 ~ 40 DEG C of conditions, obtains extract;
D, preparation: extract is made aqua, pulvis or granule.
Wherein, in order to improve the production efficiency of root knot nematode control agent, reduce production cost, soak time more preferably 18 ~ 30 hours, the mass ratio of Chinese herbal medicine raw material and solvent preferably more preferably 1:30 ~ 40, the mass ratio of water and ethanol preferred 0.5 ~ 1:1 further in solvent.
Wherein, for the ease of storage and transport, extract can be concentrated, obtain concentrate or medicinal extract, and then be prepared into various formulation; Cycles of concentration can according to needs of production from Row sum-equal matrix.
Method (two):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverizes;
B, preparation: pulvis made by the raw material after a step being pulverized.
Method (three):
A, fermentation maturity: by Chinese herbal medicine raw material hybrid concurrency ferment or become thoroughly decomposed;
B, preparation: the raw material after becoming thoroughly decomposed by fermentation is made pulvis or granule;
Wherein, active in order to improve root knot nematode control agent of the present invention, preferably, ferment through row again after Chinese herbal medicine raw material is pulverized or become thoroughly decomposed.
Further, the invention also discloses the application of root knot nematode control agent in control plant root-knot nematode.In order to improve the effect of root knot nematode control agent, the use age is preferably seedling stage of plant; Root knot nematode control agent of the present invention can be used by common method, as poured into a mould, spraying, spray, dusting, distribution, its amount of application with weather condition or crop condition change.General, adopt the methods such as cast, spraying, when using by root knot nematode control agent thin up of the present invention to suitable concentration, concentration can regulate according to needs of production, amount of application preferably 2 ~ 8L/ mu/time.Preferably aqua is adopted and be sprayed on plant blade face, being more preferably when plant premorbid or their early stage, by doubly spraying the soil around pouring crop root after aqua dilution, after being preferable over 10 ~ 15 days, carrying out secondary dispenser, the preferred continuous administration of one season crop 2 ~ 3 times, amount of application is 3 ~ 8L/ mu.For pulvis or granule except rear pouring can be watered, sprays by above-mentioned extension rate, rewater after also can spreading fertilizer over the fields or mix soil, the place making active ingredient can be distributed to crop root as much as possible to reach.Also can spread fertilizer over the fields the soil of crop, scatter, mix soil before and after crops be transplanted, make worm's ovum or larva suppressed or kill, amount of application is also by 2 ~ 8 kgs/acre times, use by 2 ~ 4 kgs/acre times at Seedling Stage, with the occurring degree of root-knot nematode and insect density determination consumption 4-8 kg/acre after 10 ~ 15 days.
Root knot nematode control agent of the present invention can be widely used in the plant that can be subject to preventing and treating root-knot nematode disease, namely root knot nematode control agent of the present invention may be used for prevention plant root-knot nematode evil, or for being subject to the plant of root-knot nematode evil, the common plant being subject to root-knot nematode evil has water spinach, cucumber, tomato, carrot, Chinese cabbage, lettuce, eggplant, custard squash etc.
Beneficial effect of the present invention:
1, root knot nematode control agent use safety of the present invention, easily degrade, to human body and environmentally friendly, also can not cause the residual of hormone, can not safety problem be brought.
2, root knot nematode control agent of the present invention has good effect to control root-knot nematode, higher than the biological composition of routine, is not less than Chemical composition that.
3, root knot nematode control agent of the present invention obviously can also improve the nutrition condition of crop simultaneously, promotes growing of crop, improves its immunity.
4, root knot nematode control agent of the present invention contain enrich polysaccharide, amino acid, etc. organic matter, the organic matter needed for microorganism is provided, optimize microbial environment, improvement and fertilizing soil.
5, in the present invention, root knot nematode control agent is with low cost, and preparation method is simple, without the need to dropping into large-scale special equipment.
Embodiment
Root knot nematode control agent of the present invention, its active component is prepared from by the raw material of following weight portion: the castor bean of the asiatic moonseed of the Sophora alopecuroide of the peganum harmala L of 1 ~ 8 weight portion, the ginkgo leaf of 0 ~ 8 weight portion, 1 ~ 10 weight portion, the root of subprostrate sophora of 0 ~ 5 weight portion, 0 ~ 5 weight portion, the kuh-seng of 0 ~ 10 weight portion, 0 ~ 8 weight portion, the Tong fiber crops of 1 ~ 10 weight portion, the garland chrysanthemum of 0 ~ 8 weight portion, the datura of 0 ~ 5 weight portion.
Further, preferably, the active component of root knot nematode control agent of the present invention is prepared from by the raw material of following weight portion:
The castor bean of the asiatic moonseed of the Sophora alopecuroide of the peganum harmala L of 1 ~ 8 weight portion, the ginkgo leaf of 0.5 ~ 8 weight portion, 1 ~ 10 weight portion, the root of subprostrate sophora of 0 ~ 5 weight portion, 0 ~ 5 weight portion, the kuh-seng of 0 ~ 10 weight portion, 0 ~ 8 weight portion, the Tong fiber crops of 1 ~ 10 weight portion, the garland chrysanthemum of 3 ~ 8 weight portions, the datura of 1 ~ 5 weight portion;
Or 1 ~ 5 peganum harmala L of weight portion, the Sophora alopecuroide of 1 ~ 6 weight portion, the Tong fiber crops of 1 ~ 8 weight portion, the ginkgo leaf of 1 ~ 7 weight portion, the kuh-seng of 1 ~ 5 weight portion, the castor bean of 1 ~ 8 weight portion;
Or 1 ~ 5 peganum harmala L of weight portion, the Sophora alopecuroide of 1 ~ 5 weight portion, the piemarker of 1 ~ 5 weight portion, the castor bean of 0.5 ~ 6 weight portion and 1 ~ 6 weight portion ginkgo leaf;
Or 2 ~ 5 peganum harmala Ls of weight portion, the Tong fiber crops of 2 ~ 5 weight portions, the Sophora alopecuroide of 2 ~ 5 weight portions and the castor bean of 2 ~ 5 weight portions.
Wherein, above-mentioned raw materials is Chinese herbal medicine raw material, said herbal medicine plant material directly can be pulverized or becomes thoroughly decomposed by described active component, fermenting is prepared from, in order to improve the effect of root knot nematode control agent of the present invention, preferably, its active component to be got through ethanolic solution or water extraction by said herbal medicine plant material and is prepared from.
Further, in order to improve the activity of root knot nematode control agent, in addition to the active ingredient (s also preferably containing auxiliary material in described root knot nematode control agent, described auxiliary material can adopt the conventional auxiliary material in composition, such as adopts at least one in dispersant, wetting agent, bleeding agent, synergist; Preferably, described bleeding agent is at least one in fatty alcohol polyethenoxy ether class surfactant, organosilicon bleeding agent, Organic fluoride bleeding agent; Described dispersant is at least one in lignosulfonates, sldium lauryl sulfate, phosphate, alkylphenol-polyethenoxy base phosphate; Described wetting agent is at least one in alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester; Described synergist is organosilicon.Root knot nematode control agent of the present invention can be prepared into the common dosage forms such as aqua, pulvis or granule.
Further, in described pulvis and granule, also comprise carrier, the preferred maltose of described carrier, soluble starch or fertilizer element; Wherein, at least one in described fertilizer element preferably sulfuric acid potassium, ammonium sulfate, MAP, diammonium phosphate, magnesium sulfate, potassium dihydrogen phosphate or organic matter; Organic to become thoroughly decomposed at least one that thing, careless carbon, ight soil becomes thoroughly decomposed in thing etc. for agricultural crop straw.
Wherein, described root knot nematode control agent can adopt conventional method to prepare, such as can adopt and add auxiliary material and carrier in active component, stir, dry, pulverize to obtain pulvis, also can add in active component after filler drying makes powder prepare again, mix, dry, pulverize to obtain powder product.The preparation method of granule is: active component is mixed rear granulation, drying with filler, dispersant, wetting agent, synergist etc., sieve (1 ~ 4.75um), obtains granule.
Further, in order to improve the effect of root knot nematode control agent, root knot nematode control agent of the present invention preferably adopts following three kinds of methods preparation and obtains:
Method (one):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverizes;
B, immersion: the Chinese herbal medicine raw material after the pulverizing of a step is added in solvent and soaks, obtain immersion mixture, soak time preferably 12 ~ 36 hours; Wherein, described solvent is ethanol water, preferred 1:20 ~ 50 of mass ratio of described Chinese herbal medicine raw material and solvent, the mass ratio of water and ethanol preferably 0.25 ~ 4:1 in solvent;
C, extraction: ultrasonic lixiviate 30 ~ 60min under b step gained immersion mixture is preferable over 20 ~ 40 DEG C of conditions, obtains extract;
D, preparation: extract is made aqua, pulvis or granule.
Wherein, in order to improve the production efficiency of root knot nematode control agent, reduce production cost, soak time more preferably 18 ~ 30 hours, the mass ratio of Chinese herbal medicine raw material and solvent preferably more preferably 1:30 ~ 40, the mass ratio of water and ethanol preferred 0.5 ~ 1:1 further in solvent.
Wherein, for the ease of storage and transport, extract can be concentrated, obtain concentrate or medicinal extract, and then be prepared into various formulation; Cycles of concentration can according to needs of production from Row sum-equal matrix.
Method (two):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverizes;
B, preparation: pulvis made by the raw material after a step being pulverized.
Method (three):
A, fermentation maturity: by Chinese herbal medicine raw material hybrid concurrency ferment or become thoroughly decomposed;
B, preparation: the raw material after becoming thoroughly decomposed by fermentation is made pulvis or granule;
Wherein, active in order to improve root knot nematode control agent of the present invention, preferably, ferment through row again after Chinese herbal medicine raw material is pulverized or become thoroughly decomposed.
Further, the invention also discloses the application of root knot nematode control agent in control plant root-knot nematode.In order to improve the effect of root knot nematode control agent, the use age is preferably seedling stage of plant; Root knot nematode control agent of the present invention can be used by common method, as poured into a mould, spraying, spray, dusting, distribution, its amount of application with weather condition or crop condition change.General, adopt the methods such as cast, spraying, when using by root knot nematode control agent thin up of the present invention to suitable concentration, concentration can regulate according to needs of production, amount of application preferably 2 ~ 8L/ mu/time.Preferably aqua is adopted and be sprayed on plant blade face, being more preferably when plant premorbid or their early stage, by doubly spraying the soil around pouring crop root after aqua dilution, after being preferable over 10 ~ 15 days, carrying out secondary dispenser, the preferred continuous administration of one season crop 2 ~ 3 times, amount of application is 3 ~ 8L/ mu.For pulvis or granule except rear pouring can be watered, sprays by above-mentioned extension rate, rewater after also can spreading fertilizer over the fields or mix soil, the place making active ingredient can be distributed to crop root as much as possible to reach.Also can spread fertilizer over the fields the soil of crop, scatter, mix soil before and after crops be transplanted, make worm's ovum or larva suppressed or kill, amount of application is also by 2 ~ 8 kgs/acre times, use by 2 ~ 4 kgs/acre times at Seedling Stage, with the occurring degree of root-knot nematode and insect density determination consumption 4-8 kg/acre after 10 ~ 15 days.
Root knot nematode control agent of the present invention can be widely used in the plant that can be subject to preventing and treating root-knot nematode disease, namely root knot nematode control agent of the present invention may be used for prevention plant root-knot nematode evil, or for being subject to the plant of root-knot nematode evil, the common plant being subject to root-knot nematode evil has water spinach, cucumber, tomato, carrot, Chinese cabbage, lettuce, eggplant, custard squash etc.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
The preparation of embodiment 1 root knot nematode control agent of the present invention
A, pulverizing: raw material is mixed according to the herbal raw material component in table 1 and pulverizes;
B, immersion: the herbal raw material after pulverizing is added in ethanol water and soaks, obtains immersion mixture, wherein, herbal raw material and solvent proportion, ethanol water concentration are in table 2;
C, extraction: by the ultrasonic lixiviate of b step gained immersion mixture, obtaining final product, extraction conditions is in table 3;
D, preparation: extract is made aqua, pulvis or granule.
Table 1 is group raw material and proportioning thereof respectively
| Numbering | Component and quality thereof |
| gh-01 | Peganum harmala L: Sophora alopecuroide: piemarker: castor bean: ginkgo leaf=10g:10g:10g:5g:10g |
| gh-02 | Peganum harmala L: Sophora alopecuroide: piemarker: castor bean: ginkgo leaf=30g:30g:30g:35g:30g |
| gh-03 | Peganum harmala L: Sophora alopecuroide: piemarker: castor bean: ginkgo leaf=50g:50g:50g:60g:60g |
| gh-04 | Peganum harmala L: Tong fiber crops: Sophora alopecuroide: castor bean=20g:20g:50g:30g |
| gh-05 | Peganum harmala L: Tong fiber crops: Sophora alopecuroide: castor bean=20g:50g:30g:40g |
| gh-06 | Peganum harmala L: Tong fiber crops: Sophora alopecuroide: castor bean=50g:50g:50g:50g |
Continued 1
Table 2 is group raw material soaking condition respectively
Table 3 respectively organizes extraction conditions
| Numbering | Temperature (DEG C) | Extraction time (min) |
| gh-01 | 20 | 30 |
| gh-02 | 25 | 35 |
| gh-03 | 29 | 40 |
| gh-04 | 32 | 45 |
| gh-05 | 35 | 50 |
Continued 3
| gh-06 | 38 | 55 |
| gh-07 | 40 | 60 |
| gh-08 | 30 | 45 |
| gh-09 | 30 | 45 |
| gh-10 | 30 | 45 |
The preparation of embodiment 2 root knot nematode control agent of the present invention
1, gh-01 is concentrated into 10% of extract, obtains gh-01 concentrate, get the gh-01 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-01 aqua.
2, gh-02 is concentrated into 5% of extract, obtains gh-02 concentrate, get the gh-02 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-02 aqua.
3, gh-03 is concentrated into 15% of extract, obtains gh-03 concentrate, get the gh-03 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-03 aqua.
4, gh-04 is concentrated into 18% of extract, obtains gh-04 concentrate, get the gh-04 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-04 aqua.
5, gh-05 is concentrated into 20% of extract, obtains gh-05 concentrate, get the gh-05 concentrate of 10g, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, stirring, dry, pulverizing, obtain GH-05 pulvis.
6, gh-06 is concentrated into 15% of extract, obtains gh-06 concentrate, get the gh-06 concentrate of 10, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, stirring, dry, pulverizing, obtain GH-06 pulvis.
7, gh-07 is concentrated into 15% of extract, obtains gh-07 concentrate, get the gh-07 concentrate of 10, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, drying, excessively 1um sieve, obtain GH-07 granule.
8, gh-08 is concentrated into 15% of extract, obtains gh-08 concentrate, get the gh-08 concentrate of 10, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, drying, excessively 4.75um sieve, obtain GH-08 granule.
9, gh-09 is concentrated into 15% of extract, obtains gh-07 concentrate, get the gh-07 concentrate of 10, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, drying, excessively 1um sieve, obtain GH-09 granule.
10, gh-10 is concentrated into 15% of extract, obtains gh-08 concentrate, get the gh-08 concentrate of 10, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, drying, excessively 4.75um sieve, obtain GH-10 granule.
Embodiment 3 root knot nematode control agent of the present invention water spinach field efficacy is tested
1, experiment material
For studying thing: water spinach;
Reagent agent: GH-01;
Contrast medicament: 0.5% Avermectin particle Fushan City, Guangdong Province Ying Hui crop science Co., Ltd
2, experimental technique
2.1 reagent agent experimental scheme
Chemicals treatment, amount of application, time of application etc. are in table 4.
Table 4 reagent agent experimental scheme
Test site: Chongli town, Dongpo District, Meishan city
Weather condition: duration of test temperature 19-31 DEG C.Maintain an equal level, without special hazard weather in precipitation and former years therebetween.
Other factors: consumption is consistent as far as possible to water each region when being watered solution, and other fertilizer, water, disease, worm, crop smothering management are unanimously.
2.2 Quarter Design
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement, and permutation table sees the following form.
Table 5 community permutation table
2.3 investigation methods, content
2.3.1 strain morbidity survey
The method of investigation and sampling has multiple, according to actual conditions, and Yin Qiegen percentage method: do not extract plant, be easy to practical operation, therefore this test adopts this method.Dig the root of 1/2nd during pilot survey with scuppit, range estimation root nodule length sum accounts for the percentage of total root length.Concrete grade scale is: 0 grade, without visible root nodule or pieces of an egg; 1 grade, root nodule total length accounts for 1%-24% of total root length; 2 grades, root nodule total length accounts for 25%-49% of total root length; 3 grades, root nodule total length accounts for 50%-74% of total root length; 4 grades, root nodule total length accounts for 75%-100% of total root length.Adopt accidental sampling, every community random searching 5 point, often 2 strain water spinaches, prize root with instrument, observe root root-knot nematode incidence, statistics morbidity amount; Calculate root knot exponential sum control efficiency.
2.3.2 conventional record
On impact and the root knot morbidity classification situation of growth.
2.3.3 control time and number of times
Crop growth period carry out 2 times investigation, be respectively second time medication after 7 days and third time medication after 15 days.
2.3.4 drug effect computational methods
2.3.5 on the direct impact of crop
Observe medicament to crop with or without poisoning, the type of record poisoning and degree.In addition, other beneficial effects (as promoted maturation, stimulating growth etc.) to crop will also be recorded.
With the poisoning of following manner record:
If a) poisoning can measured or calculate, represent with absolute figure, as plant height.
B) in other cases, degree and the frequency of poisoning can be estimated by following two kinds of methods;
1) according to the poisoning situation of the every community of poisoning pulvis method record, represent with following stage division.
Poisoning stage division:
0 grade: without poisoning;
1 grade: slight poisoning, does not affect crop normal growth;
2 grades: moderate poisoning, can restore, can not crop failure be caused;
3 grades: severe poisoning, affects normal growth, loss is to a certain degree caused to crop yield and quality;
4 grades: serious poisoning, plant growth is obstructed, and seed output and quality loss is serious.
2) chemicals treatment district is compared with blank district, evaluate its poisoning percentage.
Meanwhile, the symptom of chemical damage (dwarfing, chlorisis, deformity etc.) of accurate description crop.
2.3.6 on the impact of other biological
Record medicament in test block on the impact of other biological.
2.3.7 control time, number of times
The plot that this test is selected be root-knot nematode occur ground, investigation carried out twice, be respectively after second time dispenser 10 days and third time medication after 15 days investigation incidence.
3 test results and analysis
3.1 result of the test
2 investigation have been carried out in this test, and 5 points are got in every community, adopt Qie Genfa investigation, and investigation initial data is in table 5, and test process the data obtained is through statistics, and its disease index and preventive effect, in table 6.
Table 6 test data table
Continued 6
Table 7 medicament is to the preventive effect of root-knot nematode
3.2 interpretation of result
As can be seen from the test results, GH-01 tri-concentration process water spinaches and soil, certain preventive effect is all had to control root-knot nematode, after second time medication, after 10 days and third time medication, investigation in 15 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-01 to control water spinach has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Effect experiment between embodiment 4 root knot nematode control agent of the present invention corn field
1, experiment material
For studying thing: corn;
Reagent agent: GH-02;
Contrast medicament: 0.5% Avermectin particle Fushan City, Guangdong Province Ying Hui crop science Co., Ltd
2, experimental technique
2.1 reagent agent experimental scheme
Chemicals treatment, amount of application, time of application etc. are in table 8.
Table 8 reagent agent experimental scheme
Test site: 5 groups, township, southwest, Pujiang County, Sichuan Province, this ground cutworm, mole cricket are caused harm seriously.
Weather condition: duration of test temperature 14-30 DEG C.Maintain an equal level, without special hazard weather in precipitation and former years therebetween.
Other factors: consumption is consistent as far as possible to water each region when being watered solution, and other fertilizer, water, disease, worm, crop smothering management are unanimously.
2.2 Quarter Design
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement, and arranging situation sees the following form.
Table 9 community permutation table
2.3 investigation methods, content
2.3.1 strain morbidity survey
This test select experimental field for cutworm, mole cricket are caused harm serious place, investigate the growing way of mainly maize seedling, cutworm, the survival volume of mole cricket and the Harm for corn.
2.3.2 conventional record
On the impact of corn growth and affect situation to maize seedling, and cutworm, mole cricket survival volume carry out investigating and record.
2.3.3 control time and number of times
Crop growth period carry out 2 times investigation, be respectively first time medication after 7 days and second time medication after 7 days.
(asking inventor to examine corn control time)
2.3.4 drug effect computational methods
2.3.5 on the direct impact of crop
Observe medicament to crop with or without poisoning, the type of record poisoning and degree.In addition, other beneficial effects (as promoted maturation, stimulating growth etc.) to crop will also be recorded.
With the poisoning of following manner record:
If a) poisoning can measured or calculate, represent with absolute figure, as plant height.
B) in other cases, degree and the frequency of poisoning can be estimated by following two kinds of methods;
1) according to the poisoning situation of the every community of poisoning pulvis method record, represent with following stage division.
Poisoning stage division:
0 grade: without poisoning;
1 grade: slight poisoning, does not affect crop normal growth;
2 grades: moderate poisoning, can restore, can not crop failure be caused;
3 grades: severe poisoning, affects normal growth, loss is to a certain degree caused to crop yield and quality;
4 grades: serious poisoning, plant growth is obstructed, and seed output and quality loss is serious.
2) chemicals treatment district is compared with blank district, evaluate its poisoning percentage.
Meanwhile, the symptom of chemical damage (dwarfing, chlorisis, deformity etc.) of accurate description crop.
2.3.6 on the impact of other biological
Record medicament in test block on the impact of other biological.
2.3.7 control time, number of times
The plot that this test is selected be root-knot nematode occur ground, investigation carried out twice, be respectively first time medication after 7 days and second time medication after 7 days.
3 test results and analysis
3.1 result of the test
2 investigation have been carried out in this test, and 5 points are got in every community, adopt Qie Genfa investigation, and test process the data obtained is through statistics, and its disease index and preventive effect, in table 10.
Table 10 medicament is to the preventive effect of root-knot nematode
3.2 interpretation of result
As can be seen from the test results, GH-02 tri-concentration process corns and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 7 days and second time medication, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, this test institute with medicament GH-02 root-knot nematode to control of maize has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Effect experiment between embodiment 5 root knot nematode control agent of the present invention Peanut Fields
Effect experiment between the Peanut Fields adopting the method for embodiment 4 to carry out root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in table 11.
Table 11 reagent agent experimental scheme
2 Quarter Designs
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement, and arranging situation sees the following form.
Table 12 community permutation table
3 experimental results
Adopt the investigation method of embodiment 4, poisoning stage division, data processing method, within 10 days after first time dispenser, carry out first time data survey, within 10 days, carry out second time data survey after second time dispenser, preventive effect the results are shown in Table 13.
Table 13 medicament is to the preventive effect of root-knot nematode
3.1 interpretation of result
As can be seen from the test results, GH-03 tri-concentration process corns and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 10 days and second time medication, investigation in 10 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-03 to control peanut has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Embodiment 6 root knot nematode control agent of the present invention pea field efficacy is tested
The method of embodiment 4 is adopted to carry out the pea field efficacy experiment of root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in table 14.
Table 14 reagent agent experimental scheme
2 Quarter Designs
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement, and arranging situation sees the following form.
Table 15 community permutation table
3 experimental results
Adopt the investigation method of embodiment 4, poisoning stage division and data processing method, within 7 days after first time dispenser, carry out first time data survey, within 7 days, carry out second time data survey after second time dispenser, preventive effect the results are shown in Table 16.
Table 16 medicament is to the preventive effect of root-knot nematode
3.1 interpretation of result
As can be seen from the test results, GH-04 tri-concentration process peas and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 7 days and second time medication, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-04 to control pea has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Embodiment 7 root knot nematode control agent of the present invention carrot field efficacy is tested
Effect experiment between the Peanut Fields adopting the method for embodiment 4 to carry out root knot nematode control agent.
1 experimental pharmacy and disposition thereof are in table 17.
Table 17 reagent agent experimental scheme
2 Quarter Designs
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement, and arranging situation sees the following form.
Table 18 community permutation table
3 experimental results
Adopt the investigation method of embodiment 4, poisoning stage division and data processing method, within 7 days after first time dispenser, carry out first time data survey, within 7 days, carry out second time data survey after second time dispenser, preventive effect the results are shown in Table 19.
Table 19 medicament is to the preventive effect of root-knot nematode
3.1 interpretation of result
As can be seen from the test results, GH-05 tri-concentration process carrots and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 7 days and second time medication, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-05 to control carrot has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Embodiment 8 root knot nematode control agent of the present invention Chinese cabbage field efficacy is tested
The method of embodiment 4 is adopted to carry out the Chinese cabbage field efficacy experiment of root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in table 20.
Table 20 reagent agent experimental scheme
Continued 20
2 Quarter Designs
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement.
Table 21 community permutation table
3 experimental results
Adopt the investigation method of embodiment 4, poisoning stage division and data processing method, within 7 days after first time dispenser, carry out first time data survey, within 7 days, carry out second time data survey after second time dispenser, preventive effect the results are shown in Table 22.
Table 22 medicament is to the preventive effect of root-knot nematode
3.1 interpretation of result
As can be seen from the test results, GH-06 tri-concentration process Chinese cabbages and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 7 days and second time medication, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-06 to control Chinese cabbage has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Embodiment 9 root knot nematode control agent of the present invention lettuce field efficacy is tested
The method of embodiment 4 is adopted to carry out the lettuce field efficacy experiment of root knot nematode control agent.
1 experimental pharmacy and disposition thereof are in table 23.
Table 23 reagent agent experimental scheme
2 Quarter Designs
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement, and arranging situation sees the following form.
Table 24 community permutation table
3 experimental results
Adopt the investigation method of embodiment 4, poisoning stage division and data processing method, within 10 days after first time dispenser, carry out first time data survey, within 10 days, carry out second time data survey after second time dispenser, preventive effect the results are shown in Table 25.
Table 25 medicament is to the preventive effect of root-knot nematode
3.1 interpretation of result
As can be seen from the test results, GH-07 tri-concentration process lettuces and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 10 days and second time medication, investigation in 10 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-07 to control peanut has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Effect experiment between embodiment 10 root knot nematode control agent of the present invention eggplant field
Effect experiment between the eggplant field adopting the method for embodiment 4 to carry out root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in table 26.
Table 26 reagent agent experimental scheme
Continued 26
2 Quarter Designs
This test medicament, three concentration, 1 contrast medicament, 1 blank, repeats for three times, Gong15Ge community, each community 30m
2.Minizone and surrounding all leave isolation strip, and community respectively processes randomized arrangement.
Table 27 community permutation table
3 experimental results
Adopt the investigation method of embodiment 4, poisoning stage division and data processing method, within 10 days after first time dispenser, carry out first time data survey, within 10 days, carry out second time data survey after second time dispenser, preventive effect the results are shown in Table 28.
Table 28 medicament is to the preventive effect of root-knot nematode
3.1 interpretation of result
As can be seen from the test results, GH-08 tri-concentration process eggplants and soil, certain preventive effect is all had to control root-knot nematode, after first time medication, after 10 days and second time medication, investigation in 10 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, wherein by the better effects if of B and C process, but the preventive effect difference of two consumptions is not remarkable, considers from the angle of economy, uses can prevent and treat root-knot nematode preferably by 4 kgs/acre times.
4 conclusion (of pressure testing)s
In sum, the root-knot nematode of this test institute with medicament GH-08 to control eggplant has certain control efficiency, and drug effect is higher than Avermectin, and consumption uses by 4 kgs/acre times can reach desirable control efficiency.
Claims (6)
1. root knot nematode control agent, is characterized in that, its active component is prepared from by the raw material of following weight portion:
The asiatic moonseed of the Sophora alopecuroide of the peganum harmala L of 1 ~ 8 weight portion, the ginkgo leaf of 0.5 ~ 8 weight portion, 1 ~ 10 weight portion, the root of subprostrate sophora of 0 ~ 5 weight portion, 0 ~ 5 weight portion, the kuh-seng of 0 ~ 10 weight portion, the castor bean of 0 ~ 8 weight portion, the piemarker of 1 ~ 10 weight portion, the crowndaisy chrysanthemum of 3 ~ 8 weight portions, the datura of 1 ~ 5 weight portion;
Or 1 ~ 5 peganum harmala L of weight portion, the Sophora alopecuroide of 1 ~ 6 weight portion, the piemarker of 1 ~ 8 weight portion, the ginkgo leaf of 1 ~ 7 weight portion, the kuh-seng of 1 ~ 5 weight portion, the castor bean of 1 ~ 8 weight portion;
Or 1 ~ 5 peganum harmala L of weight portion, the Sophora alopecuroide of 1 ~ 5 weight portion, the piemarker of 1 ~ 5 weight portion, the castor bean of 0.5 ~ 6 weight portion and 1 ~ 6 weight portion ginkgo leaf;
Or 2 ~ 5 peganum harmala Ls of weight portion, the piemarker of 2 ~ 5 weight portions, the Sophora alopecuroide of 2 ~ 5 weight portions and 2 ~ 5 weight portions castor bean;
The preparation method of described root knot nematode control agent comprises the steps:
A, pulverizing: raw material is mixed and pulverizes;
B, immersion: added in solvent by the raw material after the pulverizing of a step and soak to obtain immersion mixture, soak time is 12 ~ 36 hours; Wherein, described solvent is ethanol water, and the mass ratio of described raw material and solvent is 1:20 ~ 50, and in solvent, the mass ratio of water and ethanol is 0.25 ~ 4:1;
C, extraction: by b step gained immersion mixture ultrasonic lixiviate 30 ~ 60min under 20 ~ 40 DEG C of conditions, obtain extract;
D, preparation: extract is made aqua, pulvis or granule.
2. root knot nematode control agent according to claim 1, is characterized in that: also comprise carrier in described pulvis and granule, described carrier is maltose, soluble starch or fertilizer element; Wherein, described fertilizer element is at least one in potassium sulfate, ammonium sulfate, MAP, diammonium phosphate, magnesium sulfate, potassium dihydrogen phosphate or organic matter; Organic to become thoroughly decomposed at least one that thing, careless carbon, ight soil becomes thoroughly decomposed in thing for agricultural crop straw.
3. root knot nematode control agent according to claim 1 and 2, is characterized in that, in described b step, soak time is 18 ~ 30 hours; The mass ratio of described raw material and solvent is 1:30 ~ 40, and in solvent, the mass ratio of water and ethanol is 0.5 ~ 1:1.
4. the application of the root knot nematode control agent described in any one of claims 1 to 3 in control plant root-knot nematode.
5. the application of root knot nematode control agent according to claim 4 in control plant root-knot nematode, is characterized in that: described root knot nematode control agent is used for being subject to the plant of root-knot nematode insect pest; Will aqua dilute after pour into a mould, spray on soil, or pulvis and granule to be dissolved in the water, to pour into a mould after dilution, spray on soil, or by pulvis or granule dusting, intersperse among in soil, rewater.
6. the application of root knot nematode control agent according to claim 5 in control plant root-knot nematode, is characterized in that: the plant that described meeting is subject to root-knot nematode insect pest is water spinach, cucumber, tomato, carrot, Chinese cabbage, lettuce, eggplant, custard squash.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310601194.6A CN103583606B (en) | 2013-11-21 | 2013-11-21 | Root knot nematode control agent and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310601194.6A CN103583606B (en) | 2013-11-21 | 2013-11-21 | Root knot nematode control agent and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103583606A CN103583606A (en) | 2014-02-19 |
| CN103583606B true CN103583606B (en) | 2016-02-17 |
Family
ID=50074095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310601194.6A Active CN103583606B (en) | 2013-11-21 | 2013-11-21 | Root knot nematode control agent and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103583606B (en) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430557A (en) * | 2014-11-15 | 2015-03-25 | 辽宁农业职业技术学院 | Chinese herbal medicine composition for preventing and treating plant root-knot nematode as well as preparation method and application thereof |
| CN105211129A (en) * | 2015-09-17 | 2016-01-06 | 张超 | A kind of composition pesticide of deinsectization ovum |
| CN105330461A (en) * | 2015-11-23 | 2016-02-17 | 杨丁元 | Biological medicine fertilizer for controlling plant root knot nematodes as well as preparation method and application of biological medicine fertilizer |
| CN105284937B (en) * | 2015-11-30 | 2018-03-09 | 南京中医药大学 | A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application |
| CN105646011A (en) * | 2016-01-11 | 2016-06-08 | 济南康众医药科技开发有限公司 | New application of trogopterus dung |
| CN105993493A (en) * | 2016-05-29 | 2016-10-12 | 寿光市新世纪种苗有限公司 | Biological trapping method for controlling colored bell pepper root-knot nematode |
| CN105875293A (en) * | 2016-06-07 | 2016-08-24 | 青川县宏宇猕猴桃种植专业合作社 | Kiwi fruit canker prevention method and prevention agent |
| CN106614814A (en) * | 2016-08-02 | 2017-05-10 | 安树义 | Production process of veratrine nematicide |
| CN106577796A (en) * | 2016-11-22 | 2017-04-26 | 山东省农业科学院蔬菜花卉研究所 | Method of prevention and treatment of tomato root knot nematode disease by castor bean meal |
| CN106889116A (en) * | 2017-02-27 | 2017-06-27 | 青岛海之星生物科技有限公司 | A kind of peanut root-knot nematode attractant and preparation method thereof |
| CN107512986A (en) * | 2017-10-03 | 2017-12-26 | 长沙仲善新能源科技有限公司 | Synergy environment-friendlyfertilizer fertilizer of improvement plantation forest soil and preparation method thereof |
| CN109452307B (en) * | 2018-11-27 | 2021-10-08 | 成都新朝阳作物科学股份有限公司 | Water-soluble biological organic fertilizer for preventing and treating root-knot nematode and preparation method thereof |
| CN109650997A (en) * | 2019-01-30 | 2019-04-19 | 沈阳禾康永丰生物环保产业有限公司 | A kind of plant root-knot nematode administering method |
| CN111567571B (en) * | 2020-05-15 | 2021-09-03 | 湖北大学 | Botanical compound insecticide for meloidogyne incognita and preparation method thereof |
| CN114158508B (en) * | 2021-11-30 | 2023-07-25 | 海南归耘田农业科技有限公司 | Method for effectively preventing crayfish from being infected by parasites |
| CN113994960A (en) * | 2021-12-01 | 2022-02-01 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009093079A2 (en) * | 2008-01-23 | 2009-07-30 | Arab Science And Technology Foundation | Environmentally friendly plant protection agents |
| CN102775238A (en) * | 2012-08-22 | 2012-11-14 | 乌鲁木齐木易联华生物科技有限公司 | Organic plant pesticide fertilizer with pesticidal and bactericidal effects and production technique thereof |
-
2013
- 2013-11-21 CN CN201310601194.6A patent/CN103583606B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009093079A2 (en) * | 2008-01-23 | 2009-07-30 | Arab Science And Technology Foundation | Environmentally friendly plant protection agents |
| CN102775238A (en) * | 2012-08-22 | 2012-11-14 | 乌鲁木齐木易联华生物科技有限公司 | Organic plant pesticide fertilizer with pesticidal and bactericidal effects and production technique thereof |
Non-Patent Citations (6)
| Title |
|---|
| 不同植物提取物对马铃薯茎线虫的活性筛选;闫磊 等;《山东农业大学学报(自然科学版)》;20081231;第39卷(第2期);第223-228页 * |
| 杀线虫植物的筛选及对根结线虫防治作用的研究;邱雪柏;《中国优秀硕士学位论文全文数据库农业科技辑》;20111231(第S1期);摘要、正文 * |
| 植物源农药防治根结线虫研究进展;刘计权 等;《农药》;20110630;第50卷(第6期);第395-398页 * |
| 苘麻提取物对南方根结线虫生物活性测定及活性成份的分离;谢宁;《山东农业大学硕士学位论文》;20130406;摘要、正文 * |
| 苦豆子甲醇提取物对南方根结线虫的生物活性;杜小凤 等;《江西农业学报》;20101231;第22卷(第11期);第88-90页 * |
| 茶籽粗提物杀线虫活性初步研究;徐晓莉;《中国优秀硕士学位论文全文数据库农业科技辑》;20081015(第10期);摘要、正文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103583606A (en) | 2014-02-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103583606B (en) | Root knot nematode control agent and its preparation method and application | |
| CN105580843A (en) | Brassinolide containing spore pesticide fertilizer and preparation method thereof | |
| CN109041624A (en) | A kind of Degraded Alpine grassland ecology after-culture restorative procedure | |
| CN103058779B (en) | Pyridine.benzene thiamethoxam acyl fertilizer and production method thereof | |
| CN105211072A (en) | Herbicidal combinations | |
| KR20110033691A (en) | Environment-friendly organic agricultural products for soil insect pest control | |
| CN103918651A (en) | Pesticide composition for preventing and treating root knot nematode disease and method | |
| CN102204564B (en) | Pesticide composition containing fosthiazate and carbosulfan | |
| CN106212154A (en) | Karst Rocky Desertification mountain region macadimia nut cultural method | |
| CN104817406A (en) | Plant protection composition containing chitosan oligosaccharide and dinotefuran | |
| CN109369256A (en) | Composite microbiological fertilizer with insecticidal activity | |
| CN109232074A (en) | Water soluble fertilizer with activity of weeding | |
| CN102972430A (en) | Insecticidal composition containing thiamethoxam and acetamiprid | |
| CN104996228A (en) | Comprehensive control method for tomato prodenia litura | |
| CN106954634A (en) | A kind of composition pesticide and its application containing pyraclonil and anilofos | |
| CN106508956A (en) | Nematicidal composition containing trifluoro-pyrimidine amide and thiazole phosphine | |
| CN106118675A (en) | Biogas residue sour soil modifying agent and using method thereof | |
| CN101558757A (en) | Insecticidal and acaricidal composition containing spirodiclofen and nitenpyram | |
| CN107771623A (en) | A kind of high-yield planting method of bamboo shoots | |
| CN109548793A (en) | One group of novel peanut special medicated fertilizer | |
| CN104904735B (en) | A kind of herbicidal composition and its application containing pinoxaden | |
| CN102599181A (en) | Oil suspension agent composition containing nicosulfuron and 2,4-d isooctyl ester | |
| CN113973855A (en) | High-efficiency low-toxicity low-residue cucumber root knot nematode disease control agent | |
| CN106472196A (en) | A kind of Oryza sativa L. deinsectization cultural method | |
| CN104430581A (en) | Molluscicide composition, bensulfuron methyl and butachlor-containing herbicidal and molluscicidal labor-saving fertilizer for rice and production method of labor-saving fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |