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I process 6 different samples that I mapped against the same reference genome (the mitochondria) using Dorado. Using Samtool coverage, I checked that all my sample covered 100% of my genome with with a minimum depth of 1. I then used modkit to generate a bedMethyl table and it turns out that my 6 samples have different number of rows.
Using modkit, I used the option --filter-threshold.
Does bedMethyl table output only site that have at least 1 called higher than the threshold ?
So that there is no lines like
Nmod = 0
Ncanonical = 0
Nother mod = 0
Nvalid_cov = 0
Nfail = 15
Thank you !
The text was updated successfully, but these errors were encountered:
Does bedMethyl table output only site that have at least 1 called higher than the threshold ?
So that there is no lines like
Nmod = 0
Ncanonical = 0
Nother mod = 0
Nvalid_cov = 0
Nfail = 15
That's correct, as mentioned in the documentation, rows with Nvalid_cov = 0 are not reported. If you have a use case where this is a problem I could add a flag in the next release that outputs positions where all of the modification calls fall below the pass threshold.
Hello,
I process 6 different samples that I mapped against the same reference genome (the mitochondria) using Dorado. Using Samtool coverage, I checked that all my sample covered 100% of my genome with with a minimum depth of 1. I then used modkit to generate a bedMethyl table and it turns out that my 6 samples have different number of rows.
Using modkit, I used the option --filter-threshold.
Does bedMethyl table output only site that have at least 1 called higher than the threshold ?
So that there is no lines like
Nmod = 0
Ncanonical = 0
Nother mod = 0
Nvalid_cov = 0
Nfail = 15
Thank you !
The text was updated successfully, but these errors were encountered: