• Windows OS (see Camstim package)
• python 2.7
• psychopy 1.82.01
• camstim 0.2.4

1. Navigate to repository and install conda environment.
   conda env create -f environment.yml
2. Activate the environment.
   conda activate allen_stimulus
3. Install the AIBS camstim package in the environment.
   pip install camstim/.
4. Download required video clips from movie_clips.zip Extract into the data directory.

The software requires two sets of input files. There should be a set of text files present under data/stimulus_orderings that indicate the display order of video clips for different phases of the experiment. In addition, there should be a set of video clips (stored as raw .npy files). These clips must be downloaded and extracted into the data folder from movie_clips.zip

1. For the random stimulus order (days #0 and #5):

   • there are 4 difference choices of a 2 sec duration stimulus
     • movie clip A
     • movie clip B
     • movie clip C
     • a constant grey screen, X
   • these will be displayed in a randomized order.
   • this order will be exactly the same on day #0 and day #5.
   • there will be 525 repeats of each of the 4 stimuli.

2. For the sequence stimulus order (day #1 – #4):

   • the 3 movie clips are shown in the same repeated order, ABC, for 50 minutes.
   • this will result in 500 repeats of this movie clip sequence.
   • in the last 20 minutes, the stimuli will be shown in a random order with the grey screen intermixed, as on days #0 and #5
   • a different random sequence will be chosen and kept the same across days #1 – #4.
   • this will result in 150 repeats of each of these 4 stimuli.