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NGS Core Facility

The NGS Core Facility provides highly parallel DNA sequencing services based on the Illumina MiSeq, NextSeq and NovaSeq technologies as well as on the Oxford Nanopore technologies.

 

Applications

• DNA Sequencing

  • Whole Genome Sequencing
  • Targeted Resequencing (e.g. Exome Sequencing)
  • De-Novo Sequencing

• RNA-Sequencing

  • mRNA stranded
  • UltraLowRNA
  • RiboZero

• Bisulfite Sequencing

  • Analysis of DNA methylation patterns via Whole Genome

 

Our services

• Assistance in experimental design
• Sample preparation starting from customer prepared DNA/RNA
• Sequencing service for customer libraries and multiplexes
• Sequence analysis on Illumina:
  • MiSeq (50, 300 and 500 cycles kit on V2 flowcell; 150 and 600 cycles kit on V3 flowcell)
  • NextSeq 550 (75, 150 and 300 cycles kit on MidOutput flowcell; 150 and 300 cycles kit on HighOutput flowcell)
  • NovaSeq 6000 (100, 200, 300 and 500 cycles kit on SP flowcell; 100, 200 and 300 cycles kit on S1 and S2 flowcell; 35, 200 and 300 cycles kit on S4 flowcell)
  • NovaSeq X Plus (100, 200 and 300 cycles kit on 1.B and 10B flowcell; 300 cycles kit on 25B flowcell)
• Provision of the full sequence data
• Basic bioinformatics support for the analysis of resulting sequence data
• Sequencing Open Lab
  • Illumina technologies:
    • NextSeq 550
    • NextSeq 2000
  • Oxford Nanopore technologies:
    • GridION
    • PromethION

 

For detailed information about the our service, different platforms as well as pooling recommendation please check our FAQs in ILSe or contact Dr. Stephan Wolf or Dr. Angela Schulz.