Papers by Luis Monteagudo
Se han analizado muestras individuales correspondientes a 105 perdices rojas (Alectoris rufa ) cr... more Se han analizado muestras individuales correspondientes a 105 perdices rojas (Alectoris rufa ) criadas en cautividad y pertenecientes a 10 explotaciones españolas distintas, dedicadas a la cría de perdices para repoblación.
Italian Journal of Animal Science, 2013
ABSTRACT Number and population size of local chicken breeds in Italy is considered to be critical... more ABSTRACT Number and population size of local chicken breeds in Italy is considered to be critical. Molecular data can be used to provide reliable insight into the diversity of chicken breeds. The first aim of this study was to investigate the maternal genetic origin of five Italian local chicken breeds (Ancona, Livorno, Modenese, Romagnola and Valdarnese bianca) based on mitochondrial DNA (mtDNA) information. Secondly, the extent of the genetic diversity, population structure and the genetic relation-ships among these chicken populations, by using 27 microsatellite markers, were assessed. To achieve these targets, a 506 bp fragment of the D-loop region was sequenced in 50 chickens of the five breeds. Eighteen variable sites were observed which defined 12 haplotypes. They were assigned to three clades and two maternal lineages. Results indicated that 90% of the haplotypes are related to clade E, which has been described to originate from the Indian subcontinent. For the microsatellite analysis, 137 individual blood samples from the five Italian breeds were included. A total of 147 alleles were detected at 27 microsatellite loci. The five Italian breeds showed a slightly higher degree of inbreeding (FIS=0.08) than the commercial populations that served as ref-erence. Structure analysis showed a separa-tion of the Italian breeds from the reference populations. A further sub-clustering allowed discriminating among the five different Italian breeds. This research provides insight into population structure, relatedness and variabil-ity of the five studied breeds.
The Veterinary record, Jan 21, 2015
ABSTRACT
Journal of Heredity, 2007
10 In order to detect introgression of other Alectoris genus species into wild populations of Spa... more 10 In order to detect introgression of other Alectoris genus species into wild populations of Spanish Alectoris rufa, we studied a sample of 93 red-legged partridges (supposed to be A. rufa) captured in the island of Majorca. A set of 31 chukar partridges (Alectoris chukar) from Cyprus and 33 red-legged partridges (A. rufa) from one Spanish farm were also studied to provide suitable populations for comparison. Factorial correspondence analysis on microsatellite genotypes supported a clear distinction of birds from Cyprus, whereas partridges from Majorca and the Spanish farm overlapped in a wide area. The existence 15 of A. chukar mitochondrial DNA in 16 individuals from Majorca indicated introgression into their maternal lineage even if their phenotypes were not different from A. rufa. Bayesian inference based on microsatellite analysis indicated a noticeable degree of genetic proximity to A. chukar only for one of these hybrids. 65 individuals, bred in captivity in one Spanish farm (located in Catalonia), selected from a set of 28 collaborating continental
Genomics, 1993
The 19 chromosomal pairs of the swine karyotype are resolved into 18 peaks denoted A to Q and Y b... more The 19 chromosomal pairs of the swine karyotype are resolved into 18 peaks denoted A to Q and Y by dual-beam flow cytometry. The chromosomal content of six peaks has previously been determined by analyzing male/female differences, karyotypes of animals carrying translocations, and PCR studies of genes with known assignments. For the remaining chromosomes, putative assignments to flow peaks were deduced from comparison of DNA contents, determined by flow cytometry, and chromosomal size. We present here the complete characterization of the pig bivariate flow karyotype using the PARM-PCR technique combined with fluorescence in situ hybridization. Chromosome-specific probes were generated by PCR amplification of 300 sorted chromosomes with primers under nonspecific conditions and used to paint chromosomes by FISH. The chromosomal content of each peak was identified: peaks A (chromosome 1), B (13), C (6), D (2), E (14), F (3), G (7), H (9,4,X), H1 (9), I (15), J (8), K (5), L (10), M (12), N (16), O (11), P (17), Q (18), Y (Y). We were able to characterize perfectly the pig bivariate flow karyotype. Such techniques could be applied to any other species.
Journal of Heredity
... 21 Suiter KA, Wendel JF, and Case JS. ... Figure 1). We identified NOR-bearing regions in the... more ... 21 Suiter KA, Wendel JF, and Case JS. ... Figure 1). We identified NOR-bearing regions in the Spanish common rabbit as the telomeres of the short arms of pairs 13, 16, 20 and the telomere of the long arms of number 21, according to the re-sults published by Martin De Leon et al ...
Información técnica económica agraria
Se han analizado muestras individuales correspondientes a 105 perdices rojas (Alectoris rufa ) cr... more Se han analizado muestras individuales correspondientes a 105 perdices rojas (Alectoris rufa ) criadas en cautividad y pertenecientes a 10 explotaciones españolas distintas, dedicadas a la cría de perdices para repoblación.
Información técnica económica agraria
The Journal of Immunology, 2001
Preformed Fas ligand (FasL) and APO2 ligand (APO2L)/TNF-related apoptosis-inducing ligand (TRAIL)... more Preformed Fas ligand (FasL) and APO2 ligand (APO2L)/TNF-related apoptosis-inducing ligand (TRAIL) are stored in the cytoplasm of the human Jurkat T cell line and of normal human T cell blasts. The rapid release of these molecules in their bioactive form is involved in activation-induced cell death. In this study, we show by confocal microscopy that FasL and APO2L/TRAIL are mainly localized in lysosomal-like compartments in these cells. We show also by immunoelectron microscopy that FasL and APO2L/TRAIL are stored inside cytoplasmic compartments ϳ500 nm in diameter, with characteristics of multivesicular bodies. Most of these compartments share FasL and APO2L/TRAIL, although exclusive APO2L/TRAIL labeling can be also observed in separate compartments. Upon PHA activation, the mobilization of these compartments toward the plasma membrane is evident, resulting in the secretion of the internal microvesicles loaded with FasL and APO2L/TRAIL. In the case of activation with anti-CD59 mAb, the secretion of microvesicles labeled preferentially with APO2L/TRAIL predominates. These data provide the basis of a new and efficient mechanism for the rapid induction of autocrine or paracrine cell death during immune regulation and could modify the interpretation of the role of FasL and APO2L/TRAIL as effector mechanisms in physiological and pathological situations. Abbreviations used in this paper: AICD, activation-induced cell death; FasL, Fas ligand; APO2L, APO2 ligand; TRAIL, TNF-related apoptosis inducing ligand; MVB, multivesicular body; PLT, progressive lowering temperature.
Cytogenetics and cell genetics
Mammalian Genome
Species: Cattle Locus name: Transition Protein 1 Locus symbol: TNP1 Map position: Syntenyc group ... more Species: Cattle Locus name: Transition Protein 1 Locus symbol: TNP1 Map position: Syntenyc group number U17 Method of mapping: Somatic cell hybrids and PCR Molecular reagents: PCR primers: (+) 5'-GGCATGAG-GAGGGGCAAGAAC-3' and (-) 5'-TCACAAGTGGGA-GCGCAAATTG-3' loci. Previously identified homologs: Ovine TNP1, U11 synteny group [1] assigned to Chromosome (Chr) 2 [2]
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Papers by Luis Monteagudo