Shamsuddin et al., 1993 - Google Patents

In vitro development up to hatching of bovine in vitro-matured and fertilized oocytes with or without support from somatic cells

Shamsuddin et al., 1993

Document ID
3624810961424475806
Author
Shamsuddin M
Larsson B
Gustafsson H
Rodriguez-Martinez H
Publication year
Publication venue
Theriogenology

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To verify the importance of somatic cells upon in vitro embryo development, in vitro-matured (IVM) and-fertilized (IVF) bovine oocytes were cultured in TCM 199 supplemented with estrous cow serum (10% v/v) and 0.25 mM sodium pyruvate (ECSTCM) under the following …
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N2501/20—Cytokines; Chemokines
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