Gomis-Fons et al., 2019 - Google Patents

Integration of a complete downstream process for the automated lab-scale production of a recombinant protein

Gomis-Fons et al., 2019

Document ID
17167910041388038102
Author
Gomis-Fons J
Löfgren A
Andersson N
Nilsson B
Berghard L
Wood S
Publication year
Publication venue
Journal of Biotechnology

External Links

Snippet

In this work, an automated downstream process for the purification and formulation of a recombinant protein was integrated at lab scale in a single chromatography unit. The purification chain consists of three bind-and-elute chromatography columns, a flow-through …
Continue reading at www.sciencedirect.com (other versions)

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1814—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns recycling of the fraction to be distributed
    • B01D15/1821—Simulated moving beds
    • B01D15/1828—Simulated moving beds characterized by process features
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase

Similar Documents

Publication Publication Date Title
Gomis-Fons et al. Integration of a complete downstream process for the automated lab-scale production of a recombinant protein
Steinebach et al. Design and operation of a continuous integrated monoclonal antibody production process
Arnold et al. Implementation of fully integrated continuous antibody processing: effects on productivity and COGm
EP2480305B1 (en) Separation system and method
JP7196263B2 (en) Process control system and method for use in filters and filtration processes
Karst et al. Process performance and product quality in an integrated continuous antibody production process
Gomis-Fons et al. Optimization study on periodic counter-current chromatography integrated in a monoclonal antibody downstream process
Narayanan et al. Integration and digitalization in the manufacturing of therapeutic proteins
Ötes et al. Scale-up of continuous multicolumn chromatography for the protein a capture step: from bench to clinical manufacturing
Schwarz et al. Integrated continuous biomanufacturing on pilot scale for acid‐sensitive monoclonal antibodies
Ötes et al. Feasibility of using continuous chromatography in downstream processing: Comparison of costs and product quality for a hybrid process vs. a conventional batch process
Hughson et al. Development of a 3‐step straight‐through purification strategy combining membrane adsorbers and resins
Milne Scale-up of protein purification: downstream processing issues
Shamashkin et al. A tandem laboratory scale protein purification process using Protein A affinity and anion exchange chromatography operated in a weak partitioning mode
Ebeler et al. One-step integrated clarification and purification of a monoclonal antibody using Protein A Mag Sepharose beads and a cGMP-compliant high-gradient magnetic separator
Dutta et al. Performance optimization of continuous countercurrent tangential chromatography for antibody capture
Eggersgluess et al. Integration of aqueous two‐phase extraction into downstream processing
Dutta et al. Continuous countercurrent tangential chromatography for mixed mode post-capture operations in monoclonal antibody purification
Persson et al. Design of two-column batch-to-batch recirculation to enhance performance in ion-exchange chromatography
Vogg et al. Continuous and integrated expression and purification of recombinant antibodies
Kozorog et al. Model-based process optimization for mAb chromatography
Qu et al. The transition from resin chromatography to membrane adsorbers for protein separations at industrial scale
Thakur et al. Continuous manufacturing of monoclonal antibodies: Automated downstream control strategy for dynamic handling of titer variations
US10717023B1 (en) Method for continuous purification
Jagschies Continuous capture of mAbs—Points to consider and case studies