Tulchin et al., 1976 - Google Patents

A microgel system for disc electrophoresis

Tulchin et al., 1976

Document ID
163354975154846482
Author
Tulchin N
Ornstein L
Davis B
Publication year
Publication venue
Analytical biochemistry

External Links

Snippet

Disc electrophoresis has provided a suitable method for separating macromolecules (Davis, 1964; Ornstein, 1964). Numerous authors (2, 5–10, 12) have presented scaled-down versions which were not directly comparable to the standard separations. In the present …
Continue reading at www.sciencedirect.com (other versions)

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44752—Controlling the zeta potential, e.g. by wall coatings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44708—Cooling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay
    • G01N33/558—Immunoassay; Biospecific binding assay using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/42—Measuring disposition or liberation of materials from an electrolyte; Coulometry, i.e. measuring coulomb-equivalent of material in an electrolyte
    • G01N27/423—Coulometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/92—Construction of the plate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Similar Documents

Publication Publication Date Title
Moss et al. Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes: A new method for the separation of polypeptide subunits
US3384564A (en) Electrophoretic process for simultaneously spearating and concentrating particles
Garrels [28] Quantitative two-dimensional gel electrophoresis of proteins
Clarke Simplified “disc”(polyacrylamide gel) electrophoresis
Shimura et al. Affinity capillary electrophoresis: a sensitive tool for the study of molecular interactions and its use in microscale analyses
Neville Jr et al. [9] Molecular weight determination of membrane protein and glycoprotein subunits by discontinuous gel electrophoresis in dodecyl sulfate
Bravo Two-dimensional gel electrophoresis: a guide for the beginner
JP3122846B2 (en) Polymers for separation of biomolecules by capillary electrophoresis
Choules et al. An acrylamide gel soluble in scintillation fluid: its application to electrophoresis at neutral and low pH
Robertson et al. Rapid isoelectric focusing in a vertical polyacrylamide minigel system
Maizel Jr Polyacrylamide gel electrophoresis of viral proteins
US5085756A (en) Column separation system for electrophoresis with sample pretreatment
Shi et al. One-dimensional polyacrylamide gel electrophoresis
US20060137982A1 (en) Polymers for separation of biomolecules by capillary electrophoresis
Tulchin et al. A microgel system for disc electrophoresis
Lorentz A simple polyacrylamide gradient gel preparation for estimating molecular weights
Nakagawa et al. SEPARATION AND DETERMINATION OF CEFPIRANIDE IN HUMAN PLASMA BY ELECTROKINETIC CHROMATOGRAPHY WITH A MICELLAR SOLUTION AND AN OPEN TUBULAR-FUSED SILICA CAPILLARY
US3620947A (en) Electrophoretic system
Pratt et al. Polyacrylamide gels of increasing concentration gradient for the electrophoresis of lipoproteins
Jeppesen [39] Separation and isolation of DNA fragments using linear polyacrylamide gradient gel electrophoresis
Kingsbury et al. On the determination of component molecular weights in complex protein mixtures by means of disc electrophoresis
Korant et al. Zonal electrophoresis and isoelectric focusing of proteins and virus particles in density gradients of small volume
Rabilloud Two‐dimensional electrophoresis of basic proteins with equilibrium isoelectric focusing in carrier ampholyte‐pH gradients
Roman et al. Micellar electrokinetic chromatography of fluorescently labeled proteins on poly (dimethylsiloxane)‐based microchips
Ferris et al. Hemoglobin electrophoresis in acrylamide gel