Bennett et al., 2014 - Google Patents

Library construction for ancient genomics: single strand or double strand?

Bennett et al., 2014

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Document ID
16240326224036142642
Author
Bennett E
Massilani D
Lizzo G
Daligault J
Geigl E
Grange T
Publication year
Publication venue
Biotechniques

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Snippet

A novel method of library construction that takes advantage of a single-stranded DNA ligase has been recently described and used to generate high-resolution genomes from ancient DNA samples. While this method is effective and appears to recover a greater fraction of …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for mutation or polymorphism detection
    • C12Q1/683—Hybridisation assays for mutation or polymorphism detection involving restriction enzymes, e.g. RFLP
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    • C12Q1/6844—Nucleic acid amplification reactions
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